Origin IGS: actcctctccttcaaatctattctatattttttaagaaataaagcaagtatttcatgcaaagatatttttattttctatggtttccctttgcgcgtgagcgtcattctaacattcgcttaacctgcattgccgcaaaggcaatgtcaggttgaagcgggtg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cacccgcttcaacctgacattgcctttgcggcaatgcaggttaagcgaatgttagaatgacgctcacgcgcaaagggaaaccatagaaaataaaaatatctttgcatgaaatacttgctttatttcttaaaaaatatagaatagatttgaaggagaggagt Mask Tandem Repeat Region ================================================ actcctctccttcaaatctattctatattttttaagaaataaagcaagtatttcatgcaaagatatttttattttctatggtttccctttgcgcgtgagcgtcattctaacattcgcttaacctgcattgccgcaaaggcaatgtcaggttgaagcgggtg Find is-nt database================================================ Query_seq: TDE1826:TDE1827|TDE1826:TDE1827:hypothetical protein:hydrolase, carbon-nitrogen family:<-<-:1859872..1860032 161 actcctctccttcaaatctattctatattttttaagaaataaagcaagtatttcatgcaaagatatttttattttctatggtttccctttgcgcgtgagcgtcattctaacattcgcttaacctgcattgccgcaaaggcaatgtcaggttgaagcgggtg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE1826:TDE1827|TDE1826:TDE1827:hypothetical protein:hydrolase, carbon-nitrogen family:<-<-:1859872..1860032 161 actcctctccttcaaatctattctatattttttaagaaataaagcaagtatttcatgcaaagatatttttattttctatggtttccctttgcgcgtgagcgtcattctaacattcgcttaacctgcattgccgcaaaggcaatgtcaggttgaagcgggtg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE1826:TDE1827|TDE1826:TDE1827:hypothetical protein:hydrolase, carbon-nitrogen family:<-<-:1859872..1860032 161 actcctctccttcaaatctattctatattttttaagaaataaagcaagtatttcatgcaaagatatttttattttctatggtttccctttgcgcgtgagcgtcattctaacattcgcttaacctgcattgccgcaaaggcaatgtcaggttgaagcgggtg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 actcctctccttcaaatctattctatattttttaagaaataaagcaagtatttcatgcaaagatatttttattttctatggtttccctttgcgcgtgagcgtcattctaacattcgcttaacctgcattgccgcaaaggcaatgtcaggttgaagcgggtg Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 20 End: 118 ................... M L Y F L R N K A S I S C K D I F I F Y G F P L R V S V I L T F A ........................................... Protein_Len: 32 Strand: + Start: 55 End: 150 ...................................................... M Q R Y F Y F L W F P F A R E R H S N I R L T C I A A K A M S G ........... actcctctccttcaaatctattctatattttttaagaaataaagcaagtatttcatgcaaagatatttttattttctatggtttccctttgcgcgtgagcgtcattctaacattcgcttaacctgcattgccgcaaaggcaatgtcaggttgaagcgggtg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ actcctctccttcaaatctattctatattttttaagaaataaagcaagtatttcatgcaaagatatttttattttctatggtttccctttgcgcgtgagcgtcattctaacattcgcttaacctgcattgccgcaaaggcaatgtcaggttgaagcgggtg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TDE1826:TDE1827|TDE1826:TDE1827:hypothetical protein:hydrolase, carbon-nitrogen family:<-<-:1859872..1860032 161 actcctctccttcaaatctattctatattttttaagaaataaagcaagtatttcatgcaaagatatttttattttctatggtttccctttgcgcgtgagcgtcattctaacattcgcttaacctgcattgccgcaaaggcaatgtcaggttgaagcgggtg Intra-Species Hit: Count: 1 Min: 1 Max: 161 Len: 161 Subject: NC_002967_TDE1826_TDE1827|hypothetical protein:hydrolase, carbon-nitrogen family|NEGATIVE:NEGATIVE|[1859872,1860032]|161 HSP 1 e-value: 2.0E-86 bit: 319.0 Len: 161 Query Start:1 Query End:161 Subject Strand: POSITIVE Subject Start: 1 Subject End: 161 actcctctccttcaaatctattctatattttttaagaaataaagcaagtatttcatgcaaagatatttttattttctatggtttccctttgcgcgtgagcgtcattctaacattcgcttaacctgcattgccgcaaaggcaatgtcaggttgaagcgggtg actcctctccttcaaatctattctatattttttaagaaataaagcaagtatttcatgcaaagatatttttattttctatggtttccctttgcgcgtgagcgtcattctaacattcgcttaacctgcattgccgcaaaggcaatgtcaggttgaagcgggtg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACUCCUCUCCUUCAAAUCUAUUCUAUAUUUUUUAAGAAAUAAAGCAAGUAUUUCAUGCAAAGAUAUUUUUAUUUUCUAUGGUUUCCCUUUGCGCGUGAGCGUCAUUCUAACAUUCGCUUAACCUGCAUUGCCGCAAAGGCAAUGUCAGGUUGAAGCGGGUG ........((..............((((((((((.((((((.......)))))).)).)))))))).............)).........((((....))))........