Origin IGS: aggaggaagaaagtcttcttcctctctcccgatctcatcactctgctcatggtcgtacacttccccgtgcaaataacgatcgggaacctctttcatatcggtggagaggggatagagacgttcacccggtgacaagtgttgcagctcttcgctcatctttgttctcgttctgtattctatatgattctctgcattccaaattcagacagcctgtggactaaccccggataataatccggtgcagcgtgtgaaaatcatacacgaaagtacgaataatacgcccaaccgtctgtctcgaacctcagccgatctccgacaattccggaaccgcaaaagctgcgattgcggttcgtgtttttgggaaaagtgacgcgagatttttttcgttgtggcgcgtaaatttttcttttctcgcaccaaaaggaaaacttttacgcgccgcatttttagggccggtgagtcgccgttttttggctcgtgaaaaggtggcgatccccagccggttcttcttcggctatttcttttcccatcttgtcggatcttttggcttaatcctctcataatcaggtgctcatccttgagtggtttgtctctcgattcgcactttggtctcctccgtttaggtctggttttgggagcttgtttatggtagcctttgcagtgcgattcttgaggtatttggtctttttatacttatctaagagggctttacgtacttcttccttatcttgccgatgagttttttttgtacctttgcaagaaacgtagatgacggtaaaaga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tcttttaccgtcatctacgtttcttgcaaaggtacaaaaaaaactcatcggcaagataaggaagaagtacgtaaagccctcttagataagtataaaaagaccaaatacctcaagaatcgcactgcaaaggctaccataaacaagctcccaaaaccagacctaaacggaggagaccaaagtgcgaatcgagagacaaaccactcaaggatgagcacctgattatgagaggattaagccaaaagatccgacaagatgggaaaagaaatagccgaagaagaaccggctggggatcgccaccttttcacgagccaaaaaacggcgactcaccggccctaaaaatgcggcgcgtaaaagttttccttttggtgcgagaaaagaaaaatttacgcgccacaacgaaaaaaatctcgcgtcacttttcccaaaaacacgaaccgcaatcgcagcttttgcggttccggaattgtcggagatcggctgaggttcgagacagacggttgggcgtattattcgtactttcgtgtatgattttcacacgctgcaccggattattatccggggttagtccacaggctgtctgaatttggaatgcagagaatcatatagaatacagaacgagaacaaagatgagcgaagagctgcaacacttgtcaccgggtgaacgtctctatcccctctccaccgatatgaaagaggttcccgatcgttatttgcacggggaagtgtacgaccatgagcagagtgatgagatcgggagagaggaagaagactttcttcctcct Mask Tandem Repeat Region ================================================ aggaggaagaaagtcttcttcctctctcccgatctcatcactctgctcatggtcgtacacttccccgtgcaaataacgatcgggaacctctttcatatcggtggagaggggatagagacgttcacccggtgacaagtgttgcagctcttcgctcatctttgttctcgttctgtattctatatgattctctgcattccaaattcagacagcctgtggactaaccccggataataatccggtgcagcgtgtgaaaatcatacacgaaagtacgaataatacgcccaaccgtctgtctcgaacctcagccgatctccgacaattccggaaccgcaaaagctgcgattgcggttcgtgtttttgggaaaagtgacgcgagatttttttcgttgtggcgcgtaaatttttcttttctcgcaccaaaaggaaaacttttacgcgccgcatttttagggccggtgagtcgccgttttttggctcgtgaaaaggtggcgatccccagccggttcttcttcggctatttcttttcccatcttgtcggatcttttggcttaatcctctcataatcaggtgctcatccttgagtggtttgtctctcgattcgcactttggtctcctccgtttaggtctggttttgggagcttgtttatggtagcctttgcagtgcgattcttgaggtatttggtctttttatacttatctaagagggctttacgtacttcttccttatcttgccgatgagttttttttgtacctttgcaagaaacgtagatgacggtaaaaga Find is-nt database================================================ Query_seq: PG1831:PG1834|PG1831:PG1834:ATP-dependent DNA helicase RecQ:glycogen synthase-related protein:<-->:1925513..1926294 782 aggaggaagaaagtcttcttcctctctcccgatctcatcactctgctcatggtcgtacacttccccgtgcaaataacgatcgggaacctctttcatatcggtggagaggggatagagacgttcacccggtgacaagtgttgcagctcttcgctcatctttgttctcgttctgtattctatatgattctctgcattccaaattcagacagcctgtggactaaccccggataataatccggtgcagcgtgtgaaaatcatacacgaaagtacgaataatacgcccaaccgtctgtctcgaacctcagccgatctccgacaattccggaaccgcaaaagctgcgattgcggttcgtgtttttgggaaaagtgacgcgagatttttttcgttgtggcgcgtaaatttttcttttctcgcaccaaaaggaaaacttttacgcgccgcatttttagggccggtgagtcgccgttttttggctcgtgaaaaggtggcgatccccagccggttcttcttcggctatttcttttcccatcttgtcggatcttttggcttaatcctctcataatcaggtgctcatccttgagtggtttgtctctcgattcgcactttggtctcctccgtttaggtctggttttgggagcttgtttatggtagcctttgcagtgcgattcttgaggtatttggtctttttatacttatctaagagggctttacgtacttcttccttatcttgccgatgagttttttttgtacctttgcaagaaacgtagatgacggtaaaaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1831:PG1834|PG1831:PG1834:ATP-dependent