Origin IGS: aggctttccgacccattgatttcatgctccttccgacctctctcttcacaatttacagccattcctccaacctttttcttttatgataagagagaagggcatcgaaacgtttgtttggtagatactgaaaatggaatcggggctgtgccaatgagattgaacacagccccgaaaatatttggtatttgaacggaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttccgttcaaataccaaatattttcggggctgtgttcaatctcattggcacagccccgattccattttcagtatctaccaaacaaacgtttcgatgcccttctctcttatcataaaagaaaaaggttggaggaatggctgtaaattgtgaagagagaggtcggaaggagcatgaaatcaatgggtcggaaagcct Mask Tandem Repeat Region ================================================ aggctttccgacccattgatttcatgctccttccgacctctctcttcacaatttacagccattcctccaacctttttcttttatgataagagagaagggcatcgaaacgtttgtttggtagatactgaaaatggaatcggggctgtgccaatgagattgaacacagccccgaaaatatttggtatttgaacggaa Find is-nt database================================================ Query_seq: PG1728:PG1729|PG1728:PG1729:cytidine/deoxycytidylate deaminase family protein:thiol peroxidase:-><-:1818237..1818431 195 aggctttccgacccattgatttcatgctccttccgacctctctcttcacaatttacagccattcctccaacctttttcttttatgataagagagaagggcatcgaaacgtttgtttggtagatactgaaaatggaatcggggctgtgccaatgagattgaacacagccccgaaaatatttggtatttgaacggaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1728:PG1729|PG1728:PG1729:cytidine/deoxycytidylate deaminase family protein:thiol peroxidase:-><-:1818237..1818431 195 aggctttccgacccattgatttcatgctccttccgacctctctcttcacaatttacagccattcctccaacctttttcttttatgataagagagaagggcatcgaaacgtttgtttggtagatactgaaaatggaatcggggctgtgccaatgagattgaacacagccccgaaaatatttggtatttgaacggaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1728:PG1729|PG1728:PG1729:cytidine/deoxycytidylate deaminase family protein:thiol peroxidase:-><-:1818237..1818431 195 aggctttccgacccattgatttcatgctccttccgacctctctcttcacaatttacagccattcctccaacctttttcttttatgataagagagaagggcatcgaaacgtttgtttggtagatactgaaaatggaatcggggctgtgccaatgagattgaacacagccccgaaaatatttggtatttgaacggaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aggctttccgacccattgatttcatgctccttccgacctctctcttcacaatttacagccattcctccaacctttttcttttatgataagagagaagggcatcgaaacgtttgtttggtagatactgaaaatggaatcggggctgtgccaatgagattgaacacagccccgaaaatatttggtatttgaacggaa Predict ORF larger than 30AA ================================================ Protein_Len: 57 Strand: + Start: 16 End: 186 ............... M I S C S F R P L S S Q F T A I P P T F F F Y D K R E G H R N V C L V D T E N G I G A V P M R L N T A P K I F G I ......... Protein_Len: 34 Strand: - Start: 84 End: 185 ................................................................................... S L L S P C R F T Q K T S V S F P I P A T G I L N F V A G F I N P M .......... Protein_Len: 41 Strand: - Start: 56 End: 178 ....................................................... L W E E L R K R K H Y S L L A D F R K N P L Y Q F H F R P Q A L S I S C L G S F M ................. aggctttccgacccattgatttcatgctccttccgacctctctcttcacaatttacagccattcctccaacctttttcttttatgataagagagaagggcatcgaaacgtttgtttggtagatactgaaaatggaatcggggctgtgccaatgagattgaacacagccccgaaaatatttggtatttgaacggaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aggctttccgacccattgatttcatgctccttccgacctctctcttcacaatttacagccattcctccaacctttttcttttatgataagagagaagggcatcgaaacgtttgtttggtagatactgaaaatggaatcggggctgtgccaatgagattgaacacagccccgaaaatatttggtatttgaacggaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG1728:PG1729|PG1728:PG1729:cytidine/deoxycytidylate