Origin IGS: aatctattctcaatctacaatgtggtcatactatacaaatgtaaaaaaaaggacggtgtatcatcatgcgcaaattactgtctcgtttgcaatatttggctcagccacgtactttgtattcagccacggtcttagtgccttgtactttacgcatccttacgcacctaaaaggggccatgccaaataatttgacacggccccctcgttattgtatatgtgttgatcgaattagattgtctctttcaga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tctgaaagagacaatctaattcgatcaacacatatacaataacgagggggccgtgtcaaattatttggcatggccccttttaggtgcgtaaggatgcgtaaagtacaaggcactaagaccgtggctgaatacaaagtacgtggctgagccaaatattgcaaacgagacagtaatttgcgcatgatgatacaccgtccttttttttacatttgtatagtatgaccacattgtagattgagaatagatt Mask Tandem Repeat Region ================================================ aatctattctcaatctacaatgtggtcatactatacaaatgtaaaaaaaaggacggtgtatcatcatgcgcaaattactgtctcgtttgcaatatttggctcagccacgtactttgtattcagccacggtcttagtgccttgtactttacgcatccttacgcacctaaaaggggccatgccaaataatttgacacggccccctcgttattgtatatgtgttgatcgaattagattgtctctttcaga Find is-nt database================================================ Query_seq: PG1808:PG1809|PG1808:PG1809:guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase:2-oxoglutarate oxidoreductase, gamma subunit:<-<-:1905334..1905580 247 aatctattctcaatctacaatgtggtcatactatacaaatgtaaaaaaaaggacggtgtatcatcatgcgcaaattactgtctcgtttgcaatatttggctcagccacgtactttgtattcagccacggtcttagtgccttgtactttacgcatccttacgcacctaaaaggggccatgccaaataatttgacacggccccctcgttattgtatatgtgttgatcgaattagattgtctctttcaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1808:PG1809|PG1808:PG1809:guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase:2-oxoglutarate oxidoreductase, gamma subunit:<-<-:1905334..1905580 247 aatctattctcaatctacaatgtggtcatactatacaaatgtaaaaaaaaggacggtgtatcatcatgcgcaaattactgtctcgtttgcaatatttggctcagccacgtactttgtattcagccacggtcttagtgccttgtactttacgcatccttacgcacctaaaaggggccatgccaaataatttgacacggccccctcgttattgtatatgtgttgatcgaattagattgtctctttcaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1808:PG1809|PG1808:PG1809:guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase:2-oxoglutarate oxidoreductase, gamma subunit:<-<-:1905334..1905580 247 aatctattctcaatctacaatgtggtcatactatacaaatgtaaaaaaaaggacggtgtatcatcatgcgcaaattactgtctcgtttgcaatatttggctcagccacgtactttgtattcagccacggtcttagtgccttgtactttacgcatccttacgcacctaaaaggggccatgccaaataatttgacacggccccctcgttattgtatatgtgttgatcgaattagattgtctctttcaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatctattctcaatctacaatgtggtcatactatacaaatgtaaaaaaaaggacggtgtatcatcatgcgcaaattactgtctcgtttgcaatatttggctcagccacgtactttgtattcagccacggtcttagtgccttgtactttacgcatccttacgcacctaaaaggggccatgccaaataatttgacacggccccctcgttattgtatatgtgttgatcgaattagattgtctctttcaga Predict ORF larger than 30AA ================================================ Protein_Len: 40 Strand: + Start: 13 End: 132 ............ M Y N V V I L Y K C K K K D G V S S C A N Y C L V C N I W L S H V L C I Q P R S ................................................................................................................... Protein_Len: 60 Strand: + Start: 2 End: 184 . M Y S Q S T M W S Y Y T N V K K R T V Y H H A Q I T V S F A I F G S A T Y F V F S H G L S A L Y F T H P Y A P K R G H A ............................................................... Protein_Len: 60 Strand: + Start: 60 End: 245 ........................................................... M I M R K L L S R L Q Y L A Q P R T L Y S A T V L V P C T L R I L T H L K G A M P N N L T R P P R Y C I C V D R I R L S .. aatctattctcaatctacaatgtggtcatactatacaaatgtaaaaaaaaggacggtgtatcatcatgcgcaaattactgtctcgtttgcaatatttggctcagccacgtactttgtattcagccacggtcttagtgccttgtactttacgcatccttacgcacctaaaaggggccatgccaaataatttgacacggccccctcgttattgtatatgtgttgatcgaattagattgtctctttcaga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatctattctcaatctacaatgtggtcatactatacaaatgtaaaaaaaaggacggtgtatcatcatgcgcaaattactgtctcgtttgcaatatttggctcagccacgtactttgtattcagccacggtcttagtgccttgtactttacgcatccttacgcacctaaaaggggccatgccaaataatttgacacggccccctcgttattgtatatgtgttgatcgaattagattgtctctttcaga Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 