Origin IGS: atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttgacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttagaagtga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tcacttctaacacccgcttcaacctgacattgcggacaagccgcaaatgcaggtcaagcgaatgttagacttagtttgataatcaccagaaaaagaatatttttttacttacatgattaattaatgtgaatatattacgaatatcatatttatgctaaattttattatgtggctatatttattttgaggtaatgat Mask Tandem Repeat Region ================================================ atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttgacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttagaagtga Find is-nt database================================================ Query_seq: TDE1835:TDE1836|TDE1835:TDE1836:hypothetical protein:hypothetical protein:<-<-:1868408..1868603 196 atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttgacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttagaagtga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE1835:TDE1836|TDE1835:TDE1836:hypothetical protein:hypothetical protein:<-<-:1868408..1868603 196 atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttgacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttagaagtga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE1835:TDE1836|TDE1835:TDE1836:hypothetical protein:hypothetical protein:<-<-:1868408..1868603 196 atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttgacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttagaagtga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttgacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttagaagtga Predict ORF larger than 30AA ================================================ atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttgacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttagaagtga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttgacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttagaagtga Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TDE1835:TDE1836|TDE1835:TDE1836:hypothetical protein:hypothetical protein:<-<-:1868408..1868603 196 atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttgacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttagaagtga Intra-Species Hit: Count: 2 Min: 1 Max: 196 Len: 196 Subject: NC_002967_TDE1835_TDE1836|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1868408,1868603]|196 HSP 1 e-value: 9.0E-95 bit: 347.0 Len: 196 Query Start:1 Query End:196 Subject Strand: POSITIVE Subject Start: 1 Subject End: 196 atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtnnnnnnntattctttttctggtgattatcaaactaagtctaacattcgcttgacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttagaagtga atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttgacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttagaagtga Subject: NC_002967_TDE1779_TDE1780|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1822087,1822272]|186 HSP 1 e-value: 5.0E-84 bit: 311.0 Len: 186 Query Start:1 Query End:186 Subject Strand: POSITIVE Subject Start: 1 Subject End: 186 atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtnnnnnnntattctttttctggtgattatcaaactaagtctaacattcgcttgacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtg.......... atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttaacctgcatttgcggctcgtccgcaatgtcaggttgaagcgggtg.......... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCAUUACCUCAAAAUAAAUAUAGCCACAUAAUAAAAUUUAGCAUAAAUAUGAUAUUCGUAAUAUAUUCACAUUAAUUAAUCAUGUAAGUAAAAAAAUAUUCUUUUUCUGGUGAUUAUCAAACUAAGUCUAACAUUCGCUUGACCUGCAUUUGCGGCUUGUCCGCAAUGUCAGGUUGAAGCGGGUGUUAGAAGUGA ...((((((..((((..(((((.((................))....(((((((.....((((........))))....)))))))..........)))))..))))..))))))........((..((((((((((((((((((((((((.((((.....)))))))).)))))).))))))))))))))..)). (-51.39) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCACUUCUAACACCCGCUUCAACCUGACAUUGCGGACAAGCCGCAAAUGCAGGUCAAGCGAAUGUUAGACUUAGUUUGAUAAUCACCAGAAAAAGAAUAUUUUUUUACUUACAUGAUUAAUUAAUGUGAAUAUAUUACGAAUAUCAUAUUUAUGCUAAAUUUUAUUAUGUGGCUAUAUUUAUUUUGAGGUAAUGAU ((((.