Origin IGS: gtatatgtctatccatttattatcagtttaccaagttttctctgccagggaataagactacttactatttattactatgccatgctttcaattgctctgtaaaccataagtaagcacacgctccaatcctaccggatatatccgtacggccttatacaaatcgggcacaaatgttattacaaacgtaaggatgtggaagcactccttgaaaagtccaaccgaaaaatacagtgacaagtgagcggaatatactaacagcttttcagaggagacctatttatttccggcagggacgtctgcgaaccgctctatatcagctcccgtacatcccaggaaagatgctctacaaactttctgatattgaaacgttgttgcgagaaaactataagcccctatgagttaacaattactcataaaatgaaaaggtatcacaaggagacaaaagatagtatttgtgatcagctaagaaactatttaattattggcaaaatcaaacattaaatgcttcatggaaagaaatttctcatgcgaagaaacataacatgatataaaatctgcgaattccaaatccttagattgtaaaagttctgtttttcgattcaaagcacacaaacatatgttcaacattgatactatgttttcagtttttccttgaaggatatagccgatcaaacatccgttttgtggatatttacttgacaaataattatcaatgcctgtttctatatatcgcctatggagttggtttgccgaaatttttgtttttcgtccagctttgaaagaatcaatttcaattaaatttttggcttcagcaaaatactcccactgatttgtactccaaccagagaaacggaaatcaattcttgctgcacttttagcagcaatgccatcatttttatagagcctgtattcaggaataatatctatttgccattccatcctttttatattcgcatttatagatagtagtagttctttcgatatatcttcctcttccttgtcaatagaaatacttcctttagatttaacgacacaataaccatcgcacaataaagtcaaacaacgcctctccaatatctgcaatgtgcaatctatatactctgggttaaatcttccgc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gcggaagatttaacccagagtatatagattgcacattgcagatattggagaggcgttgtttgactttattgtgcgatggttattgtgtcgttaaatctaaaggaagtatttctattgacaaggaagaggaagatatatcgaaagaactactactatctataaatgcgaatataaaaaggatggaatggcaaatagatattattcctgaatacaggctctataaaaatgatggcattgctgctaaaagtgcagcaagaattgatttccgtttctctggttggagtacaaatcagtgggagtattttgctgaagccaaaaatttaattgaaattgattctttcaaagctggacgaaaaacaaaaatttcggcaaaccaactccataggcgatatatagaaacaggcattgataattatttgtcaagtaaatatccacaaaacggatgtttgatcggctatatccttcaaggaaaaactgaaaacatagtatcaatgttgaacatatgtttgtgtgctttgaatcgaaaaacagaacttttacaatctaaggatttggaattcgcagattttatatcatgttatgtttcttcgcatgagaaatttctttccatgaagcatttaatgtttgattttgccaataattaaatagtttcttagctgatcacaaatactatcttttgtctccttgtgataccttttcattttatgagtaattgttaactcataggggcttatagttttctcgcaacaacgtttcaatatcagaaagtttgtagagcatctttcctgggatgtacgggagctgatatagagcggttcgcagacgtccctgccggaaataaataggtctcctctgaaaagctgttagtatattccgctcacttgtcactgtatttttcggttggacttttcaaggagtgcttccacatccttacgtttgtaataacatttgtgcccgatttgtataaggccgtacggatatatccggtaggattggagcgtgtgcttacttatggtttacagagcaattgaaagcatggcatagtaataaatagtaagtagtcttattccctggcagagaaaacttggtaaactgataataaatggatagacatatac Mask Tandem Repeat Region ================================================ gtatatgtctatccatttattatcagtttaccaagttttctctgccagggaataagactacttactatttattactatgccatgctttcaattgctctgtaaaccataagtaagcacacgctccaatcctaccggatatatccgtacggccttatacaaatcgggcacaaatgttattacaaacgtaaggatgtggaagcactccttgaaaagtccaaccgaaaaatacagtgacaagtgagcggaatatactaacagcttttcagaggagacctatttatttccggcagggacgtctgcgaaccgctctatatcagctcccgtacatcccaggaaagatgctctacaaactttctgatattgaaacgttgttgcgagaaaactataagcccctatgagttaacaattactcataaaatgaaaaggtatcacaaggagacaaaagatagtatttgtgatcagctaagaaactatttaattattggcaaaatcaaacattaaatgcttcatggaaagaaatttctcatgcgaagaaacataacatgatataaaatctgcgaattccaaatccttagattgtaaaagttctgtttttcgattcaaagcacacaaacatatgttcaacattgatactatgttttcagtttttccttgaaggatatagccgatcaaacatccgttttgtggatatttacttgacaaataattatcaatgcctgtttctatatatcgcctatggagttggtttgccgaaatttttgtttttcgtccagctttgaaagaatcaatttcaattaaatttttggcttcagcaaaatactcccactgatttgtactccaaccagagaaacggaaatcaattcttgctgcacttttagcagcaatgccatcatttttatagagcctgtattcaggaataatatctatttgccattccatcctttttatattcgcatttatagatagtagtagttctttcgatatatcttcctcttccttgtcaatagaaatacttcctttagatttaacgacacaataaccatcgcacaataaagtcaaacaacgcctctccaatatctgcaatgtgcaatctatatactctgggttaaatcttccgc