Origin IGS: ctttgcggccatcggtctcctgatcgagaacctctataacctgcaatctcgaaaacacgaaccgaaatcgttccgattgtggcacgtgttttctgagaaaatggcgcgagatttttttcgttgtggcgcgtaaaatttttgcttcccgaaccaaaacaaaaaaattctcgcgccacctttttcagtacacgtcactgcggaaaaaaacttttccggccggaacgatcatttctttttatagcctgctgtattttgtgcttttgggattactcagactatgatatttttgctcctgattggatacatcctgattttagggagcaccggagattttccttatttcgattcattgtaaataaagatgctgttgaaaaatttttctctccttcctcgcaacctctttgtacacaactgaatcgaggctctgtcttgggtgttgataactcggtcgcgccttggtcggctttcgtataagtgtctggaatgcagtggaatagcgttgtttccttcgctgtttatatctcaaggttataaccatctgaaaacaatatagatacgattcatctgaattatactacactttttgtcgattgtttttttactcttttgtttgaacctttgctatccccaaaagagcaatgaacgactgctctcaaaacgatatttttttag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctaaaaaaatatcgttttgagagcagtcgttcattgctcttttggggatagcaaaggttcaaacaaaagagtaaaaaaacaatcgacaaaaagtgtagtataattcagatgaatcgtatctatattgttttcagatggttataaccttgagatataaacagcgaaggaaacaacgctattccactgcattccagacacttatacgaaagccgaccaaggcgcgaccgagttatcaacacccaagacagagcctcgattcagttgtgtacaaagaggttgcgaggaaggagagaaaaatttttcaacagcatctttatttacaatgaatcgaaataaggaaaatctccggtgctccctaaaatcaggatgtatccaatcaggagcaaaaatatcatagtctgagtaatcccaaaagcacaaaatacagcaggctataaaaagaaatgatcgttccggccggaaaagtttttttccgcagtgacgtgtactgaaaaaggtggcgcgagaatttttttgttttggttcgggaagcaaaaattttacgcgccacaacgaaaaaaatctcgcgccattttctcagaaaacacgtgccacaatcggaacgatttcggttcgtgttttcgagattgcaggttatagaggttctcgatcaggagaccgatggccgcaaag Mask Tandem Repeat Region ================================================ ctttgcggccatcggtctcctgatcgagaacctctataacctgcaatctcgaaaacacgaaccgaaatcgttccgattgtggcacgtgttttctgagaaaatggcgcgagatttttttcgttgtggcgcgtaaaatttttgcttcccgaaccaaaacaaaaaaattctcgcgccacctttttcagtacacgtcactgcggaaaaaaacttttccggccggaacgatcatttctttttatagcctgctgtattttgtgcttttgggattactcagactatgatatttttgctcctgattggatacatcctgattttagggagcaccggagattttccttatttcgattcattgtaaataaagatgctgttgaaaaatttttctctccttcctcgcaacctctttgtacacaactgaatcgaggctctgtcttgggtgttgataactcggtcgcgccttggtcggctttcgtataagtgtctggaatgcagtggaatagcgttgtttccttcgctgtttatatctcaaggttataaccatctgaaaacaatatagatacgattcatctgaattatactacactttttgtcgattgtttttttactcttttgtttgaacctttgctatccccaaaagagcaatgaacgactgctctcaaaacgatatttttttag Find is-nt database================================================ Query_seq: PG2227:PG0001|PG2227:PG0001:hypothetical protein:chromosomal replication initiation protein:->->:2342805..2343476 672 ctttgcggccatcggtctcctgatcgagaacctctataacctgcaatctcgaaaacacgaaccgaaatcgttccgattgtggcacgtgttttctgagaaaatggcgcgagatttttttcgttgtggcgcgtaaaatttttgcttcccgaaccaaaacaaaaaaattctcgcgccacctttttcagtacacgtcactgcggaaaaaaacttttccggccggaacgatcatttctttttatagcctgctgtattttgtgcttttgggattactcagactatgatatttttgctcctgattggatacatcctgattttagggagcaccggagattttccttatttcgattcattgtaaataaagatgctgttgaaaaatttttctctccttcctcgcaacctctttgtacacaactgaatcgaggctctgtcttgggtgttgataactcggtcgcgccttggtcggctttcgtataagtgtctggaatgcagtggaatagcgttgtttccttcgctgtttatatctcaaggttataaccatctgaaaacaatatagatacgattcatctgaattatactacactttttgtcgattgtttttttactcttttgtttgaacctttgctatccccaaaagagcaatgaacgactgctctcaaaacgatatttttttag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG2227:PG0001|PG2227:PG0001:hypothetical protein:chromosomal replication initiation protein:->->:2342805..2343476 672 ctttgcggccatcggtctcctgatcgagaacctctataacctgcaatctcgaaaacacgaaccgaaatcgttccgattgtggcacgtgttttctgagaaaatggcgcgagatttttttcgttgtggcgcgtaaaatttttgcttcccgaaccaaaacaaaaaaattctcgcgccacctttttcagtacacgtcactgcggaaaaaaacttttccggccggaacgatcatttctttttatagcctgctgtattttgtgcttttgggattactcagactatgatatttttgctcctgattggatacatcctgattttagggagcaccggagattttccttatttcgattcattgtaaataaagatgctgttgaaaaatttttctctccttcctcgcaacctctttgtacacaactgaatcgaggctctgtcttgggtgttgataactcggtcgcgccttggtcggctttcgtataagtgtctggaatgcagtggaatagcgttgtttccttcgctgtttatatctcaaggttataaccatctgaaaacaatatagatacgattcatctgaattatactacactttttgtcgattgtttttttactcttttgtttgaacctttgctatccccaaaagagcaatgaacgactgctctcaaaacgatatttttttag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG2227:PG0001|PG2227:PG0001:hypothetical protein:chromosomal replication initiation protein:->->:2342805..2343476 672 ctttgcggccatcggtctcctgatcgagaacctctataacctgcaatctcgaaaacacgaaccgaaatcgttccgattgtggcacgtgttttctgagaaaatggcgcgagatttttttcgttgtggcgcgtaaaatttttgcttcccgaaccaaaacaaaaaaattctcgcgccacctttttcagtacacgtcactgcggaaaaaaacttttccggccggaacgatcatttctttttatagcctgctgtattttgtgcttttgggattactcagactatgatatttttgctcctgattggatacatcctgattttagggagcaccggagattttccttatttcgattcattgtaaataaagatgctgttgaaaaatttttctctccttcctcgcaacctctttgtacacaactgaatcgaggctctgtcttgggtgttgataactcggtcgcgccttggtcggctttcgtataagtgtctggaatgcagtggaatagcgttgtttccttcgctgtttatatctcaaggttataaccatctgaaaacaatatagatacgattcatctgaattatactacactttttgtcgattgtttttttactcttttgtttgaacctttgctatccccaaaagagcaatgaacgactgctctcaaaacgatatttttttag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 4 Min: 32 Max: 182 Len: 151 Subject: UniRef90_Q7MU89 Cluster: Hypothetical protein; n=1; Porphyromonas gingivalis|Rep: Hypothetical protein - Porphyromonas gingivalis (Bacteroides gingivalis) HSP 1 e-value: 2.0E-11 bit: 72.0 Len: 129 Query Start:54 Query End:182 Subject Strand: null Subject Start: 12 Subject End: 54 ..................................................... N T N R N R S D C G T C F L R K W R E I F F V V A R K I F A S R T K T K K F S R H L F .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ..................................................... N T N Q K S S D C G S C F L K K W R E I F F V L A C K F F N C R A K T K K F S R H V F .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q7MTB1 Cluster: Hypothetical protein; n=1; Porphyromonas gingivalis|Rep: Hypothetical protein - Porphyromonas gingivalis (Bacteroides gingivalis) HSP 1 e-value: 2.0E-9 bit: 65.1 Len: 129 Query Start:53 Query End:181 Subject Strand: null Subject Start: 4 Subject End: 46 .................................................... K R W R E N F F V L V R E A K I L R A T T K K I S R H F L R K H V P Q S E R F R F V F ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... .................................................... K T W R E N F F V L V R E V K N L R A T A K K I S R H F F R K Y E P Q P G V F W F V F ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q7MXK3 Cluster: Pyridine nucleotide-disulphide oxidoreductase family protein; n=1; Porphyromonas gingivalis|Rep: Pyridine nucleotide-disulphide oxidoreductase family protein - Porphyromonas gingivalis (Bacteroides gingivalis) HSP 1 e-value: 9.0E-7 bit: 56.2 Len: 123 Query Start:32 Query End:154 Subject Strand: null Subject Start: 1 Subject End: 41 ............................... L V R E A K I L R A T T K K I S R H F L R K H V P Q S E R F R F V F S R L Q V I E ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ............................... M V R E A K N L R A R T K K I S L H I C R K H A P Q S E D F R C V F L R Q Q V I D ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q7MUH1 Cluster: Hypothetical protein; n=1; Porphyromonas gingivalis|Rep: Hypothetical protein - Porphyromonas gingivalis (Bacteroides gingivalis) HSP 1 e-value: 7.0E-6 bit: 53.1 Len: 132 Query Start:51 Query End:182 Subject Strand: null Subject Start: 20 Subject End: 63 .................................................. K K V A R E F F C F G S G S K N F T R H N E K N L A P F S Q K T R A T I G T I S V R V F .