((((((((((((((((((((((.....))))))).))))))).)))))))) (-46.03) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CACCCGCUUCAACCUGACAUUGCCUUUGCGGCAAUGCAGGUUAAGCGAAUGUUAGAAUGACGCUCACGCGCAAAGGGAAACCAUAGAAAAUAAAAAUAUCUUUGCAUGAAAUACUUGCUUUAUUUCUUAAAAAAUAUAGAAUAGAUUUGAAGGAGAGGAGU ....(((((.((((((.(((((((.....)))))))))))))))))).((((.(((.((.(((....)))))..((....))................)))..))))......(((.(((((..(((...............)))..)))))..))).... (-45.56) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1034 140 91 231 182 NC_002967:TDE1826|5end_hypothetical_protein_1859781..1860011_NEGATIVE 1010 160 111 172 123 NC_002967:TDE0268|5end_hypothetical_protein_300049..300279_NEGATIVE 750 160 111 178 126 NC_002967:TDE1836|5end_hypothetical_protein_1869443..1869673_NEGATIVE 750 160 111 215 163 NC_002967:TDE1831|5end_hypothetical_protein_1865032..1865262_NEGATIVE 750 160 111 180 128 NC_002967:TDE1817|5end_hypothetical_protein_1852599..1852829_NEGATIVE 750 160 111 177 125 NC_002967:TDE1811|5end_hypothetical_protein_1847483..1847713_NEGATIVE 750 160 111 215 163 NC_002967:TDE1797|5end_hypothetical_protein_1836272..1836502_NEGATIVE 750 160 111 186 134 NC_002967:TDE0267|5end_HAM1_protein_299108..299338_NEGATIVE 747 160 111 196 144 NC_002967:TDE1823|5end_hypothetical_protein_1856990..1857220_NEGATIVE 732 160 111 199 148 NC_002967:TDE1796|5end_hypothetical_protein_1835515..1835745_NEGATIVE 711 160 111 229 177 NC_002967:TDE1813|5end_hypothetical_protein_1848249..1848479_NEGATIVE 711 160 111 185 133 NC_002967:TDE1791|5end_hypothetical_protein_1828775..1829005_NEGATIVE 701 160 111 200 148 NC_002967:TDE1832|5end_hypothetical_protein_1865921..1866151_NEGATIVE 698 160 111 175 123 NC_002967:TDE1839|5end_hypothetical_protein_1871221..1871451_NEGATIVE 698 160 111 198 146 NC_002967:TDE1820|5end_hypothetical_protein_1854692..1854922_NEGATIVE 683 160 111 229 177 NC_002967:TDE1790|5end_hypothetical_protein_1828007..1828237_NEGATIVE 677 160 112 168 115 NC_002967:TDE1795|5end_funZ_protein,_putative_1834673..1834903_NEGATIVE 655 160 112 177 122 NC_002967:TDE1803|5end_hypothetical_protein_1839270..1839500_NEGATIVE 640 160 113 175 125 NC_002967:TDE1822|5end_hypothetical_protein_1855922..1856152_NEGATIVE 582 156 111 231 183 NC_002967:TDE1799|5end_hypothetical_protein_1837264..1837494_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 970 160 113 75 28 NC_002967:TDE1839|3end_hypothetical_protein_1870391..1870541_NEGATIVE 750 160 111 89 37 NC_002967:TDE1843|3end_hypothetical_protein_1872199..1872349_NEGATIVE 750 160 111 79 27 NC_002967:TDE1837|3end_hypothetical_protein_1869542..1869692_NEGATIVE 750 160 111 75 23 NC_002967:TDE1832|3end_hypothetical_protein_1865172..1865322_NEGATIVE 750 160 111 80 28 NC_002967:TDE1812|3end_hypothetical_protein_1847580..1847730_NEGATIVE 750 160 111 71 19 NC_002967:TDE1787|3end_hypothetical_protein_1826378..1826528_NEGATIVE 750 160 111 89 37 NC_002967:TDE1780|3end_hypothetical_protein_1822183..1822333_NEGATIVE 750 160 111 75 23 NC_002967:TDE0268|3end_hypothetical_protein_299219..299369_NEGATIVE 746 160 111 106 54 NC_002967:TDE1815|3end_hypothetical_protein_1849458..1849608_NEGATIVE 732 160 111 59 8 NC_002967:TDE1831|3end_hypothetical_protein_1864415..1864565_NEGATIVE 732 160 111 59 8 NC_002967:TDE1797|3end_hypothetical_protein_1835655..1835805_NEGATIVE 731 160 111 84 32 NC_002967:TDE1806|3end_hypothetical_protein_1843190..1843340_NEGATIVE 720 160 111 80 28 NC_002967:TDE1789|3end_hypothetical_protein_1827338..1827488_NEGATIVE 718 160 111 89 37 NC_002967:TDE1793|3end_hydrolase,_carbon-nitrogen_family_1829957..1830107_NEGATIVE 705 160 111 73 21 NC_002967:TDE0097|3end_hypothetical_protein_114801..114951_POSITIVE 701 160 111 79 27 NC_002967:TDE1836|3end_hypothetical_protein_1868514..1868664_NEGATIVE 701 160 111 75 23 NC_002967:TDE1810|3end_hypothetical_protein_1845924..1846074_NEGATIVE 701 160 111 80 28 NC_002967:TDE1808|3end_hypothetical_protein_1844084..1844234_NEGATIVE 698 160 111 72 20 NC_002967:TDE1840|3end_hypothetical_protein_1871324..1871474_NEGATIVE 698 160 111 75 23 NC_002967:TDE1821|3end_hypothetical_protein_1854815..1854965_NEGATIVE