Origin IGS: tattatatgttggattattaaaattagtcagctacctatttggaacgtttttttgtctttgatattgtttggtctgctgcagtgccttcttcgagatgtatcaggcggcgaaagagtttgggcaaaggcaacttatattcctgcgtatctcccgtttcaggatgacggaatacgaatcgcgttccctgtagggccagacagtccagatggcggaaagaactaccctgcctccaatcacctgcaatcggaatacccaactgccccatttgccgtcgaagctgattgttccgaccgctcagcaaagtcacttccacgagcgagcataccgctccgcgacgaataaccttgtaacgagccgttcccaccgaatgaacggaaggagtctcagccacgatacgagactgcttgcccttgatcgaggtttgataccgaggaggatccagcgtaccctcggcatcgggcatctctccttcgatcgccatcacgaaacgctgttccaatacgaccttggaccacttgtccaccacaaacccctgtacctctttattcttggcaaagagcaccattccggcagaatctttgtccagtctgttgagcataaagattttcacctccggatcatattgcttgagatgatcgctaacgatgcgcagagcagtcctgtccttattgacgccactggctacagtggagactccggctttcttctcgatcaggacgaaatagtcgtcctgccaaatgatccgcaccaaaggatggcgcagctcttccggtagctgtgtccgatgcacgattaccctgtcgccggctttcagctctgcatcgtgtcgagtagtcggcacaccgttgatggaaacttgccgtctcgtcaatatctgcttaacggacgtgcggctattatccttcagcatacgaacgagaaaaggcaagagggtatcctcttctctgacttcgaaaaccttgtccggctgacgtctcaggcggggtgaccgactttttggaggttgcatatttcgtttgtagagaatc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gattctctacaaacgaaatatgcaacctccaaaaagtcggtcaccccgcctgagacgtcagccggacaaggttttcgaagtcagagaagaggataccctcttgccttttctcgttcgtatgctgaaggataatagccgcacgtccgttaagcagatattgacgagacggcaagtttccatcaacggtgtgccgactactcgacacgatgcagagctgaaagccggcgacagggtaatcgtgcatcggacacagctaccggaagagctgcgccatcctttggtgcggatcatttggcaggacgactatttcgtcctgatcgagaagaaagccggagtctccactgtagccagtggcgtcaataaggacaggactgctctgcgcatcgttagcgatcatctcaagcaatatgatccggaggtgaaaatctttatgctcaacagactggacaaagattctgccggaatggtgctctttgccaagaataaagaggtacaggggtttgtggtggacaagtggtccaaggtcgtattggaacagcgtttcgtgatggcgatcgaaggagagatgcccgatgccgagggtacgctggatcctcctcggtatcaaacctcgatcaagggcaagcagtctcgtatcgtggctgagactccttccgttcattcggtgggaacggctcgttacaaggttattcgtcgcggagcggtatgctcgctcgtggaagtgactttgctgagcggtcggaacaatcagcttcgacggcaaatggggcagttgggtattccgattgcaggtgattggaggcagggtagttctttccgccatctggactgtctggccctacagggaacgcgattcgtattccgtcatcctgaaacgggagatacgcaggaatataagttgcctttgcccaaactctttcgccgcctgatacatctcgaagaaggcactgcagcagaccaaacaatatcaaagacaaaaaaacgttccaaataggtagctgactaattttaataatccaacatataata Mask Tandem Repeat Region ================================================ tattatatgttggattattaaaattagtcagctacctatttggaacgtttttttgtctttgatattgtttggtctgctgcagtgccttcttcgagatgtatcaggcggcgaaagagtttgggcaaaggcaacttatattcctgcgtatctcccgtttcaggatgacggaatacgaatcgcgttccctgtagggccagacagtccagatggcggaaagaactaccctgcctccaatcacctgcaatcggaatacccaactgccccatttgccgtcgaagctgattgttccgaccgctcagcaaagtcacttccacgagcgagcataccgctccgcgacgaataaccttgtaacgagccgttcccaccgaatgaacggaaggagtctcagccacgatacgagactgcttgcccttgatcgaggtttgataccgaggaggatccagcgtaccctcggcatcgggcatctctccttcgatcgccatcacgaaacgctgttccaatacgaccttggaccacttgtccaccacaaacccctgtacctctttattcttggcaaagagcaccattccggcagaatctttgtccagtctgttgagcataaagattttcacctccggatcatattgcttgagatgatcgctaacgatgcgcagagcagtcctgtccttattgacgccactggctacagtggagactccggctttcttctcgatcaggacgaaatagtcgtcctgccaaatgatccgcaccaaaggatggcgcagctcttccggtagctgtgtccgatgcacgattaccctgtcgccggctttcagctctgcatcgtgtcgagtagtcggcacaccgttgatggaaacttgccgtctcgtcaatatctgcttaacggacgtgcggctattatccttcagcatacgaacgagaaaaggcaagagggtatcctcttctctgacttcgaaaaccttgtccggctgacgtctcaggcggggtgaccgactttttggaggttgcatatttcgtttgtagagaatc Find is-nt database================================================ Query_seq: PG1704:PG1706|PG1704:PG1706:thiol:disulfide interchange protein dsbD, putative:hypothetical protein:<-<-:1792910..1793938 1029 