Origin IGS: ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc Mask Tandem Repeat Region ================================================ ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat Find is-nt database================================================ Query_seq: PG2073:PG2074|PG2073:PG2074:hypothetical protein:hypothetical protein:<-<-:2174465..2174592 128 ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG2073:PG2074|PG2073:PG2074:hypothetical protein:hypothetical protein:<-<-:2174465..2174592 128 ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG2073:PG2074|PG2073:PG2074:hypothetical protein:hypothetical protein:<-<-:2174465..2174592 128 ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 37 End: 126 .................................... M S P S S I Q E R E N T L P D I P L D C I G I S A G Y I R F .. Protein_Len: 30 Strand: - Start: 28 End: 117 ........................... R G T D R W R R N L L S L V G E R V D R K I A D S Y G C T M ........... Protein_Len: 37 Strand: - Start: 2 End: 112 . G R P L S L S R I K R Y R E M K S E L S L S C G G P C G A Q N C R F I R M ................ ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG2073:PG2074|PG2073:PG2074:hypothetical protein:hypothetical protein:<-<-:2174465..2174592 128 ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat Intra-Species Hit: Count: 4 Min: 1 Max: 128 Len: 128 Subject: NC_002950_PG2073_PG2074|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[2174465,2174592]|128 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:1 Query End:128 Subject Strand: POSITIVE Subject Start: 1 Subject End: 128 ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat Subject: NC_002950_PG1669_PG1670|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1755657,1755784]|128 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:1 Query End:128 Subject Strand: POSITIVE Subject Start: 1 Subject End: 128 ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat Subject: NC_002950_PG1264_PG1265|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1344986,1345113]|128 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:1 Query End:128 Subject Strand: POSITIVE Subject Start: 1 Subject End: 128 ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat Subject: NC_002950_PG0102_PG0103|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[121302,121429]|128 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:1 Query End:128 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 128 ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGCCUCGGGGGAGGGAAAGACUCCUUAUCUUCCUGUAUCUCUCCAUCUUCGAUUCAAGAGAGAGAGAACACCCUCCCGGACAUCCCGCUUGAUUGCAUCGGAAUAUCCGCAGGUUAUAUUCGUUUUAU (((((((((((((((.((((.......))))......((((((..((((......))))..))))))...))))))).....(((.((......))...)))....))).)))))............. (-39.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAAACGAAUAUAACCUGCGGAUAUUCCGAUGCAAUCAAGCGGGAUGUCCGGGAGGGUGUUCUCUCUCUCUUGAAUCGAAGAUGGAGAGAUACAGGAAGAUAAGGAGUCUUUCCCUCCCCCGAGGCC ...............(((..((((((((((...........))))))))))(((((((.(((((((((.((((......)))).))))))).))..((((((.....)))))))))))))...))).. (-45.