Origin IGS: agagaatcgtcctcatcaatactcatatcttcaatctttgtatttaaaaagctgtttataaactcgtctatgtctccatccattacagcctgaatgtttcccgtttcatactttgtcctgtgatcttttaccattgtgtaaggctgaaaaacataggaccttatctgattgccccaagaaataccttttttttcggctgcaaacttcatgttctctttttctttttgttcttcataataattataaagccttgattttagaacattcatagccgtagccctattgctgatttggctcctttgagtttggcaggcaacaactattccggttggaatatgggtaatacgtacggctgagtcggttttgtttacgtgttggcctccggcccctccggctcggtatgtgtcgatacgcaagtcttcagggcgtatatcgacttctatcgtatcatcaaggacaggaaaagcaaagaccgatgtaaaagatgtatgcctccttccgttggaatcaaaagggctgatacgcaccagcctatgaatccccgtttcgctttttaaaaggccgaaggcaaattcgcctgaaatctgaaatgttgcagactttattccgccctcggcttcaaccacatctatggtttccgttttaaagcctctggactcgcaccagcgcaaatacatgcgcaccagcataccggcccaatcgcaggcctcagttccccctgcccctgcatgaaccgtaaggtaggcatcgtttttatcgacctcacctgaaaggaggctcaatatacttaattttttatatttttcgtaaaccgagttataatttgagccgatttcttcagacagttcttcattgccggattcttccgaaagctccatcaaaacttccaaatcggaaacctcatccattagctcctgccaaggataaattctgtttttaagggcctttatctcgcccataattttttcggcttttttactgtcattccaaaaatcgggagctaaggttatagcttctttttcggctatttttgctttaaccgcctcggggtcaaagacgcccccaaatattagagatatcgttttttaaggcttctaaatttgatctatagtcttccataaggt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 accttatggaagactatagatcaaatttagaagccttaaaaaacgatatctctaatatttgggggcgtctttgaccccgaggcggttaaagcaaaaatagccgaaaaagaagctataaccttagctcccgatttttggaatgacagtaaaaaagccgaaaaaattatgggcgagataaaggcccttaaaaacagaatttatccttggcaggagctaatggatgaggtttccgatttggaagttttgatggagctttcggaagaatccggcaatgaagaactgtctgaagaaatcggctcaaattataactcggtttacgaaaaatataaaaaattaagtatattgagcctcctttcaggtgaggtcgataaaaacgatgcctaccttacggttcatgcaggggcagggggaactgaggcctgcgattgggccggtatgctggtgcgcatgtatttgcgctggtgcgagtccagaggctttaaaacggaaaccatagatgtggttgaagccgagggcggaataaagtctgcaacatttcagatttcaggcgaatttgccttcggccttttaaaaagcgaaacggggattcataggctggtgcgtatcagcccttttgattccaacggaaggaggcatacatcttttacatcggtctttgcttttcctgtccttgatgatacgatagaagtcgatatacgccctgaagacttgcgtatcgacacataccgagccggaggggccggaggccaacacgtaaacaaaaccgactcagccgtacgtattacccatattccaaccggaatagttgttgcctgccaaactcaaaggagccaaatcagcaatagggctacggctatgaatgttctaaaatcaaggctttataattattatgaagaacaaaaagaaaaagagaacatgaagtttgcagccgaaaaaaaaggtatttcttggggcaatcagataaggtcctatgtttttcagccttacacaatggtaaaagatcacaggacaaagtatgaaacgggaaacattcaggctgtaatggatggagacatagacgagtttataaacagctttttaaatacaaagattgaagatatgagtattgatgaggacgattctct Mask Tandem Repeat Region ================================================ agagaatcgtcctcatcaatactcatatcttcaatctttgtatttaaaaagctgtttataaactcgtctatgtctccatccattacagcctgaatgtttcccgtttcatactttgtcctgtgatcttttaccattgtgtaaggctgaaaaacataggaccttatctgattgccccaagaaataccttttttttcggctgcaaacttcatgttctctttttctttttgttcttcataataattataaagccttgattttagaacattcatagccgtagccctattgctgatttggctcctttgagtttggcaggcaacaactattccggttggaatatgggtaatacgtacggctgagtcggttttgtttacgtgttggcctccggcccctccggctcggtatgtgtcgatacgcaagtcttcagggcgtatatcgacttctatcgtatcatcaaggacaggaaaagcaaagaccgatgtaaaagatgtatgcctccttccgttggaatcaaaagggctgatacgcaccagcctatgaatccccgtttcgctttttaaaaggccgaaggcaaattcgcctgaaatctgaaatgttgcagactttattccgccctcggcttcaaccacatctatggtttccgttttaaagcctctggactcgcaccagcgcaaatacatgcgcaccagcataccggcccaatcgcaggcctcagttccccctgcccctgcatgaaccgtaaggtaggcatcgtttttatcgacctcacctgaaaggaggctcaatatacttaattttttatatttttcgtaaaccgagttataatttgagccgatttcttcagacagttcttcattgccggattcttccgaaagctccatcaaaacttccaaatcggaaacctcatccattagctcctgccaaggataaattctgtttttaagggcctttatctcgcccataattttttcggcttttttactgtcattccaaaaatcgggagctaaggttatagcttctttttcggctatttttgctttaaccgcctcggggtcaaagacgcccccaaatattagagatatcgttttttaaggcttctaaatttgatctatagtcttccataaggt Find is-nt