Origin IGS: aaacataaatcctataataaatatattttttttcattttctttcttgttgcccattgggcaatccgtctaacattcgcttaacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttaggcgttttaaactatgataggtctgttaaaattttaagaaacctttatattaataaagag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctctttattaatataaaggtttcttaaaattttaacagacctatcatagtttaaaacgcctaacacccgcttcaacctgacattgcggacaagccgcaaatgcaggttaagcgaatgttagacggattgcccaatgggcaacaagaaagaaaatgaaaaaaaatatatttattataggatttatgttt Mask Tandem Repeat Region ================================================ aaacataaatcctataataaatatattttttttcattttctttcttgttgcccattgggcaatccgtctaacattcgcttaacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttaggcgttttaaactatgataggtctgttaaaattttaagaaacctttatattaataaagag Find is-nt database================================================ Query_seq: TDE1797:TDE1798|TDE1797:TDE1798:hypothetical protein:hypothetical protein:<-<-:1836363..1836550 188 aaacataaatcctataataaatatattttttttcattttctttcttgttgcccattgggcaatccgtctaacattcgcttaacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttaggcgttttaaactatgataggtctgttaaaattttaagaaacctttatattaataaagag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE1797:TDE1798|TDE1797:TDE1798:hypothetical protein:hypothetical protein:<-<-:1836363..1836550 188 aaacataaatcctataataaatatattttttttcattttctttcttgttgcccattgggcaatccgtctaacattcgcttaacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttaggcgttttaaactatgataggtctgttaaaattttaagaaacctttatattaataaagag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE1797:TDE1798|TDE1797:TDE1798:hypothetical protein:hypothetical protein:<-<-:1836363..1836550 188 aaacataaatcctataataaatatattttttttcattttctttcttgttgcccattgggcaatccgtctaacattcgcttaacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttaggcgttttaaactatgataggtctgttaaaattttaagaaacctttatattaataaagag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaacataaatcctataataaatatattttttttcattttctttcttgttgcccattgggcaatccgtctaacattcgcttaacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttaggcgttttaaactatgataggtctgttaaaattttaagaaacctttatattaataaagag Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 25 End: 114 ........................ M F F H F L S C C P L G N P S N I R L T C I C G L S A M S G .......................................................................... Protein_Len: 35 Strand: + Start: 84 End: 188 ................................................................................... M H L R L V R N V R L K R V L G V L N Y D R S V K I L R N L Y I N K E aaacataaatcctataataaatatattttttttcattttctttcttgttgcccattgggcaatccgtctaacattcgcttaacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttaggcgttttaaactatgataggtctgttaaaattttaagaaacctttatattaataaagag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaacataaatcctataataaatatattttttttcattttctttcttgttgcccattgggcaatccgtctaacattcgcttaacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttaggcgttttaaactatgataggtctgttaaaattttaagaaacctttatattaataaagag Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 70 HP_score: -8.9 Tail_Score: -3.09518 Start: 74 End: 123 Full_Region: GCAATCCGTCTAACA TTCGCTTAACCTGCATTTGCGG CTTGT CCGCAATGTCAGGTTGAAGCGGG TGTTAGGCGTTTTAA .........................................................................TTCGCTTAACCTGCATTTGCGGCTTGTCCGCAATGTCAGGTTGAAGCGGG................................................................. Find igs database================================================ Query_seq: TDE1797:TDE1798|TDE1797:TDE1798:hypothetical protein:hypothetical protein:<-<-:1836363..1836550 188 aaacataaatcctataataaatatattttttttcattttctttcttgttgcccattgggcaatccgtctaacattcgcttaacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttaggcgttttaaactatgataggtctgttaaaattttaagaaacctttatattaataaagag Intra-Species Hit: Count: 2 Min: 1 Max: 188 Len: 188 Subject: NC_002967_TDE1797_TDE1798|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1836363,1836550]|188 HSP 1 e-value: 3.0E-88 bit: 325.0 Len: 188 Query Start:1 Query End:188 Subject Strand: POSITIVE Subject Start: 1 Subject End: 188 aaacataaatcctataataaatatannnnnnnncattttctttcttgttgcccattgggcaatccgtctaacattcgcttaacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttaggcgttttaaactatgataggtctgttaaaattttaagaaacctttatattaataaagag aaacataaatcctataataaatatattttttttcattttctttcttgttgcccattgggcaatccgtctaacattcgcttaacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttaggcgttttaaactatgataggtctgttaaaattttaagaaacctttatattaataaagag Subject: NC_002967_TDE1831_TDE1832|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1865123,1865261]|139 HSP 1 e-value: 5.0E-53 bit: 208.0 Len: 129 Query Start:1 Query End:129 Subject Strand: POSITIVE Subject Start: 1 Subject End: 129 aaacataaatcctataataaatatannnnnnnncattttctttcttgttgcccattgggcaatccgtctaacattcgcttaacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttag........................................................... aaacataaatcctataataaatatattttttttcattttctttcttgttgcccattgggcaatccgtctaacattcgcttaacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtgttag........................