tattatatgttggattattaaaattagtcagctacctatttggaacgtttttttgtctttgatattgtttggtctgctgcagtgccttcttcgagatgtatcaggcggcgaaagagtttgggcaaaggcaacttatattcctgcgtatctcccgtttcaggatgacggaatacgaatcgcgttccctgtagggccagacagtccagatggcggaaagaactaccctgcctccaatcacctgcaatcggaatacccaactgccccatttgccgtcgaagctgattgttccgaccgctcagcaaagtcacttccacgagcgagcataccgctccgcgacgaataaccttgtaacgagccgttcccaccgaatgaacggaaggagtctcagccacgatacgagactgcttgcccttgatcgaggtttgataccgaggaggatccagcgtaccctcggcatcgggcatctctccttcgatcgccatcacgaaacgctgttccaatacgaccttggaccacttgtccaccacaaacccctgtacctctttattcttggcaaagagcaccattccggcagaatctttgtccagtctgttgagcataaagattttcacctccggatcatattgcttgagatgatcgctaacgatgcgcagagcagtcctgtccttattgacgccactggctacagtggagactccggctttcttctcgatcaggacgaaatagtcgtcctgccaaatgatccgcaccaaaggatggcgcagctcttccggtagctgtgtccgatgcacgattaccctgtcgccggctttcagctctgcatcgtgtcgagtagtcggcacaccgttgatggaaacttgccgtctcgtcaatatctgcttaacggacgtgcggctattatccttcagcatacgaacgagaaaaggcaagagggtatcctcttctctgacttcgaaaaccttgtccggctgacgtctcaggcggggtgaccgactttttggaggttgcatatttcgtttgtagagaatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1704:PG1706|PG1704:PG1706:thiol:disulfide interchange protein dsbD, putative:hypothetical protein:<-<-:1792910..1793938 1029 tattatatgttggattattaaaattagtcagctacctatttggaacgtttttttgtctttgatattgtttggtctgctgcagtgccttcttcgagatgtatcaggcggcgaaagagtttgggcaaaggcaacttatattcctgcgtatctcccgtttcaggatgacggaatacgaatcgcgttccctgtagggccagacagtccagatggcggaaagaactaccctgcctccaatcacctgcaatcggaatacccaactgccccatttgccgtcgaagctgattgttccgaccgctcagcaaagtcacttccacgagcgagcataccgctccgcgacgaataaccttgtaacgagccgttcccaccgaatgaacggaaggagtctcagccacgatacgagactgcttgcccttgatcgaggtttgataccgaggaggatccagcgtaccctcggcatcgggcatctctccttcgatcgccatcacgaaacgctgttccaatacgaccttggaccacttgtccaccacaaacccctgtacctctttattcttggcaaagagcaccattccggcagaatctttgtccagtctgttgagcataaagattttcacctccggatcatattgcttgagatgatcgctaacgatgcgcagagcagtcctgtccttattgacgccactggctacagtggagactccggctttcttctcgatcaggacgaaatagtcgtcctgccaaatgatccgcaccaaaggatggcgcagctcttccggtagctgtgtccgatgcacgattaccctgtcgccggctttcagctctgcatcgtgtcgagtagtcggcacaccgttgatggaaacttgccgtctcgtcaatatctgcttaacggacgtgcggctattatccttcagcatacgaacgagaaaaggcaagagggtatcctcttctctgacttcgaaaaccttgtccggctgacgtctcaggcggggtgaccgactttttggaggttgcatatttcgtttgtagagaatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 4 Min: 93 Max: 923 Len: 831 Subject: gi|89109396|ref|AP_003176.1| 23S rRNA pseudouridine synthase [Escherichia coli W3110] HSP 1 e-value: 2.0E-15 bit: 80.1 Len: 828 Query Start:93 Query End:920 Subject Strand: null Subject Start: 24 Subject End: 311 ............................................................................................ L V R M L K D N S R T S V K Q I L T R R Q V S I N G V P T T R H D A E L K A G D R V I V H R T Q L P E E L R H - - - - - P L V R I I W Q D D Y F V L I E K K A G V S T V A S G V N K D R T A L R I V S D H L K Q Y D P E V K I F M L N R L D K D S A G M V L F A K N K E V Q G F V V D K W S K V V L E Q R F V M A I E G E M P D A E G T L D P P R Y Q T S I K G K Q S R I V A E T P S V H S V G - - - T A R Y K V I R R G A V C S L V E V T L L S G R N N Q L R R Q M G Q L G I P I A G D W R Q G - - - - - - - - - - - - - - S S F R H L D C L A L Q G T R F V F R H P E T G D T Q E Y K L P L P K L F R R L I H L ............................................................................................................. ............................................................................................ L A E M F P D Y S R S R I K E W I L D Q R V L V N G K V C D K P K E K V L G G E Q V A I N - A E I E E E A R F E P Q D I P L - D I V Y E D E D I I I I N K P R D L V V H P G A G N P D G T V L N A L L H Y Y P P I A D V P R A G I V H R L D K D T T G L M V V A K T V P A Q T R L V E S L Q R R E I T R E Y E A V A I G H M - T A G G T V D E P I S R H P T K R T H M - - - - - - - A V H P M G K P A V T H Y R I M E H F R V H T R L R L R L E T G R T H Q I R V H M A H I T H P L V G D P V Y G G R P R P P K G A S E A F I S T L R K F D R Q A L H A T M L R L Y H P I S G I E M E W H A P I P Q D M V E L I E V ............................................................................................................. Subject: gi|89107932|ref|AP_001712.1| 23S rRNA pseudouridylate synthase [Escherichia coli W3110] HSP 1 e-value: 2.0E-12 bit: 70.5 Len: 825 Query Start:99 Query End:923 Subject Strand: null Subject Start: 25 Subject End: 312 .................................................................................................. F L V R M L K D N S R T S V K Q I L T R R Q V S I N G V P T T R H D A E L K A G D R V I V H R T Q L P E - - - E L R H P L V R - - - - - - - - I I W Q D D Y F V L I E K K A G V - - - - - S T V A S G V N K D R T A L R I V S D H L K Q Y D P E V K I F - M L N R L D K D S A G M V L F A K N K E V Q G F V V D K W S K V V L E Q R F V M A I E G E M P D A E G T L D P P R Y Q T S I K G K Q S R I V A E T P S V H S V G T A R Y K V I R R G A V C S L V E V T L L S G R N N Q L R R Q M G Q L G I P I A G D W R Q G - - - - - - - - - S S F R H L D C L A L Q G T R F V F R H P E T G D T Q E Y K L P L P K L F R R L I .......................................................................................................... .................................................................................................. F L R T Q L K G V P K S M I Y R I L R K G E V R V N K - K R I K P E Y K L E A G D E V R I P P V R V A E R E E E A V S P H L Q K V A A L A D V I L Y E D D H I L V L N K P S G T A V H G G S G L S F G V I E G L R A L R - - - - - - - - - - P E A R F L E L V H R L D R D T S G V L L V A K K R S A L R S L H E Q L R E K G M Q K D Y L A L V R G Q W Q S H V K S V Q A P L L K N I L Q S G E - R I V R V S Q E G K P S E T - R F K V E E R Y A F A T L V R C S P V T G R T H Q I R V H T Q Y A G H P I A F D D R Y G D R E F D R Q L T E A G T G L N R L F L H A A A L K F T H P G T G E V M R I E A P M D E G L K R C L .......................................................................................................... Subject: gi|89109577|ref|AP_003357.1| tRNA pseudouridine synthase [Escherichia coli W3110] HSP 1 e-value: 2.0E-11 bit: 66.6 Len: 606 Query Start:147 Query End:752 Subject Strand: null Subject Start: 1 Subject End: 215 .................................................................................................................................................. L V R I I W Q D D Y F V L I E K K A G V S T V A S G V N K D R - - T A L R I V S D H L K Q Y D P E V K I F M L N R L D K D S A G M V L F A K N K E V Q G F V V D K W S K V V L E Q R F V M A I E G E M P D A E G T L D P P - - - - - - - - - - R Y Q T S I K G K Q S R I V - - - - - - - A E T P S V H S V G T A R Y K V I R R G A V C S L V E V T L L S G R N N Q L R R Q M G Q L G I P I A G D W R Q G - - - - - - S S F R H - - L D C L A L Q G T R F V F R H P E T G D ..................................................................................................................................................................................................................................................................................... .................................................................................................................................................. M L E I L Y Q D E W L V A V N K P S G W L V H R S W L D R D E K V V V M Q T V R D Q I G Q H - - - - - V F T A H R L D R P T S G V L L M G L S S E A G R L L A Q Q F E Q H Q I Q K R Y H A I V R G W L M E - E A V L D Y P L V E E L D K I A D K F A R E D K G P Q P A V T H Y R G L A T V E M P - - - - V A T G R Y P T T R Y G - - - - L V E L E P K T G R K H Q L R R H L A H L R H P I I G D S K H G D L R Q N R S G A E H F G L Q R L M L H A S Q L