Origin IGS: cttttctcttcacactgcaatttgacacatagaccgaaaaacaagggagagtaccccgattatccaaatcatattcggtattcttttcatcatcgttccttacattttatcgttatacattcactcctgcctttgagggagcaggtagtcattcaaatcctcgtggtcggtatcagagaggaacggtcaatgaccttttctccgaaatatgccctcagcctttccaatgttcttcgtcccgcatcatcattgtcgagatagcattctatcctgcgataaacgcccaacactatcttgcgttgtgggtcgggactatgaagagactttccgatagagactgaatacatgactaacggttcatacaacgactataaaatatattgaacgctgttttgaatgtgtaaatgcctcaaacctgatccgacagaaatgtcgttcgacaaatgaattacctttgttgggtagttataagagaaaaaggca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgcctttttctcttataactacccaacaaaggtaattcatttgtcgaacgacatttctgtcggatcaggtttgaggcatttacacattcaaaacagcgttcaatatattttatagtcgttgtatgaaccgttagtcatgtattcagtctctatcggaaagtctcttcatagtcccgacccacaacgcaagatagtgttgggcgtttatcgcaggatagaatgctatctcgacaatgatgatgcgggacgaagaacattggaaaggctgagggcatatttcggagaaaaggtcattgaccgttcctctctgataccgaccacgaggatttgaatgactacctgctccctcaaaggcaggagtgaatgtataacgataaaatgtaaggaacgatgatgaaaagaataccgaatatgatttggataatcggggtactctcccttgtttttcggtctatgtgtcaaattgcagtgtgaagagaaaag Mask Tandem Repeat Region ================================================ cttttctcttcacactgcaatttgacacatagaccgaaaaacaagggagagtaccccgattatccaaatcatattcggtattcttttcatcatcgttccttacattttatcgttatacattcactcctgcctttgagggagcaggtagtcattcaaatcctcgtggtcggtatcagagaggaacggtcaatgaccttttctccgaaatatgccctcagcctttccaatgttcttcgtcccgcatcatcattgtcgagatagcattctatcctgcgataaacgcccaacactatcttgcgttgtgggtcgggactatgaagagactttccgatagagactgaatacatgactaacggttcatacaacgactataaaatatattgaacgctgttttgaatgtgtaaatgcctcaaacctgatccgacagaaatgtcgttcgacaaatgaattacctttgttgggtagttataagagaaaaaggca Find is-nt database================================================ Query_seq: PG1343:PG1345|PG1343:PG1345:lipoate-protein ligase B:glycosyl transferase, group 1 family protein:->->:1425247..1425729 483 cttttctcttcacactgcaatttgacacatagaccgaaaaacaagggagagtaccccgattatccaaatcatattcggtattcttttcatcatcgttccttacattttatcgttatacattcactcctgcctttgagggagcaggtagtcattcaaatcctcgtggtcggtatcagagaggaacggtcaatgaccttttctccgaaatatgccctcagcctttccaatgttcttcgtcccgcatcatcattgtcgagatagcattctatcctgcgataaacgcccaacactatcttgcgttgtgggtcgggactatgaagagactttccgatagagactgaatacatgactaacggttcatacaacgactataaaatatattgaacgctgttttgaatgtgtaaatgcctcaaacctgatccgacagaaatgtcgttcgacaaatgaattacctttgttgggtagttataagagaaaaaggca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1343:PG1345|PG1343:PG1345:lipoate-protein ligase B:glycosyl transferase, group 1 family protein:->->:1425247..1425729 483 cttttctcttcacactgcaatttgacacatagaccgaaaaacaagggagagtaccccgattatccaaatcatattcggtattcttttcatcatcgttccttacattttatcgttatacattcactcctgcctttgagggagcaggtagtcattcaaatcctcgtggtcggtatcagagaggaacggtcaatgaccttttctccgaaatatgccctcagcctttccaatgttcttcgtcccgcatcatcattgtcgagatagcattctatcctgcgataaacgcccaacactatcttgcgttgtgggtcgggactatgaagagactttccgatagagactgaatacatgactaacggttcatacaacgactataaaatatattgaacgctgttttgaatgtgtaaatgcctcaaacctgatccgacagaaatgtcgttcgacaaatgaattacctttgttgggtagttataagagaaaaaggca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1343:PG1345|PG1343:PG1345:lipoate-protein ligase B:glycosyl transferase, group 1 family protein:->->:1425247..1425729 483 cttttctcttcacactgcaatttgacacatagaccgaaaaacaagggagagtaccccgattatccaaatcatattcggtattcttttcatcatcgttccttacattttatcgttatacattcactcctgcctttgagggagcaggtagtcattcaaatcctcgtggtcggtatcagagaggaacggtcaatgaccttttctccgaaatatgccctcagcctttccaatgttcttcgtcccgcatcatcattgtcgagatagcattctatcctgcgataaacgcccaacactatcttgcgttgtgggtcgggactatgaagagactttccgatagagactgaatacatgactaacggttcatacaacgactataaaatatattgaacgctgttttgaatgtgtaaatgcctcaaacctgatccgacagaaatgtcgttcgacaaatgaattacctttgttgggtagttataagagaaaaaggca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 