Origin IGS: gagacatcccggttccttccgccgaccgtatcggatgttatcgctatccgctcaatccaatcctccgaggtacttcctacatcctgaccttaaacataacgtcagttcgacgtaatgttacagtccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaactttttccctttcattttctgattcggttatagtcaatatcccatcaagagagaatcggcaccatatcgcttacattgaactcacgttaaacatacgaagccatgtacacaatgtagttgtacatggcttcgaatccgtttatataaggcctaaaatccttccgac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gtcggaaggattttaggccttatataaacggattcgaagccatgtacaactacattgtgtacatggcttcgtatgtttaacgtgagttcaatgtaagcgatatggtgccgattctctcttgatgggatattgactataaccgaatcagaaaatgaaagggaaaaagtttgacgtctcatcggacgtcggagcctctcgaagctgaaacctctcaacagacaaatgaaatacattgacaaacagcatattgcatgtttttcttggatcgaatccgcattgttttatagccgttcggaacatacgccgaaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagagcgtacctcgccgagctctacagcgtagctcgaagagccgtacgggatagctcgtcatcctctatggtgtaccgccatctgccctatggtatagcccatctttctgtacggactgtaacattacgtcgaactgacgttatgtttaaggtcaggatgtaggaagtacctcggaggattggattgagcggatagcgataacatccgatacggtcggcggaaggaaccgggatgtctc Mask Tandem Repeat Region ================================================ gagacatcccggttccttccgccgaccgtatcggatgttatcgctatccgctcaatccaatcctccgaggtacttcctacatcctgaccttaaacataacgtcagttcgacgtaatgttacagtccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaactttttccctttcattttctgattcggttatagtcaatatcccatcaagagagaatcggcaccatatcgcttacattgaactcacgttaaacatacgaagccatgtacacaatgtagttgtacatggcttcgaatccgtttatataaggcctaaaatccttccgac Find is-nt database================================================ Query_seq: PG1954:PG1956|PG1954:PG1956:NAD dependent epimerase/reductase-related protein:4-hydroxybutyrate CoA-transferase:-><-:2045673..2046304 632 gagacatcccggttccttccgccgaccgtatcggatgttatcgctatccgctcaatccaatcctccgaggtacttcctacatcctgaccttaaacataacgtcagttcgacgtaatgttacagtccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaactttttccctttcattttctgattcggttatagtcaatatcccatcaagagagaatcggcaccatatcgcttacattgaactcacgttaaacatacgaagccatgtacacaatgtagttgtacatggcttcgaatccgtttatataaggcctaaaatccttccgac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1954:PG1956|PG1954:PG1956:NAD dependent epimerase/reductase-related protein:4-hydroxybutyrate CoA-transferase:-><-:2045673..2046304 632 gagacatcccggttccttccgccgaccgtatcggatgttatcgctatccgctcaatccaatcctccgaggtacttcctacatcctgaccttaaacataacgtcagttcgacgtaatgttacagtccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaactttttccctttcattttctgattcggttatagtcaatatcccatcaagagagaatcggcaccatatcgcttacattgaactcacgttaaacatacgaagccatgtacacaatgtagttgtacatggcttcgaatccgtttatataaggcctaaaatccttccgac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1954:PG1956|PG1954:PG1956:NAD dependent epimerase/reductase-related protein:4-hydroxybutyrate CoA-transferase:-><-:2045673..2046304 632 gagacatcccggttccttccgccgaccgtatcggatgttatcgctatccgctcaatccaatcctccgaggtacttcctacatcctgaccttaaacataacgtcagttcgacgtaatgttacagtccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaactttttccctttcattttctgattcggttatagtcaatatcccatcaagagagaatcggcaccatatcgcttacattgaactcacgttaaacatacgaagccatgtacacaatgtagttgtacatggcttcgaatccgtttatataaggcctaaaatccttccgac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gagacatcccggttccttccgccgaccgtatcggatgttatcgctatccgctcaatccaatcctccgaggtacttcctacatcctgaccttaaacataacgtcagttcgacgtaatgttacagtccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaactttttccctttcattttctgattcggttatagtcaatatcccatcaagagagaatcggcaccatatcgcttacattgaactcacgttaaacatacgaagccatgtacacaatgtagttgtacatggcttcgaatccgtttatataaggcctaaaatccttccgac Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: + Start: 6 End: 149 ..... M P V P S A D R I G C Y R Y P L N P I L R G T S Y I L T L N I T S V R R N V T V R T E R W A I P ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 37 Strand: + Start: 178 End: 288 ................................................................................................................................................................................. M T S Y P V R L F E L R C R A R R G T L Y S V A R R A V E R S S V R S T A ........................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 51 Strand: + Start: 333 End: 485 ............................................................................................................................................................................................................................................................................................................................................ M F R T A I K Q C G F D P R K T C N M L F V N V F H L S V E R F Q L R E A P T S D E T S N F F P F I F ................................................................................................................................................... Protein_Len: 60 Strand: + Start: 367 End: 630 .............................................................................................................................................................................................................................................................................................................................................................................. M Q E K H A I C C L S M Y F I C L L R G F S F E R L R R P M R R Q T F S L S F S D S V I V N I P S R E N R H H I A Y I E .. Protein_Len: 39 Strand: - Start: 284 End: 400 ........................................................................................................................................................................................................................................................................................... P T A R T A T P R L E V G K R R I N R V A I F C H P N S G L F V H L I S N T M ........................................................................................................................................................................................................................................ Protein_Len: 60 Strand: - Start: 240 End: 455 ............................................................................................................................................................................................................................................... R E V A Y G S D S Y P T A R G G K P T H E S R S Y F L A S E I W S F C A I H Q K D I Y K M Q R N L P K L K S L S R R G M ................................................................................................................................................................................. Protein_Len: 31 Strand: - Start: 80 End: 172 ............................................................................... M R V K F M V D T R R L T V T R V S L H A I G Y P L H R Y V M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................ gagacatcccggttccttccgccgaccgtatcggatgttatcgctatccgctcaatccaatcctccgaggtacttcctacatcctgaccttaaacataacgtcagttcgacgtaatgttacagtccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaactttttccctttcattttctgattcggttatagtcaatatcccatcaagagagaatcggcaccatatcgcttacattgaactcacgttaaacatacgaagccatgtacacaatgtagttgtacatggcttcgaatccgtttatataaggcctaaaatccttccgac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gagacatcccggttccttccgccgaccgtatcggatgttatcgctatccgctcaatccaatcctccgaggtacttcctacatcctgaccttaaacataacgtcagttcgacgtaatgttacagtccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaactttttccctttcattttctgattcggttatagtcaatatcccatcaagagagaatcggcaccatatcgcttacattgaactcacgttaaacatacgaagccatgtacacaatgtagttgtacatggcttcgaatccgtttatataaggcctaaaatccttccgac Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -16.7 Tail_Score: -3.04263 Start: 256 End: 287 Full_Region: agcgactcgggctac gccgtagagcgta ccgagc tgcgctctacggc tcttcgagctacgct ...............................................................................................................................................................................................................................................................gccgtagagcgtaccgagctgcgctctacggc......................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 54 HP_score: -6.7 Tail_Score: -3.01905 Start: 557 End: 603 Full_Region: TTGAACTCACGTTAA ACATACGAAGCCATGTACA CAATGTAGT TGTACATGGCTTCGAATCC GTTTATATAAGGCCT ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ACATACGAAGCCATGTACACAATGTAGTTGTACATGGCTTCGAATCC............................. TransTerm Strand: + Conf: 59 HP_score: -4.0 Tail_Score: -4.0371 Start: 558 End: 604 Full_Region: TGAACTCACGTTAAA CATACGAAGCCATGTACA CAATGTAGT TGTACATGGCTTCGAATCCG TTTATATAAGGCCTA .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CATACGAAGCCATGTACACAATGTAGTTGTACATGGCTTCGAATCCG............................ TransTerm Strand: - Conf: 77 HP_score: -10.2 Tail_Score: -3.6215 Start: 558 End: 602 Full_Region: ggccttatataaacg gattcgaagccatgtaca actacattg tgtacatggcttcgtatg tttaacgtgagttca .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gattcgaagccatgtacaactacattgtgtacatggcttcgtatg.............................. TransTerm Strand: - Conf: 82 HP_score: -12.8 Tail_Score: -3.3879 Start: 560 End: 600 Full_Region: ccttatataaacgga ttcgaagccatgtaca actacattg tgtacatggcttcgta tgtttaacgtgagtt ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttcgaagccatgtacaactacattgtgtacatggcttcgta................................ TransTerm Strand: - Conf: 90 HP_score: -15.4 Tail_Score: -3.26031 Start: 562 End: 598 Full_Region: ttatataaacggatt cgaagccatgtaca actacattg tgtacatggcttcg tatgtttaacgtgag .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cgaagccatgtacaactacattgtgtacatggcttcg.................................. Find igs database================================================ Query_seq: PG1954:PG1956|PG1954:PG1956:NAD dependent epimerase/reductase-related protein:4-hydroxybutyrate CoA-transferase:-><-:2045673..2046304 632 gagacatcccggttccttccgccgaccgtatcggatgttatcgctatccgctcaatccaatcctccgaggtacttcctacatcctgaccttaaacataacgtcagttcgacgtaatgttacagtccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaactttttccctttcattttctgattcggttatagtcaatatcccatcaagagagaatcggcaccatatcgcttacattgaactcacgttaaacatacgaagccatgtacacaatgtagttgtacatggcttcgaatccgtttatataaggcctaaaatccttccgac Intra-Species Hit: Count: 7 Min: 1 Max: 632 Len: 632 Subject: NC_002950_PG1954_PG1956|NAD dependent epimerase/reductase-related protein:4-hydroxybutyrate CoA-transferase|POSITIVE:NEGATIVE|[2045673,2046304]|632 HSP 1 e-value: 0.0 bit: 1253.0 Len: 632 Query Start:1 Query End:632 Subject Strand: POSITIVE Subject Start: 1 Subject End: 632 