Origin IGS: acttcccccaaaatagtgtgtttattttacgccttcaaactaaaaggtataagtttatgcagcgggtacaaagatacttttgggatcttattaagttatagagaaaacaaagatgttataagttccttaagtgtcccgtactttatcgctcgtaaagcacaaggcttagtcctttacaggcaggtattctgacttcgagcttca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaagctcgaagtcagaatacctgcctgtaaaggactaagccttgtgctttacgagcgataaagtacgggacacttaaggaacttataacatctttgttttctctataacttaataagatcccaaaagtatctttgtacccgctgcataaacttataccttttagtttgaaggcgtaaaataaacacactattttgggggaagt Mask Tandem Repeat Region ================================================ acttcccccaaaatagtgtgtttattttacgccttcaaactaaaaggtataagtttatgcagcgggtacaaagatacttttgggatcttattaagttatagagaaaacaaagatgttataagttccttaagtgtcccgtactttatcgctcgtaaagcacaaggcttagtcctttacaggcaggtattctgacttcgagcttca Find is-nt database================================================ Query_seq: TDE0758:TDE0759|TDE0758:TDE0759:iron compound ABC transporter, periplasmic iron compound-binding protein, putative:hypothetical protein:<-<-:799014..799217 204 acttcccccaaaatagtgtgtttattttacgccttcaaactaaaaggtataagtttatgcagcgggtacaaagatacttttgggatcttattaagttatagagaaaacaaagatgttataagttccttaagtgtcccgtactttatcgctcgtaaagcacaaggcttagtcctttacaggcaggtattctgacttcgagcttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE0758:TDE0759|TDE0758:TDE0759:iron compound ABC transporter, periplasmic iron compound-binding protein, putative:hypothetical protein:<-<-:799014..799217 204 acttcccccaaaatagtgtgtttattttacgccttcaaactaaaaggtataagtttatgcagcgggtacaaagatacttttgggatcttattaagttatagagaaaacaaagatgttataagttccttaagtgtcccgtactttatcgctcgtaaagcacaaggcttagtcctttacaggcaggtattctgacttcgagcttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE0758:TDE0759|TDE0758:TDE0759:iron compound ABC transporter, periplasmic iron compound-binding protein, putative:hypothetical protein:<-<-:799014..799217 204 acttcccccaaaatagtgtgtttattttacgccttcaaactaaaaggtataagtttatgcagcgggtacaaagatacttttgggatcttattaagttatagagaaaacaaagatgttataagttccttaagtgtcccgtactttatcgctcgtaaagcacaaggcttagtcctttacaggcaggtattctgacttcgagcttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acttcccccaaaatagtgtgtttattttacgccttcaaactaaaaggtataagtttatgcagcgggtacaaagatacttttgggatcttattaagttatagagaaaacaaagatgttataagttccttaagtgtcccgtactttatcgctcgtaaagcacaaggcttagtcctttacaggcaggtattctgacttcgagcttca Predict ORF larger than 30AA ================================================ Protein_Len: 59 Strand: + Start: 13 End: 189 ............ M V C L F Y A F K L K G I S L C S G Y K D T F G I L L S Y R E N K D V I S S L S V P Y F I A R K A Q G L V L Y R Q V F ............... acttcccccaaaatagtgtgtttattttacgccttcaaactaaaaggtataagtttatgcagcgggtacaaagatacttttgggatcttattaagttatagagaaaacaaagatgttataagttccttaagtgtcccgtactttatcgctcgtaaagcacaaggcttagtcctttacaggcaggtattctgacttcgagcttca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acttcccccaaaatagtgtgtttattttacgccttcaaactaaaaggtataagtttatgcagcgggtacaaagatacttttgggatcttattaagttatagagaaaacaaagatgttataagttccttaagtgtcccgtactttatcgctcgtaaagcacaaggcttagtcctttacaggcaggtattctgacttcgagcttca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TDE0758:TDE0759|TDE0758:TDE0759:iron