Origin IGS: taacagtgagcgtatttaaatgtgtaccgcaaagcctttctaaccaaaaaatgatgcaaaatctccaagcttattagtgtacataatacaaaagattgtaggtcaagc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gcttgacctacaatcttttgtattatgtacactaataagcttggagattttgcatcattttttggttagaaaggctttgcggtacacatttaaatacgctcactgtta Mask Tandem Repeat Region ================================================ taacagtgagcgtatttaaatgtgtaccgcaaagcctttctaaccaaaaaatgatgcaaaatctccaagcttattagtgtacataatacaaaagattgtaggtcaagc Find is-nt database================================================ Query_seq: TDE2481:TDE2482|TDE2481:TDE2482:hypothetical protein:hypothetical protein:<-->:2505568..2505675 108 taacagtgagcgtatttaaatgtgtaccgcaaagcctttctaaccaaaaaatgatgcaaaatctccaagcttattagtgtacataatacaaaagattgtaggtcaagc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE2481:TDE2482|TDE2481:TDE2482:hypothetical protein:hypothetical protein:<-->:2505568..2505675 108 taacagtgagcgtatttaaatgtgtaccgcaaagcctttctaaccaaaaaatgatgcaaaatctccaagcttattagtgtacataatacaaaagattgtaggtcaagc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE2481:TDE2482|TDE2481:TDE2482:hypothetical protein:hypothetical protein:<-->:2505568..2505675 108 taacagtgagcgtatttaaatgtgtaccgcaaagcctttctaaccaaaaaatgatgcaaaatctccaagcttattagtgtacataatacaaaagattgtaggtcaagc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacagtgagcgtatttaaatgtgtaccgcaaagcctttctaaccaaaaaatgatgcaaaatctccaagcttattagtgtacataatacaaaagattgtaggtcaagc Predict ORF larger than 30AA ================================================ taacagtgagcgtatttaaatgtgtaccgcaaagcctttctaaccaaaaaatgatgcaaaatctccaagcttattagtgtacataatacaaaagattgtaggtcaagc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacagtgagcgtatttaaatgtgtaccgcaaagcctttctaaccaaaaaatgatgcaaaatctccaagcttattagtgtacataatacaaaagattgtaggtcaagc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TDE2481:TDE2482|TDE2481:TDE2482:hypothetical protein:hypothetical protein:<-->:2505568..2505675 108 taacagtgagcgtatttaaatgtgtaccgcaaagcctttctaaccaaaaaatgatgcaaaatctccaagcttattagtgtacataatacaaaagattgtaggtcaagc Intra-Species Hit: Count: 1 Min: 1 Max: 108 Len: 108 Subject: NC_002967_TDE2481_TDE2482|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[2505568,2505675]|108 HSP 1 e-value: 4.0E-55 bit: 214.0 Len: 108 Query Start:1 Query End:108 Subject Strand: POSITIVE Subject Start: 1 Subject End: 108 taacagtgagcgtatttaaatgtgtaccgcaaagcctttctaaccaaaaaatgatgcaaaatctccaagcttattagtgtacataatacaaaagattgtaggtcaagc taacagtgagcgtatttaaatgtgtaccgcaaagcctttctaaccaaaaaatgatgcaaaatctccaagcttattagtgtacataatacaaaagattgtaggtcaagc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACAGUGAGCGUAUUUAAAUGUGUACCGCAAAGCCUUUCUAACCAAAAAAUGAUGCAAAAUCUCCAAGCUUAUUAGUGUACAUAAUACAAAAGAUUGUAGGUCAAGC ......(((.(.........((((((((...((((.(((......)))....(((.....))).....))))....).))))))).(((((....)))))).)))... (-11.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCUUGACCUACAAUCUUUUGUAUUAUGUACACUAAUAAGCUUGGAGAUUUUGCAUCAUUUUUUGGUUAGAAAGGCUUUGCGGUACACAUUUAAAUACGCUCACUGUUA ((......(((((....)))))...(((((......((((((.....(((((.((((.....)))))))))))))))....)))))...........))......... (-15.