database================================================ Query_seq: TDE2045:TDE2047|TDE2045:TDE2047:hypothetical protein:hypothetical protein:<-<-:2075024..2076147 1124 agagaatcgtcctcatcaatactcatatcttcaatctttgtatttaaaaagctgtttataaactcgtctatgtctccatccattacagcctgaatgtttcccgtttcatactttgtcctgtgatcttttaccattgtgtaaggctgaaaaacataggaccttatctgattgccccaagaaataccttttttttcggctgcaaacttcatgttctctttttctttttgttcttcataataattataaagccttgattttagaacattcatagccgtagccctattgctgatttggctcctttgagtttggcaggcaacaactattccggttggaatatgggtaatacgtacggctgagtcggttttgtttacgtgttggcctccggcccctccggctcggtatgtgtcgatacgcaagtcttcagggcgtatatcgacttctatcgtatcatcaaggacaggaaaagcaaagaccgatgtaaaagatgtatgcctccttccgttggaatcaaaagggctgatacgcaccagcctatgaatccccgtttcgctttttaaaaggccgaaggcaaattcgcctgaaatctgaaatgttgcagactttattccgccctcggcttcaaccacatctatggtttccgttttaaagcctctggactcgcaccagcgcaaatacatgcgcaccagcataccggcccaatcgcaggcctcagttccccctgcccctgcatgaaccgtaaggtaggcatcgtttttatcgacctcacctgaaaggaggctcaatatacttaattttttatatttttcgtaaaccgagttataatttgagccgatttcttcagacagttcttcattgccggattcttccgaaagctccatcaaaacttccaaatcggaaacctcatccattagctcctgccaaggataaattctgtttttaagggcctttatctcgcccataattttttcggcttttttactgtcattccaaaaatcgggagctaaggttatagcttctttttcggctatttttgctttaaccgcctcggggtcaaagacgcccccaaatattagagatatcgttttttaaggcttctaaatttgatctatagtcttccataaggt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE2045:TDE2047|TDE2045:TDE2047:hypothetical protein:hypothetical protein:<-<-:2075024..2076147 1124 agagaatcgtcctcatcaatactcatatcttcaatctttgtatttaaaaagctgtttataaactcgtctatgtctccatccattacagcctgaatgtttcccgtttcatactttgtcctgtgatcttttaccattgtgtaaggctgaaaaacataggaccttatctgattgccccaagaaataccttttttttcggctgcaaacttcatgttctctttttctttttgttcttcataataattataaagccttgattttagaacattcatagccgtagccctattgctgatttggctcctttgagtttggcaggcaacaactattccggttggaatatgggtaatacgtacggctgagtcggttttgtttacgtgttggcctccggcccctccggctcggtatgtgtcgatacgcaagtcttcagggcgtatatcgacttctatcgtatcatcaaggacaggaaaagcaaagaccgatgtaaaagatgtatgcctccttccgttggaatcaaaagggctgatacgcaccagcctatgaatccccgtttcgctttttaaaaggccgaaggcaaattcgcctgaaatctgaaatgttgcagactttattccgccctcggcttcaaccacatctatggtttccgttttaaagcctctggactcgcaccagcgcaaatacatgcgcaccagcataccggcccaatcgcaggcctcagttccccctgcccctgcatgaaccgtaaggtaggcatcgtttttatcgacctcacctgaaaggaggctcaatatacttaattttttatatttttcgtaaaccgagttataatttgagccgatttcttcagacagttcttcattgccggattcttccgaaagctccatcaaaacttccaaatcggaaacctcatccattagctcctgccaaggataaattctgtttttaagggcctttatctcgcccataattttttcggcttttttactgtcattccaaaaatcgggagctaaggttatagcttctttttcggctatttttgctttaaccgcctcggggtcaaagacgcccccaaatattagagatatcgttttttaaggcttctaaatttgatctatagtcttccataaggt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 3 Min: 45 Max: 998 Len: 954 Subject: gi|89109670|ref|AP_003450.1| peptide chain release factor RF-2 [Escherichia coli W3110] HSP 1 e-value: 5.0E-77 bit: 285.0 Len: 954 Query Start:45 Query End:998 Subject Strand: null Subject Start: 44 Subject End: 361 ............................................ P D F W N D S K K A E K I M G E I K A L K N R I Y P W Q E L M D E V S D L E V L M X X X X X X X X X X X X X X X X X N Y N S V Y E X X X X X X X X X X X S G E V D K N D A Y L T V H A G A G G T E A C D W A G M L V R M Y L R W C E S R G F K T E T I D V V E A E - G G I K S A T F Q I S G E F A F G L L K S E T G I H R L V R I S P F D S N G R R H T S F T S V F A F P V L D D T I E V D I R P E D L R I D T Y R A G G A G G Q H V N K T D S A V R I T H I P T G I V V A C Q T Q R S Q I S N R A T A M N V L K S R L X X X X X X X X X X X N M K F A A E K K G I S W G N Q I R S Y V F Q P Y T M V K D H R T K Y E T G N I Q A V M D G D I D E F I N S F L .............................................................................................................................. ............................................ P D V W N E P E R A Q A L G K E R S S L E A V V D T L D Q M K Q G L E D V S G L L E L A V E A D D E E T F N E A V A E L D A L E E K L A Q L E F R R M F S G E Y D S A D C Y L D I Q A G S G G T E A Q D W A S M L E R M Y L R W A E S R G F K T E I I E E S E G E V A G I K S V T I K I S G D Y A Y G W L R T E T G V H R L V R K S P F D S G G R R H T S F S S A F V Y P E V D D D I D I E I N P A D L R I D V Y R T S G A G G Q H V N R T E S A V R I T H I P T G I V T Q C Q N D R S Q H K N K D Q A M K Q M K A K L Y E L E M Q K K N A E K Q A M E D N K S D I G W G S Q I R S Y V L D D - S R I K D L R T G V E T R N T Q A V L D G S L D Q F I E A S L .............................................................................................................................. Subject: gi|89108056|ref|AP_001836.1| peptide chain release factor RF-1 [Escherichia coli W3110] HSP 1 e-value: 4.0E-44 bit: 175.0 Len: 714 Query Start:45 Query End:758 Subject Strand: null Subject Start: 107 Subject End: 345 ............................................ D K N D A Y L T V H A G A G G T E A C D W A G M L V R M Y L R W C E S R G F K T E T I D V V E A E - G G I K S A T F Q I S G E F A F G L L K S E T G I H R L V R I S P F D S N G R R H T S F T S V F A F P V L D D T I E V D I R P E D L R I D T Y R A G G A G G Q H V N K T D S A V R I T H I P T G I V V A C Q T Q R S Q I S N R A T A M N V L K S R L X X X X X X X X X X X N M K F A A E K K G I - S W G N Q I R S Y V F Q P Y T M V K D H R T K Y E T G N I Q A V M D G D I D E F I N S F L .............................................................................................................................................................................................................................................................................................................................................................................. ............................................ D E R N A F L E V R A G T G G D E A A L F A G D L F R M Y S R Y A E A R R W R V E I M S A S E G E H G G Y K E I I A K I S G D G V Y G R L K F E S G G H R V Q R V P A T E S Q G R I H T S A C T V A V M P E L P D A E L P D I N P A D L R I D T F R S S G A G G Q H V N T T D S A I R I T H L P T G I V V E C Q D E R S Q H K N K A K A L S V L G A R I H A A E M A K R Q Q A E A S T R R N L L G S G D R S D R N R T Y N F - P Q G R V T D H R I N L T L Y R L D E V M E G K L D M L I E P I I .............................................................................................................................................................................................................................................................................................................................................................................. Subject: gi|89107109|ref|AP_000889.1| predicted peptide chain release factor [Escherichia coli W3110] HSP 1 e-value: 1.0E-12 bit: 71.2 Len: 360 Query Start:246 Query End:605 Subject Strand: null Subject Start: 13 Subject End: 127 ..................................................................................................................................................................................................................................................... I K S A T F Q I S G E F A F G L L K S E T G I H R L V R I S P F D S N G R R H T S F T S V F A F P V L