174 Max: 278 Len: 105 Subject: UniRef90_Q56VG9 Cluster: TraP; n=1; Bacteroides fragilis|Rep: TraP - Bacteroides fragilis HSP 1 e-value: 9.0E-6 bit: 51.6 Len: 105 Query Start:174 Query End:278 Subject Strand: null Subject Start: 239 Subject End: 273 ............................................................................................................................................................................. Y R R I E C Y L D N D D A G R R T L E R L R A Y F G E K V I D R S S L ............................................................................................................................................................................................................. ............................................................................................................................................................................. Y G R I D C Y L D R D E A G R R T L E M L K G H Y E E R V C D R S A L ............................................................................................................................................................................................................. cttttctcttcacactgcaatttgacacatagaccgaaaaacaagggagagtaccccgattatccaaatcatattcggtattcttttcatcatcgttccttacattttatcgttatacattcactcctgcctttgagggagcaggtagtcattcaaatcctcgtggtcggtatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaacgcccaacactatcttgcgttgtgggtcgggactatgaagagactttccgatagagactgaatacatgactaacggttcatacaacgactataaaatatattgaacgctgttttgaatgtgtaaatgcctcaaacctgatccgacagaaatgtcgttcgacaaatgaattacctttgttgggtagttataagagaaaaaggca Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 29 End: 133 ............................ M D R K T R E S T P I I Q I I F G I L F I I V P Y I L S L Y I H S C L .............................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 22 End: 258 ..................... M T H R P K N K G E Y P D Y P N H I R Y S F H H R S L H F I V I H S L L P L R E Q V V I Q I L V V G I R E E R S M T F S ................................................................................................................................................................................................................................. Protein_Len: 41 Strand: + Start: 209 End: 331 ................................................................................................................................................................................................................ M P S A F P M F F V P H H H C R D S I L S C D K R P T L S C V V G R D Y E E T F R ........................................................................................................................................................ Protein_Len: 44 Strand: + Start: 207 End: 338 .............................................................................................................................................................................................................. M C P Q P F Q C S S S R I I I V E I A F Y P A I N A Q H Y L A L W V G T M K R L S D R D ................................................................................................................................................. Protein_Len: 37 Strand: + Start: 371 End: 481 .................................................................................................................................................................................................................................................................................................................................................................................. M