Origin IGS: agttctttgaaacgtttcttagaagtatctgatttcaaaagccggtcccaaattcgaaaccatgctctacaaagataaagaaaaggcgatgtgtactgaaaaatgacatcatcaccatcttcacgtgtcttctttctattataagaaatgatgcttcaaatacgacaaggggagtcgaggtcgtcgatctgctattgagtctgaacttcccaaacaaggggggaaacgaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgtttttcaatctctgcacgggctgtcatcacagccgacatggttcggagttcatggccttcgataaaaatcatatcgatattcaccggtaaatggaaaacatgaaatatgacaggcaacgtgaagtggactactccatcataacactgaaaaacggaaatacgataacagttcgtcgtaagcctgtaattggtctggggacattccacaaacacagtgcggtatcatcttggaaaatcagcatattgtttatgtacaaggattccacttcgtgagatgaaaaagggtatgggagataaaaagttgcagtccatgaaactatagagggcaatgccgacttattcggagcaaaatagggacgcagatcccatgcaatgacgagccatgctgtgctcaatgaaggcggatcgtaagaaaacgattttgtaacttccaatagtaagtgcaacactttctacagaaaaattcccgacgaggctagcctcgtcggggaataaaatcaatcgtttgatttcgtgttgcatttatcatattaagggtttggacaaagaaagagaagagacattctgcgctatctttgcttgcag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctgcaagcaaagatagcgcagaatgtctcttctctttctttgtccaaacccttaatatgataaatgcaacacgaaatcaaacgattgattttattccccgacgaggctagcctcgtcgggaatttttctgtagaaagtgttgcacttactattggaagttacaaaatcgttttcttacgatccgccttcattgagcacagcatggctcgtcattgcatgggatctgcgtccctattttgctccgaataagtcggcattgccctctatagtttcatggactgcaactttttatctcccataccctttttcatctcacgaagtggaatccttgtacataaacaatatgctgattttccaagatgataccgcactgtgtttgtggaatgtccccagaccaattacaggcttacgacgaactgttatcgtatttccgtttttcagtgttatgatggagtagtccacttcacgttgcctgtcatatttcatgttttccatttaccggtgaatatcgatatgatttttatcgaaggccatgaactccgaaccatgtcggctgtgatgacagcccgtgcagagattgaaaaacgatcgtaggcgtacgacgccttcctgcacggtgcaggagaccagcgtaggcctacggtgctcattacaacatgctaattatcagtgcattatggccaattaaagtaaaacgagaacgaccttagaaattttccctgttttgcgtcttcaaaattcgtttccccccttgtttgggaagttcagactcaatagcagatcgacgacctcgactccccttgtcgtatttgaagcatcatttcttataatagaaagaagacacgtgaagatggtgatgatgtcatttttcagtacacatcgccttttctttatctttgtagagcatggtttcgaatttgggaccggcttttgaaatcagatacttctaagaaacgtttcaaagaact Mask Tandem Repeat Region ================================================ agttctttgaaacgtttcttagaagtatctgatttcaaaagccggtcccaaattcgaaaccatgctctacaaagataaagaaaaggcgatgtgtactgaaaaatgacatcatcaccatcttcacgtgtcttctttctattataagaaatgatgcttcaaatacgacaaggggagtcgaggtcgtcgatctgctattgagtctgaacttcccaaacaaggggggaaacgaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgtttttcaatctctgcacgggctgtcatcacagccgacatggttcggagttcatggccttcgataaaaatcatatcgatattcaccggtaaatggaaaacatgaaatatgacaggcaacgtgaagtggactactccatcataacactgaaaaacggaaatacgataacagttcgtcgtaagcctgtaattggtctggggacattccacaaacacagtgcggtatcatcttggaaaatcagcatattgtttatgtacaaggattccacttcgtgagatgaaaaagggtatgggagataaaaagttgcagtccatgaaactatagagggcaatgccgacttattcggagcaaaatagggacgcagatcccatgcaatgacgagccatgctgtgctcaatgaaggcggatcgtaagaaaacgattttgtaacttccaatagtaagtgcaacactttctacagaaaaattcccgacgaggctagcctcgtcggggaataaaatcaatcgtttgatttcgtgttgcatttatcatattaagggtttggacaaagaaagagaagagacattctgcgctatctttgcttgcag Find is-nt database================================================ Query_seq: PG1335:PG1337|PG1335:PG1337:hypothetical protein:umuD protein:<-->:1416985..1417952 968 agttctttgaaacgtttcttagaagtatctgatttcaaaagccggtcccaaattcgaaaccatgctctacaaagataaagaaaaggcgatgtgtactgaaaaatgacatcatcaccatcttcacgtgtcttctttctattataagaaatgatgcttcaaatacgacaaggggagtcgaggtcgtcgatctgctattgagtctgaacttcccaaacaaggggggaaacgaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgtttttcaatctctgcacgggctgtcatcacagccgacatggttcggagttcatggccttcgataaaaatcatatcgatattcaccggtaaatggaaaacatgaaatatgacaggcaacgtgaagtggactactccatcataacactgaaaaacggaaatacgataacagttcgtcgtaagcctgtaattggtctggggacattccacaaacacagtgcggtatcatcttggaaaatcagcatattgtttatgtacaaggattccacttcgtgagatgaaaaagggtatgggagataaaaagttgcagtccatgaaactatagagggcaatgccgacttattcggagcaaaatagggacgcagatcccatgcaatgacgagccatgctgtgctcaatgaaggcggatcgtaagaaaacgattttgtaacttccaatagtaagtgcaacactttctacagaaaaattcccgacgaggctagcctcgtcggggaataaaatcaatcgtttgatttcgtgttgcatttatcatattaagggtttggacaaagaaagagaagagacattctgcgctatctttgcttgcag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1335:PG1337|PG1335:PG1337:hypothetical protein:umuD protein:<-->:1416985..1417952 968 