Origin IGS: atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttaacctgcatttgcggctcgtccgcaatgtcaggttgaagcgggtg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cacccgcttcaacctgacattgcggacgagccgcaaatgcaggttaagcgaatgttagacttagtttgataatcaccagaaaaagaatatttttttacttacatgattaattaatgtgaatatattacgaatatcatatttatgctaaattttattatgtggctatatttattttgaggtaatgat Mask Tandem Repeat Region ================================================ atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttaacctgcatttgcggctcgtccgcaatgtcaggttgaagcgggtg Find is-nt database================================================ Query_seq: TDE1779:TDE1780|TDE1779:TDE1780:hypothetical protein:hypothetical protein:<-<-:1822087..1822272 186 atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttaacctgcatttgcggctcgtccgcaatgtcaggttgaagcgggtg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE1779:TDE1780|TDE1779:TDE1780:hypothetical protein:hypothetical protein:<-<-:1822087..1822272 186 atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttaacctgcatttgcggctcgtccgcaatgtcaggttgaagcgggtg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE1779:TDE1780|TDE1779:TDE1780:hypothetical protein:hypothetical protein:<-<-:1822087..1822272 186 atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttaacctgcatttgcggctcgtccgcaatgtcaggttgaagcgggtg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttaacctgcatttgcggctcgtccgcaatgtcaggttgaagcgggtg Predict ORF larger than 30AA ================================================ atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttaacctgcatttgcggctcgtccgcaatgtcaggttgaagcgggtg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttaacctgcatttgcggctcgtccgcaatgtcaggttgaagcgggtg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TDE1779:TDE1780|TDE1779:TDE1780:hypothetical protein:hypothetical protein:<-<-:1822087..1822272 186 atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttaacctgcatttgcggctcgtccgcaatgtcaggttgaagcgggtg Intra-Species Hit: Count: 2 Min: 1 Max: 186 Len: 186 Subject: NC_002967_TDE1779_TDE1780|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1822087,1822272]|186 HSP 1 e-value: 8.0E-89 bit: 327.0 Len: 186 Query Start:1 Query End:186 Subject Strand: POSITIVE Subject Start: 1 Subject End: 186 atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtnnnnnnntattctttttctggtgattatcaaactaagtctaacattcgcttaacctgcatttgcggctcgtccgcaatgtcaggttgaagcgggtg atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttaacctgcatttgcggctcgtccgcaatgtcaggttgaagcgggtg Subject: NC_002967_TDE1835_TDE1836|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1868408,1868603]|196 HSP 1 e-value: 4.0E-84 bit: 311.0 Len: 186 Query Start:1 Query End:186 Subject Strand: POSITIVE Subject Start: 1 Subject End: 186 atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtnnnnnnntattctttttctggtgattatcaaactaagtctaacattcgcttaacctgcatttgcggctcgtccgcaatgtcaggttgaagcgggtg atcattacctcaaaataaatatagccacataataaaatttagcataaatatgatattcgtaatatattcacattaattaatcatgtaagtaaaaaaatattctttttctggtgattatcaaactaagtctaacattcgcttgacctgcatttgcggcttgtccgcaatgtcaggttgaagcgggtg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCAUUACCUCAAAAUAAAUAUAGCCACAUAAUAAAAUUUAGCAUAAAUAUGAUAUUCGUAAUAUAUUCACAUUAAUUAAUCAUGUAAGUAAAAAAAUAUUCUUUUUCUGGUGAUUAUCAAACUAAGUCUAACAUUCGCUUAACCUGCAUUUGCGGCUCGUCCGCAAUGUCAGGUUGAAGCGGGUG ...((((((..((((..(((((.((................))....(((((((.....((((........))))....)))))))..........)))))..))))..)))))).................(((((((((((((((((((.((((.....)))))))).))))))).)))))))) (-41.89) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CACCCGCUUCAACCUGACAUUGCGGACGAGCCGCAAAUGCAGGUUAAGCGAAUGUUAGACUUAGUUUGAUAAUCACCAGAAAAAGAAUAUUUUUUUACUUACAUGAUUAAUUAAUGUGAAUAUAUUACGAAUAUCAUAUUUAUGCUAAAUUUUAUUAUGUGGCUAUAUUUAUUUUGAGGUAAUGAU ....(((((.((((((.((((((((.....)))).)))))))))))))))..