Origin IGS: acatattatatcatattgctccttgcatggatgtaaaaatcagacttcccttttatttgaggaagcctgattaaccaatgcgattatcgctttttgaacttgccgcctttcagatcgctttccaccatacgcttattaagtttctcacggagttcatcaagaaaataggtgcggctatcatctttgcgacgtttcatgttcatatagacgttgtagctgttctttatcgccactccgaagatctccgagagagcgtctgccagttcctctacacctatttctccgttgttgatactgttgcaagtgtcgagagcataaaggagttcaactaaatctgttgctttgcctgtccaacagagtttcttagcaggtctgactggcaatgcaggaagaagtccctgcattcgcattttcagtaattcaagctccgtgtcaatgacaaagagcagttctgatacattttttcccctctccgtccattgaatctcccctttgcaaaggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaacctttgcaaaggggagattcaatggacggagaggggaaaaaatgtatcagaactgctctttgtcattgacacggagcttgaattactgaaaatgcgaatgcagggacttcttcctgcattgccagtcagacctgctaagaaactctgttggacaggcaaagcaacagatttagttgaactcctttatgctctcgacacttgcaacagtatcaacaacggagaaataggtgtagaggaactggcagacgctctctcggagatcttcggagtggcgataaagaacagctacaacgtctatatgaacatgaaacgtcgcaaagatgatagccgcacctattttcttgatgaactccgtgagaaacttaataagcgtatggtggaaagcgatctgaaaggcggcaagttcaaaaagcgataatcgcattggttaatcaggcttcctcaaataaaagggaagtctgatttttacatccatgcaaggagcaatatgatataatatgt Mask Tandem Repeat Region ================================================ acatattatatcatattgctccttgcatggatgtaaaaatcagacttcccttttatttgaggaagcctgattaaccaatgcgattatcgctttttgaacttgccgcctttcagatcgctttccaccatacgcttattaagtttctcacggagttcatcaagaaaataggtgcggctatcatctttgcgacgtttcatgttcatatagacgttgtagctgttctttatcgccactccgaagatctccgagagagcgtctgccagttcctctacacctatttctccgttgttgatactgttgcaagtgtcgagagcataaaggagttcaactaaatctgttgctttgcctgtccaacagagtttcttagcaggtctgactggcaatgcaggaagaagtccctgcattcgcattttcagtaattcaagctccgtgtcaatgacaaagagcagttctgatacattttttcccctctccgtccattgaatctcccctttgcaaaggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt Find is-nt database================================================ Query_seq: PG1444:PG1446|PG1444:PG1446:hypothetical protein:MATE efflux family protein:-><-:1529303..1529997 695 acatattatatcatattgctccttgcatggatgtaaaaatcagacttcccttttatttgaggaagcctgattaaccaatgcgattatcgctttttgaacttgccgcctttcagatcgctttccaccatacgcttattaagtttctcacggagttcatcaagaaaataggtgcggctatcatctttgcgacgtttcatgttcatatagacgttgtagctgttctttatcgccactccgaagatctccgagagagcgtctgccagttcctctacacctatttctccgttgttgatactgttgcaagtgtcgagagcataaaggagttcaactaaatctgttgctttgcctgtccaacagagtttcttagcaggtctgactggcaatgcaggaagaagtccctgcattcgcattttcagtaattcaagctccgtgtcaatgacaaagagcagttctgatacattttttcccctctccgtccattgaatctcccctttgcaaaggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1444:PG1446|PG1444:PG1446:hypothetical protein:MATE efflux family protein:-><-:1529303..1529997 695 acatattatatcatattgctccttgcatggatgtaaaaatcagacttcccttttatttgaggaagcctgattaaccaatgcgattatcgctttttgaacttgccgcctttcagatcgctttccaccatacgcttattaagtttctcacggagttcatcaagaaaataggtgcggctatcatctttgcgacgtttcatgttcatatagacgttgtagctgttctttatcgccactccgaagatctccgagagagcgtctgccagttcctctacacctatttctccgttgttgatactgttgcaagtgtcgagagcataaaggagttcaactaaatctgttgctttgcctgtccaacagagtttcttagcaggtctgactggcaatgcaggaagaagtccctgcattcgcattttcagtaattcaagctccgtgtcaatgacaaagagcagttctgatacattttttcccctctccgtccattgaatctcccctttgcaaaggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1444:PG1446|PG1444:PG1446:hypothetical protein:MATE efflux family protein:-><-:1529303..1529997 695 acatattatatcatattgctccttgcatggatgtaaaaatcagacttcccttttatttgaggaagcctgattaaccaatgcgattatcgctttttgaacttgccgcctttcagatcgctttccaccatacgcttattaagtttctcacggagttcatcaagaaaataggtgcggctatcatctttgcgacgtttcatgttcatatagacgttgtagctgttctttatcgccactccgaagatctccgagagagcgtctgccagttcctctacacctatttctccgttgttgatactgttgcaagtgtcgagagcataaaggagttcaactaaatctgttgctttgcctgtccaacagagtttcttagcaggtctgactggcaatgcaggaagaagtccctgcattcgcattttcagtaattcaagctccgtgtcaatgacaaagagcagttctgatacattttttcccctctccgtccattgaatctcccctttgcaaaggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 11 Min: 108 Max: 455 Len: 348 Subject: UniRef90_Q45784 Cluster: RteC protein; n=3; Bacteroides|Rep: RteC protein - Bacteroides thetaiotaomicron HSP 1 e-value: 2.0E-18 bit: 94.7 Len: 246 Query Start:114 Query End:359 Subject Strand: null Subject Start: 129 Subject End: 210 ................................................................................................................. L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D ................................................................................................................................................................................................................................................................................................................................................ ................................................................................................................. L Q W T G N A I D L V E L