Origin IGS: tttttaattatatatggtaattacttgacagcctataatgtttctcgaactcacaaaagctttttttaacattataggacatcacagtaatcttactaatgattgctataaagaaagggaaaaataaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttatttttccctttctttatagcaatcattagtaagattactgtgatgtcctataatgttaaaaaaagcttttgtgagttcgagaaacattataggctgtcaagtaattaccatatataattaaaaa Mask Tandem Repeat Region ================================================ tttttaattatatatggtaattacttgacagcctataatgtttctcgaactcacaaaagctttttttaacattataggacatcacagtaatcttactaatgattgctataaagaaagggaaaaataaa Find is-nt database================================================ Query_seq: FN0052:FN0053|FN0052:FN0053:Arsenate reductase:Branched-chain amino acid transport system carrier protein:->->:689352..689479 128 tttttaattatatatggtaattacttgacagcctataatgtttctcgaactcacaaaagctttttttaacattataggacatcacagtaatcttactaatgattgctataaagaaagggaaaaataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0052:FN0053|FN0052:FN0053:Arsenate reductase:Branched-chain amino acid transport system carrier protein:->->:689352..689479 128 tttttaattatatatggtaattacttgacagcctataatgtttctcgaactcacaaaagctttttttaacattataggacatcacagtaatcttactaatgattgctataaagaaagggaaaaataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0052:FN0053|FN0052:FN0053:Arsenate reductase:Branched-chain amino acid transport system carrier protein:->->:689352..689479 128 tttttaattatatatggtaattacttgacagcctataatgtttctcgaactcacaaaagctttttttaacattataggacatcacagtaatcttactaatgattgctataaagaaagggaaaaataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttttaattatatatggtaattacttgacagcctataatgtttctcgaactcacaaaagctttttttaacattataggacatcacagtaatcttactaatgattgctataaagaaagggaaaaataaa Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 35 End: 124 .................................. M M F L E L T K A F F N I I G H H S N L T N D C Y K E R E K * .... Protein_Len: 33 Strand: - Start: 11 End: 109 .......... I H Y N S S L R Y H K E F E C F S K K V N Y S M V T I K S I I A M ................... tttttaattatatatggtaattacttgacagcctataatgtttctcgaactcacaaaagctttttttaacattataggacatcacagtaatcttactaatgattgctataaagaaagggaaaaataaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttttaattatatatggtaattacttgacagcctataatgtttctcgaactcacaaaagctttttttaacattataggacatcacagtaatcttactaatgattgctataaagaaagggaaaaataaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0052:FN0053|FN0052:FN0053:Arsenate reductase:Branched-chain amino acid transport system carrier protein:->->:689352..689479 128 tttttaattatatatggtaattacttgacagcctataatgtttctcgaactcacaaaagctttttttaacattataggacatcacagtaatcttactaatgattgctataaagaaagggaaaaataaa Intra-Species Hit: Count: 1 Min: 1 Max: 128 Len: 128 Subject: NC_003454_FN0052_FN0053|Arsenate reductase:Branched-chain amino acid transport system carrier protein|POSITIVE:POSITIVE|[689352,689479]|128 HSP 1 e-value: 2.0E-54 bit: 212.0 Len: 128 Query Start:1 Query End:128 Subject Strand: POSITIVE Subject Start: 1 Subject End: 128 tttttaattatatatggtaattacttgacagcctataatgtttctcgaactcacaaaagcnnnnnnnaacattataggacatcacagtaatcttactaatgattgctataaagaaagggaaaaataaa tttttaattatatatggtaattacttgacagcctataatgtttctcgaactcacaaaagctttttttaacattataggacatcacagtaatcttactaatgattgctataaagaaagggaaaaataaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUAAUUAUAUAUGGUAAUUACUUGACAGCCUAUAAUGUUUCUCGAACUCACAAAAGCUUUUUUUAACAUUAUAGGACAUCACAGUAAUCUUACUAAUGAUUGCUAUAAAGAAAGGGAAAAAUAAA (((((..((...(((((((((((..(((...(((((((((((....(((((......)).)))....)))))))))))...))).((((....))))..)))))))))))...))..)))))...... (-24.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUAUUUUUCCCUUUCUUUAUAGCAAUCAUUAGUAAGAUUACUGUGAUGUCCUAUAAUGUUAAAAAAAGCUUUUGUGAGUUCGAGAAACAUUAUAGGCUGUCAAGUAAUUACCAUAUAUAAUUAAAAA ....................................(((((((.(((((.(((((((((((......(((((....))))).....))))))))))).)))))))))))).................. (-24.