Origin IGS: atccgcacattttactcagtagagtgagtatttttactcagtgcaaatataataaagacatttgattcataatcaatctcttacaagtcaataaaggcaatgtgattatcccataaagggtgagatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatctcaccctttatgggataatcacattgcctttattgacttgtaagagattgattatgaatcaaatgtctttattatatttgcactgagtaaaaatactcactctactgagtaaaatgtgcggat Mask Tandem Repeat Region ================================================ atccgcacattttactcagtagagtgagtatttttactcagtgcaaatataataaagacatttgattcataatcaatctcttacaagtcaataaaggcaatgtgattatcccataaagggtgagatta Find is-nt database================================================ Query_seq: PG1860:PG1861|PG1860:PG1861:hypothetical protein:hypothetical protein:<-<-:1959849..1959976 128 atccgcacattttactcagtagagtgagtatttttactcagtgcaaatataataaagacatttgattcataatcaatctcttacaagtcaataaaggcaatgtgattatcccataaagggtgagatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1860:PG1861|PG1860:PG1861:hypothetical protein:hypothetical protein:<-<-:1959849..1959976 128 atccgcacattttactcagtagagtgagtatttttactcagtgcaaatataataaagacatttgattcataatcaatctcttacaagtcaataaaggcaatgtgattatcccataaagggtgagatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1860:PG1861|PG1860:PG1861:hypothetical protein:hypothetical protein:<-<-:1959849..1959976 128 atccgcacattttactcagtagagtgagtatttttactcagtgcaaatataataaagacatttgattcataatcaatctcttacaagtcaataaaggcaatgtgattatcccataaagggtgagatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atccgcacattttactcagtagagtgagtatttttactcagtgcaaatataataaagacatttgattcataatcaatctcttacaagtcaataaaggcaatgtgattatcccataaagggtgagatta Predict ORF larger than 30AA ================================================ atccgcacattttactcagtagagtgagtatttttactcagtgcaaatataataaagacatttgattcataatcaatctcttacaagtcaataaaggcaatgtgattatcccataaagggtgagatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atccgcacattttactcagtagagtgagtatttttactcagtgcaaatataataaagacatttgattcataatcaatctcttacaagtcaataaaggcaatgtgattatcccataaagggtgagatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG1860:PG1861|PG1860:PG1861:hypothetical protein:hypothetical protein:<-<-:1959849..1959976 128 atccgcacattttactcagtagagtgagtatttttactcagtgcaaatataataaagacatttgattcataatcaatctcttacaagtcaataaaggcaatgtgattatcccataaagggtgagatta Intra-Species Hit: Count: 1 Min: 1 Max: 128 Len: 128 Subject: NC_002950_PG1860_PG1861|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1959849,1959976]|128 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:1 Query End:128 Subject Strand: POSITIVE Subject Start: 1 Subject End: 128 atccgcacattttactcagtagagtgagtatttttactcagtgcaaatataataaagacatttgattcataatcaatctcttacaagtcaataaaggcaatgtgattatcccataaagggtgagatta atccgcacattttactcagtagagtgagtatttttactcagtgcaaatataataaagacatttgattcataatcaatctcttacaagtcaataaaggcaatgtgattatcccataaagggtgagatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCCGCACAUUUUACUCAGUAGAGUGAGUAUUUUUACUCAGUGCAAAUAUAAUAAAGACAUUUGAUUCAUAAUCAAUCUCUUACAAGUCAAUAAAGGCAAUGUGAUUAUCCCAUAAAGGGUGAGAUUA ....((((.....((((....))))(((((....))))).))))..............................((((((.((((.(((......)))..)))).....(((.....))).)))))). (-26.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCUCACCCUUUAUGGGAUAAUCACAUUGCCUUUAUUGACUUGUAAGAGAUUGAUUAUGAAUCAAAUGUCUUUAUUAUAUUUGCACUGAGUAAAAAUACUCACUCUACUGAGUAAAAUGUGCGGAU (((((((.(((.....)))......(((..(.(......).).)))..))))))).........................((((((((.(((((....)))))((((....)))).....)))))))) (-24.