DNA helicase RecQ:glycogen synthase-related protein:<-->:1925513..1926294 782 aggaggaagaaagtcttcttcctctctcccgatctcatcactctgctcatggtcgtacacttccccgtgcaaataacgatcgggaacctctttcatatcggtggagaggggatagagacgttcacccggtgacaagtgttgcagctcttcgctcatctttgttctcgttctgtattctatatgattctctgcattccaaattcagacagcctgtggactaaccccggataataatccggtgcagcgtgtgaaaatcatacacgaaagtacgaataatacgcccaaccgtctgtctcgaacctcagccgatctccgacaattccggaaccgcaaaagctgcgattgcggttcgtgtttttgggaaaagtgacgcgagatttttttcgttgtggcgcgtaaatttttcttttctcgcaccaaaaggaaaacttttacgcgccgcatttttagggccggtgagtcgccgttttttggctcgtgaaaaggtggcgatccccagccggttcttcttcggctatttcttttcccatcttgtcggatcttttggcttaatcctctcataatcaggtgctcatccttgagtggtttgtctctcgattcgcactttggtctcctccgtttaggtctggttttgggagcttgtttatggtagcctttgcagtgcgattcttgaggtatttggtctttttatacttatctaagagggctttacgtacttcttccttatcttgccgatgagttttttttgtacctttgcaagaaacgtagatgacggtaaaaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1831:PG1834|PG1831:PG1834:ATP-dependent DNA helicase RecQ:glycogen synthase-related protein:<-->:1925513..1926294 782 aggaggaagaaagtcttcttcctctctcccgatctcatcactctgctcatggtcgtacacttccccgtgcaaataacgatcgggaacctctttcatatcggtggagaggggatagagacgttcacccggtgacaagtgttgcagctcttcgctcatctttgttctcgttctgtattctatatgattctctgcattccaaattcagacagcctgtggactaaccccggataataatccggtgcagcgtgtgaaaatcatacacgaaagtacgaataatacgcccaaccgtctgtctcgaacctcagccgatctccgacaattccggaaccgcaaaagctgcgattgcggttcgtgtttttgggaaaagtgacgcgagatttttttcgttgtggcgcgtaaatttttcttttctcgcaccaaaaggaaaacttttacgcgccgcatttttagggccggtgagtcgccgttttttggctcgtgaaaaggtggcgatccccagccggttcttcttcggctatttcttttcccatcttgtcggatcttttggcttaatcctctcataatcaggtgctcatccttgagtggtttgtctctcgattcgcactttggtctcctccgtttaggtctggttttgggagcttgtttatggtagcctttgcagtgcgattcttgaggtatttggtctttttatacttatctaagagggctttacgtacttcttccttatcttgccgatgagttttttttgtacctttgcaagaaacgtagatgacggtaaaaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aggaggaagaaagtcttcttcctctctcccgatctcatcactctgctcatggtcgtacacttccccgtgcaaataacgatcgggaacctctttcatatcggtggagaggggatagagacgttcacccggtgacaagtgttgcagctcttcgctcatctttgttctcgttctgtattctatatgattctctgcattccaaattcagacagcctgtggactaaccccggataataatccggtgcagcgtgtgaaaatcatacacgaaagtacgaataatacgcccaaccgtctgtctcgaacctcagccgatctccgacaattccggaaccgcaaaagctgcgattgcggttcgtgtttttgggaaaagtgacgcgagatttttttcgttgtggcgcgtaaatttttcttttctcgcaccaaaaggaaaacttttacgcgccgcatttttagggccggtgagtcgccgttttttggctcgtgaaaaggtggcgatccccagccggttcttcttcggctatttcttttcccatcttgtcggatcttttggcttaatcctctcataatcaggtgctcatccttgagtggtttgtctctcgattcgcactttggtctcctccgtttaggtctggttttgggagcttgtttatggtagcctttgcagtgcgattcttgaggtatttggtctttttatacttatctaagagggctttacgtacttcttccttatcttgccgatgagttttttttgtacctttgcaagaaacgtagatgacggtaaaaga Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 37 End: 129 .................................... M T L L M V V H F P V Q I T I G N L F H I G G E G I E T F T R ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 30 Strand: + Start: 129 End: 218 ................................................................................................................................ M T S V A A L R S S L F S F C I L Y D S L H S K F R Q P V D .