agttctttgaaacgtttcttagaagtatctgatttcaaaagccggtcccaaattcgaaaccatgctctacaaagataaagaaaaggcgatgtgtactgaaaaatgacatcatcaccatcttcacgtgtcttctttctattataagaaatgatgcttcaaatacgacaaggggagtcgaggtcgtcgatctgctattgagtctgaacttcccaaacaaggggggaaacgaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgtttttcaatctctgcacgggctgtcatcacagccgacatggttcggagttcatggccttcgataaaaatcatatcgatattcaccggtaaatggaaaacatgaaatatgacaggcaacgtgaagtggactactccatcataacactgaaaaacggaaatacgataacagttcgtcgtaagcctgtaattggtctggggacattccacaaacacagtgcggtatcatcttggaaaatcagcatattgtttatgtacaaggattccacttcgtgagatgaaaaagggtatgggagataaaaagttgcagtccatgaaactatagagggcaatgccgacttattcggagcaaaatagggacgcagatcccatgcaatgacgagccatgctgtgctcaatgaaggcggatcgtaagaaaacgattttgtaacttccaatagtaagtgcaacactttctacagaaaaattcccgacgaggctagcctcgtcggggaataaaatcaatcgtttgatttcgtgttgcatttatcatattaagggtttggacaaagaaagagaagagacattctgcgctatctttgcttgcag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1335:PG1337|PG1335:PG1337:hypothetical protein:umuD protein:<-->:1416985..1417952 968 agttctttgaaacgtttcttagaagtatctgatttcaaaagccggtcccaaattcgaaaccatgctctacaaagataaagaaaaggcgatgtgtactgaaaaatgacatcatcaccatcttcacgtgtcttctttctattataagaaatgatgcttcaaatacgacaaggggagtcgaggtcgtcgatctgctattgagtctgaacttcccaaacaaggggggaaacgaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgtttttcaatctctgcacgggctgtcatcacagccgacatggttcggagttcatggccttcgataaaaatcatatcgatattcaccggtaaatggaaaacatgaaatatgacaggcaacgtgaagtggactactccatcataacactgaaaaacggaaatacgataacagttcgtcgtaagcctgtaattggtctggggacattccacaaacacagtgcggtatcatcttggaaaatcagcatattgtttatgtacaaggattccacttcgtgagatgaaaaagggtatgggagataaaaagttgcagtccatgaaactatagagggcaatgccgacttattcggagcaaaatagggacgcagatcccatgcaatgacgagccatgctgtgctcaatgaaggcggatcgtaagaaaacgattttgtaacttccaatagtaagtgcaacactttctacagaaaaattcccgacgaggctagcctcgtcggggaataaaatcaatcgtttgatttcgtgttgcatttatcatattaagggtttggacaaagaaagagaagagacattctgcgctatctttgcttgcag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agttctttgaaacgtttcttagaagtatctgatttcaaaagccggtcccaaattcgaaaccatgctctacaaagataaagaaaaggcgatgtgtactgaaaaatgacatcatcaccatcttcacgtgtcttctttctattataagaaatgatgcttcaaatacgacaaggggagtcgaggtcgtcgatctgctattgagtctgaacttcccaaacaaggggggaaacgaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgtttttcaatctctgcacgggctgtcatcacagccgacatggttcggagttcatggccttcgataaaaatcatatcgatattcaccggtaaatggaaaacatgaaatatgacaggcaacgtgaagtggactactccatcataacactgaaaaacggaaatacgataacagttcgtcgtaagcctgtaattggtctggggacattccacaaacacagtgcggtatcatcttggaaaatcagcatattgtttatgtacaaggattccacttcgtgagatgaaaaagggtatgggagataaaaagttgcagtccatgaaactatagagggcaatgccgacttattcggagcaaaatagggacgcagatcccatgcaatgacgagccatgctgtgctcaatgaaggcggatcgtaagaaaacgattttgtaacttccaatagtaagtgcaacactttctacagaaaaattcccgacgaggctagcctcgtcggggaataaaatcaatcgtttgatttcgtgttgcatttatcatattaagggtttggacaaagaaagagaagagacattctgcgctatctttgcttgcag Predict ORF larger than 30AA ================================================ Protein_Len: 53 Strand: + Start: 75 End: 233 .......................................................................... M K K R R C V L K N D I I T I F T C L L S I I R N D A S N T T R G V E V V D L L L S L N F P N K G G N E F ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 33 Strand: + Start: 347 End: 445 .......................................................................................................................................................................................................................................................................................................................................................... M H R A G R R R T P T I V F Q S L H G L S S Q P T W F G V H G L R ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 44 Strand: + Start: 339 End: 470 .................................................................................................................................................................................................................................................................................................................................................. M V S C T V Q E G V V R L R S F F N L C T G C H H S R H G S E F M A F D K N H I D I H R .