Origin IGS: tattttttatcttcggttcttgccttttcttcttcctgcatctcaaacagagtcttcgtagttttacaaagagcatttacatcctttgtttttaaaagatttattgctgcctgcagctgctcatcatcttcatctataacgggactttcgtgagagcggtagtattcaacttttacaagacttaaaatcagctcatgtcttacattgtattctttaccaagctttttagaaaaatccatcatctcattttttgaaatagaaggcttgctccttgtaaagtttgcaattttagaagtctttaaaagctcaagtacgctcttttcttcatcgggtgtaaaactaggccttttaacttccaagtcgggaagaattccctgcttatttatatttgccccgctaggagaatagtatcttgctatggtaagcttcacaagctccttttcgctcaactgaacgacactttgcaccaagcccttgccgtaagagcgggttcctacaagataagcccttttatgatcctttaaggcacctgccacaatttcggaagcgcttgcagaaccttcatttatgagaacaactatgggcatatctttaggaagcttttttacgaatcggcggacattataagtttcgcttgtgttgcgggctcttccctttgtcgaaaccacaacacccgattctaaaaagatactcgctacatcaatagagcttgtaatcaaaccgcccggattattctttaaatcaaagatcagctttgtacagccttcttcttgtaatttttctatagcctcggttattcgttttgccgaattaggattaaactctattaaacggatataggcaatatcctttccgatttttgtatactttatggtaggaacttcgattatggctcgtgtcagtgtaagatcaaactttagattttttcctctaagaacttttattgtaacctgagtaccttctttcccccgaagcatatttaaaacatcctcctgggtaatctcttcggtttttactccgtcgatttcgataatatagtctcccggttgaagtccggccttccaacccggagttccttcaatagggcttgcaacttcgacataggcaggacgctcaggagtagatacattcggttttgtaatatgaacacctatacctccaaaaacaccggttgtagtatcctccaaatcatgacccgccgtcgtatccttaaaaatataagaggtataagggtcttgaagagaattaagcatcccttccatagcccccttatataaaacggcaggatctatttcatctacataattttcttgaagaagtttgtacacggattcaagatatcgtaaatttacatcggcattacctgcatcggattccgaattagatgttgcaggagccctctgcggcataaaaaaaatagccgccaacagaagagttaaaaagataatagtatttatccaagttattcttttattcatctttctattttgaactatattgtaaatttgtcaatagggtaaagaatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aattctttaccctattgacaaatttacaatatagttcaaaatagaaagatgaataaaagaataacttggataaatactattatctttttaactcttctgttggcggctattttttttatgccgcagagggctcctgcaacatctaattcggaatccgatgcaggtaatgccgatgtaaatttacgatatcttgaatccgtgtacaaacttcttcaagaaaattatgtagatgaaatagatcctgccgttttatataagggggctatggaagggatgcttaattctcttcaagacccttatacctcttatatttttaaggatacgacggcgggtcatgatttggaggatactacaaccggtgtttttggaggtataggtgttcatattacaaaaccgaatgtatctactcctgagcgtcctgcctatgtcgaagttgcaagccctattgaaggaactccgggttggaaggccggacttcaaccgggagactatattatcgaaatcgacggagtaaaaaccgaagagattacccaggaggatgttttaaatatgcttcgggggaaagaaggtactcaggttacaataaaagttcttagaggaaaaaatctaaagtttgatcttacactgacacgagccataatcgaagttcctaccataaagtatacaaaaatcggaaaggatattgcctatatccgtttaatagagtttaatcctaattcggcaaaacgaataaccgaggctatagaaaaattacaagaagaaggctgtacaaagctgatctttgatttaaagaataatccgggcggtttgattacaagctctattgatgtagcgagtatctttttagaatcgggtgttgtggtttcgacaaagggaagagcccgcaacacaagcgaaacttataatgtccgccgattcgtaaaaaagcttcctaaagatatgcccatagttgttctcataaatgaaggttctgcaagcgcttccgaaattgtggcaggtgccttaaaggatcataaaagggcttatcttgtaggaacccgctcttacggcaagggcttggtgcaaagtgtcgttcagttgagcgaaaaggagcttgtgaagcttaccatagcaagatactattctcctagcggggcaaatataaataagcagggaattcttcccgacttggaagttaaaaggcctagttttacacccgatgaagaaaagagcgtacttgagcttttaaagacttctaaaattgcaaactttacaaggagcaagccttctatttcaaaaaatgagatgatggatttttctaaaaagcttggtaaagaatacaatgtaagacatgagctgattttaagtcttgtaaaagttgaatactaccgctctcacgaaagtcccgttatagatgaagatgatgagcagctgcaggcagcaataaatcttttaaaaacaaaggatgtaaatgctctttgtaaaactacgaagactctgtttgagatgcaggaagaagaaaaggcaagaaccgaagataaaaaata Mask Tandem Repeat Region ================================================ tattttttatcttcggttcttgccttttcttcttcctgcatctcaaacagagtcttcgtagttttacaaagagcatttacatcctttgtttttaaaagatttattgctgcctgcagctgctcatcatcttcatctataacgggactttcgtgagagcggtagtattcaacttttacaagacttaaaatcagctcatgtcttacattgtattctttaccaagctttttagaaaaatccatcatctcattttttgaaatagaaggcttgctccttgtaaagtttgcaattttagaagtctttaaaagctcaagtacgctcttttcttcatcgggtgtaaaactaggccttttaacttccaagtcgggaagaattccctgcttatttatatttgccccgctaggagaatagtatcttgctatggtaagcttcacaagctccttttcgctcaactgaacgacactttgcaccaagcccttgccgtaagagcgggttcctacaagataagcccttttatgatcctttaaggcacctgccacaatttcggaagcgcttgcagaaccttcatttatgagaacaactatgggcatatctttaggaagcttttttacgaatcggcggacattataagtttcgcttgtgttgcgggctcttccctttgtcgaaaccacaacacccgattctaaaaagatactcgctacatcaatagagcttgtaatcaaaccgcccggattattctttaaatcaaagatcagctttgtacagccttcttcttgtaatttttctatagcctcggttattcgttttgccgaattaggattaaactctattaaacggatataggcaatatcctttccgatttttgtatactttatggtaggaacttcgattatggctcgtgtcagtgtaagatcaaactttagattttttcctctaagaacttttattgtaacctgagtaccttctttcccccgaagcatatttaaaacatcctcctgggtaatctcttcggtttttactccgtcgatttcgataatatagtctcccggttgaagtccggccttccaacccggagttccttcaatagggcttgcaacttcgacataggcaggacgctcaggagtagatacattcggttttgtaatatgaacacctatacctccaaaaacaccggttgtagtatcctccaaatcatgacccgccgtcgtatccttaaaaatataagaggtataagggtcttgaagagaattaagcatcccttccatagcccccttatataaaacggcaggatctatttcatctacataattttcttgaagaagtttgtacacggattcaagatatcgtaaatttacatcggcattacctgcatcggattccgaattagatgttgcaggagccctctgcggcataaaaaaaatagccgccaacagaagagttaaaaagataatagtatttatccaagttattcttttattcatctttctattttgaactatattgtaaatttgtcaatagggtaaagaatt Find is-nt database================================================ Query_seq: TDE1877:TDE1878|TDE1877:TDE1878:conserved hypothetical protein TIGR00046:hydrolase, haloacid dehalogenase-like family:<-<-:1898524..1900049 1526 tattttttatcttcggttcttgccttttcttcttcctgcatctcaaacagagtcttcgtagttttacaaagagcatttacatcctttgtttttaaaagatttattgctgcctgcagctgctcatcatcttcatctataacgggactttcgtgagagcggtagtattcaacttttacaagacttaaaatcagctcatgtcttacattgtattctttaccaagctttttagaaaaatccatcatctcattttttgaaatagaaggcttgctccttgtaaagtttgcaattttagaagtctttaaaagctcaagtacgctcttttcttcatcgggtgtaaaactaggccttttaacttccaagtcgggaagaattccctgcttatttatatttgccccgctaggagaatagtatcttgctatggtaagcttcacaagctccttttcgctcaactgaacgacactttgcaccaagcccttgccgtaagagcgggttcctacaagataagcccttttatgatcctttaaggcacctgccacaatttcggaagcgcttgcagaaccttcatttatgagaacaactatgggcatatctttaggaagcttttttacgaatcggcggacattataagtttcgcttgtgttgcgggctcttccctttgtcgaaaccacaacacccgattctaaaaagatactcgctacatcaatagagcttgtaatcaaaccgcccggattattctttaaatcaaagatcagctttgtacagccttcttcttgtaatttttctatagcctcggttattcgttttgccgaattaggattaaactctattaaacggatataggcaatatcctttccgatttttgtatactttatggtaggaacttcgattatggctcgtgtcagtgtaagatcaaactttagattttttcctctaagaacttttattgtaacctgagtaccttctttcccccgaagcatatttaaaacatcctcctgggtaatctcttcggtttttactccgtcgatttcgataatatagtctcccggttgaagtccggccttccaacccggagttccttcaatagggcttgcaacttcgacataggcaggacgctcaggagtagatacattcggttttgtaatatgaacacctatacctccaaaaacaccggttgtagtatcctccaaatcatgacccgccgtcgtatccttaaaaatataagaggtataagggtcttgaagagaattaagcatcccttccatagcccccttatataaaacggcaggatctatttcatctacataattttcttgaagaagtttgtacacggattcaagatatcgtaaatttacatcggcattacctgcatcggattccgaattagatgttgcaggagccctctgcggcataaaaaaaatagccgccaacagaagagttaaaaagataatagtatttatccaagttattcttttattcatctttctattttgaactatattgtaaatttgtcaatagggtaaagaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE1877:TDE1878|TDE1877:TDE1878:conserved hypothetical protein TIGR00046:hydrolase, haloacid dehalogenase-like family:<-<-:1898524..1900049 1526 tattttttatcttcggttcttgccttttcttcttcctgcatctcaaacagagtcttcgtagttttacaaagagcatttacatcctttgtttttaaaagatttattgctgcctgcagctgctcatcatcttcatctataacgggactttcgtgagagcggtagtattcaacttttacaagacttaaaatcagctcatgtcttacattgtattctttaccaagctttttagaaaaatccatcatctcattttttgaaatagaaggcttgctccttgtaaagtttgcaattttagaagtctttaaaagctcaagtacgctcttttcttcatcgggtgtaaaactaggccttttaacttccaagtcgggaagaattccctgcttatttatatttgccccgctaggagaatagtatcttgctatggtaagcttcacaagctccttttcgctcaactgaacgacactttgcaccaagcccttgccgtaagagcgggttcctacaagataagcccttttatgatcctttaaggcacctgccacaatttcggaagcgcttgcagaaccttcatttatgagaacaactatgggcatatctttaggaagcttttttacgaatcggcggacattataagtttcgcttgtgttgcgggctcttccctttgtcgaaaccacaacacccgattctaaaaagatactcgctacatcaatagagcttgtaatcaaaccgcccggattattctttaaatcaaagatcagctttgtacagccttcttcttgtaatttttctatagcctcggttattcgttttgccgaattaggattaaactctattaaacggatataggcaatatcctttccgatttttgtatactttatggtaggaacttcgattatggctcgtgtcagtgtaagatcaaactttagattttttcctctaagaacttttattgtaacctgagtaccttctttcccccgaagcatatttaaaacatcctcctgggtaatctcttcggtttttactccgtcgatttcgataatatagtctcccggttgaagtccggccttccaacccggagttccttcaatagggcttgcaacttcgacataggcaggacgctcaggagtagatacattcggttttgtaatatgaacacctatacctccaaaaacaccggttgtagtatcctccaaatcatgacccgccgtcgtatccttaaaaatataagaggtataagggtcttgaagagaattaagcatcccttccatagcccccttatataaaacggcaggatctatttcatctacataattttcttgaagaagtttgtacacggattcaagatatcgtaaatttacatcggcattacctgcatcggattccgaattagatgttgcaggagccctctgcggcataaaaaaaatagccgccaacagaagagttaaaaagataatagtatttatccaagttattcttttattcatctttctattttgaactatattgtaaatttgtcaatagggtaaagaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 198 Max: 1343 Len: 1146 Subject: gi|89108670|ref|AP_002450.1| carboxy-terminal protease for penicillin-binding protein 3 [Escherichia coli W3110] HSP 1 e-value: 7.0E-24 bit: 108.0 Len: 1146 Query Start:198 Query End:1343 Subject Strand: null Subject Start: 194 Subject End: 578 ..................................................................................................................................................................................................... R Y L E S V Y K L L Q E N Y V D E I D P A V L Y K G A M E G M L N S L Q D P Y T S Y I F K D T T A G H D L E D T T T G V F G G I G V H I T K P N V S T P E R P A Y V E V A S P I E G T P G W K A - G L Q P G D Y I I E I D G V K T E E I T Q - - - - - E D V L N M L R G K E G T Q V T I K V L - R G K N L K F - D L T L T R A I I E V P T - - - - I K Y T K I G K D - I A Y I R L I E F N P N S A K R I T E A I E K L Q E E G C T K L I F D L K N N P G G L I T S S I D V A S I F L E S G V V V S T K - - - G R A R N T S E T Y N V R R F V K K L P K D M P I V V L I N E G S A S A S E I V A G A L K D H K R A Y L V G T R S Y G K G L V Q - - - S V V Q L S E K E L - - - - - - - - - V K L T I A R Y Y S P S G A N I N K Q G I L P D L E V K R P S F T P D E E K S V L E L L K T S K I A - N F T R S K P S I S K N E M M D F S K K L G K E Y N V R ....................................................................................................................................................................................... ..................................................................................................................................................................................................... R Y K F A I R R L A Q T N S E D - - - - - - V F S L A M T A F A R E I - D P H T N Y L S P R N T E Q F N T E M S L S - - L E G I G A V L Q M D D - - - - - - - D Y T V I N S M V A G G P A A K S K A I S V G D K I V G V G Q T G K P M V D V I G W R L D D V V A L I K G P K G S K V R L E I L P A G K G T K T R T V T L T R E R I R L E D R A V K M S V K T V G K E K V G V L D I P G F Y V G L T D D V K V Q L Q K L E K Q N V S S V I I D L R S N G G G A L T E A V S L S G L F I P A G P I V Q V R D N N G K V R E D S D T - D G Q V F Y K G - - - - - P L V V L V D R F S A S A S E I F A A A M Q D Y G R A L V V G E P T F G K G T V Q Q Y R S L N R I Y D Q M L R P E W P A L G S V Q Y T I Q K F Y R V N G G S T Q R K G V T P D I I M P - - - - T G N E E T E T G E K F E D N A L P W D S I D A A T Y V K S G D L T A F E P E L L K E H N A R ....................................................................................................................................................................................... Find nr database================================================ Query_seq: TDE1877:TDE1878|TDE1877:TDE1878:conserved hypothetical protein TIGR00046:hydrolase, haloacid dehalogenase-like family:<-<-:1898524..1900049 1526 tattttttatcttcggttcttgccttttcttcttcctgcatctcaaacagagtcttcgtagttttacaaagagcatttacatcctttgtttttaaaagatttattgctgcctgcagctgctcatcatcttcatctataacgggactttcgtgagagcggtagtattcaacttttacaagacttaaaatcagctcatgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaaatttacatcggcattacctgcatcggattccgaattagatgttgcaggagccctctgcggcataaaaaaaatagccgccaacagaagagttaaaaagataatagtatttatccaagttattcttttattcatctttctattttgaactatattgtaaatttgtcaatagggtaaagaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tattttttatcttcggttcttgccttttcttcttcctgcatctcaaacagagtcttcgtagttttacaaagagcatttacatcctttgtttttaaaagatttattgctgcctgcagctgctcatcatcttcatctataacgggactttcgtgagagcggtagtattcaacttttacaagacttaaaatcagctcatgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaaatttacatcggcattacctgcatcggattccgaattagatgttgcaggagccctctgcggcataaaaaaaatagccgccaacagaagagttaaaaagataatagtatttatccaagttattcttttattcatctttctattttgaactatattgtaaatttgtcaatagggtaaagaatt Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 