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAACAUAAAUCCUAUAAUAAAUAUAUUUUUUUUCAUUUUCUUUCUUGUUGCCCAUUGGGCAAUCCGUCUAACAUUCGCUUAACCUGCAUUUGCGGCUUGUCCGCAAUGUCAGGUUGAAGCGGGUGUUAGGCGUUUUAAACUAUGAUAGGUCUGUUAAAAUUUUAAGAAACCUUUAUAUUAAUAAAGAG .((((.....(((((.(((...........................(((((((...))))))).((((((((((((((((((((((((((.((((.....)))))))).))))))).)))))))))))))))........))).)))))..))))...............((((((....)))))).. (-59.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUCUUUAUUAAUAUAAAGGUUUCUUAAAAUUUUAACAGACCUAUCAUAGUUUAAAACGCCUAACACCCGCUUCAACCUGACAUUGCGGACAAGCCGCAAAUGCAGGUUAAGCGAAUGUUAGACGGAUUGCCCAAUGGGCAACAAGAAAGAAAAUGAAAAAAAAUAUAUUUAUUAUAGGAUUUAUGUUU .((((.((.((((((.((((((.((((....)))).)))))).((((..(((....((.((((((..(((((.((((((.((((((((.....)))).)))))))))))))))..)))))).))..((((((...))))))......)))..)))).......)))))).))...))))......... (-53.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1032 130 81 188 139 NC_002967:TDE1827|5end_hydrolase,_carbon-nitrogen_family_1860830..1861060_NEGATIVE 1032 130 81 221 172 NC_002967:TDE1797|5end_hypothetical_protein_1836272..1836502_NEGATIVE 1032 130 81 192 143 NC_002967:TDE0267|5end_HAM1_protein_299108..299338_NEGATIVE 999 129 81 183 135 NC_002967:TDE1836|5end_hypothetical_protein_1869443..1869673_NEGATIVE 999 129 81 220 172 NC_002967:TDE1831|5end_hypothetical_protein_1865032..1865262_NEGATIVE 999 129 81 201 153 NC_002967:TDE1823|5end_hypothetical_protein_1856990..1857220_NEGATIVE 999 129 81 185 137 NC_002967:TDE1817|5end_hypothetical_protein_1852599..1852829_NEGATIVE 999 129 81 182 134 NC_002967:TDE1811|5end_hypothetical_protein_1847483..1847713_NEGATIVE 999 129 81 173 125 NC_002967:TDE1795|5end_funZ_protein,_putative_1834673..1834903_NEGATIVE 990 129 82 180 133 NC_002967:TDE1839|5end_hypothetical_protein_1871221..1871451_NEGATIVE 990 129 82 205 158 NC_002967:TDE1832|5end_hypothetical_protein_1865921..1866151_NEGATIVE 990 129 82 203 156 NC_002967:TDE1820|5end_hypothetical_protein_1854692..1854922_NEGATIVE 943 129 81 204 157 NC_002967:TDE1796|5end_hypothetical_protein_1835515..1835745_NEGATIVE 937 120 71 231 182 NC_002967:TDE1799|5end_hypothetical_protein_1837264..1837494_NEGATIVE 922 129 81 180 132 NC_002967:TDE1822|5end_hypothetical_protein_1855922..1856152_NEGATIVE 918 129 81 190 142 NC_002967:TDE1791|5end_hypothetical_protein_1828775..1829005_NEGATIVE 912 120 71 225 176 NC_002967:TDE1790|5end_hypothetical_protein_1828007..1828237_NEGATIVE 908 120 71 225 176 NC_002967:TDE1813|5end_hypothetical_protein_1848249..1848479_NEGATIVE 859 128 81 181 132 NC_002967:TDE1803|5end_hypothetical_protein_1839270..1839500_NEGATIVE 838 130 81 195 145 NC_002967:TDE0262|5end_hypothetical_protein_296300..296530_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1032 130 81 95 46 NC_002967:TDE1843|3end_hypothetical_protein_1872199..1872349_NEGATIVE 1032 130 81 84 35 NC_002967:TDE1828|3end_hypothetical_protein_1860934..1861084_NEGATIVE 1032 130 81 95 46 NC_002967:TDE1780|3end_hypothetical_protein_1822183..1822333_NEGATIVE 1032 130 81 81 32 NC_002967:TDE0268|3end_hypothetical_protein_299219..299369_NEGATIVE 1023 130 82 81 33 NC_002967:TDE1810|3end_hypothetical_protein_1845924..1846074_NEGATIVE 1004 130 81 84 35 NC_002967:TDE1776|3end_hypothetical_protein_1818414..1818564_NEGATIVE 999 129 81 84 36 NC_002967:TDE1837|3end_hypothetical_protein_1869542..1869692_NEGATIVE 999 129 81 80 32 NC_002967:TDE1832|3end_hypothetical_protein_1865172..1865322_NEGATIVE 999 129 81 85 37 NC_002967:TDE1812|3end_hypothetical_protein_1847580..1847730_NEGATIVE 999 129 81 80 32 NC_002967:TDE1796|3end_hypothetical_protein_1834766..1834916_NEGATIVE 999 129 81 76 28 NC_002967:TDE1787|3end_hypothetical_protein_1826378..1826528_NEGATIVE 990 129 82 77 30 NC_002967:TDE1840|3end_hypothetical_protein_1871324..1871474_NEGATIVE 990 129 82 84 37 NC_002967:TDE1836|3end_hypothetical_protein_1868514..1868664_NEGATIVE 990 129 82 80 33 NC_002967:TDE1821|3end_hypothetical_protein_1854815..1854965_NEGATIVE 990 129 82 85 38 NC_002967:TDE1808|3end_hypothetical_protein_1844084..1844234_NEGATIVE 990 129 82 77 30 NC_002967:TDE1800|3end_hypothetical_protein_1837427..1837577_NEGATIVE 990 130 81 79 30 NC_002967:TDE0097|3end_hypothetical_protein_114801..114951_POSITIVE 989 120 71 76 27 NC_002967:TDE1789|3end_hypothetical_protein_1827338..1827488_NEGATIVE 981 130 81 112 63 NC_002967:TDE1815|3end_hypothetical_protein_1849458..1849608_NEGATIVE 980 129 81 89 41 NC_002967:TDE1806|3end_hypothetical_protein_1843190..1843340_NEGATIVE