compound ABC transporter, periplasmic iron compound-binding protein, putative:hypothetical protein:<-<-:799014..799217 204 acttcccccaaaatagtgtgtttattttacgccttcaaactaaaaggtataagtttatgcagcgggtacaaagatacttttgggatcttattaagttatagagaaaacaaagatgttataagttccttaagtgtcccgtactttatcgctcgtaaagcacaaggcttagtcctttacaggcaggtattctgacttcgagcttca Intra-Species Hit: Count: 1 Min: 1 Max: 204 Len: 204 Subject: NC_002967_TDE0758_TDE0759|iron compound ABC transporter, periplasmic iron compound-binding protein, putative:hypothetical protein|NEGATIVE:NEGATIVE|[799014,799217]|204 HSP 1 e-value: 1.0E-112 bit: 404.0 Len: 204 Query Start:1 Query End:204 Subject Strand: POSITIVE Subject Start: 1 Subject End: 204 acttcccccaaaatagtgtgtttattttacgccttcaaactaaaaggtataagtttatgcagcgggtacaaagatacttttgggatcttattaagttatagagaaaacaaagatgttataagttccttaagtgtcccgtactttatcgctcgtaaagcacaaggcttagtcctttacaggcaggtattctgacttcgagcttca acttcccccaaaatagtgtgtttattttacgccttcaaactaaaaggtataagtttatgcagcgggtacaaagatacttttgggatcttattaagttatagagaaaacaaagatgttataagttccttaagtgtcccgtactttatcgctcgtaaagcacaaggcttagtcctttacaggcaggtattctgacttcgagcttca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACUUCCCCCAAAAUAGUGUGUUUAUUUUACGCCUUCAAACUAAAAGGUAUAAGUUUAUGCAGCGGGUACAAAGAUACUUUUGGGAUCUUAUUAAGUUAUAGAGAAAACAAAGAUGUUAUAAGUUCCUUAAGUGUCCCGUACUUUAUCGCUCGUAAAGCACAAGGCUUAGUCCUUUACAGGCAGGUAUUCUGACUUCGAGCUUCA ................(((((((..((((..((((........))))..))))..((((.(((((((((...(((((((..((((.(((((...(((..............)))...)))))))))..)))))))..)))))....))))))))))))))).(((((((((...(((......)))....))))..)))))... (-46.04) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAGCUCGAAGUCAGAAUACCUGCCUGUAAAGGACUAAGCCUUGUGCUUUACGAGCGAUAAAGUACGGGACACUUAAGGAACUUAUAACAUCUUUGUUUUCUCUAUAACUUAAUAAGAUCCCAAAAGUAUCUUUGUACCCGCUGCAUAAACUUAUACCUUUUAGUUUGAAGGCGUAAAAUAAACACACUAUUUUGGGGGAAGU .....((((((((.((......(((.(((((((.((........)).))))))).))).....((((((((((...((((.((((.....(((((.(((........))).....))))).....)))).))))))).)))).)))......(((..(((((......)))))..)))..........))))))))))...... (-42.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 827 80 31 171 122 NC_002967:TDE0758|5end_iron_compound_ABC_transporter,_periplasmic_iron_compound-binding_protein,_putative_798923..799153_NEGATIVE 348 174 131 158 115 NC_002967:TDE1825|5end_hypothetical_protein_1858344..1858574_NEGATIVE 322 180 132 174 123 NC_002967:TDE0672|5end_hypothetical_protein_711110..711340_POSITIVE 319 168 121 231 178 NC_002967:TDE0327|5end_CRISPR-associated_SAG0894_family_protein_360881..361111_POSITIVE 308 169 125 63 13 NC_002967:TDE2660|5end_hypothetical_protein_2697517..2697747_NEGATIVE 302 177 134 172 106 NC_002967:TDE0523|5end_hypothetical_protein_569831..570061_NEGATIVE 299 167 121 114 72 NC_002967:TDE2662|5end_lipoprotein,_putative_2698650..2698880_NEGATIVE 289 190 145 226 171 NC_002967:TDE1550|5end_diphosphate--fructose-6-phosphate_1-phosphotransferase_1592747..1592977_POSITIVE 289 200 152 172 123 NC_002967:TDE0466|5end_hypothetical_protein_518854..519084_NEGATIVE 287 198 161 