Origin IGS: acttacttaattttattgttatacaattcactgttcatttctttgccggtatatttacctgcaaagatgaatacatccactatctaagatatgcccgataatggggttgtaccagcagggctacatcctttcgttcataccctttttgtggagatgttcattgatgtccatcaatacaatcgttcgattgtatctgctttcacacatttccacgttcaagtgaaagaatcaaggacgtgttcttcgatataccccctcgcttatatcgctcatcgaatcgtgatatcatcgaagacacattcctccaccgacatattcgacggtgcaaatatatggctcggtgatgctgtaatcagcgtgatttactttagtgcgtacttgtggcttcgatttggatagttgtggcttcgtatgaggtaagggatttaggcacaacgcttagtaactttgcagtaagcgataagtcggtatttcatggctttgaaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcctgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaattatgtttagcacgttctatttccagaagagagaattacaaaccattaaaatccaaagta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tactttggattttaatggtttgtaattctctcttctggaaatagaacgtgctaaacataattgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaggagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgttttcaaagccatgaaataccgacttatcgcttactgcaaagttactaagcgttgtgcctaaatcccttacctcatacgaagccacaactatccaaatcgaagccacaagtacgcactaaagtaaatcacgctgattacagcatcaccgagccatatatttgcaccgtcgaatatgtcggtggaggaatgtgtcttcgatgatatcacgattcgatgagcgatataagcgagggggtatatcgaagaacacgtccttgattctttcacttgaacgtggaaatgtgtgaaagcagatacaatcgaacgattgtattgatggacatcaatgaacatctccacaaaaagggtatgaacgaaaggatgtagccctgctggtacaaccccattatcgggcatatcttagatagtggatgtattcatctttgcaggtaaatataccggcaaagaaatgaacagtgaattgtataacaataaaattaagtaagt Mask Tandem Repeat Region ================================================ acttacttaattttattgttatacaattcactgttcatttctttgccggtatatttacctgcaaagatgaatacatccactatctaagatatgcccgataatggggttgtaccagcagggctacatcctttcgttcataccctttttgtggagatgttcattgatgtccatcaatacaatcgttcgattgtatctgctttcacacatttccacgttcaagtgaaagaatcaaggacgtgttcttcgatataccccctcgcttatatcgctcatcgaatcgtgatatcatcgaagacacattcctccaccgacatattcgacggtgcaaatatatggctcggtgatgctgtaatcagcgtgatttactttagtgcgtacttgtggcttcgatttggatagttgtggcttcgtatgaggtaagggatttaggcacaacgcttagtaactttgcagtaagcgataagtcggtatttcatggctttgaaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcctgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaattatgtttagcacgttctatttccagaagagagaattacaaaccattaaaatccaaagta Find is-nt database================================================ Query_seq: PG1442:PG1444|PG1442:PG1444:hypothetical protein:hypothetical protein:<-->:1528121..1528885 765 acttacttaattttattgttatacaattcactgttcatttctttgccggtatatttacctgcaaagatgaatacatccactatctaagatatgcccgataatggggttgtaccagcagggctacatcctttcgttcataccctttttgtggagatgttcattgatgtccatcaatacaatcgttcgattgtatctgctttcacacatttccacgttcaagtgaaagaatcaaggacgtgttcttcgatataccccctcgcttatatcgctcatcgaatcgtgatatcatcgaagacacattcctccaccgacatattcgacggtgcaaatatatggctcggtgatgctgtaatcagcgtgatttactttagtgcgtacttgtggcttcgatttggatagttgtggcttcgtatgaggtaagggatttaggcacaacgcttagtaactttgcagtaagcgataagtcggtatttcatggctttgaaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcctgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaattatgtttagcacgttctatttccagaagagagaattacaaaccattaaaatccaaagta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1442:PG1444|PG1442:PG1444:hypothetical protein:hypothetical protein:<-->:1528121..1528885 765 acttacttaattttattgttatacaattcactgttcatttctttgccggtatatttacctgcaaagatgaatacatccactatctaagatatgcccgataatggggttgtaccagcagggctacatcctttcgttcataccctttttgtggagatgttcattgatgtccatcaatacaatcgttcgattgtatctgctttcacacatttccacgttcaagtgaaagaatcaaggacgtgttcttcgatataccccctcgcttatatcgctcatcgaatcgtgatatcatcgaagacacattcctccaccgacatattcgacggtgcaaatatatggctcggtgatgctgtaatcagcgtgatttactttagtgcgtacttgtggcttcgatttggatagttgtggcttcgtatgaggtaagggatttaggcacaacgcttagtaactttgcagtaagcgataagtcggtatttcatggctttgaaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcctgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaattatgtttagcacgttctatttccagaagagagaattacaaaccattaaaatccaaagta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1442:PG1444|PG1442:PG1444:hypothetical