Origin IGS: ttgcaaatgggctttcacagacaaactttagtctattttttgatcagattcaatgatttaagcaaaagaaattggctatcaacagctcgcacttttcaatagcaagtgtaataacggaacaaatttaaggcaaaaactgagaaatgagttctgagcttcgtcttttcgaacaggctgttcgataccaatagcgataacaataaaagcgcagcaaccgggcctatacggctgggatttttgctacttttgtagagcgatagggagttctcgacaatgtaaatggtggttctggaagggcttgtctgtcgaatagaacagctttacgaacgtttactaactacttaatagcaacagatatgatgcactctgacttctctctttctatacggtcattaggtggatttcatgcggcctatacaggtatgtaccatcgcgtggcatgtggacaaaggagatattattccatgaggagtaagcacgaataggcattcgatagcttctataacgtataaggcacccacccgattctttcataaatcagatgggtgcctctgttttatcagcttacaatatggcattttgggggctgcgattgatatacaaatcattttcgatttctatatatattgaaaacgatttatatataaatggaaaatggtttatatatagatcgttattcgatataacgagaaaggtatttcaatctgaaaaaggagtatttccatctcttttgtatcataaccagacttcttagagccggcttatttggaatgattctaaactcctcttcgctgttgaaaacttttttggcacctatttttcggtcacaataccgattttcttagttgtttttaggcctaagatccttctctcttcagcctgcagcggtgcaggctgaggcgttttttcacttctgtcgatgccctcttcgagcaaggattggagaaatagctgtcgtatcattagcgcaacttctcctcgccggatgcaaaagaaaaaccgcccaccgaacagaaaagtctgttcaatgggcggcttattgagattatggaaactttc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gaaagtttccataatctcaataagccgcccattgaacagacttttctgttcggtgggcggtttttcttttgcatccggcgaggagaagttgcgctaatgatacgacagctatttctccaatccttgctcgaagagggcatcgacagaagtgaaaaaacgcctcagcctgcaccgctgcaggctgaagagagaaggatcttaggcctaaaaacaactaagaaaatcggtattgtgaccgaaaaataggtgccaaaaaagttttcaacagcgaagaggagtttagaatcattccaaataagccggctctaagaagtctggttatgatacaaaagagatggaaatactcctttttcagattgaaatacctttctcgttatatcgaataacgatctatatataaaccattttccatttatatataaatcgttttcaatatatatagaaatcgaaaatgatttgtatatcaatcgcagcccccaaaatgccatattgtaagctgataaaacagaggcacccatctgatttatgaaagaatcgggtgggtgccttatacgttatagaagctatcgaatgcctattcgtgcttactcctcatggaataatatctcctttgtccacatgccacgcgatggtacatacctgtataggccgcatgaaatccacctaatgaccgtatagaaagagagaagtcagagtgcatcatatctgttgctattaagtagttagtaaacgttcgtaaagctgttctattcgacagacaagcccttccagaaccaccatttacattgtcgagaactccctatcgctctacaaaagtagcaaaaatcccagccgtataggcccggttgctgcgcttttattgttatcgctattggtatcgaacagcctgttcgaaaagacgaagctcagaactcatttctcagtttttgccttaaatttgttccgttattacacttgctattgaaaagtgcgagctgttgatagccaatttcttttgcttaaatcattgaatctgatcaaaaaatagactaaagtttgtctgtgaaagcccatttgcaa Mask Tandem Repeat Region ================================================ ttgcaaatgggctttcacagacaaactttagtctattttttgatcagattcaatgatttaagcaaaagaaattggctatcaacagctcgcacttttcaatagcaagtgtaataacggaacaaatttaaggcaaaaactgagaaatgagttctgagcttcgtcttttcgaacaggctgttcgataccaatagcgataacaataaaagcgcagcaaccgggcctatacggctgggatttttgctacttttgtagagcgatagggagttctcgacaatgtaaatggtggttctggaagggcttgtctgtcgaatagaacagctttacgaacgtttactaactacttaatagcaacagatatgatgcactctgacttctctctttctatacggtcattaggtggatttcatgcggcctatacaggtatgtaccatcgcgtggcatgtggacaaaggagatattattccatgaggagtaagcacgaataggcattcgatagcttctataacgtataaggcacccacccgattctttcataaatcagatgggtgcctctgttttatcagcttacaatatggcattttgggggctgcgattgatatacaaatcattttnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngatcgttattcgatataacgagaaaggtatttcaatctgaaaaaggagtatttccatctcttttgtatcataaccagacttcttagagccggcttatttggaatgattctaaactcctcttcgctgttgaaaacttttttggcacctatttttcggtcacaataccgattttcttagttgtttttaggcctaagatccttctctcttcagcctgcagcggtgcaggctgaggcgttttttcacttctgtcgatgccctcttcgagcaaggattggagaaatagctgtcgtatcattagcgcaacttctcctcgccggatgcaaaagaaaaaccgcccaccgaacagaaaagtctgttcaatgggcggcttattgagattatggaaactttc Find is-nt database================================================ Query_seq: PG1606:PG1608|PG1606:PG1608:ion transporter:methylmalonyl-CoA decarboxylase, beta subunit:<-<-:1687716..1688774 1059 ttgcaaatgggctttcacagacaaactttagtctattttttgatcagattcaatgatttaagcaaaagaaattggctatcaacagctcgcacttttcaatagcaagtgtaataacggaacaaatttaaggcaaaaactgagaaatgagttctgagcttcgtcttttcgaacaggctgttcgataccaatagcgataacaataaaagcgcagcaaccgggcctatacggctgggatttttgctacttttgtagagcgatagggagttctcgacaatgtaaatggtggttctggaagggcttgtctgtcgaatagaacagctttacgaacgtttactaactacttaatagcaacagatatgatgcactctgacttctctctttctatacggtcattaggtggatttcatgcggcctatacaggtatgtaccatcgcgtggcatgtggacaaaggagatattattccatgaggagtaagcacgaataggcattcgatagcttctataacgtataaggcacccacccgattctttcataaatcagatgggtgcctctgttttatcagcttacaatatggcattttgggggctgcgattgatatacaaatcattttcgatttctatatatattgaaaacgatttatatataaatggaaaatggtttatatatagatcgttattcgatataacgagaaaggtatttcaatctgaaaaaggagtatttccatctcttttgtatcataaccagacttcttagagccggcttatttggaatgattctaaactcctcttcgctgttgaaaacttttttggcacctatttttcggtcacaataccgattttcttagttgtttttaggcctaagatccttctctcttcagcctgcagcggtgcaggctgaggcgttttttcacttctgtcgatgccctcttcgagcaaggattggagaaatagctgtcgtatcattagcgcaacttctcctcgccggatgcaaaagaaaaaccgcccaccgaacagaaaagtctgttcaatgggcggcttattgagattatggaaactttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1606:PG1608|PG1606:PG1608:ion