40 End: 150 ....................................... M S N R V F V V L Q R A F T S F V F K R F I A A C S C S S S S S I T G L S ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 187 End: 405 .......................................................................................................................................................................................... M S S C L T L Y S L P S F L E K S I I S F F E I E G L L L V K F A I L E V F K S S S T L F S S S G V K L G L L T S K S G ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 327 End: 521 ...................................................................................................................................................................................................................................................................................................................................... M G C K T R P F N F Q V G K N S L L I Y I C P A R R I V S C Y G K L H K L L F A Q L N D T L H Q A L A V R A G S Y K I S ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 39 Strand: + Start: 564 End: 680 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M Y E N N Y G H I F R K L F Y E S A D I I S F A C V A G S S L C R N H N T R F .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 31 Strand: + Start: 611 End: 703 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M G G H Y K F R L C C G L F P L S K P Q H P I L K R Y S L H Q ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 637 End: 837 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M L R A L P F V E T T T P D S K K I L A T S I E L V I K P P G L F F K S K I S F V Q P S S C N F S I A S V I R F A E L G ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 36 Strand: + Start: 844 End: 951 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M S F P I F V Y F M V G T S I M A R V S V R S N F R F F P L R T F I V T ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 987 End: 1274 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M L L G N L F G F Y S V D F D N I V S R L K S G L P T R S S F N R A C N F D I G R T L R S R Y I R F C N M N T Y T S K N ............................................................................................................................................................................................................................................................ Protein_Len: 36 Strand: + Start: 1274 End: 1381 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M K R Q D L F H L H N F L E E V C T R I Q D I V N L H R H Y L H R I P N ................................................................................................................................................. Protein_Len: 30 Strand: + Start: 1347 End: 1436 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M Y I G I T C I G F R I R C C R S P L R H K K N S R Q Q K S .......................................................................................... Protein_Len: 56 Strand: + Start: 1321 End: 1488 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M Y T D S R Y R K F T S A L P A S D S E L D V A G A L C G I K K I A A N R R V K K I I V F I Q V I L L F I F L F ...................................... Protein_Len: 45 Strand: - Start: 1154 End: 1288 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. V E L F V P Q L I R W I M V R R R I R L F I L P I L T K F L I L C G K W L G R I Y F P L M .............................................................................................................................................................................................................................................. Protein_Len: 45 Strand: - Start: 1016 End: 1150 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E T S K S L I T E R N F D P R G V R L E K L L A Q L K S M P L V S L L L Y M R N Q L I F M ........................