Origin IGS: gaggaaaataaactgacaaagatacagcttcgggctttttacggatttttaatttcagcggaatctcattccaaagcaacagctcaaacctatattaatact .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agtattaatataggtttgagctgttgctttggaatgagattccgctgaaattaaaaatccgtaaaaagcccgaagctgtatctttgtcagtttattttcctc Mask Tandem Repeat Region ================================================ gaggaaaataaactgacaaagatacagcttcgggctttttacggatttttaatttcagcggaatctcattccaaagcaacagctcaaacctatattaatact Find is-nt database================================================ Query_seq: TDE1204:TDE1205|TDE1204:TDE1205:cell division protein FtsZ:integrase/recombinase XerD:->->:1237727..1237828 102 gaggaaaataaactgacaaagatacagcttcgggctttttacggatttttaatttcagcggaatctcattccaaagcaacagctcaaacctatattaatact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE1204:TDE1205|TDE1204:TDE1205:cell division protein FtsZ:integrase/recombinase XerD:->->:1237727..1237828 102 gaggaaaataaactgacaaagatacagcttcgggctttttacggatttttaatttcagcggaatctcattccaaagcaacagctcaaacctatattaatact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE1204:TDE1205|TDE1204:TDE1205:cell division protein FtsZ:integrase/recombinase XerD:->->:1237727..1237828 102 gaggaaaataaactgacaaagatacagcttcgggctttttacggatttttaatttcagcggaatctcattccaaagcaacagctcaaacctatattaatact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gaggaaaataaactgacaaagatacagcttcgggctttttacggatttttaatttcagcggaatctcattccaaagcaacagctcaaacctatattaatact Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 13 End: 102 ............ M T K I Q L R A F Y G F L I S A E S H S K A T A Q T Y I N T gaggaaaataaactgacaaagatacagcttcgggctttttacggatttttaatttcagcggaatctcattccaaagcaacagctcaaacctatattaatact Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gaggaaaataaactgacaaagatacagcttcgggctttttacggatttttaatttcagcggaatctcattccaaagcaacagctcaaacctatattaatact Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TDE1204:TDE1205|TDE1204:TDE1205:cell division protein FtsZ:integrase/recombinase XerD:->->:1237727..1237828 102 gaggaaaataaactgacaaagatacagcttcgggctttttacggatttttaatttcagcggaatctcattccaaagcaacagctcaaacctatattaatact Intra-Species Hit: Count: 1 Min: 1 Max: 102 Len: 102 Subject: NC_002967_TDE1204_TDE1205|cell division protein FtsZ:integrase/recombinase XerD|POSITIVE:POSITIVE|[1237727,1237828]|102 HSP 1 e-value: 2.0E-51 bit: 202.0 Len: 102 Query Start:1 Query End:102 Subject Strand: POSITIVE Subject Start: 1 Subject End: 102 gaggaaaataaactgacaaagatacagcttcgggctttttacggatttttaatttcagcggaatctcattccaaagcaacagctcaaacctatattaatact gaggaaaataaactgacaaagatacagcttcgggctttttacggatttttaatttcagcggaatctcattccaaagcaacagctcaaacctatattaatact Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAGGAAAAUAAACUGACAAAGAUACAGCUUCGGGCUUUUUACGGAUUUUUAAUUUCAGCGGAAUCUCAUUCCAAAGCAACAGCUCAAACCUAUAUUAAUACU .(((........(((.........)))....(((((......(((........)))...(((((...)))))........)))))...)))........... (-13.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUAUUAAUAUAGGUUUGAGCUGUUGCUUUGGAAUGAGAUUCCGCUGAAAUUAAAAAUCCGUAAAAAGCCCGAAGCUGUAUCUUUGUCAGUUUAUUUUCCUC ...........(((..(((((((..(((((...(((.((((..............)))))))..))))).(((((......))))).)))))))