transporter:methylmalonyl-CoA decarboxylase, beta subunit:<-<-:1687716..1688774 1059 ttgcaaatgggctttcacagacaaactttagtctattttttgatcagattcaatgatttaagcaaaagaaattggctatcaacagctcgcacttttcaatagcaagtgtaataacggaacaaatttaaggcaaaaactgagaaatgagttctgagcttcgtcttttcgaacaggctgttcgataccaatagcgataacaataaaagcgcagcaaccgggcctatacggctgggatttttgctacttttgtagagcgatagggagttctcgacaatgtaaatggtggttctggaagggcttgtctgtcgaatagaacagctttacgaacgtttactaactacttaatagcaacagatatgatgcactctgacttctctctttctatacggtcattaggtggatttcatgcggcctatacaggtatgtaccatcgcgtggcatgtggacaaaggagatattattccatgaggagtaagcacgaataggcattcgatagcttctataacgtataaggcacccacccgattctttcataaatcagatgggtgcctctgttttatcagcttacaatatggcattttgggggctgcgattgatatacaaatcattttcgatttctatatatattgaaaacgatttatatataaatggaaaatggtttatatatagatcgttattcgatataacgagaaaggtatttcaatctgaaaaaggagtatttccatctcttttgtatcataaccagacttcttagagccggcttatttggaatgattctaaactcctcttcgctgttgaaaacttttttggcacctatttttcggtcacaataccgattttcttagttgtttttaggcctaagatccttctctcttcagcctgcagcggtgcaggctgaggcgttttttcacttctgtcgatgccctcttcgagcaaggattggagaaatagctgtcgtatcattagcgcaacttctcctcgccggatgcaaaagaaaaaccgcccaccgaacagaaaagtctgttcaatgggcggcttattgagattatggaaactttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1606:PG1608|PG1606:PG1608:ion transporter:methylmalonyl-CoA decarboxylase, beta subunit:<-<-:1687716..1688774 1059 ttgcaaatgggctttcacagacaaactttagtctattttttgatcagattcaatgatttaagcaaaagaaattggctatcaacagctcgcacttttcaatagcaagtgtaataacggaacaaatttaaggcaaaaactgagaaatgagttctgagcttcgtcttttcgaacaggctgttcgataccaatagcgataacaataaaagcgcagcaaccgggcctatacggctgggatttttgctacttttgtagagcgatagggagttctcgacaatgtaaatggtggttctggaagggcttgtctgtcgaatagaacagctttacgaacgtttactaactacttaatagcaacagatatgatgcactctgacttctctctttctatacggtcattaggtggatttcatgcggcctatacaggtatgtaccatcgcgtggcatgtggacaaaggagatattattccatgaggagtaagcacgaataggcattcgatagcttctataacgtataaggcacccacccgattctttcataaatcagatgggtgcctctgttttatcagcttacaatatggcattttgggggctgcgattgatatacaaatcattttcgatttctatatatattgaaaacgatttatatataaatggaaaatggtttatatatagatcgttattcgatataacgagaaaggtatttcaatctgaaaaaggagtatttccatctcttttgtatcataaccagacttcttagagccggcttatttggaatgattctaaactcctcttcgctgttgaaaacttttttggcacctatttttcggtcacaataccgattttcttagttgtttttaggcctaagatccttctctcttcagcctgcagcggtgcaggctgaggcgttttttcacttctgtcgatgccctcttcgagcaaggattggagaaatagctgtcgtatcattagcgcaacttctcctcgccggatgcaaaagaaaaaccgcccaccgaacagaaaagtctgttcaatgggcggcttattgagattatggaaactttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttgcaaatgggctttcacagacaaactttagtctattttttgatcagattcaatgatttaagcaaaagaaattggctatcaacagctcgcacttttcaatagcaagtgtaataacggaacaaatttaaggcaaaaactgagaaatgagttctgagcttcgtcttttcgaacaggctgttcgataccaatagcgataacaataaaagcgcagcaaccgggcctatacggctgggatttttgctacttttgtagagcgatagggagttctcgacaatgtaaatggtggttctggaagggcttgtctgtcgaatagaacagctttacgaacgtttactaactacttaatagcaacagatatgatgcactctgacttctctctttctatacggtcattaggtggatttcatgcggcctatacaggtatgtaccatcgcgtggcatgtggacaaaggagatattattccatgaggagtaagcacgaataggcattcgatagcttctataacgtataaggcacccacccgattctttcataaatcagatgggtgcctctgttttatcagcttacaatatggcattttgggggctgcgattgatatacaaatcattttcgatttctatatatattgaaaacgatttatatataaatggaaaatggtttatatatagatcgttattcgatataacgagaaaggtatttcaatctgaaaaaggagtatttccatctcttttgtatcataaccagacttcttagagccggcttatttggaatgattctaaactcctcttcgctgttgaaaacttttttggcacctatttttcggtcacaataccgattttcttagttgtttttaggcctaagatccttctctcttcagcctgcagcggtgcaggctgaggcgttttttcacttctgtcgatgccctcttcgagcaaggattggagaaatagctgtcgtatcattagcgcaacttctcctcgccggatgcaaaagaaaaaccgcccaccgaacagaaaagtctgttcaatgggcggcttattgagattatggaaactttc Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 1 End: 99 M Q M G F H R Q T L V Y F L I R F N D L S K R N W L S T A R T F Q ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 33 Strand: + Start: 151 End: 249 ...................................................................................................................................................... M S F V F S N R L F D T N S D N N K S A A T G P I R L G F L L L L .