Origin IGS: acttcgcgcttaaaccctgcaggatgctatatgtctatctcaacatgagatggtgtgcataatcttcattagtatttagcaagaaaagatatagaaaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttttctatatcttttcttgctaaatactaatgaagattatgcacaccatctcatgttgagatagacatatagcatcctgcagggtttaagcgcgaagt Mask Tandem Repeat Region ================================================ acttcgcgcttaaaccctgcaggatgctatatgtctatctcaacatgagatggtgtgcataatcttcattagtatttagcaagaaaagatatagaaaaaa Find is-nt database================================================ Query_seq: PG1578:PG1579|PG1578:PG1579:quinolinate synthetase:ATPase, MoxR family:->->:1656533..1656632 100 acttcgcgcttaaaccctgcaggatgctatatgtctatctcaacatgagatggtgtgcataatcttcattagtatttagcaagaaaagatatagaaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1578:PG1579|PG1578:PG1579:quinolinate synthetase:ATPase, MoxR family:->->:1656533..1656632 100 acttcgcgcttaaaccctgcaggatgctatatgtctatctcaacatgagatggtgtgcataatcttcattagtatttagcaagaaaagatatagaaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1578:PG1579|PG1578:PG1579:quinolinate synthetase:ATPase, MoxR family:->->:1656533..1656632 100 acttcgcgcttaaaccctgcaggatgctatatgtctatctcaacatgagatggtgtgcataatcttcattagtatttagcaagaaaagatatagaaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acttcgcgcttaaaccctgcaggatgctatatgtctatctcaacatgagatggtgtgcataatcttcattagtatttagcaagaaaagatatagaaaaaa Predict ORF larger than 30AA ================================================ acttcgcgcttaaaccctgcaggatgctatatgtctatctcaacatgagatggtgtgcataatcttcattagtatttagcaagaaaagatatagaaaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acttcgcgcttaaaccctgcaggatgctatatgtctatctcaacatgagatggtgtgcataatcttcattagtatttagcaagaaaagatatagaaaaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG1578:PG1579|PG1578:PG1579:quinolinate synthetase:ATPase, MoxR family:->->:1656533..1656632 100 acttcgcgcttaaaccctgcaggatgctatatgtctatctcaacatgagatggtgtgcataatcttcattagtatttagcaagaaaagatatagaaaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 100 Len: 100 Subject: NC_002950_PG1578_PG1579|quinolinate synthetase:ATPase, MoxR family|POSITIVE:POSITIVE|[1656533,1656632]|100 HSP 1 e-value: 2.0E-50 bit: 198.0 Len: 100 Query Start:1 Query End:100 Subject Strand: POSITIVE Subject Start: 1 Subject End: 100 acttcgcgcttaaaccctgcaggatgctatatgtctatctcaacatgagatggtgtgcataatcttcattagtatttagcaagaaaagatatagaaaaaa acttcgcgcttaaaccctgcaggatgctatatgtctatctcaacatgagatggtgtgcataatcttcattagtatttagcaagaaaagatatagaaaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACUUCGCGCUUAAACCCUGCAGGAUGCUAUAUGUCUAUCUCAACAUGAGAUGGUGUGCAUAAUCUUCAUUAGUAUUUAGCAAGAAAAGAUAUAGAAAAAA ..(((...(((....(.(((..(((((((((((((((((((.....)))))))...)))))........)))))))..))).)..))).....))).... (-20.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUUCUAUAUCUUUUCUUGCUAAAUACUAAUGAAGAUUAUGCACACCAUCUCAUGUUGAGAUAGACAUAUAGCAUCCUGCAGGGUUUAAGCGCGAAGU ..............((((.(((((((..((.(.((.(..((((....(.(((((.....))))).).))))...).)).)..))..))).)))).)))). (-13.