I Y G I D E M G C I N N G N M P L K Q L A P L L Y K I F G V E S K D C Y R F Y T D I K R R K N E S R T Y F L D R M Q E K L N E R M L R D D ................................................................................................................................................................................................................................................................................................................................................ Subject: UniRef90_Q8A5D2 Cluster: Tetracycline resistance element mobilization regulatory protein rteC; n=1; Bacteroides thetaiotaomicron|Rep: Tetracycline resistance element mobilization regulatory protein rteC - Bacteroides thetaiotaomicron HSP 1 e-value: 6.0E-12 bit: 73.6 Len: 246 Query Start:114 Query End:359 Subject Strand: null Subject Start: 117 Subject End: 198 ................................................................................................................. L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D ................................................................................................................................................................................................................................................................................................................................................ ................................................................................................................. V C W T A E V I C L M E L L Y A C H E L K M F N G G E V T L G R V V E Y V C H V L G V N V K N A S S Y Y A R M R R R K A G D R T Y F I D R L R E V L L K R M E D D D ................................................................................................................................................................................................................................................................................................................................................ Subject: UniRef90_A3UA55 Cluster: Putative regulatory protein; n=1; Croceibacter atlanticus HTCC2559|Rep: Putative regulatory protein - Croceibacter atlanticus HTCC2559 HSP 1 e-value: 2.0E-10 bit: 68.2 Len: 315 Query Start:108 Query End:422 Subject Strand: null Subject Start: 178 Subject End: 284 ........................................................................................................... E L L K M R M Q G L - L P A L P V R P A - - K K L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D L K ................................................................................................................................................................................................................................................................................. ........................................................................................................... E L D R L R K K E L N L S I E P D S P A I L N N L S W T A S K T D L V E I I Y A L Q A S G A I R N G Q A E I N K M A L V C E K L F D L E L G N F Y R T Y L E I K E R K N D - R T R F L T K L K R D L E Q K M K N D D E K ................................................................................................................................................................................................................................................................................. Subject: UniRef90_Q64WJ7 Cluster: Putative regulatory protein RteC homolog; n=1; Bacteroides fragilis|Rep: Putative regulatory protein RteC homolog - Bacteroides fragilis HSP 1 e-value: 7.0E-10 bit: 66.6 Len: 252 Query Start:114 Query End:365 Subject Strand: null Subject Start: 218 Subject End: 300 ................................................................................................................. K K L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D .......................................................................................................................................................................................................................................................................................................................................... ................................................................................................................. K V L R W T G T K R A L V E L I Y A L D A C G C L N K G T V D I K E I V A Y F E Y V F D I D L G D F Y H T Y M E L K A K T K D - R T G F L S T L K D R L L M R M R E Q D .......................