Origin IGS: tcttctccctctctcgcaaaaattctttcatttatatttggtttatactgtaaactaatttatatttgcatcggaagcggtaagaaatgccttgcggagacattttcaaatggaatacgaaccactaacggatagcaaaaaaatggaagaaaaacagcttacaccccaagagagcgtacag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctgtacgctctcttggggtgtaagctgtttttcttccatttttttgctatccgttagtggttcgtattccatttgaaaatgtctccgcaaggcatttcttaccgcttccgatgcaaatataaattagtttacagtataaaccaaatataaatgaaagaatttttgcgagagagggagaaga Mask Tandem Repeat Region ================================================ tcttctccctctctcgcaaaaattctttcatttatatttggtttatactgtaaactaatttatatttgcatcggaagcggtaagaaatgccttgcggagacattttcaaatggaatacgaaccactaacggatagcaaaaaaatggaagaaaaacagcttacaccccaagagagcgtacag Find is-nt database================================================ Query_seq: PG2223:PG2224|PG2223:PG2224:glycosyl transferase, group 2 family protein:hypothetical protein:->->:2338822..2339002 181 tcttctccctctctcgcaaaaattctttcatttatatttggtttatactgtaaactaatttatatttgcatcggaagcggtaagaaatgccttgcggagacattttcaaatggaatacgaaccactaacggatagcaaaaaaatggaagaaaaacagcttacaccccaagagagcgtacag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG2223:PG2224|PG2223:PG2224:glycosyl transferase, group 2 family protein:hypothetical protein:->->:2338822..2339002 181 tcttctccctctctcgcaaaaattctttcatttatatttggtttatactgtaaactaatttatatttgcatcggaagcggtaagaaatgccttgcggagacattttcaaatggaatacgaaccactaacggatagcaaaaaaatggaagaaaaacagcttacaccccaagagagcgtacag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG2223:PG2224|PG2223:PG2224:glycosyl transferase, group 2 family protein:hypothetical protein:->->:2338822..2339002 181 tcttctccctctctcgcaaaaattctttcatttatatttggtttatactgtaaactaatttatatttgcatcggaagcggtaagaaatgccttgcggagacattttcaaatggaatacgaaccactaacggatagcaaaaaaatggaagaaaaacagcttacaccccaagagagcgtacag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tcttctccctctctcgcaaaaattctttcatttatatttggtttatactgtaaactaatttatatttgcatcggaagcggtaagaaatgccttgcggagacattttcaaatggaatacgaaccactaacggatagcaaaaaaatggaagaaaaacagcttacaccccaagagagcgtacag Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 22 End: 132 ..................... M L S F I F G L Y C K L I Y I C I G S G K K C L A E T F S N G I R T T N G ................................................. Protein_Len: 40 Strand: + Start: 62 End: 181 ............................................................. M F A S E A V R N A L R R H F Q M E Y E P L T D S K K M E E K Q L T P Q E S V Q tcttctccctctctcgcaaaaattctttcatttatatttggtttatactgtaaactaatttatatttgcatcggaagcggtaagaaatgccttgcggagacattttcaaatggaatacgaaccactaacggatagcaaaaaaatggaagaaaaacagcttacaccccaagagagcgtacag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tcttctccctctctcgcaaaaattctttcatttatatttggtttatactgtaaactaatttatatttgcatcggaagcggtaagaaatgccttgcggagacattttcaaatggaatacgaaccactaacggatagcaaaaaaatggaagaaaaacagcttacaccccaagagagcgtacag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG2223:PG2224|PG2223:PG2224:glycosyl transferase, group 2 family protein:hypothetical protein:->->:2338822..2339002 181 tcttctccctctctcgcaaaaattctttcatttatatttggtttatactgtaaactaatttatatttgcatcggaagcggtaagaaatgccttgcggagacattttcaaatggaatacgaaccactaacggatagcaaaaaaatggaagaaaaacagcttacaccccaagagagcgtacag Intra-Species Hit: Count: 1 Min: 1 Max: 181 Len: 181 Subject: NC_002950_PG2223_PG2224|glycosyl transferase, group 2 family protein:hypothetical protein|POSITIVE:POSITIVE|[2338822,2339002]|181 HSP 1 e-value: 7.0E-86 bit: 317.0 Len: 181 Query Start:1 Query End:181 Subject Strand: POSITIVE Subject Start: 1 Subject End: 181 tcttctccctctctcgcaaaaattctttcatttatatttggtttatactgtaaactaatttatatttgcatcggaagcggtaagaaatgccttgcggagacattttcaaatggaatacgaaccactaacggatagcnnnnnnntggaagaaaaacagcttacaccccaagagagcgtacag tcttctccctctctcgcaaaaattctttcatttatatttggtttatactgtaaactaatttatatttgcatcggaagcggtaagaaatgccttgcggagacattttcaaatggaatacgaaccactaacggatagcaaaaaaatggaagaaaaacagcttacaccccaagagagcgtacag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUUCUCCCUCUCUCGCAAAAAUUCUUUCAUUUAUAUUUGGUUUAUACUGUAAACUAAUUUAUAUUUGCAUCGGAAGCGGUAAGAAAUGCCUUGCGGAGACAUUUUCAAAUGGAAUACGAACCACUAACGGAUAGCAAAAAAAUGGAAGAAAAACAGCUUACACCCCAAGAGAGCGUACAG ........((((((.((.....(((((((((((....(((((((((...)))))))))......((((((((.(...(.(((((......))))).).............(((........)))....).))).))))).))))))))))).....))