Origin IGS: gtggttatagcttataatatatttaatggcctaaagttactctaaaagctgtaattaatacgtatttttttatgcttatttgcgaggaaatcggatatatagcatccatcgttccgaggaattttgcgaaaaccgcactgaaaatagaaaaggggcgagggcggggtataaaatacgaaccgaaaatgctccgattgtggtgcgtgttttctgaaaaaccggaacaagaagtttttcgttgtggcgcgagaattttttatttctcgaaccaaaacgaaaaagtttacgcgccacgtttttcgggggcgcagacacagagaagtcggttcgtgaatgaaccgggtataacgaggtgtgccttatcgctttatgtgaacctttaacaagagatcaagacagccgtagcaagtctgtctttcaggtatttcatcttgtgaatagagaagggataatatcctctttcggagtattgggtaagtgggcgattcgaggaggttcgatcataagctcctatgattagagatacttcggttttgagataatgttagcgagacccgaatagagccaatcatgaataaaaggtttatcccaagatggagattcctacatggaactctctccgattgcgatagaaagggagtaggata .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tatcctactccctttctatcgcaatcggagagagttccatgtaggaatctccatcttgggataaaccttttattcatgattggctctattcgggtctcgctaacattatctcaaaaccgaagtatctctaatcataggagcttatgatcgaacctcctcgaatcgcccacttacccaatactccgaaagaggatattatcccttctctattcacaagatgaaatacctgaaagacagacttgctacggctgtcttgatctcttgttaaaggttcacataaagcgataaggcacacctcgttatacccggttcattcacgaaccgacttctctgtgtctgcgcccccgaaaaacgtggcgcgtaaactttttcgttttggttcgagaaataaaaaattctcgcgccacaacgaaaaacttcttgttccggtttttcagaaaacacgcaccacaatcggagcattttcggttcgtattttataccccgccctcgccccttttctattttcagtgcggttttcgcaaaattcctcggaacgatggatgctatatatccgatttcctcgcaaataagcataaaaaaatacgtattaattacagcttttagagtaactttaggccattaaatatattataagctataaccac Mask Tandem Repeat Region ================================================ gtggttatagcttataatatatttaatggcctaaagttactctaaaagctgtaattaatacgtatttttttatgcttatttgcgaggaaatcggatatatagcatccatcgttccgaggaattttgcgaaaaccgcactgaaaatagaaaaggggcgagggcggggtataaaatacgaaccgaaaatgctccgattgtggtgcgtgttttctgaaaaaccggaacaagaagtttttcgttgtggcgcgagaattttttatttctcgaaccaaaacgaaaaagtttacgcgccacgtttttcgggggcgcagacacagagaagtcggttcgtgaatgaaccgggtataacgaggtgtgccttatcgctttatgtgaacctttaacaagagatcaagacagccgtagcaagtctgtctttcaggtatttcatcttgtgaatagagaagggataatatcctctttcggagtattgggtaagtgggcgattcgaggaggttcgatcataagctcctatgattagagatacttcggttttgagataatgttagcgagacccgaatagagccaatcatgaataaaaggtttatcccaagatggagattcctacatggaactctctccgattgcgatagaaagggagtaggata Find is-nt database================================================ Query_seq: PG2020:PG2021|PG2020:PG2021:CRISPR-associated TM1814 family protein:hypothetical protein:<-<-:2112364..2113010 647 gtggttatagcttataatatatttaatggcctaaagttactctaaaagctgtaattaatacgtatttttttatgcttatttgcgaggaaatcggatatatagcatccatcgttccgaggaattttgcgaaaaccgcactgaaaatagaaaaggggcgagggcggggtataaaatacgaaccgaaaatgctccgattgtggtgcgtgttttctgaaaaaccggaacaagaagtttttcgttgtggcgcgagaattttttatttctcgaaccaaaacgaaaaagtttacgcgccacgtttttcgggggcgcagacacagagaagtcggttcgtgaatgaaccgggtataacgaggtgtgccttatcgctttatgtgaacctttaacaagagatcaagacagccgtagcaagtctgtctttcaggtatttcatcttgtgaatagagaagggataatatcctctttcggagtattgggtaagtgggcgattcgaggaggttcgatcataagctcctatgattagagatacttcggttttgagataatgttagcgagacccgaatagagccaatcatgaataaaaggtttatcccaagatggagattcctacatggaactctctccgattgcgatagaaagggagtaggata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG2020:PG2021|PG2020:PG2021:CRISPR-associated TM1814 family protein:hypothetical protein:<-<-:2112364..2113010 647 gtggttatagcttataatatatttaatggcctaaagttactctaaaagctgtaattaatacgtatttttttatgcttatttgcgaggaaatcggatatatagcatccatcgttccgaggaattttgcgaaaaccgcactgaaaatagaaaaggggcgagggcggggtataaaatacgaaccgaaaatgctccgattgtggtgcgtgttttctgaaaaaccggaacaagaagtttttcgttgtggcgcgagaattttttatttctcgaaccaaaacgaaaaagtttacgcgccacgtttttcgggggcgcagacacagagaagtcggttcgtgaatgaaccgggtataacgaggtgtgccttatcgctttatgtgaacctttaacaagagatcaagacagccgtagcaagtctgtctttcaggtatttcatcttgtgaatagagaagggataatatcctctttcggagtattgggtaagtgggcgattcgaggaggttcgatcataagctcctatgattagagatacttcggttttgagataatgttagcgagacccgaatagagccaatcatgaataaaaggtttatcccaagatggagattcctacatggaactctctccgattgcgatagaaagggagtaggata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG2020:PG2021|PG2020:PG2021:CRISPR-associated TM1814 family protein:hypothetical protein:<-<-:2112364..2113010 647 