Origin IGS: aagcactgctgctgtaatcttaattgatacaattaaatcttgcgcaaatcagacttgttttttctgttaatttgaatataattatgtatagaagaggtttct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agaaacctcttctatacataattatattcaaattaacagaaaaaacaagtctgatttgcgcaagatttaattgtatcaattaagattacagcagcagtgctt Mask Tandem Repeat Region ================================================ aagcactgctgctgtaatcttaattgatacaattaaatcttgcgcaaatcagacttgttttttctgttaatttgaatataattatgtatagaagaggtttct Find is-nt database================================================ Query_seq: TDE0248:TDE0249|TDE0248:TDE0249:hypothetical protein:flavoredoxin, putative:->->:282577..282678 102 aagcactgctgctgtaatcttaattgatacaattaaatcttgcgcaaatcagacttgttttttctgttaatttgaatataattatgtatagaagaggtttct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE0248:TDE0249|TDE0248:TDE0249:hypothetical protein:flavoredoxin, putative:->->:282577..282678 102 aagcactgctgctgtaatcttaattgatacaattaaatcttgcgcaaatcagacttgttttttctgttaatttgaatataattatgtatagaagaggtttct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE0248:TDE0249|TDE0248:TDE0249:hypothetical protein:flavoredoxin, putative:->->:282577..282678 102 aagcactgctgctgtaatcttaattgatacaattaaatcttgcgcaaatcagacttgttttttctgttaatttgaatataattatgtatagaagaggtttct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aagcactgctgctgtaatcttaattgatacaattaaatcttgcgcaaatcagacttgttttttctgttaatttgaatataattatgtatagaagaggtttct Predict ORF larger than 30AA ================================================ aagcactgctgctgtaatcttaattgatacaattaaatcttgcgcaaatcagacttgttttttctgttaatttgaatataattatgtatagaagaggtttct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aagcactgctgctgtaatcttaattgatacaattaaatcttgcgcaaatcagacttgttttttctgttaatttgaatataattatgtatagaagaggtttct Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TDE0248:TDE0249|TDE0248:TDE0249:hypothetical protein:flavoredoxin, putative:->->:282577..282678 102 aagcactgctgctgtaatcttaattgatacaattaaatcttgcgcaaatcagacttgttttttctgttaatttgaatataattatgtatagaagaggtttct Intra-Species Hit: Count: 1 Min: 1 Max: 102 Len: 102 Subject: NC_002967_TDE0248_TDE0249|hypothetical protein:flavoredoxin, putative|POSITIVE:POSITIVE|[282577,282678]|102 HSP 1 e-value: 2.0E-51 bit: 202.0 Len: 102 Query Start:1 Query End:102 Subject Strand: POSITIVE Subject Start: 1 Subject End: 102 aagcactgctgctgtaatcttaattgatacaattaaatcttgcgcaaatcagacttgttttttctgttaatttgaatataattatgtatagaagaggtttct aagcactgctgctgtaatcttaattgatacaattaaatcttgcgcaaatcagacttgttttttctgttaatttgaatataattatgtatagaagaggtttct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGCACUGCUGCUGUAAUCUUAAUUGAUACAAUUAAAUCUUGCGCAAAUCAGACUUGUUUUUUCUGUUAAUUUGAAUAUAAUUAUGUAUAGAAGAGGUUUCU .....(((.(((.((((..(((((((...)))))))...)))))))...))).....(((((((((((((((.......)))))...))))))))))..... (-14.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAAACCUCUUCUAUACAUAAUUAUAUUCAAAUUAACAGAAAAAACAAGUCUGAUUUGCGCAAGAUUUAAUUGUAUCAAUUAAGAUUACAGCAGCAGUGCUU ((((.....)))).................................(((((((...(((......((((((((...))))))))......))).))).)))) (-10.