protein:hypothetical protein:<-->:1528121..1528885 765 acttacttaattttattgttatacaattcactgttcatttctttgccggtatatttacctgcaaagatgaatacatccactatctaagatatgcccgataatggggttgtaccagcagggctacatcctttcgttcataccctttttgtggagatgttcattgatgtccatcaatacaatcgttcgattgtatctgctttcacacatttccacgttcaagtgaaagaatcaaggacgtgttcttcgatataccccctcgcttatatcgctcatcgaatcgtgatatcatcgaagacacattcctccaccgacatattcgacggtgcaaatatatggctcggtgatgctgtaatcagcgtgatttactttagtgcgtacttgtggcttcgatttggatagttgtggcttcgtatgaggtaagggatttaggcacaacgcttagtaactttgcagtaagcgataagtcggtatttcatggctttgaaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcctgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaattatgtttagcacgttctatttccagaagagagaattacaaaccattaaaatccaaagta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acttacttaattttattgttatacaattcactgttcatttctttgccggtatatttacctgcaaagatgaatacatccactatctaagatatgcccgataatggggttgtaccagcagggctacatcctttcgttcataccctttttgtggagatgttcattgatgtccatcaatacaatcgttcgattgtatctgctttcacacatttccacgttcaagtgaaagaatcaaggacgtgttcttcgatataccccctcgcttatatcgctcatcgaatcgtgatatcatcgaagacacattcctccaccgacatattcgacggtgcaaatatatggctcggtgatgctgtaatcagcgtgatttactttagtgcgtacttgtggcttcgatttggatagttgtggcttcgtatgaggtaagggatttaggcacaacgcttagtaactttgcagtaagcgataagtcggtatttcatggctttgaaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcctgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaattatgtttagcacgttctatttccagaagagagaattacaaaccattaaaatccaaagta Predict ORF larger than 30AA ================================================ Protein_Len: 50 Strand: + Start: 71 End: 220 ...................................................................... M H P L S K I C P I M G L Y Q Q G Y I L S F I P F L W R C S L M S I N T I V R L Y L L S H I S T F K ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 56 Strand: + Start: 174 End: 341 ............................................................................................................................................................................. M Q S F D C I C F H T F P R S S E R I K D V F F D I P P R L Y R S S N R D I I E D T F L H R H I R R C K Y M A R ........................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 91 End: 396 .......................................................................................... M P D N G V V P A G L H P F V H T L F V E M F I D V H Q Y N R S I V S A F T H F H V Q V K E S R T C S S I Y P L A Y I A ................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 56 Strand: + Start: 381 End: 548 ............................................................................................................................................................................................................................................................................................................................................................................................ M A S I W I V V A S Y E V R D L G T T L S N F A V S D K S V F H G F E N I H F E R I T Y R S S E R I Y L R Q C K ......................................................................................................................................................................................................................... Protein_Len: 45 Strand: + Start: 511 End: 645 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M V L R N E F I C V S A N S K V C L A L P E I I S V T Q N P C S C R G Q N T S E V G V L G ........................................................................................................................ Protein_Len: 45 Strand: + Start: 558 End: 692 