Origin IGS: ctcttttaattcaatgtattgatctattctaattttctcaaattttaatttatttctctaaatatttgctaataccattctaagttattttctcaattgctaataaattctcgtgtatattttgagacttctaccttcgtatatttccatgtgccgtaggcatcaacctaactaccgcttaacctacatttgcggctcgtccgcaatgtcaggttgaagcgggtgttaggttgtat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atacaacctaacacccgcttcaacctgacattgcggacgagccgcaaatgtaggttaagcggtagttaggttgatgcctacggcacatggaaatatacgaaggtagaagtctcaaaatatacacgagaatttattagcaattgagaaaataacttagaatggtattagcaaatatttagagaaataaattaaaatttgagaaaattagaatagatcaatacattgaattaaaagag Mask Tandem Repeat Region ================================================ ctcttttaattcaatgtattgatctattctaattttctcaaattttaatttatttctctaaatatttgctaataccattctaagttattttctcaattgctaataaattctcgtgtatattttgagacttctaccttcgtatatttccatgtgccgtaggcatcaacctaactaccgcttaacctacatttgcggctcgtccgcaatgtcaggttgaagcgggtgttaggttgtat Find is-nt database================================================ Query_seq: TDE1775:TDE1776|TDE1775:TDE1776:hypothetical protein:hypothetical protein:<-<-:1818268..1818503 236 ctcttttaattcaatgtattgatctattctaattttctcaaattttaatttatttctctaaatatttgctaataccattctaagttattttctcaattgctaataaattctcgtgtatattttgagacttctaccttcgtatatttccatgtgccgtaggcatcaacctaactaccgcttaacctacatttgcggctcgtccgcaatgtcaggttgaagcgggtgttaggttgtat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE1775:TDE1776|TDE1775:TDE1776:hypothetical protein:hypothetical protein:<-<-:1818268..1818503 236 ctcttttaattcaatgtattgatctattctaattttctcaaattttaatttatttctctaaatatttgctaataccattctaagttattttctcaattgctaataaattctcgtgtatattttgagacttctaccttcgtatatttccatgtgccgtaggcatcaacctaactaccgcttaacctacatttgcggctcgtccgcaatgtcaggttgaagcgggtgttaggttgtat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE1775:TDE1776|TDE1775:TDE1776:hypothetical protein:hypothetical protein:<-<-:1818268..1818503 236 ctcttttaattcaatgtattgatctattctaattttctcaaattttaatttatttctctaaatatttgctaataccattctaagttattttctcaattgctaataaattctcgtgtatattttgagacttctaccttcgtatatttccatgtgccgtaggcatcaacctaactaccgcttaacctacatttgcggctcgtccgcaatgtcaggttgaagcgggtgttaggttgtat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctcttttaattcaatgtattgatctattctaattttctcaaattttaatttatttctctaaatatttgctaataccattctaagttattttctcaattgctaataaattctcgtgtatattttgagacttctaccttcgtatatttccatgtgccgtaggcatcaacctaactaccgcttaacctacatttgcggctcgtccgcaatgtcaggttgaagcgggtgttaggttgtat Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 107 End: 214 .......................................................................................................... M L V Y I L R L L P S Y I S M C R R H Q P N Y R L T Y I C G S S A M S G ...................... ctcttttaattcaatgtattgatctattctaattttctcaaattttaatttatttctctaaatatttgctaataccattctaagttattttctcaattgctaataaattctcgtgtatattttgagacttctaccttcgtatatttccatgtgccgtaggcatcaacctaactaccgcttaacctacatttgcggctcgtccgcaatgtcaggttgaagcgggtgttaggttgtat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctcttttaattcaatgtattgatctattctaattttctcaaattttaatttatttctctaaatatttgctaataccattctaagttattttctcaattgctaataaattctcgtgtatattttgagacttctaccttcgtatatttccatgtgccgtaggcatcaacctaactaccgcttaacctacatttgcggctcgtccgcaatgtcaggttgaagcgggtgttaggttgtat Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 69 HP_score: -7.7 Tail_Score: -3.21199 Start: 172 End: 225 Full_Region: atacaacctaa cacccgcttcaacctgacattgcgg acgag