Origin IGS: aaaaggaattcctataatcttatccgtcacccttctcctgtaacaatcacagttatgggaagggtacttatttgccgcagtatatgaagtgcgagaagggggagtctctatagg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cctatagagactcccccttctcgcacttcatatactgcggcaaataagtacccttcccataactgtgattgttacaggagaagggtgacggataagattataggaattcctttt Mask Tandem Repeat Region ================================================ aaaaggaattcctataatcttatccgtcacccttctcctgtaacaatcacagttatgggaagggtacttatttgccgcagtatatgaagtgcgagaagggggagtctctatagg Find is-nt database================================================ Query_seq: PG2119:PG2120|PG2119:PG2120:oxidoreductase, Gfo/Idh/MocA family:metallo-beta-lactamase superfamily protein:-><-:2227659..2227772 114 aaaaggaattcctataatcttatccgtcacccttctcctgtaacaatcacagttatgggaagggtacttatttgccgcagtatatgaagtgcgagaagggggagtctctatagg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG2119:PG2120|PG2119:PG2120:oxidoreductase, Gfo/Idh/MocA family:metallo-beta-lactamase superfamily protein:-><-:2227659..2227772 114 aaaaggaattcctataatcttatccgtcacccttctcctgtaacaatcacagttatgggaagggtacttatttgccgcagtatatgaagtgcgagaagggggagtctctatagg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG2119:PG2120|PG2119:PG2120:oxidoreductase, Gfo/Idh/MocA family:metallo-beta-lactamase superfamily protein:-><-:2227659..2227772 114 aaaaggaattcctataatcttatccgtcacccttctcctgtaacaatcacagttatgggaagggtacttatttgccgcagtatatgaagtgcgagaagggggagtctctatagg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaaggaattcctataatcttatccgtcacccttctcctgtaacaatcacagttatgggaagggtacttatttgccgcagtatatgaagtgcgagaagggggagtctctatagg Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: - Start: 1 End: 111 F P I G I I K D T V R R R Y C D C N H S P Y K N A A T Y S T R S P P T E M ... aaaaggaattcctataatcttatccgtcacccttctcctgtaacaatcacagttatgggaagggtacttatttgccgcagtatatgaagtgcgagaagggggagtctctatagg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaaggaattcctataatcttatccgtcacccttctcctgtaacaatcacagttatgggaagggtacttatttgccgcagtatatgaagtgcgagaagggggagtctctatagg Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 42 HP_score: -2.3 Tail_Score: -3.02371 Start: 30 End: 64 Full_Region: TAATCTTATCCGTCA CCCTTCTCCTGTAAC AATCACA GTTATGGGAAGGG TACTTATTTGCCGCA .............................CCCTTCTCCTGTAACAATCACAGTTATGGGAAGGG.................................................. Find igs database================================================ Query_seq: PG2119:PG2120|PG2119:PG2120:oxidoreductase, Gfo/Idh/MocA family:metallo-beta-lactamase superfamily protein:-><-:2227659..2227772 114 aaaaggaattcctataatcttatccgtcacccttctcctgtaacaatcacagttatgggaagggtacttatttgccgcagtatatgaagtgcgagaagggggagtctctatagg Intra-Species Hit: Count: 1 Min: 1 Max: 114 Len: 114 Subject: NC_002950_PG2119_PG2120|oxidoreductase, Gfo/Idh/MocA family:metallo-beta-lactamase superfamily protein|POSITIVE:NEGATIVE|[2227659,2227772]|114 HSP 1 e-value: 1.0E-58 bit: 226.0 Len: 114 Query Start:1 Query End:114 Subject Strand: POSITIVE Subject Start: 1 Subject End: 114 aaaaggaattcctataatcttatccgtcacccttctcctgtaacaatcacagttatgggaagggtacttatttgccgcagtatatgaagtgcgagaagggggagtctctatagg aaaaggaattcctataatcttatccgtcacccttctcctgtaacaatcacagttatgggaagggtacttatttgccgcagtatatgaagtgcgagaagggggagtctctatagg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAGGAAUUCCUAUAAUCUUAUCCGUCACCCUUCUCCUGUAACAAUCACAGUUAUGGGAAGGGUACUUAUUUGCCGCAGUAUAUGAAGUGCGAGAAGGGGGAGUCUCUAUAGG ..........((((((......(((....(((((((((((((((.......)))))))))...((((((...(((....)))....))))))..))))))))).....)))))) (-31.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CCUAUAGAGACUCCCCCUUCUCGCACUUCAUAUACUGCGGCAAAUAAGUACCCUUCCCAUAACUGUGAUUGUUACAGGAGAAGGGUGACGGAUAAGAUUAUAGGAAUUCCUUUU (((((((....(((......(((((..........)))))........((((((((((.((((.......))))..)).))))))))..))).....))))))).......... (-28.