gtggttatagcttataatatatttaatggcctaaagttactctaaaagctgtaattaatacgtatttttttatgcttatttgcgaggaaatcggatatatagcatccatcgttccgaggaattttgcgaaaaccgcactgaaaatagaaaaggggcgagggcggggtataaaatacgaaccgaaaatgctccgattgtggtgcgtgttttctgaaaaaccggaacaagaagtttttcgttgtggcgcgagaattttttatttctcgaaccaaaacgaaaaagtttacgcgccacgtttttcgggggcgcagacacagagaagtcggttcgtgaatgaaccgggtataacgaggtgtgccttatcgctttatgtgaacctttaacaagagatcaagacagccgtagcaagtctgtctttcaggtatttcatcttgtgaatagagaagggataatatcctctttcggagtattgggtaagtgggcgattcgaggaggttcgatcataagctcctatgattagagatacttcggttttgagataatgttagcgagacccgaatagagccaatcatgaataaaaggtttatcccaagatggagattcctacatggaactctctccgattgcgatagaaagggagtaggata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 4 Min: 141 Max: 345 Len: 205 Subject: UniRef90_Q7MU89 Cluster: Hypothetical protein; n=1; Porphyromonas gingivalis|Rep: Hypothetical protein - Porphyromonas gingivalis (Bacteroides gingivalis) HSP 1 e-value: 2.0E-9 bit: 65.1 Len: 174 Query Start:172 Query End:345 Subject Strand: null Subject Start: 12 Subject End: 69 ........................................................................................................................................................................... N T N R K C S D C G A C F L K N R N K K F F V V A R E F F I S R T K T K K F T R H V F R G R R H R E V G S * M N R V .............................................................................................................................................................................................................................................................................................................. ........................................................................................................................................................................... N T N Q K S S D C G S C F L K K W R E I F F V L A C K F F N C R A K T K K F S R H V F V G N K C E N S G T Q T Y R I .............................................................................................................................................................................................................................................................................................................. Subject: UniRef90_Q7MUH1 Cluster: Hypothetical protein; n=1; Porphyromonas gingivalis|Rep: Hypothetical protein - Porphyromonas gingivalis (Bacteroides gingivalis) HSP 1 e-value: 2.0E-9 bit: 64.7 Len: 138 Query Start:172 Query End:309 Subject Strand: null Subject Start: 17 Subject End: 62 ........................................................................................................................................................................... A P P K N V A R K L F R F G S R N K K F S R H N E K L L V P V F Q K T R T T I G A F S V R I .................................................................................................................................................................................................................................................................................................................................................. ........................................................................................................................................................................... A T P Q N A V R K L F Y F G V G S K K F S R H N E K I L A P L F Q G I R A T I G A F P V R V .................................................................................................................................................................................................................................................................................................................................................. Subject: UniRef90_Q7MTB1 Cluster: Hypothetical protein; n=1; Porphyromonas gingivalis|Rep: Hypothetical protein - Porphyromonas gingivalis (Bacteroides gingivalis) HSP 1 e-value: 6.0E-8 bit: 60.1 Len: 159 Query Start:141 Query End:299 Subject Strand: null Subject Start: 4 Subject End: 56 ............................................................................................................................................ K T W R V N F F V L V R E I K N S R A T T K N F L F R F F R K H A P Q S E H F R F V F Y T P P S P L F Y F ............................................................................................................................................................................................................................................................................................................................................................ ............................................................................................................................................ K