....))). (-15.44) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 294 85 43 136 92 NC_002967:TDE2037|5end_ATP:guanido_phosphotransferase_domain_protein_2067720..2067950_NEGATIVE 294 70 26 92 47 NC_002967:TDE0884|5end_tRNA_(guanine-N(1)-)-methyltransferase_902221..902451_POSITIVE 283 74 31 147 110 NC_002967:TDE2732|5end_hypothetical_protein_2788448..2788678_POSITIVE 280 94 56 80 49 NC_002967:TDE0690|5end_tRNA_(5-methylaminomethyl-2-thiouridylate)-methyltransferase_726898..727128_POSITIVE 275 69 25 215 165 NC_002967:TDE2595|5end_hypothetical_protein_2631080..2631310_NEGATIVE 267 64 32 209 179 NC_002967:TDE0606|5end_ParB_family_protein_644132..644362_NEGATIVE 245 60 18 55 6 NC_002967:TDE2736|5end_hypothetical_protein_2791812..2792042_NEGATIVE 241 46 3 192 153 NC_002967:TDE2094|5end_hypothetical_protein_2121319..2121549_NEGATIVE 237 70 26 164 97 NC_002967:TDE2506|5end_radical_SAM_domain_protein_2530581..2530811_POSITIVE 232 70 27 191 142 NC_002967:TDE1918|5end_hypothetical_protein_1938344..1938574_NEGATIVE 232 61 24 183 132 NC_002967:TDE0535|5end_ABC_transporter,_ATP-binding_protein_577323..577553_POSITIVE 231 57 17 229 195 NC_002967:TDE1576|5end_tRNA-i(6)A37_modification_enzyme_MiaB_1622778..1623008_POSITIVE 230 77 31 211 165 NC_002967:TDE1585|5end_hypothetical_protein_1633399..1633629_NEGATIVE 230 66 27 167 128 NC_002967:TDE0066|5end_hypothetical_protein_73926..74156_NEGATIVE 229 69 25 217 164 NC_002967:TDE1878|5end_hydrolase,_haloacid_dehalogenase-like_family_1900745..1900975_NEGATIVE 227 86 42 90 46 NC_002967:TDE2461|5end_selenide,_water_dikinase_(selenocysteine-containing)_2488260..2488490_NEGATIVE 227 65 27 147 110 NC_002967:TDE0135|5end_pyridine_nucleotide-disulphide_oxidoreductase_family_protein_154927..155157_POSITIVE 226 69 27 164 107 NC_002967:TDE0774|5end_50S_ribosomal_protein_L16_810165..810395_POSITIVE 224 70 26 150 118 NC_002967:TDE1198|5end_S-adenosyl-methyltransferase_MraW_1230392..1230622_POSITIVE 224 78 31 142 94 NC_002967:TDE0191|5end_acetyltransferase,_GNAT_family_227806..228036_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 294 70 26 95 50 NC_002967:TDE0883|3end_16S_rRNA_processing_protein_RimM_902304..902454_POSITIVE 283 74 31 72 35 NC_002967:TDE2731|3end_TIM-barrel_protein,_putative,_NifR3_family_2788453..2788603_POSITIVE 280 94 56 74 43 NC_002967:TDE0689|3end_5-methylthioribose_kinase_726972..727122_POSITIVE 276 77 31 130 71 NC_002967:TDE0860|3end_ribosomal_small_subunit_pseudouridine_synthase_A_881840..881990_POSITIVE 268 84 43 151 108 NC_002967:TDE2038|3end_UVR_domain_protein_2067704..2067854_NEGATIVE 259 68 27 142 110 NC_002967:TDE1906|3end_hypothetical_protein_1924727..1924877_POSITIVE 251 69 30 129 81 NC_002967:TDE0026|3end_TraB_family_protein_31826..31976_NEGATIVE 237 70 26 124 57 NC_002967:TDE2505|3end_hypothetical_protein_2530621..2530771_POSITIVE 227 86 42 45 1 NC_002967:TDE2462|3end_hypothetical_protein_2488305..2488455_NEGATIVE 227 68 25 81 11 NC_002967:TDE1953|3end_transcriptional_regulator,_TetR_family_1969030..1969180_NEGATIVE 227 65 27 150 113 NC_002967:TDE0134|3end_oxidoreductase,_FAD-dependent_155010..155160_POSITIVE 226 68 31 139 94 NC_002967:TDE2243|3end_CutC_family_protein_2282214..2282364_NEGATIVE 226 61 27 122 78 NC_002967:TDE0353|3end_lipase/acylhydrolase,_GDSL_family_396743..396893_NEGATIVE 224 70 26 144 112 NC_002967:TDE1197|3end_conserved_hypothetical_protein_TIGR00242_1230466..1230616_POSITIVE 224 78 31 131 83 NC_002967:TDE0192|3end_hypothetical_protein_227817..227967_NEGATIVE 222 84 57 65 36 NC_002967:TDE1694|3end_hypothetical_protein_1749223..1749373_NEGATIVE 221 65 28 141 105 NC_002967:TDE0317|3end_hypothetical_protein_351116..351266_POSITIVE 219 67 23 102 62 NC_002967:TDE1460|3end_hypothetical_protein_1508707..1508857_NEGATIVE 219 74 31 136 99 NC_002967:TDE1035|3end_transcriptional_regulator,_PadR_family_1063860..1064010_POSITIVE 219 59 26 147 118 NC_002967:TDE0838|3end_Na(+)-translocating_NADH-quinone_reductase,_A_subunit_857109..857259_NEGATIVE