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: + Start: 136 End: 228 ....................................................................................................................................... M L Q L F A H L C S R S V F Y M I L C I P N S D S L W T N P G .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 56 Strand: + Start: 200 End: 367 ....................................................................................................................................................................................................... M Q T A C G L T P D N N P V Q R V K I I H E S T N N T P N R L S R T S A D L R Q F R N R K S C D C G S C F W E K ............................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 34 Strand: + Start: 377 End: 478 ........................................................................................................................................................................................................................................................................................................................................................................................ M F F V V A R K F F F S R T K R K T F T R R I F R A G E S P F F G S ................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 273 End: 560 ................................................................................................................................................................................................................................................................................ M I R P T V C L E P Q P I S D N S G T A K A A I A V R V F G K S D A R F F S L W R V N F S F L A P K G K L L R A A F L G .............................................................................................................................................................................................................................. Protein_Len: 37 Strand: + Start: 539 End: 649 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M F W L N P L I I R C S S L S G L S L D S H F G L L R L G L V L G A C L W ..................................................................................................................................... Protein_Len: 56 Strand: + Start: 568 End: 735 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M L I L E W F V S R F A L W S P P F R S G F G S L F M V A F A V R F L R Y L V F L Y L S K R A L R T S S L S C R ............................................... Protein_Len: 31 Strand: + Start: 677 End: 769 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M W S F Y T Y L R G L Y V L L P Y L A D E F F L Y L C K K R R ............. Protein_Len: 60 Strand: - Start: 567 End: 770 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E R N A S Q D G G N L D P K P L K N I T A K A T R N K L Y K T K K Y K D L L A K R V E E K D Q R H T K K T G K C S V Y M ............ Protein_Len: 60 Strand: - Start: 305 End: 499 ................................................................................................................................................................................................................................................................................................................ E P V A F A A I A T R T K P F L S A L N K E N H R T F K E K R A G F P F S K R A A N K P G T L R R K K A R S F T A I G M ........................................................................................................................................................................................................................................................................................... Protein_Len: 48 Strand: - Start: 258 End: 401 ................................................................................................................................................................................................................................................................. V R F Y S Y Y A W G D T E F R L R D G V I G S G C F S R N R N T N K P F T V R S K K R Q P A Y M ............................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 205 End: 678 ............................................................................................................................................................................................................ G T R R R S N R K G D Q R I K Q S L G R M I L H E D K L P K D R S E C K P R R R K P R T K P A Q K H Y G K C H S E Q P M ........................................................................................................ Protein_Len: 60 Strand: - Start: 1 End: 201 E R G I E D S Q E H D Y V E G H L Y R D P V E K M D T S L P Y L R E G P S L H Q L E E S M K T R T R Y E I H N E A N W M ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... aggaggaagaaagtcttcttcctctctcccgatctcatcactctgctcatggtcgtacacttccccgtgcaaataacgatcgggaacctctttcatatcggtggagaggggatagagacgttcacccggtgacaagtgttgcagctcttcgctcatctttgttctcgttctgtattctatatgattctctgcattccaaattcagacagcctgtggactaaccccggataataatccggtgcagcgtgtgaaaatcatacacgaaagtacgaataatacgcccaaccgtctgtctcgaacctcagccgatctccgacaattccggaaccgcaaaagctgcgattgcggttcgtgtttttgggaaaagtgacgcgagatttttttcgttgtggcgcgtaaatttttcttttctcgcaccaaaaggaaaacttttacgcgccgcatttttagggccggtgagtcgccgttttttggctcgtgaaaaggtggcgatccccagccggttcttcttcggctatttcttttcccatcttgtcggatcttttggcttaatcctctcataatcaggtgctcatccttgagtggtttgtctctcgattcgcactttggtctcctccgtttaggtctggttttgggagcttgtttatggtagcctttgcagtgcgattcttgaggtatttggtctttttatacttatctaagagggctttacgtacttcttccttatcttgccgatgagttttttttgtacctttgcaagaaacgtagatgacggtaaaaga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aggaggaagaaagtcttcttcctctctcccgatctcatcactctgctcatggtcgtacacttccccgtgcaaataacgatcgggaacctctttcatatcggtggagaggggatagagacgttcacccggtgacaagtgttgcagctcttcgctcatctttgttctcgttctgtattctatatgattctctgcattccaaattcagacagcctgtggactaaccccggataataatccggtgcagcgtgtgaaaatcatacacgaaagtacgaataatacgcccaaccgtctgtctcgaacctcagccgatctccgacaattccggaaccgcaaaagctgcgattgcggttcgtgtttttgggaaaagtgacgcgagatttttttcgttgtggcgcgtaaatttttcttttctcgcaccaaaaggaaaacttttacgcgccgcatttttagggccggtgagtcgccgttttttggctcgtgaaaaggtggcgatccccagccggttcttcttcggctatttcttttcccatcttgtcggatcttttggcttaatcctctcataatcaggtgctcatccttgagtggtttgtctctcgattcgcactttggtctcctccgtttaggtctggttttgggagcttgtttatggtagcctttgcagtgcgattcttgaggtatttggtctttttatacttatctaagagggctttacgtacttcttccttatcttgccgatgagttttttttgtacctttgcaagaaacgtagatgacggtaaaaga Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 