Origin IGS: attcaatggataatggattaggggaggtacagcccctaatccgacgaaaggcgacgacgcagaggctatactttatcctgaggcggcaccggaaaaaccttcaattgacataaggcttattcttctatatacttattttcataggaggactt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aagtcctcctatgaaaataagtatatagaagaataagccttatgtcaattgaaggtttttccggtgccgcctcaggataaagtatagcctctgcgtcgtcgcctttcgtcggattaggggctgtacctcccctaatccattatccattgaat Mask Tandem Repeat Region ================================================ attcaatggataatggattaggggaggtacagcccctaatccgacgaaaggcgacgacgcagaggctatactttatcctgaggcggcaccggaaaaaccttcaattgacataaggcttattcttctatatacttattttcataggaggactt Find is-nt database================================================ Query_seq: TDE2737:TDE2738|TDE2737:TDE2738:hypothetical protein:oligoendopeptidase F, putative:->->:2792751..2792902 152 attcaatggataatggattaggggaggtacagcccctaatccgacgaaaggcgacgacgcagaggctatactttatcctgaggcggcaccggaaaaaccttcaattgacataaggcttattcttctatatacttattttcataggaggactt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE2737:TDE2738|TDE2737:TDE2738:hypothetical protein:oligoendopeptidase F, putative:->->:2792751..2792902 152 attcaatggataatggattaggggaggtacagcccctaatccgacgaaaggcgacgacgcagaggctatactttatcctgaggcggcaccggaaaaaccttcaattgacataaggcttattcttctatatacttattttcataggaggactt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE2737:TDE2738|TDE2737:TDE2738:hypothetical protein:oligoendopeptidase F, putative:->->:2792751..2792902 152 attcaatggataatggattaggggaggtacagcccctaatccgacgaaaggcgacgacgcagaggctatactttatcctgaggcggcaccggaaaaaccttcaattgacataaggcttattcttctatatacttattttcataggaggactt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attcaatggataatggattaggggaggtacagcccctaatccgacgaaaggcgacgacgcagaggctatactttatcctgaggcggcaccggaaaaaccttcaattgacataaggcttattcttctatatacttattttcataggaggactt Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 6 End: 110 ..... M D N G L G E V Q P L I R R K A T T Q R L Y F I L R R H R K N L Q L T .......................................... Protein_Len: 45 Strand: + Start: 17 End: 151 ................ M R G G T A P N P T K G D D A E A I L Y P E A A P E K P S I D I R L I L L Y T Y F H R R T . attcaatggataatggattaggggaggtacagcccctaatccgacgaaaggcgacgacgcagaggctatactttatcctgaggcggcaccggaaaaaccttcaattgacataaggcttattcttctatatacttattttcataggaggactt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 88 Start: 104 End: 133 .......................................................................................................ATTGACATAAGGCTTATTCTTCTATATACT................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attcaatggataatggattaggggaggtacagcccctaatccgacgaaaggcgacgacgcagaggctatactttatcctgaggcggcaccggaaaaaccttcaattgacataaggcttattcttctatatacttattttcataggaggactt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TDE2737:TDE2738|TDE2737:TDE2738:hypothetical protein:oligoendopeptidase F, putative:->->:2792751..2792902 152 attcaatggataatggattaggggaggtacagcccctaatccgacgaaaggcgacgacgcagaggctatactttatcctgaggcggcaccggaaaaaccttcaattgacataaggcttattcttctatatacttattttcataggaggactt Intra-Species Hit: Count: 1 Min: 1 Max: 152 Len: 152 Subject: NC_002967_TDE2737_TDE2738|hypothetical protein:oligoendopeptidase F, putative|POSITIVE:POSITIVE|[2792751,2792902]|152 HSP 1 e-value: 3.0E-81 bit: 301.0 Len: 152 Query Start:1 Query End:152 Subject Strand: POSITIVE Subject Start: 1 Subject End: 152 attcaatggataatggattaggggaggtacagcccctaatccgacgaaaggcgacgacgcagaggctatactttatcctgaggcggcaccggaaaaaccttcaattgacataaggcttattcttctatatacttattttcataggaggactt attcaatggataatggattaggggaggtacagcccctaatccgacgaaaggcgacgacgcagaggctatactttatcctgaggcggcaccggaaaaaccttcaattgacataaggcttattcttctatatacttattttcataggaggactt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUCAAUGGAUAAUGGAUUAGGGGAGGUACAGCCCCUAAUCCGACGAAAGGCGACGACGCAGAGGCUAUACUUUAUCCUGAGGCGGCACCGGAAAAACCUUCAAUUGACAUAAGGCUUAUUCUUCUAUAUACUUAUUUUCAUAGGAGGACUU .((((..((((((.((((((((((........))))))))))........(((....)))............)))))))))).((....))......(((((...(((.(((((...(((......)))..)))))..)))..))))).... (-39.