Origin IGS: tcaaatattatttaacttctataaagactggttttattccagtcttttttgctgaaaaaatttttaaaatttactattaatttttttatatattcttattgtcaagtttattgtcttgtcaataaaaatttttagtgctataatctcctcaggaaattttgctataaaatgttttaggaggagta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tactcctcctaaaacattttatagcaaaatttcctgaggagattatagcactaaaaatttttattgacaagacaataaacttgacaataagaatatataaaaaaattaatagtaaattttaaaaattttttcagcaaaaaagactggaataaaaccagtctttatagaagttaaataatatttga Mask Tandem Repeat Region ================================================ tcaaatattatttaacttctataaagactggttttattccagtcttttttgctgaaaaaatttttaaaatttactattaatttttttatatattcttattgtcaagtttattgtcttgtcaataaaaatttttagtgctataatctcctcaggaaattttgctataaaatgttttaggaggagta Find is-nt database================================================ Query_seq: FN0007:FN0008|FN0007:FN0008:glucose-inhibited division protein A:quinolinate synthetase:->->:647965..648149 185 tcaaatattatttaacttctataaagactggttttattccagtcttttttgctgaaaaaatttttaaaatttactattaatttttttatatattcttattgtcaagtttattgtcttgtcaataaaaatttttagtgctataatctcctcaggaaattttgctataaaatgttttaggaggagta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0007:FN0008|FN0007:FN0008:glucose-inhibited division protein A:quinolinate synthetase:->->:647965..648149 185 tcaaatattatttaacttctataaagactggttttattccagtcttttttgctgaaaaaatttttaaaatttactattaatttttttatatattcttattgtcaagtttattgtcttgtcaataaaaatttttagtgctataatctcctcaggaaattttgctataaaatgttttaggaggagta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0007:FN0008|FN0007:FN0008:glucose-inhibited division protein A:quinolinate synthetase:->->:647965..648149 185 tcaaatattatttaacttctataaagactggttttattccagtcttttttgctgaaaaaatttttaaaatttactattaatttttttatatattcttattgtcaagtttattgtcttgtcaataaaaatttttagtgctataatctcctcaggaaattttgctataaaatgttttaggaggagta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tcaaatattatttaacttctataaagactggttttattccagtcttttttgctgaaaaaatttttaaaatttactattaatttttttatatattcttattgtcaagtttattgtcttgtcaataaaaatttttagtgctataatctcctcaggaaattttgctataaaatgttttaggaggagta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 28 End: 132 ........................... M V L F Q S F L L K K F L K F T I N F F I Y S Y C Q V Y C L V N K N F ..................................................... Protein_Len: 35 Strand: + Start: 80 End: 184 ............................................................................... M F L Y I L I V K F I V L S I K I F S A I I S S G N F A I K C F R R S . Protein_Len: 33 Strand: - Start: 67 End: 165 .................................................................. F K S N I K K Y I R I T L N I T K D I F I K L A I I E E P F K A M .................... tcaaatattatttaacttctataaagactggttttattccagtcttttttgctgaaaaaatttttaaaatttactattaatttttttatatattcttattgtcaagtttattgtcttgtcaataaaaatttttagtgctataatctcctcaggaaattttgctataaaatgttttaggaggagta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 91 Start: 115 End: 144 ..................................................................................................................CTTGTCAATAAAAATTTTTAGTGCTATAAT......................