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 43 Strand: + Start: 182 End: 310 ..................................................................................................................................................................................... M P I A I T I K A Q Q P G L Y G W D F C Y F C R A I G S S R Q C K W W F W K G L S V E ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 303 End: 482 .............................................................................................................................................................................................................................................................................................................. M S N R T A L R T F T N Y L I A T D M M H S D F S L S I R S L G G F H A A Y T G M Y H R V A C G Q R R Y Y S M R S K H E ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 45 Strand: + Start: 493 End: 627 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M A S I T Y K A P T R F F H K S D G C L C F I S L Q Y G I L G A A I D I Q I I F D F Y I Y ................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 509 End: 751 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M R H P P D S F I N Q M G A S V L S A Y N M A F W G L R L I Y K S F S I S I Y I E N D L Y I N G K W F I Y R S L F D I T .................................................................................................................................................................................................................................................................................................................... Protein_Len: 46 Strand: + Start: 705 End: 842 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M K K E Y F H L F C I I T R L L R A G L F G M I L N S S S L L K T F L A P I F R S Q Y R F S ......................................................................................................................................................................................................................... Protein_Len: 44 Strand: + Start: 764 End: 895 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M W N D S K L L F A V E N F F G T Y F S V T I P I F L V V F R P K I L L S S A C S G A G .................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 846 End: 1040 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M F L G L R S F S L Q P A A V Q A E A F F H F C R C P L R A R I G E I A V V S L A Q L L L A G C K R K T A H R T E K S V ................... Protein_Len: 48 Strand: + Start: 914 End: 1057 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M S M P S S S K D W R N S C R I I S A T S P R R M Q K K N R P P N R K V C S M G G L L R L W K L .. Protein_Len: 60 Strand: - Start: 817 End: 1029 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ K L G L I R R E E A Q L P A P Q P T K E S R D I G E E L L S Q L F L Q R I M L A V E G R R I C F F F R G G F L F T Q E M .............................. Protein_Len: 47 Strand: - Start: 677 End: 817 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E I Y R S L Y K L R F F S Y K W R K Q I M V L S R L A P K N P I I R F E E E S N F V K K A G M .................................................................................................................................................................................................................................................. Protein_Len: 39 Strand: - Start: 568 End: 684 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... L I A N Q P S R N I Y L D N E I E I Y I S F S K Y I F P F H N I Y L D N N S M ....................