Origin IGS: tataggagaaaattaaaatgaaaaaattacaaatttctgtaaaaaagcacttgacatattgcaaaagtatggtacacttaaagtaaggtcttttgaaaagcctaaaaatatttaagtttaggtgtaggagcctaaacaaaggagttgttttatgaaagatttattgaacaaagggtttcagcctgtgtctgaaacagagatggtaatggttgagggtggttgttcccatcctttttgttttactgattgtacagccgctggtacagacagttcgaatgataattcattacttgaactgttaaagaattttaagattaaaagggatgatgtaacaatagattgggaaggggttcattttgatttttcaaactttgatttagatattggtggaattggaggtggtcgagccggaataaaagttacaataccgctttaaatattttgatttaattcgacaatgggagtttaaatgaaaaataaatttctaataatgttgttgtgtgtatttatattttcttgctcaacaatgaatagtaagcatgcagctaaaaataaactaccgatttatgtattatcaggaaaagtgcggatagaacttatatgtcaacattctatatatcatcatcaaaatgattctataattaaaattcataggcgaggtgtaaataatgattcactatttgaaaaaagaataatgttttttacggcgggtgagatgtttgatcaatcattggtattttatgactctattatagctaaagttgttgcagaggagcaacctgctgaaataatgtgtagcggaaaatattataaaattactcctgataacaaatctgggatagttttttctttttataaaaattaagcttaacataagatgcaaatagtgttataatgtattttgaggagagatc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gatctctcctcaaaatacattataacactatttgcatcttatgttaagcttaatttttataaaaagaaaaaactatcccagatttgttatcaggagtaattttataatattttccgctacacattatttcagcaggttgctcctctgcaacaactttagctataatagagtcataaaataccaatgattgatcaaacatctcacccgccgtaaaaaacattattcttttttcaaatagtgaatcattatttacacctcgcctatgaattttaattatagaatcattttgatgatgatatatagaatgttgacatataagttctatccgcacttttcctgataatacataaatcggtagtttatttttagctgcatgcttactattcattgttgagcaagaaaatataaatacacacaacaacattattagaaatttatttttcatttaaactcccattgtcgaattaaatcaaaatatttaaagcggtattgtaacttttattccggctcgaccacctccaattccaccaatatctaaatcaaagtttgaaaaatcaaaatgaaccccttcccaatctattgttacatcatcccttttaatcttaaaattctttaacagttcaagtaatgaattatcattcgaactgtctgtaccagcggctgtacaatcagtaaaacaaaaaggatgggaacaaccaccctcaaccattaccatctctgtttcagacacaggctgaaaccctttgttcaataaatctttcataaaacaactcctttgtttaggctcctacacctaaacttaaatatttttaggcttttcaaaagaccttactttaagtgtaccatacttttgcaatatgtcaagtgcttttttacagaaatttgtaattttttcattttaattttctcctata Mask Tandem Repeat Region ================================================ tataggagaaaattaaaatgaaaaaattacaaatttctgtaaaaaagcacttgacatattgcaaaagtatggtacacttaaagtaaggtcttttgaaaagcctaaaaatatttaagtttaggtgtaggagcctaaacaaaggagttgttttatgaaagatttattgaacaaagggtttcagcctgtgtctgaaacagagatggtaatggttgagggtggttgttcccatcctttttgttttactgattgtacagccgctggtacagacagttcgaatgataattcattacttgaactgttaaagaattttaagattaaaagggatgatgtaacaatagattgggaaggggttcattttgatttttcaaactttgatttagatattggtggaattggaggtggtcgagccggaataaaagttacaataccgctttaaatattttgatttaattcgacaatgggagtttaaatgaaaaataaatttctaataatgttgttgtgtgtatttatattttcttgctcaacaatgaatagtaagcatgcagctaaaaataaactaccgatttatgtattatcaggaaaagtgcggatagaacttatatgtcaacattctatatatcatcatcaaaatgattctataattaaaattcataggcgaggtgtaaataatgattcactatttgaaaaaagaataatgttttttacggcgggtgagatgtttgatcaatcattggtattttatgactctattatagctaaagttgttgcagaggagcaacctgctgaaataatgtgtagcggaaaatattataaaattactcctgataacaaatctgggatagttttttctttttataaaaattaagcttaacataagatgcaaatagtgttataatgtattttgaggagagatc Find is-nt database================================================ Query_seq: TDE0717:TDE0718|TDE0717:TDE0718:hypothetical protein:hypothetical protein:->->:752441..753354 914 