Origin IGS: ataaaaatggaaaaagaagtcgaaagttttaaaggaacaaaaacggccgaaatgattttgcttttggacaaggtctataaagaaattgaagaagccgagaaggagtgg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ccactccttctcggcttcttcaatttctttatagaccttgtccaaaagcaaaatcatttcggccgtttttgttcctttaaaactttcgacttctttttccatttttat Mask Tandem Repeat Region ================================================ ataaaaatggaaaaagaagtcgaaagttttaaaggaacaaaaacggccgaaatgattttgcttttggacaaggtctataaagaaattgaagaagccgagaaggagtgg Find is-nt database================================================ Query_seq: TDE0846:TDE0847|TDE0846:TDE0847:hypothetical protein:hypothetical protein:->->:870926..871033 108 ataaaaatggaaaaagaagtcgaaagttttaaaggaacaaaaacggccgaaatgattttgcttttggacaaggtctataaagaaattgaagaagccgagaaggagtgg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE0846:TDE0847|TDE0846:TDE0847:hypothetical protein:hypothetical protein:->->:870926..871033 108 ataaaaatggaaaaagaagtcgaaagttttaaaggaacaaaaacggccgaaatgattttgcttttggacaaggtctataaagaaattgaagaagccgagaaggagtgg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE0846:TDE0847|TDE0846:TDE0847:hypothetical protein:hypothetical protein:->->:870926..871033 108 ataaaaatggaaaaagaagtcgaaagttttaaaggaacaaaaacggccgaaatgattttgcttttggacaaggtctataaagaaattgaagaagccgagaaggagtgg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ataaaaatggaaaaagaagtcgaaagttttaaaggaacaaaaacggccgaaatgattttgcttttggacaaggtctataaagaaattgaagaagccgagaaggagtgg Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 1 End: 108 M K M E K E V E S F K G T K T A E M I L L L D K V Y K E I E E A E K E W ataaaaatggaaaaagaagtcgaaagttttaaaggaacaaaaacggccgaaatgattttgcttttggacaaggtctataaagaaattgaagaagccgagaaggagtgg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ataaaaatggaaaaagaagtcgaaagttttaaaggaacaaaaacggccgaaatgattttgcttttggacaaggtctataaagaaattgaagaagccgagaaggagtgg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TDE0846:TDE0847|TDE0846:TDE0847:hypothetical protein:hypothetical protein:->->:870926..871033 108 ataaaaatggaaaaagaagtcgaaagttttaaaggaacaaaaacggccgaaatgattttgcttttggacaaggtctataaagaaattgaagaagccgagaaggagtgg Intra-Species Hit: Count: 1 Min: 1 Max: 108 Len: 108 Subject: NC_002967_TDE0846_TDE0847|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[870926,871033]|108 HSP 1 e-value: 4.0E-55 bit: 214.0 Len: 108 Query Start:1 Query End:108 Subject Strand: POSITIVE Subject Start: 1 Subject End: 108 ataaaaatggaaaaagaagtcgaaagttttaaaggaacaaaaacggccgaaatgattttgcttttggacaaggtctataaagaaattgaagaagccgagaaggagtgg ataaaaatggaaaaagaagtcgaaagttttaaaggaacaaaaacggccgaaatgattttgcttttggacaaggtctataaagaaattgaagaagccgagaaggagtgg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAAAAUGGAAAAAGAAGUCGAAAGUUUUAAAGGAACAAAAACGGCCGAAAUGAUUUUGCUUUUGGACAAGGUCUAUAAAGAAAUUGAAGAAGCCGAGAAGGAGUGG .................(.((....((((.....)))).....((((......((((((.....(((((...)))))....))))))......)))).....)).).. (-14.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CCACUCCUUCUCGGCUUCUUCAAUUUCUUUAUAGACCUUGUCCAAAAGCAAAAUCAUUUCGGCCGUUUUUGUUCCUUUAAAACUUUCGACUUCUUUUUCCAUUUUUAU .............((((...(((..(((....)))..))).....)))).........((((..(((((.........)))))..))))................... ( -9.