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 221 100 60 141 98 NC_002967:TDE1998|5end_phosphohexose_mutase_family_protein_2015300..2015530_POSITIVE 210 65 37 75 44 NC_002967:TDE1337|5end_hypothetical_protein_1374806..1375036_NEGATIVE 208 20 2 182 162 NC_002967:TDE2069|5end_endoribonuclease_L-PSP,_putative_2096632..2096862_POSITIVE 207 73 40 99 58 NC_002967:TDE0748|5end_iron_compound_ABC_transporter,_periplasmic_iron_compound-binding_protein,_putative_784288..784518_NEGATIVE 206 21 1 76 47 NC_002967:TDE2313|5end_hypothetical_protein_2352010..2352240_NEGATIVE 205 46 2 229 175 NC_002967:TDE1517|5end_hypothetical_protein_1563816..1564046_POSITIVE 203 68 23 87 33 NC_002967:TDE1818|5end_hypothetical_protein_1853791..1854021_NEGATIVE 201 76 38 80 39 NC_002967:TDE2320|5end_RNA_polymerase_sigma-24_factor,_putative_2356951..2357181_NEGATIVE 200 22 3 214 197 NC_002967:TDE2115|5end_HAMP_domain_protein_2144760..2144990_NEGATIVE 200 45 3 51 3 NC_002967:TDE1501|5end_pyruvate_phosphate_dikinase_1548041..1548271_POSITIVE 200 44 3 67 21 NC_002967:TDE1320|5end_ComEC/Rec2-related_protein_1357764..1357994_POSITIVE 199 48 3 126 88 NC_002967:TDE1137|5end_hypothetical_protein_1166749..1166979_NEGATIVE 199 50 3 116 59 NC_002967:TDE0596|5end_hypothetical_protein_636010..636240_NEGATIVE 198 75 36 77 37 NC_002967:TDE2682|5end_hypothetical_protein_2723358..2723588_NEGATIVE 197 43 2 61 31 NC_002967:TDE0731|5end_hypothetical_protein_771347..771577_NEGATIVE 197 47 2 224 186 NC_002967:TDE0255|5end_ABC_transporter,_ATP-binding_protein_290742..290972_NEGATIVE 194 43 2 140 103 NC_002967:TDE0034|5end_hypothetical_protein_41450..41680_NEGATIVE 191 75 41 163 124 NC_002967:TDE2405|5end_hypothetical_protein_2432050..2432280_NEGATIVE 191 47 5 150 115 NC_002967:TDE1795|5end_funZ_protein,_putative_1834673..1834903_NEGATIVE 191 50 9 82 30 NC_002967:TDE0406|5end_hypothetical_protein_457230..457460_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 221 50 1 116 56 NC_002967:TDE2276|3end_hypothetical_protein_2311945..2312095_NEGATIVE 218 47 2 149 105 NC_002967:TDE2708|3end_hypothetical_protein_2755136..2755286_NEGATIVE 209 48 4 40 1 NC_002967:TDE1021|3end_lipoprotein,_putative_1052590..1052740_NEGATIVE 208 20 2 65 45 NC_002967:TDE2068|3end_antigen,_putative_2096595..2096745_POSITIVE 201 76 38 83 42 NC_002967:TDE2321|3end_hypothetical_protein_2356948..2357098_NEGATIVE 200 44 3 73 27 NC_002967:TDE1319|3end_hypothetical_protein_1357850..1358000_POSITIVE 199 50 3 107 50 NC_002967:TDE0597|3end_hypothetical_protein_636019..636169_NEGATIVE 198 75 36 68 28 NC_002967:TDE2683|3end_sigma_factor_WhiG_2723367..2723517_NEGATIVE 197 50 3 138 68 NC_002967:TDE0635|3end_hypothetical_protein_670578..670728_POSITIVE 195 26 1 81 58 NC_002967:TDE0320|3end_CBS_domain_protein_352480..352630_NEGATIVE 194 28 5 151 128 NC_002967:TDE0049|3end_hypothetical_protein_58199..58349_NEGATIVE 191 47 5 57 22 NC_002967:TDE1796|3end_hypothetical_protein_1834766..1834916_NEGATIVE 190 66 30 104 57 NC_002967:TDE1049|3end_elongation_factor_EF-2_1077454..1077604_POSITIVE 189 14 3 44 33 NC_002967:TDE1750|3end_hypothetical_protein_1803439..1803589_NEGATIVE 189 97 51 131 86 NC_002967:TDE1133|3end_hypothetical_protein_1164753..1164903_NEGATIVE 187 34 1 75 39 NC_002967:TDE0099|3end_adenine-specific_DNA_modification_methyltransferase_117014..117164_POSITIVE 186 25 3 87 60 NC_002967:TDE1694|3end_hypothetical_protein_1749223..1749373_NEGATIVE 184 75 37 102 65 NC_002967:TDE0780|3end_ribosomal_protein_S14_812629..812779_POSITIVE 183 68 30 148 105 NC_002967:TDE2582|3end_GGDEF_domain_protein_2616164..2616314_POSITIVE 181 17 1 139 123 NC_002967:TDE2543|3end_hypothetical_protein_2570753..2570903_NEGATIVE