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_A3XRA5 Cluster: Putative regulatory protein RteC-like protein; n=1; Leeuwenhoekiella blandensis MED217|Rep: Putative regulatory protein RteC-like protein - Leeuwenhoekiella blandensis MED217 HSP 1 e-value: 7.0E-10 bit: 66.6 Len: 252 Query Start:114 Query End:365 Subject Strand: null Subject Start: 195 Subject End: 277 ................................................................................................................. K K L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D .......................................................................................................................................................................................................................................................................................................................................... ................................................................................................................. K V L V W T G S K T A L V E L I Y A L Y S D Q V I N H G A L D L K Q L T S G F E D F F N I K L Q H P Y K T Y T E L K E R K - G S R T K F L N E L I L K L E Q K M L A E D .......................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q5LFW1 Cluster: Hypothetical protein; n=1; Bacteroides fragilis NCTC 9343|Rep: Hypothetical protein - Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) HSP 1 e-value: 1.0E-8 bit: 62.8 Len: 246 Query Start:108 Query End:353 Subject Strand: null Subject Start: 202 Subject End: 281 ........................................................................................................... W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D L K ...................................................................................................................................................................................................................................................................................................................................................... ........................................................................................................... F T G K K A Y L I E L G Y S L V S S G D I N N G N V E I K E M M N F L S T I F H I D L G D Y Y A S Y I A M K E R K D - - R T A Y L H H L I D S L I K R M N E D D M K ...................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q8A7U7 Cluster: Tetracycline resistance element mobilization regulatory protein rteC; n=1; Bacteroides thetaiotaomicron|Rep: Tetracycline resistance element mobilization regulatory protein rteC - Bacteroides thetaiotaomicron HSP 1 e-value: 1.0E-8 bit: 62.4 Len: 342 Query Start:114 Query End:455 Subject Strand: null Subject Start: 168 Subject End: 278 ................................................................................................................. S E L L F V I D T E L E L L K M R M Q G L L P A L P V R P A K K L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D ................................................................................................................................................................................................................................................ ................................................................................................................. S H L I E Y V S D E I D S L S D K L H - - L K F T S I V D S K L L Q W T D S K V A L V E F I Y A I Y A G K C F N N G N T S L K D I A F C C E I L F N I E I G D F Y R I F L E I R N R K - K S R T Q F L D K L K D K I Q K M M E E L D ................................................................................................................................................................................................................................................ Subject: UniRef90_A3UA76 Cluster: Putative regulatory protein; n=1; Croceibacter atlanticus HTCC2559|Rep: Putative regulatory protein - Croceibacter atlanticus HTCC2559 HSP 1 e-value: 1.0E-7 bit: 59.3 Len: 240 Query Start:114 Query End:353 Subject Strand: null Subject Start: 204 Subject End: 282 ................................................................................................................. W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D ...................................................................................................................................................................................................................................................................................................................................................... ................................................................................................................. W T S N K I D L V E L I Y A L Q T S R A I Q N G K I S I T T L A N V F G T V F N T D L S N I H R T Y V E L K S R K K D - R C K F M K Y L M V A L E N R V I T E E ...................