D D T I E V D I R P E D L R I D T Y R A G G A G G Q H V N K T D S A V R I T H I P T G I V V A C Q T Q R S Q I S N R A T A M N V L K S R L ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ..................................................................................................................................................................................................................................................... L R S A L V S L D G D N A W A L S E S W C G T I Q W I C P S P Y R P H H G R K N W F L G I G R F - - - - - T A D E Q E Q S D A I R Y E T L R S S G P G G Q H V N K T D S A V R A T H L A S G I S V K V Q S E R S Q H A N K R L A R L L I A W K L ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Find nr database================================================ Query_seq: TDE2045:TDE2047|TDE2045:TDE2047:hypothetical protein:hypothetical protein:<-<-:2075024..2076147 1124 agagaatcgtcctcatcaatactcatatcttcaatctttgtattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagctaaggttatagcttctttttcggctatttttgctttaaccgcctcggggtcaaagacgcccccaaatattagagatatcgttttttaaggcttctaaatttgatctatagtcttccataaggt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agagaatcgtcctcatcaatactcatatcttcaatctttgtattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagctaaggttatagcttctttttcggctatttttgctttaaccgcctcggggtcaaagacgcccccaaatattagagatatcgttttttaaggcttctaaatttgatctatagtcttccataaggt Predict ORF larger than 30AA ================================================ Protein_Len: 58 Strand: + Start: 78 End: 251 ............................................................................. M H Y S L N V S R F I L C P V I F Y H C V R L K N I G P Y L I A P R N T F F F G C K L H V L F F F L F F I I I I K P ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 52 Strand: + Start: 113 End: 268 ................................................................................................................ M S C D L L P L C K A E K H R T L S D C P K K Y L F F R L Q T S C S L F L F V L H N N Y K A L I L E H S ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 30 Strand: + Start: 264 End: 353 ....................................................................................................................................................................................................................................................................... M H S R S P I A D L A P L S L A G N N Y S G W N M G N T Y G ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 42 Strand: + Start: 353 End: 478 ................................................................................................................................................................................................................................................................................................................................................................ M S R F C L R V G L R P L R L G M C R Y A S L Q G V Y R L L S Y H Q G Q E K Q R P M ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 322 End: 534 ................................................................................................................................................................................................................................................................................................................................. M P V G I W V I R T A E S V L F T C W P P A P P A R Y V S I R K S S G R I S T S I V S S R T G K A K T D V K D V C L L P .