Origin IGS: agttataatggattattccttttgatgactaaataagcttcaaaggtaataaaaatattgaatgtgtactctgtctgagaactaaacaaactccggaaatttaagattgcgaataagccctccctccaaaaaagagggtgtaaaacatttagaaccaaacctttcatcatgagcagtaaaactctctcaaagttcatatcctcaaatcccatcatccagtttgtaaaaagacttcaatctgcatgtgctttttcgtttcaaaaacgaatatagtacatatatactaatataaaagtctcgaaaaagaatcggatcgccaagaattcttctcaaaaaataatttttttccacaactacgagaaattcataaacaactaatatacaattatatacaagtgcatgttagaggatagatgtgcacaaaagcaaagttcttcacgcttcagaggattgactttaatagattttcatacctttgcatagtacaatcataccaataaattacaaatatggaaaattgtagtattcaaagagacttcctcagcaaacttgcagaggatgctgatgcacacaacatcaagaaaattgaaaatgcttttgttcataaagtgaacaaagattatgtgttgatttctgaaataaaggaagttcccgaaaattacttgaataatgatggggaaaaagatgaaaattgcaagtttttgtacttagttgcctttcctaagcatgattgtgtattttgttttgaccatgaattagaccacctccctttcatgatgactatcgagcttattaggcaagctggaatagcgattgggcatgtaatccacaaaataccactaaaaggttttacaaacataatggattgcatgaatctgaatgttctaaaatttattgagttggatgttcctctactaatcatcatcaatgacataatgttgaagaataaacccagcagacaagaaagaatgatgcatttctacttatatcaaaagaactcactatgtgcttctgttgacataaatgcgtccattatggaaaaagatatatatcgacgattaagattgagcagccgggcagagttaatacaaaacacttcattggacaaagtcccaacgactaatttgcaaatgcttaacaaaagaatttcaatcaaataacaacgatttctgaca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgtcagaaatcgttgttatttgattgaaattcttttgttaagcatttgcaaattagtcgttgggactttgtccaatgaagtgttttgtattaactctgcccggctgctcaatcttaatcgtcgatatatatctttttccataatggacgcatttatgtcaacagaagcacatagtgagttcttttgatataagtagaaatgcatcattctttcttgtctgctgggtttattcttcaacattatgtcattgatgatgattagtagaggaacatccaactcaataaattttagaacattcagattcatgcaatccattatgtttgtaaaaccttttagtggtattttgtggattacatgcccaatcgctattccagcttgcctaataagctcgatagtcatcatgaaagggaggtggtctaattcatggtcaaaacaaaatacacaatcatgcttaggaaaggcaactaagtacaaaaacttgcaattttcatctttttccccatcattattcaagtaattttcgggaacttcctttatttcagaaatcaacacataatctttgttcactttatgaacaaaagcattttcaattttcttgatgttgtgtgcatcagcatcctctgcaagtttgctgaggaagtctctttgaatactacaattttccatatttgtaatttattggtatgattgtactatgcaaaggtatgaaaatctattaaagtcaatcctctgaagcgtgaagaactttgcttttgtgcacatctatcctctaacatgcacttgtatataattgtatattagttgtttatgaatttctcgtagttgtggaaaaaaattattttttgagaagaattcttggcgatccgattctttttcgagacttttatattagtatatatgtactatattcgtttttgaaacgaaaaagcacatgcagattgaagtctttttacaaactggatgatgggatttgaggatatgaactttgagagagttttactgctcatgatgaaaggtttggttctaaatgttttacaccctcttttttggagggagggcttattcgcaatcttaaatttccggagtttgtttagttctcagacagagtacacattcaatatttttattacctttgaagcttatttagtcatcaaaaggaataatccattataact Mask Tandem Repeat Region ================================================ agttataatggattattccttttgatgactaaataagcttcaaaggtaataaaaatattgaatgtgtactctgtctgagaactaaacaaactccggaaatttaagattgcgaataagccctccctccaaaaaagagggtgtaaaacatttagaaccaaacctttcatcatgagcagtaaaactctctcaaagttcatatcctcaaatcccatcatccagtttgtaaaaagacttcaatctgcatgtgctttttcgtttcaaaaacgaatatagtacatatatactaatataaaagtctcgaaaaagaatcggatcgccaagaattcttctcaaaaaataatttttttccacaactacgagaaattcataaacaactaatatacaattatatacaagtgcatgttagaggatagatgtgcacaaaagcaaagttcttcacgcttcagaggattgactttaatagattttcatacctttgcatagtacaatcataccaataaattacaaatatggaaaattgtagtattcaaagagacttcctcagcaaacttgcagaggatgctgatgcacacaacatcaagaaaattgaaaatgcttttgttcataaagtgaacaaagattatgtgttgatttctgaaataaaggaagttcccgaaaattacttgaataatgatggggaaaaagatgaaaattgcaagtttttgtacttagttgcctttcctaagcatgattgtgtattttgttttgaccatgaattagaccacctccctttcatgatgactatcgagcttattaggcaagctggaatagcgattgggcatgtaatccacaaaataccactaaaaggttttacaaacataatggattgcatgaatctgaatgttctaaaatttattgagttggatgttcctctactaatcatcatcaatgacataatgttgaagaataaacccagcagacaagaaagaatgatgcatttctacttatatcaaaagaactcactatgtgcttctgttgacataaatgcgtccattatggaaaaagatatatatcgacgattaagattgagcagccgggcagagttaatacaaaacacttcattggacaaagtcccaacgactaatttgcaaatgcttaacaaaagaatttcaatcaaataacaacgatttctgaca