Origin IGS: ataagtcggcaatataaatgcccccaaagttgtgtttccaactttgggggcattctttttttgggcagtggtactatcttttccccttttccgtattcggcagaaagatgtaccctgtgtgtactttgtcgc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gcgacaaagtacacacagggtacatctttctgccgaatacggaaaaggggaaaagatagtaccactgcccaaaaaaagaatgcccccaaagttggaaacacaactttgggggcatttatattgccgacttat Mask Tandem Repeat Region ================================================ ataagtcggcaatataaatgcccccaaagttgtgtttccaactttgggggcattctttttttgggcagtggtactatcttttccccttttccgtattcggcagaaagatgtaccctgtgtgtactttgtcgc Find is-nt database================================================ Query_seq: PG1823:PG1824|PG1823:PG1824:hypothetical protein:phosphopyruvate hydratase:-><-:1918111..1918242 132 ataagtcggcaatataaatgcccccaaagttgtgtttccaactttgggggcattctttttttgggcagtggtactatcttttccccttttccgtattcggcagaaagatgtaccctgtgtgtactttgtcgc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1823:PG1824|PG1823:PG1824:hypothetical protein:phosphopyruvate hydratase:-><-:1918111..1918242 132 ataagtcggcaatataaatgcccccaaagttgtgtttccaactttgggggcattctttttttgggcagtggtactatcttttccccttttccgtattcggcagaaagatgtaccctgtgtgtactttgtcgc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1823:PG1824|PG1823:PG1824:hypothetical protein:phosphopyruvate hydratase:-><-:1918111..1918242 132 ataagtcggcaatataaatgcccccaaagttgtgtttccaactttgggggcattctttttttgggcagtggtactatcttttccccttttccgtattcggcagaaagatgtaccctgtgtgtactttgtcgc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ataagtcggcaatataaatgcccccaaagttgtgtttccaactttgggggcattctttttttgggcagtggtactatcttttccccttttccgtattcggcagaaagatgtaccctgtgtgtactttgtcgc Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 14 End: 130 ............. M N A P K V V F P T L G A F F F W A V V L S F P L F R I R Q K D V P C V Y F V .. Protein_Len: 38 Strand: + Start: 18 End: 131 ................. M P P K L C F Q L W G H S F F G Q W Y Y L F P F S V F G R K M Y P V C T L S . Protein_Len: 44 Strand: + Start: 1 End: 132 M S R Q Y K C P Q S C V S N F G G I L F L G S G T I F S P F P Y S A E R C T L C V L C R Protein_Len: 32 Strand: - Start: 1 End: 96 Y T P L I F A G L T T N G V K P A N K K Q A T T S D K G R K R M .................................... ataagtcggcaatataaatgcccccaaagttgtgtttccaactttgggggcattctttttttgggcagtggtactatcttttccccttttccgtattcggcagaaagatgtaccctgtgtgtactttgtcgc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ataagtcggcaatataaatgcccccaaagttgtgtttccaactttgggggcattctttttttgggcagtggtactatcttttccccttttccgtattcggcagaaagatgtaccctgtgtgtactttgtcgc Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -19.8 Tail_Score: -4.19177 Start: 19 End: 52 Full_Region: tgcccaaaaaaagaa tgcccccaaagttg gaaaca caactttgggggca tttatattgccgact ..................tgcccccaaagttggaaacacaactttgggggca................................................................................ Find igs database================================================ Query_seq: PG1823:PG1824|PG1823:PG1824:hypothetical protein:phosphopyruvate hydratase:-><-:1918111..1918242 132 ataagtcggcaatataaatgcccccaaagttgtgtttccaactttgggggcattctttttttgggcagtggtactatcttttccccttttccgtattcggcagaaagatgtaccctgtgtgtactttgtcgc Intra-Species Hit: Count: 1 Min: 1 Max: 132 Len: 132 Subject: NC_002950_PG1823_PG1824|hypothetical protein:phosphopyruvate hydratase|POSITIVE:NEGATIVE|[1918111,1918242]|132 HSP 1 e-value: 9.0E-57 bit: 220.0 Len: 132 Query Start:1 Query End:132 Subject Strand: POSITIVE Subject Start: 1 Subject End: 132 ataagtcggcaatataaatgcccccaaagttgtgtttccaactttgggggcattcnnnnnnngggcagtggtactatcttttccccttttccgtattcggcagaaagatgtaccctgtgtgtactttgtcgc ataagtcggcaatataaatgcccccaaagttgtgtttccaactttgggggcattctttttttgggcagtggtactatcttttccccttttccgtattcggcagaaagatgtaccctgtgtgtactttgtcgc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAGUCGGCAAUAUAAAUGCCCCCAAAGUUGUGUUUCCAACUUUGGGGGCAUUCUUUUUUUGGGCAGUGGUACUAUCUUUUCCCCUUUUCCGUAUUCGGCAGAAAGAUGUACCCUGUGUGUACUUUGUCGC ......((((((....((((((((((((((((......))))))))))))))))........(.((((.(((((.(((((((..((............))..)))))))))))))))).).....)))))). (-52.