Origin IGS: gacacagaattattattccaatttgtgtcgcaaaagtagctttttcccctatatatacctataagtatggaggcatttgaggaaaaatttccacatagttttttccgaccttttgcccccatattccgcaggtctttatcgaaagaaagcttttacactgtttgccaactataagaggtagcaaatcagtactttagaagccattacgagcactcccatcctgtgcagagtcgagatcattgtttcttaggcaaaagtacactctgaccgacaacgtcaaaagatatagagccttagaaaccctttgattaggcttgatttccggcatagcaggagtggacgaagctatgccggtgcgaatgacacttgtgcccccttaccaatcggcacaccactcggcatacgcatactcgcatcccgatgccaacaaactcgaaccgccgaaaaaaggaatcgggcaccttcctcttcgacaaaaacacgaaccataaatggacaaaacatggttcgtgaatttcgagaaactcgagcctaaaaattattcttttggctcgcaaaaatttcacttctcaaaccaaaagcaaaattttcccgcgccacgtttttcagaaccacggatcacaatcttttggcaccacaaataaaactctcggagccatcggtctttttatctcagcaaggatggtaaaattcgaccgattacaacaatgccctaaacctttttcggcttagggcattgttggatagagtcatgtttttccgaggcagtcggaaggt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 accttccgactgcctcggaaaaacatgactctatccaacaatgccctaagccgaaaaaggtttagggcattgttgtaatcggtcgaattttaccatccttgctgagataaaaagaccgatggctccgagagttttatttgtggtgccaaaagattgtgatccgtggttctgaaaaacgtggcgcgggaaaattttgcttttggtttgagaagtgaaatttttgcgagccaaaagaataatttttaggctcgagtttctcgaaattcacgaaccatgttttgtccatttatggttcgtgtttttgtcgaagaggaaggtgcccgattccttttttcggcggttcgagtttgttggcatcgggatgcgagtatgcgtatgccgagtggtgtgccgattggtaagggggcacaagtgtcattcgcaccggcatagcttcgtccactcctgctatgccggaaatcaagcctaatcaaagggtttctaaggctctatatcttttgacgttgtcggtcagagtgtacttttgcctaagaaacaatgatctcgactctgcacaggatgggagtgctcgtaatggcttctaaagtactgatttgctacctcttatagttggcaaacagtgtaaaagctttctttcgataaagacctgcggaatatgggggcaaaaggtcggaaaaaactatgtggaaatttttcctcaaatgcctccatacttataggtatatataggggaaaaagctacttttgcgacacaaattggaataataattctgtgtc Mask Tandem Repeat Region ================================================ gacacagaattattattccaatttgtgtcgcaaaagtagctttttcccctatatatacctataagtatggaggcatttgaggaaaaatttccacatagttttttccgaccttttgcccccatattccgcaggtctttatcgaaagaaagcttttacactgtttgccaactataagaggtagcaaatcagtactttagaagccattacgagcactcccatcctgtgcagagtcgagatcattgtttcttaggcaaaagtacactctgaccgacaacgtcaaaagatatagagccttagaaaccctttgattaggcttgatttccggcatagcaggagtggacgaagctatgccggtgcgaatgacacttgtgcccccttaccaatcggcacaccactcggcatacgcatactcgcatcccgatgccaacaaactcgaaccgccgaaaaaaggaatcgggcaccttcctcttcgacaaaaacacgaaccataaatggacaaaacatggttcgtgaatttcgagaaactcgagcctaaaaattattcttttggctcgcaaaaatttcacttctcaaaccaaaagcaaaattttcccgcgccacgtttttcagaaccacggatcacaatcttttggcaccacaaataaaactctcggagccatcggtctttttatctcagcaaggatggtaaaattcgaccgattacaacaatgccctaaacctttttcggcttagggcattgttggatagagtcatgtttttccgaggcagtcggaaggt Find is-nt database================================================ Query_seq: PG2182:PG2185|PG2182:PG2185:Na(+)-translocating NADH-quinone reductase subunit A:transporter, putative:<-<-:2291246..2292022 777 gacacagaattattattccaatttgtgtcgcaaaagtagctttttcccctatatatacctataagtatggaggcatttgaggaaaaatttccacatagttttttccgaccttttgcccccatattccgcaggtctttatcgaaagaaagcttttacactgtttgccaactataagaggtagcaaatcagtactttagaagccattacgagcactcccatcctgtgcagagtcgagatcattgtttcttaggcaaaagtacactctgaccgacaacgtcaaaagatatagagccttagaaaccctttgattaggcttgatttccggcatagcaggagtggacgaagctatgccggtgcgaatgacacttgtgcccccttaccaatcggcacaccactcggcatacgcatactcgcatcccgatgccaacaaactcgaaccgccgaaaaaaggaatcgggcaccttcctcttcgacaaaaacacgaaccataaatggacaaaacatggttcgtgaatttcgagaaactcgagcctaaaaattattcttttggctcgcaaaaatttcacttctcaaaccaaaagcaaaattttcccgcgccacgtttttcagaaccacggatcacaatcttttggcaccacaaataaaactctcggagccatcggtctttttatctcagcaaggatggtaaaattcgaccgattacaacaatgccctaaacctttttcggcttagggcattgttggatagagtcatgtttttccgaggcagtcggaaggt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG2182:PG2185|PG2182:PG2185:Na(+)-translocating