Find is-nt database================================================ Query_seq: PG1467:PG1469|PG1467:PG1469:methlytransferase, UbiE/COQ5 family:type I restriction-modification system, M subunit, putative:-><-:1543962..1545079 1118 gtatatgtctatccatttattatcagtttaccaagttttctctgccagggaataagactacttactatttattactatgccatgctttcaattgctctgtaaaccataagtaagcacacgctccaatcctaccggatatatccgtacggccttatacaaatcgggcacaaatgttattacaaacgtaaggatgtggaagcactccttgaaaagtccaaccgaaaaatacagtgacaagtgagcggaatatactaacagcttttcagaggagacctatttatttccggcagggacgtctgcgaaccgctctatatcagctcccgtacatcccaggaaagatgctctacaaactttctgatattgaaacgttgttgcgagaaaactataagcccctatgagttaacaattactcataaaatgaaaaggtatcacaaggagacaaaagatagtatttgtgatcagctaagaaactatttaattattggcaaaatcaaacattaaatgcttcatggaaagaaatttctcatgcgaagaaacataacatgatataaaatctgcgaattccaaatccttagattgtaaaagttctgtttttcgattcaaagcacacaaacatatgttcaacattgatactatgttttcagtttttccttgaaggatatagccgatcaaacatccgttttgtggatatttacttgacaaataattatcaatgcctgtttctatatatcgcctatggagttggtttgccgaaatttttgtttttcgtccagctttgaaagaatcaatttcaattaaatttttggcttcagcaaaatactcccactgatttgtactccaaccagagaaacggaaatcaattcttgctgcacttttagcagcaatgccatcatttttatagagcctgtattcaggaataatatctatttgccattccatcctttttatattcgcatttatagatagtagtagttctttcgatatatcttcctcttccttgtcaatagaaatacttcctttagatttaacgacacaataaccatcgcacaataaagtcaaacaacgcctctccaatatctgcaatgtgcaatctatatactctgggttaaatcttccgc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1467:PG1469|PG1467:PG1469:methlytransferase, UbiE/COQ5 family:type I restriction-modification system, M subunit, putative:-><-:1543962..1545079 1118 gtatatgtctatccatttattatcagtttaccaagttttctctgccagggaataagactacttactatttattactatgccatgctttcaattgctctgtaaaccataagtaagcacacgctccaatcctaccggatatatccgtacggccttatacaaatcgggcacaaatgttattacaaacgtaaggatgtggaagcactccttgaaaagtccaaccgaaaaatacagtgacaagtgagcggaatatactaacagcttttcagaggagacctatttatttccggcagggacgtctgcgaaccgctctatatcagctcccgtacatcccaggaaagatgctctacaaactttctgatattgaaacgttgttgcgagaaaactataagcccctatgagttaacaattactcataaaatgaaaaggtatcacaaggagacaaaagatagtatttgtgatcagctaagaaactatttaattattggcaaaatcaaacattaaatgcttcatggaaagaaatttctcatgcgaagaaacataacatgatataaaatctgcgaattccaaatccttagattgtaaaagttctgtttttcgattcaaagcacacaaacatatgttcaacattgatactatgttttcagtttttccttgaaggatatagccgatcaaacatccgttttgtggatatttacttgacaaataattatcaatgcctgtttctatatatcgcctatggagttggtttgccgaaatttttgtttttcgtccagctttgaaagaatcaatttcaattaaatttttggcttcagcaaaatactcccactgatttgtactccaaccagagaaacggaaatcaattcttgctgcacttttagcagcaatgccatcatttttatagagcctgtattcaggaataatatctatttgccattccatcctttttatattcgcatttatagatagtagtagttctttcgatatatcttcctcttccttgtcaatagaaatacttcctttagatttaacgacacaataaccatcgcacaataaagtcaaacaacgcctctccaatatctgcaatgtgcaatctatatactctgggttaaatcttccgc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1467:PG1469|PG1467:PG1469:methlytransferase, UbiE/COQ5 family:type I restriction-modification system, M subunit, putative:-><-:1543962..1545079 1118 gtatatgtctatccatttattatcagtttaccaagttttctctgccagggaataagactacttactatttattactatgccatgctttcaattgctctgtaaaccataagtaagcacacgctccaatcctaccggatatatccgtacggccttatacaaatcgggcacaaatgttattacaaacgtaaggatgtggaagcactccttgaaaagtccaaccgaaaaatacagtgacaagtgagcggaatatactaacagcttttcagaggagacctatttatttccggcagggacgtctgcgaaccgctctatatcagctcccgtacatcccaggaaagatgctctacaaactttctgatattgaaacgttgttgcgagaaaactataagcccctatgagttaacaattactcataaaatgaaaaggtatcacaaggagacaaaagatagtatttgtgatcagctaagaaactatttaattattggcaaaatcaaacattaaatgcttcatggaaagaaatttctcatgcgaagaaacataacatgatataaaatctgcgaattccaaatccttagattgtaaaagttctgtttttcgattcaaagcacacaaacatatgttcaacattgatactatgttttcagtttttccttgaaggatatagccgatcaaacatccgttttgtggatatttacttgacaaataattatcaatgcctgtttctatatatcgcctatggagttggtttgccgaaatttttgtttttcgtccagctttgaaagaatcaatttcaattaaatttttggcttcagcaaaatactcccactgatttgtactccaaccagagaaacggaaatcaattcttgctgcacttttagcagcaatgccatcatttttatagagcctgtattcaggaataatatctatttgccattccatcctttttatattcgcatttatagatagtagtagttctttcgatatatcttcctcttccttgtcaatagaaatacttcctttagatttaacgacacaataaccatcgcacaataaagtcaaacaacgcctctccaatatctgcaatgtgcaatctatatactctgggttaaatcttccgc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 484 Max: 993 Len: 510 Subject: UniRef90_A1ZMF5 Cluster: Hypothetical protein; n=1; Microscilla marina ATCC 23134|Rep: Hypothetical protein - Microscilla marina ATCC 23134 HSP 1 e-value: 6.0E-12 bit: 74.7 Len: 510 Query Start:484 Query End:993 Subject Strand: null Subject Start: 71 Subject End: 249 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E E D I S K E L L L S I N A N I K R M E W Q I D I I P E Y R L Y K N D G I A - - - - A K S A A R I D F R F S G W S - T N Q W E Y F A E A K N L I E I D S F K A G R K T K I S A N Q L H R R Y I E T G I D N Y L S S K Y P Q - N G C L I G Y I L Q G K T E N I V S M L N I C L - - C A L N R K T E L L Q S K D L E F A D F I S C Y V S S H E K F - - - L S M K H L M F D F A ............................................................................................................................. ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E E D I S E T L L E K M Q I Q P M A S L H K I S V Q R E Y Y L S R K E A T S K K T S A K E A R R I D F H F S N W T E E N E K K Y F A E A K N - V S C N D W A K K S G A K I Q A S N T V A R Y I A T G I K A L V D E K Y E K I Q G C M L A Y V V N G L P S D A V E K L N K K L Q K D S L P P K Y G L I E N K H - T I Q N Y E E C Y S S N N P N A P I N K T I K H I F L K F S ............................................................................................................................. gtatatgtctatccatttattatcagtttaccaagttttctctgccagggaataagactacttactatttattactatgccatgctttcaattgctctgtaaaccataagtaagcacacgctccaatcctaccggatatatccgtacggccttatacaaatcgggcacaaatgttattacaaacgtaaggatgtggaagcactccttgaaaagtccaaccgaaaaatacagtgacaagtgagcggaatatactaacagcttttcagaggagacctatttatttccggcagggacgtctgcgaaccgctctatatcagctcccgtacatcccaggaaagatgctctacaaactttctgatattgaaacgttgttgcgagaaaactataagcccctatgagttaacaattactcataaaatgaaaaggtatcacaaggagacaaaagatagtatttgtgatcagctaagaaactatttaattattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttccttgtcaatagaaatacttcctttagatttaacgacacaataaccatcgcacaataaagtcaaacaacgcctctccaatatctgcaatgtgcaatctatatactctgggttaaatcttccgc Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 3 End: 110 .. M C L S I Y Y Q F T K F S L P G N K T T Y Y L L L C H A F N C S V N H K ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 42 Strand: + Start: 106 End: 231 ......................................................................................................... M S K H T L Q S Y R I Y P Y G L I Q I G H K C Y Y K R K D V E A L L E K S N R K I Q ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 33 Strand: + Start: 140 End: 238 ........................................................................................................................................... M R T A L Y K S G T N V I T N V R M W K H S L K S P T E K Y S D K ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 55 Strand: + Start: 231 End: 395 ...................................................................................................................................................................................................................................... M T S E R N I L T A F Q R R P I Y F R Q G R L R T A L Y Q L P Y I P G K M L Y K L S D I E T L L R E N Y K P L ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 280 End: 498 ....................................................................................................................................................................................................................................................................................... M S G R D V C E P L Y I S S R T S Q E R C S T N F L I L K R C C E K T I S P Y E L T I T H K M K R Y H K E T K D S I C D ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 35 Strand: + Start: 548 End: 652 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M K S A N S K S L D C K S S V F R F K A H K H M F N I D T M F S V F P .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 576 End: 776 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M V K V L F F D S K H T N I C S T L I L C F Q F F L E G Y S R S N I R F V D I Y L T N N Y Q C L F L Y I A Y G V G L P K ...................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: + Start: 703 End: 795 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M I I N A C F Y I S P M E L V C R N F C F S S S F E R I N F N ................................................................................................................................................................................................................................................................................................................................... Protein_Len: 38 Strand: + Start: 713 End: 826 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M P V S I Y R L W S W F A E I F V F R P A L K E S I S I K F L A S A K Y S H .................................................................................................................................................................................................................................................................................................... Protein_Len: 55 Strand: + Start: 799 End: 963 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M F G F S K I L P L I C T P T R E T E I N S C C T F S S N A I I F I E P V F R N N I Y L P F H P F Y I R I Y R ........................................................................................................................................................... Protein_Len: 44 Strand: + Start: 905 End: 1036 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M Y S G I I S I C H S I L F I F A F I D S S S S F D I S S S S L S I E I L P L D L T T Q .................................................................................. Protein_Len: 42 Strand: - Start: 798 End: 923 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. I K P K L L I S G S I Q V G V L S V S I L E Q Q V K L L L A M M K I S G T N L F L M ................................................................................................................................................................................................... Protein_Len: 30 Strand: - Start: 510 End: 599 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. P F F N R M R L F M V H Y L I Q S N W I R L N Y F N Q K E M ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 478 End: 