Origin IGS: gatattttaaaagataaggtatttccagatacaaaaaatgagaatttaagtgaagacaaaagaacatactcagtattctggcatatagattcaaaagataaaataggaccttatatttcaataaatttttatacagaacattccaaaggagatataaaaccaagatatagagaagatgaatataataaattatttaatatcaaaagaaaaatgtgataaattagttgaatgtattagaataatagatagagaattaagaaaataataaatacaaaaaagtttcaggaagtttttatttacttaaatataaaaatatggtattttatatttaaggaggataaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttatcctccttaaatataaaataccatatttttatatttaagtaaataaaaacttcctgaaacttttttgtatttattattttcttaattctctatctattattctaatacattcaactaatttatcacatttttcttttgatattaaataatttattatattcatcttctctatatcttggttttatatctcctttggaatgttctgtataaaaatttattgaaatataaggtcctattttatcttttgaatctatatgccagaatactgagtatgttcttttgtcttcacttaaattctcattttttgtatctggaaataccttatcttttaaaatatc Mask Tandem Repeat Region ================================================ gatattttaaaagataaggtatttccagatacaaaaaatgagaatttaagtgaagacaaaagaacatactcagtattctggcatatagattcaaaagataaaataggaccttatatttcaataaatttttatacagaacattccaaaggagatataaaaccaagatatagagaagatgaatataataaattatttaatatcaaaagaaaaatgtgataaattagttgaatgtattagaataatagatagagaattaagaaaataataaatacaaaaaagtttcaggaagtttttatttacttaaatataaaaatatggtattttatatttaaggaggataaaa Find is-nt database================================================ Query_seq: FN0016:FN0017|FN0016:FN0017:hypothetical protein:hypothetical protein:->->:654427..654769 343 gatattttaaaagataaggtatttccagatacaaaaaatgagaatttaagtgaagacaaaagaacatactcagtattctggcatatagattcaaaagataaaataggaccttatatttcaataaatttttatacagaacattccaaaggagatataaaaccaagatatagagaagatgaatataataaattatttaatatcaaaagaaaaatgtgataaattagttgaatgtattagaataatagatagagaattaagaaaataataaatacaaaaaagtttcaggaagtttttatttacttaaatataaaaatatggtattttatatttaaggaggataaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0016:FN0017|FN0016:FN0017:hypothetical protein:hypothetical protein:->->:654427..654769 343 gatattttaaaagataaggtatttccagatacaaaaaatgagaatttaagtgaagacaaaagaacatactcagtattctggcatatagattcaaaagataaaataggaccttatatttcaataaatttttatacagaacattccaaaggagatataaaaccaagatatagagaagatgaatataataaattatttaatatcaaaagaaaaatgtgataaattagttgaatgtattagaataatagatagagaattaagaaaataataaatacaaaaaagtttcaggaagtttttatttacttaaatataaaaatatggtattttatatttaaggaggataaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0016:FN0017|FN0016:FN0017:hypothetical protein:hypothetical protein:->->:654427..654769 343 gatattttaaaagataaggtatttccagatacaaaaaatgagaatttaagtgaagacaaaagaacatactcagtattctggcatatagattcaaaagataaaataggaccttatatttcaataaatttttatacagaacattccaaaggagatataaaaccaagatatagagaagatgaatataataaattatttaatatcaaaagaaaaatgtgataaattagttgaatgtattagaataatagatagagaattaagaaaataataaatacaaaaaagtttcaggaagtttttatttacttaaatataaaaatatggtattttatatttaaggaggataaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gatattttaaaagataaggtatttccagatacaaaaaatgagaatttaagtgaagacaaaagaacatactcagtattctggcatatagattcaaaagataaaataggaccttatatttcaataaatttttatacagaacattccaaaggagatataaaaccaagatatagagaagatgaatataataaattatttaatatcaaaagaaaaatgtgataaattagttgaatgtattagaataatagatagagaattaagaaaataataaatacaaaaaagtttcaggaagtttttatttacttaaatataaaaatatggtattttatatttaaggaggataaaa Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 4 End: 213 ... M L K D K V F P D T K N E N L S E D K R T Y S V F W H I D S K D K I G P Y I S I N