Origin IGS: aaaagtgcacaatttcatggcaaagaaactatctttttggggggcaacattgttattttttagcgtgtctgcactttcacaaaacatattgacggacataagcaaatacgacttgaataaaatagaaaaacggacgattaaaaaaagtcgtaagctcttaaaaacagaaaaagagcgaaagctttttgccttcacgtattttgcaataggattatcggatttttcttctaaaaaaaacggcgatatcgacaattttctcaagaatttcaaattatcaagcaaagggaacagtattgccgatgattttttcgaaaactttaaactctatgattacagatgctatgtatacgacgactcgttgaatataattgcgatggcaatgaaaacaaatgtattcctttttggcagcacaacgccagacagtagatatattgagtatataacaagaataaatcctgaatacacatttgaatacatattttacccgggatcatgctgcatgtatttttgttacaaaaatggtgaaattataattgttcgtttgtctgatgatggtgaaaatattatttcttatcctttatcagattcaaagtaagtagagaaaatgcgtcttgacgaaatctatgacacagcttgcgaaatgaatgagattgaaaaaaataaaagcatcctttcaggggaaaggatatgatttgagaaaaattaaaggatgtggtgatgacacacaatctgtagatattgctcttgagaaacaagaagcacttaggcttcttaaactatattacaacttggggattcctgtattagggggggcgatgtcttttattaaaagatgatggcgaagttgattggacttatgacaattggtatattgatcgattggaaaacgagagcgatattcaattttcaaaaagaatcatagaggaatatcaatctatataagtaattataataatacttatttcaggaatgcatattttttatttgaatttgtatgccaaacgggtacagatcggcaattgctactgcaatagatcgacaaatatggctgcattattaagtttaggtccaacatcagcaggtggttctattcttctcttataagaaacgagattgtgcgcaaataaaaaaacaacaaataagaggtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aacctcttatttgttgtttttttatttgcgcacaatctcgtttcttataagagaagaatagaaccacctgctgatgttggacctaaacttaataatgcagccatatttgtcgatctattgcagtagcaattgccgatctgtacccgtttggcatacaaattcaaataaaaaatatgcattcctgaaataagtattattataattacttatatagattgatattcctctatgattctttttgaaaattgaatatcgctctcgttttccaatcgatcaatataccaattgtcataagtccaatcaacttcgccatcatcttttaataaaagacatcgccccccctaatacaggaatccccaagttgtaatatagtttaagaagcctaagtgcttcttgtttctcaagagcaatatctacagattgtgtgtcatcaccacatcctttaatttttctcaaatcatatcctttcccctgaaaggatgcttttatttttttcaatctcattcatttcgcaagctgtgtcatagatttcgtcaagacgcattttctctacttactttgaatctgataaaggataagaaataatattttcaccatcatcagacaaacgaacaattataatttcaccatttttgtaacaaaaatacatgcagcatgatcccgggtaaaatatgtattcaaatgtgtattcaggatttattcttgttatatactcaatatatctactgtctggcgttgtgctgccaaaaaggaatacatttgttttcattgccatcgcaattatattcaacgagtcgtcgtatacatagcatctgtaatcatagagtttaaagttttcgaaaaaatcatcggcaatactgttccctttgcttgataatttgaaattcttgagaaaattgtcgatatcgccgttttttttagaagaaaaatccgataatcctattgcaaaatacgtgaaggcaaaaagctttcgctctttttctgtttttaagagcttacgactttttttaatcgtccgtttttctattttattcaagtcgtatttgcttatgtccgtcaatatgttttgtgaaagtgcagacacgctaaaaaataacaatgttgccccccaaaaagatagtttctttgccatgaaattgtgcactttt Mask Tandem Repeat Region ================================================ aaaagtgcacaatttcatggcaaagaaactatctttttggggggcaacattgttattttttagcgtgtctgcactttcacaaaacatattgacggacataagcaaatacgacttgaataaaatagaaaaacggacgattaaaaaaagtcgtaagctcttaaaaacagaaaaagagcgaaagctttttgccttcacgtattttgcaataggattatcggatttttcttctaaaaaaaacggcgatatcgacaattttctcaagaatttcaaattatcaagcaaagggaacagtattgccgatgattttttcgaaaactttaaactctatgattacagatgctatgtatacgacgactcgttgaatataattgcgatggcaatgaaaacaaatgtattcctttttggcagcacaacgccagacagtagatatattgagtatataacaagaataaatcctgaatacacatttgaatacatattttacccgggatcatgctgcatgtatttttgttacaaaaatggtgaaattataattgttcgtttgtctgatgatggtgaaaatattatttcttatcctttatcagattcaaagtaagtagagaaaatgcgtcttgacgaaatctatgacacagcttgcgaaatgaatgagattgaaaaaaataaaagcatcctttcaggggaaaggatatgatttgagaaaaattaaaggatgtggtgatgacacacaatctgtagatattgctcttgagaaacaagaagcacttaggcttcttaaactatattacaacttggggattcctgtattagggggggcgatgtcttttattaaaagatgatggcgaagttgattggacttatgacaattggtatattgatcgattggaaaacgagagcgatattcaattttcaaaaagaatcatagaggaatatcaatctatataagtaattataataatacttatttcaggaatgcatattttttatttgaatttgtatgccaaacgggtacagatcggcaattgctactgcaatagatcgacaaatatggctgcattattaagtttaggtccaacatcagcaggtggttctattcttctcttataagaaacgagattgtgcgcaaataaaaaaacaacaaataagaggtt Find is-nt database================================================ Query_seq: PG1892:PG1893|PG1892:PG1893:hypothetical protein:hypothetical protein:->->:1989952..1991099 1148 