61 HP_score: -6.5 Tail_Score: -3.60925 Start: 322 End: 348 Full_Region: CGATCTCCGACAATT CCGGAACCGCA AAAGC TGCGATTGCGG TTCGTGTTTTTGGGA .................................................................................................................................................................................................................................................................................................................................CCGGAACCGCAAAAGCTGCGATTGCGG.................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 72 HP_score: -4.7 Tail_Score: -4.57073 Start: 322 End: 354 Full_Region: CGATCTCCGACAATT CCGGAACCGCAAAAG CTG CGATTGCGGTTCGTG TTTTTGGGAAAAGTG .................................................................................................................................................................................................................................................................................................................................CCGGAACCGCAAAAGCTGCGATTGCGGTTCGTG............................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 93 HP_score: -10.1 Tail_Score: -4.57073 Start: 388 End: 443 Full_Region: CGAGATTTTTTTCGT TGTGGCGCGTAAATTTTTCTTTT CTCGCACCA AAAGGAAAACTTTTACGCGCCGCA TTTTTAGGGCCGGTG ...................................................................................................................................................................................................................................................................................................................................................................................................TGTGGCGCGTAAATTTTTCTTTTCTCGCACCAAAAGGAAAACTTTTACGCGCCGCA................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 87 HP_score: -6.9 Tail_Score: -5.1101 Start: 454 End: 466 Full_Region: CCGCATTTTTAGGGC CGGTG AGT CGCCG TTTTTTGGCTCGTGA .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................CGGTGAGTCGCCG............................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 95 HP_score: -3.0 Tail_Score: -5.97575 Start: 723 End: 739 Full_Region: TTTACGTACTTCTTC CTTATC TTGCC GATGAG TTTTTTTTGTACCTT ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CTTATCTTGCCGATGAG........................................... Find igs database================================================ Query_seq: PG1831:PG1834|PG1831:PG1834:ATP-dependent DNA helicase RecQ:glycogen synthase-related protein:<-->:1925513..1926294 782 