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 506 80 33 207 161 NC_003454:FN2091|5end_hypothetical_protein_602481..602711_NEGATIVE 260 70 24 163 118 NC_003454:FN0695|5end_ABC_transporter_ATP-binding_protein_1352886..1353116_POSITIVE 249 67 25 208 149 NC_003454:FN1935|5end_Adenine-specific_methyltransferase_437061..437291_NEGATIVE 222 68 21 195 142 NC_003454:FN1551|5end_hypothetical_protein_66187..66417_NEGATIVE 220 60 22 228 192 NC_003454:FN0481|5end_hypothetical_protein_1127777..1128007_POSITIVE 201 120 86 56 11 NC_003454:FN1177|5end_hypothetical_protein_1838805..1839035_NEGATIVE 199 56 24 134 97 NC_003454:FN0418|5end_pyrimidine_regulatory_protein_PyrR_1059118..1059348_POSITIVE 196 69 26 85 46 NC_003454:FN1266|5end_UTP--glucose-1-phosphate_uridylyltransferase_1923063..1923293_NEGATIVE 191 65 31 150 112 NC_003454:FN1451|5end_cell_division_protein_FtsZ_2132710..2132940_NEGATIVE 189 84 43 126 86 NC_003454:FN1368|5end_COMF_operon_protein_3_2018771..2019001_NEGATIVE 186 68 25 172 127 NC_003454:FN0854|5end_Glucose-1-phosphate_adenylyltransferase_1513487..1513717_NEGATIVE 186 64 21 142 91 NC_003454:FN1631|5end_SSU_ribosomal_protein_S14P_135247..135477_NEGATIVE 185 75 31 63 20 NC_003454:FN0773|5end_hypothetical_protein_1429274..1429504_POSITIVE 184 89 59 110 78 NC_003454:FN1154|5end_Ribonuclease_BN_1808232..1808462_POSITIVE 183 44 12 191 161 NC_003454:FN1707|5end_Aldose_1-epimerase_202181..202411_POSITIVE 181 75 36 169 129 NC_003454:FN1153|5end_hypothetical_protein_1807827..1808057_POSITIVE 177 107 62 139 94 NC_003454:FN0922|5end_Homoserine_kinase_1577526..1577756_NEGATIVE 175 79 37 118 73 NC_003454:FN1862|5end_L-beta-lysine_5,6-aminomutase_beta_subunit_353122..353352_NEGATIVE 174 111 75 93 50 NC_003454:FN1379|5end_Citrate_lyase_beta_chain_2026355..2026585_POSITIVE 174 68 26 112 64 NC_003454:FN1917|5end_tRNA_delta(2)-isopentenylpyrophosphate_transferase_413756..413986_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 506 80 33 74 28 NC_003454:FN2092|3end_Integral_membrane_protein_602614..602764_NEGATIVE 417 80 32 57 10 NC_003454:FN0829|3end_hypothetical_protein_1489580..1489730_NEGATIVE 302 80 31 59 11 NC_003454:FN1076|3end_hypothetical_protein_1723677..1723827_POSITIVE 267 79 32 68 22 NC_003454:FN0828|3end_ABC_transporter_permease_protein_1489505..1489655_POSITIVE 260 70 24 61 16 NC_003454:FN0694|3end_S-layer_protein_1352864..1353014_POSITIVE 249 67 25 127 68 NC_003454:FN1936|3end_hypothetical_protein_437142..437292_NEGATIVE 206 89 44 48 1 NC_003454:FN1260|3end_Sensory_Transduction_Protein_Kinase_1915863..1916013_NEGATIVE 201 120 86 68 23 NC_003454:FN1178|3end_Hypothetical_cytosolic_protein_1838793..1838943_NEGATIVE 196 69 26 94 55 NC_003454:FN1267|3end_hypothetical_protein_1923054..1923204_NEGATIVE 191 65 31 127 89 NC_003454:FN1452|3end_Cell_division_protein_ftsA_2132733..2132883_NEGATIVE 190 98 55 76 27 NC_003454:FN0052|3end_Arsenate_reductase_689291..689441_POSITIVE 189 84 43 118 78 NC_003454:FN1369|3end_Zinc_finger_protein_2018779..2018929_NEGATIVE 186 116 71 101 61 NC_003454:FN0935|3end_hypothetical_protein_1587558..1587708_NEGATIVE 186 68 25 151 106 NC_003454:FN0855|3end_glucose-1-phosphate_adenylyltransferase_1513508..1513658_NEGATIVE 186 64 21 121 70 NC_003454:FN1632|3end_LSU_ribosomal_protein_L5P_135268..135418_NEGATIVE 184 89 59 34 2 NC_003454:FN1153|3end_hypothetical_protein_1808236..1808386_POSITIVE 183 92 57 68 35 NC_003454:FN0273|3end_Butyrate-acetoacetate_CoA-transferase_subunit_B_900162..900312_POSITIVE 181 75 36 149 109 NC_003454:FN1152|3end_aspartate_aminotransferase_1807887..1808037_POSITIVE 180 92 57 76 43 NC_003454:FN1077|3end_hypothetical_protein_1723754..1723904_NEGATIVE 178 52 21 126 91 NC_003454:FN1602|3end_Hypothetical_cytosolic_protein_111022..111172_NEGATIVE