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 42 Strand: - Start: 396 End: 521 ........................................................................................................................................................................................................................................................................................................................................................................................................... T S K M R G I C T H V M A H C T S L P S I I G H P T L V F L C E I A E I V Y L A G M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 50 Strand: - Start: 385 End: 534 ................................................................................................................................................................................................................................................................................................................................................................................................ V T M L H I E H P R Y L Y T G D R P M H V F S I N N W S S Y A R I P M R Y S R Y R I L C G G S E K M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 99 End: 311 .................................................................................................. F V S F H T R L K T K E F L S N S V L L S L L L L A A V P G I R S P N K S S K Y L S L S N E V I Y I T T R S P S T Q R M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 40 Strand: - Start: 82 End: 201 ................................................................................. C S A S K L L L H L L P V F K L C F S L F S N Q A E D K R V P Q E I G I A I V M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ttgcaaatgggctttcacagacaaactttagtctattttttgatcagattcaatgatttaagcaaaagaaattggctatcaacagctcgcacttttcaatagcaagtgtaataacggaacaaatttaaggcaaaaactgagaaatgagttctgagcttcgtcttttcgaacaggctgttcgataccaatagcgataacaataaaagcgcagcaaccgggcctatacggctgggatttttgctacttttgtagagcgatagggagttctcgacaatgtaaatggtggttctggaagggcttgtctgtcgaatagaacagctttacgaacgtttactaactacttaatagcaacagatatgatgcactctgacttctctctttctatacggtcattaggtggatttcatgcggcctatacaggtatgtaccatcgcgtggcatgtggacaaaggagatattattccatgaggagtaagcacgaataggcattcgatagcttctataacgtataaggcacccacccgattctttcataaatcagatgggtgcctctgttttatcagcttacaatatggcattttgggggctgcgattgatatacaaatcattttcgatttctatatatattgaaaacgatttatatataaatggaaaatggtttatatatagatcgttattcgatataacgagaaaggtatttcaatctgaaaaaggagtatttccatctcttttgtatcataaccagacttcttagagccggcttatttggaatgattctaaactcctcttcgctgttgaaaacttttttggcacctatttttcggtcacaataccgattttcttagttgtttttaggcctaagatccttctctcttcagcctgcagcggtgcaggctgaggcgttttttcacttctgtcgatgccctcttcgagcaaggattggagaaatagctgtcgtatcattagcgcaacttctcctcgccggatgcaaaagaaaaaccgcccaccgaacagaaaagtctgttcaatgggcggcttattgagattatggaaactttc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 658 End: 687 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTTTATATATAGATCGTTATTCGATATAAC.................................................................................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttgcaaatgggctttcacagacaaactttagtctattttttgatcagattcaatgatttaagcaaaagaaattggctatcaacagctcgcacttttcaatagcaagtgtaataacggaacaaatttaaggcaaaaactgagaaatgagttctgagcttcgtcttttcgaacaggctgttcgataccaatagcgataacaataaaagcgcagcaaccgggcctatacggctgggatttttgctacttttgtagagcgatagggagttctcgacaatgtaaatggtggttctggaagggcttgtctgtcgaatagaacagctttacgaacgtttactaactacttaatagcaacagatatgatgcactctgacttctctctttctatacggtcattaggtggatttcatgcggcctatacaggtatgtaccatcgcgtggcatgtggacaaaggagatattattccatgaggagtaagcacgaataggcattcgatagcttctataacgtataaggcacccacccgattctttcataaatcagatgggtgcctctgttttatcagcttacaatatggcattttgggggctgcgattgatatacaaatcattttcgatttctatatatattgaaaacgatttatatataaatggaaaatggtttatatatagatcgttattcgatataacgagaaaggtatttcaatctgaaaaaggagtatttccatctcttttgtatcataaccagacttcttagagccggcttatttggaatgattctaaactcctcttcgctgttgaaaacttttttggcacctatttttcggtcacaataccgattttcttagttgtttttaggcctaagatccttctctcttcagcctgcagcggtgcaggctgaggcgttttttcacttctgtcgatgccctcttcgagcaaggattggagaaatagctgtcgtatcattagcgcaacttctcctcgccggatgcaaaagaaaaaccgcccaccgaacagaaaagtctgttcaatgggcggcttattgagattatggaaactttc Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 70 HP_score: -3.4 Tail_Score: -4.95547 Start: 206 End: 220 Full_Region: atcccagccgtatag gcccgg ttg ctgcgc ttttattgttatcgc .............................................................................................................................................................................................................gcccggttgctgcgc....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 66 HP_score: -4.4 Tail_Score: -4.42788 Start: 510 End: 554 Full_Region: AGCTTCTATAACGTA TAAGGCACCCACCCGATT CTTTCATA AATCAGATGGGTGCCTCTG TTTTATCAGCTTACA .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TAAGGCACCCACCCGATTCTTTCATAAATCAGATGGGTGCCTCTG......