Find is-nt database================================================ Query_seq: PG1501:PG1503|PG1501:PG1503:transcriptional regulator, tetR family:LytB-related protein:<-->:1579190..1580364 1175 agttataatggattattccttttgatgactaaataagcttcaaaggtaataaaaatattgaatgtgtactctgtctgagaactaaacaaactccggaaatttaagattgcgaataagccctccctccaaaaaagagggtgtaaaacatttagaaccaaacctttcatcatgagcagtaaaactctctcaaagttcatatcctcaaatcccatcatccagtttgtaaaaagacttcaatctgcatgtgctttttcgtttcaaaaacgaatatagtacatatatactaatataaaagtctcgaaaaagaatcggatcgccaagaattcttctcaaaaaataatttttttccacaactacgagaaattcataaacaactaatatacaattatatacaagtgcatgttagaggatagatgtgcacaaaagcaaagttcttcacgcttcagaggattgactttaatagattttcatacctttgcatagtacaatcataccaataaattacaaatatggaaaattgtagtattcaaagagacttcctcagcaaacttgcagaggatgctgatgcacacaacatcaagaaaattgaaaatgcttttgttcataaagtgaacaaagattatgtgttgatttctgaaataaaggaagttcccgaaaattacttgaataatgatggggaaaaagatgaaaattgcaagtttttgtacttagttgcctttcctaagcatgattgtgtattttgttttgaccatgaattagaccacctccctttcatgatgactatcgagcttattaggcaagctggaatagcgattgggcatgtaatccacaaaataccactaaaaggttttacaaacataatggattgcatgaatctgaatgttctaaaatttattgagttggatgttcctctactaatcatcatcaatgacataatgttgaagaataaacccagcagacaagaaagaatgatgcatttctacttatatcaaaagaactcactatgtgcttctgttgacataaatgcgtccattatggaaaaagatatatatcgacgattaagattgagcagccgggcagagttaatacaaaacacttcattggacaaagtcccaacgactaatttgcaaatgcttaacaaaagaatttcaatcaaataacaacgatttctgaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1501:PG1503|PG1501:PG1503:transcriptional regulator, tetR family:LytB-related protein:<-->:1579190..1580364 1175 agttataatggattattccttttgatgactaaataagcttcaaaggtaataaaaatattgaatgtgtactctgtctgagaactaaacaaactccggaaatttaagattgcgaataagccctccctccaaaaaagagggtgtaaaacatttagaaccaaacctttcatcatgagcagtaaaactctctcaaagttcatatcctcaaatcccatcatccagtttgtaaaaagacttcaatctgcatgtgctttttcgtttcaaaaacgaatatagtacatatatactaatataaaagtctcgaaaaagaatcggatcgccaagaattcttctcaaaaaataatttttttccacaactacgagaaattcataaacaactaatatacaattatatacaagtgcatgttagaggatagatgtgcacaaaagcaaagttcttcacgcttcagaggattgactttaatagattttcatacctttgcatagtacaatcataccaataaattacaaatatggaaaattgtagtattcaaagagacttcctcagcaaacttgcagaggatgctgatgcacacaacatcaagaaaattgaaaatgcttttgttcataaagtgaacaaagattatgtgttgatttctgaaataaaggaagttcccgaaaattacttgaataatgatggggaaaaagatgaaaattgcaagtttttgtacttagttgcctttcctaagcatgattgtgtattttgttttgaccatgaattagaccacctccctttcatgatgactatcgagcttattaggcaagctggaatagcgattgggcatgtaatccacaaaataccactaaaaggttttacaaacataatggattgcatgaatctgaatgttctaaaatttattgagttggatgttcctctactaatcatcatcaatgacataatgttgaagaataaacccagcagacaagaaagaatgatgcatttctacttatatcaaaagaactcactatgtgcttctgttgacataaatgcgtccattatggaaaaagatatatatcgacgattaagattgagcagccgggcagagttaatacaaaacacttcattggacaaagtcccaacgactaatttgcaaatgcttaacaaaagaatttcaatcaaataacaacgatttctgaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1501:PG1503|PG1501:PG1503:transcriptional regulator, tetR family:LytB-related protein:<-->:1579190..1580364 1175 agttataatggattattccttttgatgactaaataagcttcaaaggtaataaaaatattgaatgtgtactctgtctgagaactaaacaaactccggaaatttaagattgcgaataagccctccctccaaaaaagagggtgtaaaacatttagaaccaaacctttcatcatgagcagtaaaactctctcaaagttcatatcctcaaatcccatcatccagtttgtaaaaagacttcaatctgcatgtgctttttcgtttcaaaaacgaatatagtacatatatactaatataaaagtctcgaaaaagaatcggatcgccaagaattcttctcaaaaaataatttttttccacaactacgagaaattcataaacaactaatatacaattatatacaagtgcatgttagaggatagatgtgcacaaaagcaaagttcttcacgcttcagaggattgactttaatagattttcatacctttgcatagtacaatcataccaataaattacaaatatggaaaattgtagtattcaaagagacttcctcagcaaacttgcagaggatgctgatgcacacaacatcaagaaaattgaaaatgcttttgttcataaagtgaacaaagattatgtgttgatttctgaaataaaggaagttcccgaaaattacttgaataatgatggggaaaaagatgaaaattgcaagtttttgtacttagttgcctttcctaagcatgattgtgtattttgttttgaccatgaattagaccacctccctttcatgatgactatcgagcttattaggcaagctggaatagcgattgggcatgtaatccacaaaataccactaaaaggttttacaaacataatggattgcatgaatctgaatgttctaaaatttattgagttggatgttcctctactaatcatcatcaatgacataatgttgaagaataaacccagcagacaagaaagaatgatgcatttctacttatatcaaaagaactcactatgtgcttctgttgacataaatgcgtccattatggaaaaagatatatatcgacgattaagattgagcagccgggcagagttaatacaaaacacttcattggacaaagtcccaacgactaatttgcaaatgcttaacaaaagaatttcaatcaaataacaacgatttctgaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 3 Min: 576 Max: 1142 Len: 567 Subject: UniRef90_Q0YMX5 Cluster: Hypothetical protein precursor; n=1; Geobacter sp. FRC-32|Rep: Hypothetical protein precursor - Geobacter sp. FRC-32 HSP 1 e-value: 2.0E-8 bit: 63.2 Len: 504 Query Start:576 Query End:1079 Subject Strand: null Subject Start: 17 Subject End: 174 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... I K K I E N A F V H K V N K D Y V L I S E I K E V P E N Y L N N D G E K D E N C K F L Y L V A F P K H D C V F C F D H E L D H L P F M M T I E L I R Q A G I A I G H V I H K I P L K G F T N I M D C M N L N V L K F I E L D V P L L I I I N D I M L K N K P S R Q E R M M H - - F Y L Y Q K N S L C A S V D I N A S I M E K D I Y R R L R L S S R A ................................................................................................ ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... I R K I D R R F V H K A K D E N V V I S D I C R R E D L - - - - - G P D I Y Q C F L T Y D D Q L P - - - - - F F F E H Y L D H V P G L L V I E A A R Q M G T A T V H K F Y D V D F D T I F - I L D V V N C S F T N F L E C Q E P V V I Q I - A I I A P N G P D R V R K A F S S V V T I F Q H S E S K G T I E F N C T C I P K K L F A R M R R T A A A ................................................................................................ Subject: UniRef90_Q4AMB0 Cluster: Hypothetical protein; n=1; Chlorobium phaeobacteroides BS1|Rep: Hypothetical protein - Chlorobium phaeobacteroides BS1 HSP 1 e-value: 2.0E-6 bit: 56.2 Len: 558 Query Start:585 Query End:1142 Subject Strand: null Subject Start: 159 Subject End: 330 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ I E N A F V H K V N K D Y V L I S E I K E V P E N Y L N N D G E K D E N C K F L Y L V A F P K H D C V F C F D H E L D H L P F M M T I E L I R Q A G I A I G H V I H K I P - L K G F T N I M D C M N L N V L K F I E L D V P L L I - I I N D I M L K N K P S R Q E R M M H F Y L Y Q K N S L C A S V D I N A S I - M E K D I Y R R L R L S S R A E L I Q N T S L D K V P T T N L Q M L N K ................................. ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ I D S A I I H K K N P A N A L L S E - - - - - - P Y F A G D - - - - - - - - I V Y Y N M F - M H S E E L R F D H E S D H V Q G M L L L E A M R Q A S I A T T H V C S G L P A I G G M T L I S Y C T N F - - Y N Y I E H D A P V I I R A Y S S Y T V P E E M G E K D S Y A I C H V F Q W G K L C A E A V L D A V V F M N S E R Y D R H R N R T E K L S L R N K R L F F S K V Q G I E T I N Q ................................. Subject: UniRef90_Q44RC0 Cluster: Hypothetical protein; n=1; Chlorobium limicola DSM 245|Rep: Hypothetical protein - Chlorobium limicola DSM 245 HSP 1 e-value: 4.0E-6 bit: 55.5 Len: 489 Query Start:585 Query End:1073 Subject Strand: null Subject Start: 157 Subject End: 305 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ I E N A F V H K V N K D Y V L I S E I K E V P E N Y L N N D G E K D E N C K F L Y L V A F P K H D C V F C F D H E L D H L P F M M T I E L I R Q A G I A I G H V I H K I P L K G F T N I M D C M N L N V L K F I E L D V P L L I - I I N D I M L K N K P S R Q E R M M H F Y L Y Q K N S L C A S V D I N A S I - M E K D I Y R R L R L S S ...................................................................................................... ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ I N R E F V H K K S I R N A L I S E - - P L I E G - - - - - - - - - - - - K F L S F N M F D A I D - E F R F D H E S D H F Q G M L I L E A M R Q A S I A A A H I L G Q L P L D G G M T L L S - Y D T K F Y N Y I E N T S P V V L R T Y S N F S C T G D D E E R E S Y A L C Q L Y Q W G K L C T E A T L N A Y I F M S K E G Y A T H R I R S ...................................................................................................... agttataatggattattccttttgatgactaaataagcttcaaaggtaataaaaatattgaatgtgtactctgtctgagaactaaacaaactccggaaatttaagattgcgaataagccctccctccaaaaaagagggtgtaaaacatttagaaccaaacctttcatcatgagcagtaaaactctctcaaagttcatatcctcaaatcccatcatccagtttgtaaaaagacttcaatctgcatgtgctttttcgtttcaaaaacgaatatagtacatatatactaatataaaagtctcgaaaaagaatcggatcgccaagaattcttctcaaaaaataatttttttccacaactacgagaaattcataaacaactaatatacaattatatacaagtgcatgttagaggatagatgtgcacaaaagcaaagttcttcacgcttcagaggattgactttaatagattttcatacctttgcatagtacaatcataccaataaattacaaatatggaaaattgtagtattcaaagagacttcctcagcaaacttgcagaggatgctgatgcacacaacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagaatttcaatcaaataacaacgatttctgaca Predict ORF larger than 30AA ================================================ Protein_Len: 55 Strand: + Start: 106 End: 270 ......................................................................................................... M A N K P S L Q K R G C K T F R T K P F I M S S K T L S K F I S S N P I I Q F V K R L Q S A C A F S F Q K R I ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 33 Strand: + Start: 277 End: 375 .................................................................................................................................................................................................................................................................................... M Y T N I K V S K K N R I A K N S S Q K I I F F H N Y E K F I N N ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 32 Strand: + Start: 385 End: 480 ................................................................................................................................................................................................................................................................................................................................................................................................ M I Y K C M L E D R C A Q K Q S S S R F R G L T L I D F H T F A ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 34 Strand: + Start: 508 End: 609 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M W K I V V F K E T S S A N L Q R M L M H T T S R K L K M L L F I K ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 501 End: 1157 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M T N M E N C S I Q R D F L S K L A E D A D A H N I K K I E N A F V H K V N K D Y V L I S E I K E V P E N Y L N N D G E .................. Protein_Len: 55 Strand: - Start: 992 End: 1156 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... F F E S H A E T S M F A D M I S F S I Y R R N L N L L R A S N I C F V E N S L T G V V L K C I S L L L I E I M ................... Protein_Len: 60 Strand: - Start: 662 End: 973 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... L C A P I A I P C T I W L I G S F P K V F M I S Q M F R F T R F N I S N S T G R S I M M L S M I N F F L G L L C S L I M .......................