Origin IGS: cgtttcgaagttatttatcgtttctgtcagccaaaagtacaaaaagcccttccttaatcaatagagagtagtcgattccgtcgatcggccggccaagagtcgagagggaaaagtgctcagaacaagtaaaaaaatgaccgtcatagctatgcatgaatgaagaacggcaacctgtgttctctttcgttttttgatgagtataaggtaaaagcaaaatctttcgatatgggtcattttcgaagctgaaaaaaacggaatattcaccgaaaaatatgcgccaaaaaagttttcaacaacgaagaagtgtttggaatgattccaaataagcacttctgagagatcgaaattcgaggtaaagtacgtaacatgacagcctttttcagatcgaaaagctctttttattagttcgatgaacaatctatatattaatcatttttgatttatatataaatcgttttcattttgtatataaatgattttcaataaatatatagatcgaaaatgatttgtatataaatcacaacttcctaaaggctttttcttgaggggataaaacagaggcacccatcggattttctcttgaatccgacgggtgccttatgtatcaggtgagctatcgaaagactatacggctttactcctcatagaataaaatccgctttgtccgcatgttgcgaccatatatgctgatacgggcaggacacaatagacctgcggccaaacgtgtaatagagtaacggagtacatatctgttttcattgagtcgttagtaaagtattttcgttgataaattagctttctccaaatatatggacatacattcatgagggttttctgccactctgcaaaagtagcaaaaaagctccttcccgacgagtccgattcggacactcgatcaggggtacaaacaaaaatacgaaccaaaaggctttcgctcttagttcgtattcttgagaattgaggtcggttatatagcccgattacgtcgtttcctttgctgaaaatatgccgagatatgactcagccggcgtcttcaaatgttacccaagtctcggtttgttcctgctattacggactgattcggca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgccgaatcagtccgtaatagcaggaacaaaccgagacttgggtaacatttgaagacgccggctgagtcatatctcggcatattttcagcaaaggaaacgacgtaatcgggctatataaccgacctcaattctcaagaatacgaactaagagcgaaagccttttggttcgtatttttgtttgtacccctgatcgagtgtccgaatcggactcgtcgggaaggagcttttttgctacttttgcagagtggcagaaaaccctcatgaatgtatgtccatatatttggagaaagctaatttatcaacgaaaatactttactaacgactcaatgaaaacagatatgtactccgttactctattacacgtttggccgcaggtctattgtgtcctgcccgtatcagcatatatggtcgcaacatgcggacaaagcggattttattctatgaggagtaaagccgtatagtctttcgatagctcacctgatacataaggcacccgtcggattcaagagaaaatccgatgggtgcctctgttttatcccctcaagaaaaagcctttaggaagttgtgatttatatacaaatcattttcgatctatatatttattgaaaatcatttatatacaaaatgaaaacgatttatatataaatcaaaaatgattaatatatagattgttcatcgaactaataaaaagagcttttcgatctgaaaaaggctgtcatgttacgtactttacctcgaatttcgatctctcagaagtgcttatttggaatcattccaaacacttcttcgttgttgaaaacttttttggcgcatatttttcggtgaatattccgtttttttcagcttcgaaaatgacccatatcgaaagattttgcttttaccttatactcatcaaaaaacgaaagagaacacaggttgccgttcttcattcatgcatagctatgacggtcatttttttacttgttctgagcacttttccctctcgactcttggccggccgatcgacggaatcgactactctctattgattaaggaagggctttttgtacttttggctgacagaaacgataaataacttcgaaacg Mask Tandem Repeat Region ================================================ cgtttcgaagttatttatcgtttctgtcagccaaaagtacaaaaagcccttccttaatcaatagagagtagtcgattccgtcgatcggccggccaagagtcgagagggaaaagtgctcagaacaagtaaaaaaatgaccgtcatagctatgcatgaatgaagaacggcaacctgtgttctctttcgttttttgatgagtataaggtaaaagcaaaatctttcgatatgggtcattttcgaagctgaaaaaaacggaatattcaccgaaaaatatgcgccaaaaaagttttcaacaacgaagaagtgtttggaatgattccaaataagcacttctgagagatcgaaattcgaggtaaagtacgtaacatgacagcctttttcagatcgaaaagctctttttattagttcgatgaacaatctatatattaatcatttttgatttatatataaatcgttttcattttgtatataaatgattttcaataaatatatagatcgaaaatgatttgtatataaatcacaacttcctaaaggctttttcttgaggggataaaacagaggcacccatcggattttctcttgaatccgacgggtgccttatgtatcaggtgagctatcgaaagactatacggctttactcctcatagaataaaatccgctttgtccgcatgttgcgaccatatatgctgatacgggcaggacacaatagacctgcggccaaacgtgtaatagagtaacggagtacatatctgttttcattgagtcgttagtaaagtattttcgttgataaattagctttctccaaatatatggacatacattcatgagggttttctgccactctgcaaaagtagcaaaaaagctccttcccgacgagtccgattcggacactcgatcaggggtacaaacaaaaatacgaaccaaaaggctttcgctcttagttcgtattcttgagaattgaggtcggttatatagcccgattacgtcgtttcctttgctgaaaatatgccgagatatgactcagccggcgtcttcaaatgttacccaagtctcggtttgttcctgctattacggactgattcggca Find is-nt database================================================ Query_seq: PG1743:PG1745|PG1743:PG1745:2-dehydro-3-deoxyphosphooctonate aldolase:phosphoribulokinase family protein:<-<-:1828439..1829524 1086 