F Y T E H S K G D I K P R Y R E D E Y .................................................................................................................................. Protein_Len: 32 Strand: + Start: 167 End: 262 ...................................................................................................................................................................... M E K M N I I N Y L I S K E K C D K L V E C I R I I D R E L R K ................................................................................. Protein_Len: 30 Strand: + Start: 253 End: 342 ............................................................................................................................................................................................................................................................ M K K I I N T K K F Q E V F I Y L N I K I W Y F I F K E D K . Protein_Len: 31 Strand: - Start: 193 End: 285 ................................................................................................................................................................................................ K I D F S F H S L N T S H I L I I S L S N L F Y Y I C F L K M .......................................................... Protein_Len: 33 Strand: - Start: 132 End: 230 ................................................................................................................................... V S C E L P S I F G L Y L S S S Y L L N N L I L L F I H Y I L Q M ................................................................................................................. Protein_Len: 34 Strand: - Start: 3 End: 104 .. I K F S L T N G S V F F S F K L S S L L V Y E T N Q C I S E F S L M ............................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 2 End: 193 . Y P I E L Y L F H S N L H L C F F M S L I R A Y L N L L Y F L V K Y K L L N K Y L V N W L L Y L V L I Y L L H I Y Y I M ...................................................................................................................................................... gatattttaaaagataaggtatttccagatacaaaaaatgagaatttaagtgaagacaaaagaacatactcagtattctggcatatagattcaaaagataaaataggaccttatatttcaataaatttttatacagaacattccaaaggagatataaaaccaagatatagagaagatgaatataataaattatttaatatcaaaagaaaaatgtgataaattagttgaatgtattagaataatagatagagaattaagaaaataataaatacaaaaaagtttcaggaagtttttatttacttaaatataaaaatatggtattttatatttaaggaggataaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gatattttaaaagataaggtatttccagatacaaaaaatgagaatttaagtgaagacaaaagaacatactcagtattctggcatatagattcaaaagataaaataggaccttatatttcaataaatttttatacagaacattccaaaggagatataaaaccaagatatagagaagatgaatataataaattatttaatatcaaaagaaaaatgtgataaattagttgaatgtattagaataatagatagagaattaagaaaataataaatacaaaaaagtttcaggaagtttttatttacttaaatataaaaatatggtattttatatttaaggaggataaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0016:FN0017|FN0016:FN0017:hypothetical protein:hypothetical protein:->->:654427..654769 343 gatattttaaaagataaggtatttccagatacaaaaaatgagaatttaagtgaagacaaaagaacatactcagtattctggcatatagattcaaaagataaaataggaccttatatttcaataaatttttatacagaacattccaaaggagatataaaaccaagatatagagaagatgaatataataaattatttaatatcaaaagaaaaatgtgataaattagttgaatgtattagaataatagatagagaattaagaaaataataaatacaaaaaagtttcaggaagtttttatttacttaaatataaaaatatggtattttatatttaaggaggataaaa