Origin IGS: gtaatgaagaaacctataactaactctggttccgtatttaaaaaacttaacatattctaagcaatagccacgtccggaatgctccgcttcctttcggggttttttattacagccccttaaaaaatcctagacctttccgtttaaaagttgtatattagtaac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gttactaatatacaacttttaaacggaaaggtctaggattttttaaggggctgtaataaaaaaccccgaaaggaagcggagcattccggacgtggctattgcttagaatatgttaagttttttaaatacggaaccagagttagttataggtttcttcattac Mask Tandem Repeat Region ================================================ gtaatgaagaaacctataactaactctggttccgtatttaaaaaacttaacatattctaagcaatagccacgtccggaatgctccgcttcctttcggggttttttattacagccccttaaaaaatcctagacctttccgtttaaaagttgtatattagtaac Find is-nt database================================================ Query_seq: TDE0007:TDE0008|TDE0007:TDE0008:hypothetical protein:copper-translocating P-type ATPase:->->:10869..11030 162 gtaatgaagaaacctataactaactctggttccgtatttaaaaaacttaacatattctaagcaatagccacgtccggaatgctccgcttcctttcggggttttttattacagccccttaaaaaatcctagacctttccgtttaaaagttgtatattagtaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TDE0007:TDE0008|TDE0007:TDE0008:hypothetical protein:copper-translocating P-type ATPase:->->:10869..11030 162 gtaatgaagaaacctataactaactctggttccgtatttaaaaaacttaacatattctaagcaatagccacgtccggaatgctccgcttcctttcggggttttttattacagccccttaaaaaatcctagacctttccgtttaaaagttgtatattagtaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TDE0007:TDE0008|TDE0007:TDE0008:hypothetical protein:copper-translocating P-type ATPase:->->:10869..11030 162 gtaatgaagaaacctataactaactctggttccgtatttaaaaaacttaacatattctaagcaatagccacgtccggaatgctccgcttcctttcggggttttttattacagccccttaaaaaatcctagacctttccgtttaaaagttgtatattagtaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gtaatgaagaaacctataactaactctggttccgtatttaaaaaacttaacatattctaagcaatagccacgtccggaatgctccgcttcctttcggggttttttattacagccccttaaaaaatcctagacctttccgtttaaaagttgtatattagtaac Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 54 End: 155 ..................................................... M L S N S H V R N A P L P F G V F Y Y S P L K N P R P F R L K V V Y ....... gtaatgaagaaacctataactaactctggttccgtatttaaaaaacttaacatattctaagcaatagccacgtccggaatgctccgcttcctttcggggttttttattacagccccttaaaaaatcctagacctttccgtttaaaagttgtatattagtaac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gtaatgaagaaacctataactaactctggttccgtatttaaaaaacttaacatattctaagcaatagccacgtccggaatgctccgcttcctttcggggttttttattacagccccttaaaaaatcctagacctttccgtttaaaagttgtatattagtaac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TDE0007:TDE0008|TDE0007:TDE0008:hypothetical protein:copper-translocating P-type ATPase:->->:10869..11030 162 gtaatgaagaaacctataactaactctggttccgtatttaaaaaacttaacatattctaagcaatagccacgtccggaatgctccgcttcctttcggggttttttattacagccccttaaaaaatcctagacctttccgtttaaaagttgtatattagtaac Intra-Species Hit: Count: 1 Min: 1 Max: 162 Len: 162 Subject: NC_002967_TDE0007_TDE0008|hypothetical protein:copper-translocating P-type ATPase|POSITIVE:POSITIVE|[10869,11030]|162 HSP 1 e-value: 4.0E-87 bit: 321.0 Len: 162 Query Start:1 Query End:162 Subject Strand: POSITIVE Subject Start: 1 Subject End: 162 gtaatgaagaaacctataactaactctggttccgtatttaaaaaacttaacatattctaagcaatagccacgtccggaatgctccgcttcctttcggggttttttattacagccccttaaaaaatcctagacctttccgtttaaaagttgtatattagtaac gtaatgaagaaacctataactaactctggttccgtatttaaaaaacttaacatattctaagcaatagccacgtccggaatgctccgcttcctttcggggttttttattacagccccttaaaaaatcctagacctttccgtttaaaagttgtatattagtaac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUAAUGAAGAAACCUAUAACUAACUCUGGUUCCGUAUUUAAAAAACUUAACAUAUUCUAAGCAAUAGCCACGUCCGGAAUGCUCCGCUUCCUUUCGGGGUUUUUUAUUACAGCCCCUUAAAAAAUCCUAGACCUUUCCGUUUAAAAGUUGUAUAUUAGUAAC ((((((((((..............(((((...(((..(((.....((((........))))...)))..))).)))))..((((((........)))))))))))))))).................(((((......)))))................... (-22.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUACUAAUAUACAACUUUUAAACGGAAAGGUCUAGGAUUUUUUAAGGGGCUGUAAUAAAAAACCCCGAAAGGAAGCGGAGCAUUCCGGACGUGGCUAUUGCUUAGAAUAUGUUAAGUUUUUUAAAUACGGAACCAGAGUUAGUUAUAGGUUUCUUCAUUAC (..