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 55 Strand: + Start: 319 End: 483 .............................................................................................................................................................................................................................................................................................................................. M S T V G L R W S P A P C R K A S Y A Y D R F S I S A R A V I T A D M V R S S W P S I K I I S I F T G K W K T ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 474 End: 653 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M E N M K Y D R Q R E V D Y S I I T L K N G N T I T V R R K P V I G L G T F H K H S A V S S W K I S I L F M Y K D S T S ........................................................................................................................................................................................................................................................................................................................... Protein_Len: 44 Strand: + Start: 571 End: 702 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M V W G H S T N T V R Y H L G K S A Y C L C T R I P L R E M K K G M G D K K L Q S M K L .......................................................................................................................................................................................................................................................................... Protein_Len: 50 Strand: + Start: 817 End: 966 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M V S A T L S T E K F P T R L A S S G N K I N R L I S C C I Y H I K G L D K E R E E T F C A I F A C .. Protein_Len: 60 Strand: - Start: 723 End: 905 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. S C F L S A S G M C H R A M S H E I F A S R L F V I K Y S G I T L A V S E V S F N G V L S A E D P F L I L R K I E H Q M ............................................................... Protein_Len: 35 Strand: - Start: 609 End: 713 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ R P F D A Y Q K H V L I G S R S I F F P I P S L F N C D M F S Y L A M ............................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 515 End: 832 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. H S S F P Y P L Y F T A T W S V I S P L A S K N P A F Y P R L D W A I V L W A T S L S P P D Y S F S K T V E L L L H L M ........................................................................................................................................ Protein_Len: 60 Strand: - Start: 378 End: 596 ......................................................................................................................................................................................................................................................................................................................................................................................... S M T R L E H G E I F I M D I N V P L H F V H F I V P L T F H V V G D Y C Q F V S I R Y C N T T L R Y N T Q P C E V F M .................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 39 Strand: - Start: 284 End: 400 ........................................................................................................................................................................................................................................................................................... N A M I C Q Y N A H Q L S C R L G V S T E Q V T C S P T T R R R D N K L R Q M ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 36 Strand: - Start: 142 End: 249 ............................................................................................................................................. L F H H K L Y S L P L R P R R D A I S D S S G L C P P F R I K F V C F M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 38 End: 256 ..................................... F F L R H T S F F S M M V M K V H R R E I I L F S A E F V V L P T S T T S R S N L R F K G F L P P F S N Q L R L V P F M ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 50 Strand: - Start: 3 End: 152 .. E K F R K K S T D S K L L R D W I R F W A R C L Y L F P S T Y Q F I V D D G D E R T K K R N Y S I M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ agttctttgaaacgtttcttagaagtatctgatttcaaaagccggtcccaaattcgaaaccatgctctacaaagataaagaaaaggcgatgtgtactgaaaaatgacatcatcaccatcttcacgtgtcttctttctattataagaaatgatgcttcaaatacgacaaggggagtcgaggtcgtcgatctgctattgagtctgaacttcccaaacaaggggggaaacgaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgtttttcaatctctgcacgggctgtcatcacagccgacatggttcggagttcatggccttcgataaaaatcatatcgatattcaccggtaaatggaaaacatgaaatatgacaggcaacgtgaagtggactactccatcataacactgaaaaacggaaatacgataacagttcgtcgtaagcctgtaattggtctggggacattccacaaacacagtgcggtatcatcttggaaaatcagcatattgtttatgtacaaggattccacttcgtgagatgaaaaagggtatgggagataaaaagttgcagtccatgaaactatagagggcaatgccgacttattcggagcaaaatagggacgcagatcccatgcaatgacgagccatgctgtgctcaatgaaggcggatcgtaagaaaacgattttgtaacttccaatagtaagtgcaacactttctacagaaaaattcccgacgaggctagcctcgtcggggaataaaatcaatcgtttgatttcgtgttgcatttatcatattaagggtttggacaaagaaagagaagagacattctgcgctatctttgcttgcag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agttctttgaaacgtttcttagaagtatctgatttcaaaagccggtcccaaattcgaaaccatgctctacaaagataaagaaaaggcgatgtgtactgaaaaatgacatcatcaccatcttcacgtgtcttctttctattataagaaatgatgcttcaaatacgacaaggggagtcgaggtcgtcgatctgctattgagtctgaacttcccaaacaaggggggaaacgaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgtttttcaatctctgcacgggctgtcatcacagccgacatggttcggagttcatggccttcgataaaaatcatatcgatattcaccggtaaatggaaaacatgaaatatgacaggcaacgtgaagtggactactccatcataacactgaaaaacggaaatacgataacagttcgtcgtaagcctgtaattggtctggggacattccacaaacacagtgcggtatcatcttggaaaatcagcatattgtttatgtacaaggattccacttcgtgagatgaaaaagggtatgggagataaaaagttgcagtccatgaaactatagagggcaatgccgacttattcggagcaaaatagggacgcagatcccatgcaatgacgagccatgctgtgctcaatgaaggcggatcgtaagaaaacgattttgtaacttccaatagtaagtgcaacactttctacagaaaaattcccgacgaggctagcctcgtcggggaataaaatcaatcgtttgatttcgtgttgcatttatcatattaagggtttggacaaagaaagagaagagacattctgcgctatctttgcttgcag Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 80 HP_score: -10.2 Tail_Score: -3.42985 Start: 324 End: 382 Full_Region: GCATGTTGTAATGAG CACCGTAGGCCTACGCTGGTCTCCTGCA CCG TGCAGGAAGGCGTCGTACGCCTACGATC GTTTTTCAATCTCTG ...................................................................................................................................................................................................................................................................................................................................CACCGTAGGCCTACGCTGGTCTCCTGCACCGTGCAGGAAGGCGTCGTACGCCTACGATC.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -12.8 Tail_Score: -3.27476 Start: 326 End: 380 Full_Region: ATGTTGTAATGAGCA CCGTAGGCCTACGCTGGTCTCCTGCA CCG TGCAGGAAGGCGTCGTACGCCTACGA TCGTTTTTCAATCTC .....................................................................................................................................................................................................................................................................................................................................CCGTAGGCCTACGCTGGTCTCCTGCACCGTGCAGGAAGGCGTCGTACGCCTACGA............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 100 HP_score: -16.3 Tail_Score: -2.55763 Start: 327 End: 379 Full_Region: TGTTGTAATGAGCAC CGTAGGCCTACGCTGGTCTCCTGCA CCG TGCAGGAAGGCGTCGTACGCCTACG ATCGTTTTTCAATCT ......................................................................................................................................................................................................................................................................................................................................CGTAGGCCTACGCTGGTCTCCTGCACCGTGCAGGAAGGCGTCGTACGCCTACG............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 44 HP_score: -3.2 Tail_Score: -3.03243 Start: 442 End: 460 Full_Region: CGGAGTTCATGGCCT TCGATA AAAATCA TATCGA TATTCACCGGTAAAT .........................................................................................................................................................................................................................................................................................................................................................................................................................................................TCGATAAAAATCATATCGA............