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 71 HP_score: -7.3 Tail_Score: -3.81948 Start: 511 End: 552 Full_Region: GCTTCTATAACGTAT AAGGCACCCACCCGATT CTTTCATA AATCAGATGGGTGCCTC TGTTTTATCAGCTTA ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................AAGGCACCCACCCGATTCTTTCATAAATCAGATGGGTGCCTC........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -15.0 Tail_Score: -3.45054 Start: 513 End: 550 Full_Region: agctgataaaacaga ggcacccatctgattt atgaaa gaatcgggtgggtgcc ttatacgttatagaa ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggcacccatctgatttatgaaagaatcgggtgggtgcc............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 77 HP_score: -9.9 Tail_Score: -3.48517 Start: 513 End: 550 Full_Region: TTCTATAACGTATAA GGCACCCACCCGATT CTTTCATA AATCAGATGGGTGCC TCTGTTTTATCAGCT ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GGCACCCACCCGATTCTTTCATAAATCAGATGGGTGCC............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 76 HP_score: -7.1 Tail_Score: -4.1399 Start: 605 End: 658 Full_Region: CGATTGATATACAAA TCATTTTCGATTTCTATATATATTG AAAA CGATTTATATATAAATGGAAAATGG TTTATATATAGATCG ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TCATTTTCGATTTCTATATATATTGAAAACGATTTATATATAAATGGAAAATGG................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 61 HP_score: -3.9 Tail_Score: -4.27259 Start: 634 End: 673 Full_Region: cgttatatcgaataa cgatctatatataaa ccattttcca tttatatataaatcg ttttcaatatatata .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cgatctatatataaaccattttccatttatatataaatcg.................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 66 HP_score: -6.8 Tail_Score: -3.70773 Start: 634 End: 673 Full_Region: TATATATATTGAAAA CGATTTATATATAAAT GGAAAATG GTTTATATATAGATCG TTATTCGATATAACG .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CGATTTATATATAAATGGAAAATGGTTTATATATAGATCG.................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 74 HP_score: -4.7 Tail_Score: -4.85618 Start: 821 End: 836 Full_Region: TTTGGCACCTATTTT TCGGT CACAAT ACCGA TTTTCTTAGTTGTTT ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TCGGTCACAATACCGA............................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -15.2 Tail_Score: -5.20154 Start: 871 End: 902 Full_Region: GCCTAAGATCCTTCT CTCTTCAGCCTGCA GCGG TGCAGGCTGAGGCG TTTTTTCACTTCTGT ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CTCTTCAGCCTGCAGCGGTGCAGGCTGAGGCG............................................................................................................................................................. TransTerm Strand: + Conf: 100 HP_score: -16.4 Tail_Score: -2.81195 Start: 873 End: 900 Full_Region: CTAAGATCCTTCTCT CTTCAGCCTGCA GCGG TGCAGGCTGAGG CGTTTTTTCACTTCT ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CTTCAGCCTGCAGCGGTGCAGGCTGAGG............................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -20.6 Tail_Score: -5.48213 Start: 1000 End: 1035 Full_Region: cataatctcaataag ccgcccattgaacaga cttt tctgttcggtgggcgg tttttcttttgcatc .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ccgcccattgaacagacttttctgttcggtgggcgg........................ Find igs database================================================ Query_seq: PG1606:PG1608|PG1606:PG1608:ion transporter:methylmalonyl-CoA decarboxylase, beta subunit:<-<-:1687716..1688774 1059 ttgcaaatgggctttcacagacaaactttagtctattttttgatcagattcaatgatttaagcaaaagaaattggctatcaacagctcgcacttttcaatagcaagtgtaataacggaacaaatttaaggcaaaaactgagaaatgagttctgagcttcgtcttttcgaacaggctgttcgataccaatagcgataacaataaaagcgcagcaaccgggcctatacggctgggatttttgctacttttgtagagcgatagggagttctcgacaatgtaaatggtggttctggaagggcttgtctgtcgaatagaacagctttacgaacgtttactaactacttaatagcaacagatatgatgcactctgacttctctctttctatacggtcattaggtggatttcatgcggcctatacaggtatgtaccatcgcgtggcatgtggacaaaggagatattattccatgaggagtaagcacgaataggcattcgatagcttctataacgtataaggcacccacccgattctttcataaatcagatgggtgcctctgttttatcagcttacaatatggcattttgggggctgcgattgatatacaaatcattttcgatttctatatatattgaaaacgatttatatataaatggaaaatggtttatatatagatcgttattcgatataacgagaaaggtatttcaatctgaaaaaggagtatttccatctcttttgtatcataaccagacttcttagagccggcttatttggaatgattctaaactcctcttcgctgttgaaaacttttttggcacctatttttcggtcacaataccgattttcttagttgtttttaggcctaagatccttctctcttcagcctgcagcggtgcaggctgaggcgttttttcacttctgtcgatgccctcttcgagcaaggattggagaaatagctgtcgtatcattagcgcaacttctcctcgccggatgcaaaagaaaaaccgcccaccgaacagaaaagtctgttcaatgggcggcttattgagattatggaaactttc