tataggagaaaattaaaatgaaaaaattacaaatttctgtaaaaaagcacttgacatattgcaaaagtatggtacacttaaagtaaggtcttttgaaaagcctaaaaatatttaagtttaggtgtaggagcctaaacaaaggagttgttttatgaaagatttattgaacaaagggtttcagcctgtgtctgaaacagagatggtaatggttgagggtggttgttcccatcctttttgttttactgattgtacagccgctggtacagacagttcgaatgataattcattacttgaactgttaaagaattttaagattaaaagggatgatgtaacaatagattgggaaggggttcattttgatttttcaaactttgatttagatattggtggaattggaggtggtcgagccggaataaaagttacaataccgctttaaatattttgatttaattcgacaatgggagtttaaatgaaaaataaatttctaataatgttgttgtgtgtatttatattttcttgctcaacaatgaatagtaagcatgcagctaaaaataaactaccgatttatgtattatcaggaaaagtgcggatagaacttatatgtcaacattctatatatcatcatcaaaatgattctataattaaaattcataggcgaggtgtaaataatgattcactatttgaaaaaagaataatgttttttacggcgggtgagatgtttgatcaatcattggtattttatgactctattatagctaaagttgttgcagaggagcaacctgctgaaataatgtgtagcggaaaatattataaaattactcctgataacaaatctgggatagttttttctttttataaaaattaagcttaacataagatgcaaatagtgttataatgtattttgaggagagatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE0717:TDE0718|TDE0717:TDE0718:hypothetical protein:hypothetical protein:->->:752441..753354 914 tataggagaaaattaaaatgaaaaaattacaaatttctgtaaaaaagcacttgacatattgcaaaagtatggtacacttaaagtaaggtcttttgaaaagcctaaaaatatttaagtttaggtgtaggagcctaaacaaaggagttgttttatgaaagatttattgaacaaagggtttcagcctgtgtctgaaacagagatggtaatggttgagggtggttgttcccatcctttttgttttactgattgtacagccgctggtacagacagttcgaatgataattcattacttgaactgttaaagaattttaagattaaaagggatgatgtaacaatagattgggaaggggttcattttgatttttcaaactttgatttagatattggtggaattggaggtggtcgagccggaataaaagttacaataccgctttaaatattttgatttaattcgacaatgggagtttaaatgaaaaataaatttctaataatgttgttgtgtgtatttatattttcttgctcaacaatgaatagtaagcatgcagctaaaaataaactaccgatttatgtattatcaggaaaagtgcggatagaacttatatgtcaacattctatatatcatcatcaaaatgattctataattaaaattcataggcgaggtgtaaataatgattcactatttgaaaaaagaataatgttttttacggcgggtgagatgtttgatcaatcattggtattttatgactctattatagctaaagttgttgcagaggagcaacctgctgaaataatgtgtagcggaaaatattataaaattactcctgataacaaatctgggatagttttttctttttataaaaattaagcttaacataagatgcaaatagtgttataatgtattttgaggagagatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE0717:TDE0718|TDE0717:TDE0718:hypothetical protein:hypothetical protein:->->:752441..753354 914 tataggagaaaattaaaatgaaaaaattacaaatttctgtaaaaaagcacttgacatattgcaaaagtatggtacacttaaagtaaggtcttttgaaaagcctaaaaatatttaagtttaggtgtaggagcctaaacaaaggagttgttttatgaaagatttattgaacaaagggtttcagcctgtgtctgaaacagagatggtaatggttgagggtggttgttcccatcctttttgttttactgattgtacagccgctggtacagacagttcgaatgataattcattacttgaactgttaaagaattttaagattaaaagggatgatgtaacaatagattgggaaggggttcattttgatttttcaaactttgatttagatattggtggaattggaggtggtcgagccggaataaaagttacaataccgctttaaatattttgatttaattcgacaatgggagtttaaatgaaaaataaatttctaataatgttgttgtgtgtatttatattttcttgctcaacaatgaatagtaagcatgcagctaaaaataaactaccgatttatgtattatcaggaaaagtgcggatagaacttatatgtcaacattctatatatcatcatcaaaatgattctataattaaaattcataggcgaggtgtaaataatgattcactatttgaaaaaagaataatgttttttacggcgggtgagatgtttgatcaatcattggtattttatgactctattatagctaaagttgttgcagaggagcaacctgctgaaataatgtgtagcggaaaatattataaaattactcctgataacaaatctgggatagttttttctttttataaaaattaagcttaacataagatgcaaatagtgttataatgtattttgaggagagatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tataggagaaaattaaaatgaaaaaattacaaatttctgtaaaaaagcacttgacatattgcaaaagtatggtacacttaaagtaaggtcttttgaaaagcctaaaaatatttaagtttaggtgtaggagcctaaacaaaggagttgttttatgaaagatttattgaacaaagggtttcagcctgtgtctgaaacagagatggtaatggttgagggtggttgttcccatcctttttgttttactgattgtacagccgctggtacagacagttcgaatgataattcattacttgaactgttaaagaattttaagattaaaagggatgatgtaacaatagattgggaaggggttcattttgatttttcaaactttgatttagatattggtggaattggaggtggtcgagccggaataaaagttacaataccgctttaaatattttgatttaattcgacaatgggagtttaaatgaaaaataaatttctaataatgttgttgtgtgtatttatattttcttgctcaacaatgaatagtaagcatgcagctaaaaataaactaccgatttatgtattatcaggaaaagtgcggatagaacttatatgtcaacattctatatatcatcatcaaaatgattctataattaaaattcataggcgaggtgtaaataatgattcactatttgaaaaaagaataatgttttttacggcgggtgagatgtttgatcaatcattggtattttatgactctattatagctaaagttgttgcagaggagcaacctgctgaaataatgtgtagcggaaaatattataaaattactcctgataacaaatctgggatagttttttctttttataaaaattaagcttaacataagatgcaaatagtgttataatgtattttgaggagagatc Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 210 End: 299 ................................................................................................................................................................................................................. M R V V V P I L F V L L I V Q P L V Q T V R M I I H Y L N C ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 152 End: 433 ....................................................................................................................................................... M K D L L N K G F Q P V S E T E M V M V E G G C S H P F C F T D C T A A G T D S S N D N S L L E L L K N F K I K R D D V ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 470 End: 862 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M K N K F L I M L L C V F I F S C S T M N S K H A A K N K L P I Y V L S G K V R I E L I C Q H S I Y H H Q N D S I I K I .................................................... Protein_Len: 51 Strand: - Start: 731 End: 883 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Q Y K I V R N Y S F N N C L L L R S F Y H T A S F I I F N S R I V F R P Y N K R K I F I L S L M L H M ............................... Protein_Len: 32 Strand: - Start: 578 End: 673 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. S F H P Y F K Y T L M R Y I M M L I I R Y N F N M P S T Y I I M ................................................................................................................................................................................................................................................. Protein_Len: 32 Strand: - Start: 469 End: 564 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... I F F L N R I I N N H T N I N E Q E V I F L L C A A L F L S G M .............................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 33 Strand: - Start: 380 End: 478 ........................................................................................................................................................................................................................................................................................................................................................................................... I N T S N S T T S G S Y F N C Y R K L Y K S K I R C H S N L H F M .................