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q1XQ17 Cluster: Putative regulatory protein RteC-like; n=1; Flavobacterium johnsoniae UW101|Rep: Putative regulatory protein RteC-like - Flavobacterium johnsoniae UW101 HSP 1 e-value: 1.0E-7 bit: 58.9 Len: 252 Query Start:108 Query End:359 Subject Strand: null Subject Start: 198 Subject End: 280 ........................................................................................................... L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D L K ................................................................................................................................................................................................................................................................................................................................................ ........................................................................................................... L V W T A S K T A L I E L I Y A L H T Q G V F G N G T I D I K V I A T Y F E Q T F N V D L G D F Y H T F L E L R N R K - T N R T K F I D T L K E G L L R R M D D Q E E K ................................................................................................................................................................................................................................................................................................................................................ Subject: UniRef90_A0M154 Cluster: Regulatory protein RteC; n=1; Gramella forsetii KT0803|Rep: Regulatory protein RteC - Gramella forsetii (strain KT0803) HSP 1 e-value: 1.0E-6 bit: 55.8 Len: 309 Query Start:114 Query End:422 Subject Strand: null Subject Start: 191 Subject End: 292 ................................................................................................................. E L L K M R M Q G L L P A L P V R P A K K L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D ................................................................................................................................................................................................................................................................................. ................................................................................................................. E A M E T L R P G K Q S G L M D K K H K V M L W S G S K T S L V E L I Y A L Y A N G D L N H G T V D I S T I T S S F E D F F N I K L E N I Y K T Y S E I K S R K - G T R A K Y L H N L I L R L E A K M N Q D D ................................................................................................................................................................................................................................................................................. Subject: UniRef90_A3XIY9 Cluster: Putative regulatory protein RteC-like protein; n=1; Leeuwenhoekiella blandensis MED217|Rep: Putative regulatory protein RteC-like protein - Leeuwenhoekiella blandensis MED217 HSP 1 e-value: 1.0E-6 bit: 55.8 Len: 264 Query Start:114 Query End:377 Subject Strand: null Subject Start: 191 Subject End: 277 ................................................................................................................. V R P A K K L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D .............................................................................................................................................................................................................................................................................................................................. ................................................................................................................. I K P M E K I D W P F S N T D Y V E L L Y A L C S K G L G K R N N Q G I M Q V S K K L E Q I F N I E P K D I Y K T F Q D I K N R K - K S R T L F L D D L S T S L L S E M N R S E .............................................................................................................................................................................................................................................................................................................................. acatattatatcatattgctccttgcatggatgtaaaaatcagacttcccttttatttgaggaagcctgattaaccaatgcgattatcgctttttgaacttgccgccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntacattttttcccctctccgtccattgaatctcccctttgcaaaggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 67 End: 165 .................................................................. M I N Q C D Y R F L N L P P F R S L S T I R L L S F S R S S S R K .