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tcaaatattatttaacttctataaagactggttttattccagtcttttttgctgaaaaaatttttaaaatttactattaatttttttatatattcttattgtcaagtttattgtcttgtcaataaaaatttttagtgctataatctcctcaggaaattttgctataaaatgttttaggaggagta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0007:FN0008|FN0007:FN0008:glucose-inhibited division protein A:quinolinate synthetase:->->:647965..648149 185 tcaaatattatttaacttctataaagactggttttattccagtcttttttgctgaaaaaatttttaaaatttactattaatttttttatatattcttattgtcaagtttattgtcttgtcaataaaaatttttagtgctataatctcctcaggaaattttgctataaaatgttttaggaggagta Intra-Species Hit: Count: 1 Min: 1 Max: 185 Len: 185 Subject: NC_003454_FN0007_FN0008|glucose-inhibited division protein A:quinolinate synthetase|POSITIVE:POSITIVE|[647965,648149]|185 HSP 1 e-value: 3.0E-48 bit: 192.0 Len: 97 Query Start:89 Query End:185 Subject Strand: POSITIVE Subject Start: 89 Subject End: 185 ........................................................................................tatattcttattgtcaagtttattgtcttgtcaataaaaatttttagtgctataatctcctcaggaaattttgctataaaatgttttaggaggagta ........................................................................................tatattcttattgtcaagtttattgtcttgtcaataaaaatttttagtgctataatctcctcaggaaattttgctataaaatgttttaggaggagta HSP 2 e-value: 7.0E-9 bit: 61.9 Len: 31 Query Start:1 Query End:31 Subject Strand: POSITIVE Subject Start: 1 Subject End: 31 tcaaatattatttaacttctataaagactgg.......................................................................................................................................................... tcaaatattatttaacttctataaagactgg.......................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCAAAUAUUAUUUAACUUCUAUAAAGACUGGUUUUAUUCCAGUCUUUUUUGCUGAAAAAAUUUUUAAAAUUUACUAUUAAUUUUUUUAUAUAUUCUUAUUGUCAAGUUUAUUGUCUUGUCAAUAAAAAUUUUUAGUGCUAUAAUCUCCUCAGGAAAUUUUGCUAUAAAAUGUUUUAGGAGGAGUA ......(((((...(((.....(((((((((.......))))))))).....((((((((((..((........))..)))))))))).......((((((.((((........)))).))))))........)))...)))))((((((..(((((.((.......)).)))))..)))))).. (-37.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACUCCUCCUAAAACAUUUUAUAGCAAAAUUUCCUGAGGAGAUUAUAGCACUAAAAAUUUUUAUUGACAAGACAAUAAACUUGACAAUAAGAAUAUAUAAAAAAAUUAAUAGUAAAUUUUAAAAAUUUUUUCAGCAAAAAAGACUGGAAUAAAACCAGUCUUUAUAGAAGUUAAAUAAUAUUUGA ............(((.(((((((......(((.(((((((((((............((((((((((.((((........)))).)))))))))).......((((((.......))))))...))))))))))).))).((((((((.......))))))))))))))))))............. (-34.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 262 150 101 60 11 NC_003454:FN0366|5end_Phosphoacetylglucosamine_mutase_996366..996596_NEGATIVE 228 60 16 213 169 NC_003454:FN1923|5end_Adenine-specific_methyltransferase_421097..421327_NEGATIVE 226 163 129 118 81 NC_003454:FN1823|5end_Transcriptional_regulator,_TetR_family_311720..311950_POSITIVE 225 175 131 60 21 NC_003454:FN1166|5end_Galactoside_transport_ATP-binding_protein_mglA_1824013..1824243_POSITIVE 224 45 8 71 30 NC_003454:FN1703|5end_ADP-L-glycero-D-manno-heptose-6-epimerase_198992..199222_NEGATIVE 221 180 138 87 36 NC_003454:FN1582|5end_hypothetical_protein_94957..95187_NEGATIVE 218 52 16 103 67 NC_003454:FN1454|5end_D-alanine--D-alanine_ligase_2135659..2135889_NEGATIVE 214 150 107 102 48 NC_003454:FN0288|5end_Hypoxanthine-guanine_phosphoribosyltransferase_915765..915995_NEGATIVE 