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1063 110 61 201 152 NC_002950:PG2073|5end_hypothetical_protein_2174374..2174604_NEGATIVE 1063 110 61 201 152 NC_002950:PG1669|5end_hypothetical_protein_1755566..1755796_NEGATIVE 1063 110 61 201 152 NC_002950:PG1264|5end_hypothetical_protein_1344895..1345125_NEGATIVE 1063 110 61 201 152 NC_002950:PG0103|5end_hypothetical_protein_121290..121520_POSITIVE 444 77 31 231 179 NC_002950:PG0123|5end_hypothetical_protein_144987..145217_POSITIVE 388 60 13 199 145 NC_002950:PG1260|5end_anaerobic_ribonucleoside_triphosphate_reductase_1337894..1338124_NEGATIVE 352 48 3 126 82 NC_002950:PG1831|5end_ATP-dependent_DNA_helicase_RecQ_1925422..1925652_NEGATIVE 350 109 61 173 115 NC_002950:PG1536|5end_cell_division_protein_FtsX,_putative_1616362..1616592_POSITIVE 349 113 73 219 152 NC_002950:PG0282|5end_ABC_transporter,_ATP-binding_protein_316915..317145_NEGATIVE 348 110 75 54 15 NC_002950:PG1675|5end_hypothetical_protein_1760182..1760412_POSITIVE 338 110 61 177 120 NC_002950:PG0157|5end_regulatory_protein_RecX_179706..179936_POSITIVE 336 87 43 124 80 NC_002950:PG0563|5end_hypothetical_protein_621438..621668_NEGATIVE 335 49 1 94 42 NC_002950:PG0281|5end_ABC_transporter,_permease_protein,_putative_316023..316253_NEGATIVE 332 113 71 198 148 NC_002950:PG0986|5end_hypothetical_protein_1047426..1047656_POSITIVE 331 43 4 216 181 NC_002950:PG1008|5end_hypothetical_protein_1067644..1067874_NEGATIVE 330 50 3 148 94 NC_002950:PG0158|5end_competence_protein_F-related_protein_180175..180405_POSITIVE 330 44 1 125 86 NC_002950:PG0124|5end_hypothetical_protein_146234..146464_NEGATIVE 325 80 33 184 138 NC_002950:PG1688|5end_transcription_elongation_factor_GreA_1774686..1774916_NEGATIVE 322 88 47 40 7 NC_002950:PG0671|5end_iron_compound_ABC_transporter,_permease_protein_721888..722118_POSITIVE 321 115 71 74 30 NC_002950:PG1094|5end_phosphomannomutase_1158753..1158983_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1063 110 61 72 23 NC_002950:PG1670|3end_hypothetical_protein_1755695..1755845_NEGATIVE 1063 110 61 72 23 NC_002950:PG1265|3end_hypothetical_protein_1345024..1345174_NEGATIVE 1063 110 61 72 23 NC_002950:PG0102|3end_hypothetical_protein_121241..121391_POSITIVE 444 109 61 97 37 NC_002950:PG0232|3end_zinc_carboxypeptidase,_putative_267458..267608_NEGATIVE 382 50 1 101 43 NC_002950:PG1500|3end_hypothetical_protein_1578470..1578620_POSITIVE 373 111 71 151 111 NC_002950:PG2205|3end_2-dehydropantoate_2-reductase_2317145..2317295_POSITIVE 362 46 1 85 36 NC_002950:PG0143|3end_hydrolase,_carbon-nitrogen_family_165747..165897_NEGATIVE 349 113 73 139 72 NC_002950:PG0283|3end_efflux_transporter,_MFP_component,_RND_family_316995..317145_NEGATIVE 337 47 5 126 86 NC_002950:PG1276|3end_DNA-binding_protein,_histone-like_family_1353531..1353681_POSITIVE 337 47 5 126 86 NC_002950:PG0566|3end_DNA-binding_protein,_histone-like_family_622935..623085_NEGATIVE 336 87 43 146 102 NC_002950:PG0564|3end_hypothetical_protein_621416..621566_NEGATIVE 330 44 1 121 82 NC_002950:PG0125|3end_hypothetical_protein_146238..146388_NEGATIVE 322 88 47 43 10 NC_002950:PG0670|3end_lipoprotein,_putative_721971..722121_POSITIVE 320 43 8 53 9 NC_002950:PG2004|3end_hypothetical_protein_2093135..2093285_NEGATIVE 320 115 76 147 114 NC_002950:PG0180|3end_lipoprotein,_putative_204475..204625_POSITIVE 318 80 32 51 8 NC_002950:PG1862|3end_hypothetical_protein_1960070..1960220_NEGATIVE 315 50 1 67 11 NC_002950:PG0172|3end_exonuclease_194355..194505_POSITIVE 314 109 75 151 113 NC_002950:PG1675|3end_hypothetical_protein_1760360..1760510_POSITIVE 314 90 41 103 19 NC_002950:PG1497|3end_DNA-binding_protein,_histone-like_family_1574635..1574785_POSITIVE 307 113 71 144 87 NC_002950:PG0610|3end_hypothetical_protein_663604..663754_NEGATIVE