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 33 Strand: + Start: 217 End: 315 ........................................................................................................................................................................................................................ M F F I A T P K I S E R A S A S S S T P I S P L L I L L Q V S R A ............................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 78 End: 329 ............................................................................. M R L S L F E L A A F Q I A F H H T L I K F L T E F I K K I G A A I I F A T F H V H I D V V A V L Y R H S E D L R E S V .............................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 46 Strand: + Start: 343 End: 480 ...................................................................................................................................................................................................................................................................................................................................................... M P V Q Q S F L A G L T G N A G R S P C I R I F S N S S S V S M T K S S S D T F F P L S V H ....................................................................................................................................................................................................................... Protein_Len: 39 Strand: + Start: 459 End: 575 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M F S P L R P L N L P F A K V A G K S V Y N G G S E T M R L N L F L H H I G Q ........................................................................................................................ Protein_Len: 60 Strand: - Start: 436 End: 693 ................................................................................................................................................................................................................................................................................................................................................................................................................................................... R T Y R L T R F L S V L D R E A D C L A I I K L I F N D S A K Q I Q G K T S C L S I I T K F D E T Q T L R L M I M D K M .. Protein_Len: 42 Strand: - Start: 416 End: 541 ............................................................................................................................................................................................................................................................................................................................................................................................................................... Y N L S R T L S L S C N Q Y M K E G R R G N F R G K A F T A P L L T Y L P P D S V M .......................................................................................................................................................... Protein_Len: 41 Strand: - Start: 181 End: 303 .................................................................................................................................................................................... R Q S T E H E Y L R Q L Q E K D G S R L D G L S R R G T G R C R N R R Q Q Y Q Q M ........................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: - Start: 87 End: 521 ...................................................................................... K G T W C L K K A P R V P L A P L L G Q M R M K L L E L E T D I V F L L E S V N K G R E T W Q I E G K C L N C A F A H M .............................................................................................................................................................................. acatattatatcatattgctccttgcatggatgtaaaaatcagacttcccttttatttgaggaagcctgattaaccaatgcgattatcgctttttgaacttgccgccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntacattttttcccctctccgtccattgaatctcccctttgcaaaggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acatattatatcatattgctccttgcatggatgtaaaaatcagacttcccttttatttgaggaagcctgattaaccaatgcgattatcgctttttgaacttgccgccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntacattttttcccctctccgtccattgaatctcccctttgcaaaggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 73 HP_score: -5.5 Tail_Score: -4.67353 Start: 40 End: 70 Full_Region: aatcgcattggttaa tcaggcttcct caaataaaa gggaagtctga tttttacatccatgc .......................................tcaggcttcctcaaataaaagggaagtctga................