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... .................................................. Q N A V R K L F Y F G V G S K K F S R H N E K I L A P L F Q G I R A T I G A F P V R V F .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ctttgcggccatcggtctcctgatcgagaacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagtacacgtcactgcggaaaaaaacttttccggccggaacgatcatttctttttatagcctgctgtattttgtgcttttgggattactcagactatgatatttttgctcctgattggatacatcctgattttagggagcaccggagattttccttatttcgattcattgtaaataaagatgctgttgaaaaatttttctctccttcctcgcaacctctttgtacacaactgaatcgaggctctgtcttgggtgttgataactcggtcgcgccttggtcggctttcgtataagtgtctggaatgcagtggaatagcgttgtttccttcgctgtttatatctcaaggttataaccatctgaaaacaatatagatacgattcatctgaattatactacactttttgtcgattgtttttttactcttttgtttgaacctttgctatccccaaaagagcaatgaacgactgctctcaaaacgatatttttttag Predict ORF larger than 30AA ================================================ Protein_Len: 40 Strand: + Start: 11 End: 130 .......... M G L L I E N L Y N L Q S R K H E P K S F R L W H V F S E K M A R D F F R C G A .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 36 End: 278 ................................... M T C N L E N T N R N R S D C G T C F L R K W R E I F F V V A R K I F A S R T K T K K F S R H L F Q Y T S L R K K T F P .......................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 121 End: 471 ........................................................................................................................ M W R V K F L L P E P K Q K N S R A T F F S T R H C G K K L F R P E R S F L F I A C C I L C F W D Y S D Y D I F A P D W ......................................................................................................................................................................................................... Protein_Len: 45 Strand: + Start: 416 End: 550 ............................................................................................................................................................................................................................................................................................................................................................................................................................... M E A L S W V L I T R S R L G R L S Y K C L E C S G I A L F P S L F I S Q G Y N H L K T I .......................................................................................................................... Protein_Len: 30 Strand: + Start: 550 End: 639 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M D T I H L N Y T T L F V D C F F T L L F E P L L S P K E Q ................................. Protein_Len: 34 Strand: + Start: 569 End: 670 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M I L H F L S I V F L L F C L N L C Y P Q K S N E R L L S K R Y F F .. Protein_Len: 30 Strand: - Start: 573 End: 662 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ V V S K T S Q K K V R K N S G K S D G F S C H V V A R L V M .......... Protein_Len: 60 Strand: - Start: 350 End: 538 ............................................................................................................................................................................................................................................................................................................................................................. L H Q Q F I K R E K R A V E K Y V V S D L S Q R P H Q Y S P R A K T P K R I L T Q F A T S Y R Q K R R Q K Y R L T I V M ...................................................................................................................................... Protein_Len: 60 Strand: - Start: 340 End: 519 ................................................................................................................................................................................................................................................................................................................................................... K S E N Y I F I S N F F K E R R G R L R K T C L Q I S A R D Q T N I V R D R R P R S E Y L H R S H L P I A N N G E S N M ......................................................................................................................................................... Protein_Len: 60 Strand: - Start: 279 End: 485 ...................................................................................................................................................................................................................................................................................... G S K L S C R L N E K N R N M T F L S A T S F N K E G E E C G R Q V C S F R P E T K P T S L E T A G Q D A K T Y T D P M ........................................................................................................................................................................................... Protein_Len: 53 Strand: - Start: 37 End: 195 .................................... L R C D R F C S G F D N R N H C T N E S F I A R S K K R Q P A Y F K Q K G F W F L F I R A G G K E T C T M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 3 End: 185 .. P W R D G S R S G R Y G A I E F V R V S I T G I T A R T K Q S F P A L N K E N H R T F N K S G S G F C F F E R A V K K M ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 51 Strand: - Start: 2 End: 154 . K R G D T E Q D L V E I V Q L R S F V F R F R E S Q P V H K R L F H R S I K K T T A R L I K A E R V M ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ctttgcggccatcggtctcctgatcgagaacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagtacacgtcactgcggaaaaaaacttttccggccggaacgatcatttctttttatagcctgctgtattttgtgcttttgggattactcagactatgatatttttgctcctgattggatacatcctgattttagggagcaccggagattttccttatttcgattcattgtaaataaagatgctgttgaaaaatttttctctccttcctcgcaacctctttgtacacaactgaatcgaggctctgtcttgggtgttgataactcggtcgcgccttggtcggctttcgtataagtgtctggaatgcagtggaatagcgttgtttccttcgctgtttatatctcaaggttataaccatctgaaaacaatatagatacgattcatctgaattatactacactttttgtcgattgtttttttactcttttgtttgaacctttgctatccccaaaagagcaatgaacgactgctctcaaaacgatatttttttag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctttgcggccatcggtctcctgatcgagaacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagtacacgtcactgcggaaaaaaacttttccggccggaacgatcatttctttttatagcctgctgtattttgtgcttttgggattactcagactatgatatttttgctcctgattggatacatcctgattttagggagcaccggagattttccttatttcgattcattgtaaataaagatgctgttgaaaaatttttctctccttcctcgcaacctctttgtacacaactgaatcgaggctctgtcttgggtgttgataactcggtcgcgccttggtcggctttcgtataagtgtctggaatgcagtggaatagcgttgtttccttcgctgtttatatctcaaggttataaccatctgaaaacaatatagatacgattcatctgaattatactacactttttgtcgattgtttttttactcttttgtttgaacctttgctatccccaaaagagcaatgaacgactgctctcaaaacgatatttttttag Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 