gagacatcccggttccttccgccgaccgtatcggatgttatcgctatccgctcaatccaatcctccgaggtacttcctacatcctgaccttaaacataacgtcagttcgacgtaatgttacagtccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaactttttccctttcattttctgattcggttatagtcaatatcccatcaagagagaatcggcaccatatcgcttacattgaactcacgttaaacatacgaagccatgtacacaatgtagttgtacatggcttcgaatccgtttatataaggcctaaaatccttccgac gagacatcccggttccttccgccgaccgtatcggatgttatcgctatccgctcaatccaatcctccgaggtacttcctacatcctgaccttaaacataacgtcagttcgacgtaatgttacagtccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaactttttccctttcattttctgattcggttatagtcaatatcccatcaagagagaatcggcaccatatcgcttacattgaactcacgttaaacatacgaagccatgtacacaatgtagttgtacatggcttcgaatccgtttatataaggcctaaaatccttccgac HSP 2 e-value: 2.0E-6 bit: 56.0 Len: 28 Query Start:234 Query End:261 Subject Strand: NEGATIVE Subject Start: 194 Subject End: 221 .........................................................................................................................................................................................................................................gctctacagcgtagctcgaagagccgta................................................................................................................................................................................................................................................................................................................................................................................... .........................................................................................................................................................................................................................................gctctacagcgtagctcgaagagccgta................................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 2.0E-6 bit: 56.0 Len: 28 Query Start:194 Query End:221 Subject Strand: NEGATIVE Subject Start: 234 Subject End: 261 .................................................................................................................................................................................................tacggctcttcgagctacgctgtagagc........................................................................................................................................................................................................................................................................................................................................................................................................................... .................................................................................................................................................................................................tacggctcttcgagctacgctgtagagc........................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_002950_PG0890_PG0893|alkaline phosphatase, putative:hydroxylamine reductase|POSITIVE:NEGATIVE|[953164,954290]|1127 HSP 1 e-value: 1.0E-134 bit: 480.0 Len: 318 Query Start:237 Query End:554 Subject Strand: POSITIVE Subject Start: 568 Subject End: 885 ............................................................................................................................................................................................................................................ctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaactttttccctttcattttctgattcggttatagtcaatatcccatcaagagagaatcggcaccatatcgcttacattgaactcacgtt.............................................................................. ............................................................................................................................................................................................................................................ctacagcgtagcttgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaatggctataaaacaatgcggattcgatctgaagaaaatatgcaatatgctgtttgtcagcgtatttcatttctcggttgagaaatttcagcttcgagaggcttcgacatccgatgagacgtcaaactttttccctttcattttctgattcgattatagtcaatatcccaacaagagagagtcggcaccatatcgcttacatcgaactcacgtt.............................................................................. HSP 2 e-value: 1.0E-41 bit: 172.0 Len: 135 Query Start:114 Query End:248 Subject Strand: POSITIVE Subject Start: 357 Subject End: 491 .................................................................................................................aatgttacagtccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagc................................................................................................................................................................................................................................................................................................................................................................................................ .................................................................................................................aatgttgcagtccgcaccggaagatgggctataccacaaggcagatggcggtattccgtagaggataacgggctatcccatacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagc................................................................................................................................................................................................................................................................................................................................................................................................ HSP 