Origin IGS: cttgttctgtatctgtagaaaagcatttcctgtgcttgtgacggtacgggagtgctataccccgaccattctcttgcgacaggcctattttctatgtttttg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 caaaaacatagaaaataggcctgtcgcaagagaatggtcggggtatagcactcccgtaccgtcacaagcacaggaaatgcttttctacagatacagaacaag Mask Tandem Repeat Region ================================================ cttgttctgtatctgtagaaaagcatttcctgtgcttgtgacggtacgggagtgctataccccgaccattctcttgcgacaggcctattttctatgtttttg Find is-nt database================================================ Query_seq: PG1396:PG1397|PG1396:PG1397:cell shape-determining protein MreB:bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase:<-<-:1478817..1478918 102 cttgttctgtatctgtagaaaagcatttcctgtgcttgtgacggtacgggagtgctataccccgaccattctcttgcgacaggcctattttctatgtttttg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1396:PG1397|PG1396:PG1397:cell shape-determining protein MreB:bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase:<-<-:1478817..1478918 102 cttgttctgtatctgtagaaaagcatttcctgtgcttgtgacggtacgggagtgctataccccgaccattctcttgcgacaggcctattttctatgtttttg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1396:PG1397|PG1396:PG1397:cell shape-determining protein MreB:bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase:<-<-:1478817..1478918 102 cttgttctgtatctgtagaaaagcatttcctgtgcttgtgacggtacgggagtgctataccccgaccattctcttgcgacaggcctattttctatgtttttg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cttgttctgtatctgtagaaaagcatttcctgtgcttgtgacggtacgggagtgctataccccgaccattctcttgcgacaggcctattttctatgtttttg Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 2 End: 100 . M F C I C R K A F P V L V T V R E C Y T P T I L L R Q A Y F L C F .. Protein_Len: 31 Strand: - Start: 3 End: 95 .. T R Y R Y F L M E Q A Q S P V P T S Y G S W E R A V P R N E M ....... cttgttctgtatctgtagaaaagcatttcctgtgcttgtgacggtacgggagtgctataccccgaccattctcttgcgacaggcctattttctatgtttttg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cttgttctgtatctgtagaaaagcatttcctgtgcttgtgacggtacgggagtgctataccccgaccattctcttgcgacaggcctattttctatgtttttg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG1396:PG1397|PG1396:PG1397:cell shape-determining protein MreB:bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase:<-<-:1478817..1478918 102 cttgttctgtatctgtagaaaagcatttcctgtgcttgtgacggtacgggagtgctataccccgaccattctcttgcgacaggcctattttctatgtttttg Intra-Species Hit: Count: 1 Min: 1 Max: 102 Len: 102 Subject: NC_002950_PG1396_PG1397|cell shape-determining protein MreB:bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase|NEGATIVE:NEGATIVE|[1478817,1478918]|102 HSP 1 e-value: 2.0E-51 bit: 202.0 Len: 102 Query Start:1 Query End:102 Subject Strand: POSITIVE Subject Start: 1 Subject End: 102 cttgttctgtatctgtagaaaagcatttcctgtgcttgtgacggtacgggagtgctataccccgaccattctcttgcgacaggcctattttctatgtttttg cttgttctgtatctgtagaaaagcatttcctgtgcttgtgacggtacgggagtgctataccccgaccattctcttgcgacaggcctattttctatgtttttg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUUGUUCUGUAUCUGUAGAAAAGCAUUUCCUGUGCUUGUGACGGUACGGGAGUGCUAUACCCCGACCAUUCUCUUGCGACAGGCCUAUUUUCUAUGUUUUUG ..............((((((((......(((((((..(.((.(((.((((.(((...))))))))))..)).)..)).)))))....))))))))....... (-28.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAAAAACAUAGAAAAUAGGCCUGUCGCAAGAGAAUGGUCGGGGUAUAGCACUCCCGUACCGUCACAAGCACAGGAAAUGCUUUUCUACAGAUACAGAACAAG ........(((((((((..(((((.((..(.((.((((((((((.....)).)))).)))))).)..)))))))...)).)))))))............... (-27.