76 HP_score: -6.9 Tail_Score: -4.3739 Start: 56 End: 88 Full_Region: CTGCAATCTCGAAAA CACGAACCGAAATCG TTC CGATTGTGGCACGTG TTTTCTGAGAAAATG .......................................................CACGAACCGAAATCGTTCCGATTGTGGCACGTG........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 73 HP_score: -6.9 Tail_Score: -4.14392 Start: 56 End: 88 Full_Region: cattttctcagaaaa cacgtgccacaatcg gaa cgatttcggttcgtg ttttcgagattgcag .......................................................cacgtgccacaatcggaacgatttcggttcgtg........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Find igs database================================================ Query_seq: PG2227:PG0001|PG2227:PG0001:hypothetical protein:chromosomal replication initiation protein:->->:2342805..2343476 672 ctttgcggccatcggtctcctgatcgagaacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagtacacgtcactgcggaaaaaaacttttccggccggaacgatcatttctttttatagcctgctgtattttgtgcttttgggattactcagactatgatatttttgctcctgattggatacatcctgattttagggagcaccggagattttccttatttcgattcattgtaaataaagatgctgttgaaaaatttttctctccttcctcgcaacctctttgtacacaactgaatcgaggctctgtcttgggtgttgataactcggtcgcgccttggtcggctttcgtataagtgtctggaatgcagtggaatagcgttgtttccttcgctgtttatatctcaaggttataaccatctgaaaacaatatagatacgattcatctgaattatactacactttttgtcgattgtttttttactcttttgtttgaacctttgctatccccaaaagagcaatgaacgactgctctcaaaacgatatttttttag Intra-Species Hit: Count: 1 Min: 1 Max: 663 Len: 663 Subject: NC_002950_PG2227_PG0001|hypothetical protein:chromosomal replication initiation protein|POSITIVE:POSITIVE|[2342805,2343476]|672 HSP 1 e-value: 0.0 bit: 870.0 Len: 481 Query Start:183 Query End:663 Subject Strand: POSITIVE Subject Start: 183 Subject End: 663 ......................................................................................................................................................................................cagtacacgtcactgcggnnnnnnncttttccggccggaacgatcatttctttttatagcctgctgtattttgtgcttttgggattactcagactatgatatttttgctcctgattggatacatcctgattttagggagcaccggagattttccttatttcgattcattgtaaataaagatgctgttgaaaaatttttctctccttcctcgcaacctctttgtacacaactgaatcgaggctctgtcttgggtgttgataactcggtcgcgccttggtcggctttcgtataagtgtctggaatgcagtggaatagcgttgtttccttcgctgtttatatctcaaggttataaccatctgaaaacaatatagatacgattcatctgaattatactacactttttgtcgattgnnnnnnnactcttttgtttgaacctttgctatccccaaaagagcaatgaacgactgctctcaaaacgata......... ......................................................................................................................................................................................cagtacacgtcactgcggaaaaaaacttttccggccggaacgatcatttctttttatagcctgctgtattttgtgcttttgggattactcagactatgatatttttgctcctgattggatacatcctgattttagggagcaccggagattttccttatttcgattcattgtaaataaagatgctgttgaaaaatttttctctccttcctcgcaacctctttgtacacaactgaatcgaggctctgtcttgggtgttgataactcggtcgcgccttggtcggctttcgtataagtgtctggaatgcagtggaatagcgttgtttccttcgctgtttatatctcaaggttataaccatctgaaaacaatatagatacgattcatctgaattatactacactttttgtcgattgtttttttactcttttgtttgaacctttgctatccccaaaagagcaatgaacgactgctctcaaaacgata......... HSP 2 e-value: 3.0E-8 bit: 61.9 Len: 31 Query Start:1 Query End:31 Subject Strand: POSITIVE Subject Start: 1 Subject End: 31 ctttgcggccatcggtctcctgatcgagaac................