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 79 HP_score: -6.8 Tail_Score: -4.66833 Start: 80 End: 90 Full_Region: GCCTGATTAACCAAT GCGA TTA TCGC TTTTTGAACTTGCCG ...............................................................................GCGATTATCGC............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 56 HP_score: -3.4 Tail_Score: -3.92373 Start: 542 End: 554 Full_Region: AGGGTCCGAAACAAT GAGA CTAAA TCTC TTTCTGCATCACATA .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GAGACTAAATCTC............................................................................................................................................. Find igs database================================================ Query_seq: PG1444:PG1446|PG1444:PG1446:hypothetical protein:MATE efflux family protein:-><-:1529303..1529997 695 acatattatatcatattgctccttgcatggatgtaaaaatcagacttcccttttatttgaggaagcctgattaaccaatgcgattatcgctttttgaacttgccgccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntacattttttcccctctccgtccattgaatctcccctttgcaaaggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt Intra-Species Hit: Count: 2 Min: 1 Max: 695 Len: 695 Subject: NC_002950_PG1444_PG1446|hypothetical protein:MATE efflux family protein|POSITIVE:NEGATIVE|[1529303,1529997]|695 HSP 1 e-value: 1.0E-133 bit: 476.0 Len: 240 Query Start:456 Query End:695 Subject Strand: POSITIVE Subject Start: 456 Subject End: 695 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................tacattttttcccctctccgtccattgaatctcccctttgcaaaggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................tacattttttcccctctccgtccattgaatctcccctttgcaaaggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt HSP 2 e-value: 1.0E-53 bit: 212.0 Len: 107 Query Start:1 Query End:107 Subject Strand: POSITIVE Subject Start: 1 Subject End: 107 acatattatatcatattgctccttgcatggatgtaaaaatcagacttcccttttatttgaggaagcctgattaaccaatgcgattatcgctttttgaacttgccgcc............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ acatattatatcatattgctccttgcatggatgtaaaaatcagacttcccttttatttgaggaagcctgattaaccaatgcgattatcgctttttgaacttgccgcc............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Subject: NC_002950_PG0827_PG0829|MATE efflux family protein:hypothetical protein|POSITIVE:NEGATIVE|[885665,886359]|695 HSP 1 e-value: 1.0E-133 bit: 476.0 Len: 240 Query Start:456 Query End:695 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 240 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................tacattttttcccctctccgtccattgaatctcccctttgcaaaggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................tacattttttcccctctccgtccattgaatctcccctttgcaaaggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt HSP 2 e-value: 1.0E-53 bit: 212.0 Len: 107 Query Start:1 Query End:107 Subject Strand: NEGATIVE Subject Start: 589 Subject End: 695 acatattatatcatattgctccttgcatggatgtaaaaatcagacttcccttttatttgaggaagcctgattaaccaatgcgattatcgctttttgaacttgccgcc............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ acatattatatcatattgctccttgcatggatgtaaaaatcagacttcccttttatttgaggaagcctgattaaccaatgcgattatcgctttttgaacttgccgcc............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Inter-species Hit: Count: 2 Min: 456 Max: 695 Len: 240 Subject: pint_PI0395_PI0396|Na+-driven multidrug efflux pump:possible tetracycline resistance element mobilization regulatory protein, C-terminal fragment|POSITIVE:POSITIVE|[361845,362132]|288 HSP 1 e-value: 1.0E-130 bit: 468.0 Len: 240 Query Start:456 Query End:695 Subject Strand: NEGATIVE Subject Start: 4 Subject End: 243 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................tacattttttcccctctccgtccattgaatctcccctttgcaaaggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................tacattttttcccctctccgtccattgaatctcccctttgcaacggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt Subject: tfor_TF0922_TF0923|MATE efflux family protein:tetracycline resistance element mobilization regulatory protein|POSITIVE:POSITIVE|[961147,961395]|249 HSP 1 e-value: 6.0E-37 bit: 157.0 Len: 175 Query Start:518 Query End:692 Subject Strand: NEGATIVE Subject Start: 13 Subject End: 187 .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatctttta... .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gtatagcggaggatacgaaacaacgagacagtatcttcttctgtatcacatagagcaacaaccttcaatataaagttgtctgacgccttttgtatctgttcttttgtagaacaaaaagagagcattatcttaaagttttctgtttgagtaagtcttagcataatcatatctttta... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACAUUUUUUCCCCUCUCCGUCCAUUGAAUCUCCCCUUUGCAAAGGUUGCAGGCAAAAGCGUGUAUAACGGAGGGUCCGAAACAAUGAGACUAAAUCUCUUUCUGCAUCACAUAGGGCAAUAAUCUUCAGAAUAAAGUUGUCUGACGCUUUUUGUAUCUGUCCUUUUGUAGAGCAUAAAGAGAUUAUUGUCUUAAAGUCUUCAGUUUGAGUAAGUCUUAGCAUAAUCAUAUCUUUUAUGU ..