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M S C V T R N Y F G N S K P L L L Q R A E H L R S R C S W I E V F V V L I G Y F I V N A L ......................................................................... Protein_Len: 36 Strand: - Start: 442 End: 549 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................... Y S Q L L R Y T P I E H S Q F C V N R S F L M D N K P V F K N A D T C M ........................................................................................................................................................................................................................ Protein_Len: 51 Strand: - Start: 429 End: 581 ............................................................................................................................................................................................................................................................................................................................................................................................................................................ A C R K T V K C Y A I L R Y K M A K F V Y M E L S Y C I T R R F S N I Q T L A F L L T H R A N G S I M ........................................................................................................................................................................................ Protein_Len: 49 Strand: - Start: 289 End: 435 ................................................................................................................................................................................................................................................................................................ R L C M G G G V Y E V T C I Y P E T I S Y D A H N V K T R V Q P K S K S L Q P K T H P L P N L C M .......................................................................................................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: - Start: 273 End: 362 ................................................................................................................................................................................................................................................................................ R I T I D D F V C E E V S M N S P A F I H S P S A T I L T M ................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 44 Strand: - Start: 203 End: 334 .......................................................................................................................................................................................................... V N G R E L S L I L S T N K S I G G R K Y R E D F R S I M S S V N R W R C I R R H L Y M ............................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 32 Strand: - Start: 124 End: 219 ........................................................................................................................... M R E N M G K K H L H E N I D M L V I T R N Y R S E C M E V N M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. acttacttaattttattgttatacaattcactgttcatttctttgccggtatatttacctgcaaagatgaatacatccactatctaagatatgcccgataatggggttgtaccagcagggctacatcctttcgttcataccctttttgtggagatgttcattgatgtccatcaatacaatcgttcgattgtatctgctttcacacatttccacgttcaagtgaaagaatcaaggacgtgttcttcgatataccccctcgcttatatcgctcatcgaatcgtgatatcatcgaagacacattcctccaccgacatattcgacggtgcaaatatatggctcggtgatgctgtaatcagcgtgatttactttagtgcgtacttgtggcttcgatttggatagttgtggcttcgtatgaggtaagggatttaggcacaacgcttagtaactttgcagtaagcgataagtcggtatttcatggctttgaaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcctgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaattatgtttagcacgttctatttccagaagagagaattacaaaccattaaaatccaaagta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 83 Start: 26 End: 55 .........................ATTCACTGTTCATTTCTTTGCCGGTATATT...