214 53 17 160 111 NC_003454:FN2023|5end_Hypothetical_cytosolic_protein_526642..526872_NEGATIVE 213 118 71 169 112 NC_003454:FN2021|5end_ABC1_family_protein_525079..525309_NEGATIVE 210 179 131 161 122 NC_003454:FN1100|5end_Plasmid_addiction_system_poison_protein_1744177..1744407_POSITIVE 206 54 11 102 54 NC_003454:FN0304|5end_Oxidoreductase,_NIFE-related_938767..938997_POSITIVE 205 175 134 221 180 NC_003454:FN1895|5end_hypothetical_protein_382871..383101_NEGATIVE 205 53 15 59 24 NC_003454:FN1616|5end_transcription_antitermination_protein_NusB_126437..126667_NEGATIVE 203 55 18 125 87 NC_003454:FN1236|5end_Choline_transport_protein_1893480..1893710_NEGATIVE 202 47 11 55 17 NC_003454:FN1284|5end_30S_ribosomal_protein_S4_1943243..1943473_NEGATIVE 202 149 109 57 7 NC_003454:FN2082|5end_Formate--tetrahydrofolate_ligase_594891..595121_NEGATIVE 201 156 115 222 181 NC_003454:FN1386|5end_SWF/SNF_family_helicase_2042866..2043096_NEGATIVE 198 56 15 113 71 NC_003454:FN0357|5end_ATP_synthase_epsilon_chain,_sodium_ion_specific_988276..988506_NEGATIVE 195 150 132 218 201 NC_003454:FN2079|5end_ABC_transporter_ATP-binding_protein_591118..591348_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 226 163 129 55 18 NC_003454:FN1822|3end_Flavodoxin_311737..311887_POSITIVE 219 120 83 117 77 NC_003454:FN1811|3end_Manganese_transport_system_ATP-binding_protein_mntA_292161..292311_NEGATIVE 218 52 16 88 52 NC_003454:FN1455|3end_UDP-N-acetylenolpyruvoylglucosamine_reductase_2135674..2135824_NEGATIVE 208 153 127 127 100 NC_003454:FN0254|3end_Fusobacterium_outer_membrane_protein_family_881390..881540_POSITIVE 205 53 15 54 19 NC_003454:FN1617|3end_Prolipoprotein_diacylglyceryltransferase_126442..126592_NEGATIVE 202 55 17 123 86 NC_003454:FN1282|3end_LSU_ribosomal_protein_L17P_1941269..1941419_NEGATIVE 198 56 15 103 61 NC_003454:FN0358|3end_ATP_synthase_subunit_B_988286..988436_NEGATIVE 195 165 126 102 63 NC_003454:FN1828|3end_LSU_ribosomal_protein_L9P_317251..317401_NEGATIVE 193 124 88 50 16 NC_003454:FN1369|3end_Zinc_finger_protein_2018779..2018929_NEGATIVE 192 51 18 144 114 NC_003454:FN1237|3end_Choline_kinase_1893453..1893603_NEGATIVE 190 150 111 85 35 NC_003454:FN0175|3end_Cell_division_inhibitor_MinC_801235..801385_POSITIVE 189 153 127 127 100 NC_003454:FN2058|3end_Fusobacterium_outer_membrane_protein_family_565711..565861_NEGATIVE 189 153 127 127 100 NC_003454:FN2047|3end_Fusobacterium_outer_membrane_protein_family_551491..551641_NEGATIVE 189 153 127 127 100 NC_003454:FN1554|3end_Fusobacterium_outer_membrane_protein_family_68719..68869_NEGATIVE 188 141 107 129 101 NC_003454:FN1224|3end_2-dehydro-3-deoxyphosphooctonate_aldolase_1882540..1882690_NEGATIVE 188 89 41 102 52 NC_003454:FN0396|3end_Dipeptide-binding_protein_1035818..1035968_POSITIVE 185 168 134 120 70 NC_003454:FN0081|3end_Ethanolamine_utilization_protein_eutL_720768..720918_POSITIVE 185 79 32 57 18 NC_003454:FN1615|3end_Integral_membrane_protein_125756..125906_POSITIVE 182 43 6 129 89 NC_003454:FN0916|3end_hypothetical_protein_1572065..1572215_NEGATIVE 182 122 81 122 70 NC_003454:FN0611|3end_Threonyl-tRNA_synthetase_1270301..1270451_POSITIVE