Origin IGS: tcacagcctgcctccttttatcgcaagacaaaggctctgccattgacttcgctttcttgctttactaaagcttggcagacagaagctgcttgttcctttcttaggggatgcagagaggaagtttatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aataaacttcctctctgcatcccctaagaaaggaacaagcagcttctgtctgccaagctttagtaaagcaagaaagcgaagtcaatggcagagcctttgtcttgcgataaaaggaggcaggctgtga Mask Tandem Repeat Region ================================================ tcacagcctgcctccttttatcgcaagacaaaggctctgccattgacttcgctttcttgctttactaaagcttggcagacagaagctgcttgttcctttcttaggggatgcagagaggaagtttatt Find is-nt database================================================ Query_seq: PG1896:PG1897|PG1896:PG1897:S-adenosylmethionine synthetase:thiamin pyrophosphokinase catalytic domain protein:-><-:1996016..1996142 127 tcacagcctgcctccttttatcgcaagacaaaggctctgccattgacttcgctttcttgctttactaaagcttggcagacagaagctgcttgttcctttcttaggggatgcagagaggaagtttatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1896:PG1897|PG1896:PG1897:S-adenosylmethionine synthetase:thiamin pyrophosphokinase catalytic domain protein:-><-:1996016..1996142 127 tcacagcctgcctccttttatcgcaagacaaaggctctgccattgacttcgctttcttgctttactaaagcttggcagacagaagctgcttgttcctttcttaggggatgcagagaggaagtttatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1896:PG1897|PG1896:PG1897:S-adenosylmethionine synthetase:thiamin pyrophosphokinase catalytic domain protein:-><-:1996016..1996142 127 tcacagcctgcctccttttatcgcaagacaaaggctctgccattgacttcgctttcttgctttactaaagcttggcagacagaagctgcttgttcctttcttaggggatgcagagaggaagtttatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tcacagcctgcctccttttatcgcaagacaaaggctctgccattgacttcgctttcttgctttactaaagcttggcagacagaagctgcttgttcctttcttaggggatgcagagaggaagtttatt Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 37 End: 126 .................................... M P L T S L S C F T K A W Q T E A A C S F L R G C R E E V Y . Protein_Len: 31 Strand: - Start: 21 End: 113 .................... R L V F A R G N V E S E Q K V L A Q C V S A A Q E K R L P H M .............. Protein_Len: 42 Strand: - Start: 4 End: 126 ... * L R G G K I A L C L S Q W Q S R K R A K S F S P L C F S S T G K K P S A S L F N M . tcacagcctgcctccttttatcgcaagacaaaggctctgccattgacttcgctttcttgctttactaaagcttggcagacagaagctgcttgttcctttcttaggggatgcagagaggaagtttatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tcacagcctgcctccttttatcgcaagacaaaggctctgccattgacttcgctttcttgctttactaaagcttggcagacagaagctgcttgttcctttcttaggggatgcagagaggaagtttatt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG1896:PG1897|PG1896:PG1897:S-adenosylmethionine synthetase:thiamin pyrophosphokinase catalytic domain protein:-><-:1996016..1996142 127 tcacagcctgcctccttttatcgcaagacaaaggctctgccattgacttcgctttcttgctttactaaagcttggcagacagaagctgcttgttcctttcttaggggatgcagagaggaagtttatt Intra-Species Hit: Count: 1 Min: 1 Max: 127 Len: 127 Subject: NC_002950_PG1896_PG1897|S-adenosylmethionine synthetase:thiamin pyrophosphokinase catalytic domain protein|POSITIVE:NEGATIVE|[1996016,1996142]|127 HSP 1 e-value: 2.0E-66 bit: 252.0 Len: 127 Query Start:1 Query End:127 Subject Strand: POSITIVE Subject Start: 1 Subject End: 127 tcacagcctgcctccttttatcgcaagacaaaggctctgccattgacttcgctttcttgctttactaaagcttggcagacagaagctgcttgttcctttcttaggggatgcagagaggaagtttatt tcacagcctgcctccttttatcgcaagacaaaggctctgccattgacttcgctttcttgctttactaaagcttggcagacagaagctgcttgttcctttcttaggggatgcagagaggaagtttatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCACAGCCUGCCUCCUUUUAUCGCAAGACAAAGGCUCUGCCAUUGACUUCGCUUUCUUGCUUUACUAAAGCUUGGCAGACAGAAGCUGCUUGUUCCUUUCUUAGGGGAUGCAGAGAGGAAGUUUAUU ...(((...((((....((.((....)).)))))).))).....((((((.((((((.((.............(((((.(....)))))).(((((((....))))))))))))))))))))).... (-33.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAAACUUCCUCUCUGCAUCCCCUAAGAAAGGAACAAGCAGCUUCUGUCUGCCAAGCUUUAGUAAAGCAAGAAAGCGAAGUCAAUGGCAGAGCCUUUGUCUUGCGAUAAAAGGAGGCAGGCUGUGA .......(((((.(((..........))).)))))...(((((..