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 372 End: 554 ................................................................................................................................................................................................................................................................................................................................................................................... M L A S G P S G S V C V D T Q V F R A Y I D F Y R I I K D R K S K D R C K R C M P P S V G I K R A D T H Q P M N P R F A .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 42 Strand: + Start: 604 End: 729 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M P P S A S T T S M V S V L K P L D S H Q R K Y M R T S I P A Q S Q A S V P P A P A ........................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 585 End: 767 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M K C C R L Y S A L G F N H I Y G F R F K A S G L A P A Q I H A H Q H T G P I A G L S S P C P C M N R K V G I V F I D L ..................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: + Start: 832 End: 924 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M S S D S S S L P D S S E S S I K T S K S E T S S I S S C Q G ........................................................................................................................................................................................................ Protein_Len: 57 Strand: + Start: 767 End: 937 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M K G G S I Y L I F Y I F R K P S Y N L S R F L Q T V L H C R I L P K A P S K L P N R K P H P L A P A K D K F C F ........................................................................................................................................................................................... Protein_Len: 53 Strand: + Start: 928 End: 1086 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M L F L R A F I S P I I F S A F L L S F Q K S G A K V I A S F S A I F A L T A S G S K T P P N I R D I V F ...................................... Protein_Len: 45 Strand: - Start: 632 End: 766 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... P K R K L A E P S A G A C I C A C W C V P G I A P R L E G Q G Q M F R L T P M T K I S R M ...................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: - Start: 443 End: 535 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................... R I M L S L F L L S R H L L H I G G E T P I L L A S V C W G M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 40 Strand: - Start: 283 End: 402 .......................................................................................................................................................................................................................................................................................... Q Q N P E K S N P L C C S N R N S Y P Y Y T R S L R N Q K R T P R R G R R S P M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 3 End: 1079 .. E D M L E Q W P Y I R N K L A K I E G M I K E A K K S D N W F D P A L T I A E K E A I K A K V A E P D F V G G F I L S M ............................................. agagaatcgtcctcatcaatactcatatcttcaatctttgtattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagctaaggttatagcttctttttcggctatttttgctttaaccgcctcggggtcaaagacgcccccaaatattagagatatcgttttttaaggcttctaaatttgatctatagtcttccataaggt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 87 Start: 794 End: 823 .