Origin IGS: ataatcaataacttaatcaataactttgtcaacttacccccattgaccggttaaaattatattaaaatacaaattcagaattttttaagaaaattaaatttatttttgttaaaagtactattgtttttaactaaaagctataatataatggaggcaggaggaaaactaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttagttttcctcctgcctccattatattatagcttttagttaaaaacaatagtacttttaacaaaaataaatttaattttcttaaaaaattctgaatttgtattttaatataattttaaccggtcaatgggggtaagttgacaaagttattgattaagttattgattat Mask Tandem Repeat Region ================================================ annnnnnnnnnnnnnnnnnnnnnnnntgtcaacttacccccattgaccggttaaaattatattaaaatacaaattcagaattttttaagaaaattaaatttatttttgttaaaagtactattgtttttaactaaaagctataatataatggaggcaggaggaaaactaa Find is-nt database================================================ Query_seq: TDE1699:TDE1700|TDE1699:TDE1700:hypothetical protein:hypothetical protein:<-->:1758011..1758179 169 ataatcaataacttaatcaataactttgtcaacttacccccattgaccggttaaaattatattaaaatacaaattcagaattttttaagaaaattaaatttatttttgttaaaagtactattgtttttaactaaaagctataatataatggaggcaggaggaaaactaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE1699:TDE1700|TDE1699:TDE1700:hypothetical protein:hypothetical protein:<-->:1758011..1758179 169 ataatcaataacttaatcaataactttgtcaacttacccccattgaccggttaaaattatattaaaatacaaattcagaattttttaagaaaattaaatttatttttgttaaaagtactattgtttttaactaaaagctataatataatggaggcaggaggaaaactaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE1699:TDE1700|TDE1699:TDE1700:hypothetical protein:hypothetical protein:<-->:1758011..1758179 169 ataatcaataacttaatcaataactttgtcaacttacccccattgaccggttaaaattatattaaaatacaaattcagaattttttaagaaaattaaatttatttttgttaaaagtactattgtttttaactaaaagctataatataatggaggcaggaggaaaactaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ataatcaataacttaatcaataactttgtcaacttacccccattgaccggttaaaattatattaaaatacaaattcagaattttttaagaaaattaaatttatttttgttaaaagtactattgtttttaactaaaagctataatataatggaggcaggaggaaaactaa Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 61 End: 168 ............................................................ M K I Q I Q N F L R K L N L F L L K V L L F L T K S Y N I M E A G G K L . ataatcaataacttaatcaataactttgtcaacttacccccattgaccggttaaaattatattaaaatacaaattcagaattttttaagaaaattaaatttatttttgttaaaagtactattgtttttaactaaaagctataatataatggaggcaggaggaaaactaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 82 Start: 120 End: 149 .......................................................................................................................ATTGTTTTTAACTAAAAGCTATAATATAAT.................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ataatcaataacttaatcaataactttgtcaacttacccccattgaccggttaaaattatattaaaatacaaattcagaattttttaagaaaattaaatttatttttgttaaaagtactattgtttttaactaaaagctataatataatggaggcaggaggaaaactaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TDE1699:TDE1700|TDE1699:TDE1700:hypothetical protein:hypothetical protein:<-->:1758011..1758179 169 ataatcaataacttaatcaataactttgtcaacttacccccattgaccggttaaaattatattaaaatacaaattcagaattttttaagaaaattaaatttatttttgttaaaagtactattgtttttaactaaaagctataatataatggaggcaggaggaaaactaa Intra-Species Hit: Count: 1 Min: 1 Max: 169 Len: 169 Subject: NC_002967_TDE1699_TDE1700|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[1758011,1758179]|169 HSP 1 e-value: 3.0E-20 bit: 99.6 Len: 50 Query Start:1 Query End:50 Subject Strand: POSITIVE Subject Start: 1 Subject End: 50 ataatcaataacttaatcaataactttgtcaacttacccccattgaccgg....................................................................................................................... ataatcaataacttaatcaataactttgtcaacttacccccattgaccgg....................................................................................................................... HSP 2 e-value: 4.0E-10 bit: 65.9 Len: 33 Query Start:137 Query End:169 Subject Strand: POSITIVE Subject Start: 137 Subject End: 169 ........................................................................................................................................gctataatataatggaggcaggaggaaaactaa ........................................................................................................................................gctataatataatggaggcaggaggaaaactaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAUCAAUAACUUAAUCAAUAACUUUGUCAACUUACCCCCAUUGACCGGUUAAAAUUAUAUUAAAAUACAAAUUCAGAAUUUUUUAAGAAAAUUAAAUUUAUUUUUGUUAAAAGUACUAUUGUUUUUAACUAAAAGCUAUAAUAUAAUGGAGGCAGGAGGAAAACUAA ...........(((..((..(((((..