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acttacttaattttattgttatacaattcactgttcatttctttgccggtatatttacctgcaaagatgaatacatccactatctaagatatgcccgataatggggttgtaccagcagggctacatcctttcgttcataccctttttgtggagatgttcattgatgtccatcaatacaatcgttcgattgtatctgctttcacacatttccacgttcaagtgaaagaatcaaggacgtgttcttcgatataccccctcgcttatatcgctcatcgaatcgtgatatcatcgaagacacattcctccaccgacatattcgacggtgcaaatatatggctcggtgatgctgtaatcagcgtgatttactttagtgcgtacttgtggcttcgatttggatagttgtggcttcgtatgaggtaagggatttaggcacaacgcttagtaactttgcagtaagcgataagtcggtatttcatggctttgaaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcctgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaattatgtttagcacgttctatttccagaagagagaattacaaaccattaaaatccaaagta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 70 HP_score: -5.4 Tail_Score: -4.46514 Start: 518 End: 528 Full_Region: AATAACATATCGTTC TTCG GAA CGAA TTTATTTGCGTCAGT .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTCGGAACGAA............................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 52 HP_score: -4.7 Tail_Score: -3.38046 Start: 551 End: 566 Full_Region: ataatttcgggtaac gcaag acatac cttgc tatttgcactgacgc ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gcaagacataccttgc....................................................................................................................................................................................................... Find igs database================================================ Query_seq: PG1442:PG1444|PG1442:PG1444:hypothetical protein:hypothetical protein:<-->:1528121..1528885 765 acttacttaattttattgttatacaattcactgttcatttctttgccggtatatttacctgcaaagatgaatacatccactatctaagatatgcccgataatggggttgtaccagcagggctacatcctttcgttcataccctttttgtggagatgttcattgatgtccatcaatacaatcgttcgattgtatctgctttcacacatttccacgttcaagtgaaagaatcaaggacgtgttcttcgatataccccctcgcttatatcgctcatcgaatcgtgatatcatcgaagacacattcctccaccgacatattcgacggtgcaaatatatggctcggtgatgctgtaatcagcgtgatttactttagtgcgtacttgtggcttcgatttggatagttgtggcttcgtatgaggtaagggatttaggcacaacgcttagtaactttgcagtaagcgataagtcggtatttcatggctttgaaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcctgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaattatgtttagcacgttctatttccagaagagagaattacaaaccattaaaatccaaagta Intra-Species Hit: Count: 2 Min: 1 Max: 765 Len: 765 Subject: NC_002950_PG1442_PG1444|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[1528121,1528885]|765 HSP 1 e-value: 0.0 bit: 1516.0 Len: 765 Query Start:1 Query End:765 Subject Strand: POSITIVE Subject Start: 1 Subject End: 765 acttacttaattttattgttatacaattcactgttcatttctttgccggtatatttacctgcaaagatgaatacatccactatctaagatatgcccgataatggggttgtaccagcagggctacatcctttcgttcataccctttttgtggagatgttcattgatgtccatcaatacaatcgttcgattgtatctgctttcacacatttccacgttcaagtgaaagaatcaaggacgtgttcttcgatataccccctcgcttatatcgctcatcgaatcgtgatatcatcgaagacacattcctccaccgacatattcgacggtgcaaatatatggctcggtgatgctgtaatcagcgtgatttactttagtgcgtacttgtggcttcgatttggatagttgtggcttcgtatgaggtaagggatttaggcacaacgcttagtaactttgcagtaagcgataagtcggtatttcatggctttgaaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcctgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaattatgtttagcacgttctatttccagaagagagaattacaaaccattaaaatccaaagta acttacttaattttattgttatacaattcactgttcatttctttgccggtatatttacctgcaaagatgaatacatccactatctaagatatgcccgataatggggttgtaccagcagggctacatcctttcgttcataccctttttgtggagatgttcattgatgtccatcaatacaatcgttcgattgtatctgctttcacacatttccacgttcaagtgaaagaatcaaggacgtgttcttcgatataccccctcgcttatatcgctcatcgaatcgtgatatcatcgaagacacattcctccaccgacatattcgacggtgcaaatatatggctcggtgatgctgtaatcagcgtgatttactttagtgcgtacttgtggcttcgatttggatagttgtggcttcgtatgaggtaagggatttaggcacaacgcttagtaactttgcagtaagcgataagtcggtatttcatggctttgaaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcctgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaattatgtttagcacgttctatttccagaagagagaattacaaaccattaaaatccaaagta