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 44 Strand: - Start: 934 End: 1065 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... S S K N Y G S Y R R E G S A Y K F C G G P Y D R R N K S R R N R Y Y L R G T S T R G E M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 3 End: 1505 .. V S E L Y R L N V D A N G A D S E S N S T A P A R Q P M F F I A A L L L T L F I I T N I W T I R K N M K R N Q V I N Y M ..................... tattttttatcttcggttcttgccttttcttcttcctgcatctcaaacagagtcttcgtagttttacaaagagcatttacatcctttgtttttaaaagatttattgctgcctgcagctgctcatcatcttcatctataacgggactttcgtgagagcggtagtattcaacttttacaagacttaaaatcagctcatgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaaatttacatcggcattacctgcatcggattccgaattagatgttgcaggagccctctgcggcataaaaaaaatagccgccaacagaagagttaaaaagataatagtatttatccaagttattcttttattcatctttctattttgaactatattgtaaatttgtcaatagggtaaagaatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tattttttatcttcggttcttgccttttcttcttcctgcatctcaaacagagtcttcgtagttttacaaagagcatttacatcctttgtttttaaaagatttattgctgcctgcagctgctcatcatcttcatctataacgggactttcgtgagagcggtagtattcaacttttacaagacttaaaatcagctcatgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaaatttacatcggcattacctgcatcggattccgaattagatgttgcaggagccctctgcggcataaaaaaaatagccgccaacagaagagttaaaaagataatagtatttatccaagttattcttttattcatctttctattttgaactatattgtaaatttgtcaatagggtaaagaatt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 48 HP_score: -4.3 Tail_Score: -2.73441 Start: 743 End: 757 Full_Region: aagaagaaggctgta caaag ctgat ctttg atttaaagaataatc ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................caaagctgatctttg................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 60 HP_score: -5.2 Tail_Score: -3.82067 Start: 833 End: 849 Full_Region: TAAACTCTATTAAAC GGATAT AGGCA ATATCC TTTCCGATTTTTGTA ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GGATATAGGCAATATCC..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 50 HP_score: -5.2 Tail_Score: -2.86288 Start: 833 End: 849 Full_Region: tacaaaaatcggaaa ggatat tgcct atatcc gtttaatagagttta ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggatattgcctatatcc..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 44 HP_score: -3.6 Tail_Score: -2.50252 Start: 1039 End: 1073 Full_Region: agccctattgaagga actccgggttggaag gccgga cttcaaccgggaga ctatattatcgaaat ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................actccgggttggaaggccggacttcaaccgggaga..................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 64 HP_score: -7.1 Tail_Score: -3.37344 Start: 1040 End: 1072 Full_Region: TTTCGATAATATAGT CTCCCGGTTGAAG TCCGGC CTTCCAACCCGGAG TTCCTTCAATAGGGC ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CTCCCGGTTGAAGTCCGGCCTTCCAACCCGGAG...................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: TDE1877:TDE1878|TDE1877:TDE1878:conserved hypothetical protein TIGR00046:hydrolase, haloacid dehalogenase-like family:<-<-:1898524..1900049 1526 tattttttatcttcggttcttgccttttcttcttcctgcatctcaaacagagtcttcgtagttttacaaagagcatttacatcctttgtttttaaaagatttattgctgcctgcagctgctcatcatcttcatctataacgggactttcgtgagagcggtagtattcaacttttacaagacttaaaatcagctcatgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaaatttacatcggcattacctgcatcggattccgaattagatgttgcaggagccctctgcggcataaaaaaaatagccgccaacagaagagttaaaaagataatagtatttatccaagttattcttttattcatctttctattttgaactatattgtaaatttgtcaatagggtaaagaatt Intra-Species Hit: Count: 1 Min: 1 Max: 1526 Len: 1526 Subject: NC_002967_TDE1877_TDE1878|conserved hypothetical protein TIGR00046:hydrolase, haloacid dehalogenase-like family|NEGATIVE:NEGATIVE|[1898524,1900049]|1526 HSP 1 e-value: 1.0E-106 bit: 389.0 Len: 196 Query Start:1 Query End:196 Subject Strand: POSITIVE Subject Start: 1 Subject End: 196 tattttttatcttcggttcttgccttttcttcttcctgcatctcaaacagagtcttcgtagttttacaaagagcatttacatcctttgtttttaaaagatttattgctgcctgcagctgctcatcatcttcatctataacgggactttcgtgagagcggtagtattcaacttttacaagacttaaaatcagctcat.