S L T H P F T G E ..................................................................................................................................................................................................................................................................................... Subject: gi|89106942|ref|AP_000722.1| pseudouridine synthase for 23S rRNA (position 746) and tRNAphe(position 32) [Escherichia coli W3110] HSP 1 e-value: 6.0E-9 bit: 58.5 Len: 636 Query Start:126 Query End:761 Subject Strand: null Subject Start: 10 Subject End: 216 ............................................................................................................................. R H P L V R I I W Q D D Y F V L I E K K A G V S T V A S G V - - N K D R T A L R I V S D H L K Q Y D P E V K I F M L N R L D K D S A G M V L F A K N K E V Q G F V V D K W S K V V L E Q R F V M A I E G E M P D A E G T L D P P R Y Q T S I K G K Q S R I V A E T P S V H S V G T A R Y K V I R R G A V - C S L V E V T L L S G R N N Q L R R Q M G Q L G I P I A G D - - W R Q G S S F R H L D C L A L Q G T R F V F R H P E T G D T Q E Y K L P ............................................................................................................................................................................................................................................................................ ............................................................................................................................. Q E P W L V I L Y Q D D H I M V V N K P S G L L S V P G R L E E H K D S V M T R I Q R D Y - - - - - P Q A E - - S V H R L D M A T S G V I V V A L T K A A E R E L K R Q F R E R E P K K Q Y V A R V W G H P S P A E G L V D L P L I C D W P N R P K Q K V C Y E T G K P - - - A Q T E Y E V V E Y A A D N T A R V V L K P I T G R S H Q L R V H M L A L G H P I L G D R F Y A S P E A R A M A P R L L L H A E M L T I T H P A Y G N S M T F K A P ............................................................................................................................................................................................................................................................................ Find nr database================================================ Query_seq: PG1704:PG1706|PG1704:PG1706:thiol:disulfide interchange protein dsbD, putative:hypothetical protein:<-<-:1792910..1793938 1029 tattatatgttggattattaaaattagtcagctacctatttggaacgtttttttgtctttgatattgtttggtctgctgcagtgccttcttcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaggcaagagggtatcctcttctctgacttcgaaaaccttgtccggctgacgtctcaggcggggtgaccgactttttggaggttgcatatttcgtttgtagagaatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tattatatgttggattattaaaattagtcagctacctatttggaacgtttttttgtctttgatattgtttggtctgctgcagtgccttcttcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaggcaagagggtatcctcttctctgacttcgaaaaccttgtccggctgacgtctcaggcggggtgaccgactttttggaggttgcatatttcgtttgtagagaatc Predict ORF larger than 30AA ================================================ Protein_Len: 41 Strand: + Start: 40 End: 162 ....................................... M E R F F V F D I V W S A A V P S S R C I R R R K S L G K G N L Y S C V S P V S G ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 40 Strand: + Start: 69 End: 188 .................................................................... M V C C S A F F E M Y Q A A K E F G Q R Q L I F L R I S R F R M T E Y E S R S L ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 2 End: 340 . M I C W I I K I S Q L P I W N V F L S L I L F G L L Q C L L R D V S G G E R V W A K A T Y I P A Y L P F Q D D G I R I A ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 31 Strand: + Start: 500 End: 592 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M R P W T T C P P Q T P V P L Y S W Q R A P F R Q N L C P V C ..................