aaaagtgcacaatttcatggcaaagaaactatctttttggggggcaacattgttattttttagcgtgtctgcactttcacaaaacatattgacggacataagcaaatacgacttgaataaaatagaaaaacggacgattaaaaaaagtcgtaagctcttaaaaacagaaaaagagcgaaagctttttgccttcacgtattttgcaataggattatcggatttttcttctaaaaaaaacggcgatatcgacaattttctcaagaatttcaaattatcaagcaaagggaacagtattgccgatgattttttcgaaaactttaaactctatgattacagatgctatgtatacgacgactcgttgaatataattgcgatggcaatgaaaacaaatgtattcctttttggcagcacaacgccagacagtagatatattgagtatataacaagaataaatcctgaatacacatttgaatacatattttacccgggatcatgctgcatgtatttttgttacaaaaatggtgaaattataattgttcgtttgtctgatgatggtgaaaatattatttcttatcctttatcagattcaaagtaagtagagaaaatgcgtcttgacgaaatctatgacacagcttgcgaaatgaatgagattgaaaaaaataaaagcatcctttcaggggaaaggatatgatttgagaaaaattaaaggatgtggtgatgacacacaatctgtagatattgctcttgagaaacaagaagcacttaggcttcttaaactatattacaacttggggattcctgtattagggggggcgatgtcttttattaaaagatgatggcgaagttgattggacttatgacaattggtatattgatcgattggaaaacgagagcgatattcaattttcaaaaagaatcatagaggaatatcaatctatataagtaattataataatacttatttcaggaatgcatattttttatttgaatttgtatgccaaacgggtacagatcggcaattgctactgcaatagatcgacaaatatggctgcattattaagtttaggtccaacatcagcaggtggttctattcttctcttataagaaacgagattgtgcgcaaataaaaaaacaacaaataagaggtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1892:PG1893|PG1892:PG1893:hypothetical protein:hypothetical protein:->->:1989952..1991099 1148 aaaagtgcacaatttcatggcaaagaaactatctttttggggggcaacattgttattttttagcgtgtctgcactttcacaaaacatattgacggacataagcaaatacgacttgaataaaatagaaaaacggacgattaaaaaaagtcgtaagctcttaaaaacagaaaaagagcgaaagctttttgccttcacgtattttgcaataggattatcggatttttcttctaaaaaaaacggcgatatcgacaattttctcaagaatttcaaattatcaagcaaagggaacagtattgccgatgattttttcgaaaactttaaactctatgattacagatgctatgtatacgacgactcgttgaatataattgcgatggcaatgaaaacaaatgtattcctttttggcagcacaacgccagacagtagatatattgagtatataacaagaataaatcctgaatacacatttgaatacatattttacccgggatcatgctgcatgtatttttgttacaaaaatggtgaaattataattgttcgtttgtctgatgatggtgaaaatattatttcttatcctttatcagattcaaagtaagtagagaaaatgcgtcttgacgaaatctatgacacagcttgcgaaatgaatgagattgaaaaaaataaaagcatcctttcaggggaaaggatatgatttgagaaaaattaaaggatgtggtgatgacacacaatctgtagatattgctcttgagaaacaagaagcacttaggcttcttaaactatattacaacttggggattcctgtattagggggggcgatgtcttttattaaaagatgatggcgaagttgattggacttatgacaattggtatattgatcgattggaaaacgagagcgatattcaattttcaaaaagaatcatagaggaatatcaatctatataagtaattataataatacttatttcaggaatgcatattttttatttgaatttgtatgccaaacgggtacagatcggcaattgctactgcaatagatcgacaaatatggctgcattattaagtttaggtccaacatcagcaggtggttctattcttctcttataagaaacgagattgtgcgcaaataaaaaaacaacaaataagaggtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1892:PG1893|PG1892:PG1893:hypothetical protein:hypothetical protein:->->:1989952..1991099 1148 aaaagtgcacaatttcatggcaaagaaactatctttttggggggcaacattgttattttttagcgtgtctgcactttcacaaaacatattgacggacataagcaaatacgacttgaataaaatagaaaaacggacgattaaaaaaagtcgtaagctcttaaaaacagaaaaagagcgaaagctttttgccttcacgtattttgcaataggattatcggatttttcttctaaaaaaaacggcgatatcgacaattttctcaagaatttcaaattatcaagcaaagggaacagtattgccgatgattttttcgaaaactttaaactctatgattacagatgctatgtatacgacgactcgttgaatataattgcgatggcaatgaaaacaaatgtattcctttttggcagcacaacgccagacagtagatatattgagtatataacaagaataaatcctgaatacacatttgaatacatattttacccgggatcatgctgcatgtatttttgttacaaaaatggtgaaattataattgttcgtttgtctgatgatggtgaaaatattatttcttatcctttatcagattcaaagtaagtagagaaaatgcgtcttgacgaaatctatgacacagcttgcgaaatgaatgagattgaaaaaaataaaagcatcctttcaggggaaaggatatgatttgagaaaaattaaaggatgtggtgatgacacacaatctgtagatattgctcttgagaaacaagaagcacttaggcttcttaaactatattacaacttggggattcctgtattagggggggcgatgtcttttattaaaagatgatggcgaagttgattggacttatgacaattggtatattgatcgattggaaaacgagagcgatattcaattttcaaaaagaatcatagaggaatatcaatctatataagtaattataataatacttatttcaggaatgcatattttttatttgaatttgtatgccaaacgggtacagatcggcaattgctactgcaatagatcgacaaatatggctgcattattaagtttaggtccaacatcagcaggtggttctattcttctcttataagaaacgagattgtgcgcaaataaaaaaacaacaaataagaggtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaagtgcacaatttcatggcaaagaaactatctttttggggggcaacattgttattttttagcgtgtctgcactttcacaaaacatattgacggacataagcaaatacgacttgaataaaatagaaaaacggacgattaaaaaaagtcgtaagctcttaaaaacagaaaaagagcgaaagctttttgccttcacgtattttgcaataggattatcggatttttcttctaaaaaaaacggcgatatcgacaattttctcaagaatttcaaattatcaagcaaagggaacagtattgccgatgattttttcgaaaactttaaactctatgattacagatgctatgtatacgacgactcgttgaatataattgcgatggcaatgaaaacaaatgtattcctttttggcagcacaacgccagacagtagatatattgagtatataacaagaataaatcctgaatacacatttgaatacatattttacccgggatcatgctgcatgtatttttgttacaaaaatggtgaaattataattgttcgtttgtctgatgatggtgaaaatattatttcttatcctttatcagattcaaagtaagtagagaaaatgcgtcttgacgaaatctatgacacagcttgcgaaatgaatgagattgaaaaaaataaaagcatcctttcaggggaaaggatatgatttgagaaaaattaaaggatgtggtgatgacacacaatctgtagatattgctcttgagaaacaagaagcacttaggcttcttaaactatattacaacttggggattcctgtattagggggggcgatgtcttttattaaaagatgatggcgaagttgattggacttatgacaattggtatattgatcgattggaaaacgagagcgatattcaattttcaaaaagaatcatagaggaatatcaatctatataagtaattataataatacttatttcaggaatgcatattttttatttgaatttgtatgccaaacgggtacagatcggcaattgctactgcaatagatcgacaaatatggctgcattattaagtttaggtccaacatcagcaggtggttctattcttctcttataagaaacgagattgtgcgcaaataaaaaaacaacaaataagaggtt Predict ORF larger than 30AA ================================================ Protein_Len: 47 Strand: + Start: 219 End: 359 .......................................................................................................................................................................................................................... M F L L K K T A I S T I F S R I S N Y Q A K G T V L P M I F S K T L N S M I T D A M Y T T T R ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 5 End: 592 .... M H N F M A K K L S F W G A T L L F F S V S A L S Q N I L T D I S K Y D L N K I E K R T I K K S R K L L K T E K E R K L ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 645 End: 833 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M R L K K I K A S F Q G K G Y D L R K I K G C G D D T Q S V D I A L E K Q E A L R L L K L Y Y N L G I P V L G G A M S F ........................................................................................................................................................................................................................................................................................................................... Protein_Len: 51 Strand: + Start: 879 End: 1031 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M G K R E R Y S I F K K N H R G I S I Y I S N Y N N T Y F R N A Y F L F E F V C Q T G T D R Q L L L Q ..................................................................................................................... Protein_Len: 52 Strand: + Start: 946 End: 1101 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M I I I L I S G M H I F Y L N L Y A K R V Q I G N C Y C N R S T N M A A L L S L G P T S A G G S I L L L ............................................... Protein_Len: 30 Strand: + Start: 1035 End: 1124 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M D K Y G C I I K F R S N I S R W F Y S S L I R N E I V R K ........................ Protein_Len: 40 Strand: - Start: 962 End: 1081 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. K L F A Y K K N S N T H W V P V S R C N S S C Y I S L Y P Q M I L N L D L M L M ................................................................... Protein_Len: 42 Strand: - Start: 785 End: 910 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ L K P I G T N P P A I D K I L L H H R L Q N S K H C N T Y Q D I P F R S R Y E I K M .............................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 567 End: 755 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... K I L N L T L L S F A D Q R F R H C L K R F S H S Q F F Y F C G K L P F S I I Q S F N F S T T I V C L R Y I N S K L F M ......................