Origin IGS: ttttgacccggaaatgtactatcatcaagcagttaccatagtaattgcatgcagcaaatatgatcgctgaaatcaaatacaaacgaccagtgatattattccttatagctggcagtgtcctacaaaagtgtctaagttcctaaagccaatactcatcgtacgcacatgattcaaatatctgttttcgttactct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agagtaacgaaaacagatatttgaatcatgtgcgtacgatgagtattggctttaggaacttagacacttttgtaggacactgccagctataaggaataatatcactggtcgtttgtatttgatttcagcgatcatatttgctgcatgcaattactatggtaactgcttgatgatagtacatttccgggtcaaaa Mask Tandem Repeat Region ================================================ ttttgacccggaaatgtactatcatcaagcagttaccatagtaattgcatgcagcaaatatgatcgctgaaatcaaatacaaacgaccagtgatattattccttatagctggcagtgtcctacaaaagtgtctaagttcctaaagccaatactcatcgtacgcacatgattcaaatatctgttttcgttactct Find is-nt database================================================ Query_seq: PG1357:PG1358|PG1357:PG1358:hypothetical protein:acetyltransferase, GNAT family:<-<-:1436549..1436742 194 ttttgacccggaaatgtactatcatcaagcagttaccatagtaattgcatgcagcaaatatgatcgctgaaatcaaatacaaacgaccagtgatattattccttatagctggcagtgtcctacaaaagtgtctaagttcctaaagccaatactcatcgtacgcacatgattcaaatatctgttttcgttactct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1357:PG1358|PG1357:PG1358:hypothetical protein:acetyltransferase, GNAT family:<-<-:1436549..1436742 194 ttttgacccggaaatgtactatcatcaagcagttaccatagtaattgcatgcagcaaatatgatcgctgaaatcaaatacaaacgaccagtgatattattccttatagctggcagtgtcctacaaaagtgtctaagttcctaaagccaatactcatcgtacgcacatgattcaaatatctgttttcgttactct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1357:PG1358|PG1357:PG1358:hypothetical protein:acetyltransferase, GNAT family:<-<-:1436549..1436742 194 ttttgacccggaaatgtactatcatcaagcagttaccatagtaattgcatgcagcaaatatgatcgctgaaatcaaatacaaacgaccagtgatattattccttatagctggcagtgtcctacaaaagtgtctaagttcctaaagccaatactcatcgtacgcacatgattcaaatatctgttttcgttactct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttttgacccggaaatgtactatcatcaagcagttaccatagtaattgcatgcagcaaatatgatcgctgaaatcaaatacaaacgaccagtgatattattccttatagctggcagtgtcctacaaaagtgtctaagttcctaaagccaatactcatcgtacgcacatgattcaaatatctgttttcgttactct Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 45 End: 140 ............................................ M H A A N M I A E I K Y K R P V I L F L I A G S V L Q K C L S S ...................................................... Protein_Len: 30 Strand: + Start: 77 End: 166 ............................................................................ M Q T T S D I I P Y S W Q C P T K V S K F L K P I L I V R T ............................ Protein_Len: 38 Strand: - Start: 2 End: 115 . K V R F H V I M L C N G Y Y N C A A F I I A S I L Y L R G T I N N R I A P M ............................................................................... ttttgacccggaaatgtactatcatcaagcagttaccatagtaattgcatgcagcaaatatgatcgctgaaatcaaatacaaacgaccagtgatattattccttatagctggcagtgtcctacaaaagtgtctaagttcctaaagccaatactcatcgtacgcacatgattcaaatatctgttttcgttactct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttttgacccggaaatgtactatcatcaagcagttaccatagtaattgcatgcagcaaatatgatcgctgaaatcaaatacaaacgaccagtgatattattccttatagctggcagtgtcctacaaaagtgtctaagttcctaaagccaatactcatcgtacgcacatgattcaaatatctgttttcgttactct Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG1357:PG1358|PG1357:PG1358:hypothetical