1095 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. I I D I Q W E M R K I N A N I S L L L E K S I D E E E K D I S I S G K S K V V C Y G D C L L T L C R R E L I Q L T C D M ....................... Protein_Len: 40 Strand: - Start: 277 End: 396 .................................................................................................................................................................................................................................................................................... K N G A P V D A F R E I D A G T C G L F S A R C V K Q Y Q F T T A L F S Y A G M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 32 Strand: - Start: 155 End: 250 .......................................................................................................................................................... V F R A C I N N C V Y P H P L V G Q F T W G F F V T V L S R F M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 56 Strand: - Start: 91 End: 258 .......................................................................................... N S Q L G Y T L V R E L G V P Y I R V A K Y L D P V F T I V F T L I H F C E K F L G V S F Y L S L H A S Y V L M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ gtatatgtctatccatttattatcagtttaccaagttttctctgccagggaataagactacttactatttattactatgccatgctttcaattgctctgtaaaccataagtaagcacacgctccaatcctaccggatatatccgtacggccttatacaaatcgggcacaaatgttattacaaacgtaaggatgtggaagcactccttgaaaagtccaaccgaaaaatacagtgacaagtgagcggaatatactaacagcttttcagaggagacctatttatttccggcagggacgtctgcgaaccgctctatatcagctcccgtacatcccaggaaagatgctctacaaactttctgatattgaaacgttgttgcgagaaaactataagcccctatgagttaacaattactcataaaatgaaaaggtatcacaaggagacaaaagatagtatttgtgatcagctaagaaactatttaattattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttccttgtcaatagaaatacttcctttagatttaacgacacaataaccatcgcacaataaagtcaaacaacgcctctccaatatctgcaatgtgcaatctatatactctgggttaaatcttccgc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gtatatgtctatccatttattatcagtttaccaagttttctctgccagggaataagactacttactatttattactatgccatgctttcaattgctctgtaaaccataagtaagcacacgctccaatcctaccggatatatccgtacggccttatacaaatcgggcacaaatgttattacaaacgtaaggatgtggaagcactccttgaaaagtccaaccgaaaaatacagtgacaagtgagcggaatatactaacagcttttcagaggagacctatttatttccggcagggacgtctgcgaaccgctctatatcagctcccgtacatcccaggaaagatgctctacaaactttctgatattgaaacgttgttgcgagaaaactataagcccctatgagttaacaattactcataaaatgaaaaggtatcacaaggagacaaaagatagtatttgtgatcagctaagaaactatttaattattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttccttgtcaatagaaatacttcctttagatttaacgacacaataaccatcgcacaataaagtcaaacaacgcctctccaatatctgcaatgtgcaatctatatactctgggttaaatcttccgc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG1467:PG1469|PG1467:PG1469:methlytransferase, UbiE/COQ5 family:type I restriction-modification system, M subunit, putative:-><-:1543962..1545079 1118 gtatatgtctatccatttattatcagtttaccaagttttctctgccagggaataagactacttactatttattactatgccatgctttcaattgctctgtaaaccataagtaagcacacgctccaatcctaccggatatatccgtacggccttatacaaatcgggcacaaatgttattacaaacgtaaggatgtggaagcactccttgaaaagtccaaccgaaaaatacagtgacaagtgagcggaatatactaacagcttttcagaggagacctatttatttccggcagggacgtctgcgaaccgctctatatcagctcccgtacatcccaggaaagatgctctacaaactttctgatattgaaacgttgttgcgagaaaactataagcccctatgagttaacaattactcataaaatgaaaaggtatcacaaggagacaaaagatagtatttgtgatcagctaagaaactatttaattattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttccttgtcaatagaaatacttcctttagatttaacgacacaataaccatcgcacaataaagtcaaacaacgcctctccaatatctgcaatgtgcaatctatatactctgggttaaatcttccgc