3 e-value: 2.0E-6 bit: 56.0 Len: 28 Query Start:234 Query End:261 Subject Strand: NEGATIVE Subject Start: 437 Subject End: 464 .........................................................................................................................................................................................................................................gctctacagcgtagctcgaagagccgta................................................................................................................................................................................................................................................................................................................................................................................... .........................................................................................................................................................................................................................................gctctacagcgtagctcgaagagccgta................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_002950_PG1881_PG1884|hypothetical protein:alpha-L-fucosidase precursor, putative|POSITIVE:NEGATIVE|[1978107,1979057]|951 HSP 1 e-value: 1.0E-118 bit: 428.0 Len: 264 Query Start:237 Query End:500 Subject Strand: POSITIVE Subject Start: 567 Subject End: 829 ............................................................................................................................................................................................................................................ctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaactttttccctttcattttctgattcggttatagt.................................................................................................................................... ............................................................................................................................................................................................................................................ctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggagtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaacgtatttcatttctcggttgagagatttcagcttcgggagacttcgacatccgatgagacgtcaaattttttccctttcattttctga-tcggttatagt.................................................................................................................................... HSP 2 e-value: 3.0E-51 bit: 204.0 Len: 135 Query Start:114 Query End:248 Subject Strand: POSITIVE Subject Start: 356 Subject End: 490 .................................................................................................................aatgttacagtccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagc................................................................................................................................................................................................................................................................................................................................................................................................ .................................................................................................................aatgttacagtccgtacagaaagatgggctataccatagggcacatggaggtactccgtaggggatgacgagctatcccgtacagctcttcgagctacgctgtagagctcagcgaggtacgccctacagcgtagc................................................................................................................................................................................................................................................................................................................................................................................................ HSP 3 e-value: 1.0E-7 bit: 60.0 Len: 34 Query Start:194 Query End:227 Subject Strand: NEGATIVE Subject Start: 558 Subject End: 591 .................................................................................................................................................................................................tacggctcttcgagctacgctgtagagctcggcg..................................................................................................................................................................................................................................................................................................................................................................................................................... .................................................................................................................................................................................................tacggctcttcgagctacgctgtagggctcggcg..................