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTTATATTTTTCGTAAACCGAGTTATAAT............................................................................................................................................................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agagaatcgtcctcatcaatactcatatcttcaatctttgtattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagctaaggttatagcttctttttcggctatttttgctttaaccgcctcggggtcaaagacgcccccaaatattagagatatcgttttttaaggcttctaaatttgatctatagtcttccataaggt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 68 HP_score: -4.3 Tail_Score: -4.69464 Start: 997 End: 1016 Full_Region: TCATTCCAAAAATCG GGAGCTA AGGTTA TAGCTTC TTTTTCGGCTATTTT ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GGAGCTAAGGTTATAGCTTC............................................................................................................ Find igs database================================================ Query_seq: TDE2045:TDE2047|TDE2045:TDE2047:hypothetical protein:hypothetical protein:<-<-:2075024..2076147 1124 agagaatcgtcctcatcaatactcatatcttcaatctttgtattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagctaaggttatagcttctttttcggctatttttgctttaaccgcctcggggtcaaagacgcccccaaatattagagatatcgttttttaaggcttctaaatttgatctatagtcttccataaggt Intra-Species Hit: Count: 1 Min: 1 Max: 1124 Len: 1124 Subject: NC_002967_TDE2045_TDE2047|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[2075024,2076147]|1124 HSP 1 e-value: 9.0E-65 bit: 250.0 Len: 126 Query Start:999 Query End:1124 Subject Strand: POSITIVE Subject Start: 999 Subject End: 1124 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agctaaggttatagcttctttttcggctatttttgctttaaccgcctcggggtcaaagacgcccccaaatattagagatatcgttttttaaggcttctaaatttgatctatagtcttccataaggt ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agctaaggttatagcttctttttcggctatttttgctttaaccgcctcggggtcaaagacgcccccaaatattagagatatcgttttttaaggcttctaaatttgatctatagtcttccataaggt HSP 2 e-value: 8.0E-16 bit: 87.7 Len: 44 Query Start:1 Query End:44 Subject Strand: POSITIVE Subject Start: 1 Subject End: 44 agagaatcgtcctcatcaatactcatatcttcaatctttgtatt........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ agagaatcgtcctcatcaatactcatatcttcaatctttgtatt........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAGAAUCGUCCUCAUCAAUACUCAUAUCUUCAAUCUUUGUAUUUAAAAAGCUGUUUAUAAACUCGUCUAUGUCUCCAUCCAUUACAGCCUGAAUGUUUCCCGUUUCAUACUUUGUCCUGUGAUCUUUUACCAUUGUGUAAGGCUGAAAAACAUAGGACCUUAUCUGAUUGCCCCAAGAAAUACCUUUUUUUUCGGCUGCAAACUUCAUGUUCUCUUUUUCUUUUUGUUCUUCAUAAUAAUUAUAAAGCCUUGAUUUUAGAACAUUCAUAGCCGUAGCCCUAUUGCUGAUUUGGCUCCUUUGAGUUUGGCAGGCAACAACUAUUCCGGUUGGAAUAUGGGUAAUACGUACGGCUGAGUCGGUUUUGUUUACGUGUUGGCCUCCGGCCCCUCCGGCUCGGUAUGUGUCGAUACGCAAGUCUUCAGGGCGUAUAUCGACUUCUAUCGUAUCAUCAAGGACAGGAAAAGCAAAGACCGAUGUAAAAGAUGUAUGCCUCCUUCCGUUGGAAUCAAAAGGGCUGAUACGCACCAGCCUAUGAAUCCCCGUUUCGCUUUUUAAAAGGCCGAAGGCAAAUUCGCCUGAAAUCUGAAAUGUUGCAGACUUUAUUCCGCCCUCGGCUUCAACCACAUCUAUGGUUUCCGUUUUAAAGCCUCUGGACUCGCACCAGCGCAAAUACAUGCGCACCAGCAUACCGGCCCAAUCGCAGGCCUCAGUUCCCCCUGCCCCUGCAUGAACCGUAAGGUAGGCAUCGUUUUUAUCGACCUCACCUGAAAGGAGGCUCAAUAUACUUAAUUUUUUAUAUUUUUCGUAAACCGAGUUAUAAUUUGAGCCGAUUUCUUCAGACAGUUCUUCAUUGCCGGAUUCUUCCGAAAGCUCCAUCAAAACUUCCAAAUCGGAAACCUCAUCCAUUAGCUCCUGCCAAGGAUAAAUUCUGUUUUUAAGGGCCUUUAUCUCGCCCAUAAUUUUUUCGGCUUUUUUACUGUCAUUCCAAAAAUCGGGAGCUAAGGUUAUAGCUUCUUUUUCGGCUAUUUUUGCUUUAACCGCCUCGGGGUCAAAGACGCCCCCAAAUAUUAGAGAUAUCGUUUUUUAAGGCUUCUAAAUUUGAUCUAUAGUCUUCCAUAAGGU .(((.......)))..........((((((..(((.((((.......(((((...((((((.((((.....((((((........(((((.(((((((((..((.(((......((((((((...(((((((.........)).)))))..))))))))......)))..)).....))))))........)))))))).....((((.((..(((((((.