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. tattttttatcttcggttcttgccttttcttcttcctgcatctcaaacagagtcttcgtagttttacaaagagcatttacatcctttgtttttaaaagatttattgctgcctgcagctgctcatcatcttcatctataacgggactttcgtgagagcggtagtattcaacttttacaagacttaaaatcagctcat.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 3.0E-84 bit: 315.0 Len: 183 Query Start:1344 Query End:1526 Subject Strand: POSITIVE Subject Start: 1344 Subject End: 1526 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................taaatttacatcggcattacctgcatcggattccgaattagatgttgcaggagccctctgcggcatnnnnnnnntagccgccaacagaagagttaaaaagataatagtatttatccaagttattcttttattcatctttctattttgaactatattgtaaatttgtcaatagggtaaagaatt ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................taaatttacatcggcattacctgcatcggattccgaattagatgttgcaggagccctctgcggcataaaaaaaatagccgccaacagaagagttaaaaagataatagtatttatccaagttattcttttattcatctttctattttgaactatattgtaaatttgtcaatagggtaaagaatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUUUUUUAUCUUCGGUUCUUGCCUUUUCUUCUUCCUGCAUCUCAAACAGAGUCUUCGUAGUUUUACAAAGAGCAUUUACAUCCUUUGUUUUUAAAAGAUUUAUUGCUGCCUGCAGCUGCUCAUCAUCUUCAUCUAUAACGGGACUUUCGUGAGAGCGGUAGUAUUCAACUUUUACAAGACUUAAAAUCAGCUCAUGUCUUACAUUGUAUUCUUUACCAAGCUUUUUAGAAAAAUCCAUCAUCUCAUUUUUUGAAAUAGAAGGCUUGCUCCUUGUAAAGUUUGCAAUUUUAGAAGUCUUUAAAAGCUCAAGUACGCUCUUUUCUUCAUCGGGUGUAAAACUAGGCCUUUUAACUUCCAAGUCGGGAAGAAUUCCCUGCUUAUUUAUAUUUGCCCCGCUAGGAGAAUAGUAUCUUGCUAUGGUAAGCUUCACAAGCUCCUUUUCGCUCAACUGAACGACACUUUGCACCAAGCCCUUGCCGUAAGAGCGGGUUCCUACAAGAUAAGCCCUUUUAUGAUCCUUUAAGGCACCUGCCACAAUUUCGGAAGCGCUUGCAGAACCUUCAUUUAUGAGAACAACUAUGGGCAUAUCUUUAGGAAGCUUUUUUACGAAUCGGCGGACAUUAUAAGUUUCGCUUGUGUUGCGGGCUCUUCCCUUUGUCGAAACCACAACACCCGAUUCUAAAAAGAUACUCGCUACAUCAAUAGAGCUUGUAAUCAAACCGCCCGGAUUAUUCUUUAAAUCAAAGAUCAGCUUUGUACAGCCUUCUUCUUGUAAUUUUUCUAUAGCCUCGGUUAUUCGUUUUGCCGAAUUAGGAUUAAACUCUAUUAAACGGAUAUAGGCAAUAUCCUUUCCGAUUUUUGUAUACUUUAUGGUAGGAACUUCGAUUAUGGCUCGUGUCAGUGUAAGAUCAAACUUUAGAUUUUUUCCUCUAAGAACUUUUAUUGUAACCUGAGUACCUUCUUUCCCCCGAAGCAUAUUUAAAACAUCCUCCUGGGUAAUCUCUUCGGUUUUUACUCCGUCGAUUUCGAUAAUAUAGUCUCCCGGUUGAAGUCCGGCCUUCCAACCCGGAGUUCCUUCAAUAGGGCUUGCAACUUCGACAUAGGCAGGACGCUCAGGAGUAGAUACAUUCGGUUUUGUAAUAUGAACACCUAUACCUCCAAAAACACCGGUUGUAGUAUCCUCCAAAUCAUGACCCGCCGUCGUAUCCUUAAAAAUAUAAGAGGUAUAAGGGUCUUGAAGAGAAUUAAGCAUCCCUUCCAUAGCCCCCUUAUAUAAAACGGCAGGAUCUAUUUCAUCUACAUAAUUUUCUUGAAGAAGUUUGUACACGGAUUCAAGAUAUCGUAAAUUUACAUCGGCAUUACCUGCAUCGGAUUCCGAAUUAGAUGUUGCAGGAGCCCUCUGCGGCAUAAAAAAAAUAGCCGCCAACAGAAGAGUUAAAAAGAUAAUAGUAUUUAUCCAAGUUAUUCUUUUAUUCAUCUUUCUAUUUUGAACUAUAUUGUAAAUUUGUCAAUAGGGUAAAGAAUU .(((((((((((((((((((((((...........((((.(((((...(((((((.(((.(.....)...((((.........((((.......)))).......((((....)))).))))................))))))))))..)))))))))..((((..(((((((.(((((..(((((.........(((((((((((..(((((.((((.(((...(((((((((........(((.....)))...(((((((((((..(((((.((((((((..(((((((....)))))))...((((...(((.(((((((...((((.(((((..(((((.......(((((......)))))........)))))......)))))))))..))))))).)))..)))).....)))))))).))))).......(((.((....)).)))......((.((..........((((((((.((.((((((.((((((.((((((((((((........(((....))).........((((..(.....)...))))..))))))))........)))).)))))))))))).))))))))))....(((((..(((((((((((...((((((.(((((.((((......((((.............))))......))))..))))).))).)))..)))))))))))....))))).)).....(((((.....)))))...))...))).)))))))).......))))))))).))).((((......((((..((......))...))))..........((((((.....))))))...))))...............)))))))))