Intra-Species Hit: Count: 1 Min: 1 Max: 1118 Len: 1118 Subject: NC_002950_PG1467_PG1469|methlytransferase, UbiE/COQ5 family:type I restriction-modification system, M subunit, putative|POSITIVE:NEGATIVE|[1543962,1545079]|1118 HSP 1 e-value: 0.0 bit: 957.0 Len: 483 Query Start:1 Query End:483 Subject Strand: POSITIVE Subject Start: 1 Subject End: 483 gtatatgtctatccatttattatcagtttaccaagttttctctgccagggaataagactacttactatttattactatgccatgctttcaattgctctgtaaaccataagtaagcacacgctccaatcctaccggatatatccgtacggccttatacaaatcgggcacaaatgttattacaaacgtaaggatgtggaagcactccttgaaaagtccaaccgaaaaatacagtgacaagtgagcggaatatactaacagcttttcagaggagacctatttatttccggcagggacgtctgcgaaccgctctatatcagctcccgtacatcccaggaaagatgctctacaaactttctgatattgaaacgttgttgcgagaaaactataagcccctatgagttaacaattactcataaaatgaaaaggtatcacaaggagacaaaagatagtatttgtgatcagctaagaaactatttaattatt........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... gtatatgtctatccatttattatcagtttaccaagttttctctgccagggaataagactacttactatttattactatgccatgctttcaattgctctgtaaaccataagtaagcacacgctccaatcctaccggatatatccgtacggccttatacaaatcgggcacaaatgttattacaaacgtaaggatgtggaagcactccttgaaaagtccaaccgaaaaatacagtgacaagtgagcggaatatactaacagcttttcagaggagacctatttatttccggcagggacgtctgcgaaccgctctatatcagctcccgtacatcccaggaaagatgctctacaaactttctgatattgaaacgttgttgcgagaaaactataagcccctatgagttaacaattactcataaaatgaaaaggtatcacaaggagacaaaagatagtatttgtgatcagctaagaaactatttaattatt........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 4.0E-64 bit: 248.0 Len: 125 Query Start:994 Query End:1118 Subject Strand: POSITIVE Subject Start: 994 Subject End: 1118 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttccttgtcaatagaaatacttcctttagatttaacgacacaataaccatcgcacaataaagtcaaacaacgcctctccaatatctgcaatgtgcaatctatatactctgggttaaatcttccgc .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttccttgtcaatagaaatacttcctttagatttaacgacacaataaccatcgcacaataaagtcaaacaacgcctctccaatatctgcaatgtgcaatctatatactctgggttaaatcttccgc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUAUAUGUCUAUCCAUUUAUUAUCAGUUUACCAAGUUUUCUCUGCCAGGGAAUAAGACUACUUACUAUUUAUUACUAUGCCAUGCUUUCAAUUGCUCUGUAAACCAUAAGUAAGCACACGCUCCAAUCCUACCGGAUAUAUCCGUACGGCCUUAUACAAAUCGGGCACAAAUGUUAUUACAAACGUAAGGAUGUGGAAGCACUCCUUGAAAAGUCCAACCGAAAAAUACAGUGACAAGUGAGCGGAAUAUACUAACAGCUUUUCAGAGGAGACCUAUUUAUUUCCGGCAGGGACGUCUGCGAACCGCUCUAUAUCAGCUCCCGUACAUCCCAGGAAAGAUGCUCUACAAACUUUCUGAUAUUGAAACGUUGUUGCGAGAAAACUAUAAGCCCCUAUGAGUUAACAAUUACUCAUAAAAUGAAAAGGUAUCACAAGGAGACAAAAGAUAGUAUUUGUGAUCAGCUAAGAAACUAUUUAAUUAUUGGCAAAAUCAAACAUUAAAUGCUUCAUGGAAAGAAAUUUCUCAUGCGAAGAAACAUAACAUGAUAUAAAAUCUGCGAAUUCCAAAUCCUUAGAUUGUAAAAGUUCUGUUUUUCGAUUCAAAGCACACAAACAUAUGUUCAACAUUGAUACUAUGUUUUCAGUUUUUCCUUGAAGGAUAUAGCCGAUCAAACAUCCGUUUUGUGGAUAUUUACUUGACAAAUAAUUAUCAAUGCCUGUUUCUAUAUAUCGCCUAUGGAGUUGGUUUGCCGAAAUUUUUGUUUUUCGUCCAGCUUUGAAAGAAUCAAUUUCAAUUAAAUUUUUGGCUUCAGCAAAAUACUCCCACUGAUUUGUACUCCAACCAGAGAAACGGAAAUCAAUUCUUGCUGCACUUUUAGCAGCAAUGCCAUCAUUUUUAUAGAGCCUGUAUUCAGGAAUAAUAUCUAUUUGCCAUUCCAUCCUUUUUAUAUUCGCAUUUAUAGAUAGUAGUAGUUCUUUCGAUAUAUCUUCCUCUUCCUUGUCAAUAGAAAUACUUCCUUUAGAUUUAACGACACAAUAACCAUCGCACAAUAAAGUCAAACAACGCCUCUCCAAUAUCUGCAAUGUGCAAUCUAUAUACUCUGGGUUAAAUCUUCCGC (((((((.................((((((..(((((((((.((.(((((((...(((((((.(((((((((....((((.............((((((((((......((((((.((.((((((.......((((....))))...(((..((((.....((((((....((((.(((((..(....)..))))).))))(((((((......((..............(..((((..((((((......................(((((........))))).(((((....)))))...)))))).(((((((.....(((((((........))))