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. ctttgcggccatcggtctcctgatcgagaac................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUUUGCGGCCAUCGGUCUCCUGAUCGAGAACCUCUAUAACCUGCAAUCUCGAAAACACGAACCGAAAUCGUUCCGAUUGUGGCACGUGUUUUCUGAGAAAAUGGCGCGAGAUUUUUUUCGUUGUGGCGCGUAAAAUUUUUGCUUCCCGAACCAAAACAAAAAAAUUCUCGCGCCACCUUUUUCAGUACACGUCACUGCGGAAAAAAACUUUUCCGGCCGGAACGAUCAUUUCUUUUUAUAGCCUGCUGUAUUUUGUGCUUUUGGGAUUACUCAGACUAUGAUAUUUUUGCUCCUGAUUGGAUACAUCCUGAUUUUAGGGAGCACCGGAGAUUUUCCUUAUUUCGAUUCAUUGUAAAUAAAGAUGCUGUUGAAAAAUUUUUCUCUCCUUCCUCGCAACCUCUUUGUACACAACUGAAUCGAGGCUCUGUCUUGGGUGUUGAUAACUCGGUCGCGCCUUGGUCGGCUUUCGUAUAAGUGUCUGGAAUGCAGUGGAAUAGCGUUGUUUCCUUCGCUGUUUAUAUCUCAAGGUUAUAACCAUCUGAAAACAAUAUAGAUACGAUUCAUCUGAAUUAUACUACACUUUUUGUCGAUUGUUUUUUUACUCUUUUGUUUGAACCUUUGCUAUCCCCAAAAGAGCAAUGAACGACUGCUCUCAAAACGAUA .....((((((..(((....((((((((......((((..(((.((((((((((.((((((..((((((((((((...(((..((((((...((((((((.((((((((((.((((((.(((.(((..((..((........))..))..))).))).)))))).))))))))))..)))))))).)))))))))..(((((((....)))))))..)))))))))...)))....(((((....))))).)))))).))))))))))...)))..))))...........(((((..(((....))).....))))).((((((.(((((...((.....((((((((((((..((((((((((.(..(((..............)))..))))...)))))))..)))).))))))))))....))))).)))))).....)))))))).))).))))))....((((..........(((..(((...((((((((..........)))))))).......((((((..(((.....(((((((((...((((.(((((....))))).............)))).)))))))))..........)))..)))))))))..))).....((((((.........))))))....)))).. (-189.54) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUCGUUUUGAGAGCAGUCGUUCAUUGCUCUUUUGGGGAUAGCAAAGGUUCAAACAAAAGAGUAAAAAAACAAUCGACAAAAAGUGUAGUAUAAUUCAGAUGAAUCGUAUCUAUAUUGUUUUCAGAUGGUUAUAACCUUGAGAUAUAAACAGCGAAGGAAACAACGCUAUUCCACUGCAUUCCAGACACUUAUACGAAAGCCGACCAAGGCGCGACCGAGUUAUCAACACCCAAGACAGAGCCUCGAUUCAGUUGUGUACAAAGAGGUUGCGAGGAAGGAGAGAAAAAUUUUUCAACAGCAUCUUUAUUUACAAUGAAUCGAAAUAAGGAAAAUCUCCGGUGCUCCCUAAAAUCAGGAUGUAUCCAAUCAGGAGCAAAAAUAUCAUAGUCUGAGUAAUCCCAAAAGCACAAAAUACAGCAGGCUAUAAAAAGAAAUGAUCGUUCCGGCCGGAAAAGUUUUUUUCCGCAGUGACGUGUACUGAAAAAGGUGGCGCGAGAAUUUUUUUGUUUUGGUUCGGGAAGCAAAAAUUUUACGCGCCACAACGAAAAAAAUCUCGCGCCAUUUUCUCAGAAAACACGUGCCACAAUCGGAACGAUUUCGGUUCGUGUUUUCGAGAUUGCAGGUUAUAGAGGUUCUCGAUCAGGAGACCGAUGGCCGCAAAG ....(((((((((((.(((.....(((((((((((.((((.......))))...)))))))))))..................((((.(.(((((((.......((((((.....((((((((((..(((....))))))))).))))..((((..(....)..))))......(((.....)))......))))))..(((......)))......)))))))).))))....))).(((((((((((((.(((((...(((((.((((....((((((........))))))..)))).)))))...))))))))))))).....(((.....)))..((((((.........(((....))).....))))))........((((((((.(((.................)))..))))))))..........(((((((((((.(((((((....)))))))..(((((((((.((((..((((((((((((((.((((((((((.((((.((..(((......)))..)).)))).)))))))))).)))))))))))))).))))...))))))..)))..)))))))))))..)))))..)))))))))))(((.((((((...(((.(((......)))))).)))))))))