Origin IGS: ttataataaatacattacaaaatagtcgaggatttaactttttttaaatattacatttatgtatgacattattttgaacattaatacgaaaataaagactataactcagctgaccaaaggtgatattgaatatagtacaacaagactggagattagca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgctaatctccagtcttgttgtactatattcaatatcacctttggtcagctgagttatagtctttattttcgtattaatgttcaaaataatgtcatacataaatgtaatatttaaaaaaagttaaatcctcgactattttgtaatgtatttattataa Mask Tandem Repeat Region ================================================ ttataataaatacattacaaaatagtcgaggatttaactttttttaaatattacatttatgtatgacattattttgaacattaatacgaaaataaagactataactcagctgaccaaaggtgatattgaatatagtacaacaagactggagattagca Find is-nt database================================================ Query_seq: TDE0298:TDE0299|TDE0298:TDE0299:hypothetical protein:mutator mutT protein:->->:330062..330219 158 ttataataaatacattacaaaatagtcgaggatttaactttttttaaatattacatttatgtatgacattattttgaacattaatacgaaaataaagactataactcagctgaccaaaggtgatattgaatatagtacaacaagactggagattagca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE0298:TDE0299|TDE0298:TDE0299:hypothetical protein:mutator mutT protein:->->:330062..330219 158 ttataataaatacattacaaaatagtcgaggatttaactttttttaaatattacatttatgtatgacattattttgaacattaatacgaaaataaagactataactcagctgaccaaaggtgatattgaatatagtacaacaagactggagattagca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE0298:TDE0299|TDE0298:TDE0299:hypothetical protein:mutator mutT protein:->->:330062..330219 158 ttataataaatacattacaaaatagtcgaggatttaactttttttaaatattacatttatgtatgacattattttgaacattaatacgaaaataaagactataactcagctgaccaaaggtgatattgaatatagtacaacaagactggagattagca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttataataaatacattacaaaatagtcgaggatttaactttttttaaatattacatttatgtatgacattattttgaacattaatacgaaaataaagactataactcagctgaccaaaggtgatattgaatatagtacaacaagactggagattagca Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 50 End: 157 ................................................. M T F M Y D I I L N I N T K I K T I T Q L T K G D I E Y S T T R L E I S . ttataataaatacattacaaaatagtcgaggatttaactttttttaaatattacatttatgtatgacattattttgaacattaatacgaaaataaagactataactcagctgaccaaaggtgatattgaatatagtacaacaagactggagattagca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttataataaatacattacaaaatagtcgaggatttaactttttttaaatattacatttatgtatgacattattttgaacattaatacgaaaataaagactataactcagctgaccaaaggtgatattgaatatagtacaacaagactggagattagca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TDE0298:TDE0299|TDE0298:TDE0299:hypothetical protein:mutator mutT protein:->->:330062..330219 158 ttataataaatacattacaaaatagtcgaggatttaactttttttaaatattacatttatgtatgacattattttgaacattaatacgaaaataaagactataactcagctgaccaaaggtgatattgaatatagtacaacaagactggagattagca Intra-Species Hit: Count: 1 Min: 1 Max: 158 Len: 158 Subject: NC_002967_TDE0298_TDE0299|hypothetical protein:mutator mutT protein|POSITIVE:POSITIVE|[330062,330219]|158 HSP 1 e-value: 3.0E-72 bit: 272.0 Len: 158 Query Start:1 Query End:158 Subject Strand: POSITIVE Subject Start: 1 Subject End: 158 ttataataaatacattacaaaatagtcgaggatttaacnnnnnnnaaatattacatttatgtatgacattattttgaacattaatacgaaaataaagactataactcagctgaccaaaggtgatattgaatatagtacaacaagactggagattagca ttataataaatacattacaaaatagtcgaggatttaactttttttaaatattacatttatgtatgacattattttgaacattaatacgaaaataaagactataactcagctgaccaaaggtgatattgaatatagtacaacaagactggagattagca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAUAAUAAAUACAUUACAAAAUAGUCGAGGAUUUAACUUUUUUUAAAUAUUACAUUUAUGUAUGACAUUAUUUUGAACAUUAAUACGAAAAUAAAGACUAUAACUCAGCUGACCAAAGGUGAUAUUGAAUAUAGUACAACAAGACUGGAGAUUAGCA .....................((((((....((((((......)))))).....((((.(((((...................))))).))))...))))))......((((((((...((..(((((....)))))..)).....)))...))))). (-17.01) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGCUAAUCUCCAGUCUUGUUGUACUAUAUUCAAUAUCACCUUUGGUCAGCUGAGUUAUAGUCUUUAUUUUCGUAUUAAUGUUCAAAAUAAUGUCAUACAUAAAUGUAAUAUUUAAAAAAAGUUAAAUCCUCGACUAUUUUGUAAUGUAUUUAUUAUAA .(((.(((....((..(((((.........)))))..))....))).))).(((.......