Subject: NC_002950_PG0829_PG0831|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[886837,887542]|706 HSP 1 e-value: 1.0E-140 bit: 500.0 Len: 264 Query Start:442 Query End:705 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 264 .........................................................................................................................................................................................................................................................................................................................................................................................................................................................gtaactttgcagtaagcgataagtcggtatttcatggctttgaaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcctgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaa............................................................ .........................................................................................................................................................................................................................................................................................................................................................................................................................................................gtaactttgcagtaagcgataagtcggtatttcatggcttgggaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcttgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaa............................................................ HSP 2 e-value: 1.0E-136 bit: 486.0 Len: 401 Query Start:11 Query End:411 Subject Strand: NEGATIVE Subject Start: 295 Subject End: 695 ..........ttttattgttatacaattcactgttcatttctttgccggtatatttacctgcaaagatgaatacatccactatctaagatatgcccgataatggggttgtaccagcagggctacatcctttcgttcataccctttttgtggagatgttcattgatgtccatcaatacaatcgttcgattgtatctgctttcacacatttccacgttcaagtgaaagaatcaaggacgtgttcttcgatataccccctcgcttatatcgctcatcgaatcgtgatatcatcgaagacacattcctccaccgacatattcgacggtgcaaatatatggctcggtgatgctgtaatcagcgtgatttactttagtgcgtacttgtggcttcgatttggatagttgtggcttcgt.................................................................................................................................................................................................................................................................................................................................................................. ..........ttttattattacacaattcattgttcatttcgttgccagtatatttaccgatgaagatgaatacatccgctatcgaagatatgcccaataatcggcttgtaccagtagggctacatcctttcattcataccctttctgtggagatgttcattagtgtccatcaatacaatcgttcgattgtatctgccttcgcacattcccacgttcgagtgaaagaatcaaggacatgttctttgacatacaccctcgcctatatcgctcatcgaaacgtgatgttatcgaagacacataccatcaccgacatattcgacagtgcaaatatatggttcggtgatgctgtaatcagcgtgatttcctttagtgcgtacttgtggcatcgatttggataattgtggcttcgt.................................................................................................................................................................................................................................................................................................................................................................. Inter-species Hit: Count: 3 Min: 1 Max: 765 Len: 765 Subject: pint_PI0397_PI0398|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[362960,363517]|558 HSP 1 e-value: 0.0 bit: 1074.0 Len: 558 Query Start:208 