K Y I E R C F E C V N A S N L I R Q K C R S T N E L P L L G S Y K R K R .. Protein_Len: 60 Strand: - Start: 156 End: 365 ........................................................................................................................................................... D I V K E G F Y A R L R E L T R R G A D D N D L Y C E I R R Y V G L V I K R Q P D P S H L S K G I S V S Y M V L P E Y M ...................................................................................................................... cttttctcttcacactgcaatttgacacatagaccgaaaaacaagggagagtaccccgattatccaaatcatattcggtattcttttcatcatcgttccttacattttatcgttatacattcactcctgcctttgagggagcaggtagtcattcaaatcctcgtggtcggtatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaacgcccaacactatcttgcgttgtgggtcgggactatgaagagactttccgatagagactgaatacatgactaacggttcatacaacgactataaaatatattgaacgctgttttgaatgtgtaaatgcctcaaacctgatccgacagaaatgtcgttcgacaaatgaattacctttgttgggtagttataagagaaaaaggca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cttttctcttcacactgcaatttgacacatagaccgaaaaacaagggagagtaccccgattatccaaatcatattcggtattcttttcatcatcgttccttacattttatcgttatacattcactcctgcctttgagggagcaggtagtcattcaaatcctcgtggtcggtatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaacgcccaacactatcttgcgttgtgggtcgggactatgaagagactttccgatagagactgaatacatgactaacggttcatacaacgactataaaatatattgaacgctgttttgaatgtgtaaatgcctcaaacctgatccgacagaaatgtcgttcgacaaatgaattacctttgttgggtagttataagagaaaaaggca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 74 HP_score: -6.8 Tail_Score: -4.38154 Start: 185 End: 195 Full_Region: GTATCAGAGAGGAAC GGTC AAT GACC TTTTCTCCGAAATAT ........................................................................................................................................................................................GGTCAATGACC................................................................................................................................................................................................................................................................................................ Find igs database================================================ Query_seq: PG1343:PG1345|PG1343:PG1345:lipoate-protein ligase B:glycosyl transferase, group 1 family protein:->->:1425247..1425729 483 cttttctcttcacactgcaatttgacacatagaccgaaaaacaagggagagtaccccgattatccaaatcatattcggtattcttttcatcatcgttccttacattttatcgttatacattcactcctgcctttgagggagcaggtagtcattcaaatcctcgtggtcggtatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaacgcccaacactatcttgcgttgtgggtcgggactatgaagagactttccgatagagactgaatacatgactaacggttcatacaacgactataaaatatattgaacgctgttttgaatgtgtaaatgcctcaaacctgatccgacagaaatgtcgttcgacaaatgaattacctttgttgggtagttataagagaaaaaggca Intra-Species Hit: Count: 1 Min: 1 Max: 483 Len: 483 Subject: NC_002950_PG1343_PG1345|lipoate-protein ligase B:glycosyl transferase, group 1 family protein|POSITIVE:POSITIVE|[1425247,1425729]|483 HSP 1 e-value: 1.0E-112 bit: 406.0 Len: 205 Query Start:279 Query End:483 Subject Strand: POSITIVE Subject Start: 279 Subject End: 483 ......................................................................................................................................................................................................................................................................................aacgcccaacactatcttgcgttgtgggtcgggactatgaagagactttccgatagagactgaatacatgactaacggttcatacaacgactataaaatatattgaacgctgttttgaatgtgtaaatgcctcaaacctgatccgacagaaatgtcgttcgacaaatgaattacctttgttgggtagttataagagaaaaaggca ......................................................................................................................................................................................................................................................................................aacgcccaacactatcttgcgttgtgggtcgggactatgaagagactttccgatagagactgaatacatgactaacggttcatacaacgactataaaatatattgaacgctgttttgaatgtgtaaatgcctcaaacctgatccgacagaaatgtcgttcgacaaatgaattacctttgttgggtagttataagagaaaaaggca HSP 2 e-value: 4.0E-93 bit: 343.0 Len: 173 Query Start:1 Query End:173 Subject Strand: POSITIVE Subject Start: 1 Subject End: 173 cttttctcttcacactgcaatttgacacatagaccgaaaaacaagggagagtaccccgattatccaaatcatattcggtattcttttcatcatcgttccttacattttatcgttatacattcactcctgcctttgagggagcaggtagtcattcaaatcctcgtggtcggtat...................................................................................................................................................................................................................................................................................................................... cttttctcttcacactgcaatttgacacatagaccgaaaaacaagggagagtaccccgattatccaaatcatattcggtattcttttcatcatcgttccttacattttatcgttatacattcactcctgcctttgagggagcaggtagtcattcaaatcctcgtggtcggtat...................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUUUUCUCUUCACACUGCAAUUUGACACAUAGACCGAAAAACAAGGGAGAGUACCCCGAUUAUCCAAAUCAUAUUCGGUAUUCUUUUCAUCAUCGUUCCUUACAUUUUAUCGUUAUACAUUCACUCCUGCCUUUGAGGGAGCAGGUAGUCAUUCAAAUCCUCGUGGUCGGUAUCAGAGAGGAACGGUCAAUGACCUUUUCUCCGAAAUAUGCCCUCAGCCUUUCCAAUGUUCUUCGUCCCGCAUCAUCAUUGUCGAGAUAGCAUUCUAUCCUGCGAUAAACGCCCAACACUAUCUUGCGUUGUGGGUCGGGACUAUGAAGAGACUUUCCGAUAGAGACUGAAUACAUGACUAACGGUUCAUACAACGACUAUAAAAUAUAUUGAACGCUGUUUUGAAUGUGUAAAUGCCUCAAACCUGAUCCGACAGAAAUGUCGUUCGACAAAUGAAUUACCUUUGUUGGGUAGUUAUAAGAGAAAAAGGCA .(((((((((...(((((......((((((..((((((......(((......))).((((.....))))...)))))).......(((.((.(((((..((.(((((((((((........((((((((((.....)).))))).)))...........((((((((((((((((.((((((.((((...)))).))))))...........(((.............((((((((((((........((((((.(((((....)))))...))))))...((((((.(......).)))).))..))))))...))))))...........))).))).)))).))))).)))).......)))))...)))))).))..))))).))...)))))))))...................(((((....)))))..(((((((.(......)))))))).)))))...)))))))))..... (-124.75) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGCCUUUUUCUCUUAUAACUACCCAACAAAGGUAAUUCAUUUGUCGAACGACAUUUCUGUCGGAUCAGGUUUGAGGCAUUUACACAUUCAAAACAGCGUUCAAUAUAUUUUAUAGUCGUUGUAUGAACCGUUAGUCAUGUAUUCAGUCUCUAUCGGAAAGUCUCUUCAUAGUCCCGACCCACAACGCAAGAUAGUGUUGGGCGUUUAUCGCAGGAUAGAAUGCUAUCUCGACAAUGAUGAUGCGGGACGAAGAACAUUGGAAAGGCUGAGGGCAUAUUUCGGAGAAAAGGUCAUUGACCGUUCCUCUCUGAUACCGACCACGAGGAUUUGAAUGACUACCUGCUCCCUCAAAGGCAGGAGUGAAUGUAUAACGAUAAAAUGUAAGGAACGAUGAUGAAAAGAAUACCGAAUAUGAUUUGGAUAAUCGGGGUACUCUCCCUUGUUUUUCGGUCUAUGUGUCAAAUUGCAGUGUGAAGAGAAAAG .....(((((((((...(((.....(((((((....)).))))).((.(((((....)))))..)).(((((((.((((..((..........((.(((((..((((((((...((((((((((...((....(((.(((.((((((((...((.((..((.(((((...((((((..((.((((((......))))))))((((..(((..((((((....)))))))))....))))...))))))))))))).)).)).)))))))).)))....(((((((...((((...)))).....)))))))....))).....)).......(.(((.((((((........))))))))).)..))))))))))))))))))..))))).))..((((((....((((((...))))))......((((......))))..)))))).))..)))).)))))))..)))...))))))))). (-138.