Origin IGS: tgctctctacttttttattgtgttgactcttctaccattgtccgaagatcttaatgcaaagcattttcgcaaatgtagaaattattcttgattgactgattaagtattgaccatataatacgaggaaatacacttgatataatcgcatatcacggcatgctccgaaaacagaaaggtcgtgctcgggatttgttcccttgcacgacctcttaaaaaaatcaggaggagcga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tcgctcctcctgatttttttaagaggtcgtgcaagggaacaaatcccgagcacgacctttctgttttcggagcatgccgtgatatgcgattatatcaagtgtatttcctcgtattatatggtcaatacttaatcagtcaatcaagaataatttctacatttgcgaaaatgctttgcattaagatcttcggacaatggtagaagagtcaacacaataaaaaagtagagagca Mask Tandem Repeat Region ================================================ tgctctctacttttttattgtgttgactcttctaccattgtccgaagatcttaatgcaaagcattttcgcaaatgtagaaattattcttgattgactgattaagtattgaccatataatacgaggaaatacacttgatataatcgcatatcacggcatgctccgaaaacagaaaggtcgtgctcgggatttgttcccttgcacgacctcttaaaaaaatcaggaggagcga Find is-nt database================================================ Query_seq: PG1613:PG1614|PG1613:PG1614:glyoxalase family protein:succinate dehydrogenase:<-<-:1693659..1693889 231 tgctctctacttttttattgtgttgactcttctaccattgtccgaagatcttaatgcaaagcattttcgcaaatgtagaaattattcttgattgactgattaagtattgaccatataatacgaggaaatacacttgatataatcgcatatcacggcatgctccgaaaacagaaaggtcgtgctcgggatttgttcccttgcacgacctcttaaaaaaatcaggaggagcga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1613:PG1614|PG1613:PG1614:glyoxalase family protein:succinate dehydrogenase:<-<-:1693659..1693889 231 tgctctctacttttttattgtgttgactcttctaccattgtccgaagatcttaatgcaaagcattttcgcaaatgtagaaattattcttgattgactgattaagtattgaccatataatacgaggaaatacacttgatataatcgcatatcacggcatgctccgaaaacagaaaggtcgtgctcgggatttgttcccttgcacgacctcttaaaaaaatcaggaggagcga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1613:PG1614|PG1613:PG1614:glyoxalase family protein:succinate dehydrogenase:<-<-:1693659..1693889 231 tgctctctacttttttattgtgttgactcttctaccattgtccgaagatcttaatgcaaagcattttcgcaaatgtagaaattattcttgattgactgattaagtattgaccatataatacgaggaaatacacttgatataatcgcatatcacggcatgctccgaaaacagaaaggtcgtgctcgggatttgttcccttgcacgacctcttaaaaaaatcaggaggagcga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgctctctacttttttattgtgttgactcttctaccattgtccgaagatcttaatgcaaagcattttcgcaaatgtagaaattattcttgattgactgattaagtattgaccatataatacgaggaaatacacttgatataatcgcatatcacggcatgctccgaaaacagaaaggtcgtgctcgggatttgttcccttgcacgacctcttaaaaaaatcaggaggagcga Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 106 End: 210 ......................................................................................................... M D H I I R G N T L D I I A Y H G M L R K Q K G R A R D L F P C T T S ..................... Protein_Len: 40 Strand: - Start: 103 End: 222 ...................................................................................................... T N V M Y Y S S I C K I Y D C I V A H E S F L F T T S P I Q E R A R G R L F I M ......... Protein_Len: 30 Strand: - Start: 54 End: 143 ..................................................... H L A N E C I Y F N N K I S Q N L I S W I I R P F V S S I M ........................................................................................ Protein_Len: 38 Strand: - Start: 35 End: 148 .................................. W Q G F I K I C L M K A F T S I I R S Q S I L Y Q G Y L V L F Y V Q Y L R M ................................................................................... tgctctctacttttttattgtgttgactcttctaccattgtccgaagatcttaatgcaaagcattttcgcaaatgtagaaattattcttgattgactgattaagtattgaccatataatacgaggaaatacacttgatataatcgcatatcacggcatgctccgaaaacagaaaggtcgtgctcgggatttgttcccttgcacgacctcttaaaaaaatcaggaggagcga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgctctctacttttttattgtgttgactcttctaccattgtccgaagatcttaatgcaaagcattttcgcaaatgtagaaattattcttgattgactgattaagtattgaccatataatacgaggaaatacacttgatataatcgcatatcacggcatgctccgaaaacagaaaggtcgtgctcgggatttgttcccttgcacgacctcttaaaaaaatcaggaggagcga Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 