... (-202.77) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 737 150 102 51 3 NC_002950:PG2064|5end_hypothetical_protein_2160550..2160780_POSITIVE 703 180 136 117 73 NC_002950:PG0174|5end_pyridine_nucleotide-disulphide_oxidoreductase_family_protein_197628..197858_NEGATIVE 673 150 101 65 16 NC_002950:PG1799|5end_hypothetical_protein_1894499..1894729_POSITIVE 637 130 81 67 18 NC_002950:PG1980|5end_hypothetical_protein_2067930..2068160_NEGATIVE 538 239 194 101 55 NC_002950:PG0722|5end_hypothetical_protein_774891..775121_NEGATIVE 430 470 421 87 47 NC_002950:PG1494|5end_hypothetical_protein_1569841..1570071_POSITIVE 409 149 102 108 61 NC_002950:PG0007|5end_hypothetical_protein_6913..7143_NEGATIVE 406 128 85 138 94 NC_002950:PG2063|5end_hypothetical_protein_2160596..2160826_NEGATIVE 405 490 448 70 21 NC_002950:PG0422|5end_hypothetical_protein_462302..462532_NEGATIVE 397 257 212 63 17 NC_002950:PG1118|5end_clpB_protein_1194674..1194904_NEGATIVE 396 130 87 160 115 NC_002950:PG0081|5end_hypothetical_protein_93805..94035_POSITIVE 396 468 425 43 1 NC_002950:PG0072|5end_UDP-3-O-[3-hydroxymyristoyl]_glucosamine_N-acyltransferase_87147..87377_NEGATIVE 392 498 452 213 167 NC_002950:PG0212|5end_precorrin-6Y_C5,15-methyltransferase,_decarboxylating_249065..249295_NEGATIVE 387 220 172 58 17 NC_002950:PG0802|5end_alpha_keto_acid_dehydrogenase_complex,_E3_component,_lipoamide_dehydrogenase_859722..859952_NEGATIVE 385 157 116 60 19 NC_002950:PG0313|5end_hypothetical_protein_347366..347596_POSITIVE 383 499 453 59 1 NC_002950:PG0509|5end_hypothetical_protein_549588..549818_NEGATIVE 380 468 421 200 140 NC_002950:PG2107|5end_thiamine_biosynthesis_protein_ThiH_2216658..2216888_NEGATIVE 373 484 445 63 11 NC_002950:PG1540|5end_S-adenosylmethionine:tRNA_ribosyltransferase-isomerase_1619277..1619507_POSITIVE 371 201 166 40 1 NC_002950:PG1655|5end_hypothetical_protein_1737095..1737325_POSITIVE 371 470 422 198 138 NC_002950:PG0201|5end_ribonuclease_P_238770..239000_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 472 195 151 108 61 NC_002950:PG0732|3end_hypothetical_protein_783018..783168_POSITIVE 412 479 431 82 38 NC_002950:PG0547|3end_hypothetical_protein_601989..602139_POSITIVE 405 469 421 100 48 NC_002950:PG0555|3end_DNA-binding_protein,_histone-like_family_611716..611866_NEGATIVE 387 220 172 53 12 NC_002950:PG0803|3end_glucosamine-6-phosphate_deaminase_859727..859877_NEGATIVE 381 220 171 122 65 NC_002950:PG0143|3end_hydrolase,_carbon-nitrogen_family_165747..165897_NEGATIVE 377 459 416 123 90 NC_002950:PG0605|3end_hypothetical_protein_661165..661315_NEGATIVE 376 487 443 115 69 NC_002950:PG0378|3end_elongation_factor_Ts_408808..408958_POSITIVE 369 454 412 142 87 NC_002950:PG0344|3end_purple_acid_phosphatase_378543..378693_NEGATIVE 360 498 453 114 74 NC_002950:PG0032|3end_beta-mannosidase,_putative_37627..37777_NEGATIVE 359 469 423 142 85 NC_002950:PG1225|3end_ABC_transporter,_ATP-binding_protein_1303336..1303486_POSITIVE 359 469 421 136 53 NC_002950:PG0443|3end_hemagglutinin-related_protein_483147..483297_POSITIVE 358 492 451 88 43 NC_002950:PG1226|3end_peptidyl-prolyl_cis-trans_isomerase,_cyclophilin-type_1304048..1304198_POSITIVE 357 185 143 60 18 NC_002950:PG0542|3end_hypothetical_protein_595377..595527_NEGATIVE 355 490 447 141 103 NC_002950:PG1389|3end_DNA-binding_protein,_histone-like_family_1470870..1471020_NEGATIVE 355 480 431 117 67 NC_002950:PG0671|3end_iron_compound_ABC_transporter,_permease_protein_723038..723188_POSITIVE 354 330 284 74 32 NC_002950:PG0792|3end_hypoxanthine_phosphoribosyltransferase_843178..843328_NEGATIVE 354 515 476 139 101 NC_002950:PG0191|3end_outer_membrane_protein,_putative_229613..229763_POSITIVE 353 470 425 91 42 NC_002950:PG1875|3end_hemolysin_1969647..1969797_NEGATIVE 353 470 423 103 63 NC_002950:PG0721|3end_NLP/P60_family_protein_774535..774685_POSITIVE 350 490 444 90 47 NC_002950:PG2032|3end_primosomal_protein_N'_2130868..2131018_NEGATIVE