Intra-Species Hit: Count: 1 Min: 1 Max: 343 Len: 343 Subject: NC_003454_FN0016_FN0017|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[654427,654769]|343 HSP 1 e-value: 0.0 bit: 680.0 Len: 343 Query Start:1 Query End:343 Subject Strand: POSITIVE Subject Start: 1 Subject End: 343 gatattttaaaagataaggtatttccagatacaaaaaatgagaatttaagtgaagacaaaagaacatactcagtattctggcatatagattcaaaagataaaataggaccttatatttcaataaatttttatacagaacattccaaaggagatataaaaccaagatatagagaagatgaatataataaattatttaatatcaaaagaaaaatgtgataaattagttgaatgtattagaataatagatagagaattaagaaaataataaatacaaaaaagtttcaggaagtttttatttacttaaatataaaaatatggtattttatatttaaggaggataaaa gatattttaaaagataaggtatttccagatacaaaaaatgagaatttaagtgaagacaaaagaacatactcagtattctggcatatagattcaaaagataaaataggaccttatatttcaataaatttttatacagaacattccaaaggagatataaaaccaagatatagagaagatgaatataataaattatttaatatcaaaagaaaaatgtgataaattagttgaatgtattagaataatagatagagaattaagaaaataataaatacaaaaaagtttcaggaagtttttatttacttaaatataaaaatatggtattttatatttaaggaggataaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUAUUUUAAAAGAUAAGGUAUUUCCAGAUACAAAAAAUGAGAAUUUAAGUGAAGACAAAAGAACAUACUCAGUAUUCUGGCAUAUAGAUUCAAAAGAUAAAAUAGGACCUUAUAUUUCAAUAAAUUUUUAUACAGAACAUUCCAAAGGAGAUAUAAAACCAAGAUAUAGAGAAGAUGAAUAUAAUAAAUUAUUUAAUAUCAAAAGAAAAAUGUGAUAAAUUAGUUGAAUGUAUUAGAAUAAUAGAUAGAGAAUUAAGAAAAUAAUAAAUACAAAAAAGUUUCAGGAAGUUUUUAUUUACUUAAAUAUAAAAAUAUGGUAUUUUAUAUUUAAGGAGGAUAAAA (((((((((.....)))))))))(((.........((((((((((((...(((.(((...........(((..(((((((....(((.((((((..(((......((...(((((((((....(((.(((.....))).)))......)))))))))..))..(((((........((((((........)))))))))))..................)))..)))))).)))))))))).......))).((((......))))............))))))..)))))))))))).(((((((((((((........)))))))))))))..)))..... (-47.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUAUCCUCCUUAAAUAUAAAAUACCAUAUUUUUAUAUUUAAGUAAAUAAAAACUUCCUGAAACUUUUUUGUAUUUAUUAUUUUCUUAAUUCUCUAUCUAUUAUUCUAAUACAUUCAACUAAUUUAUCACAUUUUUCUUUUGAUAUUAAAUAAUUUAUUAUAUUCAUCUUCUCUAUAUCUUGGUUUUAUAUCUCCUUUGGAAUGUUCUGUAUAAAAAUUUAUUGAAAUAUAAGGUCCUAUUUUAUCUUUUGAAUCUAUAUGCCAGAAUACUGAGUAUGUUCUUUUGUCUUCACUUAAAUUCUCAUUUUUUGUAUCUGGAAAUACCUUAUCUUUUAAAAUAUC ((((((....(((((((((((((........)))))))))))))...)))))).............(((((((((....((((....((((.........))))....)))).................(((((((......(((((.((((.......((((...........)))).....)))).)))))......)))))))...)))))))))....((((((.(((((((.........((....)).........(((((.(((.(((.(((....((((........))))....))).))).))))))))....))))))).))))))...... (-42.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 188 288 243 226 189 NC_003454:FN0260|5end_Transcriptional_repressor_smtB_886638..886868_NEGATIVE 187 120 76 138 98 NC_003454:FN0304|5end_Oxidoreductase,_NIFE-related_938767..938997_POSITIVE 182 178 135 48 9 NC_003454:FN1542|5end_1,4-dihydroxy-2-naphthoate_octaprenyltransferase_56192..56422_POSITIVE 181 32 1 190 151 NC_003454:FN0911|5end_Alpha-ribazole-5'-phosphate_phosphatase_1569318..1569548_NEGATIVE 180 33 12 74 55 NC_003454:FN1155|5end_Cell_division_protein_ftsI_1809442..1809672_POSITIVE 178 117 72 163 116 NC_003454:FN0239|5end_hypothetical_protein_863655..863885_POSITIVE 