cgtttcgaagttatttatcgtttctgtcagccaaaagtacaaaaagcccttccttaatcaatagagagtagtcgattccgtcgatcggccggccaagagtcgagagggaaaagtgctcagaacaagtaaaaaaatgaccgtcatagctatgcatgaatgaagaacggcaacctgtgttctctttcgttttttgatgagtataaggtaaaagcaaaatctttcgatatgggtcattttcgaagctgaaaaaaacggaatattcaccgaaaaatatgcgccaaaaaagttttcaacaacgaagaagtgtttggaatgattccaaataagcacttctgagagatcgaaattcgaggtaaagtacgtaacatgacagcctttttcagatcgaaaagctctttttattagttcgatgaacaatctatatattaatcatttttgatttatatataaatcgttttcattttgtatataaatgattttcaataaatatatagatcgaaaatgatttgtatataaatcacaacttcctaaaggctttttcttgaggggataaaacagaggcacccatcggattttctcttgaatccgacgggtgccttatgtatcaggtgagctatcgaaagactatacggctttactcctcatagaataaaatccgctttgtccgcatgttgcgaccatatatgctgatacgggcaggacacaatagacctgcggccaaacgtgtaatagagtaacggagtacatatctgttttcattgagtcgttagtaaagtattttcgttgataaattagctttctccaaatatatggacatacattcatgagggttttctgccactctgcaaaagtagcaaaaaagctccttcccgacgagtccgattcggacactcgatcaggggtacaaacaaaaatacgaaccaaaaggctttcgctcttagttcgtattcttgagaattgaggtcggttatatagcccgattacgtcgtttcctttgctgaaaatatgccgagatatgactcagccggcgtcttcaaatgttacccaagtctcggtttgttcctgctattacggactgattcggca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1743:PG1745|PG1743:PG1745:2-dehydro-3-deoxyphosphooctonate aldolase:phosphoribulokinase family protein:<-<-:1828439..1829524 1086 cgtttcgaagttatttatcgtttctgtcagccaaaagtacaaaaagcccttccttaatcaatagagagtagtcgattccgtcgatcggccggccaagagtcgagagggaaaagtgctcagaacaagtaaaaaaatgaccgtcatagctatgcatgaatgaagaacggcaacctgtgttctctttcgttttttgatgagtataaggtaaaagcaaaatctttcgatatgggtcattttcgaagctgaaaaaaacggaatattcaccgaaaaatatgcgccaaaaaagttttcaacaacgaagaagtgtttggaatgattccaaataagcacttctgagagatcgaaattcgaggtaaagtacgtaacatgacagcctttttcagatcgaaaagctctttttattagttcgatgaacaatctatatattaatcatttttgatttatatataaatcgttttcattttgtatataaatgattttcaataaatatatagatcgaaaatgatttgtatataaatcacaacttcctaaaggctttttcttgaggggataaaacagaggcacccatcggattttctcttgaatccgacgggtgccttatgtatcaggtgagctatcgaaagactatacggctttactcctcatagaataaaatccgctttgtccgcatgttgcgaccatatatgctgatacgggcaggacacaatagacctgcggccaaacgtgtaatagagtaacggagtacatatctgttttcattgagtcgttagtaaagtattttcgttgataaattagctttctccaaatatatggacatacattcatgagggttttctgccactctgcaaaagtagcaaaaaagctccttcccgacgagtccgattcggacactcgatcaggggtacaaacaaaaatacgaaccaaaaggctttcgctcttagttcgtattcttgagaattgaggtcggttatatagcccgattacgtcgtttcctttgctgaaaatatgccgagatatgactcagccggcgtcttcaaatgttacccaagtctcggtttgttcctgctattacggactgattcggca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1743:PG1745|PG1743:PG1745:2-dehydro-3-deoxyphosphooctonate aldolase:phosphoribulokinase family protein:<-<-:1828439..1829524 1086 cgtttcgaagttatttatcgtttctgtcagccaaaagtacaaaaagcccttccttaatcaatagagagtagtcgattccgtcgatcggccggccaagagtcgagagggaaaagtgctcagaacaagtaaaaaaatgaccgtcatagctatgcatgaatgaagaacggcaacctgtgttctctttcgttttttgatgagtataaggtaaaagcaaaatctttcgatatgggtcattttcgaagctgaaaaaaacggaatattcaccgaaaaatatgcgccaaaaaagttttcaacaacgaagaagtgtttggaatgattccaaataagcacttctgagagatcgaaattcgaggtaaagtacgtaacatgacagcctttttcagatcgaaaagctctttttattagttcgatgaacaatctatatattaatcatttttgatttatatataaatcgttttcattttgtatataaatgattttcaataaatatatagatcgaaaatgatttgtatataaatcacaacttcctaaaggctttttcttgaggggataaaacagaggcacccatcggattttctcttgaatccgacgggtgccttatgtatcaggtgagctatcgaaagactatacggctttactcctcatagaataaaatccgctttgtccgcatgttgcgaccatatatgctgatacgggcaggacacaatagacctgcggccaaacgtgtaatagagtaacggagtacatatctgttttcattgagtcgttagtaaagtattttcgttgataaattagctttctccaaatatatggacatacattcatgagggttttctgccactctgcaaaagtagcaaaaaagctccttcccgacgagtccgattcggacactcgatcaggggtacaaacaaaaatacgaaccaaaaggctttcgctcttagttcgtattcttgagaattgaggtcggttatatagcccgattacgtcgtttcctttgctgaaaatatgccgagatatgactcagccggcgtcttcaaatgttacccaagtctcggtttgttcctgctattacggactgattcggca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cgtttcgaagttatttatcgtttctgtcagccaaaagtacaaaaagcccttccttaatcaatagagagtagtcgattccgtcgatcggccggccaagagtcgagagggaaaagtgctcagaacaagtaaaaaaatgaccgtcatagctatgcatgaatgaagaacggcaacctgtgttctctttcgttttttgatgagtataaggtaaaagcaaaatctttcgatatgggtcattttcgaagctgaaaaaaacggaatattcaccgaaaaatatgcgccaaaaaagttttcaacaacgaagaagtgtttggaatgattccaaataagcacttctgagagatcgaaattcgaggtaaagtacgtaacatgacagcctttttcagatcgaaaagctctttttattagttcgatgaacaatctatatattaatcatttttgatttatatataaatcgttttcattttgtatataaatgattttcaataaatatatagatcgaaaatgatttgtatataaatcacaacttcctaaaggctttttcttgaggggataaaacagaggcacccatcggattttctcttgaatccgacgggtgccttatgtatcaggtgagctatcgaaagactatacggctttactcctcatagaataaaatccgctttgtccgcatgttgcgaccatatatgctgatacgggcaggacacaatagacctgcggccaaacgtgtaatagagtaacggagtacatatctgttttcattgagtcgttagtaaagtattttcgttgataaattagctttctccaaatatatggacatacattcatgagggttttctgccactctgcaaaagtagcaaaaaagctccttcccgacgagtccgattcggacactcgatcaggggtacaaacaaaaatacgaaccaaaaggctttcgctcttagttcgtattcttgagaattgaggtcggttatatagcccgattacgtcgtttcctttgctgaaaatatgccgagatatgactcagccggcgtcttcaaatgttacccaagtctcggtttgttcctgctattacggactgattcggca Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 224 End: 313 ............................................................................................................................................................................................................................... M W V I F E A E K N G I F T E K Y A P K K F S T T K K C L E ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 36 Strand: + Start: 216 End: 323 ....................................................................................................................................................................................................................... M F R Y G S F S K L K K T E Y S P K N M R Q K S F Q Q R R S V W N D S K ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 48 Strand: + Start: 808 End: 951 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M W T Y I H E G F L P L C K S S K K A P S R R V R F G H S I R G T N K N T N Q K A F A L S S Y S ....................................................................................................................................... Protein_Len: 56 Strand: + Start: 791 End: 958 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M S F L Q I Y G H T F M R V F C H S A K V A K K L L P D E S D S D T R S G V Q T K I R T K R L S L L V R I L E N ................................................................................................................................ Protein_Len: 35 Strand: + Start: 980 End: 1084 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M T S F P L L K I C R D M T Q P A S S N V T Q V S V C S C Y Y G L I R .. Protein_Len: 49 Strand: - Start: 825 End: 971 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ S P K R G S Q L L L L F A G E R R T R N P C E I L P V F L F V F W F A K A R L E Y E Q S N L D T M ................................................................................................................... Protein_Len: 38 Strand: - Start: 770 End: 883 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Y L I K T S L N A K E L Y I S M C E H P N E A V R C F Y C F L E K G V L G M ........................................................................................................................................................................................................... Protein_Len: 35 Strand: - Start: 645 End: 749 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... L I F D A K D A H Q S W I H Q Y P C S V I S R R G F T Y Y L L P T C M ................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 31 Strand: - Start: 638 End: 730 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. E E Y F L I R K T R M N R G Y I S I R A P C L L G A A L R T M .................