75 HP_score: -10.5 Tail_Score: -3.02314 Start: 170 End: 202 Full_Region: CCTTACGCACCTAAA AGGGGCCATGCCAAA TAA TTTGACACGGCCCCC TCGTTATTGTATATG .........................................................................................................................................................................AGGGGCCATGCCAAATAATTTGACACGGCCCCC............................................. TransTerm Strand: - Conf: 100 HP_score: -18.4 Tail_Score: -4.19817 Start: 171 End: 201 Full_Region: atatacaataacgag ggggccgtgtcaaa tta tttggcatggcccc ttttaggtgcgtaag ..........................................................................................................................................................................ggggccgtgtcaaattatttggcatggcccc.............................................. Find igs database================================================ Query_seq: PG1808:PG1809|PG1808:PG1809:guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase:2-oxoglutarate oxidoreductase, gamma subunit:<-<-:1905334..1905580 247 aatctattctcaatctacaatgtggtcatactatacaaatgtaaaaaaaaggacggtgtatcatcatgcgcaaattactgtctcgtttgcaatatttggctcagccacgtactttgtattcagccacggtcttagtgccttgtactttacgcatccttacgcacctaaaaggggccatgccaaataatttgacacggccccctcgttattgtatatgtgttgatcgaattagattgtctctttcaga Intra-Species Hit: Count: 1 Min: 1 Max: 247 Len: 247 Subject: NC_002950_PG1808_PG1809|guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase:2-oxoglutarate oxidoreductase, gamma subunit|NEGATIVE:NEGATIVE|[1905334,1905580]|247 HSP 1 e-value: 1.0E-123 bit: 442.0 Len: 247 Query Start:1 Query End:247 Subject Strand: POSITIVE Subject Start: 1 Subject End: 247 aatctattctcaatctacaatgtggtcatactatacaaatgtnnnnnnnnggacggtgtatcatcatgcgcaaattactgtctcgtttgcaatatttggctcagccacgtactttgtattcagccacggtcttagtgccttgtactttacgcatccttacgcacctaaaaggggccatgccaaataatttgacacggccccctcgttattgtatatgtgttgatcgaattagattgtctctttcaga aatctattctcaatctacaatgtggtcatactatacaaatgtaaaaaaaaggacggtgtatcatcatgcgcaaattactgtctcgtttgcaatatttggctcagccacgtactttgtattcagccacggtcttagtgccttgtactttacgcatccttacgcacctaaaaggggccatgccaaataatttgacacggccccctcgttattgtatatgtgttgatcgaattagattgtctctttcaga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUCUAUUCUCAAUCUACAAUGUGGUCAUACUAUACAAAUGUAAAAAAAAGGACGGUGUAUCAUCAUGCGCAAAUUACUGUCUCGUUUGCAAUAUUUGGCUCAGCCACGUACUUUGUAUUCAGCCACGGUCUUAGUGCCUUGUACUUUACGCAUCCUUACGCACCUAAAAGGGGCCAUGCCAAAUAAUUUGACACGGCCCCCUCGUUAUUGUAUAUGUGUUGAUCGAAUUAGAUUGUCUCUUUCAGA ..........(((((((.....((((((((((((((((..........(((((..(((((....((.((((.....(((((((.(..((((((((.((((...)))).)))..)))))..)))..)))))....))))..)).....))))).)))))............((((((.((.(((.....))).)).)))))).......))))))).))).))))))...)))))))........... (-63.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUGAAAGAGACAAUCUAAUUCGAUCAACACAUAUACAAUAACGAGGGGGCCGUGUCAAAUUAUUUGGCAUGGCCCCUUUUAGGUGCGUAAGGAUGCGUAAAGUACAAGGCACUAAGACCGUGGCUGAAUACAAAGUACGUGGCUGAGCCAAAUAUUGCAAACGAGACAGUAAUUUGCGCAUGAUGAUACACCGUCCUUUUUUUUACAUUUGUAUAGUAUGACCACAUUGUAGAUUGAGAAUAGAUU ............((((((.(((((((.(((..(((((((....((((((((((((((((.....))))))))))))))))..((((..(.(..(((((((((.(((.((.(((.......))).))........((((..((((...))))..))))............))).)))))))))..).)..))))................)))))))..(((....)))))).)))))))..)))))) (-72.