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 100 HP_score: -15.6 Tail_Score: -4.96848 Start: 847 End: 874 Full_Region: aacgattgattttat tccccgacgagg ctag cctcgtcgggaa tttttctgtagaaag ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tccccgacgaggctagcctcgtcgggaa.............................................................................................. TransTerm Strand: - Conf: 100 HP_score: -18.2 Tail_Score: -2.74094 Start: 849 End: 872 Full_Region: cgattgattttattc cccgacgagg ctag cctcgtcggg aatttttctgtagaa ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cccgacgaggctagcctcgtcggg................................................................................................ Find igs database================================================ Query_seq: PG1335:PG1337|PG1335:PG1337:hypothetical protein:umuD protein:<-->:1416985..1417952 968 agttctttgaaacgtttcttagaagtatctgatttcaaaagccggtcccaaattcgaaaccatgctctacaaagataaagaaaaggcgatgtgtactgaaaaatgacatcatcaccatcttcacgtgtcttctttctattataagaaatgatgcttcaaatacgacaaggggagtcgaggtcgtcgatctgctattgagtctgaacttcccaaacaaggggggaaacgaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgtttttcaatctctgcacgggctgtcatcacagccgacatggttcggagttcatggccttcgataaaaatcatatcgatattcaccggtaaatggaaaacatgaaatatgacaggcaacgtgaagtggactactccatcataacactgaaaaacggaaatacgataacagttcgtcgtaagcctgtaattggtctggggacattccacaaacacagtgcggtatcatcttggaaaatcagcatattgtttatgtacaaggattccacttcgtgagatgaaaaagggtatgggagataaaaagttgcagtccatgaaactatagagggcaatgccgacttattcggagcaaaatagggacgcagatcccatgcaatgacgagccatgctgtgctcaatgaaggcggatcgtaagaaaacgattttgtaacttccaatagtaagtgcaacactttctacagaaaaattcccgacgaggctagcctcgtcggggaataaaatcaatcgtttgatttcgtgttgcatttatcatattaagggtttggacaaagaaagagaagagacattctgcgctatctttgcttgcag Intra-Species Hit: Count: 5 Min: 1 Max: 968 Len: 968 Subject: NC_002950_PG1335_PG1337|hypothetical protein:umuD protein|NEGATIVE:POSITIVE|[1416985,1417952]|968 HSP 1 e-value: 0.0 bit: 1919.0 Len: 968 Query Start:1 Query End:968 Subject Strand: POSITIVE Subject Start: 1 Subject End: 968 agttctttgaaacgtttcttagaagtatctgatttcaaaagccggtcccaaattcgaaaccatgctctacaaagataaagaaaaggcgatgtgtactgaaaaatgacatcatcaccatcttcacgtgtcttctttctattataagaaatgatgcttcaaatacgacaaggggagtcgaggtcgtcgatctgctattgagtctgaacttcccaaacaaggggggaaacgaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgtttttcaatctctgcacgggctgtcatcacagccgacatggttcggagttcatggccttcgataaaaatcatatcgatattcaccggtaaatggaaaacatgaaatatgacaggcaacgtgaagtggactactccatcataacactgaaaaacggaaatacgataacagttcgtcgtaagcctgtaattggtctggggacattccacaaacacagtgcggtatcatcttggaaaatcagcatattgtttatgtacaaggattccacttcgtgagatgaaaaagggtatgggagataaaaagttgcagtccatgaaactatagagggcaatgccgacttattcggagcaaaatagggacgcagatcccatgcaatgacgagccatgctgtgctcaatgaaggcggatcgtaagaaaacgattttgtaacttccaatagtaagtgcaacactttctacagaaaaattcccgacgaggctagcctcgtcggggaataaaatcaatcgtttgatttcgtgttgcatttatcatattaagggtttggacaaagaaagagaagagacattctgcgctatctttgcttgcag agttctttgaaacgtttcttagaagtatctgatttcaaaagccggtcccaaattcgaaaccatgctctacaaagataaagaaaaggcgatgtgtactgaaaaatgacatcatcaccatcttcacgtgtcttctttctattataagaaatgatgcttcaaatacgacaaggggagtcgaggtcgtcgatctgctattgagtctgaacttcccaaacaaggggggaaacgaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgtttttcaatctctgcacgggctgtcatcacagccgacatggttcggagttcatggccttcgataaaaatcatatcgatattcaccggtaaatggaaaacatgaaatatgacaggcaacgtgaagtggactactccatcataacactgaaaaacggaaatacgataacagttcgtcgtaagcctgtaattggtctggggacattccacaaacacagtgcggtatcatcttggaaaatcagcatattgtttatgtacaaggattccacttcgtgagatgaaaaagggtatgggagataaaaagttgcagtccatgaaactatagagggcaatgccgacttattcggagcaaaatagggacgcagatcccatgcaatgacgagccatgctgtgctcaatgaaggcggatcgtaagaaaacgattttgtaacttccaatagtaagtgcaacactttctacagaaaaattcccgacgaggctagcctcgtcggggaataaaatcaatcgtttgatttcgtgttgcatttatcatattaagggtttggacaaagaaagagaagagacattctgcgctatctttgcttgcag Subject: NC_002950_PG0013_PG0016|hypothetical protein:sigma-54 dependent DNA-binding response regulator|POSITIVE:POSITIVE|[16502,17086]|585 HSP 1 e-value: 2.0E-80 bit: 301.0 Len: 230 Query Start:155 Query End:384 Subject Strand: NEGATIVE Subject Start: 154 Subject End: 385 ..........................................................................................................................................................ttcaaatacgacaaggggagtcgaggtcgtcgatctgctattgagtc-tgaacttcccaaacaaggggggaaac-gaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgt........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ ..........................................................................................................................................................ttcaaatacgacaaggagagtcgaggtcatcgatctgctattgagtcctgatcttcccaaacaaggggggaaaatgaattttgaagacgcgaaattgggaaaatttccaaggtcgttctcgttttactttaattgtccataatgtactggtaattagcatgttgtaatgagcaccgtacgcctacgctggtctcctgcatcgtgcaggagtgcatcgtacgcctacgatcgt........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Subject: NC_002950_PG0759_PG0762|TPR domain protein:trigger factor, putative|NEGATIVE:POSITIVE|[810876,812898]|2023 HSP 1 e-value: 1.0E-69 bit: 266.0 Len: 249 Query Start:131 Query End:379 Subject Strand: POSITIVE Subject Start: 1431 Subject End: 1679 ..................................................................................................................................tctttctattataagaaatgatgcttcaaatacgacaaggggagtcgaggtcgtcgatctgctattgagtctgaacttcccaaacaaggggggaaac-gaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacg............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. ..................................................................................................................................tctttctattataagaaatgatacttcgaatacgacaaggg-agtcgaggtcgtcgatctgctattgagtcaaagcttcccaaacaaggggaaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggctataatgtactggtaattagcatgttgtaattagtactgtaggcctacgctggtctcctgcaccgtgcaggaaggtttcgtacgcctacg............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 2.0E-37 bit: 159.0 Len: 123 Query Start:327 Query End:449 Subject Strand: POSITIVE Subject Start: 1668 Subject End: 1791 ......................................................................................................................................................................................................................................................................................................................................cgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgtttttcaatctctgcacgggctgtcatcacagccgacatgg-ttcggagttcatggccttcgataaa....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ......................................................................................................................................................................................................................................................................................................................................cgtacgcctacgctggtctcctgcatcgtgcaggagagtatcgtacgtctacgatcgtttttcaatctctgcacgggctgtcatcacaaccgacatggtttcggagttcatggctttcgataaa....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_002950_PG1186_PG1189|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[1269242,1270131]|890 HSP 1 e-value: 1.0E-54 bit: 216.0 Len: 184 Query Start:201 Query End:384 Subject Strand: POSITIVE Subject Start: 284 Subject End: 468 ........................................................................................................................................................................................................ctgaacttcccaaacaaggggggaaac-gaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgt........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ ........................................................................................................................................................................................................ctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgt........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Subject: NC_002950_PG1566_PG1570|glutamyl-tRNA synthetase:rhodanese-like domain protein|NEGATIVE:NEGATIVE|[1647492,1648671]|1180 HSP 1 e-value: 2.0E-47 bit: 192.0 Len: 184 Query Start:201 Query End:384 Subject Strand: NEGATIVE Subject Start: 533 Subject End: 717 ........................................................................................................................................................................................................ctgaacttcccaaacaaggggggaaac-gaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgt........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ ........................................................................................................................................................................................................ctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgtaacgagcaccgtacgcctacgccggtctcctgcaccgtgcaggagagcatcgtacgcctacgatcgt........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUUCUUUGAAACGUUUCUUAGAAGUAUCUGAUUUCAAAAGCCGGUCCCAAAUUCGAAACCAUGCUCUACAAAGAUAAAGAAAAGGCGAUGUGUACUGAAAAAUGACAUCAUCACCAUCUUCACGUGUCUUCUUUCUAUUAUAAGAAAUGAUGCUUCAAAUACGACAAGGGGAGUCGAGGUCGUCGAUCUGCUAUUGAGUCUGAACUUCCCAAACAAGGGGGGAAACGAAUUUUGAAGACGCAAAACAGGGAAAAUUUCUAAGGUCGUUCUCGUUUUACUUUAAUUGGCCAUAAUGCACUGAUAAUUAGCAUGUUGUAAUGAGCACCGUAGGCCUACGCUGGUCUCCUGCACCGUGCAGGAAGGCGUCGUACGCCUACGAUCGUUUUUCAAUCUCUGCACGGGCUGUCAUCACAGCCGACAUGGUUCGGAGUUCAUGGCCUUCGAUAAAAAUCAUAUCGAUAUUCACCGGUAAAUGGAAAACAUGAAAUAUGACAGGCAACGUGAAGUGGACUACUCCAUCAUAACACUGAAAAACGGAAAUACGAUAACAGUUCGUCGUAAGCCUGUAAUUGGUCUGGGGACAUUCCACAAACACAGUGCGGUAUCAUCUUGGAAAAUCAGCAUAUUGUUUAUGUACAAGGAUUCCACUUCGUGAGAUGAAAAAGGGUAUGGGAGAUAAAAAGUUGCAGUCCAUGAAACUAUAGAGGGCAAUGCCGACUUAUUCGGAGCAAAAUAGGGACGCAGAUCCCAUGCAAUGACGAGCCAUGCUGUGCUCAAUGAAGGCGGAUCGUAAGAAAACGAUUUUGUAACUUCCAAUAGUAAGUGCAACACUUUCUACAGAAAAAUUCCCGACGAGGCUAGCCUCGUCGGGGAAUAAAAUCAAUCGUUUGAUUUCGUGUUGCAUUUAUCAUAUUAAGGGUUUGGACAAAGAAAGAGAAGAGACAUUCUGCGCUAUCUUUGCUUGCAG ...((((..(((((((((((.((((((((..(((((...(((.(((..(......)..)))..))).....((((((..(((..((.(((((((.(........)))).)))).))...)))...)))))).............)))))))))))))...........(((((((..(((.(.(((((.....)))))))))..))))))).........))))))))..)))..)))).((((..((((((...(((((...(((....(((((((.(((..(((.(((...((((...........)))).......((((((((((((((.((((...(((((((((.....))))).))))..)))).))))))).((((((((((.......(((((.(((((((.....((((......))))..((((((....((((((.......))))))......(((.....)))....))))))..))))))).)..))))..(((((....)))))........)))))))))).............(((((((((..((((((...(((..((....))..))).....)))).))....(((((((((((((((.(((((.....))))).....))))....))).))))))))......((((((((.........((((.((((......((....)).((....((((.....)))).)).......)))))))).)))))))).)))))))))......))))))).....))).)))...))))))))))......(((((..(((((((((((((((((..............((((((((((((....))))))))))))...(((((((....))))))).)))))))))))))..))))..)))))..)))..))))).((((......))))......)))))).)))).. (-290.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUGCAAGCAAAGAUAGCGCAGAAUGUCUCUUCUCUUUCUUUGUCCAAACCCUUAAUAUGAUAAAUGCAACACGAAAUCAAACGAUUGAUUUUAUUCCCCGACGAGGCUAGCCUCGUCGGGAAUUUUUCUGUAGAAAGUGUUGCACUUACUAUUGGAAGUUACAAAAUCGUUUUCUUACGAUCCGCCUUCAUUGAGCACAGCAUGGCUCGUCAUUGCAUGGGAUCUGCGUCCCUAUUUUGCUCCGAAUAAGUCGGCAUUGCCCUCUAUAGUUUCAUGGACUGCAACUUUUUAUCUCCCAUACCCUUUUUCAUCUCACGAAGUGGAAUCCUUGUACAUAAACAAUAUGCUGAUUUUCCAAGAUGAUACCGCACUGUGUUUGUGGAAUGUCCCCAGACCAAUUACAGGCUUACGACGAACUGUUAUCGUAUUUCCGUUUUUCAGUGUUAUGAUGGAGUAGUCCACUUCACGUUGCCUGUCAUAUUUCAUGUUUUCCAUUUACCGGUGAAUAUCGAUAUGAUUUUUAUCGAAGGCCAUGAACUCCGAACCAUGUCGGCUGUGAUGACAGCCCGUGCAGAGAUUGAAAAACGAUCGUAGGCGUACGACGCCUUCCUGCACGGUGCAGGAGACCAGCGUAGGCCUACGGUGCUCAUUACAACAUGCUAAUUAUCAGUGCAUUAUGGCCAAUUAAAGUAAAACGAGAACGACCUUAGAAAUUUUCCCUGUUUUGCGUCUUCAAAAUUCGUUUCCCCCCUUGUUUGGGAAGUUCAGACUCAAUAGCAGAUCGACGACCUCGACUCCCCUUGUCGUAUUUGAAGCAUCAUUUCUUAUAAUAGAAAGAAGACACGUGAAGAUGGUGAUGAUGUCAUUUUUCAGUACACAUCGCCUUUUCUUUAUCUUUGUAGAGCAUGGUUUCGAAUUUGGGACCGGCUUUUGAAAUCAGAUACUUCUAAGAAACGUUUCAAAGAACU ......(((((((.((.(.(((.....))).).)).))))))).................(((.((((((((((((((((....))))))))....((((((((((....))))))))))................)))))))).)))...(((((((((......(((((((((((((((..(((.....(((((((.((((((((.(((((..((.(((((....))))).........((((.....(((((((.(((.((.((((..((((.(((.(((((....(((.....)))................((((((((....((((......))))..........(((((..(((((((.((.(((((((((.((.....)).))))).....)))))).(((((..(....)..))))).............)))))))..)))))...))))))))..)))))..))).....((((((((.((.......)).)))))).)).))))...)))).)).))).)))....))))......)))).))..))))))).))))))...(((((.....)))))((((((.((((.....(((((((.....))))))).....)))).)))))).))))))).......