...))).......))))))).......))))..)..................(((((((........))))))).........)).....)))))))......((((((((((((((((.((((......((((((((..((((.....))))..))))))))(((((.(((((((...........(((((((.((.(((.........((((((.((........))..))))))......))).))))))))).............(((((((.(((((....))).)))))))))....))))))).)))))....)))).))))....(((((.....))))).........)))))).))))))..))))))......))))..)))..)))))))))))))).((((((....(((((((........)))))))...))))))...........((((...................((((((.(.(((......)))....).))))))....(((((((.......))))))).)))).....)))))))))).........(((((..((......))..)))))...............))))..)))))))))))))))).....((.......))...))))))).))..))))).))))....))))))...................(((((....((......................))....)))))....)))))))...(((........))).. (-235.91) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCGGAAGAUUUAACCCAGAGUAUAUAGAUUGCACAUUGCAGAUAUUGGAGAGGCGUUGUUUGACUUUAUUGUGCGAUGGUUAUUGUGUCGUUAAAUCUAAAGGAAGUAUUUCUAUUGACAAGGAAGAGGAAGAUAUAUCGAAAGAACUACUACUAUCUAUAAAUGCGAAUAUAAAAAGGAUGGAAUGGCAAAUAGAUAUUAUUCCUGAAUACAGGCUCUAUAAAAAUGAUGGCAUUGCUGCUAAAAGUGCAGCAAGAAUUGAUUUCCGUUUCUCUGGUUGGAGUACAAAUCAGUGGGAGUAUUUUGCUGAAGCCAAAAAUUUAAUUGAAAUUGAUUCUUUCAAAGCUGGACGAAAAACAAAAAUUUCGGCAAACCAACUCCAUAGGCGAUAUAUAGAAACAGGCAUUGAUAAUUAUUUGUCAAGUAAAUAUCCACAAAACGGAUGUUUGAUCGGCUAUAUCCUUCAAGGAAAAACUGAAAACAUAGUAUCAAUGUUGAACAUAUGUUUGUGUGCUUUGAAUCGAAAAACAGAACUUUUACAAUCUAAGGAUUUGGAAUUCGCAGAUUUUAUAUCAUGUUAUGUUUCUUCGCAUGAGAAAUUUCUUUCCAUGAAGCAUUUAAUGUUUGAUUUUGCCAAUAAUUAAAUAGUUUCUUAGCUGAUCACAAAUACUAUCUUUUGUCUCCUUGUGAUACCUUUUCAUUUUAUGAGUAAUUGUUAACUCAUAGGGGCUUAUAGUUUUCUCGCAACAACGUUUCAAUAUCAGAAAGUUUGUAGAGCAUCUUUCCUGGGAUGUACGGGAGCUGAUAUAGAGCGGUUCGCAGACGUCCCUGCCGGAAAUAAAUAGGUCUCCUCUGAAAAGCUGUUAGUAUAUUCCGCUCACUUGUCACUGUAUUUUUCGGUUGGACUUUUCAAGGAGUGCUUCCACAUCCUUACGUUUGUAAUAACAUUUGUGCCCGAUUUGUAUAAGGCCGUACGGAUAUAUCCGGUAGGAUUGGAGCGUGUGCUUACUUAUGGUUUACAGAGCAAUUGAAAGCAUGGCAUAGUAAUAAAUAGUAAGUAGUCUUAUUCCCUGGCAGAGAAAACUUGGUAAACUGAUAAUAAAUGGAUAGACAUAUAC .(((.(((((((((.((.((((.((..((((((((..(((((((.((......)).)))))).).....)))))))).)))))).))..))))))))).....((((.(((((.(((.((.((((.((((.......((....)).((((((((((.(((....(((((((((.(((((((((..((((((((((.(((((..((((....)))).((((((......((.((.(((((((.......)))))))..((((((((..((..((((....)))).)))))))))).(((((..((((((((((.....(((((.....)))))..(((.((((....)))).))).........))))))))))...)))))....)).)).)))))).........))))).))))))))))...((((((((.......))))))))(((((((((.....(((((((....((...(((((((...(((....)))...)))))))..)).)))))))..((((..((((.((((........)))).))))..(((.(((((..(((...(((...(((((((.......((((....)))).....)))))))...)))..))).))))).)))..........)))).))))))))).......))))))))).........(((((.(((((((...(((((((........)))))))(((((((((...(((((.((..................(((((..((.....)).)))))..(((((((..(.((((((........)))))).)..))))))).)).)))))...)))))))))...))))))).))))).)))))).)))..((((((((.(((...(((((((((........((((((((....))).)))))...(((((((...(((..(((..((...(((.(((..((...((((....))))...)).)))..)))..))..)))..)))..))))))).))))))))))))))))).))).))).))))).))))))))).)))).)).))))))))))))......))).......(((......))).... (-280.