ccgcaaatgtaggttaagcggtag ttaggttgatgccta ...........................................................................................................................................................................cacccgcttcaacctgacattgcggacgagccgcaaatgtaggttaagcggtag........... TransTerm Strand: - Conf: 90 HP_score: -12.4 Tail_Score: -2.79684 Start: 175 End: 222 Full_Region: atacaacctaacac ccgcttcaacctgacattgcgg acgag ccgcaaatgtaggttaagcgg tagttaggttgatgc ..............................................................................................................................................................................ccgcttcaacctgacattgcggacgagccgcaaatgtaggttaagcgg.............. Find igs database================================================ Query_seq: TDE1775:TDE1776|TDE1775:TDE1776:hypothetical protein:hypothetical protein:<-<-:1818268..1818503 236 ctcttttaattcaatgtattgatctattctaattttctcaaattttaatttatttctctaaatatttgctaataccattctaagttattttctcaattgctaataaattctcgtgtatattttgagacttctaccttcgtatatttccatgtgccgtaggcatcaacctaactaccgcttaacctacatttgcggctcgtccgcaatgtcaggttgaagcgggtgttaggttgtat Intra-Species Hit: Count: 1 Min: 1 Max: 236 Len: 236 Subject: NC_002967_TDE1775_TDE1776|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1818268,1818503]|236 HSP 1 e-value: 1.0E-131 bit: 468.0 Len: 236 Query Start:1 Query End:236 Subject Strand: POSITIVE Subject Start: 1 Subject End: 236 ctcttttaattcaatgtattgatctattctaattttctcaaattttaatttatttctctaaatatttgctaataccattctaagttattttctcaattgctaataaattctcgtgtatattttgagacttctaccttcgtatatttccatgtgccgtaggcatcaacctaactaccgcttaacctacatttgcggctcgtccgcaatgtcaggttgaagcgggtgttaggttgtat ctcttttaattcaatgtattgatctattctaattttctcaaattttaatttatttctctaaatatttgctaataccattctaagttattttctcaattgctaataaattctcgtgtatattttgagacttctaccttcgtatatttccatgtgccgtaggcatcaacctaactaccgcttaacctacatttgcggctcgtccgcaatgtcaggttgaagcgggtgttaggttgtat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUCUUUUAAUUCAAUGUAUUGAUCUAUUCUAAUUUUCUCAAAUUUUAAUUUAUUUCUCUAAAUAUUUGCUAAUACCAUUCUAAGUUAUUUUCUCAAUUGCUAAUAAAUUCUCGUGUAUAUUUUGAGACUUCUACCUUCGUAUAUUUCCAUGUGCCGUAGGCAUCAACCUAACUACCGCUUAACCUACAUUUGCGGCUCGUCCGCAAUGUCAGGUUGAAGCGGGUGUUAGGUUGUAU ..........((((....)))).............(((((((.....(((((......)))))........((((.........(((((..(......)..)))))........))))..)))))))......(((..((((((....))))))...)))...(((((((((..((((((((((((((((.((((.....))))))))).)))))).)))))..)))))))))... (-53.33) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUACAACCUAACACCCGCUUCAACCUGACAUUGCGGACGAGCCGCAAAUGUAGGUUAAGCGGUAGUUAGGUUGAUGCCUACGGCACAUGGAAAUAUACGAAGGUAGAAGUCUCAAAAUAUACACGAGAAUUUAUUAGCAAUUGAGAAAAUAACUUAGAAUGGUAUUAGCAAAUAUUUAGAGAAAUAAAUUAAAAUUUGAGAAAAUUAGAAUAGAUCAAUACAUUGAAUUAAAAGAG ...(((((((((..((((((.((((((.((((((((.....)))).))))))))))))))))..))))))))).(((((.((....((....))...)).)))))....(((((((..........(....(((((....(((((.......)))))..)))))....)...(((((....)))))........))))))).........(((.((((....)))).)))...... (-57.