T W R E N F F V L V R E V K N L R A T A K K I S R H F F R K Y E P Q P G V F W F V F H L S R S H P F P F ............................................................................................................................................................................................................................................................................................................................................................ Subject: UniRef90_Q7MXG1 Cluster: Hypothetical protein; n=1; Porphyromonas gingivalis|Rep: Hypothetical protein - Porphyromonas gingivalis (Bacteroides gingivalis) HSP 1 e-value: 7.0E-6 bit: 53.1 Len: 123 Query Start:174 Query End:296 Subject Strand: null Subject Start: 70 Subject End: 110 ............................................................................................................................................................................. Y E P K M L R L W C V F S E K P E Q E V F R C G A R I F Y F S N Q N E K V Y A P R ............................................................................................................................................................................................................................................................................................................................................................... ............................................................................................................................................................................. Y E P K T V G L R C V F W R K H A R E I F R F G S K N F Y F S R H N E K I F A P R ............................................................................................................................................................................................................................................................................................................................................................... gtggttatagcttataatatatttaatggcctaaagttactctaaaagctgtaattaatacgtatttttttatgcttatttgcgaggaaatcggatatatagcatccatcgttccgaggaattttgcgaaaaccgcactgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaacgaggtgtgccttatcgctttatgtgaacctttaacaagagatcaagacagccgtagcaagtctgtctttcaggtatttcatcttgtgaatagagaagggataatatcctctttcggagtattgggtaagtgggcgattcgaggaggttcgatcataagctcctatgattagagatacttcggttttgagataatgttagcgagacccgaatagagccaatcatgaataaaaggtttatcccaagatggagattcctacatggaactctctccgattgcgatagaaagggagtaggata Predict ORF larger than 30AA ================================================ Protein_Len: 44 Strand: + Start: 80 End: 211 ............................................................................... M R G N R I Y S I H R S E E F C E N R T E N R K G A R A G Y K I R T E N A P I V V R V F .................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 40 Strand: + Start: 211 End: 330 .................................................................................................................................................................................................................. M K N R N K K F F V V A R E F F I S R T K T K K F T R H V F R G R R H R E V G S ............................................................................................................................................................................................................................................................................................................................. Protein_Len: 32 Strand: + Start: 239 End: 334 .............................................................................................................................................................................................................................................. M W R E N F L F L E P K R K S L R A T F F G G A D T E K S V R E ......................