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 426 323 285 46 1 NC_002950:PG0444|5end_oligopeptide_transporter,_OPT_family_483256..483486_POSITIVE 421 153 114 66 31 NC_002950:PG1162|5end_ATP:cob(I)alamin_adenosyltransferase,_putative_1242039..1242269_NEGATIVE 393 319 283 52 21 NC_002950:PG1052|5end_transcriptional_regulator,_putative_1119690..1119920_POSITIVE 382 323 281 202 149 NC_002950:PG1655|5end_hypothetical_protein_1737095..1737325_POSITIVE 380 329 283 71 11 NC_002950:PG2069|5end_oxidoreductase,_short_chain_dehydrogenase/reductase_family_2164798..2165028_POSITIVE 368 299 256 228 183 NC_002950:PG2101|5end_hypothetical_protein_2211273..2211503_NEGATIVE 363 329 283 199 131 NC_002950:PG1381|5end_ABC_transporter,_permease_protein_1460622..1460852_POSITIVE 352 330 283 93 41 NC_002950:PG0123|5end_hypothetical_protein_144987..145217_POSITIVE 347 319 271 48 2 NC_002950:PG0219|5end_hypothetical_protein_255558..255788_NEGATIVE 346 330 283 93 26 NC_002950:PG1998|5end_polyprenyl_synthetase_2088029..2088259_NEGATIVE 345 307 265 198 146 NC_002950:PG0770|5end_hypothetical_protein_824575..824805_NEGATIVE 344 905 864 185 143 NC_002950:PG1817|5end_hypothetical_protein_1913035..1913265_NEGATIVE 343 306 282 66 43 NC_002950:PG0704|5end_phosphoglycerate_mutase_family_protein_758928..759158_POSITIVE 343 330 285 188 142 NC_002950:PG0293|5end_secretion_activator_protein,_putative_328671..328901_NEGATIVE 341 324 284 231 184 NC_002950:PG0305|5end_electron_transport_complex,_RnfABCDGE_type,_D_subunit_340906..341136_POSITIVE 338 324 284 83 40 NC_002950:PG0179|5end_hypothetical_protein_201783..202013_POSITIVE 336 310 265 71 5 NC_002950:PG0476|5end_yngK_protein_518762..518992_NEGATIVE 336 330 285 165 117 NC_002950:PG0164|5end_hypothetical_protein_187395..187625_NEGATIVE 335 320 271 196 152 NC_002950:PG0146|5end_hypothetical_protein_169115..169345_NEGATIVE 333 323 281 163 113 NC_002950:PG0955|5end_hypothetical_protein_1014832..1015062_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 421 153 114 43 8 NC_002950:PG1163|3end_cobyrinic_acid_a,c-diamide_synthase_1242062..1242212_NEGATIVE 401 330 281 128 81 NC_002950:PG2205|3end_2-dehydropantoate_2-reductase_2317145..2317295_POSITIVE 380 329 283 65 5 NC_002950:PG2068|3end_glycerol-3-phosphate_cytidylyltransferase_2164872..2165022_POSITIVE 373 323 284 61 21 NC_002950:PG0605|3end_hypothetical_protein_661165..661315_NEGATIVE 369 330 285 117 65 NC_002950:PG0021|3end_TIM-barrel_protein,_putative,_NifR3_family_24122..24272_NEGATIVE 368 299 256 77 32 NC_002950:PG2102|3end_immunoreactive_61_kDa_antigen_PG91_2211424..2211574_NEGATIVE 367 308 264 76 33 NC_002950:PG0443|3end_hemagglutinin-related_protein_483147..483297_POSITIVE 350 323 281 139 100 NC_002950:PG1625|3end_hypothetical_protein_1708718..1708868_POSITIVE 349 325 282 59 12 NC_002950:PG1786|3end_hypothetical_protein_1875322..1875472_POSITIVE 348 309 269 57 18 NC_002950:PG1133|3end_hypothetical_protein_1214103..1214253_POSITIVE 346 327 284 93 57 NC_002950:PG1391|3end_hypothetical_protein_1472961..1473111_POSITIVE 346 337 291 149 105 NC_002950:PG0003|3end_hypothetical_protein_1919..2069_NEGATIVE 344 326 281 46 2 NC_002950:PG0261|3end_ISPg3,_transposase_293862..294012_NEGATIVE 343 306 282 64 41 NC_002950:PG0703|3end_cobalamin_(5'-phosphate)_synthase,_putative_759006..759156_POSITIVE 336 310 265 81 15 NC_002950:PG0477|3end_pantoate--beta-alanine_ligase_518752..518902_NEGATIVE 333 322 289 66 28 NC_002950:PG0726|3end_lipoprotein,_putative_778003..778153_NEGATIVE 333 329 283 115 61 NC_002950:PG0333|3end_hypothetical_protein_368811..368961_POSITIVE 332 324 283 86 26 NC_002950:PG1638|3end_thioredoxin_family_protein_1718664..1718814_NEGATIVE 325 330 285 127 79 NC_002950:PG1389|3end_DNA-binding_protein,_histone-like_family_1470870..1471020_NEGATIVE 325 324 284 63 13 NC_002950:PG0085|3end_alpha-galactosidase_100556..100706_POSITIVE