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 492 End: 692 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... F I T T N L S V E E A F K C L I S I C V V D L F N F I S K N M F H V F I I H Q N R F Y L F N G F I V Q I I I P F F I F M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 59 Strand: - Start: 205 End: 381 ............................................................................................................................................................................................................ I G D D L K Y F S K L R C T S K R K L F S Y L V Y I S I Y F D R F L I P D G L I R R L F Y N K E V V V L F E Y V V L M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 6 End: 215 ..... A E F T I F I N F T Y E T Q S S F L S R F N L I A F L G E R W F L P H L V N L V L G K M M L L L V R E F N M D E F G M M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ agttataatggattattccttttgatgactaaataagcttcaaaggtaataaaaatattgaatgtgtactctgtctgagaactaaacaaactccggaaatttaagattgcgaataagccctccctccaaaaaagagggtgtaaaacatttagaaccaaacctttcatcatgagcagtaaaactctctcaaagttcatatcctcaaatcccatcatccagtttgtaaaaagacttcaatctgcatgtgctttttcgtttcaaaaacgaatatagtacatatatactaatataaaagtctcgaaaaagaatcggatcgccaagaattcttctcaaaaaataatttttttccacaactacgagaaattcataaacaactaatatacaattatatacaagtgcatgttagaggatagatgtgcacaaaagcaaagttcttcacgcttcagaggattgactttaatagattttcatacctttgcatagtacaatcataccaataaattacaaatatggaaaattgtagtattcaaagagacttcctcagcaaacttgcagaggatgctgatgcacacaacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagaatttcaatcaaataacaacgatttctgaca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 87 Start: 488 End: 517 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTTCCATATTTGTAATTTATTGGTATGAT.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agttataatggattattccttttgatgactaaataagcttcaaaggtaataaaaatattgaatgtgtactctgtctgagaactaaacaaactccggaaatttaagattgcgaataagccctccctccaaaaaagagggtgtaaaacatttagaaccaaacctttcatcatgagcagtaaaactctctcaaagttcatatcctcaaatcccatcatccagtttgtaaaaagacttcaatctgcatgtgctttttcgtttcaaaaacgaatatagtacatatatactaatataaaagtctcgaaaaagaatcggatcgccaagaattcttctcaaaaaataatttttttccacaactacgagaaattcataaacaactaatatacaattatatacaagtgcatgttagaggatagatgtgcacaaaagcaaagttcttcacgcttcagaggattgactttaatagattttcatacctttgcatagtacaatcataccaataaattacaaatatggaaaattgtagtattcaaagagacttcctcagcaaacttgcagaggatgctgatgcacacaacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagaatttcaatcaaataacaacgatttctgaca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG1501:PG1503|PG1501:PG1503:transcriptional regulator, tetR family:LytB-related protein:<-->:1579190..1580364 1175 agttataatggattattccttttgatgactaaataagcttcaaaggtaataaaaatattgaatgtgtactctgtctgagaactaaacaaactccggaaatttaagattgcgaataagccctccctccaaaaaagagggtgtaaaacatttagaaccaaacctttcatcatgagcagtaaaactctctcaaagttcatatcctcaaatcccatcatccagtttgtaaaaagacttcaatctgcatgtgctttttcgtttcaaaaacgaatatagtacatatatactaatataaaagtctcgaaaaagaatcggatcgccaagaattcttctcaaaaaataatttttttccacaactacgagaaattcataaacaactaatatacaattatatacaagtgcatgttagaggatagatgtgcacaaaagcaaagttcttcacgcttcagaggattgactttaatagattttcatacctttgcatagtacaatcataccaataaattacaaatatggaaaattgtagtattcaaagagacttcctcagcaaacttgcagaggatgctgatgcacacaacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagaatttcaatcaaataacaacgatttctgaca Intra-Species Hit: Count: 1 Min: 1 Max: 1175 Len: 1175 Subject: NC_002950_PG1501_PG1503|transcriptional regulator, tetR family:LytB-related protein|NEGATIVE:POSITIVE|[1579190,1580364]|1175 HSP 1 e-value: 0.0 bit: 1098.0 Len: 575 Query Start:1 Query End:575 Subject Strand: POSITIVE Subject Start: 1 Subject End: 575 agttataatggattattccttttgatgactaaataagcttcaaaggtaataaaaatattgaatgtgtactctgtctgagaactaaacaaactccggaaatttaagattgcgaataagccctccctccaaaaaagagggtgtaaaacatttagaaccaaacctttcatcatgagcagtaaaactctctcaaagttcatatcctcaaatcccatcatccagtttgtaaaaagacttcaatctgcatgtgctttttcgtttcaaaaacgaatatagtacatatatactaatataaaagtctcgaaaaagaatcggatcgccaagaattcttctcaaaaaataannnnnnnccacaactacgagaaattcataaacaactaatatacaattatatacaagtgcatgttagaggatagatgtgcacaaaagcaaagttcttcacgcttcagaggattgactttaatagattttcatacctttgcatagtacaatcataccaataaattacaaatatggaaaattgtagtattcaaagagacttcctcagcaaacttgcagaggatgctgatgcacacaac........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ agttataatggattattccttttgatgactaaataagcttcaaaggtaataaaaatattgaatgtgtactctgtctgagaactaaacaaactccggaaatttaagattgcgaataagccctccctccaaaaaagagggtgtaaaacatttagaaccaaacctttcatcatgagcagtaaaactctctcaaagttcatatcctcaaatcccatcatccagtttgtaaaaagacttcaatctgcatgtgctttttcgtttcaaaaacgaatatagtacatatatactaatataaaagtctcgaaaaagaatcggatcgccaagaattcttctcaaaaaataatttttttccacaactacgagaaattcataaacaactaatatacaattatatacaagtgcatgttagaggatagatgtgcacaaaagcaaagttcttcacgcttcagaggattgactttaatagattttcatacctttgcatagtacaatcataccaataaattacaaatatggaaaattgtagtattcaaagagacttcctcagcaaacttgcagaggatgctgatgcacacaac........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ HSP 2 e-value: 3.0E-9 bit: 65.9 Len: 33 Query Start:1143 Query End:1175 Subject Strand: POSITIVE Subject Start: 1143 Subject End: 1175 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agaatttcaatcaaataacaacgatttctgaca ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agaatttcaatcaaataacaacgatttctgaca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUUAUAAUGGAUUAUUCCUUUUGAUGACUAAAUAAGCUUCAAAGGUAAUAAAAAUAUUGAAUGUGUACUCUGUCUGAGAACUAAACAAACUCCGGAAAUUUAAGAUUGCGAAUAAGCCCUCCCUCCAAAAAAGAGGGUGUAAAACAUUUAGAACCAAACCUUUCAUCAUGAGCAGUAAAACUCUCUCAAAGUUCAUAUCCUCAAAUCCCAUCAUCCAGUUUGUAAAAAGACUUCAAUCUGCAUGUGCUUUUUCGUUUCAAAAACGAAUAUAGUACAUAUAUACUAAUAUAAAAGUCUCGAAAAAGAAUCGGAUCGCCAAGAAUUCUUCUCAAAAAAUAAUUUUUUUCCACAACUACGAGAAAUUCAUAAACAACUAAUAUACAAUUAUAUACAAGUGCAUGUUAGAGGAUAGAUGUGCACAAAAGCAAAGUUCUUCACGCUUCAGAGGAUUGACUUUAAUAGAUUUUCAUACCUUUGCAUAGUACAAUCAUACCAAUAAAUUACAAAUAUGGAAAAUUGUAGUAUUCAAAGAGACUUCCUCAGCAAACUUGCAGAGGAUGCUGAUGCACACAACAUCAAGAAAAUUGAAAAUGCUUUUGUUCAUAAAGUGAACAAAGAUUAUGUGUUGAUUUCUGAAAUAAAGGAAGUUCCCGAAAAUUACUUGAAUAAUGAUGGGGAAAAAGAUGAAAAUUGCAAGUUUUUGUACUUAGUUGCCUUUCCUAAGCAUGAUUGUGUAUUUUGUUUUGACCAUGAAUUAGACCACCUCCCUUUCAUGAUGACUAUCGAGCUUAUUAGGCAAGCUGGAAUAGCGAUUGGGCAUGUAAUCCACAAAAUACCACUAAAAGGUUUUACAAACAUAAUGGAUUGCAUGAAUCUGAAUGUUCUAAAAUUUAUUGAGUUGGAUGUUCCUCUACUAAUCAUCAUCAAUGACAUAAUGUUGAAGAAUAAACCCAGCAGACAAGAAAGAAUGAUGCAUUUCUACUUAUAUCAAAAGAACUCACUAUGUGCUUCUGUUGACAUAAAUGCGUCCAUUAUGGAAAAAGAUAUAUAUCGACGAUUAAGAUUGAGCAGCCGGGCAGAGUUAAUACAAAACACUUCAUUGGACAAAGUCCCAACGACUAAUUUGCAAAUGCUUAACAAAAGAAUUUCAAUCAAAUAACAACGAUUUCUGACA .(((((......(((((.(((((((.(.((.....))).))))))).)))))....(((((.(((((((((((((((........(((((((..(((.......((..((......))..))...........((((((((..(((.....(((........)))....((((.((.......))))))..))).))))))))...........))))))))))........((((((((..(((((((..((((((.....))))))...)))))))...............((((((((..((((((.((....))....))))))...........(((((((((((...........(((((((((((((..(((((((((((((.((((((((((((((........)))))))))....((((..(((.