aggaggaagaaagtcttcttcctctctcccgatctcatcactctgctcatggtcgtacacttccccgtgcaaataacgatcgggaacctctttcatatcggtggagaggggatagagacgttcacccggtgacaagtgttgcagctcttcgctcatctttgttctcgttctgtattctatatgattctctgcattccaaattcagacagcctgtggactaaccccggataataatccggtgcagcgtgtgaaaatcatacacgaaagtacgaataatacgcccaaccgtctgtctcgaacctcagccgatctccgacaattccggaaccgcaaaagctgcgattgcggttcgtgtttttgggaaaagtgacgcgagatttttttcgttgtggcgcgtaaatttttcttttctcgcaccaaaaggaaaacttttacgcgccgcatttttagggccggtgagtcgccgttttttggctcgtgaaaaggtggcgatccccagccggttcttcttcggctatttcttttcccatcttgtcggatcttttggcttaatcctctcataatcaggtgctcatccttgagtggtttgtctctcgattcgcactttggtctcctccgtttaggtctggttttgggagcttgtttatggtagcctttgcagtgcgattcttgaggtatttggtctttttatacttatctaagagggctttacgtacttcttccttatcttgccgatgagttttttttgtacctttgcaagaaacgtagatgacggtaaaaga Intra-Species Hit: Count: 1 Min: 1 Max: 782 Len: 782 Subject: NC_002950_PG1831_PG1834|ATP-dependent DNA helicase RecQ:glycogen synthase-related protein|NEGATIVE:POSITIVE|[1925513,1926294]|782 HSP 1 e-value: 0.0 bit: 1461.0 Len: 782 Query Start:1 Query End:782 Subject Strand: POSITIVE Subject Start: 1 Subject End: 782 aggaggaagaaagtcttcttcctctctcccgatctcatcactctgctcatggtcgtacacttccccgtgcaaataacgatcgggaacctctttcatatcggtggagaggggatagagacgttcacccggtgacaagtgttgcagctcttcgctcatctttgttctcgttctgtattctatatgattctctgcattccaaattcagacagcctgtggactaaccccggataataatccggtgcagcgtgtgaaaatcatacacgaaagtacgaataatacgcccaaccgtctgtctcgaacctcagccgatctccgacaattccggaaccgcaaaagctgcgattgcggttcgtgtttttgggaaaagtgacgcgagannnnnnncgttgtggcgcgtaaatttttcttttctcgcaccaaaaggaaaacttttacgcgccgcatttttagggccggtgagtcgccgttttttggctcgtgaaaaggtggcgatccccagccggttcttcttcggctatttcttttcccatcttgtcggatcttttggcttaatcctctcataatcaggtgctcatccttgagtggtttgtctctcgattcgcactttggtctcctccgtttaggtctggttttgggagcttgtttatggtagcctttgcagtgcgattcttgaggtatttggtctttttatacttatctaagagggctttacgtacttcttccttatcttgccgatgagnnnnnnnngtacctttgcaagaaacgtagatgacggtaaaaga aggaggaagaaagtcttcttcctctctcccgatctcatcactctgctcatggtcgtacacttccccgtgcaaataacgatcgggaacctctttcatatcggtggagaggggatagagacgttcacccggtgacaagtgttgcagctcttcgctcatctttgttctcgttctgtattctatatgattctctgcattccaaattcagacagcctgtggactaaccccggataataatccggtgcagcgtgtgaaaatcatacacgaaagtacgaataatacgcccaaccgtctgtctcgaacctcagccgatctccgacaattccggaaccgcaaaagctgcgattgcggttcgtgtttttgggaaaagtgacgcgagatttttttcgttgtggcgcgtaaatttttcttttctcgcaccaaaaggaaaacttttacgcgccgcatttttagggccggtgagtcgccgttttttggctcgtgaaaaggtggcgatccccagccggttcttcttcggctatttcttttcccatcttgtcggatcttttggcttaatcctctcataatcaggtgctcatccttgagtggtttgtctctcgattcgcactttggtctcctccgtttaggtctggttttgggagcttgtttatggtagcctttgcagtgcgattcttgaggtatttggtctttttatacttatctaagagggctttacgtacttcttccttatcttgccgatgagttttttttgtacctttgcaagaaacgtagatgacggtaaaaga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGGAGGAAGAAAGUCUUCUUCCUCUCUCCCGAUCUCAUCACUCUGCUCAUGGUCGUACACUUCCCCGUGCAAAUAACGAUCGGGAACCUCUUUCAUAUCGGUGGAGAGGGGAUAGAGACGUUCACCCGGUGACAAGUGUUGCAGCUCUUCGCUCAUCUUUGUUCUCGUUCUGUAUUCUAUAUGAUUCUCUGCAUUCCAAAUUCAGACAGCCUGUGGACUAACCCCGGAUAAUAAUCCGGUGCAGCGUGUGAAAAUCAUACACGAAAGUACGAAUAAUACGCCCAACCGUCUGUCUCGAACCUCAGCCGAUCUCCGACAAUUCCGGAACCGCAAAAGCUGCGAUUGCGGUUCGUGUUUUUGGGAAAAGUGACGCGAGAUUUUUUUCGUUGUGGCGCGUAAAUUUUUCUUUUCUCGCACCAAAAGGAAAACUUUUACGCGCCGCAUUUUUAGGGCCGGUGAGUCGCCGUUUUUUGGCUCGUGAAAAGGUGGCGAUCCCCAGCCGGUUCUUCUUCGGCUAUUUCUUUUCCCAUCUUGUCGGAUCUUUUGGCUUAAUCCUCUCAUAAUCAGGUGCUCAUCCUUGAGUGGUUUGUCUCUCGAUUCGCACUUUGGUCUCCUCCGUUUAGGUCUGGUUUUGGGAGCUUGUUUAUGGUAGCCUUUGCAGUGCGAUUCUUGAGGUAUUUGGUCUUUUUAUACUUAUCUAAGAGGGCUUUACGUACUUCUUCCUUAUCUUGCCGAUGAGUUUUUUUUGUACCUUUGCAAGAAACGUAGAUGACGGUAAAAGA .