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 628 End: 1017 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... W E A F T A F F S R G S S D S E S V R D P T C V F I R V L L S E S K T R I R S F Q P R N Y L G I V D N G K S F I H R S M ..................................................................... Protein_Len: 51 Strand: - Start: 433 End: 585 ................................................................................................................................................................................................................................................................................................................................................................................................................................................ K Q N I Y L D N E N Q I Y I I K L L Y I S R F H N T Y L D C S G L P K K K L P Y F L P V W R I K E Q M ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 40 Strand: - Start: 264 End: 383 ....................................................................................................................................................................................................................................................................... R F I H A L F T K L L S S T N P I I G F L C K Q S I S I R P L T R L M V A K K M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 61 End: 426 ............................................................ F F P I N V S F Y A G F F N E V V F F H K S H N W I L V E S L D F N S T F Y T V H C G K E S R F A R K I L E I F L R Y M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 14 End: 274 ............. L T S L S F H E S C T F F H G D Y S H M F S S R C G T N E K T K Q H T Y P L L L I K R Y P D N E F S F F V S Y E G F F M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ cgtttcgaagttatttatcgtttctgtcagccaaaagtacaaaaagcccttccttaatcaatagagagtagtcgattccgtcgatcggccggccaagagtcgagagggaaaagtgctcagaacaagtaaaaaaatgaccgtcatagctatgcatgaatgaagaacggcaacctgtgttctctttcgttttttgatgagtataaggtaaaagcaaaatctttcgatatgggtcattttcgaagctgaaaaaaacggaatattcaccgaaaaatatgcgccaaaaaagttttcaacaacgaagaagtgtttggaatgattccaaataagcacttctgagagatcgaaattcgaggtaaagtacgtaacatgacagcctttttcagatcgaaaagctctttttattagttcgatgaacaatctatatattaatcatttttgatttatatataaatcgttttcattttgtatataaatgattttcaataaatatatagatcgaaaatgatttgtatataaatcacaacttcctaaaggctttttcttgaggggataaaacagaggcacccatcggattttctcttgaatccgacgggtgccttatgtatcaggtgagctatcgaaagactatacggctttactcctcatagaataaaatccgctttgtccgcatgttgcgaccatatatgctgatacgggcaggacacaatagacctgcggccaaacgtgtaatagagtaacggagtacatatctgttttcattgagtcgttagtaaagtattttcgttgataaattagctttctccaaatatatggacatacattcatgagggttttctgccactctgcaaaagtagcaaaaaagctccttcccgacgagtccgattcggacactcgatcaggggtacaaacaaaaatacgaaccaaaaggctttcgctcttagttcgtattcttgagaattgaggtcggttatatagcccgattacgtcgtttcctttgctgaaaatatgccgagatatgactcagccggcgtcttcaaatgttacccaagtctcggtttgttcctgctattacggactgattcggca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 423 End: 452 ......................................................................................................................................................................................................................................................................................................................................................................................................................................ATTTATATATAAATCAAAAATGATTAATAT.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cgtttcgaagttatttatcgtttctgtcagccaaaagtacaaaaagcccttccttaatcaatagagagtagtcgattccgtcgatcggccggccaagagtcgagagggaaaagtgctcagaacaagtaaaaaaatgaccgtcatagctatgcatgaatgaagaacggcaacctgtgttctctttcgttttttgatgagtataaggtaaaagcaaaatctttcgatatgggtcattttcgaagctgaaaaaaacggaatattcaccgaaaaatatgcgccaaaaaagttttcaacaacgaagaagtgtttggaatgattccaaataagcacttctgagagatcgaaattcgaggtaaagtacgtaacatgacagcctttttcagatcgaaaagctctttttattagttcgatgaacaatctatatattaatcatttttgatttatatataaatcgttttcattttgtatataaatgattttcaataaatatatagatcgaaaatgatttgtatataaatcacaacttcctaaaggctttttcttgaggggataaaacagaggcacccatcggattttctcttgaatccgacgggtgccttatgtatcaggtgagctatcgaaagactatacggctttactcctcatagaataaaatccgctttgtccgcatgttgcgaccatatatgctgatacgggcaggacacaatagacctgcggccaaacgtgtaatagagtaacggagtacatatctgttttcattgagtcgttagtaaagtattttcgttgataaattagctttctccaaatatatggacatacattcatgagggttttctgccactctgcaaaagtagcaaaaaagctccttcccgacgagtccgattcggacactcgatcaggggtacaaacaaaaatacgaaccaaaaggctttcgctcttagttcgtattcttgagaattgaggtcggttatatagcccgattacgtcgtttcctttgctgaaaatatgccgagatatgactcagccggcgtcttcaaatgttacccaagtctcggtttgttcctgctattacggactgattcggca Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -12.5 Tail_Score: -4.4009 Start: 556 End: 602 Full_Region: atagctcacctgata cataaggcacccgtcggatt caagagaa aatccgatgggtgcctctg ttttatcccctcaag ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cataaggcacccgtcggattcaagagaaaatccgatgggtgcctctg.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -15.3 Tail_Score: -3.80877 Start: 558 End: 599 Full_Region: gctcacctgatacat aaggcacccgtcggatt caagagaa aatccgatgggtgcctc tgttttatcccctca .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................aaggcacccgtcggattcaagagaaaatccgatgggtgcctc....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -16.4 Tail_Score: -3.59698 Start: 560 End: 597 Full_Region: AGGGGATAAAACAGA GGCACCCATCGGATTT TCTCTT GAATCCGACGGGTGCC TTATGTATCAGGTGA ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GGCACCCATCGGATTTTCTCTTGAATCCGACGGGTGCC......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -17.9 Tail_Score: -3.48517 Start: 560 End: 597 Full_Region: tcacctgatacataa ggcacccgtcggatt caagagaa aatccgatgggtgcc tctgttttatcccct ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggcacccgtcggattcaagagaaaatccgatgggtgcc......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -12.5 Tail_Score: -3.88291 Start: 909 End: 953 Full_Region: accgacctcaattct caagaatacgaactaagag cgaaagc cttttggttcgtatttttg tttgtacccctgatc ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................caagaatacgaactaagagcgaaagccttttggttcgtatttttg..................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -7.9 Tail_Score: -5.38054 Start: 915 End: 947 Full_Region: ctcaattctcaagaa tacgaactaagag cgaaagc cttttggttcgta tttttgtttgtaccc ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tacgaactaagagcgaaagccttttggttcgta........................................................................................................................................... TransTerm Strand: + Conf: 51 HP_score: -3.5 Tail_Score: -3.65314 Start: 915 End: 947 Full_Region: GGGTACAAACAAAAA TACGAACCAAAAG GCTTTCG CTCTTAGTTCGTA TTCTTGAGAATTGAG ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TACGAACCAAAAGGCTTTCGCTCTTAGTTCGTA........................................................................................................................................... Find igs database================================================ Query_seq: PG1743:PG1745|PG1743:PG1745:2-dehydro-3-deoxyphosphooctonate aldolase:phosphoribulokinase family protein:<-<-:1828439..1829524 1086 cgtttcgaagttatttatcgtttctgtcagccaaaagtacaaaaagcccttccttaatcaatagagagtagtcgattccgtcgatcggccggccaagagtcgagagggaaaagtgctcagaacaagtaaaaaaatgaccgtcatagctatgcatgaatgaagaacggcaacctgtgttctctttcgttttttgatgagtataaggtaaaagcaaaatctttcgatatgggtcattttcgaagctgaaaaaaacggaatattcaccgaaaaatatgcgccaaaaaagttttcaacaacgaagaagtgtttggaatgattccaaataagcacttctgagagatcgaaattcgaggtaaagtacgtaacatgacagcctttttcagatcgaaaagctctttttattagttcgatgaacaatctatatattaatcatttttgatttatatataaatcgttttcattttgtatataaatgattttcaataaatatatagatcgaaaatgatttgtatataaatcacaacttcctaaaggctttttcttgaggggataaaacagaggcacccatcggattttctcttgaatccgacgggtgccttatgtatcaggtgagctatcgaaagactatacggctttactcctcatagaataaaatccgctttgtccgcatgttgcgaccatatatgctgatacgggcaggacacaatagacctgcggccaaacgtgtaatagagtaacggagtacatatctgttttcattgagtcgttagtaaagtattttcgttgataaattagctttctccaaatatatggacatacattcatgagggttttctgccactctgcaaaagtagcaaaaaagctccttcccgacgagtccgattcggacactcgatcaggggtacaaacaaaaatacgaaccaaaaggctttcgctcttagttcgtattcttgagaattgaggtcggttatatagcccgattacgtcgtttcctttgctgaaaatatgccgagatatgactcagccggcgtcttcaaatgttacccaagtctcggtttgttcctgctattacggactgattcggca