((....(((((((.((..((.......(((((((....(((((.(((.((((((....(((..............)))...)))))).))).))))).....(((((.(((....))).)))))...(((((.......))))).)))))))........((((((((((...)))))))))).......))..)))))))))..........(((((.(..((((......))))..).)))))...)).)))..))))......)))))(((((.........))))).))))))))))))))))))).....)))))))...........(((((.((...........(((.....)))..(((((......((((((.(((((((((((......(((.......))).............))))))))))).....((((((((................)))))))).....)))))).((.(((((........))))).))......)))))...)).)))))...((((((((......(((.((((.((((..(((...))).)))).)))).))))))))))).....))))))))))).....)))).)))).))))))))....))))))))))..)))))......(((...((((...)))).............((....)).........)))..)))))...))))).............. (-257.24) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUCAGAAAUCGUUGUUAUUUGAUUGAAAUUCUUUUGUUAAGCAUUUGCAAAUUAGUCGUUGGGACUUUGUCCAAUGAAGUGUUUUGUAUUAACUCUGCCCGGCUGCUCAAUCUUAAUCGUCGAUAUAUAUCUUUUUCCAUAAUGGACGCAUUUAUGUCAACAGAAGCACAUAGUGAGUUCUUUUGAUAUAAGUAGAAAUGCAUCAUUCUUUCUUGUCUGCUGGGUUUAUUCUUCAACAUUAUGUCAUUGAUGAUGAUUAGUAGAGGAACAUCCAACUCAAUAAAUUUUAGAACAUUCAGAUUCAUGCAAUCCAUUAUGUUUGUAAAACCUUUUAGUGGUAUUUUGUGGAUUACAUGCCCAAUCGCUAUUCCAGCUUGCCUAAUAAGCUCGAUAGUCAUCAUGAAAGGGAGGUGGUCUAAUUCAUGGUCAAAACAAAAUACACAAUCAUGCUUAGGAAAGGCAACUAAGUACAAAAACUUGCAAUUUUCAUCUUUUUCCCCAUCAUUAUUCAAGUAAUUUUCGGGAACUUCCUUUAUUUCAGAAAUCAACACAUAAUCUUUGUUCACUUUAUGAACAAAAGCAUUUUCAAUUUUCUUGAUGUUGUGUGCAUCAGCAUCCUCUGCAAGUUUGCUGAGGAAGUCUCUUUGAAUACUACAAUUUUCCAUAUUUGUAAUUUAUUGGUAUGAUUGUACUAUGCAAAGGUAUGAAAAUCUAUUAAAGUCAAUCCUCUGAAGCGUGAAGAACUUUGCUUUUGUGCACAUCUAUCCUCUAACAUGCACUUGUAUAUAAUUGUAUAUUAGUUGUUUAUGAAUUUCUCGUAGUUGUGGAAAAAAAUUAUUUUUUGAGAAGAAUUCUUGGCGAUCCGAUUCUUUUUCGAGACUUUUAUAUUAGUAUAUAUGUACUAUAUUCGUUUUUGAAACGAAAAAGCACAUGCAGAUUGAAGUCUUUUUACAAACUGGAUGAUGGGAUUUGAGGAUAUGAACUUUGAGAGAGUUUUACUGCUCAUGAUGAAAGGUUUGGUUCUAAAUGUUUUACACCCUCUUUUUUGGAGGGAGGGCUUAUUCGCAAUCUUAAAUUUCCGGAGUUUGUUUAGUUCUCAGACAGAGUACACAUUCAAUAUUUUUAUUACCUUUGAAGCUUAUUUAGUCAUCAAAAGGAAUAAUCCAUUAUAACU ...........(((((.....(((((...((((((((.(((((...((((((....((((((((......)))))))).....))))))...((((((.((..(((((.((((...((((((((((((((...........(((((((.((..((((((((.((((.(((...)))..)))).)))))))).(((....)))...................)).))))))).........))))).)))))))))))))))))).)).......((((.(((((((((..(((..(((((((((.....(((((....((((((((((..........((((((((((((((((...(((..(((((((.(.((((((.....)))))).))))))......))..)))..)))))))..............))))))))).(((((.(((..((((.((....))..((((((((....((((..(((.((...(((((.(..(((((....)))))..).))))).........(((((((................(((((((.....))))))).((.(((((((......(((((((.....)))))))..(((((.(((....))).)))))....((((((.(((.(((((((..(((..............)))...))))))).))).))))))..)))))))))..............)))))))...)).)))..)))))))))))).......))))......(((....((((((....((((((((((.....(((((..(((((.((...((((.............(((..(((....)))..)))))))...))....)))))..)))))))))))))))))))))....((((((.....))))))...)))...))))))))......)))))))))))))))....))))))))).........(((((((..((......((((....(((((.(((((....))))).)))))..((((((.....)))))).))))......))..))))))).)))..))))))))).))))......)))))).............................))))).........)))))))).)))))