..........))))))....... (-34.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUGUACGCUCUCUUGGGGUGUAAGCUGUUUUUCUUCCAUUUUUUUGCUAUCCGUUAGUGGUUCGUAUUCCAUUUGAAAAUGUCUCCGCAAGGCAUUUCUUACCGCUUCCGAUGCAAAUAUAAAUUAGUUUACAGUAUAAACCAAAUAUAAAUGAAAGAAUUUUUGCGAGAGAGGGAGAAGA .....(.((((((((.(((.((.(((((....((........(((((.(((.(..((((((.............((((.(((((.....)))))))))..))))))..)))))))))........))...))))).)).)))......((((......))))...)))))))).)...... (-42.05) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 365 102 62 127 79 NC_002950:PG0713|5end_anthranilate_synthase_component_II_767749..767979_NEGATIVE 344 103 68 91 55 NC_002950:PG2174|5end_hypothetical_protein_2282578..2282808_NEGATIVE 322 130 90 55 10 NC_002950:PG1289|5end_GDP-fucose_synthetase_1367041..1367271_POSITIVE 316 98 54 182 136 NC_002950:PG0727|5end_hypothetical_protein_778595..778825_NEGATIVE 297 103 68 152 116 NC_002950:PG1440|5end_hypothetical_protein_1527267..1527497_NEGATIVE 294 118 72 189 132 NC_002950:PG0664|5end_oxidoreductase,_Gfo/Idh/MocA_family_710293..710523_POSITIVE 294 120 71 81 33 NC_002950:PG0357|5end_aspartate_carbamoyltransferase_catalytic_subunit_391440..391670_POSITIVE 288 102 67 112 73 NC_002950:PG0767|5end_4-alpha-glucanotransferase_820533..820763_NEGATIVE 288 135 92 85 20 NC_002950:PG0264|5end_glycosyl_transferase,_group_2_family_protein_296975..297205_POSITIVE 285 107 68 196 154 NC_002950:PG0227|5end_DNA_repair_protein_RadA_263978..264208_NEGATIVE 283 103 65 155 115 NC_002950:PG1639|5end_hypothetical_protein_1719919..1720149_NEGATIVE 283 107 65 135 84 NC_002950:PG0934|5end_hypothetical_protein_994318..994548_NEGATIVE 282 130 82 188 139 NC_002950:PG0081|5end_hypothetical_protein_93805..94035_POSITIVE 279 120 72 194 150 NC_002950:PG0851|5end_hypothetical_protein_912732..912962_NEGATIVE 279 99 66 96 62 NC_002950:PG0442|5end_hypothetical_protein_481882..482112_NEGATIVE 278 108 68 138 101 NC_002950:PG1944|5end_3-phosphoshikimate_1-carboxyvinyltransferase_2030416..2030646_POSITIVE 278 100 65 43 6 NC_002950:PG1641|5end_phosphotyrosine_protein_phosphatase_1721763..1721993_NEGATIVE 277 106 65 175 136 NC_002950:PG0217|5end_hypothetical_protein_252490..252720_POSITIVE 272 107 61 185 135 NC_002950:PG1621|5end_hypothetical_protein_1701431..1701661_NEGATIVE 271 108 63 96 40 NC_002950:PG0700|5end_hypothetical_protein_756595..756825_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 322 130 90 62 17 NC_002950:PG1288|3end_GDP-mannose_4,6-dehydratase_1367128..1367278_POSITIVE 316 98 54 136 90 NC_002950:PG0728|3end_hypothetical_protein_778641..778791_NEGATIVE 316 107 61 141 92 NC_002950:PG0504|3end_lipoyl_synthase_545162..545312_POSITIVE 301 100 62 119 78 NC_002950:PG2041|3end_hypothetical_protein_2139235..2139385_NEGATIVE 297 103 68 61 25 NC_002950:PG1441|3end_lysozyme-related_protein_1527358..1527508_NEGATIVE 292 103 69 37 8 NC_002950:PG1486|3end_conjugative_transposon_protein_TraA_1560357..1560507_NEGATIVE 287 104 61 103 62 NC_002950:PG1375|3end_hypothetical_protein_1456743..1456893_POSITIVE 285 107 68 150 108 NC_002950:PG0228|3end_DdaH_family_protein_264024..264174_NEGATIVE 283 103 70 35 1 NC_002950:PG2175|3end_hypothetical_protein_2282634..2282784_NEGATIVE 283 103 65 139 99 NC_002950:PG1640|3end_DNA-damage-inducible_protein_F_1719935..1720085_NEGATIVE 277 106 65 141 102 NC_002950:PG0216|3end_hypothetical_protein_252536..252686_POSITIVE 273 99 69 106 70 NC_002950:PG1859|3end_glycerate_kinase_family_protein_1957174..1957324_NEGATIVE 269 99 65 149 105 NC_002950:PG0935|3end_4-diphosphocytidyl-2C-methyl-D-erythritol_kinase_994297..994447_NEGATIVE 269 99 68 144 107 NC_002950:PG0932|3end_DNA_polymerase_III,_delta_prime_subunit,_putative_989833..989983_POSITIVE 268 102 65 106 54 NC_002950:PG0723|3end_hypothetical_protein_775355..775505_POSITIVE 265 108 70 101 63 NC_002950:PG1334|3end_band_7/Mec-2_family_protein_1415422..1415572_NEGATIVE 262 130 85 147 106 NC_002950:PG1840|3end_hypothetical_protein_1937295..1937445_POSITIVE 262 101 65 113 69 NC_002950:PG1321|3end_formate--tetrahydrofolate_ligase_1399656..1399806_NEGATIVE 262 120 71 75 29 NC_002950:PG0560|3end_hypothetical_protein_617748..617898_POSITIVE 259 178 145 144 99 NC_002950:PG1340|3end_L-lactate_permease_1420298..1420448_NEGATIVE