(((((((((((.((............(((((((...((((((...(.((((((((((((..(((((.....(((((....))))).....)))))....((((((....)))))).))))......)))))))).)...))))))....((((((((((.((((((.....))).)))...).))))))))).......)))))))......(((......)))....)).))))))))))).)).))))).(((((...((((((.((((((((((.((..(((.(((...((((((........)))))).))).)))....(((...........(((((.((((......))))..........(((((((((((((((....(((...)))...(((((......))))).......)))))..))))...........(((((......)))))..))))))..))))).....(((((...(((......)))..)))))..))).)).)))).))))))))))))))))).....)).)))).))))))..((((((..........))))))..........))))))))))....((((((........((((((........((((((.......((((((...........((((.....)))).....((((.((.((....)).)))))).......))))))..((((.........))))........))))))......))))))............((((((((......))))))))...))))))......))))).........(((((.......))))))))).)))..))))))........((((((................))))))......... (-285.63) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACCUUAUGGAAGACUAUAGAUCAAAUUUAGAAGCCUUAAAAAACGAUAUCUCUAAUAUUUGGGGGCGUCUUUGACCCCGAGGCGGUUAAAGCAAAAAUAGCCGAAAAAGAAGCUAUAACCUUAGCUCCCGAUUUUUGGAAUGACAGUAAAAAAGCCGAAAAAAUUAUGGGCGAGAUAAAGGCCCUUAAAAACAGAAUUUAUCCUUGGCAGGAGCUAAUGGAUGAGGUUUCCGAUUUGGAAGUUUUGAUGGAGCUUUCGGAAGAAUCCGGCAAUGAAGAACUGUCUGAAGAAAUCGGCUCAAAUUAUAACUCGGUUUACGAAAAAUAUAAAAAAUUAAGUAUAUUGAGCCUCCUUUCAGGUGAGGUCGAUAAAAACGAUGCCUACCUUACGGUUCAUGCAGGGGCAGGGGGAACUGAGGCCUGCGAUUGGGCCGGUAUGCUGGUGCGCAUGUAUUUGCGCUGGUGCGAGUCCAGAGGCUUUAAAACGGAAACCAUAGAUGUGGUUGAAGCCGAGGGCGGAAUAAAGUCUGCAACAUUUCAGAUUUCAGGCGAAUUUGCCUUCGGCCUUUUAAAAAGCGAAACGGGGAUUCAUAGGCUGGUGCGUAUCAGCCCUUUUGAUUCCAACGGAAGGAGGCAUACAUCUUUUACAUCGGUCUUUGCUUUUCCUGUCCUUGAUGAUACGAUAGAAGUCGAUAUACGCCCUGAAGACUUGCGUAUCGACACAUACCGAGCCGGAGGGGCCGGAGGCCAACACGUAAACAAAACCGACUCAGCCGUACGUAUUACCCAUAUUCCAACCGGAAUAGUUGUUGCCUGCCAAACUCAAAGGAGCCAAAUCAGCAAUAGGGCUACGGCUAUGAAUGUUCUAAAAUCAAGGCUUUAUAAUUAUUAUGAAGAACAAAAAGAAAAAGAGAACAUGAAGUUUGCAGCCGAAAAAAAAGGUAUUUCUUGGGGCAAUCAGAUAAGGUCCUAUGUUUUUCAGCCUUACACAAUGGUAAAAGAUCACAGGACAAAGUAUGAAACGGGAAACAUUCAGGCUGUAAUGGAUGGAGACAUAGACGAGUUUAUAAACAGCUUUUUAAAUACAAAGAUUGAAGAUAUGAGUAUUGAUGAGGACGAUUCUCU .((((((.((..(((....(((..........(((((((.....(((((((((((...((((((((..(((((((((....).)))))))).....(((((..........))))).......))))))))...))))...((((((......((((......(((.((((.........)))).)))........((((((((((((....))))).))))))).(((((((.....((((((.....)))))))))))))....))))........))))))........((((((((((((.....((((((((((...((((((..........))))))..((((((....((((...((((.......)))))))).((((.((((((...((((((((((..((.(.((((((..(((..((((.(((((((.((((((((......)))))......((((((....(((((........((((((....))))))...((((((((((((.(...(((((.(((......).)).))))).).)))))))))))).......))))).......))))))....)))...))))))).))))..)))...((..(....)..)).................)))))).).))..))))))))))..((((((....(((((...............))))).))))))..........)))))).))))...)))))).....)))).....))))))...(((((((.(.....(((.((((((....))))))..((((((.((.....(((....)))......)))))))))))))))))))....(((((((....((....(((((((.....)))))))........)).....)))))))..)))))).))))))......)))))))..)))))))..((((.....(((((.((.((((..(((.........)))..)))).)).)))))...((((......(....)....((((((((((..(((....)))......))).....))))))).....)))).....)))).)))...))).)).)))))).......... (-329.