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 45 Strand: - Start: 343 End: 477 ...................................................................................................................................................................................................................................................................................................................................................... T Y S S E N F I I A I A I F V F T N R K P L V V G S L L Y I S Y I V L I F G S Y V N S Y M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 58 Strand: - Start: 321 End: 494 ................................................................................................................................................................................................................................................................................................................................ V R H N C I S H I R R S T S Y L Q S P L S F L H I G K Q C C L A L C Y I Y Q T Y L L F L D Q I C M Q I C I K G P I M .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 59 Strand: - Start: 278 End: 454 ..................................................................................................................................................................................................................................................................................... A F P V T N G I I K E F V K F E I I V S A I Y V V V R Q I Y N R H C H F C I Y E K K A A C R W V T S I N L I Y C S Y M ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 1 End: 294 F L I S F R V I L F L R L S K F V S F S R F S K A K V Y K A I P N D S K E E L F F P S I S L K R L F K L N D L L P F L M ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... aaaagtgcacaatttcatggcaaagaaactatctttttggggggcaacattgttattttttagcgtgtctgcactttcacaaaacatattgacggacataagcaaatacgacttgaataaaatagaaaaacggacgattaaaaaaagtcgtaagctcttaaaaacagaaaaagagcgaaagctttttgccttcacgtattttgcaataggattatcggatttttcttctaaaaaaaacggcgatatcgacaattttctcaagaatttcaaattatcaagcaaagggaacagtattgccgatgattttttcgaaaactttaaactctatgattacagatgctatgtatacgacgactcgttgaatataattgcgatggcaatgaaaacaaatgtattcctttttggcagcacaacgccagacagtagatatattgagtatataacaagaataaatcctgaatacacatttgaatacatattttacccgggatcatgctgcatgtatttttgttacaaaaatggtgaaattataattgttcgtttgtctgatgatggtgaaaatattatttcttatcctttatcagattcaaagtaagtagagaaaatgcgtcttgacgaaatctatgacacagcttgcgaaatgaatgagattgaaaaaaataaaagcatcctttcaggggaaaggatatgatttgagaaaaattaaaggatgtggtgatgacacacaatctgtagatattgctcttgagaaacaagaagcacttaggcttcttaaactatattacaacttggggattcctgtattagggggggcgatgtcttttattaaaagatgatggcgaagttgattggacttatgacaattggtatattgatcgattggaaaacgagagcgatattcaattttcaaaaagaatcatagaggaatatcaatctatataagtaattataataatacttatttcaggaatgcatattttttatttgaatttgtatgccaaacgggtacagatcggcaattgctactgcaatagatcgacaaatatggctgcattattaagtttaggtccaacatcagcaggtggttctattcttctcttataagaaacgagattgtgcgcaaataaaaaaacaacaaataagaggtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaagtgcacaatttcatggcaaagaaactatctttttggggggcaacattgttattttttagcgtgtctgcactttcacaaaacatattgacggacataagcaaatacgacttgaataaaatagaaaaacggacgattaaaaaaagtcgtaagctcttaaaaacagaaaaagagcgaaagctttttgccttcacgtattttgcaataggattatcggatttttcttctaaaaaaaacggcgatatcgacaattttctcaagaatttcaaattatcaagcaaagggaacagtattgccgatgattttttcgaaaactttaaactctatgattacagatgctatgtatacgacgactcgttgaatataattgcgatggcaatgaaaacaaatgtattcctttttggcagcacaacgccagacagtagatatattgagtatataacaagaataaatcctgaatacacatttgaatacatattttacccgggatcatgctgcatgtatttttgttacaaaaatggtgaaattataattgttcgtttgtctgatgatggtgaaaatattatttcttatcctttatcagattcaaagtaagtagagaaaatgcgtcttgacgaaatctatgacacagcttgcgaaatgaatgagattgaaaaaaataaaagcatcctttcaggggaaaggatatgatttgagaaaaattaaaggatgtggtgatgacacacaatctgtagatattgctcttgagaaacaagaagcacttaggcttcttaaactatattacaacttggggattcctgtattagggggggcgatgtcttttattaaaagatgatggcgaagttgattggacttatgacaattggtatattgatcgattggaaaacgagagcgatattcaattttcaaaaagaatcatagaggaatatcaatctatataagtaattataataatacttatttcaggaatgcatattttttatttgaatttgtatgccaaacgggtacagatcggcaattgctactgcaatagatcgacaaatatggctgcattattaagtttaggtccaacatcagcaggtggttctattcttctcttataagaaacgagattgtgcgcaaataaaaaaacaacaaataagaggtt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 69 HP_score: -5.4 Tail_Score: -4.2778 Start: 175 End: 195 Full_Region: cctattgcaaaatac gtgaaggc aaaaa gctttcgc tctttttctgttttt ..............................................................................................................................................................................gtgaaggcaaaaagctttcgc......