protein:acetyltransferase, GNAT family:<-<-:1436549..1436742 194 ttttgacccggaaatgtactatcatcaagcagttaccatagtaattgcatgcagcaaatatgatcgctgaaatcaaatacaaacgaccagtgatattattccttatagctggcagtgtcctacaaaagtgtctaagttcctaaagccaatactcatcgtacgcacatgattcaaatatctgttttcgttactct Intra-Species Hit: Count: 1 Min: 1 Max: 194 Len: 194 Subject: NC_002950_PG1357_PG1358|hypothetical protein:acetyltransferase, GNAT family|NEGATIVE:NEGATIVE|[1436549,1436742]|194 HSP 1 e-value: 1.0E-106 bit: 385.0 Len: 194 Query Start:1 Query End:194 Subject Strand: POSITIVE Subject Start: 1 Subject End: 194 ttttgacccggaaatgtactatcatcaagcagttaccatagtaattgcatgcagcaaatatgatcgctgaaatcaaatacaaacgaccagtgatattattccttatagctggcagtgtcctacaaaagtgtctaagttcctaaagccaatactcatcgtacgcacatgattcaaatatctgttttcgttactct ttttgacccggaaatgtactatcatcaagcagttaccatagtaattgcatgcagcaaatatgatcgctgaaatcaaatacaaacgaccagtgatattattccttatagctggcagtgtcctacaaaagtgtctaagttcctaaagccaatactcatcgtacgcacatgattcaaatatctgttttcgttactct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUGACCCGGAAAUGUACUAUCAUCAAGCAGUUACCAUAGUAAUUGCAUGCAGCAAAUAUGAUCGCUGAAAUCAAAUACAAACGACCAGUGAUAUUAUUCCUUAUAGCUGGCAGUGUCCUACAAAAGUGUCUAAGUUCCUAAAGCCAAUACUCAUCGUACGCACAUGAUUCAAAUAUCUGUUUUCGUUACUCU (((((....(((..((((..........((((((((....))))))))...((((..........))))........)))).((..(((((.(((........))).)))))..)).)))..))))).......(((..(.(((((..(((...(((((......))))).....)))..))))).)..))).. (-32.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAGUAACGAAAACAGAUAUUUGAAUCAUGUGCGUACGAUGAGUAUUGGCUUUAGGAACUUAGACACUUUUGUAGGACACUGCCAGCUAUAAGGAAUAAUAUCACUGGUCGUUUGUAUUUGAUUUCAGCGAUCAUAUUUGCUGCAUGCAAUUACUAUGGUAACUGCUUGAUGAUAGUACAUUUCCGGGUCAAAA .((.(...((((...(((..((((((((.(((((.(((((.(((...((..(((....((((....((...((((....)))).))...))))...)))..))))).))))).))))).))).)))))..))).....(((((((((((.((((....)))).)))...))).)))))..))))..).)).... (-39.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 879 130 81 221 172 NC_002950:PG1357|5end_hypothetical_protein_1436458..1436688_NEGATIVE 319 167 127 181 134 NC_002950:PG1599|5end_hypothetical_protein_1679087..1679317_POSITIVE 310 120 74 82 23 NC_002950:PG1819|5end_hypothetical_protein_1914322..1914552_NEGATIVE 288 158 111 197 148 NC_002950:PG0791|5end_adenylate_kinase_843177..843407_NEGATIVE 288 50 7 72 16 NC_002950:PG0034|5end_thioredoxin_42549..42779_NEGATIVE 285 152 111 190 146 NC_002950:PG0308|5end_electron_transport_complex,_RnfABCDGE_type,_A_subunit_343175..343405_POSITIVE 270 118 72 103 39 NC_002950:PG2037|5end_hypothetical_protein_2137812..2138042_NEGATIVE 267 160 111 95 41 NC_002950:PG2186|5end_transcriptional_regulator,_putative_2293619..2293849_NEGATIVE 263 149 105 55 6 NC_002950:PG0708|5end_peptidyl-prolyl_cis-trans_isomerase,_FKBP-type_764628..764858_NEGATIVE 