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_002950_PG1190_PG1195|glycerate dehydrogenase:8-amino-7-oxononanoate synthase|POSITIVE:POSITIVE|[1273206,1274118]|913 HSP 1 e-value: 1.0E-107 bit: 391.0 Len: 281 Query Start:237 Query End:517 Subject Strand: NEGATIVE Subject Start: 139 Subject End: 418 ............................................................................................................................................................................................................................................ctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaactttttccctttcattttctgattcggttatagtcaatatcccatcaagag................................................................................................................... ............................................................................................................................................................................................................................................ctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccccttcggcatatgttccgaacggctataaaacaatgcggattcaatccaggaaaaatatgcaatatgctgtttatcagcatatttcatttctcagttgaaagatttcagcttcgagaggcttcgacatccgatgagacttcaaactttttccctttcattttctga-tcggttatagtcaatgccccaacaagag................................................................................................................... HSP 2 e-value: 3.0E-51 bit: 204.0 Len: 135 Query Start:114 Query End:248 Subject Strand: NEGATIVE Subject Start: 495 Subject End: 629 .................................................................................................................aatgttacagtccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagc................................................................................................................................................................................................................................................................................................................................................................................................ .................................................................................................................aatgttgcagtccgtatagggagatgggctataccacagggcagatggcggtactccgtagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgccctacagcgtagc................................................................................................................................................................................................................................................................................................................................................................................................ HSP 3 e-value: 2.0E-6 bit: 56.0 Len: 28 Query Start:234 Query End:261 Subject Strand: POSITIVE Subject Start: 522 Subject End: 549 .........................................................................................................................................................................................................................................gctctacagcgtagctcgaagagccgta................................................................................................................................................................................................................................................................................................................................................................................... .........................................................................................................................................................................................................................................gctctacagcgtagctcgaagagccgta................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_002950_PG0237_PG0240|uracil-DNA glycosylase:hydrolase, haloacid dehalogenase-like family|POSITIVE:NEGATIVE|[275587,276484]|898 HSP 1 e-value: 1.0E-104 bit: 381.0 Len: 264 Query Start:254 Query End:517 Subject Strand: POSITIVE Subject Start: 448 Subject End: 711 .............................................................................................................................................................................................................................................................gagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatccaagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaactttttccctttcattttctgattcggttatagtcaatatcccatcaagag................................................................................................................... .............................................................................................................................................................................................................................................................gagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaatggctataaaacaatgcggattcgatctgaagaaaatatgcaatatcctgtttgtcagcgtatttcatttctcggttgagagatttcagcttcgagaggcttcgacatccaatgagacgtcaaactttttccctttcattttctgattcggttatagtcaatgccccaacaagag................................................................................................................... HSP 2 e-value: 3.0E-51 bit: 204.0 Len: 135 Query Start:114 Query End:248 Subject Strand: POSITIVE Subject Start: 264 Subject End: 398 .................................................................................................................aatgttacagtccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagc................................................................................................................................................................................................................................................................................................................................................................................................ .................................................................................................................aatgttgcagtccgtacaggaagatgggctataccacaaggcagatggcggtacaccgtagaggataacgggctatcccgtacagctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagc................................................................................................................................................................................................................................................................................................................................................................................................ HSP 3 e-value: 4.0E-7 bit: 58.0 Len: 41 Query Start:517 Query End:557 Subject Strand: POSITIVE Subject Start: 736 Subject End: 776 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gagaatcggcaccatatcgcttacattgaactcacgttaaa........................................................................... ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gagaatcgacaccatatcgcttacatcgacctcacgttaaa........................................................................... Subject: NC_002950_PG1297_PG1300|ribosomal protein S1:hypothetical protein|POSITIVE:NEGATIVE|[1377771,1378619]|849 HSP 1 e-value: 4.0E-90 bit: 333.0 Len: 231 Query Start:237 Query End:467 Subject Strand: POSITIVE Subject Start: 504 Subject End: 735 ............................................................................................................................................................................................................................................ctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgcggattcgatc-caagaaaaacatgcaatatgctgtttgtcaatgtatttcatttgtctgttgagaggtttcagcttcgagaggctccgacgtccgatgagacgtcaaac..................................................................................................................................................................... ............................................................................................................................................................................................................................................ctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccccttcggagtatattccgaacggctataaaacaatgaggattcgatctgaagaaaaacatgcaatatgctgtttgtcagcgtatttcacttctcagttcaaagatttcagcttcgagaggctccgacatccgatgagacgtcaaac..................................................................................................................................................................... HSP 2 e-value: 4.0E-35 bit: 151.0 Len: 135 Query Start:114 Query End:248 Subject Strand: POSITIVE Subject Start: 292 Subject End: 427 .................................................................................................................aatgttacagtccgtacagaaagatgggctataccatagggca-gatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagc................................................................................................................................................................................................................................................................................................................................................................................................ .................................................................................................................aatgttgcagtccgtacagggagatgggctataccacagggcaagatggcggtactccatagaggataacgggctatcccgtacagctcttcgagctacgctgtagagcccggcgcggtgagccctacagcgtagc................................................................................................................................................................................................................................................................................................................................................................................................ HSP 3 e-value: 6.0E-6 bit: 54.0 Len: 31 Query Start:194 Query End:224 Subject Strand: NEGATIVE Subject Start: 498 Subject End: 528 .................................................................................................................................................................................................tacggctcttcgagctacgctgtagagctcg........................................................................................................................................................................................................................................................................................................................................................................................................................ .................................................................................................................................................................................................tacggctcttcgagctacgctgtagggctcg........................................................................................................................................................................................................................................................................................................................................................................................................................ Subject: NC_002950_PG1837_PG1840|hemagglutinin protein HagA:hypothetical protein|POSITIVE:POSITIVE|[1936795,1937241]|447 HSP 1 e-value: 3.0E-57 bit: 224.0 Len: 145 Query Start:215 Query End:359 Subject Strand: NEGATIVE Subject Start: 107 Subject End: 251 ......................................................................................................................................................................................................................gtagagctcggcgaggtacgctctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgaccccctttcggcgtatgttccgaacggctataaaacaatgc................................................................................................................................................................................................................................................................................. ......................................................................................................................................................................................................................gtagagcttggcgcggtgagccctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgacccccttccggagtatattccgaacggctataaaacaatgc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-43 bit: 176.0 Len: 125 Query Start:124 Query End:248 Subject Strand: NEGATIVE Subject Start: 306 Subject End: 430 ...........................................................................................................................tccgtacagaaagatgggctataccatagggcagatggcggtacaccatagaggatgacgagctatcccgtacggctcttcgagctacgctgtagagctcggcgaggtacgctctacagcgtagc................................................................................................................................................................................................................................................................................................................................................................................................ ...........................................................................................................................tccgaacaggaagatgggctataccacagggcagatggtggtactccgtagaggataacgggctatcccgtacggctcttcgagctacgctgtagagctcagcgaggtacgctctacagcgtagc................................................................................................................................................................................................................................................................................................................................................................................................ HSP 3 e-value: 2.0E-6 bit: 56.0 Len: 28 Query Start:234 Query End:261 Subject Strand: POSITIVE Subject Start: 333 Subject End: 360 .........................................................................................................................................................................................................................................gctctacagcgtagctcgaagagccgta................................................................................................................................................................................................................................................................................................................................................................................... .........................................................................................................................................................................................................................................gctctacagcgtagctcgaagagccgta................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAGACAUCCCGGUUCCUUCCGCCGACCGUAUCGGAUGUUAUCGCUAUCCGCUCAAUCCAAUCCUCCGAGGUACUUCCUACAUCCUGACCUUAAACAUAACGUCAGUUCGACGUAAUGUUACAGUCCGUACAGAAAGAUGGGCUAUACCAUAGGGCAGAUGGCGGUACACCAUAGAGGAUGACGAGCUAUCCCGUACGGCUCUUCGAGCUACGCUGUAGAGCUCGGCGAGGUACGCUCUACAGCGUAGCUCGAAGAGCCGUAGAGCGCAGCUCGGUACGCUCUACGGCGUAGCCCGAGUCGCUGUAGGGCGUAGCUCGACCCCCUUUCGGCGUAUGUUCCGAACGGCUAUAAAACAAUGCGGAUUCGAUCCAAGAAAAACAUGCAAUAUGCUGUUUGUCAAUGUAUUUCAUUUGUCUGUUGAGAGGUUUCAGCUUCGAGAGGCUCCGACGUCCGAUGAGACGUCAAACUUUUUCCCUUUCAUUUUCUGAUUCGGUUAUAGUCAAUAUCCCAUCAAGAGAGAAUCGGCACCAUAUCGCUUACAUUGAACUCACGUUAAACAUACGAAGCCAUGUACACAAUGUAGUUGUACAUGGCUUCGAAUCCGUUUAUAUAAGGCCUAAAAUCCUUCCGAC .........(((..(....((((((.......((.(((.(((((((((.((((.............(((((.(............)))))).(((((.(((((.....))))).)))))..(((((((........)))))))........)))).))))))))))))))...((((..(.(((((((((((.((((((((((((((((((((((((((((...((...))..)))))))))))))))))))))))))))).((((..((((((((((((....))))))..))))))))))...))).)))))))).)..))))))))))...)..)))...((((.((.......((((((((.......((.(((((.((.....))))))).((((((((.......((((...(((((((....(((((....)))))..((((((......))))))..........)))))))(((((((((..((.(((.....))).)).))).))))))....))))