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 818 50 1 141 92 NC_002950:PG1860|5end_hypothetical_protein_1959758..1959988_NEGATIVE 546 60 11 145 96 NC_002950:PG1864|5end_leucine-rich_protein_1964309..1964539_NEGATIVE 229 45 2 51 12 NC_002950:PG1985|5end_CRISPR-associated_TM1792_family_protein_2074580..2074810_NEGATIVE 222 47 2 209 168 NC_002950:PG0615|5end_GTP-binding_protein_TypA_667033..667263_POSITIVE 216 30 1 51 23 NC_002950:PG0021|5end_TIM-barrel_protein,_putative,_NifR3_family_25123..25353_NEGATIVE 215 70 23 91 44 NC_002950:PG1788|5end_cysteine_peptidase,_putative_1875976..1876206_POSITIVE 213 120 97 151 126 NC_002950:PG0160|5end_hypothetical_protein_184085..184315_NEGATIVE 212 60 16 51 6 NC_002950:PG0616|5end_thioredoxin,_putative_668935..669165_POSITIVE 207 120 84 36 2 NC_002950:PG2170|5end_sugar_transporter_2277974..2278204_NEGATIVE 202 44 3 47 12 NC_002950:PG0908|5end_G/U_mismatch-specific_DNA_glycosylase,_putative_971721..971951_NEGATIVE 201 31 1 166 127 NC_002950:PG1162|5end_ATP:cob(I)alamin_adenosyltransferase,_putative_1242039..1242269_NEGATIVE 197 45 14 41 6 NC_002950:PG0671|5end_iron_compound_ABC_transporter,_permease_protein_721888..722118_POSITIVE 197 44 1 212 156 NC_002950:PG0172|5end_exonuclease_193493..193723_POSITIVE 194 103 66 68 30 NC_002950:PG2031|5end_hypothetical_protein_2130339..2130569_POSITIVE 194 113 72 60 25 NC_002950:PG1230|5end_hypothetical_protein_1308068..1308298_NEGATIVE 194 42 2 85 35 NC_002950:PG0883|5end_hypothetical_protein_945121..945351_POSITIVE 193 68 31 164 121 NC_002950:PG0656|5end_50S_ribosomal_protein_L34_707097..707327_NEGATIVE 192 120 79 63 4 NC_002950:PG1184|5end_alginate_O-acetyltransferase,_putative_1266870..1267100_NEGATIVE 192 43 2 79 30 NC_002950:PG0026|5end_hypothetical_protein_29234..29464_POSITIVE 191 43 2 78 36 NC_002950:PG0465|5end_ferric_uptake_transcriptional_regulator_509403..509633_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 259 120 78 79 36 NC_002950:PG1304|3end_hypothetical_protein_1382134..1382284_NEGATIVE 216 30 1 54 26 NC_002950:PG0022|3end_sulfate_permease_family_protein_25120..25270_NEGATIVE 213 120 78 69 25 NC_002950:PG1828|3end_lipoprotein,_putative_1920924..1921074_NEGATIVE 209 45 2 149 104 NC_002950:PG1528|3end_hypothetical_protein_1607188..1607338_POSITIVE 208 46 2 135 99 NC_002950:PG0354|3end_hypothetical_protein_389131..389281_NEGATIVE 204 112 72 113 77 NC_002950:PG1814|3end_DNA_primase_1908606..1908756_NEGATIVE 204 43 3 128 77 NC_002950:PG1619|3end_biotin_synthesis_protein_BioC,_putative_1699415..1699565_POSITIVE 202 28 2 56 24 NC_002950:PG0424|3end_hypothetical_protein_463213..463363_POSITIVE 201 31 1 143 104 NC_002950:PG1163|3end_cobyrinic_acid_a,c-diamide_synthase_1242062..1242212_NEGATIVE 197 45 14 44 9 NC_002950:PG0670|3end_lipoprotein,_putative_721971..722121_POSITIVE 195 41 2 79 31 NC_002950:PG0958|3end_ribonuclease_BN,_putative_1018135..1018285_NEGATIVE 194 103 66 57 19 NC_002950:PG2030|3end_hypothetical_protein_2130408..2130558_POSITIVE 194 42 2 88 38 NC_002950:PG0882|3end_hypothetical_protein_945204..945354_POSITIVE 193 68 31 102 59 NC_002950:PG0657|3end_Maf-like_protein_707159..707309_NEGATIVE 192 46 2 75 36 NC_002950:PG0915|3end_hypothetical_protein_977057..977207_POSITIVE 189 39 7 144 106 NC_002950:PG1870|3end_methlytransferase,_UbiE/COQ5_family_1965868..1966018_NEGATIVE 187 47 1 134 93 NC_002950:PG2214|3end_hypothetical_protein_2325301..2325451_NEGATIVE 187 112 79 110 85 NC_002950:PG2086|3end_hypothetical_protein_2187070..2187220_POSITIVE 187 119 77 55 7 NC_002950:PG0468|3end_mannose-6-phosphate_isomerase,_class_I_510374..510524_NEGATIVE 185 44 2 120 70 NC_002950:PG0137|3end_aminoacyl-histidine_dipeptidase_160638..160788_POSITIVE