Origin IGS: cggaaaaataccaatcttgattaaatcctttaaagtcgatacgttttcttgtagtccttgtttaatcttggtattttgtgtaaaattatgtgggtacaaatattgattgccatattaagatattatttttatggaagataaggagaaatat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atatttctccttatcttccataaaaataatatcttaatatggcaatcaatatttgtacccacataattttacacaaaataccaagattaaacaaggactacaagaaaacgtatcgactttaaaggatttaatcaagattggtatttttccg Mask Tandem Repeat Region ================================================ cggaaaaataccaatcttgattaaatcctttaaagtcgatacgttttcttgtagtccttgtttaatcttggtattttgtgtaaaattatgtgggtacaaatattgattgccatattaagatattatttttatggaagataaggagaaatat Find is-nt database================================================ Query_seq: TDE0822:TDE0823|TDE0822:TDE0823:hypothetical protein:(3R)-hydroxymyristoyl-(acyl-carrier-protein) dehydratase, putative:->->:844730..844880 151 cggaaaaataccaatcttgattaaatcctttaaagtcgatacgttttcttgtagtccttgtttaatcttggtattttgtgtaaaattatgtgggtacaaatattgattgccatattaagatattatttttatggaagataaggagaaatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE0822:TDE0823|TDE0822:TDE0823:hypothetical protein:(3R)-hydroxymyristoyl-(acyl-carrier-protein) dehydratase, putative:->->:844730..844880 151 cggaaaaataccaatcttgattaaatcctttaaagtcgatacgttttcttgtagtccttgtttaatcttggtattttgtgtaaaattatgtgggtacaaatattgattgccatattaagatattatttttatggaagataaggagaaatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE0822:TDE0823|TDE0822:TDE0823:hypothetical protein:(3R)-hydroxymyristoyl-(acyl-carrier-protein) dehydratase, putative:->->:844730..844880 151 cggaaaaataccaatcttgattaaatcctttaaagtcgatacgttttcttgtagtccttgtttaatcttggtattttgtgtaaaattatgtgggtacaaatattgattgccatattaagatattatttttatggaagataaggagaaatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cggaaaaataccaatcttgattaaatcctttaaagtcgatacgttttcttgtagtccttgtttaatcttggtattttgtgtaaaattatgtgggtacaaatattgattgccatattaagatattatttttatggaagataaggagaaatat Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 8 End: 103 ....... M P I L I K S F K V D T F S C S P C L I L V F C V K L C G Y K Y ................................................ Protein_Len: 33 Strand: - Start: 1 End: 99 P F I G I K I L D K L T S V N E Q L G Q K I K T N Q T F N H P Y M .................................................... cggaaaaataccaatcttgattaaatcctttaaagtcgatacgttttcttgtagtccttgtttaatcttggtattttgtgtaaaattatgtgggtacaaatattgattgccatattaagatattatttttatggaagataaggagaaatat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 57 End: 86 ........................................................CTTGTTTAATCTTGGTATTTTGTGTAAAAT................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cggaaaaataccaatcttgattaaatcctttaaagtcgatacgttttcttgtagtccttgtttaatcttggtattttgtgtaaaattatgtgggtacaaatattgattgccatattaagatattatttttatggaagataaggagaaatat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TDE0822:TDE0823|TDE0822:TDE0823:hypothetical protein:(3R)-hydroxymyristoyl-(acyl-carrier-protein) dehydratase, putative:->->:844730..844880 151 cggaaaaataccaatcttgattaaatcctttaaagtcgatacgttttcttgtagtccttgtttaatcttggtattttgtgtaaaattatgtgggtacaaatattgattgccatattaagatattatttttatggaagataaggagaaatat Intra-Species Hit: Count: 1 Min: 1 Max: 151 Len: 151 Subject: NC_002967_TDE0822_TDE0823|hypothetical protein:(3R)-hydroxymyristoyl-(acyl-carrier-protein) dehydratase, putative|POSITIVE:POSITIVE|[844730,844880]|151 HSP 1 e-value: 1.0E-80 bit: 299.0 Len: 151 Query Start:1 Query End:151 Subject Strand: POSITIVE Subject Start: 1 Subject End: 151 cggaaaaataccaatcttgattaaatcctttaaagtcgatacgttttcttgtagtccttgtttaatcttggtattttgtgtaaaattatgtgggtacaaatattgattgccatattaagatattatttttatggaagataaggagaaatat cggaaaaataccaatcttgattaaatcctttaaagtcgatacgttttcttgtagtccttgtttaatcttggtattttgtgtaaaattatgtgggtacaaatattgattgccatattaagatattatttttatggaagataaggagaaatat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGGAAAAAUACCAAUCUUGAUUAAAUCCUUUAAAGUCGAUACGUUUUCUUGUAGUCCUUGUUUAAUCUUGGUAUUUUGUGUAAAAUUAUGUGGGUACAAAUAUUGAUUGCCAUAUUAAGAUAUUAUUUUUAUGGAAGAUAAGGAGAAAUAU .((.......))....((((((...........))))))....(((((((((..(((..((...(((((((((...((.((((...(((.((....)).)))....)))))))))))))))...))......)))..)))))))))..... (-21.