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGUCCUCCUAUGAAAAUAAGUAUAUAGAAGAAUAAGCCUUAUGUCAAUUGAAGGUUUUUCCGGUGCCGCCUCAGGAUAAAGUAUAGCCUCUGCGUCGUCGCCUUUCGUCGGAUUAGGGGCUGUACCUCCCCUAAUCCAUUAUCCAUUGAAU ..........(((((...............(((.(((((((..........)))))))))).((((.((.(.((((.............)))).).)).)))).))))).((((((((((........)))))))))).............. (-34.62) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 433 67 26 151 113 NC_002967:TDE2103|5end_glycosyl_transferase,_group_2_family_protein_2128393..2128623_NEGATIVE 409 86 42 200 147 NC_002967:TDE0995|5end_hypothetical_protein_1022576..1022806_NEGATIVE 377 60 14 102 42 NC_002967:TDE1982|5end_hypothetical_protein_1992984..1993214_NEGATIVE 376 68 21 217 139 NC_002967:TDE2309|5end_sigma-54_dependent_transcriptional_regulator,_putative_2348099..2348329_NEGATIVE 361 47 2 206 150 NC_002967:TDE1364|5end_valyl-tRNA_synthetase_1404763..1404993_POSITIVE 354 99 51 82 21 NC_002967:TDE1679|5end_ATP_synthase_subunit_K_1731049..1731279_NEGATIVE 354 86 55 33 4 NC_002967:TDE0864|5end_phosphohexose_mutase_family_protein_885244..885474_POSITIVE 345 100 57 188 140 NC_002967:TDE2397|5end_hypothetical_protein_2425564..2425794_NEGATIVE 337 66 21 46 7 NC_002967:TDE0364|5end_ABC_transporter,_ATP-binding_protein_411605..411835_NEGATIVE 334 129 81 189 135 NC_002967:TDE1497|5end_hypothetical_protein_1543208..1543438_POSITIVE 332 90 41 220 168 NC_002967:TDE2042|5end_hypothetical_protein_2072179..2072409_NEGATIVE 330 88 41 155 97 NC_002967:TDE2526|5end_hypothetical_protein_2555034..2555264_NEGATIVE 328 91 51 74 33 NC_002967:TDE0353|5end_lipase/acylhydrolase,_GDSL_family_398338..398568_NEGATIVE 326 87 42 188 118 NC_002967:TDE1199|5end_cell_division_protein_FtsL,_putative_1231341..1231571_POSITIVE 321 60 12 118 64 NC_002967:TDE2134|5end_hypothetical_protein_2164447..2164677_POSITIVE 320 87 49 162 122 NC_002967:TDE2383|5end_cobalamin_5'-phosphate_synthase_2412826..2413056_POSITIVE 316 66 26 219 168 NC_002967:TDE1846|5end_hypothetical_protein_1875426..1875656_NEGATIVE 313 86 41 231 168 NC_002967:TDE1590|5end_aminotransferase,_DegT/DnrJ/EryC1/StrS_family_1637516..1637746_POSITIVE 312 65 30 135 94 NC_002967:TDE2480|5end_heat_shock_protein_90_2505288..2505518_NEGATIVE 312 87 41 49 8 NC_002967:TDE2028|5end_OmpA_family_protein_2052923..2053153_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 399 67 21 140 94 NC_002967:TDE2136|3end_hypothetical_protein_2165390..2165540_NEGATIVE 381 86 41 82 25 NC_002967:TDE1239|3end_hypothetical_protein_1271217..1271367_NEGATIVE 356 85 41 81 29 NC_002967:TDE2292|3end_2C-methyl-D-erythritol_2,4-cyclodiphosphate_synthase_2329383..2329533_POSITIVE 353 88 51 72 35 NC_002967:TDE1529|3end_hypothetical_protein_1575067..1575217_POSITIVE 348 77 41 136 101 NC_002967:TDE1848|3end_hypothetical_protein_1875897..1876047_NEGATIVE 329 42 14 76 47 NC_002967:TDE2737|3end_hypothetical_protein_2792690..2792840_POSITIVE 324 67 29 130 74 NC_002967:TDE1584|3end_lipoprotein,_putative_1632854..1633004_POSITIVE 323 66 26 79 41 NC_002967:TDE2724|3end_hypothetical_protein_2782282..2782432_POSITIVE 321 69 22 75 22 NC_002967:TDE0823|3end_(3R)-hydroxymyristoyl-(acyl-carrier-protein)_dehydratase,_putative_845252..845402_POSITIVE 321 67 26 136 77 NC_002967:TDE0616|3end_hypothetical_protein_650079..650229_POSITIVE 319 67 21 136 82 NC_002967:TDE0742|3end_hypothetical_protein_778450..778600_NEGATIVE 312 65 30 71 30 NC_002967:TDE2481|3end_hypothetical_protein_2505352..2505502_NEGATIVE 312 87 41 44 3 NC_002967:TDE2027|3end_hypothetical_protein_2052998..2053148_POSITIVE 306 43 3 63 34 NC_002967:TDE2337|3end_aminopeptidase_2374604..2374754_POSITIVE 304 98 51 107 51 NC_002967:TDE2642|3end_hypothetical_protein_2682111..2682261_NEGATIVE 301 87 50 108 70 NC_002967:TDE2372|3end_hypothetical_protein_2405199..2405349_POSITIVE 300 91 51 52 2 NC_002967:TDE1887|3end_TPR_domain_protein_1908492..1908642_NEGATIVE 299 120 71 91 52 NC_002967:TDE1998|3end_phosphohexose_mutase_family_protein_2017143..2017293_POSITIVE 298 70 21 109 52 NC_002967:TDE2325|3end_hypothetical_protein_2361177..2361327_POSITIVE 295 70 21 67 9 NC_002967:TDE0967|3end_hypothetical_protein_992529..992679_POSITIVE