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 57 Strand: + Start: 463 End: 633 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M S P S I A I T K R C S N T T L D H L S T T N P C T S L F L A K S T I P A E S L S S L L S I K I F T S G S Y C L R ............................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 207 End: 773 .............................................................................................................................................................................................................. M A E R T T L P P I T C N R N T Q L P H L P S K L I V P T A Q Q S H F H E R A Y R S A T N N L V T S R S H R M N G R S L ................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 739 End: 969 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M I R T K G W R S S S G S C V R C T I T L S P A F S S A S C R V V G T P L M E T C R L V N I C L T D V R L L S F S I R T ............................................................ Protein_Len: 60 Strand: + Start: 777 End: 986 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M C P M H D Y P V A G F Q L C I V S S S R H T V D G N L P S R Q Y L L N G R A A I I L Q H T N E K R Q E G I L F S D F E ........................................... Protein_Len: 60 Strand: - Start: 670 End: 873 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. S R S E E R D P R F L R G A L H D A G F S P A A R G T A T D S A R N G Q R R S E A R C R T S Y D A C R Q H F S A T E D M ............................................................................................................................................................ Protein_Len: 31 Strand: - Start: 485 End: 577 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... S V S N W Y S R P G S T W W L G R Y R K I R P L S C W E P L M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 43 Strand: - Start: 388 End: 516 ................................................................................................................................................................................................................................................................................................................................................................................................... G R Y S V A Q G Q D L N S V S S S G A Y G R C R A D R R R D G D R F A T G I R G Q V M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 50 Strand: - Start: 308 End: 457 ................................................................................................................................................................................................................................................................................................................... K W S R A Y R E A V F L R T V L R E W R I F P L L R L W S V L S S A R S R P K I G L L I W R V R P M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: - Start: 104 End: 283 ....................................................................................................... A A F S N P C L C S I N R R I E R K L I V S Y S D R E R Y P W V T W I A S L V V R G G I V Q L R F V W S G W K G D F S M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 39 End: 1028 ...................................... I S V Q R R T L I Q K V S T R S N D K L M R V L F P L L T D E E R V E F V K D P Q R R L R P S R S K P P Q M N R K Y L M . tattatatgttggattattaaaattagtcagctacctatttggaacgtttttttgtctttgatattgtttggtctgctgcagtgccttcttcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaggcaagagggtatcctcttctctgacttcgaaaaccttgtccggctgacgtctcaggcggggtgaccgactttttggaggttgcatatttcgtttgtagagaatc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tattatatgttggattattaaaattagtcagctacctatttggaacgtttttttgtctttgatattgtttggtctgctgcagtgccttcttcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaggcaagagggtatcctcttctctgacttcgaaaaccttgtccggctgacgtctcaggcggggtgaccgactttttggaggttgcatatttcgtttgtagagaatc Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 38 HP_score: -2.0 Tail_Score: -2.57132 Start: 493 End: 515 Full_Region: GCCATCACGAAACGC TGTTCCAA TACGACC TTGGACCA CTTGTCCACCACAAA ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGTTCCAATACGACCTTGGACCA.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 49 HP_score: -3.0 Tail_Score: -3.53091 Start: 740 End: 768 Full_Region: gacacagctaccgga agagctgcgcca tcctt tggtgcggatca tttggcaggacgact ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agagctgcgccatcctttggtgcggatca..................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 51 HP_score: -6.5 Tail_Score: -2.71712 Start: 741 End: 767 Full_Region: acacagctaccggaa gagctgcgcca tcctt tggtgcggatc atttggcaggacgac ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gagctgcgccatcctttggtgcggatc...................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: PG1704:PG1706|PG1704:PG1706:thiol:disulfide interchange protein dsbD, putative:hypothetical protein:<-<-:1792910..1793938 1029 tattatatgttggattattaaaattagtcagctacctatttggaacgtttttttgtctttgatattgtttggtctgctgcagtgccttcttcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaggcaagagggtatcctcttctctgacttcgaaaaccttgtccggctgacgtctcaggcggggtgaccgactttttggaggttgcatatttcgtttgtagagaatc Intra-Species Hit: Count: 1 Min: 1 Max: 1029 Len: 1029 Subject: NC_002950_PG1704_PG1706|thiol:disulfide interchange protein dsbD, putative:hypothetical protein|NEGATIVE:NEGATIVE|[1792910,1793938]|1029 HSP 1 e-value: 7.0E-53 bit: 210.0 Len: 106 Query Start:924 Query End:1029 Subject Strand: POSITIVE Subject Start: 924 Subject End: 1029 ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................aggcaagagggtatcctcttctctgacttcgaaaaccttgtccggctgacgtctcaggcggggtgaccgactttttggaggttgcatatttcgtttgtagagaatc ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................aggcaagagggtatcctcttctctgacttcgaaaaccttgtccggctgacgtctcaggcggggtgaccgactttttggaggttgcatatttcgtttgtagagaatc HSP 2 e-value: 2.0E-44 bit: 182.0 Len: 92 Query Start:1 Query End:92 Subject Strand: POSITIVE Subject Start: 1 Subject End: 92 tattatatgttggattattaaaattagtcagctacctatttggaacgtttttttgtctttgatattgtttggtctgctgcagtgccttcttc......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... tattatatgttggattattaaaattagtcagctacctatttggaacgtttttttgtctttgatattgtttggtctgctgcagtgccttcttc......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUUAUAUGUUGGAUUAUUAAAAUUAGUCAGCUACCUAUUUGGAACGUUUUUUUGUCUUUGAUAUUGUUUGGUCUGCUGCAGUGCCUUCUUCGAGAUGUAUCAGGCGGCGAAAGAGUUUGGGCAAAGGCAACUUAUAUUCCUGCGUAUCUCCCGUUUCAGGAUGACGGAAUACGAAUCGCGUUCCCUGUAGGGCCAGACAGUCCAGAUGGCGGAAAGAACUACCCUGCCUCCAAUCACCUGCAAUCGGAAUACCCAACUGCCCCAUUUGCCGUCGAAGCUGAUUGUUCCGACCGCUCAGCAAAGUCACUUCCACGAGCGAGCAUACCGCUCCGCGACGAAUAACCUUGUAACGAGCCGUUCCCACCGAAUGAACGGAAGGAGUCUCAGCCACGAUACGAGACUGCUUGCCCUUGAUCGAGGUUUGAUACCGAGGAGGAUCCAGCGUACCCUCGGCAUCGGGCAUCUCUCCUUCGAUCGCCAUCACGAAACGCUGUUCCAAUACGACCUUGGACCACUUGUCCACCACAAACCCCUGUACCUCUUUAUUCUUGGCAAAGAGCACCAUUCCGGCAGAAUCUUUGUCCAGUCUGUUGAGCAUAAAGAUUUUCACCUCCGGAUCAUAUUGCUUGAGAUGAUCGCUAACGAUGCGCAGAGCAGUCCUGUCCUUAUUGACGCCACUGGCUACAGUGGAGACUCCGGCUUUCUUCUCGAUCAGGACGAAAUAGUCGUCCUGCCAAAUGAUCCGCACCAAAGGAUGGCGCAGCUCUUCCGGUAGCUGUGUCCGAUGCACGAUUACCCUGUCGCCGGCUUUCAGCUCUGCAUCGUGUCGAGUAGUCGGCACACCGUUGAUGGAAACUUGCCGUCUCGUCAAUAUCUGCUUAACGGACGUGCGGCUAUUAUCCUUCAGCAUACGAACGAGAAAAGGCAAGAGGGUAUCCUCUUCUCUGACUUCGAAAACCUUGUCCGGCUGACGUCUCAGGCGGGGUGACCGACUUUUUGGAGGUUGCAUAUUUCGUUUGUAGAGAAUC ........