NADH-quinone reductase subunit A:transporter, putative:<-<-:2291246..2292022 777 gacacagaattattattccaatttgtgtcgcaaaagtagctttttcccctatatatacctataagtatggaggcatttgaggaaaaatttccacatagttttttccgaccttttgcccccatattccgcaggtctttatcgaaagaaagcttttacactgtttgccaactataagaggtagcaaatcagtactttagaagccattacgagcactcccatcctgtgcagagtcgagatcattgtttcttaggcaaaagtacactctgaccgacaacgtcaaaagatatagagccttagaaaccctttgattaggcttgatttccggcatagcaggagtggacgaagctatgccggtgcgaatgacacttgtgcccccttaccaatcggcacaccactcggcatacgcatactcgcatcccgatgccaacaaactcgaaccgccgaaaaaaggaatcgggcaccttcctcttcgacaaaaacacgaaccataaatggacaaaacatggttcgtgaatttcgagaaactcgagcctaaaaattattcttttggctcgcaaaaatttcacttctcaaaccaaaagcaaaattttcccgcgccacgtttttcagaaccacggatcacaatcttttggcaccacaaataaaactctcggagccatcggtctttttatctcagcaaggatggtaaaattcgaccgattacaacaatgccctaaacctttttcggcttagggcattgttggatagagtcatgtttttccgaggcagtcggaaggt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG2182:PG2185|PG2182:PG2185:Na(+)-translocating NADH-quinone reductase subunit A:transporter, putative:<-<-:2291246..2292022 777 gacacagaattattattccaatttgtgtcgcaaaagtagctttttcccctatatatacctataagtatggaggcatttgaggaaaaatttccacatagttttttccgaccttttgcccccatattccgcaggtctttatcgaaagaaagcttttacactgtttgccaactataagaggtagcaaatcagtactttagaagccattacgagcactcccatcctgtgcagagtcgagatcattgtttcttaggcaaaagtacactctgaccgacaacgtcaaaagatatagagccttagaaaccctttgattaggcttgatttccggcatagcaggagtggacgaagctatgccggtgcgaatgacacttgtgcccccttaccaatcggcacaccactcggcatacgcatactcgcatcccgatgccaacaaactcgaaccgccgaaaaaaggaatcgggcaccttcctcttcgacaaaaacacgaaccataaatggacaaaacatggttcgtgaatttcgagaaactcgagcctaaaaattattcttttggctcgcaaaaatttcacttctcaaaccaaaagcaaaattttcccgcgccacgtttttcagaaccacggatcacaatcttttggcaccacaaataaaactctcggagccatcggtctttttatctcagcaaggatggtaaaattcgaccgattacaacaatgccctaaacctttttcggcttagggcattgttggatagagtcatgtttttccgaggcagtcggaaggt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gacacagaattattattccaatttgtgtcgcaaaagtagctttttcccctatatatacctataagtatggaggcatttgaggaaaaatttccacatagttttttccgaccttttgcccccatattccgcaggtctttatcgaaagaaagcttttacactgtttgccaactataagaggtagcaaatcagtactttagaagccattacgagcactcccatcctgtgcagagtcgagatcattgtttcttaggcaaaagtacactctgaccgacaacgtcaaaagatatagagccttagaaaccctttgattaggcttgatttccggcatagcaggagtggacgaagctatgccggtgcgaatgacacttgtgcccccttaccaatcggcacaccactcggcatacgcatactcgcatcccgatgccaacaaactcgaaccgccgaaaaaaggaatcgggcaccttcctcttcgacaaaaacacgaaccataaatggacaaaacatggttcgtgaatttcgagaaactcgagcctaaaaattattcttttggctcgcaaaaatttcacttctcaaaccaaaagcaaaattttcccgcgccacgtttttcagaaccacggatcacaatcttttggcaccacaaataaaactctcggagccatcggtctttttatctcagcaaggatggtaaaattcgaccgattacaacaatgccctaaacctttttcggcttagggcattgttggatagagtcatgtttttccgaggcagtcggaaggt Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 55 End: 171 ...................................................... M P I S M E A F E E K F P H S F F R P F A P I F R R S L S K E S F Y T V C Q L .