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 54 End: 380 ..................................................... M N T Y F F M L I C E E I G Y I A S I V P R N F A K T A L K I E K G R G R G I K Y E P K M L R L W C V F S E K P E Q E V ........................................................................................................................................................................................................................................................................... Protein_Len: 55 Strand: + Start: 353 End: 517 ................................................................................................................................................................................................................................................................................................................................................................ M C L I A L C E P L T R D Q D S R S K S V F Q V F H L V N R E G I I S S F G V L G K W A I R G G S I I S S Y D .................................................................................................................................. Protein_Len: 45 Strand: - Start: 383 End: 517 .............................................................................................................................................................................................................................................................................................................................................................................................. C S I L V A T A L R D K L Y K M K H I S F P Y Y G R E S Y Q T L P R N S S T R D Y A G I M .................................................................................................................................. Protein_Len: 36 Strand: - Start: 363 End: 470 .......................................................................................................................................................................................................................................................................................................................................................................... R K I H V K L L L D L C G Y C T Q R E P I E D Q S Y L L S L I R K R L M ................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 79 End: 363 .............................................................................. F A G I T T R T K Q F V P V L L K E N H R S F K K N R S G F R F L K R A V N K P P A S V S F D T R S H V P Y L S T H R M ............................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: - Start: 45 End: 335 ............................................ S P P T Y F V F R F H E S Q P A H K R F F R F L F N K T T A R S N K I E R V L V F F N V R W T K R P R L C L S T P E H M ........................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: - Start: 26 End: 253 ......................... S I Q S S I P Y I A D M T G L F K A F V A S F I S F P R P R P I F Y S G F I S R N H H T N E S F G S C S T K R Q P A L M .......................................................................................................................................................................................................................................................................................................................................................................................................... gtggttatagcttataatatatttaatggcctaaagttactctaaaagctgtaattaatacgtatttttttatgcttatttgcgaggaaatcggatatatagcatccatcgttccgaggaattttgcgaaaaccgcactgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaacgaggtgtgccttatcgctttatgtgaacctttaacaagagatcaagacagccgtagcaagtctgtctttcaggtatttcatcttgtgaatagagaagggataatatcctctttcggagtattgggtaagtgggcgattcgaggaggttcgatcataagctcctatgattagagatacttcggttttgagataatgttagcgagacccgaatagagccaatcatgaataaaaggtttatcccaagatggagattcctacatggaactctctccgattgcgatagaaagggagtaggata Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gtggttatagcttataatatatttaatggcctaaagttactctaaaagctgtaattaatacgtatttttttatgcttatttgcgaggaaatcggatatatagcatccatcgttccgaggaattttgcgaaaaccgcactgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaacgaggtgtgccttatcgctttatgtgaacctttaacaagagatcaagacagccgtagcaagtctgtctttcaggtatttcatcttgtgaatagagaagggataatatcctctttcggagtattgggtaagtgggcgattcgaggaggttcgatcataagctcctatgattagagatacttcggttttgagataatgttagcgagacccgaatagagccaatcatgaataaaaggtttatcccaagatggagattcctacatggaactctctccgattgcgatagaaagggagtaggata Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 83 HP_score: -9.6 Tail_Score: -3.97741 Start: 231 End: 282 Full_Region: aacgtggcgcgtaaa ctttttcgttttggttcgagaaat aaaa aattctcgcgccacaacgaaaaac ttcttgttccggttt ......................................................................................................................................................................................................................................ctttttcgttttggttcgagaaataaaaaattctcgcgccacaacgaaaaac............................