Origin IGS: cgagatgattattttcgatcggtatgctctaccaaccgatcgctttaatcggtcgcccggcttccataaaaaggagtcgggcgactcttttactccaaccaaataagcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccaagctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatcttcctgttcggaaacaatcgcagggctat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atagccctgcgattgtttccgaacaggaagatgggctataccacagggcagatggtggtactccgtagaggataacgggctatcccgtacggctcttcgagctacgctgtagagctcagcgaggtacgctctacagcgtagcgagccgagccgtagagttccgctccgggagccgtagaggtggccgagctgagccgtagagcttggcgcggtgagccctacagcgtagctcgaagagccgtagagcgcagctcggtacgctctacggcgtagcccgagtcgctgtagggcgtagctcgacccccttccggagtatattccgaacggctataaaacaatgcttatttggttggagtaaaagagtcgcccgactcctttttatggaagccgggcgaccgattaaagcgatcggttggtagagcataccgatcgaaaataatcatctcg Mask Tandem Repeat Region ================================================ cgagatgattattttcgatcggtatgctctaccaaccgatcgctttaatcggtcgcccggcttccataaaaaggagtcgggcgactcttttactccaaccaaataagcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaccgcgccaagctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatcttcctgttcggaaacaatcgcagggctat Find is-nt database================================================ Query_seq: PG1837:PG1840|PG1837:PG1840:hemagglutinin protein HagA:hypothetical protein:->->:1936795..1937241 447 cgagatgattattttcgatcggtatgctctaccaaccgatcgctttaatcggtcgcccggcttccataaaaaggagtcgggcgactcttttactccaaccaaataagcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccaagctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatcttcctgttcggaaacaatcgcagggctat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1837:PG1840|PG1837:PG1840:hemagglutinin protein HagA:hypothetical protein:->->:1936795..1937241 447 cgagatgattattttcgatcggtatgctctaccaaccgatcgctttaatcggtcgcccggcttccataaaaaggagtcgggcgactcttttactccaaccaaataagcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccaagctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatcttcctgttcggaaacaatcgcagggctat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1837:PG1840|PG1837:PG1840:hemagglutinin protein HagA:hypothetical protein:->->:1936795..1937241 447 cgagatgattattttcgatcggtatgctctaccaaccgatcgctttaatcggtcgcccggcttccataaaaaggagtcgggcgactcttttactccaaccaaataagcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccaagctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatcttcctgttcggaaacaatcgcagggctat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cgagatgattattttcgatcggtatgctctaccaaccgatcgctttaatcggtcgcccggcttccataaaaaggagtcgggcgactcttttactccaaccaaataagcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccaagctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatcttcctgttcggaaacaatcgcagggctat Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 5 End: 103 .... M I I F D R Y A L P T D R F N R S P G F H K K E S G D S F T P T K ........................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 55 Strand: + Start: 18 End: 182 ................. M G M L Y Q P I A L I G R P A S I K R S R A T L L L Q P N K H C F I A V R N I L R K G V E L R P T A T R A T P ......................................................................................................................................................................................................................................................................... Protein_Len: 39 Strand: + Start: 110 End: 226 ............................................................................................................. M F Y S R S E Y T P E G G R A T P Y S D S G Y A V E R T E L R S T A L R A T L ............................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 130 End: 447 ................................................................................................................................. M Y S G R G S S Y A L Q R L G L R R R A Y R A A L Y G S S S Y A V G L T A P S S T A Q L G H L Y G S R S G T L R L G S L Protein_Len: 42 Strand: - Start: 251 End: 376 .......................................................................................................................................................................................................................................................... P E A R G G R R S G S R F E V A R S A V S Y L T G R Q A R C R L E F L R V P Y G T M ....................................................................... Protein_Len: 30 Strand: - Start: 178 End: 267 ................................................................................................................................................................................. A