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 52 Strand: - Start: 181 End: 336 .................................................................................................................................................................................... G T D S V S I T I T S P P Q E W G K Q K V S Q V A A P V S L E F S L E N S S S N F F K L I L L S S T V M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 40 Strand: - Start: 164 End: 283 ................................................................................................................................................................... Q V F P K L R H R F C L H Y H N L T T T G M R K T K S I T C G S T C V T R I I M ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 32 Strand: - Start: 16 End: 111 ............... F S F I V F K Q L F A S S M N C F Y P V S L T L D K S F G L F M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 53 Strand: - Start: 2 End: 160 . Y S F I L I F F N C I E T F F C K V Y Q L L I T C K F Y P R K F L R F I N L N L H L L R F L P T T K H F M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. tataggagaaaattaaaatgaaaaaattacaaatttctgtaaaaaagcacttgacatattgcaaaagtatggtacacttaaagtaaggtcttttgaaaagcctaaaaatatttaagtttaggtgtaggagcctaaacaaaggagttgttttatgaaagatttattgaacaaagggtttcagcctgtgtctgaaacagagatggtaatggttgagggtggttgttcccatcctttttgttttactgattgtacagccgctggtacagacagttcgaatgataattcattacttgaactgttaaagaattttaagattaaaagggatgatgtaacaatagattgggaaggggttcattttgatttttcaaactttgatttagatattggtggaattggaggtggtcgagccggaataaaagttacaataccgctttaaatattttgatttaattcgacaatgggagtttaaatgaaaaataaatttctaataatgttgttgtgtgtatttatattttcttgctcaacaatgaatagtaagcatgcagctaaaaataaactaccgatttatgtattatcaggaaaagtgcggatagaacttatatgtcaacattctatatatcatcatcaaaatgattctataattaaaattcataggcgaggtgtaaataatgattcactatttgaaaaaagaataatgttttttacggcgggtgagatgtttgatcaatcattggtattttatgactctattatagctaaagttgttgcagaggagcaacctgctgaaataatgtgtagcggaaaatattataaaattactcctgataacaaatctgggatagttttttctttttataaaaattaagcttaacataagatgcaaatagtgttataatgtattttgaggagagatc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 90 Start: 867 End: 896 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CTTAACATAAGATGCAAATAGTGTTATAAT.................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tataggagaaaattaaaatgaaaaaattacaaatttctgtaaaaaagcacttgacatattgcaaaagtatggtacacttaaagtaaggtcttttgaaaagcctaaaaatatttaagtttaggtgtaggagcctaaacaaaggagttgttttatgaaagatttattgaacaaagggtttcagcctgtgtctgaaacagagatggtaatggttgagggtggttgttcccatcctttttgttttactgattgtacagccgctggtacagacagttcgaatgataattcattacttgaactgttaaagaattttaagattaaaagggatgatgtaacaatagattgggaaggggttcattttgatttttcaaactttgatttagatattggtggaattggaggtggtcgagccggaataaaagttacaataccgctttaaatattttgatttaattcgacaatgggagtttaaatgaaaaataaatttctaataatgttgttgtgtgtatttatattttcttgctcaacaatgaatagtaagcatgcagctaaaaataaactaccgatttatgtattatcaggaaaagtgcggatagaacttatatgtcaacattctatatatcatcatcaaaatgattctataattaaaattcataggcgaggtgtaaataatgattcactatttgaaaaaagaataatgttttttacggcgggtgagatgtttgatcaatcattggtattttatgactctattatagctaaagttgttgcagaggagcaacctgctgaaataatgtgtagcggaaaatattataaaattactcctgataacaaatctgggatagttttttctttttataaaaattaagcttaacataagatgcaaatagtgttataatgtattttgaggagagatc Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 