....))))). (-276.73) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 931 140 91 231 182 NC_002950:PG1501|5end_transcriptional_regulator,_tetR_family_1579099..1579329_NEGATIVE 355 827 784 214 158 NC_002950:PG2013|5end_CRISPR-associated_protein_Cas2_2104489..2104719_NEGATIVE 342 1105 1061 207 165 NC_002950:PG2207|5end_hypothetical_protein_2320161..2320391_NEGATIVE 311 830 783 64 11 NC_002950:PG1992|5end_glucose-inhibited_division_protein_A_2081410..2081640_POSITIVE 310 797 758 108 52 NC_002950:PG0362|5end_hypothetical_protein_395301..395531_POSITIVE 308 690 643 118 69 NC_002950:PG0617|5end_hypothetical_protein_670059..670289_POSITIVE 308 579 536 127 83 NC_002950:PG0158|5end_competence_protein_F-related_protein_180175..180405_POSITIVE 306 828 788 180 125 NC_002950:PG1820|5end_cytochrome_c_nitrite_reductase,_catalytic_subunit_NrfA_1915889..1916119_NEGATIVE 305 828 783 198 147 NC_002950:PG0876|5end_tRNA_modification_GTPase_940806..941036_NEGATIVE 300 840 794 230 178 NC_002950:PG2205|5end_2-dehydropantoate_2-reductase_2316121..2316351_POSITIVE 299 819 774 123 59 NC_002950:PG1395|5end_cell_shape-determining_protein_MreC,_putative_1477669..1477899_NEGATIVE 299 820 773 143 91 NC_002950:PG0980|5end_hypothetical_protein_1040897..1041127_POSITIVE 298 820 778 51 6 NC_002950:PG0896|5end_beta-galactosidase_957249..957479_POSITIVE 297 823 787 111 69 NC_002950:PG0246|5end_hypothetical_protein_281241..281471_NEGATIVE 295 830 788 153 103 NC_002950:PG0374|5end_hypothetical_protein_405564..405794_NEGATIVE 291 449 402 195 146 NC_002950:PG0604|5end_4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase_660262..660492_POSITIVE 291 124 91 231 191 NC_002950:PG0123|5end_hypothetical_protein_144987..145217_POSITIVE 290 449 405 141 93 NC_002950:PG0022|5end_sulfate_permease_family_protein_26796..27026_NEGATIVE 289 820 773 207 161 NC_002950:PG0297|5end_hypothetical_protein_335518..335748_NEGATIVE 286 1119 1071 54 14 NC_002950:PG0196|5end_peptidase,_M16_family_232903..233133_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 321 820 774 151 105 NC_002950:PG0969|3end_S-adenosylmethionine:tRNA_ribosyltransferase-isomerase,_putative_1028201..1028351_NEGATIVE 311 1105 1068 36 1 NC_002950:PG2208|3end_hypothetical_protein_2320332..2320482_NEGATIVE 308 579 536 143 99 NC_002950:PG0157|3end_regulatory_protein_RecX_180271..180421_POSITIVE 299 819 774 85 21 NC_002950:PG1396|3end_cell_shape-determining_protein_MreB_1477707..1477857_NEGATIVE 284 820 773 149 104 NC_002950:PG0979|3end_hypothetical_protein_1040990..1041140_POSITIVE 284 147 110 64 28 NC_002950:PG0844|3end_hypothetical_protein_905461..905611_POSITIVE 284 806 761 123 67 NC_002950:PG0143|3end_hydrolase,_carbon-nitrogen_family_165747..165897_NEGATIVE 282 1097 1053 51 9 NC_002950:PG2178|3end_NADH:ubiquinone_oxidoreductase,_Na_translocating,_E_subunit_2286523..2286673_NEGATIVE 281 450 406 101 60 NC_002950:PG1841|3end_hypothetical_protein_1938313..1938463_POSITIVE 280 467 426 60 22 NC_002950:PG1533|3end_Toprim_domain_protein_1611713..1611863_NEGATIVE 279 567 521 151 100 NC_002950:PG1561|3end_dTDP-4-dehydrorhamnose_reductase_1640138..1640288_NEGATIVE 278 569 523 51 7 NC_002950:PG1776|3end_dnaJ_protein_1866702..1866852_POSITIVE 276 578 532 136 75 NC_002950:PG1633|3end_galactokinase_1714619..1714769_POSITIVE 276 840 791 143 92 NC_002950:PG1421|3end_ferredoxin,_4Fe-4S_1506874..1507024_NEGATIVE 276 460 413 116 71 NC_002950:PG0356|3end_hypothetical_protein_391473..391623_POSITIVE 274 735 692 123 79 NC_002950:PG0629|3end_inorganic_polyphosphate/ATP-NAD_kinase_682498..682648_NEGATIVE 269 568 523 122 69 NC_002950:PG2149|3end_hypothetical_protein_2256936..2257086_NEGATIVE 269 580 533 73 34 NC_002950:PG1598|3end_lipoprotein_signal_peptidase,_putative_1679170..1679320_POSITIVE 268 140 106 85 56 NC_002950:PG0626|3end_hypothetical_protein_680346..680496_POSITIVE 266 840 792 129 90 NC_002950:PG1159|3end_cobalamin_biosynthesis_protein_CbiB_1237957..1238107_NEGATIVE