T S R V S S R E V A R R A V S Y P E G R W A R R S L E A M .................................................................................................................................................................................... Protein_Len: 34 Strand: - Start: 32 End: 133 ............................... W G I A K I P R G A E M F L L R A V R K S W G F L C Q K I A T R F M .......................................................................................................................................................................................................................................................................................................................... Protein_Len: 34 Strand: - Start: 1 End: 102 S I I I K S R Y A R G V S R K L R D G P K W L F S D P S E K V G V M ......................................................................................................................................................................................................................................................................................................................................................... cgagatgattattttcgatcggtatgctctaccaaccgatcgctttaatcggtcgcccggcttccataaaaaggagtcgggcgactcttttactccaaccaaataagcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccaagctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatcttcctgttcggaaacaatcgcagggctat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cgagatgattattttcgatcggtatgctctaccaaccgatcgctttaatcggtcgcccggcttccataaaaaggagtcgggcgactcttttactccaaccaaataagcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccaagctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatcttcctgttcggaaacaatcgcagggctat Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 82 HP_score: -10.2 Tail_Score: -4.25104 Start: 51 End: 87 Full_Region: CCGATCGCTTTAATC GGTCGCCCGGCTTCCAT AAA AAGGAGTCGGGCGACTC TTTTACTCCAACCAA ..................................................GGTCGCCCGGCTTCCATAAAAAGGAGTCGGGCGACTC........................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 88 HP_score: -13.9 Tail_Score: -3.73042 Start: 52 End: 85 Full_Region: CGATCGCTTTAATCG GTCGCCCGGCTTCCAT AAA AAGGAGTCGGGCGAC TCTTTTACTCCAACC ...................................................GTCGCCCGGCTTCCATAAAAAGGAGTCGGGCGAC.......................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 73 HP_score: -3.2 Tail_Score: -4.82901 Start: 73 End: 88 Full_Region: tttggttggagtaaa agagtc gccc gactcc tttttatggaagccg ........................................................................agagtcgcccgactcc....................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 89 HP_score: -16.7 Tail_Score: -3.04263 Start: 179 End: 210 Full_Region: AGCGACTCGGGCTAC GCCGTAGAGCGTA CCGAGC TGCGCTCTACGGC TCTTCGAGCTACGCT ..................................................................................................................................................................................GCCGTAGAGCGTACCGAGCTGCGCTCTACGGC............................................................................................................................................................................................................................................. Find igs database================================================ Query_seq: PG1837:PG1840|PG1837:PG1840:hemagglutinin protein HagA:hypothetical protein:->->:1936795..1937241 447 cgagatgattattttcgatcggtatgctctaccaaccgatcgctttaatcggtcgcccggcttccataaaaaggagtcgggcgactcttttactccaaccaaataagcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccaagctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatcttcctgttcggaaacaatcgcagggctat Intra-Species Hit: Count: 7 Min: 1 Max: 447 Len: 447 Subject: NC_002950_PG1837_PG1840|hemagglutinin protein HagA:hypothetical protein|POSITIVE:POSITIVE|[1936795,1937241]|447 HSP 1 e-value: 0.0 bit: 886.0 Len: 447 Query Start:1 Query End:447 Subject Strand: POSITIVE Subject Start: 1 Subject End: 447 cgagatgattattttcgatcggtatgctctaccaaccgatcgctttaatcggtcgcccggcttccataaaaaggagtcgggcgactcttttactccaaccaaataagcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccaagctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatcttcctgttcggaaacaatcgcagggctat cgagatgattattttcgatcggtatgctctaccaaccgatcgctttaatcggtcgcccggcttccataaaaaggagtcgggcgactcttttactccaaccaaataagcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccaagctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatcttcctgttcggaaacaatcgcagggctat HSP 2 e-value: 4.0E-6 bit: 54.0 Len: 31 Query Start:330 Query End:360 Subject Strand: NEGATIVE Subject Start: 205 Subject End: 235 .........................................................................................................................................................................................................................................................................................................................................tgagctctacagcgtagctcgaagagccgta....................................................................................... .........................................................................................................................................................................................................................................................................................................................................tgagccctacagcgtagctcgaagagccgta....................................................................................... HSP 3 e-value: 4.0E-6 bit: 54.0 Len: 31 Query Start:205 Query End:235 Subject Strand: NEGATIVE Subject Start: 330 Subject End: 360 ............................................................................................................................................................................................................tacggctcttcgagctacgctgtagggctca.................................................................................................................................................................................................................... ............................................................................................................................................................................................................tacggctcttcgagctacgctgtagagctca.................................................................................................................................................................................................................... Subject: NC_002950_PG1190_PG1195|glycerate dehydrogenase:8-amino-7-oxononanoate synthase|POSITIVE:POSITIVE|[1273206,1274118]|913 HSP 1 e-value: 1.0E-143 bit: 509.0 Len: 313 Query Start:107 Query End:419 Subject Strand: POSITIVE Subject Start: 296 Subject End: 608 ..........................................................................................................gcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccaagctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatct............................ ..........................................................................................................gcattgttttatagccgttcggaacatatgccgaaggggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccgggccctacggctcagctcggccacctctacggctcacggagcggaactctacggctcggctcgctacgctgtagggcgtacctcgccgagctctacagcgtagctcgaagagccgtacgggatagctcgtcatcctctacggagtaccgccatctgccctgtggtatagcccatct............................ HSP 2 e-value: 4.0E-6 bit: 54.0 Len: 31 Query Start:330 Query End:360 Subject Strand: NEGATIVE Subject Start: 394 Subject End: 424 .........................................................................................................................................................................................................................................................................................................................................tgagctctacagcgtagctcgaagagccgta....................................................................................... .........................................................................................................................................................................................................................................................................................................................................tgagccctacagcgtagctcgaagagccgta....................................................................................... Subject: NC_002950_PG0890_PG0893|alkaline phosphatase, putative:hydroxylamine reductase|POSITIVE:NEGATIVE|[953164,954290]|1127 HSP 1 e-value: 1.0E-142 bit: 507.0 Len: 316 Query Start:107 Query End:422 Subject Strand: NEGATIVE Subject Start: 375 Subject End: 690 ..........................................................................................................gcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccaagctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatcttcc......................... ..........................................................................................................gcattgttttatagccattcggaacatacgccgaaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcaagctacgctgtagggctcaccgcgccgggccctacggctcagctcggccacatctacggctcacggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgccgagctctacagcgtagctcgaagagccgtatgggatagcccgttatcctctacggaataccgccatctgccttgtggtatagcccatcttcc......................... Subject: NC_002950_PG1881_PG1884|hypothetical protein:alpha-L-fucosidase precursor, putative|POSITIVE:NEGATIVE|[1978107,1979057]|951 HSP 1 e-value: 1.0E-138 bit: 492.0 Len: 324 Query Start:107 Query End:430 Subject Strand: NEGATIVE Subject Start: 366 Subject End: 689 ..........................................................................................................gcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccaagctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatcttcctgttcgga................. ..........................................................................................................gcattgttttatagccgttcggaacatactccgaaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcggcgcgccgggctctagggctcggctcggccacctctacagctcccggagcggaactctacggctcggctcgctacgctgtagggcgtacctcgctgagctctacagcgtagctcgaagagctgtacgggatagctcgtcatcccctacggagtacctccatgtgccctatggtatagcccatctttctgtacgga................. Subject: NC_002950_PG1297_PG1300|ribosomal protein S1:hypothetical protein|POSITIVE:NEGATIVE|[1377771,1378619]|849 HSP 1 e-value: 1.0E-138 bit: 492.0 Len: 323 Query Start:108 Query End:430 Subject Strand: NEGATIVE Subject Start: 302 Subject End: 625 ...........................................................................................................cattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccaagctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatc-tgccctgtggtatagcccatcttcctgttcgga................. ...........................................................................................................cattgttttatagccgttcggaatatactccgaaggggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcgctgcgccgggctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagggctcaccgcgccgggctctacagcgtagctcgaagagctgtacgggatagcccgttatcctctatggagtaccgccatcttgccctgtggtatagcccatctccctgtacgga................. Subject: NC_002950_PG0237_PG0240|uracil-DNA glycosylase:hydrolase, haloacid dehalogenase-like family|POSITIVE:NEGATIVE|[275587,276484]|898 HSP 1 e-value: 1.0E-86 bit: 321.0 Len: 186 Query Start:245 Query End:430 Subject Strand: NEGATIVE Subject Start: 274 Subject End: 459 ....................................................................................................................................................................................................................................................gctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatcttcctgttcgga................. ....................................................................................................................................................................................................................................................gctctacggctcagctcggccacctctacggctcccggagcggaactctacggctcggctcgctacgctgtagagcgtacctcgccgagctctacagcgtagctcgaagagctgtacgggatagcccgttatcctctacggtgtaccgccatctgccttgtggtatagcccatcttcctgtacgga................. HSP 2 e-value: 1.0E-43 bit: 178.0 Len: 106 Query Start:107 Query End:212 Subject Strand: NEGATIVE Subject Start: 448 Subject End: 553 ..........................................................................................................gcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctc........................................................................................................................................................................................................................................... ..........................................................................................................gcattgttttatagccattcggaacatacgccgaaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctc........................................................................................................................................................................................................................................... Subject: NC_002950_PG1954_PG1956|NAD dependent epimerase/reductase-related protein:4-hydroxybutyrate CoA-transferase|POSITIVE:NEGATIVE|[2045673,2046304]|632 HSP 1 e-value: 2.0E-57 bit: 224.0 Len: 145 Query Start:107 Query End:251 Subject Strand: NEGATIVE Subject Start: 215 Subject End: 359 ..........................................................................................................gcattgttttatagccgttcggaatatactccggaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagggctcaccgcgccaagctctac.................................................................................................................................................................................................... ..........................................................................................................gcattgttttatagccgttcggaacatacgccgaaagggggtcgagctacgccctacagcgactcgggctacgccgtagagcgtaccgagctgcgctctacggctcttcgagctacgctgtagagcgtacctcgccgagctctac.................................................................................................................................................................................................... HSP 2 e-value: 4.0E-43 bit: 176.0 Len: 125 Query Start:306 Query End:430 Subject Strand: NEGATIVE Subject Start: 124 Subject End: 248 .................................................................................................................................................................................................................................................................................................................gctacgctgtagagcgtacctcgctgagctctacagcgtagctcgaagagccgtacgggatagcccgttatcctctacggagtaccaccatctgccctgtggtatagcccatcttcctgttcgga................. .................................................................................................................................................................................................................................................................................................................gctacgctgtagagcgtacctcgccgagctctacagcgtagctcgaagagccgtacgggatagctcgtcatcctctatggtgtaccgccatctgccctatggtatagcccatctttctgtacgga................. HSP 3 e-value: 1.0E-6 bit: 56.0 Len: 28 Query Start:333 Query End:360 Subject Strand: POSITIVE Subject Start: 234 Subject End: 261 ............................................................................................................................................................................................................................................................................................................................................gctctacagcgtagctcgaagagccgta....................................................................................... ............................................................................................................................................................................................................................................................................................................................................gctctacagcgtagctcgaagagccgta....................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGAGAUGAUUAUUUUCGAUCGGUAUGCUCUACCAACCGAUCGCUUUAAUCGGUCGCCCGGCUUCCAUAAAAAGGAGUCGGGCGACUCUUUUACUCCAACCAAAUAAGCAUUGUUUUAUAGCCGUUCGGAAUAUACUCCGGAAGGGGGUCGAGCUACGCCCUACAGCGACUCGGGCUACGCCGUAGAGCGUACCGAGCUGCGCUCUACGGCUCUUCGAGCUACGCUGUAGGGCUCACCGCGCCAAGCUCUACGGCUCAGCUCGGCCACCUCUACGGCUCCCGGAGCGGAACUCUACGGCUCGGCUCGCUACGCUGUAGAGCGUACCUCGCUGAGCUCUACAGCGUAGCUCGAAGAGCCGUACGGGAUAGCCCGUUAUCCUCUACGGAGUACCACCAUCUGCCCUGUGGUAUAGCCCAUCUUCCUGUUCGGAAACAAUCGCAGGGCUAU .(((..(((((....((((((((.((......))))))))))...))))).(((((((((((((........))))))))))))).......)))...........................((((((......((((....)))).))))))...(((((...((((...(((((...((((((((.(((.((.((((..((((((((((((((((((((((((((((((((((.((.(....((((((((((..(((..((((........)))).(((.(((.(.......).))))))...)))..))))))))))....).)).))))))))))))))))))))))))))))))).))).)))).)).))).)))))))).(((((((..........))))))))))))...((((.....))))....)))))))))... (-215.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAGCCCUGCGAUUGUUUCCGAACAGGAAGAUGGGCUAUACCACAGGGCAGAUGGUGGUACUCCGUAGAGGAUAACGGGCUAUCCCGUACGGCUCUUCGAGCUACGCUGUAGAGCUCAGCGAGGUACGCUCUACAGCGUAGCGAGCCGAGCCGUAGAGUUCCGCUCCGGGAGCCGUAGAGGUGGCCGAGCUGAGCCGUAGAGCUUGGCGCGGUGAGCCCUACAGCGUAGCUCGAAGAGCCGUAGAGCGCAGCUCGGUACGCUCUACGGCGUAGCCCGAGUCGCUGUAGGGCGUAGCUCGACCCCCUUCCGGAGUAUAUUCCGAACGGCUAUAAAACAAUGCUUAUUUGGUUGGAGUAAAAGAGUCGCCCGACUCCUUUUUAUGGAAGCCGGGCGACCGAUUAAAGCGAUCGGUUGGUAGAGCAUACCGAUCGAAAAUAAUCAUCUCG ...