263 168 121 227 175 NC_002950:PG0362|5end_hypothetical_protein_395301..395531_POSITIVE 259 158 115 210 151 NC_002950:PG1395|5end_cell_shape-determining_protein_MreC,_putative_1477669..1477899_NEGATIVE 259 70 22 124 58 NC_002950:PG0425|5end_esterase,_putative_463989..464219_NEGATIVE 258 68 28 53 8 NC_002950:PG1170|5end_SerB_family_protein_1249163..1249393_POSITIVE 256 37 1 67 31 NC_002950:PG1807|5end_v-type_ATPase,_subunit_K_1902069..1902299_POSITIVE 255 69 22 69 12 NC_002950:PG0236|5end_hypothetical_protein_273224..273454_POSITIVE 254 48 8 193 154 NC_002950:PG1853|5end_DNA_polymerase_III,_beta_subunit_1953119..1953349_NEGATIVE 253 160 111 188 139 NC_002950:PG1983|5end_CRISPR-associated_TM1791_family_protein_2073442..2073672_NEGATIVE 253 70 28 54 6 NC_002950:PG1125|5end_hypothetical_protein_1201727..1201957_NEGATIVE 253 89 45 63 30 NC_002950:PG0717|5end_lipoprotein,_putative_771094..771324_NEGATIVE 252 57 19 48 19 NC_002950:PG2220|5end_hypothetical_protein_2334935..2335165_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 270 118 72 106 42 NC_002950:PG2038|3end_N-acetylmuramoyl-L-alanine_amidase,_putative_2137809..2137959_NEGATIVE 252 146 101 68 23 NC_002950:PG1256|3end_ribonuclease,_Rne/Rng_family_1332748..1332898_NEGATIVE 249 126 81 61 12 NC_002950:PG1162|3end_ATP:cob(I)alamin_adenosyltransferase,_putative_1241473..1241623_NEGATIVE 248 148 101 58 21 NC_002950:PG1217|3end_hypothetical_protein_1295987..1296137_POSITIVE 246 120 83 123 71 NC_002950:PG1942|3end_30S_ribosomal_protein_S12_2027946..2028096_NEGATIVE 245 132 108 132 102 NC_002950:PG1067|3end_hypothetical_protein_1134688..1134838_POSITIVE 245 129 84 74 14 NC_002950:PG0338|3end_hypothetical_protein_375137..375287_POSITIVE 244 159 111 91 35 NC_002950:PG1968|3end_hypothetical_protein_2058460..2058610_NEGATIVE 240 127 82 151 103 NC_002950:PG0384|3end_MutS2_family_protein_418697..418847_POSITIVE 237 55 19 90 57 NC_002950:PG1761|3end_acetyltransferase,_GNAT_family_1847739..1847889_POSITIVE 234 146 101 143 105 NC_002950:PG1996|3end_deoxyribose-phosphate_aldolase_2086870..2087020_POSITIVE 234 69 26 139 95 NC_002950:PG1232|3end_glutamate_dehydrogenase_1308274..1308424_NEGATIVE 234 70 23 58 1 NC_002950:PG0702|3end_nicotinate-nucleotide--dimethylbenzimidazole_phosphoribosyltransferase,_putative_758217..758367_POSITIVE 234 146 101 75 20 NC_002950:PG0134|3end_magnesium_transporter_155783..155933_NEGATIVE 230 130 85 124 76 NC_002950:PG0494|3end_hypothetical_protein_535094..535244_NEGATIVE 229 86 50 62 25 NC_002950:PG1143|3end_sugar_dehydrogenase,_UDP-glucose/GDP-mannose_dehydrogenase_family_1225630..1225780_NEGATIVE 227 66 26 50 12 NC_002950:PG1246|3end_alanyl-tRNA_synthetase_1324509..1324659_POSITIVE 227 130 84 109 66 NC_002950:PG0204|3end_hypothetical_protein_240262..240412_NEGATIVE 226 70 26 104 56 NC_002950:PG1229|3end_hypothetical_protein_1305559..1305709_NEGATIVE 225 129 90 49 4 NC_002950:PG1429|3end_hypothetical_protein_1515003..1515153_NEGATIVE