Intra-Species Hit: Count: 1 Min: 1 Max: 1086 Len: 1086 Subject: NC_002950_PG1743_PG1745|2-dehydro-3-deoxyphosphooctonate aldolase:phosphoribulokinase family protein|NEGATIVE:NEGATIVE|[1828439,1829524]|1086 HSP 1 e-value: 0.0 bit: 1176.0 Len: 593 Query Start:494 Query End:1086 Subject Strand: POSITIVE Subject Start: 494 Subject End: 1086 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gatcgaaaatgatttgtatataaatcacaacttcctaaaggctttttcttgaggggataaaacagaggcacccatcggattttctcttgaatccgacgggtgccttatgtatcaggtgagctatcgaaagactatacggctttactcctcatagaataaaatccgctttgtccgcatgttgcgaccatatatgctgatacgggcaggacacaatagacctgcggccaaacgtgtaatagagtaacggagtacatatctgttttcattgagtcgttagtaaagtattttcgttgataaattagctttctccaaatatatggacatacattcatgagggttttctgccactctgcaaaagtagcaaaaaagctccttcccgacgagtccgattcggacactcgatcaggggtacaaacaaaaatacgaaccaaaaggctttcgctcttagttcgtattcttgagaattgaggtcggttatatagcccgattacgtcgtttcctttgctgaaaatatgccgagatatgactcagccggcgtcttcaaatgttacccaagtctcggtttgttcctgctattacggactgattcggca .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gatcgaaaatgatttgtatataaatcacaacttcctaaaggctttttcttgaggggataaaacagaggcacccatcggattttctcttgaatccgacgggtgccttatgtatcaggtgagctatcgaaagactatacggctttactcctcatagaataaaatccgctttgtccgcatgttgcgaccatatatgctgatacgggcaggacacaatagacctgcggccaaacgtgtaatagagtaacggagtacatatctgttttcattgagtcgttagtaaagtattttcgttgataaattagctttctccaaatatatggacatacattcatgagggttttctgccactctgcaaaagtagcaaaaaagctccttcccgacgagtccgattcggacactcgatcaggggtacaaacaaaaatacgaaccaaaaggctttcgctcttagttcgtattcttgagaattgaggtcggttatatagcccgattacgtcgtttcctttgctgaaaatatgccgagatatgactcagccggcgtcttcaaatgttacccaagtctcggtttgttcctgctattacggactgattcggca HSP 2 e-value: 0.0 bit: 747.0 Len: 419 Query Start:1 Query End:419 Subject Strand: POSITIVE Subject Start: 1 Subject End: 419 cgtttcgaagttatttatcgtttctgtcagccaaaagtacaaaaagcccttccttaatcaatagagagtagtcgattccgtcgatcggccggccaagagtcgagagggaaaagtgctcagaacaagtnnnnnnntgaccgtcatagctatgcatgaatgaagaacggcaacctgtgttctctttcgttttttgatgagtataaggtaaaagcaaaatctttcgatatgggtcattttcgaagctgnnnnnnncggaatattcaccgaaaaatatgcgccaaaaaagttttcaacaacgaagaagtgtttggaatgattccaaataagcacttctgagagatcgaaattcgaggtaaagtacgtaacatgacagcctttttcagatcgaaaagctctttttattagttcgatgaacaatc........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... cgtttcgaagttatttatcgtttctgtcagccaaaagtacaaaaagcccttccttaatcaatagagagtagtcgattccgtcgatcggccggccaagagtcgagagggaaaagtgctcagaacaagtaaaaaaatgaccgtcatagctatgcatgaatgaagaacggcaacctgtgttctctttcgttttttgatgagtataaggtaaaagcaaaatctttcgatatgggtcattttcgaagctgaaaaaaacggaatattcaccgaaaaatatgcgccaaaaaagttttcaacaacgaagaagtgtttggaatgattccaaataagcacttctgagagatcgaaattcgaggtaaagtacgtaacatgacagcctttttcagatcgaaaagctctttttattagttcgatgaacaatc........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGUUUCGAAGUUAUUUAUCGUUUCUGUCAGCCAAAAGUACAAAAAGCCCUUCCUUAAUCAAUAGAGAGUAGUCGAUUCCGUCGAUCGGCCGGCCAAGAGUCGAGAGGGAAAAGUGCUCAGAACAAGUAAAAAAAUGACCGUCAUAGCUAUGCAUGAAUGAAGAACGGCAACCUGUGUUCUCUUUCGUUUUUUGAUGAGUAUAAGGUAAAAGCAAAAUCUUUCGAUAUGGGUCAUUUUCGAAGCUGAAAAAAACGGAAUAUUCACCGAAAAAUAUGCGCCAAAAAAGUUUUCAACAACGAAGAAGUGUUUGGAAUGAUUCCAAAUAAGCACUUCUGAGAGAUCGAAAUUCGAGGUAAAGUACGUAACAUGACAGCCUUUUUCAGAUCGAAAAGCUCUUUUUAUUAGUUCGAUGAACAAUCUAUAUAUUAAUCAUUUUUGAUUUAUAUAUAAAUCGUUUUCAUUUUGUAUAUAAAUGAUUUUCAAUAAAUAUAUAGAUCGAAAAUGAUUUGUAUAUAAAUCACAACUUCCUAAAGGCUUUUUCUUGAGGGGAUAAAACAGAGGCACCCAUCGGAUUUUCUCUUGAAUCCGACGGGUGCCUUAUGUAUCAGGUGAGCUAUCGAAAGACUAUACGGCUUUACUCCUCAUAGAAUAAAAUCCGCUUUGUCCGCAUGUUGCGACCAUAUAUGCUGAUACGGGCAGGACACAAUAGACCUGCGGCCAAACGUGUAAUAGAGUAACGGAGUACAUAUCUGUUUUCAUUGAGUCGUUAGUAAAGUAUUUUCGUUGAUAAAUUAGCUUUCUCCAAAUAUAUGGACAUACAUUCAUGAGGGUUUUCUGCCACUCUGCAAAAGUAGCAAAAAAGCUCCUUCCCGACGAGUCCGAUUCGGACACUCGAUCAGGGGUACAAACAAAAAUACGAACCAAAAGGCUUUCGCUCUUAGUUCGUAUUCUUGAGAAUUGAGGUCGGUUAUAUAGCCCGAUUACGUCGUUUCCUUUGCUGAAAAUAUGCCGAGAUAUGACUCAGCCGGCGUCUUCAAAUGUUACCCAAGUCUCGGUUUGUUCCUGCUAUUACGGACUGAUUCGGCA ...................