((((((......(((((.....)))))..((......)).))))))...(((..((((((((..((((....(((((((((((.(((((((((((((((((((((((((.(((((.((.(.((((((....)))))).)..((((((((...(.((((.((((.((..(((.....)))..)))))).)))))...).))))))).)).))))).))))))))))))))))))))))))).((((..((((((((((((....))))))..))))))))))...))).))))))))....)))).))))))))...)))..((((((................))))))(((.......((((((((.((((.......)).)).)))))))).(((((...((((((((((......)).))))))))....)))))..))). (-220.49) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 560 189 142 107 49 NC_002950:PG1006|5end_hypothetical_protein_1066478..1066708_NEGATIVE 512 308 261 174 123 NC_002950:PG1850|5end_hypothetical_protein_1949957..1950187_NEGATIVE 506 269 221 172 123 NC_002950:PG2199|5end_ABC_transporter,_ATP-binding_protein,_putative_2306723..2306953_NEGATIVE 489 277 231 171 119 NC_002950:PG1966|5end_hypothetical_protein_2056924..2057154_NEGATIVE 487 197 152 72 14 NC_002950:PG1343|5end_lipoate-protein_ligase_B_1423628..1423858_POSITIVE 483 297 253 151 93 NC_002950:PG0809|5end_hypothetical_protein_872014..872244_NEGATIVE 476 270 230 76 27 NC_002950:PG0606|5end_hypothetical_protein_662558..662788_NEGATIVE 476 270 230 228 179 NC_002950:PG0605|5end_hypothetical_protein_662406..662636_NEGATIVE 474 168 121 217 158 NC_002950:PG2068|5end_glycerol-3-phosphate_cytidylyltransferase_2164334..2164564_POSITIVE 466 240 191 71 24 NC_002950:PG0593|5end_htrA_protein_650362..650592_POSITIVE 465 213 171 131 75 NC_002950:PG0458|5end_ISPg5,_transposase_Orf2_501463..501693_NEGATIVE 460 240 191 68 11 NC_002950:PG1992|5end_glucose-inhibited_division_protein_A_2081410..2081640_POSITIVE 460 277 231 52 10 NC_002950:PG0714|5end_copper_homeostasis_protein_CutC_768671..768901_NEGATIVE 459 330 281 198 133 NC_002950:PG0215|5end_hypothetical_protein_251460..251690_POSITIVE 459 286 246 160 114 NC_002950:PG0203|5end_hypothetical_protein_240211..240441_NEGATIVE 456 310 261 206 142 NC_002950:PG1306|5end_metallo-beta-lactamase_family_protein_1387187..1387417_NEGATIVE 455 307 261 222 170 NC_002950:PG1162|5end_ATP:cob(I)alamin_adenosyltransferase,_putative_1242039..1242269_NEGATIVE 454 320 273 77 31 NC_002950:PG2038|5end_N-acetylmuramoyl-L-alanine_amidase,_putative_2138207..2138437_NEGATIVE 453 190 144 223 167 NC_002950:PG0219|5end_hypothetical_protein_255558..255788_NEGATIVE 452 259 211 159 113 NC_002950:PG0955|5end_hypothetical_protein_1014832..1015062_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 743 237 193 70 17 NC_002950:PG0261|3end_ISPg3,_transposase_293862..294012_NEGATIVE 560 189 142 67 9 NC_002950:PG1007|3end_transcriptional_regulator,_GntR_family_1066518..1066668_NEGATIVE 479 310 261 49 5 NC_002950:PG1247|3end_3-dehydroquinate_synthase_1325585..1325735_POSITIVE 465 213 171 77 21 NC_002950:PG0459|3end_ISPg5,_transposase_Orf1_501517..501667_NEGATIVE 460 249 201 62 6 NC_002950:PG1133|3end_hypothetical_protein_1214103..1214253_POSITIVE 460 277 231 47 5 NC_002950:PG0715|3end_transporter_768676..768826_NEGATIVE 459 286 246 109 63 NC_002950:PG0204|3end_hypothetical_protein_240262..240412_NEGATIVE 456 279 232 60 16 NC_002950:PG0605|3end_hypothetical_protein_661165..661315_NEGATIVE 454 339 294 128 87 NC_002950:PG1675|3end_hypothetical_protein_1760360..1760510_POSITIVE 449 268 222 144 99 NC_002950:PG0361|3end_hypothetical_protein_395481..395631_POSITIVE 445 330 282 128 60 NC_002950:PG2101|3end_hypothetical_protein_2210278..2210428_NEGATIVE 440 288 244 136 74 NC_002950:PG0320|3end_hypothetical_protein_354577..354727_POSITIVE 440 215 171 67 23 NC_002950:PG0211|3end_cobalamin_biosynthesis_protein_CbiG/precorrin-4_C11-methyltransferase_245966..246116_NEGATIVE 438 320 271 136 65 NC_002950:PG1353|3end_orotate_phosphoribosyltransferase_1433201..1433351_NEGATIVE 436 189 142 71 15 NC_002950:PG1411|3end_potassium_uptake_protein_TrkA,_putative_1493481..1493631_NEGATIVE 434 209 163 107 51 NC_002950:PG2094|3end_hypothetical_protein_2193321..2193471_NEGATIVE 432 310 262 61 11 NC_002950:PG0803|3end_glucosamine-6-phosphate_deaminase_859727..859877_NEGATIVE 431 215 171 147 101 NC_002950:PG1480|3end_conjugative_transposon_protein_TraI_1555266..1555416_NEGATIVE 430 85 51 39 4 NC_002950:PG1837|3end_hemagglutinin_protein_HagA_1936734..1936884_POSITIVE 424 308 264 139 96 NC_002950:PG0377|3end_30S_ribosomal_protein_S2_407845..407995_POSITIVE