((((((..........(((((((((.(((((..(((((((....(((((((((..(((.(((.(.(((((...((((..((.....)).))))..)))))).))).))).....)))))))))............(((((...(((((.......))))).)))))....)))))))..)))))....(((....(((((((.((............(((............)))...((.....)).....)))))))))..)))..))))))))).)))))).((((((((..((((......((.((((((.....))).)))..))...(((((((...((((((((((.........))))))(((.((((((...........)))))).)))...))))..)))))))))))...((((.(((..((((((....(((......((((((........))).)))....))).)))))).)))..........(((((.....)))))...............)))).......((((((((((...((.....)).....)))))).))))..)))))))).....(((((((.((((((((.......((((((((((.(((...(((((((..(((((.(((..(((......))).((((((....)))))).))).(((((............((((((((.....)))))))).....(((((.(((.....((...(((((((((.....).))))))))))..)))..))))).......)))))......))))))))))))..))).(((((((.(((..(((((.(((.....(((((..((((..((......))...)))).))))).....))).))))).......((((...(((.((((((....)))))).)))...)))).......)))))))))).)))))))))).((....)).....)))))))).((((.........))))))))))) (-329.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUUCUCUACAAACGAAAUAUGCAACCUCCAAAAAGUCGGUCACCCCGCCUGAGACGUCAGCCGGACAAGGUUUUCGAAGUCAGAGAAGAGGAUACCCUCUUGCCUUUUCUCGUUCGUAUGCUGAAGGAUAAUAGCCGCACGUCCGUUAAGCAGAUAUUGACGAGACGGCAAGUUUCCAUCAACGGUGUGCCGACUACUCGACACGAUGCAGAGCUGAAAGCCGGCGACAGGGUAAUCGUGCAUCGGACACAGCUACCGGAAGAGCUGCGCCAUCCUUUGGUGCGGAUCAUUUGGCAGGACGACUAUUUCGUCCUGAUCGAGAAGAAAGCCGGAGUCUCCACUGUAGCCAGUGGCGUCAAUAAGGACAGGACUGCUCUGCGCAUCGUUAGCGAUCAUCUCAAGCAAUAUGAUCCGGAGGUGAAAAUCUUUAUGCUCAACAGACUGGACAAAGAUUCUGCCGGAAUGGUGCUCUUUGCCAAGAAUAAAGAGGUACAGGGGUUUGUGGUGGACAAGUGGUCCAAGGUCGUAUUGGAACAGCGUUUCGUGAUGGCGAUCGAAGGAGAGAUGCCCGAUGCCGAGGGUACGCUGGAUCCUCCUCGGUAUCAAACCUCGAUCAAGGGCAAGCAGUCUCGUAUCGUGGCUGAGACUCCUUCCGUUCAUUCGGUGGGAACGGCUCGUUACAAGGUUAUUCGUCGCGGAGCGGUAUGCUCGCUCGUGGAAGUGACUUUGCUGAGCGGUCGGAACAAUCAGCUUCGACGGCAAAUGGGGCAGUUGGGUAUUCCGAUUGCAGGUGAUUGGAGGCAGGGUAGUUCUUUCCGCCAUCUGGACUGUCUGGCCCUACAGGGAACGCGAUUCGUAUUCCGUCAUCCUGAAACGGGAGAUACGCAGGAAUAUAAGUUGCCUUUGCCCAAACUCUUUCGCCGCCUGAUACAUCUCGAAGAAGGCACUGCAGCAGACCAAACAAUAUCAAAGACAAAAAAACGUUCCAAAUAGGUAGCUGACUAAUUUUAAUAAUCCAACAUAUAAUA .................(((((.....(((....((((((.(((.(((.((((..((..((((......))))..))...))))....(((((......((((((...(((((((.(((((((...((((...........))))....))))..))).))))))).))))))..))).))..))).)))))))))....(.((((((((...((((.....))))......)).)))))))...)))..((((((((....((((.((((((.....))))))((((((((((.((((((((.....))))))))...((((.((..((((((((..((((((....)))))).(((......)))......)))))).)).(((....))))).))))...))).))))))).(((((....)))))...))))....((((((((......((..(((.((....((((((((((...........)))).)))))))).)))..))......))))..))))...(((((((.((.(((((.(((((((..((((((((........(((((((((((............))))))))))).............(((((((...((((...(((.((((((...((((.(((......))).)))).((((.((((.((((((((((..(((((.(((((.....)))))))))).))))))))))...))))))))......)))))).))).(((.....((((((((.((...((((((((....))))))))(((.(((........))))))..)).)))))))).))).....))))..((((((..(((((((((.(((((...)))))))).....))))))..)))))).)))))))...)))))))).))))......))).)))))...)).(((...)))...........................)))))))...))))))))...................))))).... (-352.