..........))))))))..)).............((((((((((((((......).))))))))))))))))))))).....)).))))............... (-236.63) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUCGGAAGGAUUUUAGGCCUUAUAUAAACGGAUUCGAAGCCAUGUACAACUACAUUGUGUACAUGGCUUCGUAUGUUUAACGUGAGUUCAAUGUAAGCGAUAUGGUGCCGAUUCUCUCUUGAUGGGAUAUUGACUAUAACCGAAUCAGAAAAUGAAAGGGAAAAAGUUUGACGUCUCAUCGGACGUCGGAGCCUCUCGAAGCUGAAACCUCUCAACAGACAAAUGAAAUACAUUGACAAACAGCAUAUUGCAUGUUUUUCUUGGAUCGAAUCCGCAUUGUUUUAUAGCCGUUCGGAACAUACGCCGAAAGGGGGUCGAGCUACGCCCUACAGCGACUCGGGCUACGCCGUAGAGCGUACCGAGCUGCGCUCUACGGCUCUUCGAGCUACGCUGUAGAGCGUACCUCGCCGAGCUCUACAGCGUAGCUCGAAGAGCCGUACGGGAUAGCUCGUCAUCCUCUAUGGUGUACCGCCAUCUGCCCUAUGGUAUAGCCCAUCUUUCUGUACGGACUGUAACAUUACGUCGAACUGACGUUAUGUUUAAGGUCAGGAUGUAGGAAGUACCUCGGAGGAUUGGAUUGAGCGGAUAGCGAUAACAUCCGAUACGGUCGGCGGAAGGAACCGGGAUGUCUC ((.(..(((.((((((..(((((((.((((....((((((((((((((.(......)))))))))))))))..)))).(((....)))..)))))))(((...(((.(((((..(((...((((((((...((((....((...(((.....)))..)).....))))....))))))))))).))))).)))..)))...)))))))))..).)).((((................(((((((.....)).))))).((((((.((...(((((...........((((((((((...(((((((..((((((((((((((..(((...((((.(((((..(((((......))))))))))...)))).((((((((((((((((((((((((((((............)))))))))))))))))))))))))))).))).)))))))..))))))).))))))).(((.(((((((((..(((((..((((((((........((((..(((((.(((((.....))))).)))))...))))))))))..))..)))))....)))..........)))))).)))......))))).)))))..)))))..)).)))))))))).. (-253.42) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1109 240 191 225 176 NC_002950:PG1840|5end_hypothetical_protein_1937102..1937332_POSITIVE 509 288 243 212 152 NC_002950:PG0854|5end_hypothetical_protein_914870..915100_POSITIVE 496 328 285 68 20 NC_002950:PG1992|5end_glucose-inhibited_division_protein_A_2081410..2081640_POSITIVE 471 49 1 97 41 NC_002950:PG2069|5end_oxidoreductase,_short_chain_dehydrogenase/reductase_family_2164798..2165028_POSITIVE 471 329 284 208 147 NC_002950:PG0595|5end_30S_ribosomal_protein_S6_653008..653238_POSITIVE 456 310 264 192 144 NC_002950:PG2036|5end_ion_transporter_2136561..2136791_POSITIVE 454 317 271 75 27 NC_002950:PG1190|5end_glycerate_dehydrogenase_1272112..1272342_POSITIVE 453 230 183 56 4 NC_002950:PG1576|5end_L-aspartate_oxidase_1653016..1653246_POSITIVE 453 318 271 199 148 NC_002950:PG0356|5end_hypothetical_protein_390611..390841_POSITIVE 450 317 277 161 112 NC_002950:PG2087|5end_hypothetical_protein_2186990..2187220_POSITIVE 450 258 212 56 13 NC_002950:PG1118|5end_clpB_protein_1194674..1194904_NEGATIVE 438 303 263 211 161 NC_002950:PG2074|5end_hypothetical_protein_2174661..2174891_NEGATIVE 438 303 263 211 161 NC_002950:PG1670|5end_hypothetical_protein_1755853..1756083_NEGATIVE 438 303 263 211 161 NC_002950:PG1265|5end_hypothetical_protein_1345182..1345412_NEGATIVE 438 303 263 211 161 NC_002950:PG0102|5end_hypothetical_protein_121003..121233_POSITIVE 429 230 185 51 2 NC_002950:PG0223|5end_exonuclease_257610..257840_NEGATIVE 428 310 261 197 143 NC_002950:PG2072|5end_UvrD/REP_helicase_domain_protein_2167369..2167599_POSITIVE 428 310 263 205 146 NC_002950:PG1943|5end_hypothetical_protein_2030260..2030490_NEGATIVE 426 308 266 56 9 NC_002950:PG1752|5end_hypothetical_protein_1838394..1838624_POSITIVE 424 309 264 225 178 NC_002950:PG1352|5end_hypothetical_protein_1433150..1433380_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 657 554 515 87 50 NC_002950:PG1175|3end_ABC_transporter,_ATP-binding_protein,_putative_1254170..1254320_NEGATIVE 547 217 174 52 8 NC_002950:PG0261|3end_ISPg3,_transposase_293862..294012_NEGATIVE 541 555 515 41 3 NC_002950:PG1884|3end_alpha-L-fucosidase_precursor,_putative_1978968..1979118_NEGATIVE 480 300 256 104 51 NC_002950:PG1012|3end_tRNA-i(6)A37_modification_enzyme_MiaB_1070089..1070239_NEGATIVE 471 49 1 91 35 NC_002950:PG2068|3end_glycerol-3-phosphate_cytidylyltransferase_2164872..2165022_POSITIVE 471 329 284 111 50 NC_002950:PG0594|3end_RNA_polymerase_sigma-70_factor_652991..653141_POSITIVE 471 269 221 85 30 NC_002950:PG0085|3end_alpha-galactosidase_100556..100706_POSITIVE 455 456 413 143 76 NC_002950:PG1151|3end_alcohol_dehydrogenase,_iron-containing_1234378..1234528_POSITIVE 438 300 256 138 85 NC_002950:PG0569|3end_hypothetical_protein_624697..624847_NEGATIVE 435 327 284 67 23 NC_002950:PG0138|3end_malonyl_CoA-acyl_carrier_protein_transacylase_161634..161784_POSITIVE 428 309 266 131 79 NC_002950:PG0381|3end_cell_volume_regulation_protein_CvrA_411185..411335_NEGATIVE 426 308 266 72 25 NC_002950:PG1751|3end_aminotransferase,_class_V_1838490..1838640_POSITIVE 420 309 266 133 82 NC_002950:PG1104|3end_hypothetical_protein_1175782..1175932_POSITIVE 415 287 241 74 28 NC_002950:PG0459|3end_ISPg5,_transposase_Orf1_501517..501667_NEGATIVE 413 228 182 87 30 NC_002950:PG0032|3end_beta-mannosidase,_putative_37627..37777_NEGATIVE 411 240 191 128 79 NC_002950:PG1675|3end_hypothetical_protein_1760360..1760510_POSITIVE 410 463 424 75 28 NC_002950:PG2102|3end_immunoreactive_61_kDa_antigen_PG91_2211424..2211574_NEGATIVE 402 230 182 142 82 NC_002950:PG0629|3end_inorganic_polyphosphate/ATP-NAD_kinase_682498..682648_NEGATIVE 401 297 252 88 38 NC_002950:PG0328|3end_imidazolonepropionase_362155..362305_NEGATIVE 401 237 192 60 5 NC_002950:PG0224|3end_hypothetical_protein_257614..257764_NEGATIVE