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCGACAAAGUACACACAGGGUACAUCUUUCUGCCGAAUACGGAAAAGGGGAAAAGAUAGUACCACUGCCCAAAAAAAGAAUGCCCCCAAAGUUGGAAACACAACUUUGGGGGCAUUUAUAUUGCCGACUUAU ((((...........((((((((((((((.(.((............)).).)))))).))))).)))..........(((((((((((((((((......)))))))))))))))))...))))........ (-50.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 393 109 63 81 26 NC_002950:PG0484|5end_hypothetical_protein_524858..525088_NEGATIVE 367 108 61 79 30 NC_002950:PG1356|5end_hypothetical_protein_1435849..1436079_NEGATIVE 349 69 23 122 71 NC_002950:PG0554|5end_hypothetical_protein_611465..611695_POSITIVE 335 130 81 229 166 NC_002950:PG1348|5end_conserved_hypothetical_protein_TIGR00147_1428608..1428838_POSITIVE 332 59 20 229 170 NC_002950:PG1983|5end_CRISPR-associated_TM1791_family_protein_2073442..2073672_NEGATIVE 327 100 54 213 170 NC_002950:PG0923|5end_ribosome-binding_factor_A_982814..983044_NEGATIVE 320 83 41 47 2 NC_002950:PG2182|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_A_2291155..2291385_NEGATIVE 317 84 45 89 43 NC_002950:PG1253|5end_DNA_ligase,_NAD-dependent_1331606..1331836_NEGATIVE 316 70 23 169 123 NC_002950:PG1747|5end_ribose_5-phosphate_isomerase_B,_putative_1832815..1833045_NEGATIVE 315 84 44 49 9 NC_002950:PG0097|5end_hypothetical_protein_115748..115978_NEGATIVE 314 90 41 77 9 NC_002950:PG1347|5end_hypothetical_protein_1428116..1428346_POSITIVE 313 101 61 212 169 NC_002950:PG0264|5end_glycosyl_transferase,_group_2_family_protein_296975..297205_POSITIVE 312 68 24 221 175 NC_002950:PG0229|5end_hypothetical_protein_264872..265102_POSITIVE 310 128 85 210 168 NC_002950:PG1814|5end_DNA_primase_1910627..1910857_NEGATIVE 308 99 51 65 7 NC_002950:PG1017|5end_pyruvate_phosphate_dikinase_1076138..1076368_POSITIVE 307 118 71 70 13 NC_002950:PG1288|5end_GDP-mannose_4,6-dehydratase_1365963..1366193_POSITIVE 306 80 33 178 127 NC_002950:PG1488|5end_hypothetical_protein_1561747..1561977_POSITIVE 306 102 63 192 155 NC_002950:PG1479|5end_conjugative_transposon_protein_TraJ_1555234..1555464_NEGATIVE 305 104 61 92 53 NC_002950:PG1027|5end_hypothetical_protein_1089439..1089669_NEGATIVE 303 102 61 163 108 NC_002950:PG0633|5end_hypothetical_protein_685301..685531_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 522 54 17 74 37 NC_002950:PG1823|3end_hypothetical_protein_1918050..1918200_POSITIVE 341 72 31 37 1 NC_002950:PG0098|3end_hypothetical_protein_115745..115895_NEGATIVE 325 49 8 151 112 NC_002950:PG1570|3end_rhodanese-like_domain_protein_1648582..1648732_NEGATIVE 317 84 45 101 55 NC_002950:PG1254|3end_acetyltransferase,_GNAT_family_1331594..1331744_NEGATIVE 316 70 23 82 36 NC_002950:PG1748|3end_transketolase_1832902..1833052_NEGATIVE 311 103 64 85 42 NC_002950:PG1226|3end_peptidyl-prolyl_cis-trans_isomerase,_cyclophilin-type_1304048..1304198_POSITIVE 308 108 62 76 31 NC_002950:PG0605|3end_hypothetical_protein_661165..661315_NEGATIVE 306 80 33 150 99 NC_002950:PG1487|3end_hypothetical_protein_1561799..1561949_POSITIVE 299 80 32 108 67 NC_002950:PG1216|3end_hypothetical_protein_1295665..1295815_POSITIVE 298 110 61 69 6 NC_002950:PG0906|3end_lipoprotein,_putative_971148..971298_POSITIVE 298 110 63 130 78 NC_002950:PG0097|3end_hypothetical_protein_115056..115206_NEGATIVE 297 108 62 65 6 NC_002950:PG1253|3end_DNA_ligase,_NAD-dependent_1329597..1329747_NEGATIVE 296 73 32 77 33 NC_002950:PG0634|3end_ThiJ/PfpI_family_protein_686768..686918_POSITIVE 292 102 62 145 96 NC_002950:PG0975|3end_PhoH_family_protein_1038356..1038506_POSITIVE 292 50 4 121 60 NC_002950:PG0157|3end_regulatory_protein_RecX_180271..180421_POSITIVE 291 130 82 70 5 NC_002950:PG0072|3end_UDP-3-O-[3-hydroxymyristoyl]_glucosamine_N-acyltransferase_86098..86248_NEGATIVE 286 119 71 121 76 NC_002950:PG1840|3end_hypothetical_protein_1937295..1937445_POSITIVE 286 109 64 98 57 NC_002950:PG1675|3end_hypothetical_protein_1760360..1760510_POSITIVE 283 55 20 112 67 NC_002950:PG0544|3end_type_I_restriction-modification_system,_M_subunit,_putative_598078..598228_POSITIVE 281 130 81 145 94 NC_002950:PG0377|3end_30S_ribosomal_protein_S2_407845..407995_POSITIVE