((((.(((..((((((((((.((......))....)))).....))))))..))).)))).......))).......((((((((....)))))))) (-17.04) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 284 139 92 193 145 NC_002967:TDE2340|5end_FMN-binding_domain_protein_2377855..2378085_NEGATIVE 234 140 96 219 173 NC_002967:TDE2329|5end_hypothetical_protein_2366493..2366723_NEGATIVE 224 139 94 85 31 NC_002967:TDE2045|5end_hypothetical_protein_2074933..2075163_NEGATIVE 214 150 108 220 174 NC_002967:TDE1354|5end_hypothetical_protein_1394886..1395116_POSITIVE 208 147 101 67 15 NC_002967:TDE1324|5end_hypothetical_protein_1361610..1361840_NEGATIVE 205 145 116 60 27 NC_002967:TDE2011|5end_hypothetical_protein_2031930..2032160_POSITIVE 201 146 109 178 143 NC_002967:TDE2507|5end_hypothetical_protein_2531735..2531965_NEGATIVE 194 150 119 168 138 NC_002967:TDE0328|5end_CRISPR-associated_protein_Cas1_365065..365295_POSITIVE 193 124 96 176 143 NC_002967:TDE2347|5end_30S_ribosomal_protein_S2_2384378..2384608_NEGATIVE 193 132 106 149 113 NC_002967:TDE0991|5end_ribosomal_protein_L28_1018065..1018295_NEGATIVE 193 149 111 183 146 NC_002967:TDE0483|5end_hypothetical_protein_538805..539035_POSITIVE 192 124 83 70 28 NC_002967:TDE1110|5end_lipoprotein,_putative_1144272..1144502_POSITIVE 188 139 99 90 55 NC_002967:TDE2643|5end_oxidoreductase,_FAD-dependent_2682185..2682415_POSITIVE 187 150 101 164 105 NC_002967:TDE1704|5end_hypothetical_protein_1760588..1760818_POSITIVE 186 128 82 95 49 NC_002967:TDE1137|5end_hypothetical_protein_1166749..1166979_NEGATIVE 186 123 83 63 28 NC_002967:TDE0415|5end_lipoprotein,_putative_467667..467897_POSITIVE 185 149 109 173 124 NC_002967:TDE1738|5end_M23/M37_peptidase_domain_protein_1790536..1790766_POSITIVE 185 146 108 75 47 NC_002967:TDE1018|5end_hypothetical_protein_1049227..1049457_POSITIVE 182 122 84 113 71 NC_002967:TDE0998|5end_hypothetical_protein_1025202..1025432_POSITIVE 180 143 105 175 126 NC_002967:TDE2304|5end_hypothetical_protein_2343440..2343670_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 208 147 101 57 5 NC_002967:TDE1325|3end_hypothetical_protein_1361620..1361770_NEGATIVE 192 124 83 47 5 NC_002967:TDE1109|3end_decarboxylase,_pyridoxal-dependent_family_1144329..1144479_POSITIVE 189 150 105 60 14 NC_002967:TDE2308|3end_hypothetical_protein_2346313..2346463_POSITIVE 187 150 101 134 75 NC_002967:TDE1703|3end_hypothetical_protein_1760638..1760788_POSITIVE 185 149 108 67 34 NC_002967:TDE1939|3end_hypothetical_protein_1955435..1955585_POSITIVE 178 150 108 128 82 NC_002967:TDE1943|3end_30S_ribosomal_protein_S4_1959436..1959586_NEGATIVE 174 115 86 32 6 NC_002967:TDE1876|3end_glycosyl_transferase,_WecB/TagA/CpsF_family_1896939..1897089_NEGATIVE 174 115 87 141 113 NC_002967:TDE1848|3end_hypothetical_protein_1875897..1876047_NEGATIVE 173 150 107 132 94 NC_002967:TDE1353|3end_rod_shape-determining_protein_RodA_1394931..1395081_POSITIVE 172 128 96 38 9 NC_002967:TDE1743|3end_chromate_transport_protein_1797940..1798090_NEGATIVE 172 147 125 144 118 NC_002967:TDE0283|3end_transporter,_putative_312121..312271_NEGATIVE 170 110 67 47 5 NC_002967:TDE2341|3end_membrane-associated_zinc_metalloprotease,_putative_2377866..2378016_NEGATIVE 170 150 109 118 73 NC_002967:TDE0466|3end_hypothetical_protein_518645..518795_NEGATIVE 165 140 94 149 99 NC_002967:TDE0701|3end_hypothetical_protein_736519..736669_NEGATIVE 165 124 83 53 17 NC_002967:TDE0420|3end_hypothetical_protein_473485..473635_POSITIVE 165 132 109 75 47 NC_002967:TDE0118|3end_hypothetical_protein_136574..136724_NEGATIVE 161 118 88 36 4 NC_002967:TDE0819|3end_ABC_transporter,_ATP-binding/permease_protein_841649..841799_NEGATIVE 161 131 100 126 92 NC_002967:TDE0493|3end_hypothetical_protein_548581..548731_NEGATIVE 160 148 108 144 99 NC_002967:TDE2303|3end_hypothetical_protein_2343527..2343677_POSITIVE 160 122 96 148 120 NC_002967:TDE2195|3end_RNA_pseudouridylate_synthase_family_protein_2231911..2232061_POSITIVE