(((((((..(((((..(((((.((((((((.....)))).))))))))).)))))..)))))))......((((((((((..((.((((((....)))))).))....))))).))))).))))...((((((...((..(((..(((((((.((......)).)))).)))..)))..))..)))))).. (-46.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1033 190 141 180 131 NC_002967:TDE1839|5end_hypothetical_protein_1871221..1871451_NEGATIVE 1033 190 141 205 156 NC_002967:TDE1832|5end_hypothetical_protein_1865921..1866151_NEGATIVE 1033 190 141 203 154 NC_002967:TDE1820|5end_hypothetical_protein_1854692..1854922_NEGATIVE 1010 190 141 220 171 NC_002967:TDE1831|5end_hypothetical_protein_1865032..1865262_NEGATIVE 1010 190 141 187 138 NC_002967:TDE1827|5end_hydrolase,_carbon-nitrogen_family_1860830..1861060_NEGATIVE 1010 190 141 201 152 NC_002967:TDE1823|5end_hypothetical_protein_1856990..1857220_NEGATIVE 1010 190 141 185 136 NC_002967:TDE1817|5end_hypothetical_protein_1852599..1852829_NEGATIVE 1010 190 141 182 133 NC_002967:TDE1811|5end_hypothetical_protein_1847483..1847713_NEGATIVE 1010 190 141 220 171 NC_002967:TDE1797|5end_hypothetical_protein_1836272..1836502_NEGATIVE 1010 190 141 173 124 NC_002967:TDE1795|5end_funZ_protein,_putative_1834673..1834903_NEGATIVE 1010 190 141 191 142 NC_002967:TDE0267|5end_HAM1_protein_299108..299338_NEGATIVE 984 180 131 230 181 NC_002967:TDE1799|5end_hypothetical_protein_1837264..1837494_NEGATIVE 954 190 141 204 156 NC_002967:TDE1796|5end_hypothetical_protein_1835515..1835745_NEGATIVE 933 190 141 180 131 NC_002967:TDE1822|5end_hypothetical_protein_1855922..1856152_NEGATIVE 929 190 141 190 141 NC_002967:TDE1791|5end_hypothetical_protein_1828775..1829005_NEGATIVE 887 180 131 224 175 NC_002967:TDE1790|5end_hypothetical_protein_1828007..1828237_NEGATIVE 883 180 131 224 175 NC_002967:TDE1813|5end_hypothetical_protein_1848249..1848479_NEGATIVE 870 189 141 181 131 NC_002967:TDE1803|5end_hypothetical_protein_1839270..1839500_NEGATIVE 816 190 141 194 144 NC_002967:TDE0262|5end_hypothetical_protein_296300..296530_NEGATIVE 734 190 141 193 143 NC_002967:TDE0279|5end_hypothetical_protein_309782..310012_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1033 190 141 77 28 NC_002967:TDE1840|3end_hypothetical_protein_1871324..1871474_NEGATIVE 1033 190 141 80 31 NC_002967:TDE1821|3end_hypothetical_protein_1854815..1854965_NEGATIVE 1033 190 141 80 31 NC_002967:TDE1810|3end_hypothetical_protein_1845924..1846074_NEGATIVE 1033 190 141 85 36 NC_002967:TDE1808|3end_hypothetical_protein_1844084..1844234_NEGATIVE 1033 190 141 77 28 NC_002967:TDE1800|3end_hypothetical_protein_1837427..1837577_NEGATIVE 1010 190 141 94 45 NC_002967:TDE1843|3end_hypothetical_protein_1872199..1872349_NEGATIVE 1010 190 141 84 35 NC_002967:TDE1837|3end_hypothetical_protein_1869542..1869692_NEGATIVE 1010 190 141 80 31 NC_002967:TDE1832|3end_hypothetical_protein_1865172..1865322_NEGATIVE 1010 190 141 83 34 NC_002967:TDE1828|3end_hypothetical_protein_1860934..1861084_NEGATIVE 1010 190 141 85 36 NC_002967:TDE1812|3end_hypothetical_protein_1847580..1847730_NEGATIVE 1010 190 141 80 31 NC_002967:TDE1796|3end_hypothetical_protein_1834766..1834916_NEGATIVE 1010 190 141 76 27 NC_002967:TDE1787|3end_hypothetical_protein_1826378..1826528_NEGATIVE 1010 190 141 94 45 NC_002967:TDE1780|3end_hypothetical_protein_1822183..1822333_NEGATIVE 1010 190 141 80 31 NC_002967:TDE0268|3end_hypothetical_protein_299219..299369_NEGATIVE 991 190 141 89 40 NC_002967:TDE1806|3end_hypothetical_protein_1843190..1843340_NEGATIVE 983 190 141 94 45 NC_002967:TDE1793|3end_hydrolase,_carbon-nitrogen_family_1829957..1830107_NEGATIVE 982 190 141 83 34 NC_002967:TDE1776|3end_hypothetical_protein_1818414..1818564_NEGATIVE 980 190 141 85 36 NC_002967:TDE1789|3end_hypothetical_protein_1827338..1827488_NEGATIVE 968 190 141 78 29 NC_002967:TDE0097|3end_hypothetical_protein_114801..114951_POSITIVE 959 190 141 111 62 NC_002967:TDE1815|3end_hypothetical_protein_1849458..1849608_NEGATIVE