Intra-Species Hit: Count: 2 Min: 1 Max: 1059 Len: 1059 Subject: NC_002950_PG1606_PG1608|ion transporter:methylmalonyl-CoA decarboxylase, beta subunit|NEGATIVE:NEGATIVE|[1687716,1688774]|1059 HSP 1 e-value: 0.0 bit: 1225.0 Len: 618 Query Start:1 Query End:618 Subject Strand: POSITIVE Subject Start: 1 Subject End: 618 ttgcaaatgggctttcacagacaaactttagtctattttttgatcagattcaatgatttaagcaaaagaaattggctatcaacagctcgcacttttcaatagcaagtgtaataacggaacaaatttaaggcaaaaactgagaaatgagttctgagcttcgtcttttcgaacaggctgttcgataccaatagcgataacaataaaagcgcagcaaccgggcctatacggctgggatttttgctacttttgtagagcgatagggagttctcgacaatgtaaatggtggttctggaagggcttgtctgtcgaatagaacagctttacgaacgtttactaactacttaatagcaacagatatgatgcactctgacttctctctttctatacggtcattaggtggatttcatgcggcctatacaggtatgtaccatcgcgtggcatgtggacaaaggagatattattccatgaggagtaagcacgaataggcattcgatagcttctataacgtataaggcacccacccgattctttcataaatcagatgggtgcctctgttttatcagcttacaatatggcattttgggggctgcgattgatatacaaatcattttcgatttc......................................................................................................................................................................................................................................................................................................................................................................................................................................................... ttgcaaatgggctttcacagacaaactttagtctattttttgatcagattcaatgatttaagcaaaagaaattggctatcaacagctcgcacttttcaatagcaagtgtaataacggaacaaatttaaggcaaaaactgagaaatgagttctgagcttcgtcttttcgaacaggctgttcgataccaatagcgataacaataaaagcgcagcaaccgggcctatacggctgggatttttgctacttttgtagagcgatagggagttctcgacaatgtaaatggtggttctggaagggcttgtctgtcgaatagaacagctttacgaacgtttactaactacttaatagcaacagatatgatgcactctgacttctctctttctatacggtcattaggtggatttcatgcggcctatacaggtatgtaccatcgcgtggcatgtggacaaaggagatattattccatgaggagtaagcacgaataggcattcgatagcttctataacgtataaggcacccacccgattctttcataaatcagatgggtgcctctgttttatcagcttacaatatggcattttgggggctgcgattgatatacaaatcattttcgatttc......................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 0.0 bit: 775.0 Len: 391 Query Start:669 Query End:1059 Subject Strand: POSITIVE Subject Start: 669 Subject End: 1059 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gatcgttattcgatataacgagaaaggtatttcaatctgaaaaaggagtatttccatctcttttgtatcataaccagacttcttagagccggcttatttggaatgattctaaactcctcttcgctgttgaaaacttttttggcacctatttttcggtcacaataccgattttcttagttgtttttaggcctaagatccttctctcttcagcctgcagcggtgcaggctgaggcgttttttcacttctgtcgatgccctcttcgagcaaggattggagaaatagctgtcgtatcattagcgcaacttctcctcgccggatgcaaaagaaaaaccgcccaccgaacagaaaagtctgttcaatgggcggcttattgagattatggaaactttc ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gatcgttattcgatataacgagaaaggtatttcaatctgaaaaaggagtatttccatctcttttgtatcataaccagacttcttagagccggcttatttggaatgattctaaactcctcttcgctgttgaaaacttttttggcacctatttttcggtcacaataccgattttcttagttgtttttaggcctaagatccttctctcttcagcctgcagcggtgcaggctgaggcgttttttcacttctgtcgatgccctcttcgagcaaggattggagaaatagctgtcgtatcattagcgcaacttctcctcgccggatgcaaaagaaaaaccgcccaccgaacagaaaagtctgttcaatgggcggcttattgagattatggaaactttc Subject: NC_002950_PG1694_PG1695|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[1779101,1779579]|479 HSP 1 e-value: 5.0E-14 bit: 81.8 Len: 61 Query Start:760 Query End:820 Subject Strand: NEGATIVE Subject Start: 164 Subject End: 224 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gcttatttggaatgattctaaactcctcttcgctgttgaaaacttttttggcacctatttt............................................................................................................................................................................................................................................... .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gcttatttggaatgattctaaattcttcttcgttgttgaaaatttttttggcacgtatttt............................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-7 bit: 60.0 Len: 66 Query Start:552 Query End:617 Subject Strand: NEGATIVE Subject Start: 367 Subject End: 432 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ctgttttatcagcttacaatatggcattttgggggctgcgattgatatacaaatcattttcgattt.......................................................................................................................................................................................................................................................................................................................................................................................................................................................... .