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1057 80 31 171 122 NC_002950:PG1396|5end_cell_shape-determining_protein_MreB_1478726..1478956_NEGATIVE 430 99 53 64 11 NC_002950:PG0930|5end_hypothetical_protein_987542..987772_NEGATIVE 396 67 24 138 96 NC_002950:PG0858|5end_hypothetical_protein_917540..917770_POSITIVE 394 85 41 108 61 NC_002950:PG0608|5end_hypothetical_protein_662945..663175_POSITIVE 394 80 31 64 16 NC_002950:PG0033|5end_RmuC_domain_protein_41540..41770_NEGATIVE 382 69 23 226 169 NC_002950:PG2027|5end_hypothetical_protein_2125494..2125724_POSITIVE 364 78 32 78 32 NC_002950:PG0123|5end_hypothetical_protein_144987..145217_POSITIVE 356 79 43 175 137 NC_002950:PG0525|5end_CTP_synthetase_568999..569229_POSITIVE 345 79 32 219 159 NC_002950:PG0489|5end_polysaccharide_biosynthesis-related_protein_529247..529477_POSITIVE 343 86 41 156 114 NC_002950:PG1967|5end_TPR_domain_protein_2057168..2057398_POSITIVE 343 84 42 117 74 NC_002950:PG0068|5end_hypothetical_protein_81748..81978_POSITIVE 339 78 33 167 125 NC_002950:PG1827|5end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_1920014..1920244_POSITIVE 338 84 41 87 24 NC_002950:PG2030|5end_hypothetical_protein_2129747..2129977_POSITIVE 338 86 42 143 85 NC_002950:PG0657|5end_Maf-like_protein_707782..708012_NEGATIVE 331 78 31 142 91 NC_002950:PG1721|5end_ribonuclease_R_1810284..1810514_POSITIVE 331 87 42 137 81 NC_002950:PG1029|5end_hypothetical_protein_1091080..1091310_POSITIVE 331 85 42 216 161 NC_002950:PG0703|5end_cobalamin_(5'-phosphate)_synthase,_putative_758144..758374_POSITIVE 328 85 42 59 1 NC_002950:PG0714|5end_copper_homeostasis_protein_CutC_768671..768901_NEGATIVE 326 87 43 63 8 NC_002950:PG0021|5end_TIM-barrel_protein,_putative,_NifR3_family_25123..25353_NEGATIVE 325 65 22 187 142 NC_002950:PG1484|5end_hypothetical_protein_1559979..1560209_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 396 67 24 141 99 NC_002950:PG0857|3end_transcriptional_regulator,_putative_917623..917773_POSITIVE 394 85 41 77 30 NC_002950:PG0607|3end_hypothetical_protein_662994..663144_POSITIVE 367 80 32 112 66 NC_002950:PG0555|3end_DNA-binding_protein,_histone-like_family_611716..611866_NEGATIVE 356 79 43 59 21 NC_002950:PG0524|3end_hypothetical_protein_568963..569113_POSITIVE 354 85 41 75 24 NC_002950:PG1649|3end_hypothetical_protein_1730030..1730180_NEGATIVE 338 86 42 127 69 NC_002950:PG0658|3end_phosphatase,_YrbI_family_707798..707948_NEGATIVE 336 79 34 51 1 NC_002950:PG0022|3end_sulfate_permease_family_protein_25120..25270_NEGATIVE 335 67 29 105 48 NC_002950:PG1975|3end_hemagglutinin_protein_HagC_2064350..2064500_POSITIVE 331 87 42 73 17 NC_002950:PG1028|3end_TPR_domain_protein_1091096..1091246_POSITIVE 322 77 31 78 20 NC_002950:PG2044|3end_hypothetical_protein_2144165..2144315_POSITIVE 320 69 21 150 98 NC_002950:PG2003|3end_deoxyguanosinetriphosphate_triphosphohydrolase_2091648..2091798_NEGATIVE 320 86 42 57 15 NC_002950:PG1417|3end_fumarate_hydratase_class_I,_anaerobic_1503021..1503171_POSITIVE 318 55 15 55 14 NC_002950:PG0211|3end_cobalamin_biosynthesis_protein_CbiG/precorrin-4_C11-methyltransferase_245966..246116_NEGATIVE 315 84 41 49 9 NC_002950:PG1152|3end_hypothetical_protein_1234674..1234824_NEGATIVE 312 64 23 146 99 NC_002950:PG0381|3end_cell_volume_regulation_protein_CvrA_411185..411335_NEGATIVE 311 62 29 38 8 NC_002950:PG1595|3end_ribulose-phosphate_3-epimerase_1673594..1673744_NEGATIVE 310 84 42 145 99 NC_002950:PG1687|3end_HIT_family_protein_1773812..1773962_NEGATIVE 309 65 22 40 3 NC_002950:PG1020|3end_hypothetical_protein_1083458..1083608_POSITIVE 307 68 23 69 3 NC_002950:PG0525|3end_CTP_synthetase_570698..570848_POSITIVE 304 79 39 150 102 NC_002950:PG0683|3end_ABC_transporter,_permease_protein,_putative_737701..737851_POSITIVE