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 955 140 91 231 182 NC_002950:PG1808|5end_guanosine-3',5'-bis(diphosphate)_3'-pyrophosphohydrolase_1905243..1905473_NEGATIVE 367 209 161 181 130 NC_002950:PG0283|5end_efflux_transporter,_MFP_component,_RND_family_318245..318475_NEGATIVE 365 100 52 181 133 NC_002950:PG1408|5end_heavy_metal_efflux_pump,_CzcD_family_1491861..1492091_POSITIVE 348 205 162 63 7 NC_002950:PG0160|5end_hypothetical_protein_184085..184315_NEGATIVE 342 218 171 190 148 NC_002950:PG0480|5end_precorrin-2_C20-methyltransferase,_putative_521319..521549_NEGATIVE 333 210 164 207 150 NC_002950:PG0394|5end_DNA-directed_RNA_polymerase_beta_subunit_425049..425279_POSITIVE 330 200 152 136 97 NC_002950:PG0767|5end_4-alpha-glucanotransferase_820533..820763_NEGATIVE 325 167 121 222 172 NC_002950:PG0058|5end_nicotinic_acid_mononucleotide_adenyltransferase_70919..71149_POSITIVE 321 100 54 66 28 NC_002950:PG0159|5end_endopeptidase_PepO_183164..183394_NEGATIVE 320 180 131 63 2 NC_002950:PG1923|5end_50S_ribosomal_protein_L6_2017941..2018171_NEGATIVE 319 89 50 200 168 NC_002950:PG0219|5end_hypothetical_protein_255558..255788_NEGATIVE 317 140 94 104 45 NC_002950:PG2109|5end_thiamine-phosphate_pyrophosphorylase_2219452..2219682_NEGATIVE 317 180 134 58 3 NC_002950:PG0491|5end_hypothetical_protein_532184..532414_POSITIVE 316 157 118 176 121 NC_002950:PG1849|5end_DNA_repair_protein_RecN_1949034..1949264_NEGATIVE 316 198 155 220 178 NC_002950:PG0679|5end_outer_membrane_efflux_protein_729594..729824_POSITIVE 313 184 145 228 181 NC_002950:PG0625|5end_GTP_cyclohydrolase_I_678588..678818_POSITIVE 313 232 198 158 122 NC_002950:PG0156|5end_modification_methylase,_HemK_family_178828..179058_POSITIVE 311 198 151 143 99 NC_002950:PG0229|5end_hypothetical_protein_264872..265102_POSITIVE 310 129 82 228 180 NC_002950:PG2032|5end_primosomal_protein_N'_2133381..2133611_NEGATIVE 309 128 85 229 189 NC_002950:PG0090|5end_Dps_family_protein_105524..105754_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 412 205 165 46 1 NC_002950:PG1950|3end_hypothetical_protein_2040840..2040990_POSITIVE 405 227 188 47 10 NC_002950:PG1235|3end_epimerase/reductase,_putative_1310300..1310450_NEGATIVE 382 180 135 45 9 NC_002950:PG0604|3end_4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase_661211..661361_POSITIVE 349 214 171 80 34 NC_002950:PG1729|3end_thiol_peroxidase_1818342..1818492_NEGATIVE 342 218 171 77 35 NC_002950:PG0481|3end_2-amino-3-ketobutyrate_coenzyme_A_ligase_521432..521582_NEGATIVE 337 201 165 73 31 NC_002950:PG0291|3end_hypothetical_protein_326622..326772_POSITIVE 333 210 164 95 38 NC_002950:PG0393|3end_ribosomal_protein_L7/L12_425017..425167_POSITIVE 332 145 102 56 14 NC_002950:PG0317|3end_peptidase,_M49_family_349771..349921_NEGATIVE 323 228 188 76 42 NC_002950:PG2029|3end_hypothetical_protein_2129202..2129352_POSITIVE 321 100 54 47 9 NC_002950:PG0160|3end_hypothetical_protein_183183..183333_NEGATIVE 319 89 50 145 113 NC_002950:PG0221|3end_hypothetical_protein_255613..255763_NEGATIVE 317 140 94 107 48 NC_002950:PG2110|3end_thiamine_biosynthesis_protein_ThiC_2219449..2219599_NEGATIVE 312 109 62 109 54 NC_002950:PG0401|3end_hypothetical_protein_437765..437915_NEGATIVE 311 240 195 63 20 NC_002950:PG0768|3end_hypothetical_protein_821275..821425_NEGATIVE 310 201 161 61 17 NC_002950:PG0034|3end_thioredoxin_42235..42385_NEGATIVE 297 240 191 102 49 NC_002950:PG1797|3end_DNA-binding_response_regulator/sensor_histidine_kinase_1892978..1893128_POSITIVE 297 100 51 64 15 NC_002950:PG1392|3end_rod_shape-determining_protein_RodA,_putative_1472959..1473109_NEGATIVE 297 150 102 94 44 NC_002950:PG0544|3end_type_I_restriction-modification_system,_M_subunit,_putative_598078..598228_POSITIVE 296 209 170 45 6 NC_002950:PG1297|3end_ribosomal_protein_S1_1377710..1377860_POSITIVE 296 201 165 38 8 NC_002950:PG1003|3end_hypothetical_protein_1060304..1060454_POSITIVE