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 95 HP_score: -13.1 Tail_Score: -2.9756 Start: 232 End: 281 Full_Region: acgtggcgcgtaaac tttttcgttttggttcgagaaat aaaa aattctcgcgccacaacgaaaaa cttcttgttccggtt .......................................................................................................................................................................................................................................tttttcgttttggttcgagaaataaaaaattctcgcgccacaacgaaaaa.............................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 76 HP_score: -10.9 Tail_Score: -2.71791 Start: 232 End: 281 Full_Region: AACCGGAACAAGAAG TTTTTCGTTGTGGCGCGAGAATT TTTT ATTTCTCGAACCAAAACGAAAAA GTTTACGCGCCACGT .......................................................................................................................................................................................................................................TTTTTCGTTGTGGCGCGAGAATTTTTTATTTCTCGAACCAAAACGAAAAA.............................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 66 HP_score: -4.1 Tail_Score: -4.56597 Start: 240 End: 295 Full_Region: CAAGAAGTTTTTCGT TGTGGCGCGAGAATTTTTTATTTCT CGAACC AAAACGAAAAAGTTTACGCGCCACG TTTTTCGGGGGCGCA ...............................................................................................................................................................................................................................................TGTGGCGCGAGAATTTTTTATTTCTCGAACCAAAACGAAAAAGTTTACGCGCCACG................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 55 HP_score: -5.4 Tail_Score: -3.33744 Start: 241 End: 294 Full_Region: AAGAAGTTTTTCGTT GTGGCGCGAGAATTTTTTATTTCT CGAACC AAAACGAAAAAGTTTACGCGCCAC GTTTTTCGGGGGCGC ................................................................................................................................................................................................................................................GTGGCGCGAGAATTTTTTATTTCTCGAACCAAAACGAAAAAGTTTACGCGCCAC................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 73 HP_score: -2.9 Tail_Score: -5.13016 Start: 282 End: 313 Full_Region: aaccgacttctctgt gtctgcgcccccg aaaaa cgtggcgcgtaaac tttttcgttttggtt .........................................................................................................................................................................................................................................................................................gtctgcgcccccgaaaaacgtggcgcgtaaac.............................................................................................................................................................................................................................................................................................................................................. Find igs database================================================ Query_seq: PG2020:PG2021|PG2020:PG2021:CRISPR-associated TM1814 family protein:hypothetical protein:<-<-:2112364..2113010 647 gtggttatagcttataatatatttaatggcctaaagttactctaaaagctgtaattaatacgtatttttttatgcttatttgcgaggaaatcggatatatagcatccatcgttccgaggaattttgcgaaaaccgcactgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaacgaggtgtgccttatcgctttatgtgaacctttaacaagagatcaagacagccgtagcaagtctgtctttcaggtatttcatcttgtgaatagagaagggataatatcctctttcggagtattgggtaagtgggcgattcgaggaggttcgatcataagctcctatgattagagatacttcggttttgagataatgttagcgagacccgaatagagccaatcatgaataaaaggtttatcccaagatggagattcctacatggaactctctccgattgcgatagaaagggagtaggata Intra-Species Hit: Count: 1 Min: 1 Max: 647 Len: 647 Subject: NC_002950_PG2020_PG2021|CRISPR-associated TM1814 family protein:hypothetical