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 979 230 181 188 139 NC_002967:TDE1827|5end_hydrolase,_carbon-nitrogen_family_1860830..1861060_NEGATIVE 979 230 181 192 143 NC_002967:TDE0267|5end_HAM1_protein_299108..299338_NEGATIVE 946 229 181 183 135 NC_002967:TDE1836|5end_hypothetical_protein_1869443..1869673_NEGATIVE 946 229 181 182 134 NC_002967:TDE1811|5end_hypothetical_protein_1847483..1847713_NEGATIVE 937 229 182 203 156 NC_002967:TDE1820|5end_hypothetical_protein_1854692..1854922_NEGATIVE 903 230 181 221 172 NC_002967:TDE1797|5end_hypothetical_protein_1836272..1836502_NEGATIVE 890 229 181 204 157 NC_002967:TDE1796|5end_hypothetical_protein_1835515..1835745_NEGATIVE 870 229 181 220 172 NC_002967:TDE1831|5end_hypothetical_protein_1865032..1865262_NEGATIVE 870 229 181 201 153 NC_002967:TDE1823|5end_hypothetical_protein_1856990..1857220_NEGATIVE 870 229 181 185 137 NC_002967:TDE1817|5end_hypothetical_protein_1852599..1852829_NEGATIVE 870 229 181 173 125 NC_002967:TDE1795|5end_funZ_protein,_putative_1834673..1834903_NEGATIVE 862 226 181 231 186 NC_002967:TDE1790|5end_hypothetical_protein_1828007..1828237_NEGATIVE 861 229 182 180 133 NC_002967:TDE1839|5end_hypothetical_protein_1871221..1871451_NEGATIVE 861 229 182 205 158 NC_002967:TDE1832|5end_hypothetical_protein_1865921..1866151_NEGATIVE 793 229 181 180 132 NC_002967:TDE1822|5end_hypothetical_protein_1855922..1856152_NEGATIVE 791 140 91 231 182 NC_002967:TDE1775|5end_hypothetical_protein_1818177..1818407_NEGATIVE 789 229 181 190 142 NC_002967:TDE1791|5end_hypothetical_protein_1828775..1829005_NEGATIVE 776 220 171 173 122 NC_002967:TDE1803|5end_hypothetical_protein_1839270..1839500_NEGATIVE 763 220 175 231 186 NC_002967:TDE1799|5end_hypothetical_protein_1837264..1837494_NEGATIVE 740 226 181 231 186 NC_002967:TDE1813|5end_hypothetical_protein_1848249..1848479_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 979 230 181 84 35 NC_002967:TDE1828|3end_hypothetical_protein_1860934..1861084_NEGATIVE 979 230 181 95 46 NC_002967:TDE1780|3end_hypothetical_protein_1822183..1822333_NEGATIVE 979 230 181 81 32 NC_002967:TDE0268|3end_hypothetical_protein_299219..299369_NEGATIVE 946 229 181 84 36 NC_002967:TDE1837|3end_hypothetical_protein_1869542..1869692_NEGATIVE 946 229 181 85 37 NC_002967:TDE1812|3end_hypothetical_protein_1847580..1847730_NEGATIVE 946 229 181 76 28 NC_002967:TDE1787|3end_hypothetical_protein_1826378..1826528_NEGATIVE 937 229 182 80 33 NC_002967:TDE1821|3end_hypothetical_protein_1854815..1854965_NEGATIVE 937 229 182 85 38 NC_002967:TDE1808|3end_hypothetical_protein_1844084..1844234_NEGATIVE 928 230 181 112 63 NC_002967:TDE1815|3end_hypothetical_protein_1849458..1849608_NEGATIVE 927 229 181 89 41 NC_002967:TDE1806|3end_hypothetical_protein_1843190..1843340_NEGATIVE 924 229 181 94 46 NC_002967:TDE1793|3end_hydrolase,_carbon-nitrogen_family_1829957..1830107_NEGATIVE 918 229 181 89 41 NC_002967:TDE1811|3end_hypothetical_protein_1846773..1846923_NEGATIVE 903 230 181 95 46 NC_002967:TDE1843|3end_hypothetical_protein_1872199..1872349_NEGATIVE 894 230 182 81 33 NC_002967:TDE1810|3end_hypothetical_protein_1845924..1846074_NEGATIVE 890 229 181 64 17 NC_002967:TDE1831|3end_hypothetical_protein_1864415..1864565_NEGATIVE 890 229 181 64 17 NC_002967:TDE1797|3end_hypothetical_protein_1835655..1835805_NEGATIVE 870 229 181 80 32 NC_002967:TDE1832|3end_hypothetical_protein_1865172..1865322_NEGATIVE 870 229 181 80 32 NC_002967:TDE1796|3end_hypothetical_protein_1834766..1834916_NEGATIVE 861 229 182 77 30 NC_002967:TDE1840|3end_hypothetical_protein_1871324..1871474_NEGATIVE 861 229 182 84 37 NC_002967:TDE1836|3end_hypothetical_protein_1868514..1868664_NEGATIVE