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 336 49 2 94 44 NC_002950:PG2225|5end_hypothetical_protein_2339440..2339670_POSITIVE 298 50 1 46 2 NC_002950:PG0506|5end_arginine-specific_cysteine_proteinase_545335..545565_POSITIVE 289 27 2 37 14 NC_002950:PG0163|5end_diphosphate--fructose-6-phosphate_1-phosphotransferase_186885..187115_NEGATIVE 281 53 15 194 157 NC_002950:PG1307|5end_glucose-inhibited_division_protein_B_1387849..1388079_NEGATIVE 277 47 1 224 173 NC_002950:PG0795|5end_hypothetical_protein_848813..849043_POSITIVE 273 58 16 71 18 NC_002950:PG2015|5end_CRISPR-associated_protein_Cas4_2106014..2106244_NEGATIVE 270 37 2 39 7 NC_002950:PG0594|5end_RNA_polymerase_sigma-70_factor_652048..652278_POSITIVE 268 60 15 175 128 NC_002950:PG1701|5end_glutamine_amidotransferase,_class_II/dipeptidase_1787953..1788183_NEGATIVE 265 49 1 102 35 NC_002950:PG1541|5end_2-amino-4-hydroxy-6-_hydroxymethyldihydropteridine_pyrophosphokinase_1620326..1620556_POSITIVE 264 53 16 139 107 NC_002950:PG0984|5end_hypothetical_protein_1046919..1047149_NEGATIVE 259 68 21 90 35 NC_002950:PG1695|5end_hypothetical_protein_1779440..1779670_POSITIVE 258 48 1 220 171 NC_002950:PG1610|5end_hypothetical_protein_1690549..1690779_NEGATIVE 258 67 21 178 139 NC_002950:PG0197|5end_hypothetical_protein_236204..236434_NEGATIVE 257 59 14 212 173 NC_002950:PG1654|5end_D-alanyl-D-alanine_dipeptidase_1736458..1736688_POSITIVE 253 59 15 204 153 NC_002950:PG2021|5end_hypothetical_protein_2114852..2115082_NEGATIVE 251 67 21 82 38 NC_002950:PG1721|5end_ribonuclease_R_1810284..1810514_POSITIVE 251 59 14 74 21 NC_002950:PG1719|5end_ABC_transporter,_ATP-binding_protein,_MsbA_family_1806749..1806979_POSITIVE 251 58 13 174 125 NC_002950:PG0059|5end_hypothetical_protein_71526..71756_POSITIVE 251 54 16 46 3 NC_002950:PG0017|5end_sensor_histidine_kinase_18307..18537_POSITIVE 250 49 8 43 3 NC_002950:PG0214|5end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_250963..251193_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 336 49 2 74 24 NC_002950:PG2224|3end_hypothetical_protein_2339500..2339650_POSITIVE 268 42 1 126 82 NC_002950:PG1570|3end_rhodanese-like_domain_protein_1648582..1648732_NEGATIVE 265 49 1 105 38 NC_002950:PG1540|3end_S-adenosylmethionine:tRNA_ribosyltransferase-isomerase_1620409..1620559_POSITIVE 257 59 14 132 93 NC_002950:PG1653|3end_hypothetical_protein_1736458..1736608_POSITIVE 255 59 15 149 99 NC_002950:PG0587|3end_yadS_protein_644612..644762_NEGATIVE 249 58 16 65 28 NC_002950:PG1430|3end_TPR_domain_protein_1515114..1515264_NEGATIVE 248 57 15 112 64 NC_002950:PG0606|3end_hypothetical_protein_662451..662601_NEGATIVE 248 45 2 143 99 NC_002950:PG0157|3end_regulatory_protein_RecX_180271..180421_POSITIVE 247 59 16 87 33 NC_002950:PG1743|3end_2-dehydro-3-deoxyphosphooctonate_aldolase_1827530..1827680_NEGATIVE 246 39 5 72 34 NC_002950:PG0155|3end_riboflavin_biosynthesis_protein_RibD_177861..178011_NEGATIVE 241 58 15 126 82 NC_002950:PG1814|3end_DNA_primase_1908606..1908756_NEGATIVE 240 56 16 80 21 NC_002950:PG1217|3end_hypothetical_protein_1295987..1296137_POSITIVE 237 60 17 113 56 NC_002950:PG1049|3end_hypothetical_protein_1117345..1117495_POSITIVE 232 43 1 67 23 NC_002950:PG1988|3end_hypothetical_protein_2077425..2077575_NEGATIVE 231 46 2 91 44 NC_002950:PG1782|3end_hypothetical_protein_1871961..1872111_POSITIVE 231 58 24 50 5 NC_002950:PG0903|3end_hypothetical_protein_969098..969248_NEGATIVE 231 52 16 100 67 NC_002950:PG0413|3end_hypothetical_protein_451437..451587_NEGATIVE 229 67 22 50 7 NC_002950:PG0809|3end_hypothetical_protein_865064..865214_NEGATIVE 228 60 15 100 52 NC_002950:PG1641|3end_phosphotyrosine_protein_phosphatase_1721260..1721410_NEGATIVE 227 47 1 147 93 NC_002950:PG1328|3end_CoA_ligase_family_protein_1406623..1406773_NEGATIVE