deaminase family protein:thiol peroxidase:-><-:1818237..1818431 195 aggctttccgacccattgatttcatgctccttccgacctctctcttcacaatttacagccattcctccaacctttttcttttatgataagagagaagggcatcgaaacgtttgtttggtagatactgaaaatggaatcggggctgtgccaatgagattgaacacagccccgaaaatatttggtatttgaacggaa Intra-Species Hit: Count: 1 Min: 1 Max: 195 Len: 195 Subject: NC_002950_PG1728_PG1729|cytidine/deoxycytidylate deaminase family protein:thiol peroxidase|POSITIVE:NEGATIVE|[1818237,1818431]|195 HSP 1 e-value: 1.0E-106 bit: 387.0 Len: 195 Query Start:1 Query End:195 Subject Strand: POSITIVE Subject Start: 1 Subject End: 195 aggctttccgacccattgatttcatgctccttccgacctctctcttcacaatttacagccattcctccaacctttttcttttatgataagagagaagggcatcgaaacgtttgtttggtagatactgaaaatggaatcggggctgtgccaatgagattgaacacagccccgaaaatatttggtatttgaacggaa aggctttccgacccattgatttcatgctccttccgacctctctcttcacaatttacagccattcctccaacctttttcttttatgataagagagaagggcatcgaaacgtttgtttggtagatactgaaaatggaatcggggctgtgccaatgagattgaacacagccccgaaaatatttggtatttgaacggaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGGCUUUCCGACCCAUUGAUUUCAUGCUCCUUCCGACCUCUCUCUUCACAAUUUACAGCCAUUCCUCCAACCUUUUUCUUUUAUGAUAAGAGAGAAGGGCAUCGAAACGUUUGUUUGGUAGAUACUGAAAAUGGAAUCGGGGCUGUGCCAAUGAGAUUGAACACAGCCCCGAAAAUAUUUGGUAUUUGAACGGAA .....(((((..(((.((.((((((((.(((((.....(((((..(((.((.............................)).)))..)))))))))))))).))))))......)))(((((((..((.((....(((((((((((.((((...))))..)))))))))))..)).))..)))))))..))))) (-54.75) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCCGUUCAAAUACCAAAUAUUUUCGGGGCUGUGUUCAAUCUCAUUGGCACAGCCCCGAUUCCAUUUUCAGUAUCUACCAAACAAACGUUUCGAUGCCCUUCUCUCUUAUCAUAAAAGAAAAAGGUUGGAGGAAUGGCUGUAAAUUGUGAAGAGAGAGGUCGGAAGGAGCAUGAAAUCAAUGGGUCGGAAAGCCU (((((((((.((((.((((....((((((((((((.((((...))))))))))))))))....))))...)))).............(((((.(((((((((((((((.((((((........(((((......))))).....)))))).)))))).....))))).)))))))))...)))).)))))..... (-59.72) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 414 176 136 79 39 NC_002950:PG1005|5end_lipoprotein,_putative_1063763..1063993_NEGATIVE 381 169 128 225 182 NC_002950:PG0749|5end_hypothetical_protein_795654..795884_NEGATIVE 364 169 124 64 17 NC_002950:PG2175|5end_hypothetical_protein_2283116..2283346_NEGATIVE 328 150 104 146 96 NC_002950:PG0648|5end_iron_compound_ABC_transporter,_periplasmic_iron_compound-binding_protein,_putative_701285..701515_NEGATIVE 327 171 131 85 44 NC_002950:PG2019|5end_hypothetical_protein_2111662..2111892_NEGATIVE 326 160 112 215 162 NC_002950:PG1102|5end_hypothetical_protein_1173637..1173867_NEGATIVE 320 170 123 174 123 NC_002950:PG0480|5end_precorrin-2_C20-methyltransferase,_putative_521319..521549_NEGATIVE 319 170 125 191 144 NC_002950:PG0095|5end_DNA_mismatch_repair_protein_114763..114993_NEGATIVE 314 180 131 226 169 NC_002950:PG1739|5end_hypothetical_protein_1825709..1825939_NEGATIVE 