(((...(((((((...(((.((.(((((((((.....((((((.((((((((((((((.....))))))).((((.((.(((..((((.....(((((((.........(((((((((...((((.((...))))))....((((((.(.....)))))))...)))))))))..)))))))..((((((.((....((((((((...((((..(((((((..((((((((...((.(((((........)))))...))......(((.(((...(((.(((((((((((((.....)))))....))))))))......(((.....))).........)))....))).))).)))))))).))))))))))).......(((((((.((((((............(((.....(((((((((((((((((((((((..(((((((((...((.....))....)))))))))..)))))))))))))))))))))))......(((.((((.....)))).))).)))......(((((((((.((((((((......)))))))).))))))))).))).)))..))))))))))))))).......))))))))))))...........(((((((((((.((((((.........)))))).)))).)))).))).......)))))..)))).((((..((((.(((.((((((.......)))))))))))))...)))).......)))))))))))))............((((......))))...........))))))))).))..))).......(((((((.....((((((...(((.(.(((((...))))).))))...))))))((((((...(((((((((...(((((....)).))).)))))))))...((((.(((.(((.((((....)))).((..((((((........(((((..((((......))))...)))))))))))..)).......)))))).)))).))))))...)))))))..)).)))))..))). (-301.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGCCGAAUCAGUCCGUAAUAGCAGGAACAAACCGAGACUUGGGUAACAUUUGAAGACGCCGGCUGAGUCAUAUCUCGGCAUAUUUUCAGCAAAGGAAACGACGUAAUCGGGCUAUAUAACCGACCUCAAUUCUCAAGAAUACGAACUAAGAGCGAAAGCCUUUUGGUUCGUAUUUUUGUUUGUACCCCUGAUCGAGUGUCCGAAUCGGACUCGUCGGGAAGGAGCUUUUUUGCUACUUUUGCAGAGUGGCAGAAAACCCUCAUGAAUGUAUGUCCAUAUAUUUGGAGAAAGCUAAUUUAUCAACGAAAAUACUUUACUAACGACUCAAUGAAAACAGAUAUGUACUCCGUUACUCUAUUACACGUUUGGCCGCAGGUCUAUUGUGUCCUGCCCGUAUCAGCAUAUAUGGUCGCAACAUGCGGACAAAGCGGAUUUUAUUCUAUGAGGAGUAAAGCCGUAUAGUCUUUCGAUAGCUCACCUGAUACAUAAGGCACCCGUCGGAUUCAAGAGAAAAUCCGAUGGGUGCCUCUGUUUUAUCCCCUCAAGAAAAAGCCUUUAGGAAGUUGUGAUUUAUAUACAAAUCAUUUUCGAUCUAUAUAUUUAUUGAAAAUCAUUUAUAUACAAAAUGAAAACGAUUUAUAUAUAAAUCAAAAAUGAUUAAUAUAUAGAUUGUUCAUCGAACUAAUAAAAAGAGCUUUUCGAUCUGAAAAAGGCUGUCAUGUUACGUACUUUACCUCGAAUUUCGAUCUCUCAGAAGUGCUUAUUUGGAAUCAUUCCAAACACUUCUUCGUUGUUGAAAACUUUUUUGGCGCAUAUUUUUCGGUGAAUAUUCCGUUUUUUUCAGCUUCGAAAAUGACCCAUAUCGAAAGAUUUUGCUUUUACCUUAUACUCAUCAAAAAACGAAAGAGAACACAGGUUGCCGUUCUUCAUUCAUGCAUAGCUAUGACGGUCAUUUUUUUACUUGUUCUGAGCACUUUUCCCUCUCGACUCUUGGCCGGCCGAUCGACGGAAUCGACUACUCUCUAUUGAUUAAGGAAGGGCUUUUUGUACUUUUGGCUGACAGAAACGAUAAAUAACUUCGAAACG .((((((...(((((((...((((((((((....(((((((((((((..(((((((.....((((((......))))))...)))))))....(....)...((((.(((..((((((.(.(...(((.....((((((((((((((((((((....).)))))))))))))))))))(((((...(((((.(((..(((((...)))))))))))))....((((((((((((((((.....))))))))))))...(((.(((((((((....))))))))))))..)))).........))))).....................)))...).).))))))..)))))))..........)))..))).))))))).)))).))))))((((((......))))))........)))))))...((((.(((((((((....)))))))))))))..((((((((((((((((.(((((......((((((((((((((((........))))))))))))))))..(((((.((.......)).)))))..))))).(((((..(((((((((..(((((((((.(((.(((((((..((((....((((((.......))))))...))))..))))))).))).)))))))))..)))))))))..)......))))........)))).((((((.(((((((((((.((((.....((..........))..(((((((..(..(((((((....((((((.....)))))))))))))..)..))))))).......))))..((((((......))))))...)))))))))))..))))))........))))))))))))..((((((.(((((..............(((.(((((.....((((.(((((..((..((((((...)).))))((((((...........(((..(((.........)))..)))...))))))...))..)))))))))......))))).)))..)))))))))))..........)))))).........(((.........)))