Query End:765 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 558 ...............................................................................................................................................................................................................ttccacgttcaagtgaaagaatcaaggacgtgttcttcgatataccccctcgcttatatcgctcatcgaatcgtgatatcatcgaagacacattcctccaccgacatattcgacggtgcaaatatatggctcggtgatgctgtaatcagcgtgatttactttagtgcgtacttgtggcttcgatttggatagttgtggcttcgtatgaggtaagggatttaggcacaacgcttagtaactttgcagtaagcgataagtcggtatttcatggctttgaaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcctgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaattatgtttagcacgttctatttccagaagagagaattacaaaccattaaaatccaaagta ...............................................................................................................................................................................................................ttccacgttcaagtgaaagaatcaaggacgtgttctttgatataccccctcgcttatatcgctcatcgaatcgtgatatcatcgaagacacattcctccaccgacatattcgacggtgcaaatatatggctcggtgatgctgtaatcagcgtgatttactttagtgcgtacttgtggcttcgatttggatagttgtggcttcgtatgaggtaagggatttaggcacaacacttagtaactttgcagtaagcgataagtcggtatttcatggctttgaaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcttgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaattgtgtttagcacgttctatttccagaagagagaattacaaaccattaaaatccaaagta Subject: pint_PI0398_PI0399|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[363602,363724]|123 HSP 1 e-value: 4.0E-63 bit: 244.0 Len: 123 Query Start:1 Query End:123 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 123 acttacttaattttattgttatacaattcactgttcatttctttgccggtatatttacctgcaaagatgaatacatccactatctaagatatgcccgataatggggttgtaccagcagggcta.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. acttacttaattttattgttatacaattcactgttcatttctttgccggtatatttacctgcaaagatgaatacatccactatctaagatatgcccgataatggggttgtaccagcagggcta.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Subject: pint_PI0246_PI0247|conserved hypothetical protein:conjugative transposon protein, TraA|POSITIVE:POSITIVE|[244660,245512]|853 HSP 1 e-value: 2.0E-21 bit: 105.0 Len: 81 Query Start:372 Query End:452 Subject Strand: NEGATIVE Subject Start: 315 Subject End: 395 ...................................................................................................................................................................................................................................................................................................................................................................................tgcgtacttgtggcttcgatttggatagttgtggcttcgtatgaggtaagggatttaggcacaacgcttagtaactttgca......................................................................................................................................................................................................................................................................................................................... ...................................................................................................................................................................................................................................................................................................................................................................................tgcgtacttgtggcttcgatttgcgtatttgtggctccgtaagaggtaagtgctttaggcacaacgcttagtaactttgca......................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-16 bit: 89.7 Len: 89 Query Start:528 Query End:616 Subject Strand: NEGATIVE Subject Start: 149 Subject End: 237 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................atttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcctgcagagggc..................................................................................................................................................... ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................atttatttgcgccggagcaaatagcaagatatgtcctgcgttactcgaaataaattcggtaactcagaactcttgctcttgcagagggc..................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACUUACUUAAUUUUAUUGUUAUACAAUUCACUGUUCAUUUCUUUGCCGGUAUAUUUACCUGCAAAGAUGAAUACAUCCACUAUCUAAGAUAUGCCCGAUAAUGGGGUUGUACCAGCAGGGCUACAUCCUUUCGUUCAUACCCUUUUUGUGGAGAUGUUCAUUGAUGUCCAUCAAUACAAUCGUUCGAUUGUAUCUGCUUUCACACAUUUCCACGUUCAAGUGAAAGAAUCAAGGACGUGUUCUUCGAUAUACCCCCUCGCUUAUAUCGCUCAUCGAAUCGUGAUAUCAUCGAAGACACAUUCCUCCACCGACAUAUUCGACGGUGCAAAUAUAUGGCUCGGUGAUGCUGUAAUCAGCGUGAUUUACUUUAGUGCGUACUUGUGGCUUCGAUUUGGAUAGUUGUGGCUUCGUAUGAGGUAAGGGAUUUAGGCACAACGCUUAGUAACUUUGCAGUAAGCGAUAAGUCGGUAUUUCAUGGCUUUGAAAACAUACAUUUCGAGAGAAUAACAUAUCGUUCUUCGGAACGAAUUUAUUUGCGUCAGUGCAAAUAGCAAGGUAUGUCUUGCGUUACCCGAAAUUAUUUCGGUAACUCAAAACCCUUGCUCCUGCAGAGGGCAGAACACCUCCGAAGUCGGUGUUCUUGGAUAGAAGUUUUCGUUGUCUUGAUUGGCUAUUUUAUCGUGAACGCUCUCUAAUGGAUUUCAAUUAUGUUUAGCACGUUCUAUUUCCAGAAGAGAGAAUUACAAACCAUUAAAAUCCAAAGUA ...(((((.((((((.((........(((((.(((((..(((((((.((((....)))).))))))))))))............(((((((.(((((((...(((((.(((...(.(((((.((.(((((((.(((((........(((((((((((...(((((....)))))(((((((....)))))))...........))))))))))).....))))).))...))))).))))))).)..)))))))).((((((((....((((((((((((..((((((((((((.((((......(((((.(......).)))))......(((((((.((((((((((....))))))....))))..))).))))..)))).)))))))...))).))..))).)))).)))))((((((...((((((.....))))))...)))))).))))))))...)))).....(((.(((..(((((((......(((....))).......((((((....)))))).(((((((..(((..((((...(((((((...((.(((.((((((.(((.....))))))))).))).)))))))))..))))...))).(((((((.........)))))))..))))))).)))))))..))).)))..))))))))))..)))))..((((((..((((........((((.....))))......))))..))))))..........)).))))))...))))) (-226.26) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACUUUGGAUUUUAAUGGUUUGUAAUUCUCUCUUCUGGAAAUAGAACGUGCUAAACAUAAUUGAAAUCCAUUAGAGAGCGUUCACGAUAAAAUAGCCAAUCAAGACAACGAAAACUUCUAUCCAAGAACACCGACUUCGGAGGUGUUCUGCCCUCUGCAGGAGCAAGGGUUUUGAGUUACCGAAAUAAUUUCGGGUAACGCAAGACAUACCUUGCUAUUUGCACUGACGCAAAUAAAUUCGUUCCGAAGAACGAUAUGUUAUUCUCUCGAAAUGUAUGUUUUCAAAGCCAUGAAAUACCGACUUAUCGCUUACUGCAAAGUUACUAAGCGUUGUGCCUAAAUCCCUUACCUCAUACGAAGCCACAACUAUCCAAAUCGAAGCCACAAGUACGCACUAAAGUAAAUCACGCUGAUUACAGCAUCACCGAGCCAUAUAUUUGCACCGUCGAAUAUGUCGGUGGAGGAAUGUGUCUUCGAUGAUAUCACGAUUCGAUGAGCGAUAUAAGCGAGGGGGUAUAUCGAAGAACACGUCCUUGAUUCUUUCACUUGAACGUGGAAAUGUGUGAAAGCAGAUACAAUCGAACGAUUGUAUUGAUGGACAUCAAUGAACAUCUCCACAAAAAGGGUAUGAACGAAAGGAUGUAGCCCUGCUGGUACAACCCCAUUAUCGGGCAUAUCUUAGAUAGUGGAUGUAUUCAUCUUUGCAGGUAAAUAUACCGGCAAAGAAAUGAACAGUGAAUUGUAUAACAAUAAAAUUAAGUAAGU ....((((((((((.(((((((((((.((((((..((((..(((......)))..((....))...))))..)))))).))).))........................((((((.........((((((((..(....).))))))))......(((((((((((((((((((.(((((((((.....))).)))))).))))))...))))))).))))))..((.(..(((((..((((((....))))))....)))))..))).........)))))).)))))))))))).))))......(((((((......(((((....((((((((...........((((((.((((........((((...(((((((.((((..(((........)))..((..((((....)))).(((((((....((((((((......)))))))))))))))..)).)))).)))))))))))......))))))))).).....(((..((((.((((((......((..(((((..(((........))).(((((.((((.(((...(.(((((((((....)))))))))....)...)))....)))).)))))...)))))..))..(....))))))).))))))))))))))).((((((((............)))))))).....(((((((((((.((((....)))).)))))))..)))).)))))