((((((.((..((((.....))))......((.(((.(((.(((...))).))).))))).......))))))))))))).. (-37.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 357 118 71 54 2 NC_002950:PG0275|5end_thioredoxin_family_protein_307464..307694_NEGATIVE 355 102 62 113 69 NC_002950:PG1933|5end_ribosomal_protein_L22_2021967..2022197_NEGATIVE 340 79 33 204 162 NC_002950:PG1352|5end_hypothetical_protein_1433150..1433380_NEGATIVE 331 59 12 65 7 NC_002950:PG1728|5end_cytidine/deoxycytidylate_deaminase_family_protein_1817644..1817874_POSITIVE 329 110 68 221 174 NC_002950:PG1864|5end_leucine-rich_protein_1964309..1964539_NEGATIVE 324 79 33 169 119 NC_002950:PG1199|5end_hypothetical_protein_1276893..1277123_POSITIVE 323 74 33 99 50 NC_002950:PG2210|5end_excinuclease_ABC,_A_subunit_2323810..2324040_NEGATIVE 322 45 3 38 1 NC_002950:PG1861|5end_hypothetical_protein_1960057..1960287_NEGATIVE 321 119 74 169 121 NC_002950:PG0985|5end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_1046930..1047160_POSITIVE 318 75 31 198 157 NC_002950:PG0149|5end_hypothetical_protein_172203..172433_NEGATIVE 317 60 13 206 157 NC_002950:PG2016|5end_CRISPR-associated_helicase_Cas3_2108370..2108600_NEGATIVE 316 59 11 223 167 NC_002950:PG1498|5end_hypothetical_protein_1574656..1574886_POSITIVE 315 118 75 161 121 NC_002950:PG2218|5end_potassium_uptake_protein_TrkA_2331580..2331810_POSITIVE 315 42 4 209 157 NC_002950:PG2032|5end_primosomal_protein_N'_2133381..2133611_NEGATIVE 312 66 21 70 13 NC_002950:PG1963|5end_Sua5/YciO/YrdC/YwlC_family_protein_2052338..2052568_POSITIVE 312 120 74 160 102 NC_002950:PG1024|5end_hypothetical_protein_1086698..1086928_POSITIVE 310 119 71 59 7 NC_002950:PG0197|5end_hypothetical_protein_236204..236434_NEGATIVE 309 59 12 96 40 NC_002950:PG0081|5end_hypothetical_protein_93805..94035_POSITIVE 308 77 36 53 3 NC_002950:PG1925|5end_ribosomal_protein_S14_2018675..2018905_NEGATIVE 304 76 32 73 29 NC_002950:PG1195|5end_8-amino-7-oxononanoate_synthase_1273979..1274209_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 337 118 71 72 7 NC_002950:PG1620|3end_carboxyl-terminal_protease-related_protein_1699415..1699565_NEGATIVE 316 59 11 122 66 NC_002950:PG1497|3end_DNA-binding_protein,_histone-like_family_1574635..1574785_POSITIVE 315 118 75 108 68 NC_002950:PG2217|3end_1-deoxy-D-xylulose-5-phosphate_synthase_2331607..2331757_POSITIVE 308 77 36 51 1 NC_002950:PG1926|3end_50S_ribosomal_protein_L5_2018677..2018827_NEGATIVE 307 120 72 84 12 NC_002950:PG2219|3end_potassium_uptake_protein_TrkH_2334490..2334640_POSITIVE 305 46 5 128 75 NC_002950:PG2004|3end_hypothetical_protein_2093135..2093285_NEGATIVE 305 78 33 113 51 NC_002950:PG1942|3end_30S_ribosomal_protein_S12_2027946..2028096_NEGATIVE 303 47 9 44 2 NC_002950:PG1010|3end_ABC_transporter,_ATP-binding_protein_1068509..1068659_NEGATIVE 300 100 57 67 4 NC_002950:PG1133|3end_hypothetical_protein_1214103..1214253_POSITIVE 299 115 71 135 87 NC_002950:PG0718|3end_hypothetical_protein_771136..771286_NEGATIVE 298 118 71 97 43 NC_002950:PG2207|3end_hypothetical_protein_2319142..2319292_NEGATIVE 298 99 51 105 44 NC_002950:PG0320|3end_hypothetical_protein_354577..354727_POSITIVE 295 77 33 151 111 NC_002950:PG1353|3end_orotate_phosphoribosyltransferase_1433201..1433351_NEGATIVE 295 41 1 68 31 NC_002950:PG0022|3end_sulfate_permease_family_protein_25120..25270_NEGATIVE 294 100 51 133 71 NC_002950:PG1174|3end_thioesterase_family_protein_1253210..1253360_NEGATIVE 290 118 73 118 63 NC_002950:PG0767|3end_4-alpha-glucanotransferase_818557..818707_NEGATIVE 290 119 75 60 14 NC_002950:PG0433|3end_tetrapyrrole_methylase_family_protein_470683..470833_NEGATIVE 289 60 12 66 20 NC_002950:PG1449|3end_hypothetical_protein_1533582..1533732_NEGATIVE 289 60 12 83 37 NC_002950:PG1196|3end_hypothetical_protein_1275359..1275509_POSITIVE 289 60 12 66 20 NC_002950:PG0824|3end_hypothetical_protein_881930..882080_POSITIVE