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAUUUCUCCUUAUCUUCCAUAAAAAUAAUAUCUUAAUAUGGCAAUCAAUAUUUGUACCCACAUAAUUUUACACAAAAUACCAAGAUUAAACAAGGACUACAAGAAAACGUAUCGACUUUAAAGGAUUUAAUCAAGAUUGGUAUUUUUCCG .................(((((...............)))))................................(((((((((((((((((....((.(((........)))))..((....))..)))))))....)))))))))).... (-13.66) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 309 79 33 87 31 NC_002967:TDE0449|5end_ferritin,_putative_507031..507261_NEGATIVE 263 60 11 223 170 NC_002967:TDE2430|5end_bacteriocin_ABC_transporter,_bacteriocin-binding_protein,_putative_2459173..2459403_NEGATIVE 249 56 17 231 191 NC_002967:TDE1521|5end_fumarate_hydratase_1567211..1567441_POSITIVE 248 69 26 63 15 NC_002967:TDE2599|5end_thymidylate_kinase_2633963..2634193_NEGATIVE 244 59 11 146 88 NC_002967:TDE0596|5end_hypothetical_protein_636010..636240_NEGATIVE 238 59 12 228 182 NC_002967:TDE2283|5end_permease,_putative_2320917..2321147_NEGATIVE 237 126 90 109 70 NC_002967:TDE1444|5end_metallo-beta-lactamase_family_protein_1491004..1491234_NEGATIVE 234 90 43 222 172 NC_002967:TDE2681|5end_hypothetical_protein_2721407..2721637_NEGATIVE 234 72 34 174 129 NC_002967:TDE1245|5end_hypothetical_protein_1278894..1279124_NEGATIVE 234 98 51 208 169 NC_002967:TDE0180|5end_hypothetical_protein_213018..213248_NEGATIVE 231 55 11 183 143 NC_002967:TDE1525|5end_hypothetical_protein_1570338..1570568_POSITIVE 231 55 11 137 97 NC_002967:TDE1524|5end_hypothetical_protein_1570292..1570522_POSITIVE 230 69 26 136 92 NC_002967:TDE2760|5end_flagellar_biosynthesis_protein_2815881..2816111_NEGATIVE 229 91 54 75 40 NC_002967:TDE1142|5end_phage_minor_structural_protein,_putative_1179537..1179767_NEGATIVE 226 80 47 225 197 NC_002967:TDE2772|5end_ABC_transporter,_ATP-binding_protein,_truncation_2825646..2825876_POSITIVE 226 79 42 52 13 NC_002967:TDE2102|5end_hypothetical_protein_2127347..2127577_NEGATIVE 222 77 34 170 138 NC_002967:TDE2537|5end_hypothetical_protein_2563266..2563496_POSITIVE 222 84 43 218 173 NC_002967:TDE2478|5end_excinuclease_ABC,_C_subunit_2500873..2501103_POSITIVE 220 110 61 219 176 NC_002967:TDE0261|5end_flavodoxin_294642..294872_POSITIVE 219 82 42 222 160 NC_002967:TDE2676|5end_threonine_synthase_2714285..2714515_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 267 77 32 150 99 NC_002967:TDE2089|3end_signal_recognition_particle_protein_2116232..2116382_NEGATIVE 244 59 11 137 79 NC_002967:TDE0597|3end_hypothetical_protein_636019..636169_NEGATIVE 238 120 80 96 65 NC_002967:TDE1816|3end_hypothetical_protein_1850477..1850627_NEGATIVE 238 82 42 151 116 NC_002967:TDE0108|3end_hypothetical_protein_128787..128937_POSITIVE 231 55 11 86 46 NC_002967:TDE1523|3end_citXG_protein,_putative_1570321..1570471_POSITIVE 230 69 26 139 95 NC_002967:TDE2761|3end_hypothetical_protein_2815878..2816028_NEGATIVE 229 91 54 57 22 NC_002967:TDE1143|3end_hypothetical_protein_1179555..1179705_NEGATIVE 226 80 47 149 121 NC_002967:TDE2771|3end_hypothetical_protein_2825650..2825800_POSITIVE 224 57 11 151 96 NC_002967:TDE0923|3end_ABC_transporter,_ATP-binding/permease_protein_943920..944070_NEGATIVE 215 82 42 133 96 NC_002967:TDE1788|3end_hypothetical_protein_1826628..1826778_NEGATIVE 211 70 21 112 49 NC_002967:TDE0790|3end_30S_ribosomal_protein_S11_817411..817561_POSITIVE 209 59 26 63 34 NC_002967:TDE0480|3end_alpha-L-fucosidase_family_protein_538205..538355_POSITIVE 208 55 21 149 102 NC_002967:TDE0249|3end_flavoredoxin,_putative_283182..283332_POSITIVE 204 49 1 87 42 NC_002967:TDE2265|3end_hypothetical_protein_2302634..2302784_POSITIVE 203 80 40 126 76 NC_002967:TDE0534|3end_hypothetical_protein_577384..577534_POSITIVE 198 60 11 151 96 NC_002967:TDE2514|3end_serine/threonine_protein_phosphatase_family_protein_2539855..2540005_NEGATIVE 196 134 93 130 80 NC_002967:TDE1719|3end_transcriptional_regulator,_MarR_family_1774546..1774696_POSITIVE 196 63 26 96 64 NC_002967:TDE0743|3end_thioredoxin_reductase_779751..779901_POSITIVE 195 70 25 79 15 NC_002967:TDE1106|3end_translation_initiation_factor_IF-2_1138192..1138342_NEGATIVE 195 96 60 44 5 NC_002967:TDE0742|3end_hypothetical_protein_778450..778600_NEGATIVE