...(((.......)))...))))))))))).....((((((........))))))...)))))......(((((((..((((........)))).............(((((((((((((...(....)...)))))).(((((....(((......))).(((.((((((((((((.........)))))....))))))).))).........)))))..))).)))).))))))).((((((....(((((((((...((((......))))....))))...)))))..((((.(((((((.....(((((.....)))))..........(((((((.(((..(((((.(((((......)))))...))))).....)))..))))))).....)).)))))))))...)))))).......))))).))))))).))))..))...))))).))))((((((((((.((((..........((((...)))).....((((((((((.....))))))))))..........)))).....((((((((......(((((......)))))......))))))))..........................)))))))))).....)))))))))))). (-372.18) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUCUUUACCCUAUUGACAAAUUUACAAUAUAGUUCAAAAUAGAAAGAUGAAUAAAAGAAUAACUUGGAUAAAUACUAUUAUCUUUUUAACUCUUCUGUUGGCGGCUAUUUUUUUUAUGCCGCAGAGGGCUCCUGCAACAUCUAAUUCGGAAUCCGAUGCAGGUAAUGCCGAUGUAAAUUUACGAUAUCUUGAAUCCGUGUACAAACUUCUUCAAGAAAAUUAUGUAGAUGAAAUAGAUCCUGCCGUUUUAUAUAAGGGGGCUAUGGAAGGGAUGCUUAAUUCUCUUCAAGACCCUUAUACCUCUUAUAUUUUUAAGGAUACGACGGCGGGUCAUGAUUUGGAGGAUACUACAACCGGUGUUUUUGGAGGUAUAGGUGUUCAUAUUACAAAACCGAAUGUAUCUACUCCUGAGCGUCCUGCCUAUGUCGAAGUUGCAAGCCCUAUUGAAGGAACUCCGGGUUGGAAGGCCGGACUUCAACCGGGAGACUAUAUUAUCGAAAUCGACGGAGUAAAAACCGAAGAGAUUACCCAGGAGGAUGUUUUAAAUAUGCUUCGGGGGAAAGAAGGUACUCAGGUUACAAUAAAAGUUCUUAGAGGAAAAAAUCUAAAGUUUGAUCUUACACUGACACGAGCCAUAAUCGAAGUUCCUACCAUAAAGUAUACAAAAAUCGGAAAGGAUAUUGCCUAUAUCCGUUUAAUAGAGUUUAAUCCUAAUUCGGCAAAACGAAUAACCGAGGCUAUAGAAAAAUUACAAGAAGAAGGCUGUACAAAGCUGAUCUUUGAUUUAAAGAAUAAUCCGGGCGGUUUGAUUACAAGCUCUAUUGAUGUAGCGAGUAUCUUUUUAGAAUCGGGUGUUGUGGUUUCGACAAAGGGAAGAGCCCGCAACACAAGCGAAACUUAUAAUGUCCGCCGAUUCGUAAAAAAGCUUCCUAAAGAUAUGCCCAUAGUUGUUCUCAUAAAUGAAGGUUCUGCAAGCGCUUCCGAAAUUGUGGCAGGUGCCUUAAAGGAUCAUAAAAGGGCUUAUCUUGUAGGAACCCGCUCUUACGGCAAGGGCUUGGUGCAAAGUGUCGUUCAGUUGAGCGAAAAGGAGCUUGUGAAGCUUACCAUAGCAAGAUACUAUUCUCCUAGCGGGGCAAAUAUAAAUAAGCAGGGAAUUCUUCCCGACUUGGAAGUUAAAAGGCCUAGUUUUACACCCGAUGAAGAAAAGAGCGUACUUGAGCUUUUAAAGACUUCUAAAAUUGCAAACUUUACAAGGAGCAAGCCUUCUAUUUCAAAAAAUGAGAUGAUGGAUUUUUCUAAAAAGCUUGGUAAAGAAUACAAUGUAAGACAUGAGCUGAUUUUAAGUCUUGUAAAAGUUGAAUACUACCGCUCUCACGAAAGUCCCGUUAUAGAUGAAGAUGAUGAGCAGCUGCAGGCAGCAAUAAAUCUUUUAAAAACAAAGGAUGUAAAUGCUCUUUGUAAAACUACGAAGACUCUGUUUGAGAUGCAGGAAGAAGAAAAGGCAAGAACCGAAGAUAAAAAAUA ...(((((....(((((.........)))))..((((....(((((((((((((..(((.....)))..))........)))))))))))..((((((....(((((............))))).......(((((((.((((((..((((...((......)).....))))...............(((((((...((......))...)))))))........)))))).....((.(((((((((...((((((((..((...(((((((........))))))).)).))))))))..(((((......)))))....))))))))))).......((((.....((((.((..((((((((((((......(((((((...........((((((((((.(((((..(((.((.((((.((((((((..((....(((.....)))..((((.(((((.(((......)))))))).))))......(((.((((.(((.((((.......((((........(((.((((.((((.....)))).)))).)))..((((((((((((..((((((.(((..(((.....((((..((((.....))))..)))).....)))..((((..((((((((.(.(((...................((((..(((((...((((((.........)))).))...))))).(((((......)))))..))))..........((((((.((((....((((......))))..))))))))))............))).).))))))))...))))..))).))))))))))))))))))....))))(((((((((.(((..(.....)..))).))))))))).................)))).))))))))))......((((((((((((.((((..........(((....))).((((((..(((((((((((......))).))))).)))..))))))......)))).)))))).))))))...)).))).))))).))))..)))))......((((((....))))))..)))))........(((......))).)))))).))))...(((((((((...............(((....(((((......)))))....(((((...)))))...)))..........((((((((.((.((((((((((((((..(((((((((...((((((...((((........(((((((.....))))))).((((....))))....)))).))))))......(((.....)))..)).))))))))))))).)))))))).))...)).)))))).......)))))))))............)))))))((((....)))).......))))))))))))..)).)))).....((((((((....))))))))))))........)))))))))))))........)))).)))))......... (-419.