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 34 Strand: + Start: 77 End: 178 ............................................................................ M R K N F H I V F S D L L P P Y S A G L Y R K K A F T L F A N Y K R ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 58 Strand: + Start: 315 End: 488 .......................................................................................................................................................................................................................................................................................................................... M I S G I A G V D E A M P V R M T L V P P Y Q S A H H S A Y A Y S H P D A N K L E P P K K G I G H L P L R Q K H E P ................................................................................................................................................................................................................................................................................................. Protein_Len: 48 Strand: + Start: 367 End: 510 .............................................................................................................................................................................................................................................................................................................................................................................. M C P L T N R H T T R H T H T R I P M P T N S N R R K K E S G T F L F D K N T N H K W T K H G S ........................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 305 End: 532 ................................................................................................................................................................................................................................................................................................................ M I R L D F R H S R S G R S Y A G A N D T C A P L P I G T P L G I R I L A S R C Q Q T R T A E K R N R A P S S S T K T R ..................................................................................................................................................................................................................................................... Protein_Len: 50 Strand: + Start: 492 End: 641 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M D K T W F V N F E K L E P K N Y S F G S Q K F H F S N Q K Q N F P A P R F S E P R I T I F W H H K ........................................................................................................................................ Protein_Len: 42 Strand: + Start: 560 End: 685 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M S L L K P K A K F S R A T F F R T T D H N L L A P Q I K L S E P S V F L S Q Q G W ............................................................................................ Protein_Len: 60 Strand: + Start: 514 End: 729 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M S R N S S L K I I L L A R K N F T S Q T K S K I F P R H V F Q N H G S Q S F G T T N K T L G A I G L F I S A R M V K F ................................................ Protein_Len: 40 Strand: - Start: 380 End: 499 ........................................................................................................................................................................................................................................................................................................................................................................................... W D A C W E A Y A Y E C G S A L L S S G G F F P I P C R G R R C F C S G Y I S M ...................................................................................................................................................................................................................................................................................... Protein_Len: 52 Strand: - Start: 349 End: 504 ............................................................................................................................................................................................................................................................................................................................................................ A P A F S V Q A G K G I P V G S P M R M S A D R H W C V R V A S F L F R A G E E E V F V R V M F P C F M ................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 312 End: 566 ....................................................................................................................................................................................................................................................................................................................... C V V R C V C V R M G I G V F E F R R F F S D P V K R K S L F V F W L H V F C P E H I E L F E L R F I I R K A R L F K M ................................................................................................................................................................................................................... Protein_Len: 55 Strand: - Start: 197 End: 361 .................................................................................................................................................................................................... F G N R A S G D Q A S D L D N N R L C F Y V R V S L T L L Y L A K S V R Q N P K I E P M A P T S S A I G T R M ................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 41 Strand: - Start: 62 End: 184 ............................................................. L Y P P M Q P F I E V Y N K G V K Q G W I G C T K I S L F S K C Q K G V I L P L M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 43 Strand: - Start: 3 End: 131 .. V S N N N W N T D C F Y S K G R Y I G I L I S A N S S F N G C L K K R G K A G M N R M ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... gacacagaattattattccaatttgtgtcgcaaaagtagctttttcccctatatatacctataagtatggaggcatttgaggaaaaatttccacatagttttttccgaccttttgcccccatattccgcaggtctttatcgaaagaaagcttttacactgtttgccaactataagaggtagcaaatcagtactttagaagccattacgagcactcccatcctgtgcagagtcgagatcattgtttcttaggcaaaagtacactctgaccgacaacgtcaaaagatatagagccttagaaaccctttgattaggcttgatttccggcatagcaggagtggacgaagctatgccggtgcgaatgacacttgtgcccccttaccaatcggcacaccactcggcatacgcatactcgcatcccgatgccaacaaactcgaaccgccgaaaaaaggaatcgggcaccttcctcttcgacaaaaacacgaaccataaatggacaaaacatggttcgtgaatttcgagaaactcgagcctaaaaattattcttttggctcgcaaaaatttcacttctcaaaccaaaagcaaaattttcccgcgccacgtttttcagaaccacggatcacaatcttttggcaccacaaataaaactctcggagccatcggtctttttatctcagcaaggatggtaaaattcgaccgattacaacaatgccctaaacctttttcggcttagggcattgttggatagagtcatgtttttccgaggcagtcggaaggt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gacacagaattattattccaatttgtgtcgcaaaagtagctttttcccctatatatacctataagtatggaggcatttgaggaaaaatttccacatagttttttccgaccttttgcccccatattccgcaggtctttatcgaaagaaagcttttacactgtttgccaactataagaggtagcaaatcagtactttagaagccattacgagcactcccatcctgtgcagagtcgagatcattgtttcttaggcaaaagtacactctgaccgacaacgtcaaaagatatagagccttagaaaccctttgattaggcttgatttccggcatagcaggagtggacgaagctatgccggtgcgaatgacacttgtgcccccttaccaatcggcacaccactcggcatacgcatactcgcatcccgatgccaacaaactcgaaccgccgaaaaaaggaatcgggcaccttcctcttcgacaaaaacacgaaccataaatggacaaaacatggttcgtgaatttcgagaaactcgagcctaaaaattattcttttggctcgcaaaaatttcacttctcaaaccaaaagcaaaattttcccgcgccacgtttttcagaaccacggatcacaatcttttggcaccacaaataaaactctcggagccatcggtctttttatctcagcaaggatggtaaaattcgaccgattacaacaatgccctaaacctttttcggcttagggcattgttggatagagtcatgtttttccgaggcagtcggaaggt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 78 HP_score: -5.7 Tail_Score: -4.82901 Start: 480 End: 512 Full_Region: gagtttctcgaaatt cacgaaccatg ttttgtccatt tatggttcgtg tttttgtcgaagagg ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cacgaaccatgttttgtccatttatggttcgtg......................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 59 HP_score: -4.9 Tail_Score: -3.90048 Start: 694 End: 752 Full_Region: AGGATGGTAAAATTC GACCGATTACAACAATGCCCTAAACC TTTTTC GGCTTAGGGCATTGTTGGATAGAGTCA TGTTTTTCCGAGGCA .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GACCGATTACAACAATGCCCTAAACCTTTTTCGGCTTAGGGCATTGTTGGATAGAGTCA......................... TransTerm Strand: - Conf: 100 HP_score: -16.2 Tail_Score: -3.4252 Start: 706 End: 739 Full_Region: catgactctatccaa caatgccctaagcc gaaaaa ggtttagggcattg ttgtaatcggtcgaa .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................caatgccctaagccgaaaaaggtttagggcattg...................................... TransTerm Strand: - Conf: 100 HP_score: -12.1 Tail_Score: -3.8512 Start: 709 End: 736 Full_Region: gactctatccaacaa tgccctaagcc gaaaaa ggtttagggca ttgttgtaatcggtc ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgccctaagccgaaaaaggtttagggca......................................... Find igs database================================================ Query_seq: PG2182:PG2185|PG2182:PG2185:Na(+)-translocating NADH-quinone reductase subunit A:transporter, putative:<-<-:2291246..2292022 777 