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 282 73 33 168 127 NC_002967:TDE2660|5end_hypothetical_protein_2697517..2697747_NEGATIVE 272 92 54 66 24 NC_002967:TDE0066|5end_hypothetical_protein_73926..74156_NEGATIVE 268 96 60 85 51 NC_002967:TDE1474|5end_hypothetical_protein_1521353..1521583_NEGATIVE 260 100 61 206 175 NC_002967:TDE1864|5end_crossover_junction_endodeoxyribonuclease_RuvC_1889108..1889338_NEGATIVE 259 79 43 184 153 NC_002967:TDE0169|5end_methyl-accepting_chemotaxis_domain_protein_198792..199022_NEGATIVE 258 100 52 207 154 NC_002967:TDE0145|5end_thiamine_ABC_transporter,_ATP-binding_protein_166700..166930_POSITIVE 257 83 41 229 179 NC_002967:TDE1383|5end_hypothetical_protein_1422301..1422531_POSITIVE 240 51 24 223 199 NC_002967:TDE1742|5end_AMP-binding_enzyme_family_protein_1797578..1797808_NEGATIVE 237 100 68 146 113 NC_002967:TDE2426|5end_transcription_antitermination_protein_NusG_2455799..2456029_NEGATIVE 237 87 44 193 153 NC_002967:TDE1644|5end_methionyl-tRNA_formyltransferase_1695546..1695776_NEGATIVE 230 78 31 177 124 NC_002967:TDE2655|5end_hypothetical_protein_2693070..2693300_NEGATIVE 230 82 44 65 10 NC_002967:TDE1000|5end_3-hydroxyacid_dehydrogenase_family_protein_1026763..1026993_POSITIVE 228 93 54 56 22 NC_002967:TDE1920|5end_lipoprotein,_putative_1940277..1940507_NEGATIVE 227 74 34 195 151 NC_002967:TDE0858|5end_hypothetical_protein_879518..879748_POSITIVE 225 100 51 187 135 NC_002967:TDE0704|5end_SPFH_domain/Band_7_family_protein_739346..739576_NEGATIVE 223 99 61 201 154 NC_002967:TDE1721|5end_radical_SAM_domain_protein_1775140..1775370_POSITIVE 223 88 46 226 182 NC_002967:TDE0334|5end_hypothetical_protein_374048..374278_NEGATIVE 222 67 21 69 16 NC_002967:TDE1984|5end_hypothetical_protein_1997565..1997795_NEGATIVE 222 61 21 85 46 NC_002967:TDE1132|5end_chorismate_synthase_1163415..1163645_POSITIVE 220 82 46 180 145 NC_002967:TDE1946|5end_hypothetical_protein_1962494..1962724_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 331 90 42 150 93 NC_002967:TDE0992|3end_5-formyltetrahydrofolate_cyclo-ligase_family_protein_1018321..1018471_NEGATIVE 272 92 54 51 9 NC_002967:TDE0067|3end_cyclic_nucleotide-binding_protein_73941..74091_NEGATIVE 268 96 60 104 70 NC_002967:TDE1475|3end_flagellar_filament_core_protein_1521334..1521484_NEGATIVE 250 76 31 71 20 NC_002967:TDE0009|3end_hypothetical_protein_13724..13874_NEGATIVE 243 98 52 67 25 NC_002967:TDE0189|3end_hypothetical_protein_225672..225822_NEGATIVE 237 100 68 142 109 NC_002967:TDE2427|3end_preprotein_translocase,_SecE_subunit_2455803..2455953_NEGATIVE 235 80 32 140 85 NC_002967:TDE2461|3end_selenide,_water_dikinase_(selenocysteine-containing)_2487196..2487346_NEGATIVE 227 93 54 56 14 NC_002967:TDE1907|3end_holo-(acyl-carrier-protein)_synthase_1924726..1924876_NEGATIVE 225 63 25 79 40 NC_002967:TDE2570|3end_ribonuclease_HI_2597937..2598087_POSITIVE 222 67 21 79 26 NC_002967:TDE1985|3end_hypothetical_protein_1997555..1997705_NEGATIVE 222 61 21 121 82 NC_002967:TDE1131|3end_hypothetical_protein_1163531..1163681_POSITIVE 220 49 8 72 40 NC_002967:TDE1918|3end_hypothetical_protein_1937391..1937541_NEGATIVE 217 94 52 49 12 NC_002967:TDE1785|3end_hypothetical_protein_1825194..1825344_POSITIVE 216 84 46 150 110 NC_002967:TDE0335|3end_ribonucleotide-diphosphate_reductase_beta_subunit_374120..374270_NEGATIVE 215 96 60 67 25 NC_002967:TDE1350|3end_rod_shape-determining_protein_MreC_1391270..1391420_POSITIVE 214 50 7 104 59 NC_002967:TDE0950|3end_hypothetical_protein_974166..974316_NEGATIVE 211 100 70 47 14 NC_002967:TDE0991|3end_ribosomal_protein_L28_1017874..1018024_NEGATIVE 211 68 31 58 22 NC_002967:TDE0757|3end_iron_compound_ABC_transporter,_permease_protein,_putative_796859..797009_NEGATIVE 210 70 44 151 127 NC_002967:TDE0985|3end_oligopeptide/dipeptide_ABC_transporter,_periplasmic_peptide-binding_protein,_putative_1013054..1013204_POSITIVE 209 82 44 89 48 NC_002967:TDE0824|3end_lipoprotein,_putative_845226..845376_NEGATIVE