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 454 350 301 94 43 NC_002950:PG2176|5end_ISPg2,_transposase_2284345..2284575_NEGATIVE 454 350 301 94 43 NC_002950:PG1746|5end_ISPg2,_transposase_1832230..1832460_NEGATIVE 454 350 301 94 43 NC_002950:PG1350|5end_ISPg2,_transposase_1430866..1431096_POSITIVE 454 350 301 94 43 NC_002950:PG0865|5end_ISPg2,_transposase_928896..929126_NEGATIVE 454 350 301 94 43 NC_002950:PG0277|5end_ISPg2,_transposase_308856..309086_POSITIVE 388 280 235 166 126 NC_002950:PG0791|5end_adenylate_kinase_843177..843407_NEGATIVE 367 311 271 186 146 NC_002950:PG1769|5end_hypothetical_protein_1856607..1856837_POSITIVE 363 167 126 71 22 NC_002950:PG0265|5end_hypothetical_protein_298551..298781_NEGATIVE 350 155 118 56 11 NC_002950:PG0493|5end_hypothetical_protein_534900..535130_NEGATIVE 348 228 181 187 146 NC_002950:PG2075|5end_hypothetical_protein_2176466..2176696_NEGATIVE 345 199 157 41 3 NC_002950:PG1246|5end_alanyl-tRNA_synthetase_1321799..1322029_POSITIVE 343 139 91 131 80 NC_002950:PG1200|5end_hypothetical_protein_1277992..1278222_NEGATIVE 338 254 211 70 18 NC_002950:PG0736|5end_ribonuclease_HII_785608..785838_POSITIVE 337 330 281 73 26 NC_002950:PG2126|5end_conserved_hypothetical_protein_TIGR00044_2233112..2233342_NEGATIVE 336 189 141 136 73 NC_002950:PG1640|5end_DNA-damage-inducible_protein_F_1721245..1721475_NEGATIVE 335 220 180 57 15 NC_002950:PG0771|5end_hypothetical_protein_824524..824754_POSITIVE 333 267 221 48 1 NC_002950:PG0976|5end_phosphoribosylaminoimidazole-succinocarboxamide_synthase_1038326..1038556_POSITIVE 332 273 232 68 19 NC_002950:PG0021|5end_TIM-barrel_protein,_putative,_NifR3_family_25123..25353_NEGATIVE 329 168 121 96 35 NC_002950:PG1783|5end_glycosyl_transferase,_group_2_family_protein_1871868..1872098_POSITIVE 329 260 211 197 148 NC_002950:PG0150|5end_conserved_hypothetical_protein_TIGR01125_173544..173774_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 352 207 161 143 78 NC_002950:PG0431|3end_hypothetical_protein_469174..469324_POSITIVE 350 309 266 151 101 NC_002950:PG0555|3end_DNA-binding_protein,_histone-like_family_611716..611866_NEGATIVE 348 196 158 56 2 NC_002950:PG0621|3end_hypothetical_protein_676020..676170_POSITIVE 338 258 216 149 108 NC_002950:PG0800|3end_hypothetical_protein_854719..854869_NEGATIVE 338 254 211 59 7 NC_002950:PG0735|3end_aminotransferase,_class_V_785677..785827_POSITIVE 337 330 281 76 29 NC_002950:PG2127|3end_hypothetical_protein_2233109..2233259_NEGATIVE 336 189 141 121 58 NC_002950:PG1641|3end_phosphotyrosine_protein_phosphatase_1721260..1721410_NEGATIVE 332 273 232 71 22 NC_002950:PG0022|3end_sulfate_permease_family_protein_25120..25270_NEGATIVE 331 220 174 126 70 NC_002950:PG2040|3end_DNA-binding_protein,_histone-like_family_2138398..2138548_NEGATIVE 329 168 121 109 48 NC_002950:PG1782|3end_hypothetical_protein_1871961..1872111_POSITIVE 328 423 384 151 114 NC_002950:PG1134|3end_thioredoxin_reductase_1215371..1215521_POSITIVE 327 201 161 74 38 NC_002950:PG1211|3end_hexapeptide_transferase_family_protein_1291459..1291609_POSITIVE 324 198 153 39 1 NC_002950:PG2073|3end_hypothetical_protein_2174219..2174369_NEGATIVE 324 198 153 39 1 NC_002950:PG1669|3end_hypothetical_protein_1755411..1755561_NEGATIVE 324 198 153 39 1 NC_002950:PG1264|3end_hypothetical_protein_1344740..1344890_NEGATIVE 324 198 153 39 1 NC_002950:PG0103|3end_hypothetical_protein_121525..121675_POSITIVE 323 181 141 73 30 NC_002950:PG1684|3end_hypothetical_protein_1771483..1771633_POSITIVE 319 170 122 132 78 NC_002950:PG1484|3end_hypothetical_protein_1559275..1559425_NEGATIVE 318 217 172 54 6 NC_002950:PG2004|3end_hypothetical_protein_2093135..2093285_NEGATIVE 318 252 211 130 100 NC_002950:PG0330|3end_DNA-binding_protein,_histone-like_family_364478..364628_NEGATIVE