((((...........))))...)))..)))...))))...)))))))).(((((((.........(((((((((((((.......(((((..(((......))).......(((.....((..((((((.....)))).))..)).)))......)))))....(((((......)))))))))))..(((((((.(((((((.((((........).))))))))))...)))))))....))))))).((((((((((.(((....)))..)))))))))).....))))))).))).))))))...... (-275.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 915 90 41 181 132 NC_002950:PG1335|5end_hypothetical_protein_1416894..1417124_NEGATIVE 558 388 343 211 165 NC_002950:PG1975|5end_hemagglutinin_protein_HagC_2063218..2063448_POSITIVE 459 379 331 174 118 NC_002950:PG1474|5end_conjugative_transposon_protein_TraO_1550816..1551046_NEGATIVE 426 380 333 152 106 NC_002950:PG1977|5end_hypothetical_protein_2066693..2066923_NEGATIVE 423 365 321 55 1 NC_002950:PG0675|5end_indolepyruvate_ferredoxin_oxidoreductase,_alpha_subunit_726436..726666_NEGATIVE 401 750 704 45 1 NC_002950:PG1338|5end_umuC_protein_1418246..1418476_POSITIVE 401 360 311 154 110 NC_002950:PG0522|5end_tRNA_delta(2)-isopentenylpyrophosphate_transferase_566901..567131_NEGATIVE 400 380 332 51 3 NC_002950:PG1869|5end_hypothetical_protein_1965822..1966052_NEGATIVE 394 379 331 50 8 NC_002950:PG1479|5end_conjugative_transposon_protein_TraJ_1555234..1555464_NEGATIVE 393 369 322 189 137 NC_002950:PG2029|5end_hypothetical_protein_2126543..2126773_POSITIVE 393 370 323 78 24 NC_002950:PG1992|5end_glucose-inhibited_division_protein_A_2081410..2081640_POSITIVE 386 448 402 211 168 NC_002950:PG0447|5end_hypothetical_protein_487278..487508_POSITIVE 383 408 363 206 155 NC_002950:PG2173|5end_outer_membrane_lipoprotein_Omp28_2280813..2281043_NEGATIVE 383 380 331 70 11 NC_002950:PG1230|5end_hypothetical_protein_1308068..1308298_NEGATIVE 383 390 341 63 8 NC_002950:PG0429|5end_pyruvate_synthase_466100..466330_POSITIVE 380 440 391 158 99 NC_002950:PG0607|5end_hypothetical_protein_662552..662782_POSITIVE 380 369 326 181 129 NC_002950:PG0541|5end_hypothetical_protein_594757..594987_POSITIVE 379 378 338 146 99 NC_002950:PG2021|5end_hypothetical_protein_2114852..2115082_NEGATIVE 379 440 395 165 110 NC_002950:PG1921|5end_ribosomal_protein_S5_2017021..2017251_NEGATIVE 377 368 322 47 5 NC_002950:PG2188|5end_diaminopimelate_decarboxylase_2295781..2296011_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 944 378 332 135 89 NC_002950:PG1027|3end_hypothetical_protein_1089197..1089347_NEGATIVE 462 858 812 145 100 NC_002950:PG1861|3end_hypothetical_protein_1959887..1960037_NEGATIVE 426 380 333 122 76 NC_002950:PG1978|3end_hypothetical_protein_2066723..2066873_NEGATIVE 389 369 322 148 96 NC_002950:PG0361|3end_hypothetical_protein_395481..395631_POSITIVE 380 375 333 140 75 NC_002950:PG0143|3end_hydrolase,_carbon-nitrogen_family_165747..165897_NEGATIVE 379 378 338 142 95 NC_002950:PG2022|3end_hypothetical_protein_2114856..2115006_NEGATIVE 376 380 331 89 28 NC_002950:PG1620|3end_carboxyl-terminal_protease-related_protein_1699415..1699565_NEGATIVE 376 444 401 119 58 NC_002950:PG0498|3end_S-ribosylhomocysteinase_538527..538677_POSITIVE 372 379 332 106 53 NC_002950:PG1972|3end_hemagglutinin_protein_HagB_2061203..2061353_POSITIVE 371 440 394 144 86 NC_002950:PG1715|3end_hypothetical_protein_1803393..1803543_NEGATIVE 367 377 333 87 48 NC_002950:PG2172|3end_hypothetical_protein_2279191..2279341_NEGATIVE 367 438 395 148 104 NC_002950:PG1922|3end_ribosomal_protein_L18_2017027..2017177_NEGATIVE 367 366 325 59 12 NC_002950:PG0413|3end_hypothetical_protein_451437..451587_NEGATIVE 364 369 323 64 19 NC_002950:PG0752|3end_uracil_phosphoribosyltransferase_798397..798547_POSITIVE 364 444 401 60 7 NC_002950:PG0606|3end_hypothetical_protein_662451..662601_NEGATIVE 364 370 323 119 65 NC_002950:PG0523|3end_inositol-5-monophosphate_dehydrogenase_566898..567048_NEGATIVE 364 380 335 70 29 NC_002950:PG0174|3end_pyridine_nucleotide-disulphide_oxidoreductase_family_protein_194812..194962_NEGATIVE 359 439 391 85 30 NC_002950:PG2102|3end_immunoreactive_61_kDa_antigen_PG91_2211424..2211574_NEGATIVE 359 788 748 41 1 NC_002950:PG0020|3end_transcriptional_regulator,_MarR_family_24166..24316_POSITIVE 357 369 321 128 94 NC_002950:PG1497|3end_DNA-binding_protein,_histone-like_family_1574635..1574785_POSITIVE