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 47 HP_score: -2.1 Tail_Score: -3.55714 Start: 444 End: 458 Full_Region: attcaaatgtgtatt cagga tttat tcttg ttatatactcaatat ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................caggatttattcttg.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 76 HP_score: -2.6 Tail_Score: -5.50499 Start: 661 End: 676 Full_Region: tcatatcctttcccc tgaaag gatg ctttta tttttttcaatctca ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgaaaggatgctttta........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 82 HP_score: -4.6 Tail_Score: -5.55855 Start: 666 End: 689 Full_Region: aatttttctcaaatc atatcctttc ccct gaaaggatgc ttttatttttttcaa .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................atatcctttcccctgaaaggatgc........................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 79 HP_score: -8.1 Tail_Score: -3.91073 Start: 667 End: 688 Full_Region: atttttctcaaatca tatcctttc ccct gaaaggatg cttttatttttttca ..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tatcctttcccctgaaaggatg............................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 71 HP_score: -8.5 Tail_Score: -3.00525 Start: 669 End: 686 Full_Region: AAAAAAATAAAAGCA TCCTTTC AGGG GAAAGGA TATGATTTGAGAAAA ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TCCTTTCAGGGGAAAGGA.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 75 HP_score: -8.5 Tail_Score: -3.49323 Start: 669 End: 686 Full_Region: ttttctcaaatcata tcctttc ccct gaaagga tgcttttattttttt ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tcctttcccctgaaagga.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 61 HP_score: -5.2 Tail_Score: -3.85817 Start: 753 End: 774 Full_Region: agttgtaatatagtt taagaagc ctaagt gcttcttg tttctcaagagcaat ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................taagaagcctaagtgcttcttg...................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 62 HP_score: -4.7 Tail_Score: -4.06419 Start: 756 End: 771 Full_Region: tgtaatatagtttaa gaagc ctaagt gcttc ttgtttctcaagagc ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gaagcctaagtgcttc......................................................................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: PG1892:PG1893|PG1892:PG1893:hypothetical protein:hypothetical protein:->->:1989952..1991099 1148 aaaagtgcacaatttcatggcaaagaaactatctttttggggggcaacattgttattttttagcgtgtctgcactttcacaaaacatattgacggacataagcaaatacgacttgaataaaatagaaaaacggacgattaaaaaaagtcgtaagctcttaaaaacagaaaaagagcgaaagctttttgccttcacgtattttgcaataggattatcggatttttcttctaaaaaaaacggcgatatcgacaattttctcaagaatttcaaattatcaagcaaagggaacagtattgccgatgattttttcgaaaactttaaactctatgattacagatgctatgtatacgacgactcgttgaatataattgcgatggcaatgaaaacaaatgtattcctttttggcagcacaacgccagacagtagatatattgagtatataacaagaataaatcctgaatacacatttgaatacatattttacccgggatcatgctgcatgtatttttgttacaaaaatggtgaaattataattgttcgtttgtctgatgatggtgaaaatattatttcttatcctttatcagattcaaagtaagtagagaaaatgcgtcttgacgaaatctatgacacagcttgcgaaatgaatgagattgaaaaaaataaaagcatcctttcaggggaaaggatatgatttgagaaaaattaaaggatgtggtgatgacacacaatctgtagatattgctcttgagaaacaagaagcacttaggcttcttaaactatattacaacttggggattcctgtattagggggggcgatgtcttttattaaaagatgatggcgaagttgattggacttatgacaattggtatattgatcgattggaaaacgagagcgatattcaattttcaaaaagaatcatagaggaatatcaatctatataagtaattataataatacttatttcaggaatgcatattttttatttgaatttgtatgccaaacgggtacagatcggcaattgctactgcaatagatcgacaaatatggctgcattattaagtttaggtccaacatcagcaggtggttctattcttctcttataagaaacgagattgtgcgcaaataaaaaaacaacaaataagaggtt