..... (-313.33) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1096 120 71 211 162 NC_002950:PG1743|5end_2-dehydro-3-deoxyphosphooctonate_aldolase_1828348..1828578_NEGATIVE 654 330 281 205 156 NC_002950:PG1148|5end_hypothetical_protein_1231204..1231434_POSITIVE 625 319 271 226 178 NC_002950:PG0996|5end_conserved_hypothetical_protein_TIGR01777_1055592..1055822_POSITIVE 516 319 274 230 184 NC_002950:PG1166|5end_hypothetical_protein_1248443..1248673_NEGATIVE 434 608 561 51 1 NC_002950:PG0371|5end_hypothetical_protein_404043..404273_POSITIVE 410 700 655 108 58 NC_002950:PG1006|5end_hypothetical_protein_1066478..1066708_NEGATIVE 386 910 861 169 121 NC_002950:PG0221|5end_hypothetical_protein_255834..256064_NEGATIVE 386 118 81 61 24 NC_002950:PG0146|5end_hypothetical_protein_169115..169345_NEGATIVE 385 117 83 195 166 NC_002950:PG0396|5end_transcriptional_regulator,_Crp/Fnr_family_433513..433743_POSITIVE 381 116 72 169 121 NC_002950:PG0423|5end_hypothetical_protein_462251..462481_POSITIVE 377 910 863 78 16 NC_002950:PG0661|5end_hypothetical_protein_709853..710083_NEGATIVE 374 101 64 162 122 NC_002950:PG1967|5end_TPR_domain_protein_2057168..2057398_POSITIVE 373 110 64 213 164 NC_002950:PG0170|5end_methionyl-tRNA_synthetase_189652..189882_POSITIVE 372 108 63 77 23 NC_002950:PG2075|5end_hypothetical_protein_2176466..2176696_NEGATIVE 372 108 63 77 23 NC_002950:PG1266|5end_hypothetical_protein_1346987..1347217_NEGATIVE 371 119 71 60 6 NC_002950:PG0751|5end_porT_protein_797578..797808_NEGATIVE 369 598 556 78 35 NC_002950:PG1223|5end_hypothetical_protein_1299461..1299691_POSITIVE 369 109 63 216 173 NC_002950:PG0630|5end_pyridoxal_phosphate_biosynthetic_protein_683392..683622_POSITIVE 368 879 831 210 164 NC_002950:PG1866|5end_hypothetical_protein_1964531..1964761_POSITIVE 367 106 66 187 128 NC_002950:PG0809|5end_hypothetical_protein_872014..872244_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 523 599 551 54 2 NC_002950:PG1274|3end_elongation_factor_P_1352489..1352639_POSITIVE 410 700 655 68 18 NC_002950:PG1007|3end_transcriptional_regulator,_GntR_family_1066518..1066668_NEGATIVE 405 607 561 151 102 NC_002950:PG0371|3end_hypothetical_protein_404224..404374_POSITIVE 401 1067 1022 118 74 NC_002950:PG2076|3end_hypothetical_protein_2177073..2177223_POSITIVE 386 910 861 90 42 NC_002950:PG0222|3end_DNA-binding_protein,_histone-like_family_255913..256063_NEGATIVE 386 118 81 48 11 NC_002950:PG0147|3end_hypothetical_protein_169128..169278_NEGATIVE 381 880 834 85 42 NC_002950:PG0340|3end_hypothetical_protein_376436..376586_POSITIVE 375 903 861 145 89 NC_002950:PG0210|3end_precorrin-6x_reductase/cobalamin_biosynthetic_protein_CbiD_244161..244311_NEGATIVE 371 720 674 119 64 NC_002950:PG0604|3end_4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase_661211..661361_POSITIVE 369 116 73 73 21 NC_002950:PG1173|3end_YkgG_family_protein_1253129..1253279_POSITIVE 363 918 871 76 26 NC_002950:PG1585|3end_batD_protein_1664137..1664287_POSITIVE 363 107 62 132 87 NC_002950:PG1497|3end_DNA-binding_protein,_histone-like_family_1574635..1574785_POSITIVE 357 121 86 95 60 NC_002950:PG1975|3end_hemagglutinin_protein_HagC_2064350..2064500_POSITIVE 356 519 471 43 2 NC_002950:PG0773|3end_hypothetical_protein_825348..825498_POSITIVE 355 119 73 95 50 NC_002950:PG1494|3end_hypothetical_protein_1571234..1571384_POSITIVE 355 110 64 64 13 NC_002950:PG0883|3end_hypothetical_protein_946562..946712_POSITIVE 354 619 575 148 95 NC_002950:PG2179|3end_NADH-ubiquinone_oxidoreductase_2287177..2287327_NEGATIVE 352 106 62 75 23 NC_002950:PG1509|3end_HAD-superfamily_hydrolase,_subfamily_IA,_variant_1_family_protein_1587439..1587589_POSITIVE 347 103 76 131 95 NC_002950:PG1213|3end_ribonuclease_H_1293163..1293313_POSITIVE 347 97 63 151 123 NC_002950:PG0143|3end_hydrolase,_carbon-nitrogen_family_165747..165897_NEGATIVE