Origin IGS: ctatttgacactacatcagaaagctcaagggagcacatcctatatccggcaaagatacgaaaaagagattatatcacaatgtctaatgtggatttatttcaggattgaagttgtatttttctgttatgtaatttagagtaaacacaaatgctgagcattatgtaatacagag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctctgtattacataatgctcagcatttgtgtttactctaaattacataacagaaaaatacaacttcaatcctgaaataaatccacattagacattgtgatataatctctttttcgtatctttgccggatataggatgtgctcccttgagctttctgatgtagtgtcaaatag Mask Tandem Repeat Region ================================================ ctatttgacactacatcagaaagctcaagggagcacatcctatatccggcaaagatacgaaaaagagattatatcacaatgtctaatgtggatttatttcaggattgaagttgtatttttctgttatgtaatttagagtaaacacaaatgctgagcattatgtaatacagag Find is-nt database================================================ Query_seq: PG1469:PG1470|PG1469:PG1470:type I restriction-modification system, M subunit, putative:hypothetical protein:<-->:1548089..1548260 172 ctatttgacactacatcagaaagctcaagggagcacatcctatatccggcaaagatacgaaaaagagattatatcacaatgtctaatgtggatttatttcaggattgaagttgtatttttctgttatgtaatttagagtaaacacaaatgctgagcattatgtaatacagag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1469:PG1470|PG1469:PG1470:type I restriction-modification system, M subunit, putative:hypothetical protein:<-->:1548089..1548260 172 ctatttgacactacatcagaaagctcaagggagcacatcctatatccggcaaagatacgaaaaagagattatatcacaatgtctaatgtggatttatttcaggattgaagttgtatttttctgttatgtaatttagagtaaacacaaatgctgagcattatgtaatacagag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1469:PG1470|PG1469:PG1470:type I restriction-modification system, M subunit, putative:hypothetical protein:<-->:1548089..1548260 172 ctatttgacactacatcagaaagctcaagggagcacatcctatatccggcaaagatacgaaaaagagattatatcacaatgtctaatgtggatttatttcaggattgaagttgtatttttctgttatgtaatttagagtaaacacaaatgctgagcattatgtaatacagag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctatttgacactacatcagaaagctcaagggagcacatcctatatccggcaaagatacgaaaaagagattatatcacaatgtctaatgtggatttatttcaggattgaagttgtatttttctgttatgtaatttagagtaaacacaaatgctgagcattatgtaatacagag Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 5 End: 133 .... M T L H Q K A Q G S T S Y I R Q R Y E K E I I S Q C L M W I Y F R I E V V F F C Y V I ....................................... ctatttgacactacatcagaaagctcaagggagcacatcctatatccggcaaagatacgaaaaagagattatatcacaatgtctaatgtggatttatttcaggattgaagttgtatttttctgttatgtaatttagagtaaacacaaatgctgagcattatgtaatacagag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctatttgacactacatcagaaagctcaagggagcacatcctatatccggcaaagatacgaaaaagagattatatcacaatgtctaatgtggatttatttcaggattgaagttgtatttttctgttatgtaatttagagtaaacacaaatgctgagcattatgtaatacagag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG1469:PG1470|PG1469:PG1470:type I restriction-modification system, M subunit, putative:hypothetical protein:<-->:1548089..1548260 172 ctatttgacactacatcagaaagctcaagggagcacatcctatatccggcaaagatacgaaaaagagattatatcacaatgtctaatgtggatttatttcaggattgaagttgtatttttctgttatgtaatttagagtaaacacaaatgctgagcattatgtaatacagag Intra-Species Hit: Count: 1 Min: 1 Max: 172 Len: 172 Subject: NC_002950_PG1469_PG1470|type I restriction-modification system, M subunit, putative:hypothetical protein|NEGATIVE:POSITIVE|[1548089,1548260]|172 HSP 1 e-value: 5.0E-93 bit: 341.0 Len: 172 Query Start:1 Query End:172 Subject Strand: POSITIVE Subject Start: 1 Subject End: 172 ctatttgacactacatcagaaagctcaagggagcacatcctatatccggcaaagatacgaaaaagagattatatcacaatgtctaatgtggatttatttcaggattgaagttgtatttttctgttatgtaatttagagtaaacacaaatgctgagcattatgtaatacagag ctatttgacactacatcagaaagctcaagggagcacatcctatatccggcaaagatacgaaaaagagattatatcacaatgtctaatgtggatttatttcaggattgaagttgtatttttctgttatgtaatttagagtaaacacaaatgctgagcattatgtaatacagag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUAUUUGACACUACAUCAGAAAGCUCAAGGGAGCACAUCCUAUAUCCGGCAAAGAUACGAAAAAGAGAUUAUAUCACAAUGUCUAAUGUGGAUUUAUUUCAGGAUUGAAGUUGUAUUUUUCUGUUAUGUAAUUUAGAGUAAACACAAAUGCUGAGCAUUAUGUAAUACAGAG .(((((.....(((((((((((((((....))))..((((..((((.......)))).......(((((....(((((........)))))....))))).))))............))))))..))))).....)))))...((((((((...))))).)))......... (-28.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUCUGUAUUACAUAAUGCUCAGCAUUUGUGUUUACUCUAAAUUACAUAACAGAAAAAUACAACUUCAAUCCUGAAAUAAAUCCACAUUAGACAUUGUGAUAUAAUCUCUUUUUCGUAUCUUUGCCGGAUAUAGGAUGUGCUCCCUUGAGCUUUCUGAUGUAGUGUCAAAUAG ....