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ctgttttatcaccttacgaaaaggctttttgagggctgcgatttatatacaaaacgttttcgattt.......................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGCAAAUGGGCUUUCACAGACAAACUUUAGUCUAUUUUUUGAUCAGAUUCAAUGAUUUAAGCAAAAGAAAUUGGCUAUCAACAGCUCGCACUUUUCAAUAGCAAGUGUAAUAACGGAACAAAUUUAAGGCAAAAACUGAGAAAUGAGUUCUGAGCUUCGUCUUUUCGAACAGGCUGUUCGAUACCAAUAGCGAUAACAAUAAAAGCGCAGCAACCGGGCCUAUACGGCUGGGAUUUUUGCUACUUUUGUAGAGCGAUAGGGAGUUCUCGACAAUGUAAAUGGUGGUUCUGGAAGGGCUUGUCUGUCGAAUAGAACAGCUUUACGAACGUUUACUAACUACUUAAUAGCAACAGAUAUGAUGCACUCUGACUUCUCUCUUUCUAUACGGUCAUUAGGUGGAUUUCAUGCGGCCUAUACAGGUAUGUACCAUCGCGUGGCAUGUGGACAAAGGAGAUAUUAUUCCAUGAGGAGUAAGCACGAAUAGGCAUUCGAUAGCUUCUAUAACGUAUAAGGCACCCACCCGAUUCUUUCAUAAAUCAGAUGGGUGCCUCUGUUUUAUCAGCUUACAAUAUGGCAUUUUGGGGGCUGCGAUUGAUAUACAAAUCAUUUUCGAUUUCUAUAUAUAUUGAAAACGAUUUAUAUAUAAAUGGAAAAUGGUUUAUAUAUAGAUCGUUAUUCGAUAUAACGAGAAAGGUAUUUCAAUCUGAAAAAGGAGUAUUUCCAUCUCUUUUGUAUCAUAACCAGACUUCUUAGAGCCGGCUUAUUUGGAAUGAUUCUAAACUCCUCUUCGCUGUUGAAAACUUUUUUGGCACCUAUUUUUCGGUCACAAUACCGAUUUUCUUAGUUGUUUUUAGGCCUAAGAUCCUUCUCUCUUCAGCCUGCAGCGGUGCAGGCUGAGGCGUUUUUUCACUUCUGUCGAUGCCCUCUUCGAGCAAGGAUUGGAGAAAUAGCUGUCGUAUCAUUAGCGCAACUUCUCCUCGCCGGAUGCAAAAGAAAAACCGCCCACCGAACAGAAAAGUCUGUUCAAUGGGCGGCUUAUUGAGAUUAUGGAAACUUUC (((((...((((..(((((((.........(((..(((((((...(((((....)))))...))))))).(((((.(((((((((((.((((((.........)))))).....((((((..((((.((.......))...))))..))))))...((((......))))..))))))).)))))))))...)))......((((((((.......(((((((.((((((((((......((((....)))).((((..(((((((...((((.(.(((((((((((((((((((.((((((..((.((.((((((((...((((...((((((...((((((((..(((.((....)).))).....((((...............)))).))))))))...((((((((....((((....))))...))))))))...))))))..(((((...(((((((....)))))))...(((((....)))))....)))))....))))..(((((((((.(.((((........)))).).))))))))))))))))))).))..)))....)))..(((((.......((((((.((((..(((......)))((((...(((((((((((((((((((((((((((........)))))))))))))))))).)))))))))...))))..)))).)))))).((.(((((((.........)))))))...))....))))))))).))))))).)).)))))).).)).))...)))))))..)))).((..(((....)))..)).........(((((......)))))...))))))))))...)))))))...............((((((((((....)))))))))).)))))))).....))))).)).))))..((((.((.((((..((((.....((((.........))))....)))))))).)))))))))))........(((((((..(((((((....)))))))..)))))))..............(....).... (-318.36) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAAAGUUUCCAUAAUCUCAAUAAGCCGCCCAUUGAACAGACUUUUCUGUUCGGUGGGCGGUUUUUCUUUUGCAUCCGGCGAGGAGAAGUUGCGCUAAUGAUACGACAGCUAUUUCUCCAAUCCUUGCUCGAAGAGGGCAUCGACAGAAGUGAAAAAACGCCUCAGCCUGCACCGCUGCAGGCUGAAGAGAGAAGGAUCUUAGGCCUAAAAACAACUAAGAAAAUCGGUAUUGUGACCGAAAAAUAGGUGCCAAAAAAGUUUUCAACAGCGAAGAGGAGUUUAGAAUCAUUCCAAAUAAGCCGGCUCUAAGAAGUCUGGUUAUGAUACAAAAGAGAUGGAAAUACUCCUUUUUCAGAUUGAAAUACCUUUCUCGUUAUAUCGAAUAACGAUCUAUAUAUAAACCAUUUUCCAUUUAUAUAUAAAUCGUUUUCAAUAUAUAUAGAAAUCGAAAAUGAUUUGUAUAUCAAUCGCAGCCCCCAAAAUGCCAUAUUGUAAGCUGAUAAAACAGAGGCACCCAUCUGAUUUAUGAAAGAAUCGGGUGGGUGCCUUAUACGUUAUAGAAGCUAUCGAAUGCCUAUUCGUGCUUACUCCUCAUGGAAUAAUAUCUCCUUUGUCCACAUGCCACGCGAUGGUACAUACCUGUAUAGGCCGCAUGAAAUCCACCUAAUGACCGUAUAGAAAGAGAGAAGUCAGAGUGCAUCAUAUCUGUUGCUAUUAAGUAGUUAGUAAACGUUCGUAAAGCUGUUCUAUUCGACAGACAAGCCCUUCCAGAACCACCAUUUACAUUGUCGAGAACUCCCUAUCGCUCUACAAAAGUAGCAAAAAUCCCAGCCGUAUAGGCCCGGUUGCUGCGCUUUUAUUGUUAUCGCUAUUGGUAUCGAACAGCCUGUUCGAAAAGACGAAGCUCAGAACUCAUUUCUCAGUUUUUGCCUUAAAUUUGUUCCGUUAUUACACUUGCUAUUGAAAAGUGCGAGCUGUUGAUAGCCAAUUUCUUUUGCUUAAAUCAUUGAAUCUGAUCAAAAAAUAGACUAAAGUUUGUCUGUGAAAGCCCAUUUGCAA .....................(((((((((((((((((((....))))))))))))))))))).....(((((...(((.(((((((((((.((((......((((((((..................((((.((((((..(((((((((((.........((((((((((....))))))))))...(((..((((.(((((((((................(((((......)))))...((((((((((.....((.....)).(((..(((((((...(((((((((......((((((((......)).))))))....(((((..((((.(((.....)))..)))).((((((..........(((((((.....)))))))....((((((((.(((((((.((((.((((((.((.(....).)).)))))).)))).))))))).))))))))))))))