75 HP_score: -7.5 Tail_Score: -4.08767 Start: 169 End: 212 Full_Region: tcctcctgatttttt taagaggtcgtgcaaggga acaaa tcccgagcacgacctttctg ttttcggagcatgcc ........................................................................................................................................................................taagaggtcgtgcaagggaacaaatcccgagcacgacctttctg................... TransTerm Strand: - Conf: 81 HP_score: -10.4 Tail_Score: -3.6422 Start: 171 End: 211 Full_Region: cctcctgattttttt aagaggtcgtgcaaggga acaaa tcccgagcacgacctttc tgttttcggagcatg ..........................................................................................................................................................................aagaggtcgtgcaagggaacaaatcccgagcacgacctttc.................... TransTerm Strand: + Conf: 95 HP_score: -13.4 Tail_Score: -3.02511 Start: 175 End: 207 Full_Region: CTCCGAAAACAGAAA GGTCGTGCTCGGGA TTTGT TCCCTTGCACGACC TCTTAAAAAAATCAG ..............................................................................................................................................................................GGTCGTGCTCGGGATTTGTTCCCTTGCACGACC........................ Find igs database================================================ Query_seq: PG1613:PG1614|PG1613:PG1614:glyoxalase family protein:succinate dehydrogenase:<-<-:1693659..1693889 231 tgctctctacttttttattgtgttgactcttctaccattgtccgaagatcttaatgcaaagcattttcgcaaatgtagaaattattcttgattgactgattaagtattgaccatataatacgaggaaatacacttgatataatcgcatatcacggcatgctccgaaaacagaaaggtcgtgctcgggatttgttcccttgcacgacctcttaaaaaaatcaggaggagcga Intra-Species Hit: Count: 1 Min: 1 Max: 211 Len: 211 Subject: NC_002950_PG1613_PG1614|glyoxalase family protein:succinate dehydrogenase|NEGATIVE:NEGATIVE|[1693659,1693889]|231 HSP 1 e-value: 1.0E-116 bit: 418.0 Len: 211 Query Start:1 Query End:211 Subject Strand: POSITIVE Subject Start: 1 Subject End: 211 tgctctctacttttttattgtgttgactcttctaccattgtccgaagatcttaatgcaaagcattttcgcaaatgtagaaattattcttgattgactgattaagtattgaccatataatacgaggaaatacacttgatataatcgcatatcacggcatgctccgaaaacagaaaggtcgtgctcgggatttgttcccttgcacgacctctt.................... tgctctctacttttttattgtgttgactcttctaccattgtccgaagatcttaatgcaaagcattttcgcaaatgtagaaattattcttgattgactgattaagtattgaccatataatacgaggaaatacacttgatataatcgcatatcacggcatgctccgaaaacagaaaggtcgtgctcgggatttgttcccttgcacgacctctt.................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGCUCUCUACUUUUUUAUUGUGUUGACUCUUCUACCAUUGUCCGAAGAUCUUAAUGCAAAGCAUUUUCGCAAAUGUAGAAAUUAUUCUUGAUUGACUGAUUAAGUAUUGACCAUAUAAUACGAGGAAAUACACUUGAUAUAAUCGCAUAUCACGGCAUGCUCCGAAAACAGAAAGGUCGUGCUCGGGAUUUGUUCCCUUGCACGACCUCUU ((((..............((((((..(((.(((((.(((...((((((((((......))).)))))))..))))))))..(((..(((((((....)))))))...)))...........)))..))))))..((((((......)))))).))))................(((((((((..((((.....))))..)))))))))... (-55.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGAGGUCGUGCAAGGGAACAAAUCCCGAGCACGACCUUUCUGUUUUCGGAGCAUGCCGUGAUAUGCGAUUAUAUCAAGUGUAUUUCCUCGUAUUAUAUGGUCAAUACUUAAUCAGUCAAUCAAGAAUAAUUUCUACAUUUGCGAAAAUGCUUUGCAUUAAGAUCUUCGGACAAUGGUAGAAGAGUCAACACAAUAAAAAAGUAGAGAGCA .(((((((((((..((((.....))))..)))))))))))(((.....((((.(((((.(((((((....))))))).).))))))))..(((((........))))).....)))..............(((((((...((((((.....))))))....(((((((..((....)).))))).))..............)))))))... (-58.