72 HP_score: -2.6 Tail_Score: -5.48054 Start: 27 End: 42 Full_Region: atgtcaagtgctttt ttacag aaat ttgtaa ttttttcattttaat ..........................ttacagaaatttgtaa........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 85 HP_score: -4.8 Tail_Score: -5.50723 Start: 214 End: 231 Full_Region: GATGGTAATGGTTGA GGGTGG TTGTTC CCATCC TTTTTGTTTTACTGA .....................................................................................................................................................................................................................GGGTGGTTGTTCCCATCC........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: TDE0717:TDE0718|TDE0717:TDE0718:hypothetical protein:hypothetical protein:->->:752441..753354 914 tataggagaaaattaaaatgaaaaaattacaaatttctgtaaaaaagcacttgacatattgcaaaagtatggtacacttaaagtaaggtcttttgaaaagcctaaaaatatttaagtttaggtgtaggagcctaaacaaaggagttgttttatgaaagatttattgaacaaagggtttcagcctgtgtctgaaacagagatggtaatggttgagggtggttgttcccatcctttttgttttactgattgtacagccgctggtacagacagttcgaatgataattcattacttgaactgttaaagaattttaagattaaaagggatgatgtaacaatagattgggaaggggttcattttgatttttcaaactttgatttagatattggtggaattggaggtggtcgagccggaataaaagttacaataccgctttaaatattttgatttaattcgacaatgggagtttaaatgaaaaataaatttctaataatgttgttgtgtgtatttatattttcttgctcaacaatgaatagtaagcatgcagctaaaaataaactaccgatttatgtattatcaggaaaagtgcggatagaacttatatgtcaacattctatatatcatcatcaaaatgattctataattaaaattcataggcgaggtgtaaataatgattcactatttgaaaaaagaataatgttttttacggcgggtgagatgtttgatcaatcattggtattttatgactctattatagctaaagttgttgcagaggagcaacctgctgaaataatgtgtagcggaaaatattataaaattactcctgataacaaatctgggatagttttttctttttataaaaattaagcttaacataagatgcaaatagtgttataatgtattttgaggagagatc Intra-Species Hit: Count: 2 Min: 47 Max: 914 Len: 868 Subject: NC_002967_TDE0717_TDE0718|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[752441,753354]|914 HSP 1 e-value: 0.0 bit: 1649.0 Len: 868 Query Start:47 Query End:914 Subject Strand: POSITIVE Subject Start: 47 Subject End: 914 ..............................................gcacttgacatattgcaaaagtatggtacacttaaagtaaggtcttttgaaaagcctaaaaatatttaagtttaggtgtaggagcctaaacaaaggagttgttttatgaaagatttattgaacaaagggtttcagcctgtgtctgaaacagagatggtaatggttgagggtggttgttcccatcctttttgttttactgattgtacagccgctggtacagacagttcgaatgataattcattacttgaactgttaaagaattttaagattaaaagggatgatgtaacaatagattgggaaggggttcattttgatttttcaaactttgatttagatattggtggaattggaggtggtcgagccggaataaaagttacaataccgctttaaatattttgatttaattcgacaatgggagtttaaatgaaaaataaatttctaataatgttgttgtgtgtatttatattttcttgctcaacaatgaatagtaagcatgcagctaaaaataaactaccgatttatgtattatcaggaaaagtgcggatagaacttatatgtcaacattctatatatcatcatcaaaatgattctataattaaaattcataggcgaggtgtaaataatgattcactatttgaaaaaagaataatgttttttacggcgggtgagatgtttgatcaatcattggtattttatgactctattatagctaaagttgttgcagaggagcaacctgctgaaataatgtgtagcggaaaatattataaaattactcctgataacaaatctgggatagnnnnnnnnnnnnataaaaattaagcttaacataagatgcaaatagtgttataatgtattttgaggagagatc ..............................................gcacttgacatattgcaaaagtatggtacacttaaagtaaggtcttttgaaaagcctaaaaatatttaagtttaggtgtaggagcctaaacaaaggagttgttttatgaaagatttattgaacaaagggtttcagcctgtgtctgaaacagagatggtaatggttgagggtggttgttcccatcctttttgttttactgattgtacagccgctggtacagacagttcgaatgataattcattacttgaactgttaaagaattttaagattaaaagggatgatgtaacaatagattgggaaggggttcattttgatttttcaaactttgatttagatattggtggaattggaggtggtcgagccggaataaaagttacaataccgctttaaatattttgatttaattcgacaatgggagtttaaatgaaaaataaatttctaataatgttgttgtgtgtatttatattttcttgctcaacaatgaatagtaagcatgcagctaaaaataaactaccgatttatgtattatcaggaaaagtgcggatagaacttatatgtcaacattctatatatcatcatcaaaatgattctataattaaaattcataggcgaggtgtaaataatgattcactatttgaaaaaagaataatgttttttacggcgggtgagatgtttgatcaatcattggtattttatgactctattatagctaaagttgttgcagaggagcaacctgctgaaataatgtgtagcggaaaatattataaaattactcctgataacaaatctgggatagttttttctttttataaaaattaagcttaacataagatgcaaatagtgttataatgtattttgaggagagatc Subject: NC_002967_TDE0721_TDE0722|hypothetical protein:lipoprotein, putative|POSITIVE:POSITIVE|[758277,758658]|382 HSP 1 e-value: 2.0E-59 bit: 232.0 Len: 169 Query Start:48 Query End:216 Subject Strand: POSITIVE Subject Start: 26 Subject End: 194 ...............................................cacttgacatattgcaaaagtatggtacacttaaagtaaggtcttttgaaaagcctaaaaatatttaagtttaggtgtaggagcctaaacaaaggagttgttttatgaaagatttattgaacaaagggtttcagcctgtgtctgaaacagagatggtaatggttgaggg.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ...............................................cacttgatatattgtaaaaatatggtatagttaaagtaaggtctttttaaaagactaaaaatatttaagtttaggtctagcgtcctaaacaaaggagttgttttatgaaagatttattgaacaatgggtttcagcctgtgtctgaaacggagatggtaatggttgaggg.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCACUUGACAUAUUGCAAAAGUAUGGUACACUUAAAGUAAGGUCUUUUGAAAAGCCUAAAAAUAUUUAAGUUUAGGUGUAGGAGCCUAAACAAAGGAGUUGUUUUAUGAAAGAUUUAUUGAACAAAGGGUUUCAGCCUGUGUCUGAAACAGAGAUGGUAAUGGUUGAGGGUGGUUGUUCCCAUCCUUUUUGUUUUACUGAUUGUACAGCCGCUGGUACAGACAGUUCGAAUGAUAAUUCAUUACUUGAACUGUUAAAGAAUUUUAAGAUUAAAAGGGAUGAUGUAACAAUAGAUUGGGAAGGGGUUCAUUUUGAUUUUUCAAACUUUGAUUUAGAUAUUGGUGGAAUUGGAGGUGGUCGAGCCGGAAUAAAAGUUACAAUACCGCUUUAAAUAUUUUGAUUUAAUUCGACAAUGGGAGUUUAAAUGAAAAAUAAAUUUCUAAUAAUGUUGUUGUGUGUAUUUAUAUUUUCUUGCUCAACAAUGAAUAGUAAGCAUGCAGCUAAAAAUAAACUACCGAUUUAUGUAUUAUCAGGAAAAGUGCGGAUAGAACUUAUAUGUCAACAUUCUAUAUAUCAUCAUCAAAAUGAUUCUAUAAUUAAAAUUCAUAGGCGAGGUGUAAAUAAUGAUUCACUAUUUGAAAAAAGAAUAAUGUUUUUUACGGCGGGUGAGAUGUUUGAUCAAUCAUUGGUAUUUUAUGACUCUAUUAUAGCUAAAGUUGUUGCAGAGGAGCAACCUGCUGAAAUAAUGUGUAGCGGAAAAUAUUAUAAAAUUACUCCUGAUAACAAAUCUGGGAUAGUUUUUUCUUUUUAUAAAAAUUAAGCUUAACAUAAGAUGCAAAUAGUGUUAUAAUGUAUUUUGAGGAGAGAUC ...(((..((((((.....)))))).....(((((((((..(((((.((..((((.(((.(((((((..((((((((......))))))))...(((((..(..(((.((.((((.((..((((.....(((((.(((((((..((((((.....(((((.....((((((((......))))))))......))))).....((((.(((((......((((((((((((((....))))...))))))))))...((((((((((((((.....(((((((.....(((((.((.((..((((((..(((((((((((......((((.((...((((((((.....((((((((..(((..........))).....))))))))...