....................)))))((((.((((....((((((((((((((((((......)))))))))))))))))).....))))...))))...)))))...))))......))))))).((((.(((........))))))).......)))..))))....)))))).))))))...))).))))........((((.((((...((....(((.((((((((.........((((((((.........(((((.((((.....(((..(((.......)))..))).......)))).....))))).((((.(((............))).)))).))))))))......((((((........)))))).)))))))).)))....)).)))))))).((((((.....))))))..........))).....))))))))..)))..))))))...))))...........(((((.........)))))..)))))))).)))))))))))))))..........((((......))))...((((((........))))))....)))....))))) (-298.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 877 120 71 211 162 NC_002950:PG1606|5end_ion_transporter_1687625..1687855_NEGATIVE 565 810 761 84 35 NC_002950:PG1357|5end_hypothetical_protein_1436458..1436688_NEGATIVE 546 810 761 54 5 NC_002950:PG0995|5end_hypothetical_protein_1055585..1055815_NEGATIVE 531 810 761 121 72 NC_002950:PG0609|5end_hypothetical_protein_663183..663413_POSITIVE 526 810 761 66 17 NC_002950:PG0773|5end_hypothetical_protein_825131..825361_POSITIVE 492 600 552 187 139 NC_002950:PG1695|5end_hypothetical_protein_1779440..1779670_POSITIVE 443 928 881 96 43 NC_002950:PG2114|5end_hypothetical_protein_2224158..2224388_NEGATIVE 418 650 601 137 89 NC_002950:PG0371|5end_hypothetical_protein_404043..404273_POSITIVE 416 901 864 50 2 NC_002950:PG0163|5end_diphosphate--fructose-6-phosphate_1-phosphotransferase_186885..187115_NEGATIVE 402 679 632 122 76 NC_002950:PG1222|5end_hypothetical_protein_1299392..1299622_NEGATIVE 398 990 941 62 10 NC_002950:PG1310|5end_exsB_protein_1389084..1389314_POSITIVE 394 902 869 56 18 NC_002950:PG2029|5end_hypothetical_protein_2126543..2126773_POSITIVE 394 902 862 188 141 NC_002950:PG0158|5end_competence_protein_F-related_protein_180175..180405_POSITIVE 390 916 871 156 97 NC_002950:PG0065|5end_efflux_transporter,_RND_family,_MFP_subunit_81187..81417_NEGATIVE 370 905 861 204 161 NC_002950:PG1498|5end_hypothetical_protein_1574656..1574886_POSITIVE 366 930 881 190 131 NC_002950:PG0657|5end_Maf-like_protein_707782..708012_NEGATIVE 364 617 574 100 56 NC_002950:PG1574|5end_hypothetical_protein_1652613..1652843_NEGATIVE 363 930 886 64 1 NC_002950:PG0062|5end_TPR_domain_protein_73688..73918_POSITIVE 362 952 914 64 25 NC_002950:PG1977|5end_hypothetical_protein_2066693..2066923_NEGATIVE 360 916 875 213 182 NC_002950:PG2101|5end_hypothetical_protein_2211273..2211503_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 624 810 761 55 6 NC_002950:PG1868|3end_hypothetical_protein_1965098..1965248_NEGATIVE 526 810 761 59 10 NC_002950:PG0772|3end_hypothetical_protein_825204..825354_POSITIVE 493 269 226 61 15 NC_002950:PG1695|3end_hypothetical_protein_1779711..1779861_POSITIVE 434 673 631 44 2 NC_002950:PG0773|3end_hypothetical_protein_825348..825498_POSITIVE 423 557 512 64 16 NC_002950:PG1866|3end_hypothetical_protein_1964736..1964886_POSITIVE 397 528 481 150 93 NC_002950:PG0492|3end_hypothetical_protein_534408..534558_NEGATIVE 394 902 869 49 11 NC_002950:PG2028|3end_ebsC_protein_2126616..2126766_POSITIVE 394 675 631 64 14 NC_002950:PG1357|3end_hypothetical_protein_1436327..1436477_NEGATIVE 393 919 872 88 34 NC_002950:PG1411|3end_potassium_uptake_protein_TrkA,_putative_1493481..1493631_NEGATIVE 390 916 871 79 20 NC_002950:PG0066|3end_hypothetical_protein_81264..81414_NEGATIVE 388 558 511 59 11 NC_002950:PG1274|3end_elongation_factor_P_1352489..1352639_POSITIVE 380 919 871 56 3 NC_002950:PG1702|3end_DNA_gyrase,_B_subunit_1787946..1788096_NEGATIVE 378 670 625 51 6 NC_002950:PG0609|3end_hypothetical_protein_663355..663505_POSITIVE 374 938 891 112 57 NC_002950:PG0132|3end_hypothetical_protein_153054..153204_POSITIVE 370 905 861 103 60 NC_002950:PG1497|3end_DNA-binding_protein,_histone-like_family_1574635..1574785_POSITIVE 368 1010 966 77 20 NC_002950:PG0164|3end_hypothetical_protein_187081..187231_NEGATIVE 366 698 651 50 5 NC_002950:PG1167|3end_hypothetical_protein_1248644..1248794_POSITIVE 366 930 881 126 73 NC_002950:PG0604|3end_4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase_661211..661361_POSITIVE 364 895 853 104 61 NC_002950:PG1391|3end_hypothetical_protein_1472961..1473111_POSITIVE 363 902 863 110 68 NC_002950:PG1675|3end_hypothetical_protein_1760360..1760510_POSITIVE