gacacagaattattattccaatttgtgtcgcaaaagtagctttttcccctatatatacctataagtatggaggcatttgaggaaaaatttccacatagttttttccgaccttttgcccccatattccgcaggtctttatcgaaagaaagcttttacactgtttgccaactataagaggtagcaaatcagtactttagaagccattacgagcactcccatcctgtgcagagtcgagatcattgtttcttaggcaaaagtacactctgaccgacaacgtcaaaagatatagagccttagaaaccctttgattaggcttgatttccggcatagcaggagtggacgaagctatgccggtgcgaatgacacttgtgcccccttaccaatcggcacaccactcggcatacgcatactcgcatcccgatgccaacaaactcgaaccgccgaaaaaaggaatcgggcaccttcctcttcgacaaaaacacgaaccataaatggacaaaacatggttcgtgaatttcgagaaactcgagcctaaaaattattcttttggctcgcaaaaatttcacttctcaaaccaaaagcaaaattttcccgcgccacgtttttcagaaccacggatcacaatcttttggcaccacaaataaaactctcggagccatcggtctttttatctcagcaaggatggtaaaattcgaccgattacaacaatgccctaaacctttttcggcttagggcattgttggatagagtcatgtttttccgaggcagtcggaaggt Intra-Species Hit: Count: 1 Min: 1 Max: 777 Len: 777 Subject: NC_002950_PG2182_PG2185|Na(+)-translocating NADH-quinone reductase subunit A:transporter, putative|NEGATIVE:NEGATIVE|[2291246,2292022]|777 HSP 1 e-value: 0.0 bit: 1540.0 Len: 777 Query Start:1 Query End:777 Subject Strand: POSITIVE Subject Start: 1 Subject End: 777 gacacagaattattattccaatttgtgtcgcaaaagtagctttttcccctatatatacctataagtatggaggcatttgaggaaaaatttccacatagttttttccgaccttttgcccccatattccgcaggtctttatcgaaagaaagcttttacactgtttgccaactataagaggtagcaaatcagtactttagaagccattacgagcactcccatcctgtgcagagtcgagatcattgtttcttaggcaaaagtacactctgaccgacaacgtcaaaagatatagagccttagaaaccctttgattaggcttgatttccggcatagcaggagtggacgaagctatgccggtgcgaatgacacttgtgcccccttaccaatcggcacaccactcggcatacgcatactcgcatcccgatgccaacaaactcgaaccgccgaaaaaaggaatcgggcaccttcctcttcgacaaaaacacgaaccataaatggacaaaacatggttcgtgaatttcgagaaactcgagcctaaaaattattcttttggctcgcaaaaatttcacttctcaaaccaaaagcaaaattttcccgcgccacgtttttcagaaccacggatcacaatcttttggcaccacaaataaaactctcggagccatcggtctttttatctcagcaaggatggtaaaattcgaccgattacaacaatgccctaaacctttttcggcttagggcattgttggatagagtcatgtttttccgaggcagtcggaaggt gacacagaattattattccaatttgtgtcgcaaaagtagctttttcccctatatatacctataagtatggaggcatttgaggaaaaatttccacatagttttttccgaccttttgcccccatattccgcaggtctttatcgaaagaaagcttttacactgtttgccaactataagaggtagcaaatcagtactttagaagccattacgagcactcccatcctgtgcagagtcgagatcattgtttcttaggcaaaagtacactctgaccgacaacgtcaaaagatatagagccttagaaaccctttgattaggcttgatttccggcatagcaggagtggacgaagctatgccggtgcgaatgacacttgtgcccccttaccaatcggcacaccactcggcatacgcatactcgcatcccgatgccaacaaactcgaaccgccgaaaaaaggaatcgggcaccttcctcttcgacaaaaacacgaaccataaatggacaaaacatggttcgtgaatttcgagaaactcgagcctaaaaattattcttttggctcgcaaaaatttcacttctcaaaccaaaagcaaaattttcccgcgccacgtttttcagaaccacggatcacaatcttttggcaccacaaataaaactctcggagccatcggtctttttatctcagcaaggatggtaaaattcgaccgattacaacaatgccctaaacctttttcggcttagggcattgttggatagagtcatgtttttccgaggcagtcggaaggt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GACACAGAAUUAUUAUUCCAAUUUGUGUCGCAAAAGUAGCUUUUUCCCCUAUAUAUACCUAUAAGUAUGGAGGCAUUUGAGGAAAAAUUUCCACAUAGUUUUUUCCGACCUUUUGCCCCCAUAUUCCGCAGGUCUUUAUCGAAAGAAAGCUUUUACACUGUUUGCCAACUAUAAGAGGUAGCAAAUCAGUACUUUAGAAGCCAUUACGAGCACUCCCAUCCUGUGCAGAGUCGAGAUCAUUGUUUCUUAGGCAAAAGUACACUCUGACCGACAACGUCAAAAGAUAUAGAGCCUUAGAAACCCUUUGAUUAGGCUUGAUUUCCGGCAUAGCAGGAGUGGACGAAGCUAUGCCGGUGCGAAUGACACUUGUGCCCCCUUACCAAUCGGCACACCACUCGGCAUACGCAUACUCGCAUCCCGAUGCCAACAAACUCGAACCGCCGAAAAAAGGAAUCGGGCACCUUCCUCUUCGACAAAAACACGAACCAUAAAUGGACAAAACAUGGUUCGUGAAUUUCGAGAAACUCGAGCCUAAAAAUUAUUCUUUUGGCUCGCAAAAAUUUCACUUCUCAAACCAAAAGCAAAAUUUUCCCGCGCCACGUUUUUCAGAACCACGGAUCACAAUCUUUUGGCACCACAAAUAAAACUCUCGGAGCCAUCGGUCUUUUUAUCUCAGCAAGGAUGGUAAAAUUCGACCGAUUACAACAAUGCCCUAAACCUUUUUCGGCUUAGGGCAUUGUUGGAUAGAGUCAUGUUUUUCCGAGGCAGUCGGAAGGU ((((((((.............))))))))