Intra-Species Hit: Count: 1 Min: 1 Max: 1148 Len: 1148 Subject: NC_002950_PG1892_PG1893|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1989952,1991099]|1148 HSP 1 e-value: 0.0 bit: 1744.0 Len: 967 Query Start:182 Query End:1148 Subject Strand: POSITIVE Subject Start: 182 Subject End: 1148 .....................................................................................................................................................................................ctttttgccttcacgtattttgcaataggattatcggatttttcttctnnnnnnnncggcgatatcgacaattttctcaagaatttcaaattatcaagcaaagggaacagtattgccgatgattttttcgaaaactttaaactctatgattacagatgctatgtatacgacgactcgttgaatataattgcgatggcaatgaaaacaaatgtattcctttttggcagcacaacgccagacagtagatatattgagtatataacaagaataaatcctgaatacacatttgaatacatattttacccgggatcatgctgcatgtatttttgttacaaaaatggtgaaattataattgttcgtttgtctgatgatggtgaaaatattatttcttatcctttatcagattcaaagtaagtagagaaaatgcgtcttgacgaaatctatgacacagcttgcgaaatgaatgagattgnnnnnnntaaaagcatcctttcaggggaaaggatatgatttgagaaaaattaaaggatgtggtgatgacacacaatctgtagatattgctcttgagaaacaagaagcacttaggcttcttaaactatattacaacttggggattcctgtattannnnnnncgatgtcttttattaaaagatgatggcgaagttgattggacttatgacaattggtatattgatcgattggaaaacgagagcgatattcaattttcaaaaagaatcatagaggaatatcaatctatataagtaattataataatacttatttcaggaatgcatattttttatttgaatttgtatgccaaacgggtacagatcggcaattgctactgcaatagatcgacaaatatggctgcattattaagtttaggtccaacatcagcaggtggttctattcttctcttataagaaacgagattgtgcgcaaatnnnnnnncaacaaataagaggtt .....................................................................................................................................................................................ctttttgccttcacgtattttgcaataggattatcggatttttcttctaaaaaaaacggcgatatcgacaattttctcaagaatttcaaattatcaagcaaagggaacagtattgccgatgattttttcgaaaactttaaactctatgattacagatgctatgtatacgacgactcgttgaatataattgcgatggcaatgaaaacaaatgtattcctttttggcagcacaacgccagacagtagatatattgagtatataacaagaataaatcctgaatacacatttgaatacatattttacccgggatcatgctgcatgtatttttgttacaaaaatggtgaaattataattgttcgtttgtctgatgatggtgaaaatattatttcttatcctttatcagattcaaagtaagtagagaaaatgcgtcttgacgaaatctatgacacagcttgcgaaatgaatgagattgaaaaaaataaaagcatcctttcaggggaaaggatatgatttgagaaaaattaaaggatgtggtgatgacacacaatctgtagatattgctcttgagaaacaagaagcacttaggcttcttaaactatattacaacttggggattcctgtattagggggggcgatgtcttttattaaaagatgatggcgaagttgattggacttatgacaattggtatattgatcgattggaaaacgagagcgatattcaattttcaaaaagaatcatagaggaatatcaatctatataagtaattataataatacttatttcaggaatgcatattttttatttgaatttgtatgccaaacgggtacagatcggcaattgctactgcaatagatcgacaaatatggctgcattattaagtttaggtccaacatcagcaggtggttctattcttctcttataagaaacgagattgtgcgcaaataaaaaaacaacaaataagaggtt HSP 2 e-value: 2.0E-59 bit: 232.0 Len: 117 Query Start:1 Query End:117 Subject Strand: POSITIVE Subject Start: 1 Subject End: 117 aaaagtgcacaatttcatggcaaagaaactatctttttggggggcaacattgttattttttagcgtgtctgcactttcacaaaacatattgacggacataagcaaatacgacttgaa....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... aaaagtgcacaatttcatggcaaagaaactatctttttggggggcaacattgttattttttagcgtgtctgcactttcacaaaacatattgacggacataagcaaatacgacttgaa....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAGUGCACAAUUUCAUGGCAAAGAAACUAUCUUUUUGGGGGGCAACAUUGUUAUUUUUUAGCGUGUCUGCACUUUCACAAAACAUAUUGACGGACAUAAGCAAAUACGACUUGAAUAAAAUAGAAAAACGGACGAUUAAAAAAAGUCGUAAGCUCUUAAAAACAGAAAAAGAGCGAAAGCUUUUUGCCUUCACGUAUUUUGCAAUAGGAUUAUCGGAUUUUUCUUCUAAAAAAAACGGCGAUAUCGACAAUUUUCUCAAGAAUUUCAAAUUAUCAAGCAAAGGGAACAGUAUUGCCGAUGAUUUUUUCGAAAACUUUAAACUCUAUGAUUACAGAUGCUAUGUAUACGACGACUCGUUGAAUAUAAUUGCGAUGGCAAUGAAAACAAAUGUAUUCCUUUUUGGCAGCACAACGCCAGACAGUAGAUAUAUUGAGUAUAUAACAAGAAUAAAUCCUGAAUACACAUUUGAAUACAUAUUUUACCCGGGAUCAUGCUGCAUGUAUUUUUGUUACAAAAAUGGUGAAAUUAUAAUUGUUCGUUUGUCUGAUGAUGGUGAAAAUAUUAUUUCUUAUCCUUUAUCAGAUUCAAAGUAAGUAGAGAAAAUGCGUCUUGACGAAAUCUAUGACACAGCUUGCGAAAUGAAUGAGAUUGAAAAAAAUAAAAGCAUCCUUUCAGGGGAAAGGAUAUGAUUUGAGAAAAAUUAAAGGAUGUGGUGAUGACACACAAUCUGUAGAUAUUGCUCUUGAGAAACAAGAAGCACUUAGGCUUCUUAAACUAUAUUACAACUUGGGGAUUCCUGUAUUAGGGGGGGCGAUGUCUUUUAUUAAAAGAUGAUGGCGAAGUUGAUUGGACUUAUGACAAUUGGUAUAUUGAUCGAUUGGAAAACGAGAGCGAUAUUCAAUUUUCAAAAAGAAUCAUAGAGGAAUAUCAAUCUAUAUAAGUAAUUAUAAUAAUACUUAUUUCAGGAAUGCAUAUUUUUUAUUUGAAUUUGUAUGCCAAACGGGUACAGAUCGGCAAUUGCUACUGCAAUAGAUCGACAAAUAUGGCUGCAUUAUUAAGUUUAGGUCCAACAUCAGCAGGUGGUUCUAUUCUUCUCUUAUAAGAAACGAGAUUGUGCGCAAAUAAAAAAACAACAAAUAAGAGGUU ....