((((((..........((((.((((((((((.........))))))))))....))))(((((((((..((((...((((((.....))).)))..)))))))))))))..))))))..))))))))...)).))))....))))))).))))..)))))).....))..)).)))))....)))))))....)))))......)))))))))...)))).).))))........((((.....))))..........))))))..)).))))))))))))))..)).)))).)).)))....)..)))))......((((..((((((.((((((.....))).))).))))))...))))...)))))))..(((((((..((.(((.....))).))..)))))))...............))).))))..)).)))))(((.....))).......)))))))))..)))... (-199.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUCUCUCCUCAAAAUACAUUAUAACACUAUUUGCAUCUUAUGUUAAGCUUAAUUUUUAUAAAAAGAAAAAACUAUCCCAGAUUUGUUAUCAGGAGUAAUUUUAUAAUAUUUUCCGCUACACAUUAUUUCAGCAGGUUGCUCCUCUGCAACAACUUUAGCUAUAAUAGAGUCAUAAAAUACCAAUGAUUGAUCAAACAUCUCACCCGCCGUAAAAAACAUUAUUCUUUUUUCAAAUAGUGAAUCAUUAUUUACACCUCGCCUAUGAAUUUUAAUUAUAGAAUCAUUUUGAUGAUGAUAUAUAGAAUGUUGACAUAUAAGUUCUAUCCGCACUUUUCCUGAUAAUACAUAAAUCGGUAGUUUAUUUUUAGCUGCAUGCUUACUAUUCAUUGUUGAGCAAGAAAAUAUAAAUACACACAACAACAUUAUUAGAAAUUUAUUUUUCAUUUAAACUCCCAUUGUCGAAUUAAAUCAAAAUAUUUAAAGCGGUAUUGUAACUUUUAUUCCGGCUCGACCACCUCCAAUUCCACCAAUAUCUAAAUCAAAGUUUGAAAAAUCAAAAUGAACCCCUUCCCAAUCUAUUGUUACAUCAUCCCUUUUAAUCUUAAAAUUCUUUAACAGUUCAAGUAAUGAAUUAUCAUUCGAACUGUCUGUACCAGCGGCUGUACAAUCAGUAAAACAAAAAGGAUGGGAACAACCACCCUCAACCAUUACCAUCUCUGUUUCAGACACAGGCUGAAACCCUUUGUUCAAUAAAUCUUUCAUAAAACAACUCCUUUGUUUAGGCUCCUACACCUAAACUUAAAUAUUUUUAGGCUUUUCAAAAGACCUUACUUUAAGUGUACCAUACUUUUGCAAUAUGUCAAGUGC .................((((...(((....(((((((((.((..((((((((......................................((((((..((((((((.((((......(((.....(((((((..(((((......))))).......(((..(((((((..............((((((..((............(((((......(((((((.........)))))))..........((((..........(((((((((...(((((......(((((.(((((.(((((..((((..(((.((((((...((((.......((((((((..........((((((.......)))))).(((((((........).)))))).........................)))))))).....((((((..((((((..((........)).)))))).))))))......))))....).))))).)))..))))...............................(((...(((((....))))).)))............)))))))))))))))....)))))...)))))))))....(((((((....(((((....))))).))))))).))))...)))))))..))))))...........(((.(((......))).))).............)))))))..)).)....))))))).....))).....))))..)).))))))..))))))..(((((((........)))))))..........))))))))..)).)))).........((((((...)))))).)))))..)))..)))). (-144.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 338 170 122 76 31 NC_002967:TDE2170|5end_hypothetical_protein_2201749..2201979_NEGATIVE 333 179 134 153 110 NC_002967:TDE2619|5end_RNA_methyltransferase,_TrmA_family,_putative_2660280..2660510_POSITIVE 326 239 191 78 38 NC_002967:TDE2342|5end_1-deoxy-D-xylulose_5-phosphate_reductoisomerase_2380383..2380613_NEGATIVE 323 756 711 205 158 NC_002967:TDE2528|5end_hypothetical_protein_2556912..2557142_NEGATIVE 317 739 709 149 114 NC_002967:TDE0073|5end_riboflavin_biosynthesis_protein_RibF,_putative_84082..84312_NEGATIVE 311 175 133 128 96 NC_002967:TDE1847|5end_hypothetical_protein_1875664..1875894_POSITIVE 309 216 171 211 158 NC_002967:TDE2259|5end_hypothetical_protein_2297237..2297467_NEGATIVE 306 219 175 159 111 