311 149 103 202 151 NC_002950:PG0072|5end_UDP-3-O-[3-hydroxymyristoyl]_glucosamine_N-acyltransferase_87147..87377_NEGATIVE 310 170 125 89 49 NC_002950:PG0524|5end_hypothetical_protein_568599..568829_POSITIVE 309 46 2 82 38 NC_002950:PG1391|5end_hypothetical_protein_1471670..1471900_POSITIVE 309 172 131 124 74 NC_002950:PG0287|5end_hypothetical_protein_320457..320687_POSITIVE 305 48 12 191 159 NC_002950:PG2035|5end_tRNA_(guanine-N(1)-)-methyltransferase_2135852..2136082_POSITIVE 300 147 107 58 5 NC_002950:PG1160|5end_L-threonine-O-3-phosphate_decarboxylase,_putative_1239988..1240218_NEGATIVE 299 170 139 55 21 NC_002950:PG1234|5end_hypothetical_protein_1310112..1310342_POSITIVE 299 149 102 159 94 NC_002950:PG0664|5end_oxidoreductase,_Gfo/Idh/MocA_family_710293..710523_POSITIVE 298 170 131 150 112 NC_002950:PG2142|5end_GTP-binding_protein_Era_2247585..2247815_POSITIVE 296 99 53 137 99 NC_002950:PG0706|5end_hypothetical_protein_760350..760580_POSITIVE 292 169 121 192 150 NC_002950:PG1633|5end_galactokinase_1713385..1713615_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 414 176 136 48 8 NC_002950:PG1006|3end_hypothetical_protein_1063794..1063944_NEGATIVE 412 173 139 44 7 NC_002950:PG1950|3end_hypothetical_protein_2040840..2040990_POSITIVE 405 69 24 76 23 NC_002950:PG2037|3end_hypothetical_protein_2137591..2137741_NEGATIVE 405 172 131 65 9 NC_002950:PG0548|3end_pyruvate_ferredoxin/flavodoxin_oxidoreductase_family_protein_605759..605909_POSITIVE 391 183 142 51 1 NC_002950:PG1972|3end_hemagglutinin_protein_HagB_2061203..2061353_POSITIVE 360 171 133 49 7 NC_002950:PG2124|3end_glyceraldehyde_3-phosphate_dehydrogenase,_type_I_2231264..2231414_POSITIVE 348 170 133 61 22 NC_002950:PG0034|3end_thioredoxin_42235..42385_NEGATIVE 344 169 130 57 10 NC_002950:PG0349|3end_hydrolase,_haloacid_dehalogenase-like_family_385291..385441_POSITIVE 336 170 132 44 9 NC_002950:PG1809|3end_2-oxoglutarate_oxidoreductase,_gamma_subunit_1905491..1905641_NEGATIVE 331 169 135 67 30 NC_002950:PG0506|3end_arginine-specific_cysteine_proteinase_547625..547775_POSITIVE 330 169 122 54 2 NC_002950:PG0435|3end_capsular_polysaccharide_biosythesis_protein,_putative_472708..472858_NEGATIVE 328 170 125 49 2 NC_002950:PG1754|3end_hypothetical_protein_1842284..1842434_POSITIVE 327 171 131 139 98 NC_002950:PG2020|3end_CRISPR-associated_TM1814_family_protein_2111608..2111758_NEGATIVE 327 168 121 70 18 NC_002950:PG1757|3end_hypothetical_protein_1843931..1844081_NEGATIVE 327 149 101 114 70 NC_002950:PG0237|3end_uracil-DNA_glycosylase_275526..275676_POSITIVE 324 168 126 52 5 NC_002950:PG2170|3end_sugar_transporter_2276685..2276835_NEGATIVE 324 184 141 66 16 NC_002950:PG1186|3end_hypothetical_protein_1267703..1267853_NEGATIVE 322 169 121 86 39 NC_002950:PG0725|3end_hydrolase,_haloacid_dehalogenase-like_family_777966..778116_POSITIVE 320 170 123 61 10 NC_002950:PG0481|3end_2-amino-3-ketobutyrate_coenzyme_A_ligase_521432..521582_NEGATIVE 319 170 126 62 19 NC_002950:PG0186|3end_lipoprotein_RagB_222997..223147_POSITIVE