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 872 70 21 161 112 NC_002950:PG1613|5end_glyoxalase_family_protein_1693568..1693798_NEGATIVE 369 210 163 40 1 NC_002950:PG1249|5end_1-acyl-sn-glycerol-3-phosphate_acetyltransferase,_putative_1327161..1327391_NEGATIVE 361 210 169 190 156 NC_002950:PG0387|5end_elongation_factor_Tu_420643..420873_POSITIVE 359 199 151 174 123 NC_002950:PG1937|5end_50S_ribosomal_protein_L4_2024136..2024366_NEGATIVE 346 46 1 85 28 NC_002950:PG1983|5end_CRISPR-associated_TM1791_family_protein_2073442..2073672_NEGATIVE 339 199 153 192 137 NC_002950:PG1594|5end_ComEC/Rec2-related_protein_1673587..1673817_NEGATIVE 337 200 154 60 8 NC_002950:PG0309|5end_thiamine_biosynthesis_lipoprotein_ApbE_345023..345253_NEGATIVE 332 200 153 57 2 NC_002950:PG2109|5end_thiamine-phosphate_pyrophosphorylase_2219452..2219682_NEGATIVE 330 197 152 143 82 NC_002950:PG0470|5end_hypothetical_protein_511883..512113_NEGATIVE 325 186 144 51 13 NC_002950:PG1305|5end_glycine_dehydrogenase_1386538..1386768_NEGATIVE 322 190 142 179 139 NC_002950:PG1729|5end_thiol_peroxidase_1818881..1819111_NEGATIVE 319 200 151 96 32 NC_002950:PG0608|5end_hypothetical_protein_662945..663175_POSITIVE 318 187 145 200 136 NC_002950:PG2187|5end_1,4-dihydroxy-2-naphthoate_octaprenyltransferase_2294643..2294873_NEGATIVE 316 185 143 213 167 NC_002950:PG1285|5end_glucosamine-6-phosphate_deaminase_1362894..1363124_POSITIVE 316 193 151 73 26 NC_002950:PG0223|5end_exonuclease_257610..257840_NEGATIVE 314 190 144 73 25 NC_002950:PG0850|5end_DNA_binding_protein,_excisionase_family,_putative_911578..911808_NEGATIVE 311 198 153 206 144 NC_002950:PG0451|5end_CBS_domain_protein_491519..491749_POSITIVE 309 188 142 222 181 NC_002950:PG1102|5end_hypothetical_protein_1173637..1173867_NEGATIVE 307 198 151 225 177 NC_002950:PG1702|5end_DNA_gyrase,_B_subunit_1789910..1790140_NEGATIVE 307 163 121 89 55 NC_002950:PG1638|5end_thioredoxin_family_protein_1719650..1719880_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 346 46 1 88 31 NC_002950:PG1984|3end_hypothetical_protein_2073439..2073589_NEGATIVE 337 200 154 63 11 NC_002950:PG0310|3end_nitroreductase_family_protein_345020..345170_NEGATIVE 332 200 153 60 5 NC_002950:PG2110|3end_thiamine_biosynthesis_protein_ThiC_2219449..2219599_NEGATIVE 325 186 144 46 8 NC_002950:PG1306|3end_metallo-beta-lactamase_family_protein_1386543..1386693_NEGATIVE 322 190 142 133 93 NC_002950:PG1730|3end_O-methyltransferase_family_protein_1818927..1819077_NEGATIVE 319 200 151 65 1 NC_002950:PG0607|3end_hypothetical_protein_662994..663144_POSITIVE 316 188 142 53 1 NC_002950:PG1416|3end_enoyl-(acyl-carrier-protein)_reductase_II_1501078..1501228_POSITIVE 316 193 151 69 22 NC_002950:PG0224|3end_hypothetical_protein_257614..257764_NEGATIVE 314 190 144 85 37 NC_002950:PG0851|3end_hypothetical_protein_911566..911716_NEGATIVE 308 196 153 142 78 NC_002950:PG0085|3end_alpha-galactosidase_100556..100706_POSITIVE 307 210 171 79 34 NC_002950:PG0561|3end_peptidase,_M20/M25/M40_family_617700..617850_NEGATIVE 305 210 161 140 72 NC_002950:PG2185|3end_transporter,_putative_2291933..2292083_NEGATIVE 305 181 143 120 75 NC_002950:PG0890|3end_alkaline_phosphatase,_putative_953103..953253_POSITIVE 303 189 142 68 1 NC_002950:PG1052|3end_transcriptional_regulator,_putative_1120126..1120276_POSITIVE 301 188 145 56 9 NC_002950:PG0378|3end_elongation_factor_Ts_408808..408958_POSITIVE 300 209 166 68 20 NC_002950:PG1741|3end_aspartate_ammonia-lyase_1825856..1826006_NEGATIVE 297 200 152 64 10 NC_002950:PG1953|3end_YitT_family_protein_2044589..2044739_POSITIVE 297 186 141 134 84 NC_002950:PG0746|3end_sensor_histidine_kinase_792536..792686_NEGATIVE 294 209 164 77 23 NC_002950:PG0758|3end_peptidyl-dipeptidase_Dcp_806467..806617_NEGATIVE 290 190 153 41 1 NC_002950:PG0441|3end_hypothetical_protein_481447..481597_POSITIVE