((((((((((((.......((((.((((((...((..(((((((((.((((.....)))))))).))).))..))..............((((.(((((.........(..(((((((..(((...(..((((((......))))))..)...............(((((((....)))))))...)))..(((((((..((((((((((..(((((.((((((((.......(((((((((.((.(((((......)))))))...............(....).)))))))))..((((((((.(((((................(((((((....(((.(((.((..((.((((.((((.(((((....))))).......((((((((.((.((((((........)))))).))..))))))))..)))).)))).)).....(((((.........((..(((.((((((((.((((..(..(((((.(((.((((((((...)))))))))))...(((...(..((((((((((((((((.((.((((......))))....)).))))))))).....((((((........((.(((..((.....))...))).)))))))).)))))))..)...)))..........)))))..)..).)))...)))))))))))..)).........))))))).)))...)))....)))))))(((((((((((((......(((((((......)))))))...........((((((.....(((((......))))).((.((((((((.....)))))).)).)).)))))).............((((((((......)))))))).....)))))))))))))...)))))..)))))))).))))))))....))))).(((((....))))).)))).))))))...))))))).....)))))))..)...((((.((((.....((...((((....(((((....))))).)))).)).....)))))))).......))))).)))).........)))))))))).(((((.......)))))..))))))))))))........................... (-272.37) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACCUCUUAUUUGUUGUUUUUUUAUUUGCGCACAAUCUCGUUUCUUAUAAGAGAAGAAUAGAACCACCUGCUGAUGUUGGACCUAAACUUAAUAAUGCAGCCAUAUUUGUCGAUCUAUUGCAGUAGCAAUUGCCGAUCUGUACCCGUUUGGCAUACAAAUUCAAAUAAAAAAUAUGCAUUCCUGAAAUAAGUAUUAUUAUAAUUACUUAUAUAGAUUGAUAUUCCUCUAUGAUUCUUUUUGAAAAUUGAAUAUCGCUCUCGUUUUCCAAUCGAUCAAUAUACCAAUUGUCAUAAGUCCAAUCAACUUCGCCAUCAUCUUUUAAUAAAAGACAUCGCCCCCCCUAAUACAGGAAUCCCCAAGUUGUAAUAUAGUUUAAGAAGCCUAAGUGCUUCUUGUUUCUCAAGAGCAAUAUCUACAGAUUGUGUGUCAUCACCACAUCCUUUAAUUUUUCUCAAAUCAUAUCCUUUCCCCUGAAAGGAUGCUUUUAUUUUUUUCAAUCUCAUUCAUUUCGCAAGCUGUGUCAUAGAUUUCGUCAAGACGCAUUUUCUCUACUUACUUUGAAUCUGAUAAAGGAUAAGAAAUAAUAUUUUCACCAUCAUCAGACAAACGAACAAUUAUAAUUUCACCAUUUUUGUAACAAAAAUACAUGCAGCAUGAUCCCGGGUAAAAUAUGUAUUCAAAUGUGUAUUCAGGAUUUAUUCUUGUUAUAUACUCAAUAUAUCUACUGUCUGGCGUUGUGCUGCCAAAAAGGAAUACAUUUGUUUUCAUUGCCAUCGCAAUUAUAUUCAACGAGUCGUCGUAUACAUAGCAUCUGUAAUCAUAGAGUUUAAAGUUUUCGAAAAAAUCAUCGGCAAUACUGUUCCCUUUGCUUGAUAAUUUGAAAUUCUUGAGAAAAUUGUCGAUAUCGCCGUUUUUUUUAGAAGAAAAAUCCGAUAAUCCUAUUGCAAAAUACGUGAAGGCAAAAAGCUUUCGCUCUUUUUCUGUUUUUAAGAGCUUACGACUUUUUUUAAUCGUCCGUUUUUCUAUUUUAUUCAAGUCGUAUUUGCUUAUGUCCGUCAAUAUGUUUUGUGAAAGUGCAGACACGCUAAAAAAUAACAAUGUUGCCCCCCAAAAAGAUAGUUUCUUUGCCAUGAAAUUGUGCACUUUU ..........((((((((((((.....(((.....(((..((((((....))))))...))).....((((.((((((((((..((((.(((((((((.((((.((..(((((((.(((((....)))))....)))).).))..)).)))).......((((...................))))....))))))))).............(((.((((((((....((..(((.....))).)).)))))))).))).)))).......((.((((......))))))....))))))..........))))..((((((....(((((((.............(((((((........((((((((.....((((((((......))))))))((((((((((........(((((((((((((((........((((((((...............(((((((((....)))))))))................((..(((((...(....).((((((...(((...)))..))))))................)))))..))))))))))..((((......))))..........(((....((((((.....(((((...(((((((...)))))))....((((((..((.((((..(....(((((((.....((((((.(((((.....)))))..))))))..)))))))....)..)))).))..))))))......)))))....))))))..(((((....)))))............)))...).)))))))..))))))).................(((((((...((((..(((((...........)))))))))..))))))).))))))))))......(((((((..((((((((....)))))))).)))).))).)))))))).....((((((((.....))))))))....)))))))......((((.((((((((....(((.................)))...))))))))...))))............))))))).)))))).))))