((((((((((....)))))))))).((((((((.....(((........))).((((........)))).......))))))))...(((((......((((....(((((((((((.((.(((..(....)..)))..)).((.....))...)))))))))))......(((((..((((((((.((((..((((....(((.....)))))))......((((((....)))))).)))).)......))))))).((....))..)))))...))))..((((((.((((((.(((..((((((..((((((((.....(((((((((.........))))))))).......(((((((((....((((((....))))))(((((((((((((((((((((((........))))))))))..))))))))))))).....(((((((((..(((((((..((((.(....).))))..))))))).....))))))))).............)))))))))..))))))))....)))))).....((((((.((((((((((..(((...(((........)))..)))..))))...((......))........)))))).)))))).........)))....((((((((((((.....(((((((..((((((((((........))))))))))....)))))))...((((((.....))))))......))))))...))))))...)).)))))))))).))))) (-254.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUUUUACCGUCAUCUACGUUUCUUGCAAAGGUACAAAAAAAACUCAUCGGCAAGAUAAGGAAGAAGUACGUAAAGCCCUCUUAGAUAAGUAUAAAAAGACCAAAUACCUCAAGAAUCGCACUGCAAAGGCUACCAUAAACAAGCUCCCAAAACCAGACCUAAACGGAGGAGACCAAAGUGCGAAUCGAGAGACAAACCACUCAAGGAUGAGCACCUGAUUAUGAGAGGAUUAAGCCAAAAGAUCCGACAAGAUGGGAAAAGAAAUAGCCGAAGAAGAACCGGCUGGGGAUCGCCACCUUUUCACGAGCCAAAAAACGGCGACUCACCGGCCCUAAAAAUGCGGCGCGUAAAAGUUUUCCUUUUGGUGCGAGAAAAGAAAAAUUUACGCGCCACAACGAAAAAAAUCUCGCGUCACUUUUCCCAAAAACACGAACCGCAAUCGCAGCUUUUGCGGUUCCGGAAUUGUCGGAGAUCGGCUGAGGUUCGAGACAGACGGUUGGGCGUAUUAUUCGUACUUUCGUGUAUGAUUUUCACACGCUGCACCGGAUUAUUAUCCGGGGUUAGUCCACAGGCUGUCUGAAUUUGGAAUGCAGAGAAUCAUAUAGAAUACAGAACGAGAACAAAGAUGAGCGAAGAGCUGCAACACUUGUCACCGGGUGAACGUCUCUAUCCCCUCUCCACCGAUAUGAAAGAGGUUCCCGAUCGUUAUUUGCACGGGGAAGUGUACGACCAUGAGCAGAGUGAUGAGAUCGGGAGAGAGGAAGAAGACUUUCUUCCUCCU (((((((..(((.........((((((...(((.............))).))))))...((((((.(.((((...((((.....(((((((.........(((((((((((..((.(((((((.....((((..........))))............(((......))).......))))))).))..........((.((((..(((.((...)).))).)))).))....((((......((((((((..(((((((((...................(((((((..(((((...((((........))))...)))))..).))))))..........((((((......(((((.((.(((((((.(((........))).))))))).)).)))))......)))))).))))))))).......(((((((((.........))))))))).....))))))))....)))))))))......(((((((((((((.......((((((......))))))............((.((((((....)))))).))..)))))...)))))))).))))))..(((((....((((........................))))........)))))..)))))))...))))..)))))))).)))..........))).)))))))..((((((((((((((((((.((.((..(....)..)).)).))))).))))..))))))))).(((((((((....))))))))).. (-236.18) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1081 70 21 161 112 NC_002950:PG1831|5end_ATP-dependent_DNA_helicase_RecQ_1925422..1925652_NEGATIVE 738 396 351 67 22 NC_002950:PG1980|5end_hypothetical_protein_2067930..2068160_NEGATIVE 608 420 371 55 6 NC_002950:PG2064|5end_hypothetical_protein_2160550..2160780_POSITIVE 528 420 372 91 43 NC_002950:PG0174|5end_pyridine_nucleotide-disulphide_oxidoreductase_family_protein_197628..197858_NEGATIVE 498 420 371 69 20 NC_002950:PG1799|5end_hypothetical_protein_1894499..1894729_POSITIVE 487 440 391 77 28 NC_002950:PG0313|5end_hypothetical_protein_347366..347596_POSITIVE 454 418 371 87 38 