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 916 120 71 211 162 NC_002967:TDE2045|5end_hypothetical_protein_2074933..2075163_NEGATIVE 500 736 692 99 37 NC_002967:TDE2209|5end_CarB_family_protein_2247589..2247819_POSITIVE 432 416 374 72 29 NC_002967:TDE0693|5end_phosphomethylpyrimidine_kinase_729942..730172_POSITIVE 408 396 353 114 64 NC_002967:TDE2473|5end_30S_ribosomal_protein_S21_2496298..2496528_POSITIVE 402 428 383 70 21 NC_002967:TDE0473|5end_heavy_metal_translocating_P-type_ATPase_528621..528851_POSITIVE 388 418 371 224 177 NC_002967:TDE2640|5end_hypothetical_protein_2680034..2680264_POSITIVE 387 430 381 204 158 NC_002967:TDE1985|5end_hypothetical_protein_2000329..2000559_NEGATIVE 386 715 675 157 113 NC_002967:TDE2516|5end_hypothetical_protein_2542021..2542251_POSITIVE 383 397 351 162 112 NC_002967:TDE2596|5end_hypothetical_protein_2632039..2632269_NEGATIVE 383 727 693 113 79 NC_002967:TDE2205|5end_enoate_reductase,_putative_2244755..2244985_NEGATIVE 381 739 692 93 43 NC_002967:TDE2485|5end_hypothetical_protein_2509123..2509353_NEGATIVE 380 399 353 143 80 NC_002967:TDE1926|5end_hypothetical_protein_1942495..1942725_POSITIVE 379 398 351 226 168 NC_002967:TDE0573|5end_hypothetical_protein_604681..604911_POSITIVE 378 397 351 198 130 NC_002967:TDE2583|5end_hypothetical_protein_2616322..2616552_POSITIVE 375 414 371 56 10 NC_002967:TDE0852|5end_50S_ribosomal_protein_L13_875019..875249_POSITIVE 373 733 692 77 37 NC_002967:TDE0071|5end_peptidase,_U32_family_78602..78832_POSITIVE 371 408 362 200 137 NC_002967:TDE2714|5end_hypothetical_protein_2772618..2772848_NEGATIVE 371 330 289 137 90 NC_002967:TDE1066|5end_hypothetical_protein_1094544..1094774_NEGATIVE 370 429 381 153 95 NC_002967:TDE1139|5end_hypothetical_protein_1168615..1168845_NEGATIVE 369 396 351 172 123 NC_002967:TDE2429|5end_hypothetical_protein_2458037..2458267_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 446 729 684 142 81 NC_002967:TDE1450|3end_MutS2_family_protein_1497856..1498006_POSITIVE 442 728 688 67 27 NC_002967:TDE1131|3end_hypothetical_protein_1163531..1163681_POSITIVE 422 727 682 139 93 NC_002967:TDE2527|3end_hypothetical_protein_2555449..2555599_NEGATIVE 390 429 383 125 84 NC_002967:TDE0833|3end_lipoprotein,_putative_854097..854247_POSITIVE 380 399 351 44 1 NC_002967:TDE2162|3end_NOL1/NOP2/sun_family_protein_2195560..2195710_POSITIVE 371 330 289 137 90 NC_002967:TDE1067|3end_oligopeptide/dipeptide_ABC_transporter,_ATP-binding_protein_1094544..1094694_NEGATIVE 369 410 363 144 94 NC_002967:TDE1847|3end_hypothetical_protein_1875845..1875995_POSITIVE 367 397 351 112 52 NC_002967:TDE1637|3end_DNA_polymerase_I_1688962..1689112_POSITIVE 362 400 351 61 3 NC_002967:TDE1347|3end_hypothetical_protein_1388032..1388182_POSITIVE 361 728 682 78 3 NC_002967:TDE1396|3end_hypothetical_protein_1436127..1436277_POSITIVE 361 739 692 146 105 NC_002967:TDE0602|3end_3-oxoacyl-(acyl-carrier-protein)_synthase_III_639836..639986_NEGATIVE 360 749 701 150 84 NC_002967:TDE1665|3end_hypothetical_protein_1718908..1719058_NEGATIVE 358 729 681 101 56 NC_002967:TDE0157|3end_KHG/KDPG_family_aldolase/carbohydrate_kinase,_PfkB_family_185711..185861_POSITIVE 357 707 663 85 40 NC_002967:TDE2250|3end_hypothetical_protein_2289409..2289559_POSITIVE 354 620 575 64 3 NC_002967:TDE2573|3end_glucose-6-phosphate_isomerase_2600459..2600609_POSITIVE 354 414 375 52 6 NC_002967:TDE1998|3end_phosphohexose_mutase_family_protein_2017143..2017293_POSITIVE 353 399 351 65 16 NC_002967:TDE2104|3end_hypothetical_protein_2128525..2128675_NEGATIVE 350 726 692 91 50 NC_002967:TDE2582|3end_GGDEF_domain_protein_2616164..2616314_POSITIVE 347 533 492 80 32 NC_002967:TDE2426|3end_transcription_antitermination_protein_NusG_2455242..2455392_NEGATIVE 346 729 683 142 85 NC_002967:TDE2631|3end_peptide_ABC_transporter,_permease_protein,_putative_2672733..2672883_POSITIVE