177 289 245 59 14 NC_003454:FN1344|5end_hypothetical_protein_1997520..1997750_POSITIVE 177 119 71 117 64 NC_003454:FN0132|5end_Hemolysin_761715..761945_POSITIVE 176 60 12 64 19 NC_003454:FN1262|5end_Integral_membrane_protein_1918043..1918273_POSITIVE 175 195 163 163 131 NC_003454:FN0272|5end_Acetoacetate:butyrate/acetate_coenzyme_A_transferase_898704..898934_POSITIVE 175 93 57 101 62 NC_003454:FN1617|5end_Prolipoprotein_diacylglyceryltransferase_126666..126896_NEGATIVE 174 154 116 221 179 NC_003454:FN0346|5end_hypothetical_protein_979267..979497_NEGATIVE 173 177 147 173 135 NC_003454:FN0534|5end_hypothetical_protein_1188858..1189088_POSITIVE 173 162 125 223 192 NC_003454:FN0340|5end_Hypothetical_membrane-spanning_Protein_973948..974178_NEGATIVE 173 29 7 50 25 NC_003454:FN2104|5end_hypothetical_protein_614237..614467_POSITIVE 173 146 109 60 25 NC_003454:FN1703|5end_ADP-L-glycero-D-manno-heptose-6-epimerase_198992..199222_NEGATIVE 171 186 142 46 3 NC_003454:FN1031|5end_Hypothetical_membrane-spanning_protein_1685800..1686030_NEGATIVE 168 176 131 222 182 NC_003454:FN1046|5end_hypothetical_protein_1696371..1696601_POSITIVE 168 99 66 117 84 NC_003454:FN1932|5end_Dephospho-CoA_kinase_432999..433229_NEGATIVE 163 337 291 211 159 NC_003454:FN0729|5end_Phosphoglycerate_mutase_1386354..1386584_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 191 298 257 63 23 NC_003454:FN0377|3end_Iron(III)-transport_system_permease_protein_sfuB_1009196..1009346_POSITIVE 190 181 142 49 8 NC_003454:FN0927|3end_hypothetical_protein_1581693..1581843_NEGATIVE 188 288 243 85 48 NC_003454:FN0261|3end_Pyruvate_formate-lyase_activating_enzyme_886779..886929_NEGATIVE 182 178 135 51 12 NC_003454:FN1541|3end_Heptaprenyl_diphosphate_synthase_component_II_56275..56425_POSITIVE 180 33 12 54 35 NC_003454:FN1154|3end_Ribonuclease_BN_1809502..1809652_POSITIVE 177 300 255 98 52 NC_003454:FN1855|3end_Methylaspartate_mutase_345486..345636_POSITIVE 175 195 163 100 68 NC_003454:FN0271|3end_Enoyl-CoA_hydratase_898721..898871_POSITIVE 175 93 57 101 62 NC_003454:FN1618|3end_hypothetical_protein_126666..126816_NEGATIVE 173 112 76 135 100 NC_003454:FN0675|3end_60_kDa_chaperonin_GROEL_1330471..1330621_NEGATIVE 173 162 125 118 87 NC_003454:FN0341|3end_transport_protein_974053..974203_NEGATIVE 172 110 70 137 91 NC_003454:FN2064|3end_hypothetical_protein_575213..575363_NEGATIVE 172 262 227 39 4 NC_003454:FN1797|3end_Spermidine/putrescine_transport_ATP-binding_protein_potA_280031..280181_POSITIVE 170 258 211 55 5 NC_003454:FN1276|3end_hypothetical_protein_1935280..1935430_POSITIVE 168 99 66 116 83 NC_003454:FN1933|3end_hypothetical_protein_433000..433150_NEGATIVE 167 246 202 43 4 NC_003454:FN0390|3end_hypothetical_protein_1028067..1028217_NEGATIVE 161 154 113 114 81 NC_003454:FN1714|3end_hypothetical_protein_211036..211186_POSITIVE 161 179 140 47 13 NC_003454:FN1560|3end_hypothetical_protein_82479..82629_NEGATIVE 160 260 211 119 61 NC_003454:FN0797|3end_hypothetical_protein_1455668..1455818_NEGATIVE 160 129 82 145 99 NC_003454:FN2023|3end_Hypothetical_cytosolic_protein_526172..526322_NEGATIVE 160 150 105 75 31 NC_003454:FN1668|3end_Cholinephosphate_cytidylyltransferase_164691..164841_NEGATIVE