NC_002950:PG1617|5end_hypothetical_protein_1697755..1697985_NEGATIVE 432 330 281 225 170 NC_002950:PG1534|5end_hypothetical_protein_1614147..1614377_NEGATIVE 421 130 86 221 153 NC_002950:PG0728|5end_hypothetical_protein_779807..780037_NEGATIVE 415 385 341 230 188 NC_002950:PG0532|5end_hypothetical_protein_582148..582378_NEGATIVE 404 478 437 216 178 NC_002950:PG0221|5end_hypothetical_protein_255834..256064_NEGATIVE 403 327 281 199 157 NC_002950:PG1285|5end_glucosamine-6-phosphate_deaminase_1362894..1363124_POSITIVE 398 330 286 147 79 NC_002950:PG1398|5end_hypothetical_protein_1480694..1480924_POSITIVE 394 356 312 83 39 NC_002950:PG0213|5end_precorrin-3_methylase/precorrin-8X_methylmutase_250464..250694_NEGATIVE 392 339 292 210 149 NC_002950:PG1251|5end_hypothetical_protein_1327779..1328009_POSITIVE 390 248 201 212 153 NC_002950:PG1952|5end_DedA_family_protein_2042870..2043100_POSITIVE 388 491 452 122 66 NC_002950:PG0326|5end_hypothetical_protein_360974..361204_NEGATIVE 387 457 412 79 24 NC_002950:PG1912|5end_30S_ribosomal_protein_S4_2012380..2012610_NEGATIVE 384 480 434 204 145 NC_002950:PG1481|5end_conjugative_transposon_protein_TraG_1558447..1558677_NEGATIVE 384 359 312 203 150 NC_002950:PG0158|5end_competence_protein_F-related_protein_180175..180405_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 434 500 451 151 96 NC_002950:PG0768|3end_hypothetical_protein_821275..821425_NEGATIVE 423 388 341 105 58 NC_002950:PG1718|3end_hypothetical_protein_1806532..1806682_NEGATIVE 418 529 485 49 9 NC_002950:PG1690|3end_Sua5/YciO/YrdC/YwlC_family_protein_1775171..1775321_NEGATIVE 404 478 437 137 99 NC_002950:PG0222|3end_DNA-binding_protein,_histone-like_family_255913..256063_NEGATIVE 396 528 481 107 30 NC_002950:PG0132|3end_hypothetical_protein_153054..153204_POSITIVE 392 528 481 71 29 NC_002950:PG1630|3end_hypothetical_protein_1712142..1712292_POSITIVE 387 498 451 124 83 NC_002950:PG0766|3end_polyribonucleotide_nucleotidyltransferase_816174..816324_NEGATIVE 385 380 335 89 34 NC_002950:PG1998|3end_polyprenyl_synthetase_2087151..2087301_NEGATIVE 385 459 411 79 20 NC_002950:PG0542|3end_hypothetical_protein_595377..595527_NEGATIVE 382 514 477 43 11 NC_002950:PG0404|3end_hypothetical_protein_439768..439918_NEGATIVE 377 480 435 145 103 NC_002950:PG0099|3end_phenylalanyl-tRNA_synthetase_beta_subunit_115827..115977_NEGATIVE 376 145 102 145 92 NC_002950:PG1754|3end_hypothetical_protein_1842284..1842434_POSITIVE 376 144 103 105 56 NC_002950:PG0032|3end_beta-mannosidase,_putative_37627..37777_NEGATIVE 375 508 462 82 28 NC_002950:PG0425|3end_esterase,_putative_463219..463369_NEGATIVE 374 510 466 149 98 NC_002950:PG0303|3end_ferredoxin_339603..339753_POSITIVE 372 370 322 86 25 NC_002950:PG0230|3end_transaldolase_266114..266264_POSITIVE 370 480 438 95 34 NC_002950:PG0126|3end_type_I_phosphodiesterase/nucleotide_pyrophosphatase_family_protein_146783..146933_NEGATIVE 369 477 435 90 50 NC_002950:PG1942|3end_30S_ribosomal_protein_S12_2027946..2028096_NEGATIVE 369 450 412 94 56 NC_002950:PG0732|3end_hypothetical_protein_783018..783168_POSITIVE 366 509 463 59 12 NC_002950:PG0803|3end_glucosamine-6-phosphate_deaminase_859727..859877_NEGATIVE