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 866 70 21 161 112 NC_002967:TDE2481|5end_hypothetical_protein_2505477..2505707_NEGATIVE 266 57 27 35 10 NC_002967:TDE1917|5end_cytidylyltransferase_domain_protein_1937394..1937624_NEGATIVE 264 56 27 226 200 NC_002967:TDE1719|5end_transcriptional_regulator,_MarR_family_1774029..1774259_POSITIVE 250 41 4 154 102 NC_002967:TDE1816|5end_hypothetical_protein_1851673..1851903_NEGATIVE 242 69 27 124 85 NC_002967:TDE2181|5end_PAP2_family_protein_2213035..2213265_NEGATIVE 242 46 5 199 153 NC_002967:TDE0882|5end_hypothetical_protein_901449..901679_POSITIVE 230 40 2 69 29 NC_002967:TDE1440|5end_glucose-1-phosphate_thymidylyltransferase_1485353..1485583_POSITIVE 225 57 20 231 194 NC_002967:TDE1299|5end_hypothetical_protein_1336613..1336843_NEGATIVE 222 97 56 188 157 NC_002967:TDE0772|5end_50S_ribosomal_protein_L22_809084..809314_POSITIVE 221 90 63 59 38 NC_002967:TDE1505|5end_hypothetical_protein_1553614..1553844_POSITIVE 221 100 51 137 83 NC_002967:TDE0499|5end_hypothetical_protein_553497..553727_NEGATIVE 216 67 25 102 53 NC_002967:TDE2770|5end_flagellar_hook-length_control_protein_FliK_2824417..2824647_NEGATIVE 216 46 4 72 26 NC_002967:TDE0685|5end_oxidoreductase,_short_chain_dehydrogenase/reductase_family_722620..722850_NEGATIVE 214 68 22 102 46 NC_002967:TDE1541|5end_metallo-beta-lactamase_family_protein_1584898..1585128_POSITIVE 213 66 21 229 185 NC_002967:TDE2359|5end_DAK2_domain_protein_2394782..2395012_NEGATIVE 213 35 4 157 116 NC_002967:TDE1808|5end_hypothetical_protein_1845049..1845279_NEGATIVE 213 70 27 152 103 NC_002967:TDE0998|5end_hypothetical_protein_1025202..1025432_POSITIVE 213 37 7 60 21 NC_002967:TDE0389|5end_(R)-2-hydroxyglutaryl-CoA_dehydratase,_beta_subunit,_putative_440008..440238_POSITIVE 212 34 4 32 10 NC_002967:TDE1905|5end_hypothetical_protein_1923877..1924107_NEGATIVE 211 70 24 109 54 NC_002967:TDE1144|5end_hypothetical_protein_1182855..1183085_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 266 57 27 38 13 NC_002967:TDE1918|3end_hypothetical_protein_1937391..1937541_NEGATIVE 250 41 4 61 9 NC_002967:TDE1817|3end_hypothetical_protein_1851766..1851916_NEGATIVE 248 70 22 55 17 NC_002967:TDE2647|3end_lipoyltransferase_and_lipoate-protein_ligase_family_protein_2686002..2686152_NEGATIVE 245 70 21 91 35 NC_002967:TDE2571|3end_hypothetical_protein_2597922..2598072_NEGATIVE 242 69 27 124 85 NC_002967:TDE2182|3end_hypothetical_protein_2213035..2213185_NEGATIVE 240 50 6 129 82 NC_002967:TDE2299|3end_apolipoprotein_N-acyltransferase_2339937..2340087_POSITIVE 236 45 3 129 75 NC_002967:TDE2210|3end_hypothetical_protein_2249394..2249544_NEGATIVE 227 80 50 38 10 NC_002967:TDE0157|3end_KHG/KDPG_family_aldolase/carbohydrate_kinase,_PfkB_family_185711..185861_POSITIVE 221 100 51 70 16 NC_002967:TDE0500|3end_hypothetical_protein_553564..553714_NEGATIVE 214 68 22 72 16 NC_002967:TDE1540|3end_hypothetical_protein_1584948..1585098_POSITIVE 211 70 24 96 41 NC_002967:TDE1145|3end_hypothetical_protein_1182868..1183018_NEGATIVE 210 70 23 142 97 NC_002967:TDE0322|3end_regulatory_protein,_P-II_family_355106..355256_NEGATIVE 208 68 23 74 32 NC_002967:TDE1750|3end_hypothetical_protein_1803439..1803589_NEGATIVE 199 38 4 114 73 NC_002967:TDE0330|3end_CRISPR-associated_SAG0897_family_protein_366998..367148_POSITIVE 197 92 55 53 10 NC_002967:TDE0921|3end_ABC_transporter,_ATP-binding/permease_protein_940118..940268_NEGATIVE 195 49 5 79 32 NC_002967:TDE2729|3end_hypothetical_protein_2785756..2785906_NEGATIVE 195 43 8 59 18 NC_002967:TDE1369|3end_excinuclease_ABC_subunit_B_1409804..1409954_NEGATIVE 195 48 1 84 48 NC_002967:TDE0578|3end_hypothetical_protein_609677..609827_POSITIVE 195 92 52 102 61 NC_002967:TDE0369|3end_type_I_restriction-modification_system,_M_subunit_418356..418506_NEGATIVE 193 42 2 89 54 NC_002967:TDE2357|3end_iron_compound_ABC_transporter,_permease_protein_2393101..2393251_POSITIVE