124 92 NC_002967:TDE2057|5end_hypothetical_protein_2085343..2085573_NEGATIVE 284 177 131 186 128 NC_002967:TDE1104|5end_tRNA_pseudouridine_synthase_B_1137786..1138016_NEGATIVE 281 199 156 43 5 NC_002967:TDE2056|5end_outer_membrane_hemin-binding_protein_A_2085070..2085300_NEGATIVE 280 184 142 63 33 NC_002967:TDE0136|5end_hypothetical_protein_156287..156517_POSITIVE 270 172 133 70 18 NC_002967:TDE0294|5end_TrkA_domain_protein_324210..324440_NEGATIVE 269 184 144 138 82 NC_002967:TDE1285|5end_flagellar_motor_switch_protein_FliG_,_putative_1320924..1321154_POSITIVE 268 166 122 221 176 NC_002967:TDE1225|5end_zinc_ABC_transporter,_ATP-binding_protein_1259518..1259748_NEGATIVE 267 180 132 202 152 NC_002967:TDE1403|5end_ABC_transporter,_ATP-binding_protein_1446503..1446733_NEGATIVE 267 165 122 64 24 NC_002967:TDE0169|5end_methyl-accepting_chemotaxis_domain_protein_198792..199022_NEGATIVE 266 191 151 52 13 NC_002967:TDE1290|5end_hypothetical_protein_1327399..1327629_POSITIVE 265 182 146 90 54 NC_002967:TDE0625|5end_ABC_transporter,_ATP-binding_protein_657253..657483_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 322 180 132 62 11 NC_002967:TDE0671|3end_PIN_domain_protein_711078..711228_POSITIVE 308 169 125 56 6 NC_002967:TDE2661|3end_hypothetical_protein_2697524..2697674_NEGATIVE 299 167 121 148 106 NC_002967:TDE2663|3end_isoleucyl-tRNA_synthetase_2698616..2698766_NEGATIVE 287 198 161 113 81 NC_002967:TDE2058|3end_hypothetical_protein_2085354..2085504_NEGATIVE 283 200 152 74 24 NC_002967:TDE0591|3end_acetyl-CoA_carboxylase,_biotin_carboxylase_630033..630183_NEGATIVE 280 184 142 73 43 NC_002967:TDE0135|3end_pyridine_nucleotide-disulphide_oxidoreductase_family_protein_156377..156527_POSITIVE 276 190 145 116 63 NC_002967:TDE1004|3end_flagellar_filament_core_protein_1029276..1029426_NEGATIVE 271 200 157 83 40 NC_002967:TDE2487|3end_acetyltransferase,_GNAT_family_2510393..2510543_POSITIVE 271 174 135 41 4 NC_002967:TDE0218|3end_hypothetical_protein_253367..253517_POSITIVE 270 172 133 60 8 NC_002967:TDE0295|3end_DNA_gyrase,_A_subunit_324220..324370_NEGATIVE 269 184 144 57 1 NC_002967:TDE1284|3end_methyl-accepting_chemotaxis_protein,_putative_1320923..1321073_POSITIVE 268 199 157 116 86 NC_002967:TDE1977|3end_hypothetical_protein_1991348..1991498_POSITIVE 266 191 151 83 44 NC_002967:TDE1289|3end_hypothetical_protein_1327510..1327660_POSITIVE 265 182 146 97 61 NC_002967:TDE0624|3end_precorrin-6X_reductase,_putative_657340..657490_POSITIVE 264 184 141 144 102 NC_002967:TDE1499|3end_adenylosuccinate_lyase,_putative_1546788..1546938_POSITIVE 260 158 117 64 19 NC_002967:TDE1395|3end_glycosyl_hydrolase,_family_57_1435678..1435828_POSITIVE 259 104 61 110 55 NC_002967:TDE0489|3end_hypothetical_protein_545156..545306_NEGATIVE 258 200 152 71 22 NC_002967:TDE1299|3end_hypothetical_protein_1335417..1335567_NEGATIVE 255 174 142 51 18 NC_002967:TDE0751|3end_magnesium_chelatase,_subunit_D/I_family_790122..790272_NEGATIVE 253 180 133 137 81 NC_002967:TDE0630|3end_sigma_factor_regulatory_protein,_putative_665371..665521_POSITIVE