...))).))))))))))))............(((((((((((((........))))))))...))))) (-233.64) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 360 220 173 197 145 NC_002950:PG1733|5end_hypothetical_protein_1821623..1821853_NEGATIVE 347 846 803 176 124 NC_002950:PG2104|5end_hypothetical_protein_2213619..2213849_NEGATIVE 344 421 391 176 146 NC_002950:PG1074|5end_D-lysine_5,6-aminomutase,_beta_subunit_1141963..1142193_POSITIVE 337 847 803 152 106 NC_002950:PG2036|5end_ion_transporter_2136561..2136791_POSITIVE 326 825 788 179 144 NC_002950:PG0938|5end_calcium-transporting_ATPase_1000385..1000615_NEGATIVE 317 824 791 85 53 NC_002950:PG1283|5end_hypothetical_protein_1360553..1360783_POSITIVE 316 1119 1081 133 97 NC_002950:PG0082|5end_hypothetical_protein_96044..96274_POSITIVE 315 1049 1003 118 70 NC_002950:PG0447|5end_hypothetical_protein_487278..487508_POSITIVE 311 1039 991 60 18 NC_002950:PG0480|5end_precorrin-2_C20-methyltransferase,_putative_521319..521549_NEGATIVE 307 1037 1003 107 72 NC_002950:PG1874|5end_hypothetical_protein_1969660..1969890_NEGATIVE 305 1027 985 170 127 NC_002950:PG1898|5end_transporter,_putative_1997335..1997565_NEGATIVE 304 1050 1003 131 77 NC_002950:PG1594|5end_ComEC/Rec2-related_protein_1673587..1673817_NEGATIVE 302 1051 1012 71 32 NC_002950:PG1005|5end_lipoprotein,_putative_1063763..1063993_NEGATIVE 302 340 296 63 7 NC_002950:PG0296|5end_phosphoribosylformylglycinamidine_synthase_334997..335227_NEGATIVE 301 71 38 203 164 NC_002950:PG2209|5end_hypothetical_protein_2320928..2321158_NEGATIVE 301 842 801 74 26 NC_002950:PG0799|5end_hypothetical_protein_854334..854564_POSITIVE 300 1038 1004 44 10 NC_002950:PG0135|5end_dimethyladenosine_transferase_157943..158173_NEGATIVE 297 1027 996 128 103 NC_002950:PG1735|5end_MutT/nudix_family_protein_1823082..1823312_NEGATIVE 294 1040 993 205 159 NC_002950:PG1125|5end_hypothetical_protein_1201727..1201957_NEGATIVE 293 838 791 164 118 NC_002950:PG1588|5end_hypothetical_protein_1666532..1666762_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 337 847 803 120 74 NC_002950:PG2035|3end_tRNA_(guanine-N(1)-)-methyltransferase_2136609..2136759_POSITIVE 329 1049 1001 104 59 NC_002950:PG0003|3end_hypothetical_protein_1919..2069_NEGATIVE 316 1119 1081 71 35 NC_002950:PG0081|3end_hypothetical_protein_96062..96212_POSITIVE 315 1049 1003 146 98 NC_002950:PG0446|3end_thiF_protein_487386..487536_POSITIVE 314 823 785 62 27 NC_002950:PG1290|3end_branched-chain_amino_acid_aminotransferase_1369352..1369502_POSITIVE 312 1038 995 52 8 NC_002950:PG1742|3end_hypothetical_protein_1827506..1827656_POSITIVE 308 840 795 126 80 NC_002950:PG1814|3end_DNA_primase_1908606..1908756_NEGATIVE 307 1037 1003 120 85 NC_002950:PG1875|3end_hemolysin_1969647..1969797_NEGATIVE 304 1050 1003 124 70 NC_002950:PG1595|3end_ribulose-phosphate_3-epimerase_1673594..1673744_NEGATIVE 303 1027 992 38 3 NC_002950:PG0956|3end_peptidase,_M23/M37_family,_putative_1017172..1017322_POSITIVE 302 1051 1012 40 1 NC_002950:PG1006|3end_hypothetical_protein_1063794..1063944_NEGATIVE 302 855 813 59 7 NC_002950:PG0165|3end_heat_shock_protein_15_187370..187520_NEGATIVE 294 820 790 132 104 NC_002950:PG1711|3end_alpha-1,2-mannosidase_family_protein_1796652..1796802_NEGATIVE 293 838 791 103 57 NC_002950:PG1589|3end_dihydropteroate_synthase_1666593..1666743_NEGATIVE 293 377 340 63 32 NC_002950:PG0588|3end_3-methyl-2-oxobutanoate_hydroxymethyltransferase_645296..645446_NEGATIVE 292 1050 1002 65 25 NC_002950:PG0290|3end_hypothetical_protein_325534..325684_POSITIVE 291 437 404 44 3 NC_002950:PG1393|3end_penicillin-binding_protein_2,_putative_1474406..1474556_NEGATIVE 289 820 788 136 101 NC_002950:PG0033|3end_RmuC_domain_protein_40242..40392_NEGATIVE 288 1049 1006 52 1 NC_002950:PG1653|3end_hypothetical_protein_1736458..1736608_POSITIVE 286 842 807 71 42 NC_002950:PG0926|3end_hypothetical_protein_984407..984557_NEGATIVE