((((.....)))).......))))))) (-216.94) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 994 140 91 231 182 NC_002950:PG1442|5end_hypothetical_protein_1528030..1528260_NEGATIVE 696 139 91 231 183 NC_002950:PG0831|5end_hypothetical_protein_887403..887633_POSITIVE 438 150 101 108 61 NC_002950:PG1554|5end_hypothetical_protein_1635264..1635494_POSITIVE 409 637 597 100 58 NC_002950:PG2075|5end_hypothetical_protein_2176466..2176696_NEGATIVE 409 637 597 100 58 NC_002950:PG1266|5end_hypothetical_protein_1346987..1347217_NEGATIVE 384 668 621 102 43 NC_002950:PG1801|5end_v-type_ATPase,_subunit_E,_putative_1894946..1895176_POSITIVE 377 698 653 215 162 NC_002950:PG1083|5end_hypothetical_protein_1151834..1152064_NEGATIVE 367 140 94 212 153 NC_002950:PG1163|5end_cobyrinic_acid_a,c-diamide_synthase_1243381..1243611_NEGATIVE 366 309 272 47 9 NC_002950:PG1602|5end_hypothetical_protein_1682144..1682374_NEGATIVE 365 645 609 126 92 NC_002950:PG2087|5end_hypothetical_protein_2186990..2187220_POSITIVE 363 648 602 197 143 NC_002950:PG0746|5end_sensor_histidine_kinase_793903..794133_NEGATIVE 358 140 93 93 33 NC_002950:PG1483|5end_conjugative_transposon_protein_TraE_1559085..1559315_NEGATIVE 353 280 232 201 147 NC_002950:PG1928|5end_50S_ribosomal_protein_L14_2019945..2020175_NEGATIVE 352 411 371 97 53 NC_002950:PG1810|5end_2-oxoglutarate_oxidoreductase,_beta_subunit_1906757..1906987_NEGATIVE 352 430 382 59 1 NC_002950:PG0141|5end_spoOJ_protein_164747..164977_NEGATIVE 351 637 597 100 58 NC_002950:PG1671|5end_hypothetical_protein_1757658..1757888_NEGATIVE 351 637 597 100 58 NC_002950:PG0101|5end_hypothetical_protein_119198..119428_POSITIVE 347 637 597 65 20 NC_002950:PG2106|5end_hypothetical_protein_2214694..2214924_POSITIVE 347 280 236 188 142 NC_002950:PG1659|5end_hypothetical_protein_1743747..1743977_NEGATIVE 347 439 392 165 106 NC_002950:PG0946|5end_ABC_transporter,_ATP-binding_protein_1007396..1007626_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 438 150 101 111 64 NC_002950:PG1553|3end_CobN/magnesium_chelatase_family_protein_1635347..1635497_POSITIVE 372 424 381 81 30 NC_002950:PG1085|3end_hypothetical_protein_1153531..1153681_POSITIVE 365 645 609 126 92 NC_002950:PG2086|3end_hypothetical_protein_2187070..2187220_POSITIVE 363 635 594 85 46 NC_002950:PG1874|3end_hypothetical_protein_1969253..1969403_NEGATIVE 352 129 88 139 100 NC_002950:PG0926|3end_hypothetical_protein_984407..984557_NEGATIVE 347 160 114 142 84 NC_002950:PG0268|3end_tRNA_(5-methylaminomethyl-2-thiouridylate)-methyltransferase_302063..302213_POSITIVE 343 358 312 65 17 NC_002950:PG1246|3end_alanyl-tRNA_synthetase_1324509..1324659_POSITIVE 342 643 603 99 55 NC_002950:PG1596|3end_isoleucyl-tRNA_synthetase,_putative_1678036..1678186_POSITIVE 342 649 606 86 25 NC_002950:PG0468|3end_mannose-6-phosphate_isomerase,_class_I_510374..510524_NEGATIVE 342 450 401 73 21 NC_002950:PG0142|3end_SpoOJ_regulator_protein_164755..164905_NEGATIVE 340 136 101 61 20 NC_002950:PG1089|3end_DNA-binding_response_regulator_RprY_1155523..1155673_NEGATIVE 340 360 314 145 92 NC_002950:PG0412|3end_DNA_mismatch_repair_protein_MutL_449584..449734_NEGATIVE 337 158 117 122 87 NC_002950:PG1494|3end_hypothetical_protein_1571234..1571384_POSITIVE 332 379 334 124 67 NC_002950:PG2032|3end_primosomal_protein_N'_2130868..2131018_NEGATIVE 330 432 392 146 103 NC_002950:PG0947|3end_hypothetical_protein_1007399..1007549_NEGATIVE 327 136 94 70 13 NC_002950:PG1798|3end_immunoreactive_46_kDa_antigen_PG99_1893028..1893178_NEGATIVE 325 658 615 46 9 NC_002950:PG1667|3end_outer_membrane_efflux_protein_1750992..1751142_POSITIVE 325 635 596 83 38 NC_002950:PG0164|3end_hypothetical_protein_187081..187231_NEGATIVE 324 648 604 46 4 NC_002950:PG1476|3end_conjugative_transposon_protein_TraM_1551858..1552008_NEGATIVE 324 155 112 47 1 NC_002950:PG1256|3end_ribonuclease,_Rne/Rng_family_1332748..1332898_NEGATIVE