protein|NEGATIVE:NEGATIVE|[2112364,2113010]|647 HSP 1 e-value: 1.0E-170 bit: 599.0 Len: 302 Query Start:346 Query End:647 Subject Strand: POSITIVE Subject Start: 346 Subject End: 647 .........................................................................................................................................................................................................................................................................................................................................................taacgaggtgtgccttatcgctttatgtgaacctttaacaagagatcaagacagccgtagcaagtctgtctttcaggtatttcatcttgtgaatagagaagggataatatcctctttcggagtattgggtaagtgggcgattcgaggaggttcgatcataagctcctatgattagagatacttcggttttgagataatgttagcgagacccgaatagagccaatcatgaataaaaggtttatcccaagatggagattcctacatggaactctctccgattgcgatagaaagggagtaggata .........................................................................................................................................................................................................................................................................................................................................................taacgaggtgtgccttatcgctttatgtgaacctttaacaagagatcaagacagccgtagcaagtctgtctttcaggtatttcatcttgtgaatagagaagggataatatcctctttcggagtattgggtaagtgggcgattcgaggaggttcgatcataagctcctatgattagagatacttcggttttgagataatgttagcgagacccgaatagagccaatcatgaataaaaggtttatcccaagatggagattcctacatggaactctctccgattgcgatagaaagggagtaggata HSP 2 e-value: 3.0E-60 bit: 234.0 Len: 139 Query Start:1 Query End:139 Subject Strand: POSITIVE Subject Start: 1 Subject End: 139 gtggttatagcttataatatatttaatggcctaaagttactctaaaagctgtaattaatacgtannnnnnnatgcttatttgcgaggaaatcggatatatagcatccatcgttccgaggaattttgcgaaaaccgcact............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ gtggttatagcttataatatatttaatggcctaaagttactctaaaagctgtaattaatacgtatttttttatgcttatttgcgaggaaatcggatatatagcatccatcgttccgaggaattttgcgaaaaccgcact............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUGGUUAUAGCUUAUAAUAUAUUUAAUGGCCUAAAGUUACUCUAAAAGCUGUAAUUAAUACGUAUUUUUUUAUGCUUAUUUGCGAGGAAAUCGGAUAUAUAGCAUCCAUCGUUCCGAGGAAUUUUGCGAAAACCGCACUGAAAAUAGAAAAGGGGCGAGGGCGGGGUAUAAAAUACGAACCGAAAAUGCUCCGAUUGUGGUGCGUGUUUUCUGAAAAACCGGAACAAGAAGUUUUUCGUUGUGGCGCGAGAAUUUUUUAUUUCUCGAACCAAAACGAAAAAGUUUACGCGCCACGUUUUUCGGGGGCGCAGACACAGAGAAGUCGGUUCGUGAAUGAACCGGGUAUAACGAGGUGUGCCUUAUCGCUUUAUGUGAACCUUUAACAAGAGAUCAAGACAGCCGUAGCAAGUCUGUCUUUCAGGUAUUUCAUCUUGUGAAUAGAGAAGGGAUAAUAUCCUCUUUCGGAGUAUUGGGUAAGUGGGCGAUUCGAGGAGGUUCGAUCAUAAGCUCCUAUGAUUAGAGAUACUUCGGUUUUGAGAUAAUGUUAGCGAGACCCGAAUAGAGCCAAUCAUGAAUAAAAGGUUUAUCCCAAGAUGGAGAUUCCUACAUGGAACUCUCUCCGAUUGCGAUAGAAAGGGAGUAGGAUA ..((((((.................)))))).....(((((((....((((((.......((.((((((((..((......))))))))))))....)))))).((.(((((...(((((....((((.....))))(((((......(((((.(((((((((.(((.((...((((((((((.....((((((.((((((((((...(((((......)))))...(((.(((((((((.(((..((((((........))))))..))).))))))))).)))))))))))))....))))))..(......).......))))))))))..)).))).((((((......))))))....)))))).)))...)))))............((((((.(.......).))))))))))).(((..(.((((.......)))).)..)))...)))))..((((((..(((((......(((.(((((.((((.((((((((((......))))))).)))...))))(((((((..(......)..))))))))))))...)))....((((((.....)))))))))))...((((.((((.....))))))))))))))..))))).))...))))))).... (-189.83) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUCCUACUCCCUUUCUAUCGCAAUCGGAGAGAGUUCCAUGUAGGAAUCUCCAUCUUGGGAUAAACCUUUUAUUCAUGAUUGGCUCUAUUCGGGUCUCGCUAACAUUAUCUCAAAACCGAAGUAUCUCUAAUCAUAGGAGCUUAUGAUCGAACCUCCUCGAAUCGCCCACUUACCCAAUACUCCGAAAGAGGAUAUUAUCCCUUCUCUAUUCACAAGAUGAAAUACCUGAAAGACAGACUUGCUACGGCUGUCUUGAUCUCUUGUUAAAGGUUCACAUAAAGCGAUAAGGCACACCUCGUUAUACCCGGUUCAUUCACGAACCGACUUCUCUGUGUCUGCGCCCCCGAAAAACGUGGCGCGUAAACUUUUUCGUUUUGGUUCGAGAAAUAAAAAAUUCUCGCGCCACAACGAAAAACUUCUUGUUCCGGUUUUUCAGAAAACACGCACCACAAUCGGAGCAUUUUCGGUUCGUAUUUUAUACCCCGCCCUCGCCCCUUUUCUAUUUUCAGUGCGGUUUUCGCAAAAUUCCUCGGAACGAUGGAUGCUAUAUAUCCGAUUUCCUCGCAAAUAAGCAUAAAAAAAUACGUAUUAAUUACAGCUUUUAGAGUAACUUUAGGCCAUUAAAUAUAUUAUAAGCUAUAACCAC ..(((((.........((((.(((..(((((...((((.....)))))))))...))).)))).............(((((((.....(((((.......................)))))......))))))))))))((((((((((((......))))...(((...........(((((..((((.(((((((((((...........((((((.