(((((..........))))).)))))..(((((((((............((.((((((...((((((.......))))))...)))))).))...((((((....))))))..))))))))).)).....)))......... (-19.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAGUUUUCCUCCUGCCUCCAUUAUAUUAUAGCUUUUAGUUAAAAACAAUAGUACUUUUAACAAAAAUAAAUUUAAUUUUCUUAAAAAAUUCUGAAUUUGUAUUUUAAUAUAAUUUUAACCGGUCAAUGGGGGUAAGUUGACAAAGUUAUUGAUUAAGUUAUUGAUUAU ...(((...((..((((((((((...............(((((((((....))..)))))))....((((((((((((((.....)))))..)))))))))........................))))))))))))..)))........(((((((....))))))). (-24.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 800 50 1 141 92 NC_002967:TDE1699|5end_hypothetical_protein_1757920..1758150_NEGATIVE 270 52 24 120 83 NC_002967:TDE0785|5end_ribosomal_protein_L30_814417..814647_POSITIVE 251 57 22 148 117 NC_002967:TDE2047|5end_hypothetical_protein_2076351..2076581_NEGATIVE 240 52 13 174 120 NC_002967:TDE2714|5end_hypothetical_protein_2772618..2772848_NEGATIVE 220 52 17 173 135 NC_002967:TDE1455|5end_prevent-host-death_family_protein_1502865..1503095_POSITIVE 218 160 114 194 150 NC_002967:TDE0719|5end_bacteriocin_ABC_transporter,_ATP-binding/permease_protein,_putative_753462..753692_POSITIVE 213 160 116 54 7 NC_002967:TDE0683|5end_hypothetical_protein_721303..721533_NEGATIVE 212 78 40 196 155 NC_002967:TDE0176|5end_hypothetical_protein_209798..210028_NEGATIVE 211 159 122 220 187 NC_002967:TDE2465|5end_hypothetical_protein_2489651..2489881_NEGATIVE 211 55 23 183 149 NC_002967:TDE1651|5end_BioY_family_protein_1698732..1698962_NEGATIVE 207 55 24 201 170 NC_002967:TDE1678|5end_ribosomal_protein_S6_1730483..1730713_NEGATIVE 202 62 29 227 194 NC_002967:TDE2520|5end_hypothetical_protein_2547798..2548028_NEGATIVE 199 160 120 69 21 NC_002967:TDE1434|5end_hypothetical_protein_1478462..1478692_NEGATIVE 198 54 12 171 128 NC_002967:TDE0865|5end_hypothetical_protein_887127..887357_POSITIVE 193 51 19 104 64 NC_002967:TDE2740|5end_type_I_restriction-modification_system,_S_subunit,_truncation_2795202..2795432_POSITIVE 193 49 22 53 19 NC_002967:TDE2526|5end_hypothetical_protein_2555034..2555264_NEGATIVE 193 160 116 180 136 NC_002967:TDE1992|5end_OmpA_family_protein_2009464..2009694_POSITIVE 193 89 41 174 129 NC_002967:TDE0034|5end_hypothetical_protein_41450..41680_NEGATIVE 192 63 31 76 40 NC_002967:TDE1441|5end_dTDP-glucose_4,6-dehydratase_1486247..1486477_POSITIVE 189 51 38 137 124 NC_002967:TDE0016|5end_ATPase,_AAA_family_20457..20687_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 270 52 24 115 78 NC_002967:TDE0784|3end_ribosomal_protein_S5_814492..814642_POSITIVE 220 52 17 110 72 NC_002967:TDE1454|3end_sugar_transporter_1502882..1503032_POSITIVE 218 160 114 150 106 NC_002967:TDE0718|3end_hypothetical_protein_753498..753648_POSITIVE 211 55 23 58 24 NC_002967:TDE1652|3end_ABC_transporter,_ATP-binding/permease_protein_1698857..1699007_NEGATIVE 207 55 24 57 26 NC_002967:TDE1679|3end_ATP_synthase_subunit_K_1730627..1730777_NEGATIVE 202 62 29 70 37 NC_002967:TDE2521|3end_hypothetical_protein_2547955..2548105_NEGATIVE 198 56 11 149 93 NC_002967:TDE2434|3end_polysaccharide_deacetylase_family_protein_2463300..2463450_NEGATIVE 198 51 12 138 97 NC_002967:TDE1862|3end_hypothetical_protein_1887979..1888129_POSITIVE 198 54 12 141 98 NC_002967:TDE0864|3end_phosphohexose_mutase_family_protein_887177..887327_POSITIVE 192 63 31 57 21 NC_002967:TDE1440|3end_glucose-1-phosphate_thymidylyltransferase_1486308..1486458_POSITIVE 189 51 38 144 131 NC_002967:TDE0015|3end_lipoprotein,_putative_20544..20694_POSITIVE 183 89 43 44 7 NC_002967:TDE0035|3end_acetyltransferase,_GNAT_family_41580..41730_NEGATIVE 182 62 23 83 38 NC_002967:TDE0559|3end_hypothetical_protein_594126..594276_POSITIVE 177 52 12 90 58 NC_002967:TDE2639|3end_oligoendopeptidase_F_2680089..2680239_POSITIVE 177 52 23 119 75 NC_002967:TDE2246|3end_hypothetical_protein_2286454..2286604_POSITIVE 175 65 24 49 2 NC_002967:TDE2750|3end_GTP-binding_protein_HflX,_truncation_2807288..2807438_POSITIVE 174 49 12 111 83 NC_002967:TDE1598|3end_ABC_transporter,_ATP-binding_protein_1647402..1647552_NEGATIVE 173 61 32 124 93 NC_002967:TDE1949|3end_ABC_transporter,_ATP-binding_protein_1964544..1964694_NEGATIVE 173 159 133 65 34 NC_002967:TDE0044|3end_ABC_transporter,_ATP-binding_protein_51660..51810_NEGATIVE 172 50 26 137 112 NC_002967:TDE2545|3end_hypothetical_protein_2571170..2571320_NEGATIVE