......(((.(((((...((((............(((((((.((((.....(((((((............))))))).......)))).))))))).....((((((..((....))..(((((((.(((((((((..(((......))).))))).))))....)))))))........((((.........))))......)))))).........)))).))))))))..(((..(((((...((((((((((...(((((..((((.....((..((((((((((.....(((((....(((((((.....((((((((((((.(.........((((((............)))))))))).))))))).))....)))).)))..)))))......((((((..(.((((...(((((..((...))))))).)))).).....((((((((((.............))))))))))...))))))....)))))))))).))...))))...)))))...(((((((..(((..........)))..))))))).(((.(....((((....))))).)))......))))))))))..............((((((((..(((((((.(((((((((......)).)))))))...)))))))..((((((((((((((.((......)).))))))))))))))...............))))))))..))))).))). (-227.24) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACCUUCCGACUGCCUCGGAAAAACAUGACUCUAUCCAACAAUGCCCUAAGCCGAAAAAGGUUUAGGGCAUUGUUGUAAUCGGUCGAAUUUUACCAUCCUUGCUGAGAUAAAAAGACCGAUGGCUCCGAGAGUUUUAUUUGUGGUGCCAAAAGAUUGUGAUCCGUGGUUCUGAAAAACGUGGCGCGGGAAAAUUUUGCUUUUGGUUUGAGAAGUGAAAUUUUUGCGAGCCAAAAGAAUAAUUUUUAGGCUCGAGUUUCUCGAAAUUCACGAACCAUGUUUUGUCCAUUUAUGGUUCGUGUUUUUGUCGAAGAGGAAGGUGCCCGAUUCCUUUUUUCGGCGGUUCGAGUUUGUUGGCAUCGGGAUGCGAGUAUGCGUAUGCCGAGUGGUGUGCCGAUUGGUAAGGGGGCACAAGUGUCAUUCGCACCGGCAUAGCUUCGUCCACUCCUGCUAUGCCGGAAAUCAAGCCUAAUCAAAGGGUUUCUAAGGCUCUAUAUCUUUUGACGUUGUCGGUCAGAGUGUACUUUUGCCUAAGAAACAAUGAUCUCGACUCUGCACAGGAUGGGAGUGCUCGUAAUGGCUUCUAAAGUACUGAUUUGCUACCUCUUAUAGUUGGCAAACAGUGUAAAAGCUUUCUUUCGAUAAAGACCUGCGGAAUAUGGGGGCAAAAGGUCGGAAAAAACUAUGUGGAAAUUUUUCCUCAAAUGCCUCCAUACUUAUAGGUAUAUAUAGGGGAAAAAGCUACUUUUGCGACACAAAUUGGAAUAAUAAUUCUGUGUC ...((((((.....)))))).....(((((.....(((((((((((((((((......))))))))))))))))).....))))).......((((((((((.(((.........((..((((((((((((((((((((.(.(.((((((((...((..((((((.(.(........).).))))))...))....)))))))).).).)))))))))))))).))))))............(((((((.((......(((((..(((((((((((...........))))))))))).))))).((((((.(...((((((....((((...((((((..((..(((....))).((((.(((((......))))).))))..))..)))))).....))))))))))...).)).))))..((((((((((.............))))))))))...)).))))))).....))((((((.((((....(((.(((((((.......))))))).))).....)))).))))))...........))).))).)))))))..(((.((((((.((.........(((((.(((((((.((.....)).))))))))))))....((((((.(..(.(((...(((((..((.((((((((((...(((........))).((.((((....)))).))..)))))))))).)).)))))...)))..)..).)))))).)).))))))))).....(((((....)))))..... (-272.62) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 930 140 91 231 182 NC_002950:PG2182|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_A_2291155..2291385_NEGATIVE 617 610 561 123 74 NC_002950:PG1980|5end_hypothetical_protein_2067930..2068160_NEGATIVE 578 609 566 99 57 NC_002950:PG1799|5end_hypothetical_protein_1894499..1894729_POSITIVE 427 400 352 64 23 NC_002950:PG0633|5end_hypothetical_protein_685301..685531_POSITIVE 425 359 312 193 131 NC_002950:PG1815|5end_3-deoxy-manno-octulosonate_cytidylyltransferase_1911433..1911663_NEGATIVE 419 618 575 47 1 NC_002950:PG0524|5end_hypothetical_protein_568599..568829_POSITIVE 415 359 311 214 163 