((((.((((..((((((((.............((((....)))).((((.((....)).))))..))))))))...)))).)))).((((.(((((((((.(((.(((((.((((((.......(((((.........))))).((.....))...))))))..))).))))).))))))))).....((((((.(....).))))))..))))...((((((.........)))))) (-60.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACAUAAAAGAUAUGAUUAUGCUAAGACUUACUCAAACUGAAGACUUUAAGACAAUAAUCUCUUUAUGCUCUACAAAAGGACAGAUACAAAAAGCGUCAGACAACUUUAUUCUGAAGAUUAUUGCCCUAUGUGAUGCAGAAAGAGAUUUAGUCUCAUUGUUUCGGACCCUCCGUUAUACACGCUUUUGCCUGCAACCUUUGCAAAGGGGAGAUUCAAUGGACGGAGAGGGGAAAAAAUGUA ((((................................((((.(((..(.((((...(((((((((.(((..((((..(((...((((.........(((((.........)))))....))))..))).))))..))).)))))))))..)))).)..))))))).(((((((((........((((.((((((.....)))..))).)))).......))))))).)).......)))). (-53.88) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 364 60 18 46 1 NC_002950:PG2106|5end_hypothetical_protein_2214694..2214924_POSITIVE 337 75 31 81 34 NC_002950:PG0287|5end_hypothetical_protein_320457..320687_POSITIVE 333 70 21 70 26 NC_002950:PG0446|5end_thiF_protein_486599..486829_POSITIVE 331 77 35 83 45 NC_002950:PG1273|5end_hypothetical_protein_1351837..1352067_NEGATIVE 324 80 35 103 56 NC_002950:PG0289|5end_hypothetical_protein_322860..323090_POSITIVE 322 78 34 215 178 NC_002950:PG1934|5end_30S_ribosomal_protein_S19_2022345..2022575_NEGATIVE 320 60 11 108 44 NC_002950:PG1482|5end_conjugative_transposon_protein_TraF_1558779..1559009_NEGATIVE 313 90 44 166 113 NC_002950:PG1163|5end_cobyrinic_acid_a,c-diamide_synthase_1243381..1243611_NEGATIVE 312 59 11 231 176 NC_002950:PG2075|5end_hypothetical_protein_2176466..2176696_NEGATIVE 311 50 7 107 46 NC_002950:PG2116|5end_hypothetical_protein_2224635..2224865_POSITIVE 310 77 34 82 32 NC_002950:PG0155|5end_riboflavin_biosynthesis_protein_RibD_178853..179083_NEGATIVE 309 46 11 201 156 NC_002950:PG1285|5end_glucosamine-6-phosphate_deaminase_1362894..1363124_POSITIVE 308 77 34 195 146 NC_002950:PG1739|5end_hypothetical_protein_1825709..1825939_NEGATIVE 308 69 21 123 81 NC_002950:PG1549|5end_hypothetical_protein_1627947..1628177_NEGATIVE 307 120 71 210 162 NC_002950:PG0464|5end_adenylosuccinate_synthetase_508874..509104_NEGATIVE 305 80 34 41 1 NC_002950:PG0985|5end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_1046930..1047160_POSITIVE 304 80 34 223 180 NC_002950:PG1185|5end_hypothetical_protein_1267752..1267982_NEGATIVE 303 55 13 42 3 NC_002950:PG0163|5end_diphosphate--fructose-6-phosphate_1-phosphotransferase_186885..187115_NEGATIVE 301 89 47 198 159 NC_002950:PG0541|5end_hypothetical_protein_594757..594987_POSITIVE 301 56 15 154 110 NC_002950:PG0386|5end_site-specific_recombinase,_phage_integrase_family/ribosomal_subunit_interface_protein_419047..419277_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 782 210 161 60 11 NC_002950:PG0827|3end_MATE_efflux_family_protein_885604..885754_POSITIVE 384 60 12 70 26 NC_002950:PG2037|3end_hypothetical_protein_2137591..2137741_NEGATIVE 359 53 13 79 23 NC_002950:PG0656|3end_50S_ribosomal_protein_L34_706945..707095_NEGATIVE 349 80 32 57 4 NC_002950:PG1475|3end_conjugative_transposon_protein_TraN_1550810..1550960_NEGATIVE 337 60 13 105 57 NC_002950:PG1196|3end_hypothetical_protein_1275359..1275509_POSITIVE 321 79 31 100 40 NC_002950:PG1975|3end_hemagglutinin_protein_HagC_2064350..2064500_POSITIVE 321 80 36 89 49 NC_002950:PG1620|3end_carboxyl-terminal_protease-related_protein_1699415..1699565_NEGATIVE 320 60 11 104 40 NC_002950:PG1483|3end_conjugative_transposon_protein_TraE_1558783..1558933_NEGATIVE 315 78 32 146 100 NC_002950:PG0106|3end_glycosyl_transferase,_group_4_family_protein_129934..130084_POSITIVE 308 79 34 96 51 NC_002950:PG1116|3end_methylenetetrahydrofolate_dehydrogenase/methenyltetrahydrofolate_cyclohydrolase_1189901..1190051_POSITIVE 301 56 15 77 33 NC_002950:PG0385|3end_ribosomal_protein_S21_419050..419200_POSITIVE 298 74 35 145 101 NC_002950:PG0932|3end_DNA_polymerase_III,_delta_prime_subunit,_putative_989833..989983_POSITIVE 296 79 31 88 44 NC_002950:PG0607|3end_hypothetical_protein_662994..663144_POSITIVE 293 78 31 141 97 NC_002950:PG1846|3end_hypothetical_protein_1946254..1946404_POSITIVE 293 79 34 119 67 NC_002950:PG0453|3end_hypothetical_protein_496670..496820_POSITIVE 292 60 13 122 60 NC_002950:PG2214|3end_hypothetical_protein_2325301..2325451_NEGATIVE 292 46 1 56 23 NC_002950:PG0915|3end_hypothetical_protein_977057..977207_POSITIVE 290 70 21 129 83 NC_002950:PG2158|3end_hypothetical_protein_2264473..2264623_POSITIVE 288 59 11 64 22 NC_002950:PG1253|3end_DNA_ligase,_NAD-dependent_1329597..1329747_NEGATIVE 288 42 11 80 49 NC_002950:PG0507|3end_hypothetical_protein_548190..548340_POSITIVE