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 915 60 11 151 102 NC_002967:TDE1877|5end_conserved_hypothetical_protein_TIGR00046_1898433..1898663_NEGATIVE 417 1079 1037 132 96 NC_002967:TDE1312|5end_hypothetical_protein_1349088..1349318_POSITIVE 397 1405 1363 222 162 NC_002967:TDE2622|5end_ABC_transporter,_ATP-binding_protein_2663590..2663820_NEGATIVE 376 1210 1166 139 77 NC_002967:TDE1926|5end_hypothetical_protein_1942495..1942725_POSITIVE 374 679 634 223 183 NC_002967:TDE1058|5end_hypothetical_protein_1086366..1086596_NEGATIVE 373 489 448 172 129 NC_002967:TDE2714|5end_hypothetical_protein_2772618..2772848_NEGATIVE 373 1518 1471 140 93 NC_002967:TDE2143|5end_penicillin-binding_protein_2178107..2178337_POSITIVE 370 1087 1044 80 23 NC_002967:TDE0682|5end_preprotein_translocase,_YajC_subunit_721078..721308_NEGATIVE 365 1099 1051 199 147 NC_002967:TDE2777|5end_hypothetical_protein_2828312..2828542_POSITIVE 365 494 455 188 146 NC_002967:TDE0028|5end_ABC_transporter,_ATP-binding_protein,_HlyB_family_35930..36160_NEGATIVE 361 1115 1079 38 1 NC_002967:TDE1042|5end_deoxyuridine_5'triphosphate_nucleotidohydrolase_1070492..1070722_POSITIVE 361 1047 1001 217 172 NC_002967:TDE0691|5end_ABC_transporter,_ATP-binding/permease_protein_728062..728292_POSITIVE 359 1075 1036 89 40 NC_002967:TDE1895|5end_GTP-binding_protein_Era_1914963..1915193_POSITIVE 359 510 461 203 155 NC_002967:TDE0773|5end_30S_ribosomal_protein_S3_809443..809673_POSITIVE 355 1407 1364 70 16 NC_002967:TDE2209|5end_CarB_family_protein_2247589..2247819_POSITIVE 353 1099 1051 64 28 NC_002967:TDE0693|5end_phosphomethylpyrimidine_kinase_729942..730172_POSITIVE 346 1096 1055 217 171 NC_002967:TDE0675|5end_hypothetical_protein_713994..714224_POSITIVE 346 129 81 131 75 NC_002967:TDE0531|5end_hypothetical_protein_574874..575104_POSITIVE 344 1099 1053 158 102 NC_002967:TDE1076|5end_oligopeptide/dipeptide_ABC_transporter,_ATP-binding_protein_1106936..1107166_POSITIVE 344 1087 1044 171 120 NC_002967:TDE0690|5end_tRNA_(5-methylaminomethyl-2-thiouridylate)-methyltransferase_726898..727128_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 374 679 634 91 51 NC_002967:TDE1059|3end_hypothetical_protein_1086498..1086648_NEGATIVE 369 1057 1016 125 66 NC_002967:TDE2265|3end_hypothetical_protein_2302634..2302784_POSITIVE 359 1075 1036 87 38 NC_002967:TDE1894|3end_ferredoxin_1915041..1915191_POSITIVE 354 798 752 133 83 NC_002967:TDE0947|3end_translation_elongation_factor_G,_putative_968714..968864_NEGATIVE 350 657 611 151 100 NC_002967:TDE1989|3end_acyltransferase_family_protein_2005585..2005735_NEGATIVE 346 129 81 60 4 NC_002967:TDE0530|3end_hypothetical_protein_574883..575033_POSITIVE 340 1078 1043 75 35 NC_002967:TDE2606|3end_urocanate_hydratase_2648551..2648701_POSITIVE 339 1406 1362 122 67 NC_002967:TDE1079|3end_DNA-binding_protein/PTS_system,_IIA_component_1111805..1111955_POSITIVE 336 55 19 151 112 NC_002967:TDE0842|3end_cytoplasmic_filament_protein_A_863843..863993_POSITIVE 332 508 467 141 84 NC_002967:TDE0621|3end_MATE_efflux_family_protein_654776..654926_POSITIVE 331 1090 1045 139 88 NC_002967:TDE1105|3end_ribosome-binding_factor_A_1137801..1137951_NEGATIVE 330 1110 1068 151 120 NC_002967:TDE1384|3end_cadmium-translocating_P-type_ATPase_1424776..1424926_POSITIVE 328 448 403 61 4 NC_002967:TDE2551|3end_hypothetical_protein_2576212..2576362_NEGATIVE 327 680 633 123 67 NC_002967:TDE1450|3end_MutS2_family_protein_1497856..1498006_POSITIVE 326 1085 1041 75 21 NC_002967:TDE1982|3end_hypothetical_protein_1992511..1992661_NEGATIVE 326 1135 1098 148 103 NC_002967:TDE0583|3end_hypothetical_protein_621426..621576_NEGATIVE 323 1407 1373 146 106 NC_002967:TDE1892|3end_hypothetical_protein_1912237..1912387_POSITIVE 322 1396 1352 45 2 NC_002967:TDE0740|3end_hypothetical_protein_776204..776354_POSITIVE 321 563 525 133 99 NC_002967:TDE0833|3end_lipoprotein,_putative_854097..854247_POSITIVE 320 1099 1056 70 17 NC_002967:TDE2436|3end_hypothetical_protein_2467446..2467596_POSITIVE