NC_002950:PG1485|5end_conjugative_transposon_protein_TraC_1560370..1560600_NEGATIVE 404 606 565 83 42 NC_002950:PG2064|5end_hypothetical_protein_2160550..2160780_POSITIVE 400 360 312 62 14 NC_002950:PG1998|5end_polyprenyl_synthetase_2088029..2088259_NEGATIVE 391 356 312 65 23 NC_002950:PG0217|5end_hypothetical_protein_252490..252720_POSITIVE 390 380 332 183 133 NC_002950:PG1418|5end_DNA_polymerase_III,_gamma_and_tau_subunits_1503064..1503294_POSITIVE 389 390 341 60 6 NC_002950:PG1678|5end_hypothetical_protein_1763862..1764092_NEGATIVE 389 359 311 215 152 NC_002950:PG1348|5end_conserved_hypothetical_protein_TIGR00147_1428608..1428838_POSITIVE 385 420 374 205 149 NC_002950:PG1588|5end_hypothetical_protein_1666532..1666762_NEGATIVE 384 400 352 57 1 NC_002950:PG0302|5end_hypothetical_protein_338219..338449_POSITIVE 380 369 322 50 7 NC_002950:PG1966|5end_hypothetical_protein_2056924..2057154_NEGATIVE 377 360 314 46 7 NC_002950:PG1487|5end_hypothetical_protein_1561468..1561698_POSITIVE 377 360 313 230 182 NC_002950:PG0091|5end_transporter,_putative_107400..107630_NEGATIVE 375 358 311 62 11 NC_002950:PG2159|5end_protoporphyrinogen_oxidase_2264397..2264627_POSITIVE 375 248 201 200 153 NC_002950:PG2028|5end_ebsC_protein_2126027..2126257_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 427 400 352 59 18 NC_002950:PG0632|3end_biopolymer_transport_protein_ExbD,_putative_685376..685526_POSITIVE 410 360 311 68 15 NC_002950:PG0226|3end_transglutaminase-related_protein_259838..259988_NEGATIVE 390 380 332 60 10 NC_002950:PG1417|3end_fumarate_hydratase_class_I,_anaerobic_1503021..1503171_POSITIVE 390 626 583 112 71 NC_002950:PG0732|3end_hypothetical_protein_783018..783168_POSITIVE 385 420 374 144 88 NC_002950:PG1589|3end_dihydropteroate_synthase_1666593..1666743_NEGATIVE 380 357 312 136 84 NC_002950:PG0020|3end_transcriptional_regulator,_MarR_family_24166..24316_POSITIVE 375 358 311 58 7 NC_002950:PG2158|3end_hypothetical_protein_2264473..2264623_POSITIVE 370 618 573 72 29 NC_002950:PG1626|3end_hypothetical_protein_1710486..1710636_POSITIVE 367 458 411 80 26 NC_002950:PG1653|3end_hypothetical_protein_1736458..1736608_POSITIVE 366 400 351 136 84 NC_002950:PG0555|3end_DNA-binding_protein,_histone-like_family_611716..611866_NEGATIVE 365 770 726 110 66 NC_002950:PG1766|3end_phosphoribosylglycinamide_formyltransferase_1854078..1854228_POSITIVE 364 357 313 91 47 NC_002950:PG0334|3end_glycosyl_transferase,_group_2_family_protein_368792..368942_NEGATIVE 363 358 311 66 27 NC_002950:PG0066|3end_hypothetical_protein_81264..81414_NEGATIVE 360 363 322 116 54 NC_002950:PG0767|3end_4-alpha-glucanotransferase_818557..818707_NEGATIVE 353 357 311 133 81 NC_002950:PG0164|3end_hypothetical_protein_187081..187231_NEGATIVE 353 357 315 141 95 NC_002950:PG0033|3end_RmuC_domain_protein_40242..40392_NEGATIVE 352 358 314 116 50 NC_002950:PG0488|3end_Holliday_junction_DNA_helicase_RuvB_529280..529430_POSITIVE 351 358 311 64 15 NC_002950:PG0142|3end_SpoOJ_regulator_protein_164755..164905_NEGATIVE 341 357 311 140 94 NC_002950:PG1690|3end_Sua5/YciO/YrdC/YwlC_family_protein_1775171..1775321_NEGATIVE 339 459 416 76 32 NC_002950:PG1391|3end_hypothetical_protein_1472961..1473111_POSITIVE