(((.(((.(((((((.......((((((((...))))))))..((..(((((.((((((((.((....))...(((......)))((((((.(((.....)))..))))))..))))))))..)))))..)).))))).))))).)))..)........ (-32.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 341 104 61 86 29 NC_002967:TDE0793|5end_hypothetical_protein_818910..819140_POSITIVE 340 114 73 202 149 NC_002967:TDE2607|5end_hypothetical_protein_2648586..2648816_POSITIVE 326 107 61 132 75 NC_002967:TDE2680|5end_hypothetical_protein_2721072..2721302_NEGATIVE 326 97 55 225 182 NC_002967:TDE2393|5end_hypothetical_protein_2422398..2422628_POSITIVE 319 122 83 163 123 NC_002967:TDE1383|5end_hypothetical_protein_1422301..1422531_POSITIVE 310 92 61 138 109 NC_002967:TDE0957|5end_glycerophosphoryl_diester_phosphodiesterase_family_protein_980252..980482_POSITIVE 302 100 60 78 49 NC_002967:TDE2690|5end_hypothetical_protein_2728898..2729128_NEGATIVE 300 100 55 84 32 NC_002967:TDE2102|5end_hypothetical_protein_2127347..2127577_NEGATIVE 299 97 55 69 35 NC_002967:TDE0693|5end_phosphomethylpyrimidine_kinase_729942..730172_POSITIVE 298 118 71 188 121 NC_002967:TDE2223|5end_hypothetical_protein_2264642..2264872_POSITIVE 298 159 113 95 49 NC_002967:TDE1058|5end_hypothetical_protein_1086366..1086596_NEGATIVE 298 159 113 216 170 NC_002967:TDE1057|5end_hypothetical_protein_1086245..1086475_NEGATIVE 296 98 65 213 171 NC_002967:TDE1027|5end_ribonuclease_III_1057562..1057792_POSITIVE 296 115 71 121 69 NC_002967:TDE0674|5end_hypothetical_protein_713161..713391_POSITIVE 295 128 85 229 191 NC_002967:TDE2436|5end_hypothetical_protein_2466602..2466832_POSITIVE 294 97 57 218 177 NC_002967:TDE1314|5end_penicillin-binding_protein_1351174..1351404_POSITIVE 289 140 92 55 4 NC_002967:TDE1707|5end_hypothetical_protein_1763191..1763421_NEGATIVE 288 116 73 103 54 NC_002967:TDE2392|5end_hypothetical_protein_2422350..2422580_NEGATIVE 286 115 71 160 107 NC_002967:TDE1310|5end_modulator_of_DNA_gyrase_family_protein_1345962..1346192_POSITIVE 285 117 82 142 102 NC_002967:TDE0833|5end_lipoprotein,_putative_852992..853222_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 341 104 61 102 45 NC_002967:TDE0792|3end_ribosomal_protein_L17_819006..819156_POSITIVE 340 114 73 87 34 NC_002967:TDE2606|3end_urocanate_hydratase_2648551..2648701_POSITIVE 326 107 61 135 78 NC_002967:TDE2681|3end_hypothetical_protein_2721069..2721219_NEGATIVE 322 120 71 116 59 NC_002967:TDE0109|3end_phenylalanyl-tRNA_synthetase_alpha_subunit_128833..128983_NEGATIVE 321 105 69 67 19 NC_002967:TDE2614|3end_ApbE_family_protein_2656018..2656168_NEGATIVE 313 100 61 144 100 NC_002967:TDE0833|3end_lipoprotein,_putative_854097..854247_POSITIVE 310 92 61 136 107 NC_002967:TDE0956|3end_hypothetical_protein_980330..980480_POSITIVE 302 100 59 143 105 NC_002967:TDE2527|3end_hypothetical_protein_2555449..2555599_NEGATIVE 296 120 76 82 19 NC_002967:TDE1026|3end_acyl_carrier_protein_1057618..1057768_POSITIVE 295 108 66 85 38 NC_002967:TDE2558|3end_ABC_transporter,_ATP-binding/permease_protein_2583729..2583879_NEGATIVE 294 103 64 128 65 NC_002967:TDE2542|3end_antigen,_putative_2569441..2569591_NEGATIVE 289 140 92 58 7 NC_002967:TDE1708|3end_apolipoprotein_N-acyltransferase_1763188..1763338_NEGATIVE 285 117 82 145 105 NC_002967:TDE0832|3end_hypothetical_protein_853075..853225_POSITIVE 282 100 51 145 70 NC_002967:TDE0002|3end_DNA_gyrase,_B_subunit_3540..3690_POSITIVE 280 100 66 55 13 NC_002967:TDE2543|3end_hypothetical_protein_2570753..2570903_NEGATIVE 280 119 76 119 60 NC_002967:TDE0569|3end_hypothetical_protein_600162..600312_POSITIVE 277 114 71 62 16 NC_002967:TDE2028|3end_OmpA_family_protein_2057019..2057169_POSITIVE 277 119 74 125 67 NC_002967:TDE0040|3end_AMP-binding_protein_45776..45926_NEGATIVE 276 104 68 142 104 NC_002967:TDE1309|3end_modulator_of_DNA_gyrase_family_protein_1346042..1346192_POSITIVE 274 110 65 147 97 NC_002967:TDE2222|3end_hypothetical_protein_2264702..2264852_POSITIVE