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 342 99 54 50 4 NC_002950:PG0949|5end_hypothetical_protein_1008686..1008916_POSITIVE 335 107 62 180 136 NC_002950:PG1837|5end_hemagglutinin_protein_HagA_1930337..1930567_POSITIVE 330 109 62 53 2 NC_002950:PG1791|5end_hypothetical_protein_1881166..1881396_NEGATIVE 321 109 64 52 1 NC_002950:PG0932|5end_DNA_polymerase_III,_delta_prime_subunit,_putative_988503..988733_POSITIVE 315 93 51 194 152 NC_002950:PG0071|5end_UDP-3-O-acyl-GlcNAc_deacetylase/beta-hydroxyacyl-[acyl_carrier_protein]_dehydratase_FabZ_86119..86349_NEGATIVE 302 109 75 198 150 NC_002950:PG1739|5end_hypothetical_protein_1825709..1825939_NEGATIVE 300 100 55 102 50 NC_002950:PG0153|5end_aspartyl-tRNA_synthetase_177430..177660_NEGATIVE 299 106 77 182 159 NC_002950:PG0085|5end_alpha-galactosidase_99172..99402_POSITIVE 292 99 55 206 156 NC_002950:PG0332|5end_transcription_termination_factor_Rho_365619..365849_POSITIVE 291 108 61 223 160 NC_002950:PG1576|5end_L-aspartate_oxidase_1653016..1653246_POSITIVE 287 102 62 179 137 NC_002950:PG1495|5end_DNA_topoisomerase_III_1571243..1571473_POSITIVE 286 104 62 50 10 NC_002950:PG0924|5end_5'-nucleotidase,_lipoprotein_e(P4)_family_983668..983898_NEGATIVE 285 99 58 138 96 NC_002950:PG0664|5end_oxidoreductase,_Gfo/Idh/MocA_family_710293..710523_POSITIVE 285 78 31 189 137 NC_002950:PG0634|5end_ThiJ/PfpI_family_protein_686143..686373_POSITIVE 284 109 64 176 131 NC_002950:PG0545|5end_hypothetical_protein_598043..598273_POSITIVE 283 104 61 230 199 NC_002950:PG1099|5end_hypothetical_protein_1168168..1168398_NEGATIVE 283 59 13 184 131 NC_002950:PG0312|5end_hypothetical_protein_347048..347278_NEGATIVE 283 69 23 172 136 NC_002950:PG0046|5end_phosphatidate_cytidylyltransferase_57200..57430_NEGATIVE 279 108 68 174 125 NC_002950:PG1993|5end_excinuclease_ABC,_C_subunit_2083321..2083551_POSITIVE 279 104 62 73 34 NC_002950:PG1283|5end_hypothetical_protein_1360553..1360783_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 388 109 63 91 38 NC_002950:PG1723|3end_ribosomal_protein_S20_1813403..1813553_POSITIVE 348 68 22 147 96 NC_002950:PG0555|3end_DNA-binding_protein,_histone-like_family_611716..611866_NEGATIVE 317 78 31 55 5 NC_002950:PG2136|3end_hypothetical_protein_2245104..2245254_POSITIVE 307 105 74 91 58 NC_002950:PG1599|3end_hypothetical_protein_1679955..1680105_POSITIVE 303 109 63 48 2 NC_002950:PG2145|3end_polysaccharide_deacetylase_2253938..2254088_POSITIVE 302 109 75 51 3 NC_002950:PG1741|3end_aspartate_ammonia-lyase_1825856..1826006_NEGATIVE 299 106 77 41 18 NC_002950:PG0084|3end_L-serine_dehydratase,_iron-sulfur-dependent,_single_chain_form_99111..99261_POSITIVE 293 93 54 123 76 NC_002950:PG2167|3end_immunoreactive_53_kDa_antigen_PG123_2272104..2272254_NEGATIVE 289 109 61 84 38 NC_002950:PG1808|3end_guanosine-3',5'-bis(diphosphate)_3'-pyrophosphohydrolase_1902955..1903105_NEGATIVE 289 79 32 51 2 NC_002950:PG0340|3end_hypothetical_protein_376436..376586_POSITIVE 288 104 61 128 83 NC_002950:PG0932|3end_DNA_polymerase_III,_delta_prime_subunit,_putative_989833..989983_POSITIVE 287 102 62 90 48 NC_002950:PG1494|3end_hypothetical_protein_1571234..1571384_POSITIVE 286 104 62 53 13 NC_002950:PG0925|3end_thymidine_kinase_983665..983815_NEGATIVE 286 65 23 80 44 NC_002950:PG0769|3end_fibronectin_type_III_domain_protein_822555..822705_NEGATIVE 284 109 64 131 86 NC_002950:PG0544|3end_type_I_restriction-modification_system,_M_subunit,_putative_598078..598228_POSITIVE 283 69 23 143 107 NC_002950:PG0047|3end_cell_division_protein_FtsH,_putative_57229..57379_NEGATIVE 280 108 66 92 47 NC_002950:PG1722|3end_hypothetical_protein_1812633..1812783_NEGATIVE 279 108 68 140 91 NC_002950:PG1992|3end_glucose-inhibited_division_protein_A_2083367..2083517_POSITIVE 277 106 61 92 46 NC_002950:PG1353|3end_orotate_phosphoribosyltransferase_1433201..1433351_NEGATIVE 277 107 62 58 10 NC_002950:PG1041|3end_K+-dependent_Na+/Ca+_exchanger_related-protein_1107970..1108120_NEGATIVE