((((((((...))))))))...(((..((((.((((((........(((((((((...(((..((((((((..((....))..))))))))..))).....)))))))))..)))))).))))..)))...... (-43.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 988 180 131 173 124 NC_002967:TDE1836|5end_hypothetical_protein_1869443..1869673_NEGATIVE 988 180 131 172 123 NC_002967:TDE1811|5end_hypothetical_protein_1847483..1847713_NEGATIVE 988 180 131 181 132 NC_002967:TDE0267|5end_HAM1_protein_299108..299338_NEGATIVE 936 180 131 193 144 NC_002967:TDE1820|5end_hypothetical_protein_1854692..1854922_NEGATIVE 932 180 131 194 146 NC_002967:TDE1796|5end_hypothetical_protein_1835515..1835745_NEGATIVE 912 180 131 210 161 NC_002967:TDE1831|5end_hypothetical_protein_1865032..1865262_NEGATIVE 912 180 131 175 126 NC_002967:TDE1817|5end_hypothetical_protein_1852599..1852829_NEGATIVE 912 180 131 210 161 NC_002967:TDE1797|5end_hypothetical_protein_1836272..1836502_NEGATIVE 911 180 131 224 175 NC_002967:TDE1790|5end_hypothetical_protein_1828007..1828237_NEGATIVE 909 180 131 191 142 NC_002967:TDE1823|5end_hypothetical_protein_1856990..1857220_NEGATIVE 900 180 131 177 128 NC_002967:TDE1827|5end_hydrolase,_carbon-nitrogen_family_1860830..1861060_NEGATIVE 863 180 131 195 146 NC_002967:TDE1832|5end_hypothetical_protein_1865921..1866151_NEGATIVE 860 180 131 170 121 NC_002967:TDE1839|5end_hypothetical_protein_1871221..1871451_NEGATIVE 860 180 131 230 181 NC_002967:TDE1799|5end_hypothetical_protein_1837264..1837494_NEGATIVE 831 180 131 224 175 NC_002967:TDE1813|5end_hypothetical_protein_1848249..1848479_NEGATIVE 831 180 131 180 131 NC_002967:TDE1791|5end_hypothetical_protein_1828775..1829005_NEGATIVE 824 180 131 163 114 NC_002967:TDE1795|5end_funZ_protein,_putative_1834673..1834903_NEGATIVE 776 180 131 170 121 NC_002967:TDE1822|5end_hypothetical_protein_1855922..1856152_NEGATIVE 726 165 127 231 193 NC_002967:TDE1814|5end_hypothetical_protein_1849313..1849543_NEGATIVE 716 180 131 172 121 NC_002967:TDE1803|5end_hypothetical_protein_1839270..1839500_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 988 180 131 74 25 NC_002967:TDE1837|3end_hypothetical_protein_1869542..1869692_NEGATIVE 988 180 131 75 26 NC_002967:TDE1812|3end_hypothetical_protein_1847580..1847730_NEGATIVE 988 180 131 66 17 NC_002967:TDE1787|3end_hypothetical_protein_1826378..1826528_NEGATIVE 988 180 131 70 21 NC_002967:TDE0268|3end_hypothetical_protein_299219..299369_NEGATIVE 969 180 131 79 30 NC_002967:TDE1806|3end_hypothetical_protein_1843190..1843340_NEGATIVE 939 180 131 75 26 NC_002967:TDE1808|3end_hypothetical_protein_1844084..1844234_NEGATIVE 937 180 131 101 52 NC_002967:TDE1815|3end_hypothetical_protein_1849458..1849608_NEGATIVE 936 180 131 70 21 NC_002967:TDE1821|3end_hypothetical_protein_1854815..1854965_NEGATIVE 932 180 131 54 6 NC_002967:TDE1831|3end_hypothetical_protein_1864415..1864565_NEGATIVE 932 180 131 54 6 NC_002967:TDE1797|3end_hypothetical_protein_1835655..1835805_NEGATIVE 912 180 131 84 35 NC_002967:TDE1843|3end_hypothetical_protein_1872199..1872349_NEGATIVE 912 180 131 70 21 NC_002967:TDE1832|3end_hypothetical_protein_1865172..1865322_NEGATIVE 912 180 131 75 26 NC_002967:TDE1789|3end_hypothetical_protein_1827338..1827488_NEGATIVE 900 180 131 73 24 NC_002967:TDE1828|3end_hypothetical_protein_1860934..1861084_NEGATIVE 890 180 131 68 19 NC_002967:TDE0097|3end_hypothetical_protein_114801..114951_POSITIVE 885 180 131 84 35 NC_002967:TDE1793|3end_hydrolase,_carbon-nitrogen_family_1829957..1830107_NEGATIVE 872 180 131 73 24 NC_002967:TDE1776|3end_hypothetical_protein_1818414..1818564_NEGATIVE 863 180 131 74 25 NC_002967:TDE1836|3end_hypothetical_protein_1868514..1868664_NEGATIVE 863 180 131 79 30 NC_002967:TDE1811|3end_hypothetical_protein_1846773..1846923_NEGATIVE 863 180 131 70 21 NC_002967:TDE1810|3end_hypothetical_protein_1845924..1846074_NEGATIVE