NC_002967:TDE2632|5end_peptide_ABC_transporter,_ATP-binding_protein,_putative_2672650..2672880_POSITIVE 306 190 143 148 103 NC_002967:TDE0998|5end_hypothetical_protein_1025202..1025432_POSITIVE 302 362 321 218 172 NC_002967:TDE0468|5end_hypothetical_protein_521273..521503_NEGATIVE 299 179 132 172 132 NC_002967:TDE2601|5end_surface_antigen,_putative_2638490..2638720_POSITIVE 293 166 124 64 19 NC_002967:TDE2025|5end_hypothetical_protein_2051440..2051670_NEGATIVE 292 760 717 57 10 NC_002967:TDE1856|5end_radical_SAM_domain_protein_1883133..1883363_POSITIVE 292 170 127 67 31 NC_002967:TDE0204|5end_glutamyl-tRNA_synthetase_236968..237198_POSITIVE 290 218 175 155 91 NC_002967:TDE1254|5end_Smr_domain_protein_1288411..1288641_POSITIVE 290 181 141 229 186 NC_002967:TDE1043|5end_hypothetical_protein_1070945..1071175_POSITIVE 287 388 343 94 45 NC_002967:TDE1380|5end_hypothetical_protein_1421284..1421514_NEGATIVE 286 386 345 68 17 NC_002967:TDE1664|5end_hypothetical_protein_1717862..1718092_POSITIVE 285 220 172 78 26 NC_002967:TDE0522|5end_hypothetical_protein_568499..568729_POSITIVE 280 165 127 78 37 NC_002967:TDE1866|5end_hypothetical_protein_1892354..1892584_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 338 170 122 48 3 NC_002967:TDE2171|3end_hypothetical_protein_2201777..2201927_NEGATIVE 333 179 134 140 97 NC_002967:TDE2618|3end_PHP_domain_protein_2660347..2660497_POSITIVE 326 239 191 78 38 NC_002967:TDE2343|3end_phosphatidate_cytidylyltransferase_2380383..2380533_NEGATIVE 323 756 711 139 92 NC_002967:TDE2529|3end_hypothetical_protein_2556978..2557128_NEGATIVE 317 739 709 75 40 NC_002967:TDE0074|3end_amino_acid_permease_family_protein_84156..84306_NEGATIVE 292 760 717 57 10 NC_002967:TDE1855|3end_hypothetical_protein_1883213..1883363_POSITIVE 292 170 127 77 41 NC_002967:TDE0203|3end_hypothetical_protein_237058..237208_POSITIVE 290 218 175 137 73 NC_002967:TDE1253|3end_hypothetical_protein_1288473..1288623_POSITIVE 287 388 343 90 41 NC_002967:TDE1381|3end_V-type_ATP_synthase_subunit_E_1421288..1421438_NEGATIVE 280 165 127 81 40 NC_002967:TDE1867|3end_hypothetical_protein_1892351..1892501_NEGATIVE 275 178 134 100 49 NC_002967:TDE1827|3end_hydrolase,_carbon-nitrogen_family_1859943..1860093_NEGATIVE 272 368 324 68 20 NC_002967:TDE2236|3end_methylaspartate_mutase,_E_subunit_2274660..2274810_NEGATIVE 271 184 148 71 30 NC_002967:TDE1914|3end_Holliday_junction_DNA_helicase_RuvB_1930889..1931039_NEGATIVE 269 217 175 139 97 NC_002967:TDE0690|3end_tRNA_(5-methylaminomethyl-2-thiouridylate)-methyltransferase_728078..728228_POSITIVE 268 216 171 113 51 NC_002967:TDE0852|3end_50S_ribosomal_protein_L13_875527..875677_POSITIVE 266 368 340 75 42 NC_002967:TDE2347|3end_30S_ribosomal_protein_S2_2383503..2383653_NEGATIVE 266 178 131 133 85 NC_002967:TDE0701|3end_hypothetical_protein_736519..736669_NEGATIVE 265 192 151 78 40 NC_002967:TDE2178|3end_hypothetical_protein_2206683..2206833_NEGATIVE 263 128 81 73 24 NC_002967:TDE2773|3end_hypothetical_protein_2825823..2825973_NEGATIVE 263 249 202 69 26 NC_002967:TDE1719|3end_transcriptional_regulator,_MarR_family_1774546..1774696_POSITIVE