(((..(((((((((...))).)))))).))).....(((((((..(((((.............(((((..........((((........))))................((((((.....)))))))))))..((((....)))).))))))))))))......... (-31.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 957 60 11 151 102 NC_002950:PG1469|5end_type_I_restriction-modification_system,_M_subunit,_putative_1547998..1548228_NEGATIVE 323 67 38 117 86 NC_002950:PG2202|5end_Holliday_junction_resolvase-like_protein_2309933..2310163_NEGATIVE 300 55 11 177 141 NC_002950:PG0515|5end_hypothetical_protein_557958..558188_NEGATIVE 297 57 17 129 80 NC_002950:PG2030|5end_hypothetical_protein_2129747..2129977_POSITIVE 297 68 27 157 118 NC_002950:PG2026|5end_phosphoglycerate_mutase_family_protein_2124932..2125162_POSITIVE 291 60 15 45 3 NC_002950:PG0062|5end_TPR_domain_protein_73688..73918_POSITIVE 287 52 17 75 29 NC_002950:PG1277|5end_UDP-glucose-6_dehydrogenase,_putative_1353754..1353984_POSITIVE 283 50 5 143 97 NC_002950:PG1536|5end_cell_division_protein_FtsX,_putative_1616362..1616592_POSITIVE 276 70 22 219 164 NC_002950:PG0185|5end_ragA_protein_218329..218559_POSITIVE 275 68 23 86 43 NC_002950:PG1784|5end_hypothetical_protein_1872566..1872796_POSITIVE 273 70 25 205 153 NC_002950:PG2042|5end_thioredoxin_family_protein_2141109..2141339_NEGATIVE 273 47 3 81 34 NC_002950:PG2037|5end_hypothetical_protein_2137812..2138042_NEGATIVE 273 59 22 64 16 NC_002950:PG1964|5end_bacterial_sugar_transferase_2052910..2053140_POSITIVE 265 50 2 199 138 NC_002950:PG0227|5end_DNA_repair_protein_RadA_263978..264208_NEGATIVE 262 50 1 173 111 NC_002950:PG1162|5end_ATP:cob(I)alamin_adenosyltransferase,_putative_1242039..1242269_NEGATIVE 261 69 21 175 129 NC_002950:PG2177|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_F_2286504..2286734_NEGATIVE 259 59 11 66 4 NC_002950:PG2111|5end_thiS_protein_2221482..2221712_NEGATIVE 256 69 28 178 131 NC_002950:PG2187|5end_1,4-dihydroxy-2-naphthoate_octaprenyltransferase_2294643..2294873_NEGATIVE 255 59 14 227 183 NC_002950:PG2127|5end_hypothetical_protein_2233552..2233782_NEGATIVE 255 50 6 58 7 NC_002950:PG0485|5end_preprotein_translocase,_YajC_subunit_525324..525554_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 297 55 21 146 107 NC_002950:PG0308|3end_electron_transport_complex,_RnfABCDGE_type,_A_subunit_343827..343977_POSITIVE 277 65 29 66 30 NC_002950:PG0340|3end_hypothetical_protein_376436..376586_POSITIVE 275 68 23 98 55 NC_002950:PG1783|3end_glycosyl_transferase,_group_2_family_protein_1872658..1872808_POSITIVE 273 47 3 84 37 NC_002950:PG2038|3end_N-acetylmuramoyl-L-alanine_amidase,_putative_2137809..2137959_NEGATIVE 273 59 22 67 19 NC_002950:PG1963|3end_Sua5/YciO/YrdC/YwlC_family_protein_2052993..2053143_POSITIVE 273 60 14 123 71 NC_002950:PG0982|3end_TPR_domain_protein_1046642..1046792_POSITIVE 262 51 17 53 18 NC_002950:PG1786|3end_hypothetical_protein_1875322..1875472_POSITIVE 262 50 1 150 88 NC_002950:PG1163|3end_cobyrinic_acid_a,c-diamide_synthase_1242062..1242212_NEGATIVE 260 51 11 149 107 NC_002950:PG0030|3end_cytidine_deaminase_36402..36552_POSITIVE 259 53 22 144 113 NC_002950:PG0949|3end_hypothetical_protein_1009773..1009923_POSITIVE 257 129 82 78 15 NC_002950:PG0543|3end_transcriptional_regulator,_putative_595940..596090_POSITIVE 256 60 28 40 1 NC_002950:PG0061|3end_yngK_protein_73763..73913_POSITIVE 255 50 4 84 28 NC_002950:PG1950|3end_hypothetical_protein_2040840..2040990_POSITIVE 253 59 11 69 24 NC_002950:PG1220|3end_erythronate-4-phosphate_dehydrogenase_1298199..1298349_POSITIVE 252 66 30 42 5 NC_002950:PG0954|3end_TPR_domain_protein_1015005..1015155_POSITIVE 250 62 23 80 32 NC_002950:PG1900|3end_ribosome-associated_GTPase_1999799..1999949_NEGATIVE 250 66 23 61 6 NC_002950:PG1102|3end_hypothetical_protein_1170860..1171010_NEGATIVE 249 51 15 49 16 NC_002950:PG0395|3end_DNA-directed_RNA_polymerase_beta'_subunit_433305..433455_POSITIVE 247 51 16 102 55 NC_002950:PG1314|3end_chorismate_synthase_1394362..1394512_NEGATIVE 244 63 28 137 103 NC_002950:PG1169|3end_hypothetical_protein_1249083..1249233_NEGATIVE