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1035 120 71 211 162 NC_002950:PG1704|5end_thiol:disulfide_interchange_protein_dsbD,_putative_1792819..1793049_NEGATIVE 480 810 764 179 129 NC_002950:PG1116|5end_methylenetetrahydrofolate_dehydrogenase/methenyltetrahydrofolate_cyclohydrolase_1188931..1189161_POSITIVE 457 846 801 203 138 NC_002950:PG0359|5end_flavin_reductase_domain_protein_392851..393081_POSITIVE 452 810 761 77 27 NC_002950:PG0802|5end_alpha_keto_acid_dehydrogenase_complex,_E3_component,_lipoamide_dehydrogenase_859722..859952_NEGATIVE 451 829 783 199 160 NC_002950:PG1057|5end_hypothetical_protein_1123419..1123649_POSITIVE 442 799 755 213 173 NC_002950:PG1959|5end_ribosomal_protein_L33_2048405..2048635_NEGATIVE 441 809 761 70 15 NC_002950:PG0674|5end_indolepyruvate_ferredoxin_oxidoreductase,_beta_subunit_724802..725032_NEGATIVE 440 810 761 179 122 NC_002950:PG1248|5end_hypothetical_protein_1326532..1326762_NEGATIVE 438 459 411 103 53 NC_002950:PG1388|5end_hypothetical_protein_1470889..1471119_NEGATIVE 437 1008 961 200 125 NC_002950:PG0032|5end_beta-mannosidase,_putative_40212..40442_NEGATIVE 434 449 402 137 92 NC_002950:PG0268|5end_tRNA_(5-methylaminomethyl-2-thiouridylate)-methyltransferase_300898..301128_POSITIVE 433 809 761 229 169 NC_002950:PG2027|5end_hypothetical_protein_2125494..2125724_POSITIVE 431 830 784 207 149 NC_002950:PG1226|5end_peptidyl-prolyl_cis-trans_isomerase,_cyclophilin-type_1303264..1303494_POSITIVE 429 808 761 66 19 NC_002950:PG0996|5end_conserved_hypothetical_protein_TIGR01777_1055592..1055822_POSITIVE 420 778 734 60 18 NC_002950:PG1318|5end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_1397768..1397998_NEGATIVE 418 847 801 202 136 NC_002950:PG0141|5end_spoOJ_protein_164747..164977_NEGATIVE 415 799 755 65 13 NC_002950:PG1954|5end_NAD_dependent_epimerase/reductase-related_protein_2044513..2044743_POSITIVE 411 719 675 82 30 NC_002950:PG0677|5end_saccharopine_dehydrogenase_727653..727883_POSITIVE 410 229 183 56 11 NC_002950:PG0516|5end_hypothetical_protein_559184..559414_NEGATIVE 410 992 951 186 138 NC_002950:PG0158|5end_competence_protein_F-related_protein_180175..180405_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 480 810 764 55 5 NC_002950:PG1115|3end_signal_recognition_particle_protein_1188887..1189037_POSITIVE 452 810 761 72 22 NC_002950:PG0803|3end_glucosamine-6-phosphate_deaminase_859727..859877_NEGATIVE 451 760 711 125 89 NC_002950:PG1715|3end_hypothetical_protein_1803393..1803543_NEGATIVE 438 459 411 122 72 NC_002950:PG1389|3end_DNA-binding_protein,_histone-like_family_1470870..1471020_NEGATIVE 434 449 402 132 87 NC_002950:PG0267|3end_arginyl-tRNA_synthetase_300973..301123_POSITIVE 423 460 411 151 89 NC_002950:PG0973|3end_alpha-1,2-mannosidase_family_protein_1036562..1036712_POSITIVE 418 779 732 65 3 NC_002950:PG0132|3end_hypothetical_protein_153054..153204_POSITIVE 417 110 72 78 39 NC_002950:PG0332|3end_transcription_termination_factor_Rho_367675..367825_POSITIVE 415 799 755 61 9 NC_002950:PG1953|3end_YitT_family_protein_2044589..2044739_POSITIVE 409 435 391 84 43 NC_002950:PG1587|3end_PAP2_superfamily_protein_1665779..1665929_POSITIVE 409 300 258 54 5 NC_002950:PG0334|3end_glycosyl_transferase,_group_2_family_protein_368792..368942_NEGATIVE 406 837 792 41 1 NC_002950:PG0958|3end_ribonuclease_BN,_putative_1018135..1018285_NEGATIVE 406 810 763 122 38 NC_002950:PG0443|3end_hemagglutinin-related_protein_483147..483297_POSITIVE 404 1008 962 134 75 NC_002950:PG0962|3end_prolyl-tRNA_synthetase_1022327..1022477_NEGATIVE 400 810 762 145 97 NC_002950:PG2142|3end_GTP-binding_protein_Era_2248564..2248714_POSITIVE 400 777 731 82 32 NC_002950:PG1254|3end_acetyltransferase,_GNAT_family_1331594..1331744_NEGATIVE 399 810 770 148 116 NC_002950:PG1608|3end_methylmalonyl-CoA_decarboxylase,_beta_subunit_1688685..1688835_NEGATIVE 398 840 791 138 80 NC_002950:PG0344|3end_purple_acid_phosphatase_378543..378693_NEGATIVE 398 840 791 76 25 NC_002950:PG0086|3end_ATP-dependent_RNA_helicase,_DEAD/DEAH_box_family_100545..100695_NEGATIVE 397 599 554 144 101 NC_002950:PG0445|3end_peptidase_T_486549..486699_POSITIVE