((.........(((((((.((......)))))))))..)))))))).....((((.......(((...(((((((....(.....).((((((......)))))).......))))))).)))....(((((((.(((.(((..((.(((((((((.((((.((((((........)))))).)))).))))))))).))..))).)))))))))).((((((.(((((.((((((((((...)))))))).))............((....))................))))))))))).............))))...)))))....)))))).........((......))............(((.....)))..))))..)))))......)))...........))))))))......... (-154.23) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 946 140 91 231 182 NC_002950:PG2020|5end_CRISPR-associated_TM1814_family_protein_2112273..2112503_NEGATIVE 772 290 241 109 60 NC_002950:PG1980|5end_hypothetical_protein_2067930..2068160_NEGATIVE 682 307 261 89 44 NC_002950:PG2064|5end_hypothetical_protein_2160550..2160780_POSITIVE 624 309 261 91 46 NC_002950:PG0313|5end_hypothetical_protein_347366..347596_POSITIVE 600 307 261 103 58 NC_002950:PG1799|5end_hypothetical_protein_1894499..1894729_POSITIVE 533 290 244 204 156 NC_002950:PG0081|5end_hypothetical_protein_93805..94035_POSITIVE 522 308 261 124 80 NC_002950:PG0174|5end_pyridine_nucleotide-disulphide_oxidoreductase_family_protein_197628..197858_NEGATIVE 502 308 265 157 113 NC_002950:PG1645|5end_ISPg5,_transposase_Orf1_1726062..1726292_NEGATIVE 494 290 242 131 83 NC_002950:PG0007|5end_hypothetical_protein_6913..7143_NEGATIVE 475 309 261 128 78 NC_002950:PG1617|5end_hypothetical_protein_1697755..1697985_NEGATIVE 412 257 211 231 186 NC_002950:PG0470|5end_hypothetical_protein_511883..512113_NEGATIVE 389 199 151 231 179 NC_002950:PG0486|5end_methylated-DNA--protein-cysteine_S-methyltransferase_525934..526164_NEGATIVE 380 499 457 200 145 NC_002950:PG1999|5end_hypothetical_protein_2088821..2089051_NEGATIVE 379 325 289 77 38 NC_002950:PG1313|5end_dipeptidase-related_protein_1392282..1392512_POSITIVE 377 310 261 194 138 NC_002950:PG0132|5end_hypothetical_protein_152879..153109_POSITIVE 372 326 282 204 162 NC_002950:PG1875|5end_hemolysin_1970621..1970851_NEGATIVE 372 307 269 49 1 NC_002950:PG0524|5end_hypothetical_protein_568599..568829_POSITIVE 371 640 592 100 53 NC_002950:PG1831|5end_ATP-dependent_DNA_helicase_RecQ_1925422..1925652_NEGATIVE 369 330 287 58 7 NC_002950:PG1887|5end_rhodanese-like_domain_protein_1984565..1984795_POSITIVE 360 290 241 158 109 NC_002950:PG2063|5end_hypothetical_protein_2160596..2160826_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 504 307 261 69 18 NC_002950:PG0542|3end_hypothetical_protein_595377..595527_NEGATIVE 377 280 232 145 97 NC_002950:PG1718|3end_hypothetical_protein_1806532..1806682_NEGATIVE 374 309 264 101 56 NC_002950:PG0732|3end_hypothetical_protein_783018..783168_POSITIVE 371 309 261 60 8 NC_002950:PG0155|3end_riboflavin_biosynthesis_protein_RibD_177861..178011_NEGATIVE 369 164 125 66 18 NC_002950:PG1608|3end_methylmalonyl-CoA_decarboxylase,_beta_subunit_1688685..1688835_NEGATIVE 364 274 234 36 1 NC_002950:PG1626|3end_hypothetical_protein_1710486..1710636_POSITIVE 361 327 287 52 4 NC_002950:PG0046|3end_phosphatidate_cytidylyltransferase_56346..56496_NEGATIVE 359 350 306 51 4 NC_002950:PG0423|3end_hypothetical_protein_462573..462723_POSITIVE 357 168 122 72 4 NC_002950:PG1556|3end_hypothetical_protein_1637001..1637151_POSITIVE 353 120 71 90 27 NC_002950:PG1389|3end_DNA-binding_protein,_histone-like_family_1470870..1471020_NEGATIVE 348 500 455 149 99 NC_002950:PG0333|3end_hypothetical_protein_368811..368961_POSITIVE 347 168 126 109 60 NC_002950:PG0164|3end_hypothetical_protein_187081..187231_NEGATIVE 345 360 315 120 60 NC_002950:PG1874|3end_hypothetical_protein_1969253..1969403_NEGATIVE 340 330 281 59 6 NC_002950:PG0325|3end_hypothetical_protein_359085..359235_NEGATIVE 338 167 122 91 49 NC_002950:PG0130|3end_phosphoglyceromutase_151922..152072_NEGATIVE 336 485 441 110 55 NC_002950:PG1769|3end_hypothetical_protein_1857010..1857160_POSITIVE 332 350 301 82 15 NC_002950:PG1695|3end_hypothetical_protein_1779711..1779861_POSITIVE 330 499 456 68 21 NC_002950:PG1954|3end_NAD_dependent_epimerase/reductase-related_protein_2045612..2045762_POSITIVE 330 497 460 77 42 NC_002950:PG1509|3end_HAD-superfamily_hydrolase,_subfamily_IA,_variant_1_family_protein_1587439..1587589_POSITIVE 330 135 91 75 31 NC_002950:PG1375|3end_hypothetical_protein_1456743..1456893_POSITIVE